Restriction map for
pFN21AA0425
(Circular) (Six-base) MAPSORT of: pFN21AA0425.seq Check: 1328 from: 1 to: 8377
pFN21AA0425 8377 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2170 2741 2170
Size: 571 7806
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6187 278
Size: 53 83 186 936 4651 2468
Fragments arranged by size:
4651 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2111 2827 5720 1937
Size: 174 716 2893 4594
Fragments arranged by size:
4594 2893 716 174
Acc65I G'GTAC_C
Cuts at: 5698 5698
Size: 8377
AclI AA'CG_TT
Cuts at: 1563 6504 6877 1563
Size: 4941 373 3063
Fragments arranged by size:
4941 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2525 2886 3630 4865 5294 5670 6440
Size: 375 518 361 744 1235 429 376 770
Cuts at: 6440 7824 8092 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1235 770 744 518 429 376
375 361 268
AfeI AGC'GCT
Cuts at: 5522 5522
Size: 8377
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8180
AflIII A'CryG_T
Cuts at: 1203 4653 5097 5597 7276 8253 1203
Size: 3450 444 500 1679 977 1327
Fragments arranged by size:
3450 1679 1327 977 500 444
AgeI A'CCGG_T
Cuts at: 7208 7208
Size: 8377
AhdI GACnn_n'nnGTC
Cuts at: 4858 7106 4858
Size: 2248 6129
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8377
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2139 2171 4041 4073 2139
Size: 32 1870 32 6443
Fragments arranged by size:
6443 1870 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3272 4444 4984 6120 7692 3272
Size: 1172 540 1136 1572 3957
Fragments arranged by size:
3957 1572 1172 1136 540
ApaLI G'TGCA_C
Cuts at: 6434 7590 6434
Size: 1156 7221
ApoI r'AATT_y
Cuts at: 1493 2869 2896 3739 4050 4971 5686 5912 6064
Size: 1376 27 843 311 921 715 226 152
Cuts at: 6064 8173 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1376 921 843 715 311 226
152 27
AseI AT'TA_AT
Cuts at: 160 1032 6931 160
Size: 872 5899 1606
Fragments arranged by size:
5899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8377
AvaI C'yCGr_G
Cuts at: 1870 1943 5702 1870
Size: 73 3759 4545
Fragments arranged by size:
4545 3759 73
BamHI G'GATC_C
Cuts at: 5707 6074 5707
Size: 367 8010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3976 5698 7153 618
Size: 325 217 627 2189 1722 1455 1842
Fragments arranged by size:
2189 1842 1722 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4593 5025 5696 1391
Size: 3202 432 671 4072
Fragments arranged by size:
4072 3202 671 432
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2693 3951 4134 4220 4857 5658 928
Size: 971 794 1258 183 86 637 801 3647
Fragments arranged by size:
3647 1258 971 801 794 637 183 86
BbvCI CC'TCA_GC
Cuts at: 2650 2785 2650
Size: 135 8242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6568 6602 1903
Size: 34 4631 34 3678
Fragments arranged by size:
4631 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6269 7479 6269
Size: 1210 7167
BclI T'GATC_A
Cuts at: 1548 2238 1548
Size: 690 7687
BfrBI ATG'CAT
Cuts at: 3438 3438
Size: 8377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6988 136
Size: 107 122 71 631 605 5316 1525
Fragments arranged by size:
5316 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8372 8372
Size: 8377
BlpI GC'TnA_GC
Cuts at: 3726 5794 3726
Size: 2068 6309
Bme1580I G_kGCm'C
Cuts at: 1790 2248 2913 3718 6438 7594 1790
Size: 458 665 805 2720 1156 2573
Fragments arranged by size:
2720 2573 1156 805 665 458
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2242 3040 4690
Size: 416 53 83 399 24 825 798 1650
Cuts at: 4690 5432 6108 7066 442
Size: 742 676 958 1753
Fragments arranged by size:
1753 1650 958 825 798 742 676 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 3014 3666 4020 7037 1239
Size: 821 954 652 354 3017 2579
Fragments arranged by size:
3017 2579 954 821 652 354
Bpu10I CC'TnA_GC
Cuts at: 2650 2785 5517 2650
Size: 135 2732 5510
Fragments arranged by size:
5510 2732 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4196 4767 4785 6502 7367 7665 4196
Size: 571 18 1717 865 298 4908
Fragments arranged by size:
4908 1717 865 571 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3032 4912 7040 882
Size: 617 1533 1880 2128 2219
Fragments arranged by size:
2219 2128 1880 1533 617
BsaAI yAC'GTr
Cuts at: 493 1204 5600 6168 493
Size: 711 4396 568 2702
Fragments arranged by size:
4396 2702 711 568
BsaBI GATnn'nnATC
Cuts at: 8371 8371
Size: 8377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6184 6566 7201 275
Size: 53 83 186 936 4651 382 635 1451
Fragments arranged by size:
4651 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6810 7208 7482 7629 8040 6810
Size: 398 274 147 411 7147
Fragments arranged by size:
7147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3396 3426 1178
Size: 30 165 30 1993 30 6129
Fragments arranged by size:
6129 1993 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2169 2985 1186
Size: 983 816 6578
Fragments arranged by size:
6578 983 816
BseYI C'CCAG_C
Cuts at: 3051 3333 4344 5143 7580 8076 3051
Size: 282 1011 799 2437 496 3352
Fragments arranged by size:
3352 2437 1011 799 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3376 3783 4849 1163
Size: 573 1640 407 1066 4691
Fragments arranged by size:
4691 1640 1066 573 407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5827 6588 7616 1540
Size: 275 186 3826 761 1028 2301
Fragments arranged by size:
3826 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5229 6438 6523 7594 5229
Size: 1209 85 1071 6012
Fragments arranged by size:
6012 1209 1071 85
BsmI GAATG_Cn'
Cuts at: 2386 3440 3938 5888 5981 2386
Size: 1054 498 1950 93 4782
Fragments arranged by size:
4782 1950 1054 498 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3041 5251 3041
Size: 2210 6167
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2248 2913 3718 4593 5025 5229 5696
Size: 399 458 665 805 875 432 204 467
Cuts at: 5696 6438 6523 7594 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 875 805 742 665 467 458
432 399 204 85
BspEI T'CCGG_A
Cuts at: 8040 8040
Size: 8377
BspHI T'CATG_A
Cuts at: 6266 7996 6266
Size: 1730 6647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2170 2741 3561 5732 844
Size: 1326 571 820 2171 3489
Fragments arranged by size:
3489 2171 1326 820 571
BsrBI CCG'CTC
Cuts at: 1394 1823 4833 6264 1394
Size: 429 3010 1431 3507
Fragments arranged by size:
3507 3010 1431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3219 6872 7046 65
Size: 1183 1971 3653 174 1396
Fragments arranged by size:
3653 1971 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7021 7208 1700
Size: 110 5211 187 2869
Fragments arranged by size:
5211 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2755 96
Size: 2659 5718
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8377
BssSI C'ACGA_G
Cuts at: 6437 7449 6437
Size: 1012 7365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2838 794
Size: 2044 6333
BstBI TT'CG_AA
Cuts at: 7181 7181
Size: 8377
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8377
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 3059 4344 2680
Size: 379 1285 6713
Fragments arranged by size:
6713 1285 379
BstYI r'GATC_y
Cuts at: 1972 2645 4717 5707 6074 6459 6476 7928 8014
Size: 673 2072 990 367 385 17 1452 86
Cuts at: 8014 8026 8372 1972
Size: 12 346 1977
Fragments arranged by size:
2072 1977 1452 990 673 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2828 2828
Size: 8377
Bsu36I CC'TnA_GG
Cuts at: 4771 4771
Size: 8377
BtgI C'CryG_G
Cuts at: 513 1065 3630 513
Size: 552 2565 5260
Fragments arranged by size:
5260 2565 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7638
Fragments arranged by size:
7638 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 5893 6687 6707 2440
Size: 3453 794 20 4110
Fragments arranged by size:
