Restriction map for
pFN21AA0419
(Circular) (Six-base) MAPSORT of: pFN21AA0419.seq Check: 8568 from: 1 to: 7678
pFN21AA0419 7678 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5488 278
Size: 53 83 186 936 3952 2468
Fragments arranged by size:
3952 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4441 4897 5021 1937
Size: 2504 456 124 4594
Fragments arranged by size:
4594 2504 456 124
Acc65I G'GTAC_C
Cuts at: 4999 4999
Size: 7678
AclI AA'CG_TT
Cuts at: 1563 5805 6178 1563
Size: 4242 373 3063
Fragments arranged by size:
4242 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 2672 3366 3381 4484 4553 4835 5741
Size: 930 110 694 15 1103 69 282 906
Cuts at: 5741 7125 7393 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1103 930 906 694 282 268
110 69 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7481
AflIII A'CryG_T
Cuts at: 1203 6577 7554 1203
Size: 5374 977 1327
Fragments arranged by size:
5374 1327 977
AgeI A'CCGG_T
Cuts at: 6509 6509
Size: 7678
AhdI GACnn_n'nnGTC
Cuts at: 6407 6407
Size: 7678
AleI CACnn'nnGTG
Cuts at: 1166 3263 1166
Size: 2097 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2960 1184
Size: 1776 5902
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2980 3012 2980
Size: 32 7646
AlwNI CAG_nnn'CTG
Cuts at: 2695 3495 5421 6993 2695
Size: 800 1926 1572 3380
Fragments arranged by size:
3380 1926 1572 800
ApaLI G'TGCA_C
Cuts at: 5735 6891 5735
Size: 1156 6522
ApoI r'AATT_y
Cuts at: 1493 4216 4397 4499 4811 4987 5213 5365 7474
Size: 2723 181 102 312 176 226 152 2109
Cuts at: 7474 1493
Size: 1697
Fragments arranged by size:
2723 2109 1697 312 226 181 176 152
102
AseI AT'TA_AT
Cuts at: 160 1032 6232 160
Size: 872 5200 1606
Fragments arranged by size:
5200 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7678
AvaI C'yCGr_G
Cuts at: 1870 1943 5003 1870
Size: 73 3060 4545
Fragments arranged by size:
4545 3060 73
BamHI G'GATC_C
Cuts at: 5008 5375 5008
Size: 367 7311
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4999 6454 618
Size: 325 217 627 3212 1455 1842
Fragments arranged by size:
3212 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4997 1391
Size: 3606 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2678 3142 4966 928
Size: 971 779 464 1824 3640
Fragments arranged by size:
3640 1824 971 779 464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5869 5903 1903
Size: 34 3932 34 3678
Fragments arranged by size:
3932 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5570 6780 5570
Size: 1210 6468
BclI T'GATC_A
Cuts at: 1548 2305 1548
Size: 757 6921
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6289 136
Size: 107 122 71 631 605 4617 1525
Fragments arranged by size:
4617 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7673 7673
Size: 7678
BlpI GC'TnA_GC
Cuts at: 4020 5095 4020
Size: 1075 6603
Bme1580I G_kGCm'C
Cuts at: 1790 3270 5739 6895 1790
Size: 1480 2469 1156 2573
Fragments arranged by size:
2573 2469 1480 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7678
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5409 6367 442
Size: 416 53 83 399 24 3992 958 1753
Fragments arranged by size:
3992 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2961 1056
Size: 1905 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6338 1239
Size: 5099 2579
Bpu10I CC'TnA_GC
Cuts at: 4697 4697
Size: 7678
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2910 3563 4176 4353 4539 5803 6668 6966 2910
Size: 653 613 177 186 1264 865 298 3622
Fragments arranged by size:
3622 1264 865 653 613 298 186 177
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4371 6341 882
Size: 617 2872 1970 2219
Fragments arranged by size:
2872 2219 1970 617
BsaAI yAC'GTr
Cuts at: 493 1204 5469 493
Size: 711 4265 2702
Fragments arranged by size:
4265 2702 711
BsaBI GATnn'nnATC
Cuts at: 2304 7672 2304
Size: 5368 2310
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5485 5867 6502 275
Size: 53 83 186 936 3952 382 635 1451
Fragments arranged by size:
3952 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6111 6509 6783 6930 7341 6111
Size: 398 274 147 411 6448
Fragments arranged by size:
6448 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4061 4091 1178
Size: 30 165 30 2658 30 4765
Fragments arranged by size:
4765 2658 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 3944 1186
Size: 838 1920 4920
Fragments arranged by size:
4920 1920 838
BseYI C'CCAG_C
Cuts at: 2432 3996 6881 7377 2432
Size: 1564 2885 496 2733
Fragments arranged by size:
2885 2733 1564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5128 5889 6917 1540
Size: 275 186 3127 761 1028 2301
Fragments arranged by size:
3127 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5739 5824 6895 5739
Size: 85 1071 6522
Fragments arranged by size:
6522 1071 85
BsmI GAATG_Cn'
Cuts at: 4803 4876 5189 5282 4803
Size: 73 313 93 7199
Fragments arranged by size:
7199 313 93 73
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3270 4997 5739 5824 6895 1391
Size: 399 1480 1727 742 85 1071 2174
Fragments arranged by size:
2174 1727 1480 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7341 7341
Size: 7678
BspHI T'CATG_A
Cuts at: 4615 4666 5567 7297 4615
Size: 51 901 1730 4996
Fragments arranged by size:
4996 1730 901 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 5033 844
Size: 1362 2827 3489
Fragments arranged by size:
3489 2827 1362
BsrBI CCG'CTC
Cuts at: 1394 1823 5565 1394
Size: 429 3742 3507
Fragments arranged by size:
3742 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2528 3231 4101 6173 6347 65
Size: 1183 1280 703 870 2072 174 1396
Fragments arranged by size:
2072 1396 1280 1183 870 703 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6322 6509 1700
Size: 110 4512 187 2869
Fragments arranged by size:
4512 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7678
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7678
BssSI C'ACGA_G
Cuts at: 2244 4725 5738 6750 2244
Size: 2481 1013 1012 3172
Fragments arranged by size:
3172 2481 1013 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2291 3850 794
Size: 1497 1559 4622
Fragments arranged by size:
4622 1559 1497
BstBI TT'CG_AA
Cuts at: 2966 6482 2966
Size: 3516 4162
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7678
BstYI r'GATC_y
Cuts at: 1972 2638 2668 3875 5008 5375 5760 5777 7229
Size: 666 30 1207 1133 367 385 17 1452
Cuts at: 7229 7315 7327 7673 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1207 1133 666 385 367 346
86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3870 3870
Size: 7678
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6187
Fragments arranged by size:
6187 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3518 487
Size: 683 56 2292 4647
Fragments arranged by size:
4647 2292 683 56
BtsI GCAGTG_nn'
Cuts at: 2141 4140 4422 5194 5988 6008 2141
Size: 1999 282 772 794 20 3811
Fragments arranged by size:
3811 1999 794 772 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7678
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7643
DraI TTT'AAA
Cuts at: 3028 4982 3028
Size: 1954 5724
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6685 809
Size: 507 5369 1802
Fragments arranged by size:
5369 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2992 4935 5125
Size: 54 1425 50 275 18 1162 1943 190
Cuts at: 5125 6014 7643 8
Size: 889 1629 43
Fragments arranged by size:
1943 1629 1425 1162 889 275 190 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5125 1537
Size: 275 3313 4090
Fragments arranged by size:
4090 3313 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2581 3968 4056 4342 5608 1353
Size: 1228 1387 88 286 1266 3423
Fragments arranged by size:
3423 1387 1266 1228 286 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6265 6639 6785 387
Size: 1064 138 4676 374 146 1280
Fragments arranged by size:
4676 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2562 2672 3366 3381 4484 4553 4835
Size: 393 930 110 694 15 1103 69 282
Cuts at: 4835 5741 6338 7125 7393 1239
Size: 906 597 787 268 1524
Fragments arranged by size:
1524 1103 930 906 787 694 597 393
282 268 110 69 15
EcoRI G'AATT_C
Cuts at: 4499 4987 5365 4499
Size: 488 378 6812
Fragments arranged by size:
6812 488 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2615 4448 2615
Size: 1833 5845
FspI TGC'GCA
Cuts at: 6184 6184
Size: 7678
HaeII r_GCGC'y
Cuts at: 1678 6825 1678
Size: 5147 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 6319 6351 6393
Size: 32 135 32 543 32 4172 32 42
Cuts at: 6393 6425 1373
Size: 32 2626
Fragments arranged by size:
4172 2626 543 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2221 2844 3456 3889 5022 5268
Size: 988 281 283 623 612 433 1133 246
Cuts at: 5268 669
Size: 3079
Fragments arranged by size:
3079 1133 988 623 612 433 283 281
246
HindIII A'AGCT_T
Cuts at: 748 2232 2417 2559 748
Size: 1484 185 142 5867
Fragments arranged by size:
5867 1484 185 142
HpaI GTT'AAC
Cuts at: 2221 3456 3889 5268 2221
Size: 1235 433 1379 4631
Fragments arranged by size:
4631 1379 1235 433
KpnI G_GTAC'C
Cuts at: 5003 5003
Size: 7678
MfeI C'AATT_G
Cuts at: 5277 5277
Size: 7678
MluI A'CGCG_T
Cuts at: 7554 7554
Size: 7678
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4098 6792 6976 1305
Size: 2793 2694 184 2007
Fragments arranged by size:
2793 2694 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2994 10
Size: 54 1425 343 1162 4694
Fragments arranged by size:
4694 1425 1162 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2942 3157 3263 3704 5636 5995 6154
Size: 648 1776 215 106 441 1932 359 159
Cuts at: 6154 518
Size: 2042
Fragments arranged by size:
2042 1932 1776 648 441 359 215 159
106
MspA1I CmG'CkG
Cuts at: 1606 4103 4643 4742 5094 5771 6919 7164 1606
Size: 2497 540 99 352 677 1148 245 2120
Fragments arranged by size:
2497 2120 1148 677 540 352 245 99
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6187
Fragments arranged by size:
6187 939 552
NdeI CA'TA_TG
Cuts at: 387 3085 3526 387
Size: 2698 441 4539
Fragments arranged by size:
4539 2698 441
NheI G'CTAG_C
Cuts at: 1052 2957 1052
Size: 1905 5773
NotI GC'GGCC_GC
Cuts at: 5125 5125
Size: 7678
NspI r_CATG'y
Cuts at: 1132 1229 5036 6581 1132
Size: 97 3807 1545 2229
Fragments arranged by size:
3807 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7678
PciI A'CATG_T
Cuts at: 6577 6577
Size: 7678
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3560 1108
Size: 2452 5226
PfoI T'CCnGG_A
Cuts at: 2250 3143 2250
Size: 893 6785
PmeI GTTT'AAAC
Cuts at: 4982 4982
Size: 7678
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5747 5779 7260 7292 5747
Size: 32 1481 32 6133
Fragments arranged by size:
6133 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2246 1613
Size: 633 7045
PsiI TTA'TAA
Cuts at: 3173 5248 3173
Size: 2075 5603
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2006 2038 2006
Size: 32 7646
PstI C_TGCA'G
Cuts at: 830 2413 3611 4417 5030 830
Size: 1583 1198 806 613 3478
Fragments arranged by size:
3478 1583 1198 806 613
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7678
PvuII CAG'CTG
Cuts at: 4643 4742 4643
Size: 99 7579
SalI G'TCGA_C
Cuts at: 1936 5020 1936
Size: 3084 4594
SapI GCTCTTCn'nnn_
Cuts at: 4056 4342 4056
Size: 286 7392
SbfI CC_TGCA'GG
Cuts at: 5030 5030
Size: 7678
ScaI AGT'ACT
Cuts at: 5926 7638 5926
Size: 1712 5966
SfcI C'TryA_G
Cuts at: 826 1045 2409 3607 4413 5026 6161 6842 7033
Size: 219 1364 1198 806 613 1135 681 191
Cuts at: 7033 826
Size: 1471
Fragments arranged by size:
1471 1364 1198 1135 806 681 613 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7678
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7678
SmaI CCC'GGG
Cuts at: 1872 5005 1872
Size: 3133 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2925 3578 4191 4368 4554 5782
Size: 197 926 982 653 613 177 186 1228
Cuts at: 5782 6683 6945 820
Size: 901 262 1553
Fragments arranged by size:
1553 1228 982 926 901 653 613 262
197 186 177
SnaBI TAC'GTA
Cuts at: 493 5469 493
Size: 4976 2702
SpeI A'CTAG_T
Cuts at: 152 3911 4307 152
Size: 3759 396 3523
Fragments arranged by size:
3759 3523 396
SphI G_CATG'C
Cuts at: 1132 5036 1132
Size: 3904 3774
SspI AAT'ATT
Cuts at: 5 52 2539 5602 5
Size: 47 2487 3063 2081
Fragments arranged by size:
3063 2487 2081 47
StuI AGG'CCT
Cuts at: 1517 4339 1517
Size: 2822 4856
StyI C'CwwG_G
Cuts at: 513 1065 2004 2911 4861 5117 513
Size: 552 939 907 1950 256 3074
Fragments arranged by size:
3074 1950 939 907 552 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5705 5722 5875 6060 6505 325
Size: 1255 4125 17 153 185 445 1498
Fragments arranged by size:
4125 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5924 7636 96
Size: 274 80 33 51 1443 3947 1712 138
Fragments arranged by size:
3947 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7638 7670 7638
Size: 32 7646
Tth111I GACn'n_nGTC
Cuts at: 1316 2742 1316
Size: 1426 6252
XbaI T'CTAG_A
Cuts at: 2735 3007 5014 2735
Size: 272 2007 5399
Fragments arranged by size:
5399 2007 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4290 4290
Size: 7678
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7678
XmaI C'CCGG_G
Cuts at: 1870 5003 1870
Size: 3133 4545
XmnI GAAnn'nnTTC
Cuts at: 2562 3139 3913 5807 2562
Size: 577 774 1894 4433
Fragments arranged by size:
4433 1894 774 577
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5486 276
Size: 53 83 186 936 3952 2468
Fragments arranged by size:
3952 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI