Restriction map for
pFN21AA0449
(Circular) (Six-base) MAPSORT of: pFN21AA0449.seq Check: 1999 from: 1 to: 9577
pFN21AA0449 9577 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5648 7387 278
Size: 53 83 186 936 4112 1739 2468
Fragments arranged by size:
4112 2468 1739 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6920 1937
Size: 4983 4594
Acc65I G'GTAC_C
Cuts at: 6898 6898
Size: 9577
AclI AA'CG_TT
Cuts at: 1563 7704 8077 1563
Size: 6141 373 3063
Fragments arranged by size:
6141 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 2864 4259 4711 5109 5277 7640 9024
Size: 1004 228 1395 452 398 168 2363 1384
Cuts at: 9024 9292 1632
Size: 268 1917
Fragments arranged by size:
2363 1917 1395 1384 1004 452 398 268
228 168
AfeI AGC'GCT
Cuts at: 5799 5799
Size: 9577
AflII C'TTAA_G
Cuts at: 820 1017 4208 820
Size: 197 3191 6189
Fragments arranged by size:
6189 3191 197
AflIII A'CryG_T
Cuts at: 1203 8476 9453 1203
Size: 7273 977 1327
Fragments arranged by size:
7273 1327 977
AgeI A'CCGG_T
Cuts at: 8408 8408
Size: 9577
AhdI GACnn_n'nnGTC
Cuts at: 8306 8306
Size: 9577
AleI CACnn'nnGTG
Cuts at: 1166 2204 5012 5060 5702 5860 1166
Size: 1038 2808 48 642 158 4883
Fragments arranged by size:
4883 2808 1038 642 158 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3177 1184
Size: 1993 7584
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4838 4870 4838
Size: 32 9545
AlwNI CAG_nnn'CTG
Cuts at: 2125 2446 2614 3646 3949 4174 4759 4936 5076
Size: 321 168 1032 303 225 585 177 140
Cuts at: 5076 6654 6783 7320 8892 2125
Size: 1578 129 537 1572 2810
Fragments arranged by size:
2810 1578 1572 1032 585 537 321 303
225 177 168 140 129
ApaI G_GGCC'C
Cuts at: 2629 3106 3304 4594 5468 2629
Size: 477 198 1290 874 6738
Fragments arranged by size:
6738 1290 874 477 198
ApaLI G'TGCA_C
Cuts at: 2719 2957 3162 5841 7634 8790 2719
Size: 238 205 2679 1793 1156 3506
Fragments arranged by size:
3506 2679 1793 1156 238 205
ApoI r'AATT_y
Cuts at: 1493 6886 7112 7264 9373 1493
Size: 5393 226 152 2109 1697
Fragments arranged by size:
5393 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8131 160
Size: 872 7099 1606
Fragments arranged by size:
7099 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9577
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2344 2779 3692 3838 3878 4910
Size: 73 164 237 435 913 146 40 1032
Cuts at: 4910 5267 5604 5631 6043 6130 6256 6528 6628
Size: 357 337 27 412 87 126 272 100
Cuts at: 6628 6902 1870
Size: 274 4545
Fragments arranged by size:
4545 1032 913 435 412 357 337 274
272 237 164 146 126 100 87 73
40 27
AvrII C'CTAG_G
Cuts at: 5621 5621
Size: 9577
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2077 2110 2077
Size: 33 9544
BamHI G'GATC_C
Cuts at: 2046 6907 7274 2046
Size: 4861 367 4349
Fragments arranged by size:
4861 4349 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4473 4676 6236 6437 6898
Size: 325 217 627 2686 203 1560 201 461
Cuts at: 6898 8353 618
Size: 1455 1842
Fragments arranged by size:
2686 1842 1560 1455 627 461 325 217
203 201
BanII G_rGCy'C
Cuts at: 1391 2118 2454 2629 2672 3050 3106 3304 3545
Size: 727 336 175 43 378 56 198 241
Cuts at: 3545 3692 4405 4594 4910 5421 5468 6468 6573
Size: 147 713 189 316 511 47 1000 105
Cuts at: 6573 6896 1391
Size: 323 4072
Fragments arranged by size:
4072 1000 727 713 511 378 336 323
316 241 198 189 175 147 105 56
47 43
BbeI G_GCGC'C
Cuts at: 4477 4477
Size: 9577
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2155 2288 4454 5752 928
Size: 971 256 133 2166 1298 4753
Fragments arranged by size:
4753 2166 1298 971 256 133
BbvCI CC'TCA_GC
Cuts at: 3246 4079 4548 4941 6224 3246
Size: 833 469 393 1283 6599
Fragments arranged by size:
6599 1283 833 469 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2200 2234 7768 7802 1903
Size: 34 263 34 5534 34 3678
Fragments arranged by size:
5534 3678 263 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7469 8679 7469
Size: 1210 8367
BclI T'GATC_A
Cuts at: 1548 2922 1548
Size: 1374 8203
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3101 3575 4259
Size: 107 122 71 631 605 1429 474 684
Cuts at: 4259 4283 6234 6324 8188 136
Size: 24 1951 90 1864 1525
Fragments arranged by size:
1951 1864 1525 1429 684 631 605 474
122 107 90 71 24
BglII A'GATC_T
Cuts at: 2190 5331 9572 2190
Size: 3141 4241 2195
Fragments arranged by size:
4241 3141 2195
BlpI GC'TnA_GC
Cuts at: 6284 6647 6994 6284
Size: 363 347 8867
Fragments arranged by size:
8867 363 347
Bme1580I G_kGCm'C
Cuts at: 1790 2300 2398 2629 2723 2961 3106 3166 3304
Size: 510 98 231 94 238 145 60 138
Cuts at: 3304 4594 5468 5845 6239 6440 7638 8794 1790
Size: 1290 874 377 394 201 1198 1156 2573
Fragments arranged by size:
2573 1290 1198 1156 874 510 394 377
238 231 201 145 138 98 94 60
BmgBI CAC'GTC
Cuts at: 1318 6126 1318
Size: 4808 4769
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2434 2763 5356
Size: 416 53 83 399 24 1017 329 2593
Cuts at: 5356 6613 7308 8266 442
Size: 1257 695 958 1753
Fragments arranged by size:
2593 1753 1257 1017 958 695 416 399
329 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3398 1056
Size: 2342 7235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 3631 5062 5350 2902
Size: 729 1431 288 7129
Fragments arranged by size:
7129 1431 729 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2981 3549 3668 3983 4073 4427 4588
Size: 1229 513 568 119 315 90 354 161
Cuts at: 4588 4781 4832 5042 6760 8237 1239
Size: 193 51 210 1718 1477 2579
Fragments arranged by size:
2579 1718 1477 1229 568 513 354 315
210 193 161 119 90 51
Bpu10I CC'TnA_GC
Cuts at: 3246 4079 4548 4941 6192 6224 3246
Size: 833 469 393 1251 32 6599
Fragments arranged by size:
6599 1251 833 469 393 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3977 4349 4730 5102 7702 8567 8865 3977
Size: 372 381 372 2600 865 298 4689
Fragments arranged by size:
4689 2600 865 381 372 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3065 4328 5021 5550 5584 6025 8240
Size: 617 1566 1263 693 529 34 441 2215
Cuts at: 8240 882
Size: 2219
Fragments arranged by size:
2219 2215 1566 1263 693 617 529 441
34
BsaAI yAC'GTr
Cuts at: 493 1204 7368 493
Size: 711 6164 2702
Fragments arranged by size:
6164 2702 711
BsaBI GATnn'nnATC
Cuts at: 3935 9571 3935
Size: 5636 3941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2568 4474 5238 5645
Size: 53 83 186 936 1035 1906 764 407
Cuts at: 5645 7384 7766 8401 275
Size: 1739 382 635 1451
Fragments arranged by size:
1906 1739 1451 1035 936 764 635 407
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8010 8408 8682 8829 9240 8010
Size: 398 274 147 411 8347
Fragments arranged by size:
8347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2960 2990 4838 4868 6005
Size: 30 165 30 1557 30 1848 30 1137
Cuts at: 6005 6035 1178
Size: 30 4720
Fragments arranged by size:
4720 1848 1557 1137 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2342 2477 2612 2984 3476 3758 3782
Size: 1153 3 135 135 372 492 282 24
Cuts at: 3782 3785 3788 3818 3821 3896 4118 4322 4456
Size: 3 3 30 3 75 222 204 134
Cuts at: 4456 4862 4958 5039 5051 5203 5342 5844 1186
Size: 406 96 81 12 152 139 502 4919
Fragments arranged by size:
4919 1153 502 492 406 372 282 222
204 152 139 135 135 134 96 81
75 30 24 12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2055 2128 2724 3007 3363 6418 8780 9276 2055
Size: 73 596 283 356 3055 2362 496 2356
Fragments arranged by size:
3055 2362 2356 596 496 356 283 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 4449 4533 5036 5315 6730 1163
Size: 573 948 1765 84 503 279 1415 4010
Fragments arranged by size:
4010 1765 1415 948 573 503 279 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4063 5677 7027 7788 8816 1540
Size: 275 186 2062 1614 1350 761 1028 2301
Fragments arranged by size:
2301 2062 1614 1350 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2454 2672 2723 2961 3166 4197 4405 5845 6193
Size: 218 51 238 205 1031 208 1440 348
Cuts at: 6193 6573 7638 7723 8794 2454
Size: 380 1065 85 1071 3237
Fragments arranged by size:
3237 1440 1071 1065 1031 380 348 238
218 208 205 85 51
BsmI GAATG_Cn'
Cuts at: 3280 6111 7088 7181 3280
Size: 2831 977 93 5676
Fragments arranged by size:
5676 2831 977 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3740 3780 5636 5774 3740
Size: 40 1856 138 7543
Fragments arranged by size:
7543 1856 138 40
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2300 2398 2454 2629 2672 2723
Size: 399 328 182 98 56 175 43 51
Cuts at: 2723 2961 3050 3106 3166 3304 3545 3692 4197
Size: 238 89 56 60 138 241 147 505
Cuts at: 4197 4405 4594 4910 5421 5468 5845 6193 6239
Size: 208 189 316 511 47 377 348 46
Cuts at: 6239 6440 6468 6573 6896 7638 7723 8794 1391
Size: 201 28 105 323 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 511 505 399 377 348
328 323 316 241 238 208 201 189
182 175 147 138 105 98 89 85
60 56 56 51 47 46 43 28
BspEI T'CCGG_A
Cuts at: 9240 9240
Size: 9577
BspHI T'CATG_A
Cuts at: 3030 3369 7466 9196 3030
Size: 339 4097 1730 3411
Fragments arranged by size:
4097 3411 1730 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2505 3396 4173 6279 6932 844
Size: 1661 891 777 2106 653 3489
Fragments arranged by size:
3489 2106 1661 891 777 653
BsrBI CCG'CTC
Cuts at: 1394 1823 2839 2869 3652 4498 4596 4828 4891
Size: 429 1016 30 783 846 98 232 63
Cuts at: 4891 7464 1394
Size: 2573 3507
Fragments arranged by size:
3507 2573 1016 846 783 429 232 98
63 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 2373 2923 3421 5752 5761 6268 8072
Size: 1183 1125 550 498 2331 9 507 1804
Cuts at: 8072 8246 65
Size: 174 1396
Fragments arranged by size:
2331 1804 1396 1183 1125 550 507 498
174 9
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2828 3404 8221 8408 1700
Size: 110 199 819 576 4817 187 2869
Fragments arranged by size:
4817 2869 819 576 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9577
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9577
BssSI C'ACGA_G
Cuts at: 2534 6374 7637 8649 2534
Size: 3840 1263 1012 3462
Fragments arranged by size:
3840 3462 1263 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4174 4511 794
Size: 3380 337 5860
Fragments arranged by size:
5860 3380 337
BstBI TT'CG_AA
Cuts at: 8381 8381
Size: 9577
BstEII G'GTnAC_C
Cuts at: 1184 6346 1184
Size: 5162 4415
BstXI CCAn_nnnn'nTGG
Cuts at: 2254 3604 5506 5704 6016 2254
Size: 1350 1902 198 312 5815
Fragments arranged by size:
5815 1902 1350 312 198
BstYI r'GATC_y
Cuts at: 1972 2046 2190 2466 2529 4035 4395 5166 5331
Size: 74 144 276 63 1506 360 771 165
Cuts at: 5331 6907 7274 7659 7676 9128 9214 9226 9572
Size: 1576 367 385 17 1452 86 12 346
Cuts at: 9572 1972
Size: 1977
Fragments arranged by size:
1977 1576 1506 1452 771 385 367 360
346 276 165 144 86 74 63 17
12
Bsu36I CC'TnA_GG
Cuts at: 2932 4439 2932
Size: 1507 8070
BtgI C'CryG_G
Cuts at: 513 1065 2004 2247 2292 3603 4086 5061 5871
Size: 552 939 243 45 1311 483 975 810
Cuts at: 5871 513
Size: 4219
Fragments arranged by size:
4219 1311 975 939 810 552 483 243
45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2338 3423 487
Size: 683 56 1112 1085 6641
Fragments arranged by size:
6641 1112 1085 683 56
BtsI GCAGTG_nn'
Cuts at: 2799 3164 3850 4871 5855 5867 7093 7887 7907
Size: 365 686 1021 984 12 1226 794 20
Cuts at: 7907 2799
Size: 4469
Fragments arranged by size:
4469 1226 1021 984 794 686 365 20
12
ClaI AT'CG_AT
Cuts at: 1554 3339 1554
Size: 1785 7792
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9542
DraI TTT'AAA
Cuts at: 6881 6881
Size: 9577
DraIII CAC_nnn'GTG
Cuts at: 2818 2818
Size: 9577
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2027 5756 6124 8584 809
Size: 507 711 3729 368 2460 1802
Fragments arranged by size:
3729 2460 1802 711 507 368
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2011 2037
Size: 54 1425 50 275 18 177 4 26
Cuts at: 2037 2826 3912 4479 4557 5874 6316 6357 6846
Size: 789 1086 567 78 1317 442 41 489
Cuts at: 6846 7024 7913 9542 8
Size: 178 889 1629 43
Fragments arranged by size:
1629 1425 1317 1086 889 789 567 489
442 275 178 177 78 54 50 43
41 26 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 7024 1537
Size: 275 5212 4090
Fragments arranged by size:
5212 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3680 3774 3843 4133 4911 7507 1353
Size: 2327 94 69 290 778 2596 3423
Fragments arranged by size:
3423 2596 2327 778 290 94 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3779 3920 8164 8538 8684 387
Size: 1064 138 2190 141 4244 374 146 1280
Fragments arranged by size:
4244 2190 1280 1064 374 146 141 138
EcoICRI GAG'CTC
Cuts at: 2452 2670 4403 6571 2452
Size: 218 1733 2168 5458
Fragments arranged by size:
5458 2168 1733 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 2636 2864 2981 3549 3668 3983
Size: 393 836 168 228 117 568 119 315
Cuts at: 3983 4073 4259 4427 4588 4711 4781 4832 5042
Size: 90 186 168 161 123 70 51 210
Cuts at: 5042 5109 5277 6760 7640 8237 9024 9292 1239
Size: 67 168 1483 880 597 787 268 1524
Fragments arranged by size:
1524 1483 880 836 787 597 568 393
315 268 228 210 186 168 168 168
161 123 119 117 90 70 67 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 3535 4862 4865 2618
Size: 917 1327 3 7330
Fragments arranged by size:
7330 1327 917 3
EcoO109I rG'GnC_Cy
Cuts at: 2122 2625 2626 3300 3301 3643 4162 4590 5278
Size: 503 1 674 1 342 519 428 688
Cuts at: 5278 5465 6553 2122
Size: 187 1088 5146
Fragments arranged by size:
5146 1088 688 674 519 503 428 342
187 1 1
EcoRI G'AATT_C
Cuts at: 6886 7264 6886
Size: 378 9199
EcoRV GAT'ATC
Cuts at: 5479 5479
Size: 9577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2830 3721 6781 2830
Size: 891 3060 5626
Fragments arranged by size:
5626 3060 891
FseI GG_CCGG'CC
Cuts at: 2013 2013
Size: 9577
FspI TGC'GCA
Cuts at: 2714 6594 8083 2714
Size: 3880 1489 4208
Fragments arranged by size:
4208 3880 1489
FspAI rTGC'GCAy
Cuts at: 2714 6594 2714
Size: 3880 5697
HaeII r_GCGC'y
Cuts at: 1678 2392 2439 2931 3246 4477 5801 6654 8724
Size: 714 47 492 315 1231 1324 853 2070
Cuts at: 8724 1678
Size: 2531
Fragments arranged by size:
2531 2070 1324 1231 853 714 492 315
47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3821 3853 3854 3886 4387
Size: 32 135 32 2249 32 1 32 501
Cuts at: 4387 4419 4568 4600 5564 5596 5970 5977 6002
Size: 32 149 32 964 32 374 7 25
Cuts at: 6002 6009 8218 8250 8292 8324 1373
Size: 7 2209 32 42 32 2626
Fragments arranged by size:
2626 2249 2209 964 501 374 149 135
42 32 32 32 32 32 32 32
32 32 25 7 7 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4807 6921 7167 669
Size: 988 281 2869 2114 246 3079
Fragments arranged by size:
3079 2869 2114 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3476 748
Size: 2728 6849
HpaI GTT'AAC
Cuts at: 7167 7167
Size: 9577
KasI G'GCGC_C
Cuts at: 4473 4473
Size: 9577
KpnI G_GTAC'C
Cuts at: 6902 6902
Size: 9577
MfeI C'AATT_G
Cuts at: 7176 7176
Size: 9577
MluI A'CGCG_T
Cuts at: 9453 9453
Size: 9577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 2544 3751 5157 5806 8691 8875 1305
Size: 996 243 1207 1406 649 2885 184 2007
Fragments arranged by size:
2885 2007 1406 1207 996 649 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4481 6318 6848 10
Size: 54 1425 343 2649 1837 530 2739
Fragments arranged by size:
2739 2649 1837 1425 530 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2654 4002 4784 5012 5060 5702
Size: 648 1038 450 1348 782 228 48 642
Cuts at: 5702 5860 6080 6477 7535 7894 8053 518
Size: 158 220 397 1058 359 159 2042
Fragments arranged by size:
2042 1348 1058 1038 782 648 642 450
397 359 228 220 159 158 48
MspA1I CmG'CkG
Cuts at: 1606 2059 3679 3976 4051 4318 4378 5020 5653
Size: 453 1620 297 75 267 60 642 633
Cuts at: 5653 6993 7670 8818 9063 1606
Size: 1340 677 1148 245 2120
Fragments arranged by size:
2120 1620 1340 1148 677 642 633 453
297 267 245 75 60
NaeI GCC'GGC
Cuts at: 2011 2830 3406 2011
Size: 819 576 8182
Fragments arranged by size:
8182 819 576
NarI GG'CG_CC
Cuts at: 4474 4474
Size: 9577
NcoI C'CATG_G
Cuts at: 513 1065 2004 2292 3603 4086 5871 513
Size: 552 939 288 1311 483 1785 4219
Fragments arranged by size:
4219 1785 1311 939 552 483 288
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9577
NgoMIV G'CCGG_C
Cuts at: 2009 2828 3404 2009
Size: 819 576 8182
Fragments arranged by size:
8182 819 576
NheI G'CTAG_C
Cuts at: 1052 3394 1052
Size: 2342 7235
NotI GC'GGCC_GC
Cuts at: 7024 7024
Size: 9577
NspI r_CATG'y
Cuts at: 1132 1229 2719 6935 8480 1132
Size: 97 1490 4216 1545 2229
Fragments arranged by size:
4216 2229 1545 1490 97
PasI CC'CwG_GG
Cuts at: 1348 3553 1348
Size: 2205 7372
PciI A'CATG_T
Cuts at: 8476 8476
Size: 9577
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2446 4645 1108
Size: 1338 2199 6040
Fragments arranged by size:
6040 2199 1338
PfoI T'CCnGG_A
Cuts at: 2997 3450 5232 6093 2997
Size: 453 1782 861 6481
Fragments arranged by size:
6481 1782 861 453
PmeI GTTT'AAAC
Cuts at: 6881 6881
Size: 9577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4838 4870 7646 7678 9159 9191 4838
Size: 32 2776 32 1481 32 5224
Fragments arranged by size:
5224 2776 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 3643 4162 6553 2122
Size: 1521 519 2391 5146
Fragments arranged by size:
5146 2391 1521 519
PshAI GACnn'nnGTC
Cuts at: 1613 4802 1613
Size: 3189 6388
PsiI TTA'TAA
Cuts at: 7147 7147
Size: 9577
PspOMI G'GGCC_C
Cuts at: 2625 3102 3300 4590 5464 2625
Size: 477 198 1290 874 6738
Fragments arranged by size:
6738 1290 874 477 198
PspXI vC'TCGA_Gb
Cuts at: 2779 2779
Size: 9577
PstI C_TGCA'G
Cuts at: 830 3192 4029 4074 4170 4179 4866 4899 4932
Size: 2362 837 45 96 9 687 33 33
Cuts at: 4932 5277 5862 6038 6929 830
Size: 345 585 176 891 3478
Fragments arranged by size:
3478 2362 891 837 687 585 345 176
96 45 33 33 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9577
PvuII CAG'CTG
Cuts at: 2059 4051 5020 2059
Size: 1992 969 6616
Fragments arranged by size:
6616 1992 969
SacI G_AGCT'C
Cuts at: 2454 2672 4405 6573 2454
Size: 218 1733 2168 5458
Fragments arranged by size:
5458 2168 1733 218
SalI G'TCGA_C
Cuts at: 1936 6919 1936
Size: 4983 4594
SapI GCTCTTCn'nnn_
Cuts at: 3680 3774 3680
Size: 94 9483
SbfI CC_TGCA'GG
Cuts at: 4866 6929 4866
Size: 2063 7514
ScaI AGT'ACT
Cuts at: 6032 7825 9537 6032
Size: 1793 1712 6072
Fragments arranged by size:
6072 1793 1712
SexAI A'CCwGG_T
Cuts at: 2733 2733
Size: 9577
SfcI C'TryA_G
Cuts at: 826 1045 2968 3188 3253 3284 4025 4070 4166
Size: 219 1923 220 65 31 741 45 96
Cuts at: 4166 4175 4247 4862 4895 4928 5273 5858 6034
Size: 9 72 615 33 33 345 585 176
Cuts at: 6034 6925 8060 8741 8932 826
Size: 891 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 891 741 681 615 585
345 220 219 191 176 96 72 65
45 33 33 31 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4283 6324 4283
Size: 2041 7536
SfoI GGC'GCC
Cuts at: 4475 4475
Size: 9577
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9577
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9577
SmaI CCC'GGG
Cuts at: 1872 2109 5606 6630 6904 1872
Size: 237 3497 1024 274 4545
Fragments arranged by size:
4545 3497 1024 274 237
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2779 3992 4208 4364 4745 5117
Size: 197 926 836 1213 216 156 381 372
Cuts at: 5117 5631 7681 8582 8844 820
Size: 514 2050 901 262 1553
Fragments arranged by size:
2050 1553 1213 926 901 836 514 381
372 262 216 197 156
SnaBI TAC'GTA
Cuts at: 493 7368 493
Size: 6875 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9577
SphI G_CATG'C
Cuts at: 1132 6935 1132
Size: 5803 3774
SspI AAT'ATT
Cuts at: 5 52 7501 5
Size: 47 7449 2081
Fragments arranged by size:
7449 2081 47
StuI AGG'CCT
Cuts at: 1517 2144 1517
Size: 627 8950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 2874 2910 3399 3603 4086
Size: 552 939 288 582 36 489 204 483
Cuts at: 4086 4278 5031 5621 5835 5871 6390 7016 513
Size: 192 753 590 214 36 519 626 3074
Fragments arranged by size:
3074 939 753 626 590 582 552 519
489 483 288 214 204 192 36 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 2738 3072 3377 3545 4543 5691
Size: 1255 1086 72 334 305 168 998 1148
Cuts at: 5691 5961 6365 6595 6715 7604 7621 7774 7959
Size: 270 404 230 120 889 17 153 185
Cuts at: 7959 8404 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1148 1086 998 889 445 404
334 305 270 230 185 168 153 120
72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6030 7823 9535
Size: 274 80 33 51 1443 4053 1793 1712
Cuts at: 9535 96
Size: 138
Fragments arranged by size:
4053 1793 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 6593 6625 9537 9569 2501
Size: 32 4060 32 2912 32 2509
Fragments arranged by size:
4060 2912 2509 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2159 4781 5576 1316
Size: 843 2622 795 5317
Fragments arranged by size:
5317 2622 843 795
XbaI T'CTAG_A
Cuts at: 3481 6913 3481
Size: 3432 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3643 4807 5017 5038 5902 3643
Size: 1164 210 21 864 7318
Fragments arranged by size:
7318 1164 864 210 21
XhoI C'TCGA_G
Cuts at: 1943 2779 5631 1943
Size: 836 2852 5889
Fragments arranged by size:
5889 2852 836
XmaI C'CCGG_G
Cuts at: 1870 2107 5604 6628 6902 1870
Size: 237 3497 1024 274 4545
Fragments arranged by size:
4545 3497 1024 274 237
XmnI GAAnn'nnTTC
Cuts at: 7706 7706
Size: 9577
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5646 7385 276
Size: 53 83 186 936 4112 1739 2468
Fragments arranged by size:
4112 2468 1739 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BfrBI BsiWI BstZ17I NruI NsiI PacI
PmlI PsrI RsrII SacII SanDI SrfI SwaI