Restriction map for
pFN21AA0417
(Circular) (Six-base) MAPSORT of: pFN21AA0417.seq Check: 2482 from: 1 to: 6730
pFN21AA0417 6730 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3705 4540 278
Size: 53 83 186 936 2169 835 2468
Fragments arranged by size:
2468 2169 936 835 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2187 2967 4073 1937
Size: 250 780 1106 4594
Fragments arranged by size:
4594 1106 780 250
Acc65I G'GTAC_C
Cuts at: 4051 4051
Size: 6730
AclI AA'CG_TT
Cuts at: 1563 4857 5230 1563
Size: 3294 373 3063
Fragments arranged by size:
3294 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2567 4793 6177 6445 1632
Size: 935 2226 1384 268 1917
Fragments arranged by size:
2226 1917 1384 935 268
AflII C'TTAA_G
Cuts at: 820 1017 3008 820
Size: 197 1991 4542
Fragments arranged by size:
4542 1991 197
AflIII A'CryG_T
Cuts at: 1203 5629 6606 1203
Size: 4426 977 1327
Fragments arranged by size:
4426 1327 977
AgeI A'CCGG_T
Cuts at: 5561 5561
Size: 6730
AhdI GACnn_n'nnGTC
Cuts at: 5459 5459
Size: 6730
AleI CACnn'nnGTG
Cuts at: 1166 3908 1166
Size: 2742 3988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3926 1184
Size: 2742 3988
AlwNI CAG_nnn'CTG
Cuts at: 2404 2545 4473 6045 2404
Size: 141 1928 1572 3089
Fragments arranged by size:
3089 1928 1572 141
ApaI G_GGCC'C
Cuts at: 2040 2040
Size: 6730
ApaLI G'TGCA_C
Cuts at: 3694 4787 5943 3694
Size: 1093 1156 4481
Fragments arranged by size:
4481 1156 1093
ApoI r'AATT_y
Cuts at: 1493 4039 4265 4417 6526 1493
Size: 2546 226 152 2109 1697
Fragments arranged by size:
2546 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5284 160
Size: 872 4252 1606
Fragments arranged by size:
4252 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 2324 2684 3413 3851 4055 1870
Size: 73 97 284 360 729 438 204 4545
Fragments arranged by size:
4545 729 438 360 284 204 97 73
BamHI G'GATC_C
Cuts at: 2380 4060 4427 2380
Size: 1680 367 4683
Fragments arranged by size:
4683 1680 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2611 2960 3463 3554 3910
Size: 325 217 627 824 349 503 91 356
Cuts at: 3910 4051 5506 618
Size: 141 1455 1842
Fragments arranged by size:
1842 1455 824 627 503 356 349 325
217 141 91
BanII G_rGCy'C
Cuts at: 1391 2040 4049 1391
Size: 649 2009 4072
Fragments arranged by size:
4072 2009 649
BbeI G_GCGC'C
Cuts at: 3467 3467
Size: 6730
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2303 928
Size: 971 404 5355
Fragments arranged by size:
5355 971 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4921 4955 1903
Size: 34 2984 34 3678
Fragments arranged by size:
3678 2984 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2452 4622 5832 2452
Size: 2170 1210 3350
Fragments arranged by size:
3350 2170 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6730
BfrBI ATG'CAT
Cuts at: 2244 2244
Size: 6730
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2666 5341 136
Size: 107 122 71 631 605 994 2675 1525
Fragments arranged by size:
2675 1525 994 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6725 6725
Size: 6730
BlpI GC'TnA_GC
Cuts at: 2774 4147 2774
Size: 1373 5357
Bme1580I G_kGCm'C
Cuts at: 1790 2040 3261 3698 3915 4791 5947 1790
Size: 250 1221 437 217 876 1156 2573
Fragments arranged by size:
2573 1221 1156 876 437 250 217
BmgBI CAC'GTC
Cuts at: 1318 3532 1318
Size: 2214 4516
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2451 4461 5419
Size: 416 53 83 399 24 1034 2010 958
Cuts at: 5419 442
Size: 1753
Fragments arranged by size:
2010 1753 1034 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6730
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 6730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 2432 2939 5390 1239
Size: 819 374 507 2451 2579
Fragments arranged by size:
2579 2451 819 507 374
Bpu10I CC'TnA_GC
Cuts at: 2841 2841
Size: 6730
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3533 4855 5720 6018 3533
Size: 1322 865 298 4245
Fragments arranged by size:
4245 1322 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5393 882
Size: 617 3894 2219
Fragments arranged by size:
3894 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3640 4521 493
Size: 711 2436 881 2702
Fragments arranged by size:
2702 2436 881 711
BsaBI GATnn'nnATC
Cuts at: 3518 6724 3518
Size: 3206 3524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3464 3702 4537 4919
Size: 53 83 186 936 1931 238 835 382
Cuts at: 4919 5554 275
Size: 635 1451
Fragments arranged by size:
1931 1451 936 835 635 382 238 186
83 53
BsaWI w'CCGG_w
Cuts at: 2988 2995 5163 5561 5835 5982 6393 2988
Size: 7 2168 398 274 147 411 3325
Fragments arranged by size:
3325 2168 411 398 274 147 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 1178
Size: 30 165 30 723 30 5752
Fragments arranged by size:
5752 723 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6730
BseYI C'CCAG_C
Cuts at: 2825 3786 5933 6429 2825
Size: 961 2147 496 3126
Fragments arranged by size:
3126 2147 961 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 3506 1163
Size: 573 681 1089 4387
Fragments arranged by size:
4387 1089 681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3602 4180 4941 5969 1540
Size: 275 186 1601 578 761 1028 2301
Fragments arranged by size:
2301 1601 1028 761 578 275 186
BsiHKAI G_wGCw'C
Cuts at: 3273 3698 3825 4791 4876 5947 3273
Size: 425 127 966 85 1071 4056
Fragments arranged by size:
4056 1071 966 425 127 85
BsmI GAATG_Cn'
Cuts at: 2246 4241 4334 2246
Size: 1995 93 4642
Fragments arranged by size:
4642 1995 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2155 2155
Size: 6730
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 3261 3273 3698 3825 3915 4049
Size: 399 250 1221 12 425 127 90 134
Cuts at: 4049 4791 4876 5947 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1221 1071 742 425 399 250 134
127 90 85 12
BspEI T'CCGG_A
Cuts at: 6393 6393
Size: 6730
BspHI T'CATG_A
Cuts at: 2646 4619 6349 2646
Size: 1973 1730 3027
Fragments arranged by size:
3027 1973 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4085 844
Size: 3241 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3607 4617 1394
Size: 429 1784 1010 3507
Fragments arranged by size:
3507 1784 1010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 3295 5225 5399 65
Size: 1183 1458 589 1930 174 1396
Fragments arranged by size:
1930 1458 1396 1183 589 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2632 3597 5374 5561 1700
Size: 110 211 611 965 1777 187 2869
Fragments arranged by size:
2869 1777 965 611 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6730
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6730
BssSI C'ACGA_G
Cuts at: 2171 4790 5802 2171
Size: 2619 1012 3099
Fragments arranged by size:
3099 2619 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3277 3322 3484 794
Size: 2483 45 162 4040
Fragments arranged by size:
4040 2483 162 45
BstBI TT'CG_AA
Cuts at: 3069 3992 5534 3069
Size: 923 1542 4265
Fragments arranged by size:
4265 1542 923
BstEII G'GTnAC_C
Cuts at: 1184 2273 2991 1184
Size: 1089 718 4923
Fragments arranged by size:
4923 1089 718
BstYI r'GATC_y
Cuts at: 1972 2380 2985 3044 3883 3927 4060 4427 4812
Size: 408 605 59 839 44 133 367 385
Cuts at: 4812 4829 6281 6367 6379 6725 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 839 605 408 385 367 346
133 86 59 44 17 12
Bsu36I CC'TnA_GG
Cuts at: 2721 2721
Size: 6730
BtgI C'CryG_G
Cuts at: 513 1065 2004 2454 3253 3579 513
Size: 552 939 450 799 326 3664
Fragments arranged by size:
3664 939 799 552 450 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2275 487
Size: 683 56 1049 4942
Fragments arranged by size:
4942 1049 683 56
BtsI GCAGTG_nn'
Cuts at: 2405 2815 3903 4246 5040 5060 2405
Size: 410 1088 343 794 20 4075
Fragments arranged by size:
4075 1088 794 410 343 20
ClaI AT'CG_AT
Cuts at: 1554 3372 1554
Size: 1818 4912
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3958 3993 529
Size: 35 3394 35 3266
Fragments arranged by size:
3394 3266 35 35
DraI TTT'AAA
Cuts at: 4034 4034
Size: 6730
DraIII CAC_nnn'GTG
Cuts at: 3265 3690 3265
Size: 425 6305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2147 2975 3581 5737 809
Size: 507 831 828 606 2156 1802
Fragments arranged by size:
2156 1802 831 828 606 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2634 3774
Size: 54 1425 50 275 18 351 453 1140
Cuts at: 3774 4177 5066 6695 8
Size: 403 889 1629 43
Fragments arranged by size:
1629 1425 1140 889 453 403 351 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4177 1537
Size: 275 2365 4090
Fragments arranged by size:
4090 2365 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3989 4660 1353
Size: 2636 671 3423
Fragments arranged by size:
3423 2636 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3048 3866 5317 5691 5837
Size: 1064 138 435 1024 818 1451 374 146
Cuts at: 5837 387
Size: 1280
Fragments arranged by size:
1451 1280 1064 1024 818 435 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2432 2567 2939 4793 5390 6177
Size: 393 426 374 135 372 1854 597 787
Cuts at: 6177 6445 1239
Size: 268 1524
Fragments arranged by size:
1854 1524 787 597 426 393 374 372
268 135
EcoO109I rG'GnC_Cy
Cuts at: 2037 2446 3397 2037
Size: 409 951 5370
Fragments arranged by size:
5370 951 409
EcoRI G'AATT_C
Cuts at: 4039 4417 4039
Size: 378 6352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3454 3454
Size: 6730
FspI TGC'GCA
Cuts at: 5236 5236
Size: 6730
HaeII r_GCGC'y
Cuts at: 1678 2546 3467 5877 1678
Size: 868 921 2410 2531
Fragments arranged by size:
2531 2410 921 868
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 2589 2621 2965
Size: 32 135 32 554 32 431 32 344
Cuts at: 2965 2997 5371 5403 5445 5477 1373
Size: 32 2374 32 42 32 2626
Fragments arranged by size:
2626 2374 554 431 344 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 3145 4074 4320 669
Size: 988 281 250 957 929 246 3079
Fragments arranged by size:
3079 988 957 929 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6730
HpaI GTT'AAC
Cuts at: 4320 4320
Size: 6730
KasI G'GCGC_C
Cuts at: 3463 3463
Size: 6730
KpnI G_GTAC'C
Cuts at: 4055 4055
Size: 6730
MfeI C'AATT_G
Cuts at: 4329 4329
Size: 6730
MluI A'CGCG_T
Cuts at: 6606 6606
Size: 6730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 2327 2852 5844 6028 1305
Size: 864 158 525 2992 184 2007
Fragments arranged by size:
2992 2007 864 525 184 158
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4908
Fragments arranged by size:
4908 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2609 3590 3908 4688 5047 5206
Size: 648 1083 360 981 318 780 359 159
Cuts at: 5206 518
Size: 2042
Fragments arranged by size:
2042 1083 981 780 648 360 359 318
159
MspA1I CmG'CkG
Cuts at: 1606 2805 3205 3255 3581 3790 4146 4823 5971
Size: 1199 400 50 326 209 356 677 1148
Cuts at: 5971 6216 1606
Size: 245 2120
Fragments arranged by size:
2120 1199 1148 677 400 356 326 245
209 50
NaeI GCC'GGC
Cuts at: 2023 2634 2023
Size: 611 6119
NarI GG'CG_CC
Cuts at: 3464 3464
Size: 6730
NcoI C'CATG_G
Cuts at: 513 1065 2004 2454 513
Size: 552 939 450 4789
Fragments arranged by size:
4789 939 552 450
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6730
NgoMIV G'CCGG_C
Cuts at: 2021 2632 2021
Size: 611 6119
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6730
NotI GC'GGCC_GC
Cuts at: 4177 4177
Size: 6730
NsiI A_TGCA'T
Cuts at: 2246 2246
Size: 6730
NspI r_CATG'y
Cuts at: 1132 1229 4088 5633 1132
Size: 97 2859 1545 2229
Fragments arranged by size:
2859 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6730
PciI A'CATG_T
Cuts at: 5629 5629
Size: 6730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2400 3532 3736 1108
Size: 1269 23 1132 204 4102
Fragments arranged by size:
4102 1269 1132 204 23
PfoI T'CCnGG_A
Cuts at: 3393 3393
Size: 6730
PmeI GTTT'AAAC
Cuts at: 4034 4034
Size: 6730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2318 2350 4799 4831 6312 6344 2318
Size: 32 2449 32 1481 32 2704
Fragments arranged by size:
2704 2449 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2446 3397 2446
Size: 951 5779
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6730
PsiI TTA'TAA
Cuts at: 4300 4300
Size: 6730
PspOMI G'GGCC_C
Cuts at: 2036 2036
Size: 6730
PstI C_TGCA'G
Cuts at: 830 2526 3136 3477 4082 830
Size: 1696 610 341 605 3478
Fragments arranged by size:
3478 1696 610 605 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6730
PvuII CAG'CTG
Cuts at: 3790 3790
Size: 6730
SacII CC_GC'GG
Cuts at: 3256 3582 3256
Size: 326 6404
SalI G'TCGA_C
Cuts at: 1936 2186 4072 1936
Size: 250 1886 4594
Fragments arranged by size:
4594 1886 250
SbfI CC_TGCA'GG
Cuts at: 4082 4082
Size: 6730
ScaI AGT'ACT
Cuts at: 2528 4978 6690 2528
Size: 2450 1712 2568
Fragments arranged by size:
2568 2450 1712
SfcI C'TryA_G
Cuts at: 826 1045 2218 2522 3132 3473 3811 4078 5213
Size: 219 1173 304 610 341 338 267 1135
Cuts at: 5213 5894 6085 826
Size: 681 191 1471
Fragments arranged by size:
1471 1173 1135 681 610 341 338 304
267 219 191
SfoI GGC'GCC
Cuts at: 3465 3465
Size: 6730
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6730
SmaI CCC'GGG
Cuts at: 1872 3853 4057 1872
Size: 1981 204 4545
Fragments arranged by size:
4545 1981 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3008 3548 4834 5735 5997 820
Size: 197 926 1065 540 1286 901 262 1553
Fragments arranged by size:
1553 1286 1065 926 901 540 262 197
SnaBI TAC'GTA
Cuts at: 493 4521 493
Size: 4028 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6730
SphI G_CATG'C
Cuts at: 1132 4088 1132
Size: 2956 3774
SspI AAT'ATT
Cuts at: 5 52 3090 4654 5
Size: 47 3038 1564 2081
Fragments arranged by size:
3038 2081 1564 47
StuI AGG'CCT
Cuts at: 1517 2673 1517
Size: 1156 5574
StyI C'CwwG_G
Cuts at: 513 1065 2004 2229 2454 2712 4169 513
Size: 552 939 225 225 258 1457 3074
Fragments arranged by size:
3074 1457 939 552 258 225 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3734 4757 4774 4927 5112 5557 325
Size: 1255 2154 1023 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 1023 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2526 4976 6688
Size: 274 80 33 51 1443 549 2450 1712
Cuts at: 6688 96
Size: 138
Fragments arranged by size:
2450 1712 1443 549 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6690 6722 6690
Size: 32 6698
Tth111I GACn'n_nGTC
Cuts at: 1316 3830 1316
Size: 2514 4216
XbaI T'CTAG_A
Cuts at: 4066 4066
Size: 6730
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6730
XmaI C'CCGG_G
Cuts at: 1870 3851 4055 1870
Size: 1981 204 4545
Fragments arranged by size:
4545 1981 204
XmnI GAAnn'nnTTC
Cuts at: 3075 4859 3075
Size: 1784 4946
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3703 4538 276
Size: 53 83 186 936 2169 835 2468
Fragments arranged by size:
2468 2169 936 835 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI
BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NruI
PacI PmlI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SrfI SwaI XcmI