Restriction map for
pFN21AA0443
(Circular) (Six-base) MAPSORT of: pFN21AA0443.seq Check: 8663 from: 1 to: 8890
pFN21AA0443 8890 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6700 278
Size: 53 83 186 936 5164 2468
Fragments arranged by size:
5164 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2589 3283 4663 6233 1937
Size: 652 694 1380 1570 4594
Fragments arranged by size:
4594 1570 1380 694 652
Acc65I G'GTAC_C
Cuts at: 6211 6211
Size: 8890
AclI AA'CG_TT
Cuts at: 1563 7017 7390 1563
Size: 5454 373 3063
Fragments arranged by size:
5454 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3102 3348 3747 4254 4533 4668 4728 4950
Size: 1470 246 399 507 279 135 60 222
Cuts at: 4950 5294 5484 5973 6192 6953 8337 8605 1632
Size: 344 190 489 219 761 1384 268 1917
Fragments arranged by size:
1917 1470 1384 761 507 489 399 344
279 268 246 222 219 190 135 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8693
AflIII A'CryG_T
Cuts at: 1203 5090 7789 8766 1203
Size: 3887 2699 977 1327
Fragments arranged by size:
3887 2699 1327 977
AgeI A'CCGG_T
Cuts at: 7721 7721
Size: 8890
AhdI GACnn_n'nnGTC
Cuts at: 7619 7619
Size: 8890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4789 1184
Size: 3605 5285
AlwNI CAG_nnn'CTG
Cuts at: 2441 3725 4007 4322 4388 4706 5414 6633 8205
Size: 1284 282 315 66 318 708 1219 1572
Cuts at: 8205 2441
Size: 3126
Fragments arranged by size:
3126 1572 1284 1219 708 318 315 282
66
ApaI G_GGCC'C
Cuts at: 2829 2829
Size: 8890
ApaLI G'TGCA_C
Cuts at: 6947 8103 6947
Size: 1156 7734
ApoI r'AATT_y
Cuts at: 1493 2639 2951 5376 5499 5908 5966 6125 6199
Size: 1146 312 2425 123 409 58 159 74
Cuts at: 6199 6425 6577 8686 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2425 2109 1697 1146 409 312 226 159
152 123 74 58
AseI AT'TA_AT
Cuts at: 160 1032 5489 7444 160
Size: 872 4457 1955 1606
Fragments arranged by size:
4457 1955 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8890
AvaI C'yCGr_G
Cuts at: 1870 1943 3171 5568 5634 6215 1870
Size: 73 1228 2397 66 581 4545
Fragments arranged by size:
4545 2397 1228 581 73 66
AvrII C'CTAG_G
Cuts at: 2537 2537
Size: 8890
BamHI G'GATC_C
Cuts at: 5506 6220 6587 5506
Size: 714 367 7809
Fragments arranged by size:
7809 714 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 6211 7666 618
Size: 325 217 627 243 4181 1455 1842
Fragments arranged by size:
4181 1842 1455 627 325 243 217
BanII G_rGCy'C
Cuts at: 1391 2293 2739 2829 3837 6209 1391
Size: 902 446 90 1008 2372 4072
Fragments arranged by size:
4072 2372 1008 902 446 90
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2916 4410 928
Size: 971 1017 1494 5408
Fragments arranged by size:
5408 1494 1017 971
BbvCI CC'TCA_GC
Cuts at: 3447 3540 3822 4008 4323 3447
Size: 93 282 186 315 8014
Fragments arranged by size:
8014 315 282 186 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3003 3037 7081 7115 1903
Size: 34 1066 34 4044 34 3678
Fragments arranged by size:
4044 3678 1066 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4071 4792 6782 7992 4071
Size: 721 1990 1210 4969
Fragments arranged by size:
4969 1990 1210 721
BclI T'GATC_A
Cuts at: 1548 3114 5317 1548
Size: 1566 2203 5121
Fragments arranged by size:
5121 2203 1566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7501 136
Size: 107 122 71 631 605 5829 1525
Fragments arranged by size:
5829 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8885 8885
Size: 8890
BlpI GC'TnA_GC
Cuts at: 2292 6307 2292
Size: 4015 4875
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2227 2829 6951 8107 1790
Size: 245 192 602 4122 1156 2573
Fragments arranged by size:
4122 2573 1156 602 245 192
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 3360 3450
Size: 416 53 83 399 24 602 1341 90
Cuts at: 3450 6621 7579 442
Size: 3171 958 1753
Fragments arranged by size:
3171 1753 1341 958 602 416 399 90
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3360 4415 1056
Size: 2304 1055 5531
Fragments arranged by size:
5531 2304 1055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 3472 2353