4110 3453 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8342
DraI TTT'AAA
Cuts at: 2223 5172 5681 2223
Size: 2949 509 4919
Fragments arranged by size:
4919 2949 509
DraIII CAC_nnn'GTG
Cuts at: 2136 2624 3074 2136
Size: 488 450 7439
Fragments arranged by size:
7439 488 450
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7384 809
Size: 507 6068 1802
Fragments arranged by size:
6068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3471 5824 6713
Size: 54 1425 50 275 18 1641 2353 889
Cuts at: 6713 8342 8
Size: 1629 43
Fragments arranged by size:
2353 1641 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5824 1537
Size: 275 4012 4090
Fragments arranged by size:
4090 4012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3411 4017 4200 4347 4552 4701 5112 6307
Size: 2058 606 183 147 205 149 411 1195
Cuts at: 6307 1353
Size: 3423
Fragments arranged by size:
3423 2058 1195 606 411 205 183 149
147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6964 7338 7484 387
Size: 1064 138 5375 374 146 1280
Fragments arranged by size:
5375 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2060 2525 2886 3014 3630 3666
Size: 393 375 53 465 361 128 616 36
Cuts at: 3666 4020 4865 5294 5670 6440 7037 7824 8092
Size: 354 845 429 376 770 597 787 268
Cuts at: 8092 1239
Size: 1524
Fragments arranged by size:
1524 845 787 770 616 597 465 429
393 376 375 361 354 268 128 53
36
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 2078
Size: 8377
EcoO109I rG'GnC_Cy
Cuts at: 3653 4441 3653
Size: 788 7589
EcoRI G'AATT_C
Cuts at: 2896 3739 4050 4971 5686 6064 2896
Size: 843 311 921 715 378 5209
Fragments arranged by size:
5209 921 843 715 378 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4160 5257 4160
Size: 1097 7280
FspI TGC'GCA
Cuts at: 6883 6883
Size: 8377
HaeII r_GCGC'y
Cuts at: 1678 5151 5524 7524 1678
Size: 3473 373 2000 2531
Fragments arranged by size:
3473 2531 2000 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 4207 4239 4301
Size: 32 135 32 968 32 1635 32 62
Cuts at: 4301 4313 4333 4345 4445 4477 4482 4514 4844
Size: 12 20 12 100 32 5 32 330
Cuts at: 4844 4876 5254 5281 5286 5313 5424 5456 7018
Size: 32 378 27 5 27 111 32 1562
Cuts at: 7018 7050 7092 7124 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1635 1562 968 378 330 135 111
100 62 42 32 32 32 32 32
32 32 32 32 32 27 27 20
12 12 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2301 3095 5595 5721 5967 669
Size: 988 281 363 794 2500 126 246 3079
Fragments arranged by size:
3079 2500 988 794 363 281 246 126
HindIII A'AGCT_T
Cuts at: 748 3864 4142 5234 748
Size: 3116 278 1092 3891
Fragments arranged by size:
3891 3116 1092 278
HpaI GTT'AAC
Cuts at: 5595 5967 5595
Size: 372 8005
KpnI G_GTAC'C
Cuts at: 5702 5702
Size: 8377
MfeI C'AATT_G
Cuts at: 5976 5976
Size: 8377
MluI A'CGCG_T
Cuts at: 8253 8253
Size: 8377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3794 5230 5467 7491 7675 1305
Size: 2489 1436 237 2024 184 2007
Fragments arranged by size:
2489 2024 2007 1436 237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3473 10
Size: 54 1425 343 1641 4914
Fragments arranged by size:
4914 1641 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4533 5431 6335 6694 6853 518
Size: 648 3367 898 904 359 159 2042
Fragments arranged by size:
3367 2042 904 898 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2850 3023 5143 5793 6470 7618 7863
Size: 433 811 173 2120 650 677 1148 245
Cuts at: 7863 1606
Size: 2120
Fragments arranged by size:
2120 2120 1148 811 677 650 433 245
173
NcoI C'CATG_G
Cuts at: 513 1065 3630 513
Size: 552 2565 5260
Fragments arranged by size:
5260 2565 552
NdeI CA'TA_TG
Cuts at: 387 2305 387
Size: 1918 6459
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8377
NotI GC'GGCC_GC
Cuts at: 5824 5824
Size: 8377
NsiI A_TGCA'T
Cuts at: 3440 3440
Size: 8377
NspI r_CATG'y
Cuts at: 1132 1229 2371 2539 3059 3442 4657 5101 5735
Size: 97 1142 168 520 383 1215 444 634
Cuts at: 5735 7280 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1215 1142 634 520 444 383
168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8377
PciI A'CATG_T
Cuts at: 4653 5097 7276 4653
Size: 444 2179 5754
Fragments arranged by size:
5754 2179 444
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2416 1108
Size: 1308 7069
PfoI T'CCnGG_A
Cuts at: 4634 4634
Size: 8377
PmeI GTTT'AAAC
Cuts at: 5681 5681
Size: 8377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4239 4271 6446 6478 7959 7991 4239
Size: 32 2175 32 1481 32 4625
Fragments arranged by size:
4625 2175 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3653 4441 3653
Size: 788 7589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8377
PsiI TTA'TAA
Cuts at: 3312 5947 3312
Size: 2635 5742
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5064 5096 5064
Size: 32 8345
PstI C_TGCA'G
Cuts at: 830 2752 2843 3022 5729 830
Size: 1922 91 179 2707 3478
Fragments arranged by size:
3478 2707 1922 179 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8377
PvuII CAG'CTG
Cuts at: 2039 2850 3023 5143 2039
Size: 811 173 2120 5273
Fragments arranged by size:
5273 2120 811 173
SalI G'TCGA_C
Cuts at: 1936 5719 1936
Size: 3783 4594
SapI GCTCTTCn'nnn_
Cuts at: 4347 4701 5112 4347
Size: 354 411 7612
Fragments arranged by size:
7612 411 354
SbfI CC_TGCA'GG
Cuts at: 5729 5729
Size: 8377
ScaI AGT'ACT
Cuts at: 3805 4780 6625 8337 3805
Size: 975 1845 1712 3845
Fragments arranged by size:
3845 1845 1712 975
SfcI C'TryA_G
Cuts at: 826 1045 2748 2839 2845 3018 3286 3732 4575
Size: 219 1703 91 6 173 268 446 843
Cuts at: 4575 4822 5725 6860 7541 7732 826
Size: 247 903 1135 681 191 1471
Fragments arranged by size:
1703 1471 1135 903 843 681 446 268
247 219 191 173 91 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8377
SmaI CCC'GGG
Cuts at: 1872 5704 1872
Size: 3832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4175 4782 4800 6481 7382 7644
Size: 197 926 2232 607 18 1681 901 262
Cuts at: 7644 820
Size: 1553
Fragments arranged by size:
2232 1681 1553 926 901 607 262 197
18
SnaBI TAC'GTA
Cuts at: 493 6168 493
Size: 5675 2702
SpeI A'CTAG_T
Cuts at: 152 2267 152
Size: 2115 6262
SphI G_CATG'C
Cuts at: 1132 5735 1132
Size: 4603 3774
SspI AAT'ATT
Cuts at: 5 52 3224 3625 6301 5
Size: 47 3172 401 2676 2081
Fragments arranged by size:
3172 2676 2081 401 47
StuI AGG'CCT
Cuts at: 1517 4745 1517
Size: 3228 5149
StyI C'CwwG_G
Cuts at: 513 1065 3630 3845 4288 4701 4966 5816 513
Size: 552 2565 215 443 413 265 850 3074
Fragments arranged by size:
3074 2565 850 552 443 413 265 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6404 6421 6574 6759 7204 325
Size: 1255 4824 17 153 185 445 1498
Fragments arranged by size:
4824 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2755 3803 4295
Size: 274 80 33 51 1443 778 1048 492
Cuts at: 4295 4778 5277 6623 8335 96
Size: 483 499 1346 1712 138
Fragments arranged by size:
1712 1443 1346 1048 778 499 492 483
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3396 3428 5148 5180 8337 8369 3396
Size: 32 1720 32 3157 32 3404
Fragments arranged by size:
3404 3157 1720 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8377
XbaI T'CTAG_A
Cuts at: 4261 5713 4261
Size: 1452 6925
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8377
XmaI C'CCGG_G
Cuts at: 1870 5702 1870
Size: 3832 4545
XmnI GAAnn'nnTTC
Cuts at: 2873 2896 3542 3673 4143 4257 4415 4854 6506
Size: 23 646 131 470 114 158 439 1652
Cuts at: 6506 2873
Size: 4744
Fragments arranged by size:
4744 1652 646 470 439 158 131 114
23
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6185 276
Size: 53 83 186 936 4651 2468
Fragments arranged by size:
4651 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BplI BsiWI EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI