Restriction map for
pFN21AA0418
(Circular) (Six-base) MAPSORT of: pFN21AA0418.seq Check: 9556 from: 1 to: 7525
pFN21AA0418 7525 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4018 4336 4018
Size: 318 7207
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5335 278
Size: 53 83 186 936 3799 2468
Fragments arranged by size:
3799 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4868 1937
Size: 2931 4594
Acc65I G'GTAC_C
Cuts at: 4846 4846
Size: 7525
AclI AA'CG_TT
Cuts at: 1563 4824 5652 6025 1563
Size: 3261 828 373 3063
Fragments arranged by size:
3261 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 3234 3599 5588 6972 7240 1632
Size: 773 829 365 1989 1384 268 1917
Fragments arranged by size:
1989 1917 1384 829 773 365 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7328
AflIII A'CryG_T
Cuts at: 1203 6424 7401 1203
Size: 5221 977 1327
Fragments arranged by size:
5221 1327 977
AgeI A'CCGG_T
Cuts at: 6356 6356
Size: 7525
AhdI GACnn_n'nnGTC
Cuts at: 3322 3669 6254 3322
Size: 347 2585 4593
Fragments arranged by size:
4593 2585 347
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7525
AlwNI CAG_nnn'CTG
Cuts at: 3131 4033 4765 5268 6840 3131
Size: 902 732 503 1572 3816
Fragments arranged by size:
3816 1572 902 732 503
ApaI G_GGCC'C
Cuts at: 2677 2917 3038 3229 4093 2677
Size: 240 121 191 864 6109
Fragments arranged by size:
6109 864 240 191 121
ApaLI G'TGCA_C
Cuts at: 5582 6738 5582
Size: 1156 6369
ApoI r'AATT_y
Cuts at: 1493 2798 4834 5060 5212 7321 1493
Size: 1305 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 1305 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6079 160
Size: 872 5047 1606
Fragments arranged by size:
5047 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7525
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 3675 4286 4850 1870
Size: 73 244 1488 611 564 4545
Fragments arranged by size:
4545 1488 611 564 244 73
BamHI G'GATC_C
Cuts at: 4855 5222 4855
Size: 367 7158
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2368 2989 3762 4795 4846
Size: 325 217 627 581 621 773 1033 51
Cuts at: 4846 6301 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1033 773 627 621 581 325
217 51
BanII G_rGCy'C
Cuts at: 1391 2642 2677 2763 2917 3038 3150 3229 3801
Size: 1251 35 86 154 121 112 79 572
Cuts at: 3801 3809 4093 4844 1391
Size: 8 284 751 4072
Fragments arranged by size:
4072 1251 751 572 284 154 121 112
86 79 35 8
BbeI G_GCGC'C
Cuts at: 2372 3766 2372
Size: 1394 6131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3240 928
Size: 971 1341 5213
Fragments arranged by size:
5213 1341 971
BbvCI CC'TCA_GC
Cuts at: 2667 3484 2667
Size: 817 6708
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4514 4548 5716 5750 1903
Size: 34 2577 34 1168 34 3678
Fragments arranged by size:
3678 2577 1168 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5417 6627 5417
Size: 1210 6315
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7525
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3196 3851 4455
Size: 107 122 71 631 605 1524 655 604
Cuts at: 4455 4505 4529 6136 136
Size: 50 24 1607 1525
Fragments arranged by size:
1607 1525 1524 655 631 605 604 122
107 71 50 24
BglII A'GATC_T
Cuts at: 7520 7520
Size: 7525
BlpI GC'TnA_GC
Cuts at: 2063 3137 4942 2063
Size: 1074 1805 4646
Fragments arranged by size:
4646 1805 1074
Bme1580I G_kGCm'C
Cuts at: 1790 2677 2917 2994 3038 3229 4093 4164 5586
Size: 887 240 77 44 191 864 71 1422
Cuts at: 5586 6742 1790
Size: 1156 2573
Fragments arranged by size:
2573 1422 1156 887 864 240 191 77
71 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7525
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2429 3852 4357
Size: 416 53 83 399 24 1012 1423 505
Cuts at: 4357 5256 6214 442
Size: 899 958 1753
Fragments arranged by size:
1753 1423 1012 958 899 505 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2189 2241 2608 2628 4055 4100 4223
Size: 815 135 52 367 20 1427 45 123
Cuts at: 4223 4299 4748 6185 1239
Size: 76 449 1437 2579
Fragments arranged by size:
2579 1437 1427 815 449 367 135 123
76 52 45 20
Bpu10I CC'TnA_GC
Cuts at: 2667 2954 3132 3484 2667
Size: 287 178 352 6708
Fragments arranged by size:
6708 352 287 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3062 3422 3785 4832 5650 6515 6813 3062
Size: 360 363 1047 818 865 298 3774
Fragments arranged by size:
3774 1047 865 818 363 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3269 3766 6188 882
Size: 617 1770 497 2422 2219
Fragments arranged by size:
2422 2219 1770 617 497
BsaAI yAC'GTr
Cuts at: 493 1204 2023 2887 5316 493
Size: 711 819 864 2429 2702
Fragments arranged by size:
2702 2429 864 819 711
BsaBI GATnn'nnATC
Cuts at: 3746 7519 3746
Size: 3773 3752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2295 2369 2489 3763
Size: 53 83 186 936 762 74 120 1274
Cuts at: 3763 4458 5332 5714 6349 275
Size: 695 874 382 635 1451
Fragments arranged by size:
1451 1274 936 874 762 695 635 382
186 120 83 74 53
BsaWI w'CCGG_w
Cuts at: 2313 5958 6356 6630 6777 7188 2313
Size: 3645 398 274 147 411 2650
Fragments arranged by size:
3645 2650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2692 2722 3080 3110 3338
Size: 30 165 30 1289 30 358 30 228
Cuts at: 3338 3368 4037 4067 4697 4727 1178
Size: 30 669 30 630 30 3976
Fragments arranged by size:
3976 1289 669 630 358 228 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2243 3044 3101 3158 3287 3328 3358
Size: 967 90 801 57 57 129 41 30
Cuts at: 3358 3472 3520 3523 3532 3535 3538 4462 4700
Size: 114 48 3 9 3 3 924 238
Cuts at: 4700 1186
Size: 4011
Fragments arranged by size:
4011 967 924 801 238 129 114 90
57 57 48 41 30 9 3 3
3
BseYI C'CCAG_C
Cuts at: 2148 2364 2910 3008 3186 3656 3901 4086 4569
Size: 216 546 98 178 470 245 185 483
Cuts at: 4569 4791 6728 7224 2148
Size: 222 1937 496 2449
Fragments arranged by size:
2449 1937 546 496 483 470 245 222
216 185 178 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 2909 4046 1163
Size: 573 531 642 1137 4642
Fragments arranged by size:
4642 1137 642 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4975 5736 6764 1540
Size: 275 186 2974 761 1028 2301
Fragments arranged by size:
2974 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2132 5586 5671 6742 2132
Size: 3454 85 1071 2915
Fragments arranged by size:
3454 2915 1071 85
BsmI GAATG_Cn'
Cuts at: 3110 3457 5036 5129 3110
Size: 347 1579 93 5506
Fragments arranged by size:
5506 1579 347 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4663 4663
Size: 7525
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2642 2677 2763 2917 2994 3038
Size: 399 342 510 35 86 154 77 44
Cuts at: 3038 3150 3229 3801 3809 4093 4164 4844 5586
Size: 112 79 572 8 284 71 680 742
Cuts at: 5586 5671 6742 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 680 572 510 399 342
284 154 112 86 85 79 77 71
44 35 8
BspEI T'CCGG_A
Cuts at: 2313 7188 2313
Size: 4875 2650
BspHI T'CATG_A
Cuts at: 5414 7144 5414
Size: 1730 5795
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2412 2481 4018 4336 4880 844
Size: 1568 69 1537 318 544 3489
Fragments arranged by size:
3489 1568 1537 544 318 69
BsrBI CCG'CTC
Cuts at: 1394 1823 2112 3588 4050 5412 1394
Size: 429 289 1476 462 1362 3507
Fragments arranged by size:
3507 1476 1362 462 429 289
BsrDI GCAATG_nn'
Cuts at: 65 1248 2541 2584 2790 6020 6194 65
Size: 1183 1293 43 206 3230 174 1396
Fragments arranged by size:
3230 1396 1293 1183 206 174 43
BsrFI r'CCGG_y
Cuts at: 1700 1810 2429 3195 3205 6169 6356 1700
Size: 110 619 766 10 2964 187 2869
Fragments arranged by size:
2964 2869 766 619 187 110 10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7525
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7525
BssSI C'ACGA_G
Cuts at: 5585 6597 5585
Size: 1012 6513
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7525
BstBI TT'CG_AA
Cuts at: 6329 6329
Size: 7525
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7525
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2167 3791 2011
Size: 156 1624 5745
Fragments arranged by size:
5745 1624 156
BstYI r'GATC_y
Cuts at: 1972 4761 4855 5222 5607 5624 7076 7162 7174
Size: 2789 94 367 385 17 1452 86 12
Cuts at: 7174 7520 1972
Size: 346 1977
Fragments arranged by size:
2789 1977 1452 385 367 346 94 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3166 4306 3166
Size: 1140 6385
BtgI C'CryG_G
Cuts at: 513 1065 2598 3068 3150 3610 3790 4011 4716
Size: 552 1533 470 82 460 180 221 705
Cuts at: 4716 513
Size: 3322
Fragments arranged by size:
3322 1533 705 552 470 460 221 180
82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6786
Fragments arranged by size:
6786 683 56
BtsI GCAGTG_nn'
Cuts at: 2599 4482 5041 5835 5855 2599
Size: 1883 559 794 20 4269
Fragments arranged by size:
4269 1883 794 559 20
ClaI AT'CG_AT
Cuts at: 1554 2931 1554
Size: 1377 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3550 3585 529
Size: 35 2986 35 4469
Fragments arranged by size:
4469 2986 35 35
DraI TTT'AAA
Cuts at: 4829 4829
Size: 7525
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2618 6532 809
Size: 507 1302 3914 1802
Fragments arranged by size:
3914 1802 1302 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2427 2652 3302
Size: 54 1425 50 275 18 597 225 650
Cuts at: 3302 4437 4521 4972 5861 7490 8
Size: 1135 84 451 889 1629 43
Fragments arranged by size:
1629 1425 1135 889 650 597 451 275
225 84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4972 1537
Size: 275 3160 4090
Fragments arranged by size:
4090 3160 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 3217 3264 3541 3556 5455 1353
Size: 867 997 47 277 15 1899 3423
Fragments arranged by size:
3423 1899 997 867 277 47 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6112 6486 6632 387
Size: 1064 138 4523 374 146 1280
Fragments arranged by size:
4523 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2189 2241 2405 2608 2628 3234
Size: 393 422 135 52 164 203 20 606
Cuts at: 3234 3599 4055 4100 4223 4299 4748 5588 6185
Size: 365 456 45 123 76 449 840 597
Cuts at: 6185 6972 7240 1239
Size: 787 268 1524
Fragments arranged by size:
1524 840 787 606 597 456 449 422
393 365 268 203 164 135 123 76
52 45 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2387 3269 2387
Size: 882 6643
EcoO109I rG'GnC_Cy
Cuts at: 2152 2536 2914 3034 3035 4090 4359 2152
Size: 384 378 120 1 1055 269 5318
Fragments arranged by size:
5318 1055 384 378 269 120 1
EcoRI G'AATT_C
Cuts at: 2798 4834 5212 2798
Size: 2036 378 5111
Fragments arranged by size:
5111 2036 378
FseI GG_CCGG'CC
Cuts at: 2433 2433
Size: 7525
FspI TGC'GCA
Cuts at: 6031 6031
Size: 7525
HaeII r_GCGC'y
Cuts at: 1678 2372 3766 3975 6672 1678
Size: 694 1394 209 2697 2531
Fragments arranged by size:
2697 2531 1394 694 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2650 2682 2692 2724 2852
Size: 32 135 32 1078 32 10 32 128
Cuts at: 2852 2884 3149 3181 3308 3338 3340 3370 3655
Size: 32 265 32 127 30 2 30 285
Cuts at: 3655 3687 4697 4729 6166 6198 6240 6272 1373
Size: 32 1010 32 1437 32 42 32 2626
Fragments arranged by size:
2626 1437 1078 1010 285 265 135 128
127 42 32 32 32 32 32 32
32 32 32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4243 4869 5115 669
Size: 988 281 2305 626 246 3079
Fragments arranged by size:
3079 2305 988 626 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7525
HpaI GTT'AAC
Cuts at: 5115 5115
Size: 7525
KasI G'GCGC_C
Cuts at: 2368 3762 2368
Size: 1394 6131
KpnI G_GTAC'C
Cuts at: 4850 4850
Size: 7525
MfeI C'AATT_G
Cuts at: 5124 5124
Size: 7525
MluI A'CGCG_T
Cuts at: 7401 7401
Size: 7525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 2223 2764 3909 4486 4827 6639 6823
Size: 753 165 541 1145 577 341 1812 184
Cuts at: 6823 1305
Size: 2007
Fragments arranged by size:
2007 1812 1145 753 577 541 341 184
165
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2654 10
Size: 54 1425 343 822 4881
Fragments arranged by size:
4881 1425 822 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4586 5483 5842 6001 518
Size: 648 3420 897 359 159 2042
Fragments arranged by size:
3420 2042 897 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2648 2671 3070 3223 3612 3769 4013 4319
Size: 1042 23 399 153 389 157 244 306
Cuts at: 4319 4496 4941 5618 6766 7011 1606
Size: 177 445 677 1148 245 2120
Fragments arranged by size:
2120 1148 1042 677 445 399 389 306
245 244 177 157 153 23
NaeI GCC'GGC
Cuts at: 2431 3207 2431
Size: 776 6749
NarI GG'CG_CC
Cuts at: 2369 3763 2369
Size: 1394 6131
NcoI C'CATG_G
Cuts at: 513 1065 4716 513
Size: 552 3651 3322
Fragments arranged by size:
3651 3322 552
NdeI CA'TA_TG
Cuts at: 387 3307 387
Size: 2920 4605
NgoMIV G'CCGG_C
Cuts at: 2429 3205 2429
Size: 776 6749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7525
NotI GC'GGCC_GC
Cuts at: 4972 4972
Size: 7525
NspI r_CATG'y
Cuts at: 1132 1229 3969 4883 6428 1132
Size: 97 2740 914 1545 2229
Fragments arranged by size:
2740 2229 1545 914 97
PasI CC'CwG_GG
Cuts at: 1348 2635 1348
Size: 1287 6238
PciI A'CATG_T
Cuts at: 6424 6424
Size: 7525
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4105 4765 1108
Size: 2997 660 3868
Fragments arranged by size:
3868 2997 660
PfoI T'CCnGG_A
Cuts at: 2010 3987 4701 4764 2010
Size: 1977 714 63 4771
Fragments arranged by size:
4771 1977 714 63
PmeI GTTT'AAAC
Cuts at: 4829 4829
Size: 7525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3499 5594 5626 7107 7139 3467
Size: 32 2095 32 1481 32 3853
Fragments arranged by size:
3853 2095 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2152 2152
Size: 7525
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7525
PsiI TTA'TAA
Cuts at: 5095 5095
Size: 7525
PspOMI G'GGCC_C
Cuts at: 2673 2913 3034 3225 4089 2673
Size: 240 121 191 864 6109
Fragments arranged by size:
6109 864 240 191 121
PstI C_TGCA'G
Cuts at: 830 3222 4877 830
Size: 2392 1655 3478
Fragments arranged by size:
3478 2392 1655
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7525
PvuII CAG'CTG
Cuts at: 2648 3769 2648
Size: 1121 6404
RsrII CG'GwC_CG
Cuts at: 3691 3691
Size: 7525
SacII CC_GC'GG
Cuts at: 3071 3613 4014 3071
Size: 542 401 6582
Fragments arranged by size:
6582 542 401
SalI G'TCGA_C
Cuts at: 1936 4867 1936
Size: 2931 4594
SanDI GG'GwC_CC
Cuts at: 2152 2152
Size: 7525
SbfI CC_TGCA'GG
Cuts at: 4877 4877
Size: 7525
ScaI AGT'ACT
Cuts at: 2783 5773 7485 2783
Size: 2990 1712 2823
Fragments arranged by size:
2990 2823 1712
SfcI C'TryA_G
Cuts at: 826 1045 3218 4873 6008 6689 6880 826
Size: 219 2173 1655 1135 681 191 1471
Fragments arranged by size:
2173 1655 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2370 3764 2370
Size: 1394 6131
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7525
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7525
SmaI CCC'GGG
Cuts at: 1872 3677 4288 4852 1872
Size: 1805 611 564 4545
Fragments arranged by size:
4545 1805 611 564
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3077 3437 3800 4811 5629 6530
Size: 197 926 1134 360 363 1011 818 901
Cuts at: 6530 6792 820
Size: 262 1553
Fragments arranged by size:
1553 1134 1011 926 901 818 363 360
262 197
SnaBI TAC'GTA
Cuts at: 493 5316 493
Size: 4823 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7525
SphI G_CATG'C
Cuts at: 1132 4883 1132
Size: 3751 3774
SspI AAT'ATT
Cuts at: 5 52 5449 5
Size: 47 5397 2081
Fragments arranged by size:
5397 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7525
StyI C'CwwG_G
Cuts at: 513 1065 2394 2985 3018 3621 4167 4389 4509
Size: 552 1329 591 33 603 546 222 120
Cuts at: 4509 4716 4964 513
Size: 207 248 3074
Fragments arranged by size:
3074 1329 603 591 552 546 248 222
207 120 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3230 3679 4402 4783 5552 5569 5722
Size: 1255 1650 449 723 381 769 17 153
Cuts at: 5722 5907 6352 325
Size: 185 445 1498
Fragments arranged by size:
1650 1498 1255 769 723 449 445 381
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 5771 7483
Size: 274 80 33 51 1443 804 2990 1712
Cuts at: 7483 96
Size: 138
Fragments arranged by size:
2990 1712 1443 804 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 4037 4069 7485 7517 2318
Size: 32 1687 32 3416 32 2326
Fragments arranged by size:
3416 2326 1687 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7525
XbaI T'CTAG_A
Cuts at: 4861 4861
Size: 7525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4516 4723 4766 4516
Size: 207 43 7275
Fragments arranged by size:
7275 207 43
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7525
XmaI C'CCGG_G
Cuts at: 1870 3675 4286 4850 1870
Size: 1805 611 564 4545
Fragments arranged by size:
4545 1805 611 564
XmnI GAAnn'nnTTC
Cuts at: 4781 5654 4781
Size: 873 6652
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5333 276
Size: 53 83 186 936 3799 2468
Fragments arranged by size:
3799 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI
PacI PmlI PspXI PsrI SacI SapI SexAI SfiI
SrfI SwaI