Size: 1119 7771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2205 2635 3693 3929 4230 4380 7550 1239
Size: 966 430 1058 236 301 150 3170 2579
Fragments arranged by size:
3170 2579 1058 966 430 301 236 150
Bpu10I CC'TnA_GC
Cuts at: 3447 3540 3822 4008 4323 6132 3447
Size: 93 282 186 315 1809 6205
Fragments arranged by size:
6205 1809 315 282 186 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7015 7880 8178 7015
Size: 865 298 7727
Fragments arranged by size:
7727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2504 3542 4007 4235 4457 7553 882
Size: 617 1005 1038 465 228 222 3096 2219
Fragments arranged by size:
3096 2219 1038 1005 617 465 228 222
BsaAI yAC'GTr
Cuts at: 493 1204 5091 6681 493
Size: 711 3887 1590 2702
Fragments arranged by size:
3887 2702 1590 711
BsaBI GATnn'nnATC
Cuts at: 8884 8884
Size: 8890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6697 7079 7714 275
Size: 53 83 186 936 5164 382 635 1451
Fragments arranged by size:
5164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4564 7323 7721 7995 8142 8553 4564
Size: 2759 398 274 147 411 4901
Fragments arranged by size:
4901 2759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2613 2643 2912 2942 3284
Size: 30 165 30 1210 30 269 30 342
Cuts at: 3284 3314 3666 3696 1178
Size: 30 352 30 6372
Fragments arranged by size:
6372 1210 352 342 269 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 3218 3839 3842 3845 4025 4112 4253
Size: 1336 696 621 3 3 180 87 141
Cuts at: 4253 4340 4625 5156 1186
Size: 87 285 531 4920
Fragments arranged by size:
4920 1336 696 621 531 285 180 141
87 87 3 3
BseYI C'CCAG_C
Cuts at: 2972 4481 8093 8589 2972
Size: 1509 3612 496 3273
Fragments arranged by size:
3612 3273 1509 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 1163
Size: 573 496 7821
Fragments arranged by size:
7821 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6340 7101 8129 1540
Size: 275 186 4339 761 1028 2301
Fragments arranged by size:
4339 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 6951 7036 8107 2739
Size: 4212 85 1071 3522
Fragments arranged by size:
4212 3522 1071 85
BsmI GAATG_Cn'
Cuts at: 6107 6401 6494 6107
Size: 294 93 8503
Fragments arranged by size:
8503 294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2227 2293 2739 2829 3837 6209
Size: 399 245 192 66 446 90 1008 2372
Cuts at: 6209 6951 7036 8107 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2372 2174 1071 1008 742 446 399 245
192 90 85 66
BspEI T'CCGG_A
Cuts at: 4564 8553 4564
Size: 3989 4901
BspHI T'CATG_A
Cuts at: 5518 5928 6779 8509 5518
Size: 410 851 1730 5899
Fragments arranged by size:
5899 1730 851 410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2838 5073 6245 844
Size: 1994 2235 1172 3489
Fragments arranged by size:
3489 2235 1994 1172
BsrBI CCG'CTC
Cuts at: 1394 1823 6777 1394
Size: 429 4954 3507
Fragments arranged by size:
4954 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3147 5544 5875 7385 7559 65
Size: 1183 1899 2397 331 1510 174 1396
Fragments arranged by size:
2397 1899 1510 1396 1183 331 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4067 7534 7721 1700
Size: 110 2257 3467 187 2869
Fragments arranged by size:
3467 2869 2257 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8890
BssSI C'ACGA_G
Cuts at: 6950 7962 6950
Size: 1012 7878
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8890
BstBI TT'CG_AA
Cuts at: 7694 7694
Size: 8890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8890
BstXI CCAn_nnnn'nTGG
Cuts at: 2686 5269 2686
Size: 2583 6307
BstYI r'GATC_y
Cuts at: 1972 2675 2836 2897 4673 5506 6220 6587 6972
Size: 703 161 61 1776 833 714 367 385
Cuts at: 6972 6989 8441 8527 8539 8885 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1776 1452 833 714 703 385 367
346 161 86 61 17 12
BstZ17I GTA'TAC
Cuts at: 4664 4664
Size: 8890
BtgI C'CryG_G
Cuts at: 513 1065 5171 513
Size: 552 4106 4232
Fragments arranged by size:
4232 4106 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8151
Fragments arranged by size:
8151 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 2631 4578 5964 6406 7200 7220 2220
Size: 411 1947 1386 442 794 20 3890
Fragments arranged by size:
3890 1947 1386 794 442 411 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2196 2231 529
Size: 35 1632 35 7188
Fragments arranged by size:
7188 1632 35 35
DraI TTT'AAA
Cuts at: 6194 6194
Size: 8890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7897 809
Size: 507 6581 1802
Fragments arranged by size:
6581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2688 3585 4866
Size: 54 1425 50 275 18 858 897 1281
Cuts at: 4866 6337 7226 8855 8
Size: 1471 889 1629 43
Fragments arranged by size:
1629 1471 1425 1281 897 889 858 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6337 1537
Size: 275 4525 4090
Fragments arranged by size:
4525 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 3147 3327 3516 3540 3639 3702 3726
Size: 705 1089 180 189 24 99 63 24
Cuts at: 3726 3915 3948 4098 4182 4230 4299 4359 4455
Size: 189 33 150 84 48 69 60 96
Cuts at: 4455 4468 4509 4548 4704 4797 5460 5985 6820
Size: 13 41 39 156 93 663 525 835
Cuts at: 6820 1353
Size: 3423
Fragments arranged by size:
3423 1089 835 705 663 525 189 189
180 156 150 99 96 93 84 69
63 60 48 41 39 33 24 24
13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7477 7851 7997 387
Size: 1064 138 5888 374 146 1280
Fragments arranged by size:
5888 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2737 2737
Size: 8890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2635 3102 3348 3693 3747 3929
Size: 393 573 430 467 246 345 54 182
Cuts at: 3929 4230 4254 4380 4533 4668 4728 4950 5294
Size: 301 24 126 153 135 60 222 344
Cuts at: 5294 5484 5973 6192 6953 7550 8337 8605 1239
Size: 190 489 219 761 597 787 268 1524
Fragments arranged by size:
1524 787 761 597 573 489 467 430
393 345 344 301 268 246 222 219
190 182 153 135 126 60 54 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 3103 2411
Size: 692 8198
EcoO109I rG'GnC_Cy
Cuts at: 2127 2523 2718 3222 3747 3846 4167 4440 4536
Size: 396 195 504 525 99 321 273 96
Cuts at: 4536 4632 4728 5013 2127
Size: 96 96 285 6004
Fragments arranged by size:
6004 525 504 396 321 285 273 195
99 96 96 96
EcoRI G'AATT_C
Cuts at: 6199 6577 6199
Size: 378 8512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4048 5498 4048
Size: 1450 7440
FspI TGC'GCA
Cuts at: 7396 7396
Size: 8890
HaeII r_GCGC'y
Cuts at: 1678 8037 1678
Size: 6359 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2275 2307 2613
Size: 32 135 32 437 32 234 32 306
Cuts at: 2613 2645 2756 2788 3284 3316 4469 4501 5213
Size: 32 111 32 496 32 1153 32 712
Cuts at: 5213 5245 5285 5317 5408 5440 5776 5808 7531
Size: 32 40 32 91 32 336 32 1723
Cuts at: 7531 7563 7605 7637 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1723 1153 712 496 437 336 306
234 135 111 91 42 40 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3529 3892 6234 6480 669
Size: 988 281 1591 363 2342 246 3079
Fragments arranged by size:
3079 2342 1591 988 363 281 246
HindIII A'AGCT_T
Cuts at: 748 3018 3870 748
Size: 2270 852 5768
Fragments arranged by size:
5768 2270 852
HpaI GTT'AAC
Cuts at: 6480 6480
Size: 8890
KpnI G_GTAC'C
Cuts at: 6215 6215
Size: 8890
MfeI C'AATT_G
Cuts at: 6489 6489
Size: 8890
MluI A'CGCG_T
Cuts at: 8766 8766
Size: 8890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4061 8004 8188 1305
Size: 2756 3943 184 2007
Fragments arranged by size:
3943 2756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 4868 10
Size: 54 1425 343 858 2178 4032
Fragments arranged by size:
4032 2178 1425 858 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 5713 6848 7207 7366 518
Size: 648 1518 3029 1135 359 159 2042
Fragments arranged by size:
3029 2042 1518 1135 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3476 3615 4385 4418 5767 6306 6983 8131
Size: 1870 139 770 33 1349 539 677 1148
Cuts at: 8131 8376 1606
Size: 245 2120
Fragments arranged by size:
2120 1870 1349 1148 770 677 539 245
139 33
NcoI C'CATG_G
Cuts at: 513 1065 5171 513
Size: 552 4106 4232
Fragments arranged by size:
4232 4106 552
NdeI CA'TA_TG
Cuts at: 387 5714 387
Size: 5327 3563
NheI G'CTAG_C
Cuts at: 1052 3356 4411 1052
Size: 2304 1055 5531
Fragments arranged by size:
5531 2304 1055
NotI GC'GGCC_GC
Cuts at: 6337 6337
Size: 8890
NspI r_CATG'y
Cuts at: 1132 1229 6248 7793 1132
Size: 97 5019 1545 2229
Fragments arranged by size:
5019 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2829 1348
Size: 1481 7409
PciI A'CATG_T
Cuts at: 7789 7789
Size: 8890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2817 3941 1108
Size: 1709 1124 6057
Fragments arranged by size:
6057 1709 1124
PfoI T'CCnGG_A
Cuts at: 3623 4976 5370 3623
Size: 1353 394 7143
Fragments arranged by size:
7143 1353 394
PmeI GTTT'AAAC
Cuts at: 6194 6194
Size: 8890
PmlI CAC'GTG
Cuts at: 5091 5091
Size: 8890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6959 6991 8472 8504 4808
Size: 32 2119 32 1481 32 5194
Fragments arranged by size:
5194 2119 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2127 2523 2718 3222 3747 3846 4167 4440 4536
Size: 396 195 504 525 99 321 273 96
Cuts at: 4536 4632 4728 5013 2127
Size: 96 96 285 6004
Fragments arranged by size:
6004 525 504 396 321 285 273 195
99 96 96 96
PshAI GACnn'nnGTC
Cuts at: 1613 3980 1613
Size: 2367 6523
PsiI TTA'TAA
Cuts at: 6460 6460
Size: 8890
PspOMI G'GGCC_C
Cuts at: 2825 2825
Size: 8890
PstI C_TGCA'G
Cuts at: 830 6242 830
Size: 5412 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8890
PvuII CAG'CTG
Cuts at: 3476 3615 4385 4418 5767 3476
Size: 139 770 33 1349 6599
Fragments arranged by size:
6599 1349 770 139 33
SacI G_AGCT'C
Cuts at: 2739 2739
Size: 8890
SalI G'TCGA_C
Cuts at: 1936 6232 1936
Size: 4296 4594
SbfI CC_TGCA'GG
Cuts at: 6242 6242
Size: 8890
ScaI AGT'ACT
Cuts at: 2374 7138 8850 2374
Size: 4764 1712 2414
Fragments arranged by size:
4764 2414 1712
SexAI A'CCwGG_T
Cuts at: 2816 3224 2816
Size: 408 8482
SfcI C'TryA_G
Cuts at: 826 1045 3045 3237 4182 5359 5862 6238 7373
Size: 219 2000 192 945 1177 503 376 1135
Cuts at: 7373 8054 8245 826
Size: 681 191 1471
Fragments arranged by size:
2000 1471 1177 1135 945 681 503 376
219 192 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8890
SmaI CCC'GGG
Cuts at: 1872 6217 1872
Size: 4345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6994 7895 8157 820
Size: 197 926 5051 901 262 1553
Fragments arranged by size:
5051 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6681 493
Size: 6188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8890
SphI G_CATG'C
Cuts at: 1132 6248 1132
Size: 5116 3774
SspI AAT'ATT
Cuts at: 5 52 3741 6007 6027 6037 6088 6814 5
Size: 47 3689 2266 20 10 51 726 2081
Fragments arranged by size:
3689 2266 2081 726 51 47 20 10
StuI AGG'CCT
Cuts at: 1517 2542 5128 5976 1517
Size: 1025 2586 848 4431
Fragments arranged by size:
4431 2586 1025 848
StyI C'CwwG_G
Cuts at: 513 1065 2118 2172 2244 2537 3165 3374 5171
Size: 552 1053 54 72 293 628 209 1797
Cuts at: 5171 5397 6329 513
Size: 226 932 3074
Fragments arranged by size:
3074 1797 1053 932 628 552 293 226
209 72 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 6917 6934 7087 7272 7717 325
Size: 1255 627 4710 17 153 185 445 1498
Fragments arranged by size:
4710 1498 1255 627 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2372 5405 7136
Size: 274 80 33 51 1443 395 3033 1731
Cuts at: 7136 8848 96
Size: 1712 138
Fragments arranged by size:
3033 1731 1712 1443 395 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8850 8882 8850
Size: 32 8858
Tth111I GACn'n_nGTC
Cuts at: 1316 3631 4112 1316
Size: 2315 481 6094
Fragments arranged by size:
6094 2315 481
XbaI T'CTAG_A
Cuts at: 4832 6226 4832
Size: 1394 7496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2144 3286 2144
Size: 1142 7748
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8890
XmaI C'CCGG_G
Cuts at: 1870 6215 1870
Size: 4345 4545
XmnI GAAnn'nnTTC
Cuts at: 5499 5554 7019 5499
Size: 55 1465 7370
Fragments arranged by size:
7370 1465 55
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6698 276
Size: 53 83 186 936 5164 2468
Fragments arranged by size:
5164 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI Bsu36I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI