Restriction map for
pFN21AA0412
(Circular) (Six-base) MAPSORT of: pFN21AA0412.seq Check: 9736 from: 1 to: 6586
pFN21AA0412 6586 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4396 278
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3929 1937
Size: 1992 4594
Acc65I G'GTAC_C
Cuts at: 3907 3907
Size: 6586
AclI AA'CG_TT
Cuts at: 1563 4713 5086 1563
Size: 3150 373 3063
Fragments arranged by size:
3150 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2315 2407 3409 3631 4649 6033 6301 1632
Size: 683 92 1002 222 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1018 1002 683 268 222 92
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6389
AflIII A'CryG_T
Cuts at: 1203 5485 6462 1203
Size: 4282 977 1327
Fragments arranged by size:
4282 1327 977
AgeI A'CCGG_T
Cuts at: 5417 5417
Size: 6586
AhdI GACnn_n'nnGTC
Cuts at: 5315 5315
Size: 6586
AleI CACnn'nnGTG
Cuts at: 1166 3018 1166
Size: 1852 4734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6586
AlwNI CAG_nnn'CTG
Cuts at: 4329 5901 4329
Size: 1572 5014
ApaI G_GGCC'C
Cuts at: 2035 3349 2035
Size: 1314 5272
ApaLI G'TGCA_C
Cuts at: 2953 4643 5799 2953
Size: 1690 1156 3740
Fragments arranged by size:
3740 1690 1156
ApoI r'AATT_y
Cuts at: 1493 2673 3895 4121 4273 6382 1493
Size: 1180 1222 226 152 2109 1697
Fragments arranged by size:
2109 1697 1222 1180 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5140 160
Size: 872 4108 1606
Fragments arranged by size:
4108 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
AvaI C'yCGr_G
Cuts at: 1870 1943 3209 3911 1870
Size: 73 1266 702 4545
Fragments arranged by size:
4545 1266 702 73
BamHI G'GATC_C
Cuts at: 3916 4283 3916
Size: 367 6219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3084 3252 3907 5362 618
Size: 325 217 627 1297 168 655 1455 1842
Fragments arranged by size:
1842 1455 1297 655 627 325 217 168
BanII G_rGCy'C
Cuts at: 1391 2035 2151 2471 3349 3905 1391
Size: 644 116 320 878 556 4072
Fragments arranged by size:
4072 878 644 556 320 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2241 3049 3133 3730 3870 928
Size: 971 342 808 84 597 140 3644
Fragments arranged by size:
3644 971 808 597 342 140 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4777 4811 1903
Size: 34 2840 34 3678
Fragments arranged by size:
3678 2840 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4478 5688 4478
Size: 1210 5376
BclI T'GATC_A
Cuts at: 1548 3844 1548
Size: 2296 4290
BfrBI ATG'CAT
Cuts at: 3685 3685
Size: 6586
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5197 136
Size: 107 122 71 631 605 337 3188 1525
Fragments arranged by size:
3188 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6581 6581
Size: 6586
BlpI GC'TnA_GC
Cuts at: 2106 2184 4003 2106
Size: 78 1819 4689
Fragments arranged by size:
4689 1819 78
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2957 3349 4647 5803 1790
Size: 245 922 392 1298 1156 2573
Fragments arranged by size:
2573 1298 1156 922 392 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6586
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2775 3279 3699
Size: 416 53 83 399 24 1358 504 420
Cuts at: 3699 4317 5275 442
Size: 618 958 1753
Fragments arranged by size:
1753 1358 958 618 504 420 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2548 2341
Size: 207 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2520 2703 2955 3207 3375 3459 3589 3627
Size: 1281 183 252 252 168 84 130 38
Cuts at: 3627 5246 1239
Size: 1619 2579
Fragments arranged by size:
2579 1619 1281 252 252 183 168 130
84 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3230 4711 5576 5874 3230
Size: 1481 865 298 3942
Fragments arranged by size:
3942 1481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5249 882
Size: 617 3750 2219
Fragments arranged by size:
3750 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4377 493
Size: 711 3173 2702
Fragments arranged by size:
3173 2702 711
BsaBI GATnn'nnATC
Cuts at: 6580 6580
Size: 6586
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4393 4775 5410 275
Size: 53 83 186 936 2860 382 635 1451
Fragments arranged by size:
2860 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5019 5417 5691 5838 6249 5019
Size: 398 274 147 411 5356
Fragments arranged by size:
5356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2532 2562 1178
Size: 30 165 30 1129 30 5202
Fragments arranged by size:
5202 1129 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 1186
Size: 910 5676
BseYI C'CCAG_C
Cuts at: 2829 5789 6285 2829
Size: 2960 496 3130
Fragments arranged by size:
3130 2960 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3142 3174 3310 1163
Size: 573 1406 32 136 4439
Fragments arranged by size:
4439 1406 573 136 32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4036 4797 5825 1540
Size: 275 186 2035 761 1028 2301
Fragments arranged by size:
2301 2035 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2735 2957 3575 4647 4732 5803 2735
Size: 222 618 1072 85 1071 3518
Fragments arranged by size:
3518 1072 1071 618 222 85
BsmI GAATG_Cn'
Cuts at: 2705 2882 4097 4190 2705
Size: 177 1215 93 5101
Fragments arranged by size:
5101 1215 177 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2151 2471 2735 2957 3349 3575
Size: 399 245 116 320 264 222 392 226
Cuts at: 3575 3905 4647 4732 5803 1391
Size: 330 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 392 330 320 264
245 226 222 116 85
BspEI T'CCGG_A
Cuts at: 6249 6249
Size: 6586
BspHI T'CATG_A
Cuts at: 4475 6205 4475
Size: 1730 4856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3941 844
Size: 3097 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2901 4473 1394
Size: 429 1078 1572 3507
Fragments arranged by size:
3507 1572 1078 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2878 5081 5255 65
Size: 1183 1630 2203 174 1396
Fragments arranged by size:
2203 1630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5230 5417 1700
Size: 110 3420 187 2869
Fragments arranged by size:
3420 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6586
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6586
BssSI C'ACGA_G
Cuts at: 3499 4646 5658 3499
Size: 1147 1012 4427
Fragments arranged by size:
4427 1147 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2877 794
Size: 2083 4503
BstBI TT'CG_AA
Cuts at: 5390 5390
Size: 6586
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6586
BstYI r'GATC_y
Cuts at: 1972 2437 2581 3916 4283 4668 4685 6137 6223
Size: 465 144 1335 367 385 17 1452 86
Cuts at: 6223 6235 6581 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1335 465 385 367 346 144
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2219 513
Size: 552 939 215 4880
Fragments arranged by size:
4880 939 552 215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5847
Fragments arranged by size:
5847 683 56
BtsI GCAGTG_nn'
Cuts at: 2626 3130 3298 3316 4102 4896 4916 2626
Size: 504 168 18 786 794 20 4296
Fragments arranged by size:
4296 794 786 504 168 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6586
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3364 3399 529
Size: 35 2800 35 3716
Fragments arranged by size:
3716 2800 35 35
DraI TTT'AAA
Cuts at: 2614 3890 2614
Size: 1276 5310
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5593 809
Size: 507 4277 1802
Fragments arranged by size:
4277 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2965 4033 4922
Size: 54 1425 50 275 18 1135 1068 889
Cuts at: 4922 6551 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1135 1068 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4033 1537
Size: 275 2221 4090
Fragments arranged by size:
4090 2221 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2592 4516 1353
Size: 1239 1924 3423
Fragments arranged by size:
3423 1924 1239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5173 5547 5693 387
Size: 1064 138 3584 374 146 1280
Fragments arranged by size:
3584 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2407 2520 2703 2955 3207 3375
Size: 393 683 92 113 183 252 252 168
Cuts at: 3375 3409 3459 3589 3627 3631 4649 5246 6033
Size: 34 50 130 38 4 1018 597 787
Cuts at: 6033 6301 1239
Size: 268 1524
Fragments arranged by size:
1524 1018 787 683 597 393 268 252
252 183 168 130 113 92 50 38
34 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 6586
EcoO109I rG'GnC_Cy
Cuts at: 2775 3345 3409 2775
Size: 570 64 5952
Fragments arranged by size:
5952 570 64
EcoRI G'AATT_C
Cuts at: 3895 4273 3895
Size: 378 6208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 6586
FspI TGC'GCA
Cuts at: 5092 5092
Size: 6586
HaeII r_GCGC'y
Cuts at: 1678 5733 1678
Size: 4055 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 2532 2564 2722
Size: 32 135 32 812 32 116 32 158
Cuts at: 2722 2754 3228 3260 3812 3844 5227 5259 5301
Size: 32 474 32 552 32 1383 32 42
Cuts at: 5301 5333 1373
Size: 32 2626
Fragments arranged by size:
2626 1383 812 552 474 158 135 116
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3930 4176 669
Size: 988 281 1992 246 3079
Fragments arranged by size:
3079 1992 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6586
HpaI GTT'AAC
Cuts at: 4176 4176
Size: 6586
KpnI G_GTAC'C
Cuts at: 3911 3911
Size: 6586
MfeI C'AATT_G
Cuts at: 4185 4185
Size: 6586
MluI A'CGCG_T
Cuts at: 6462 6462
Size: 6586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5700 5884 1305
Size: 4395 184 2007
Fragments arranged by size:
4395 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2967 10
Size: 54 1425 343 1135 3629
Fragments arranged by size:
3629 1425 1135 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3018 3171 3485 4544 4903 5062 518
Size: 648 1852 153 314 1059 359 159 2042
Fragments arranged by size:
2042 1852 1059 648 359 314 159 153
MspA1I CmG'CkG
Cuts at: 1606 2015 2095 2110 2495 2839 3259 4002 4679
Size: 409 80 15 385 344 420 743 677
Cuts at: 4679 5827 6072 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 743 677 420 409 385 344
245 80 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 2219 513
Size: 552 939 215 4880
Fragments arranged by size:
4880 939 552 215
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6586
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6586
NotI GC'GGCC_GC
Cuts at: 4033 4033
Size: 6586
NsiI A_TGCA'T
Cuts at: 3687 3687
Size: 6586
NspI r_CATG'y
Cuts at: 1132 1229 3490 3593 3944 5489 1132
Size: 97 2261 103 351 1545 2229
Fragments arranged by size:
2261 2229 1545 351 103 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6586
PciI A'CATG_T
Cuts at: 5485 5485
Size: 6586
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6586
PfoI T'CCnGG_A
Cuts at: 2536 2536
Size: 6586
PmeI GTTT'AAAC
Cuts at: 3890 3890
Size: 6586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4655 4687 6168 6200 4655
Size: 32 1481 32 5041
Fragments arranged by size:
5041 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3409 3409
Size: 6586
PshAI GACnn'nnGTC
Cuts at: 1613 2253 1613
Size: 640 5946
PsiI TTA'TAA
Cuts at: 4156 4156
Size: 6586
PspOMI G'GGCC_C
Cuts at: 2031 3345 2031
Size: 1314 5272
PspXI vC'TCGA_Gb
Cuts at: 3209 3209
Size: 6586
PstI C_TGCA'G
Cuts at: 830 3938 830
Size: 3108 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6586
PvuII CAG'CTG
Cuts at: 2095 2495 2095
Size: 400 6186
RsrII CG'GwC_CG
Cuts at: 2026 2026
Size: 6586
SalI G'TCGA_C
Cuts at: 1936 3928 1936
Size: 1992 4594
SbfI CC_TGCA'GG
Cuts at: 3938 3938
Size: 6586
ScaI AGT'ACT
Cuts at: 4834 6546 4834
Size: 1712 4874
SfcI C'TryA_G
Cuts at: 826 1045 3934 5069 5750 5941 826
Size: 219 2889 1135 681 191 1471
Fragments arranged by size:
2889 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6586
SmaI CCC'GGG
Cuts at: 1872 3913 1872
Size: 2041 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3209 3245 4690 5591 5853 820
Size: 197 926 1266 36 1445 901 262 1553
Fragments arranged by size:
1553 1445 1266 926 901 262 197 36
SnaBI TAC'GTA
Cuts at: 493 4377 493
Size: 3884 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6586
SphI G_CATG'C
Cuts at: 1132 3944 1132
Size: 2812 3774
SspI AAT'ATT
Cuts at: 5 52 3811 4510 5
Size: 47 3759 699 2081
Fragments arranged by size:
3759 2081 699 47
StuI AGG'CCT
Cuts at: 1517 2804 2888 3308 3476 3644 1517
Size: 1287 84 420 168 168 4459
Fragments arranged by size:
4459 1287 420 168 168 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2076 2219 4025 513
Size: 552 939 72 143 1806 3074
Fragments arranged by size:
3074 1806 939 552 143 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2909 3329 4613 4630 4783 4968 5413
Size: 1255 1329 420 1284 17 153 185 445
Cuts at: 5413 325
Size: 1498
Fragments arranged by size:
1498 1329 1284 1255 445 420 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4832 6544 96
Size: 274 80 33 51 1443 2855 1712 138
Fragments arranged by size:
2855 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2375 2833 2865 3505 3537 6546 6578 2343
Size: 32 458 32 640 32 3009 32 2351
Fragments arranged by size:
3009 2351 640 458 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6586
XbaI T'CTAG_A
Cuts at: 3922 3922
Size: 6586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2204 2204
Size: 6586
XhoI C'TCGA_G
Cuts at: 1943 3209 1943
Size: 1266 5320
XmaI C'CCGG_G
Cuts at: 1870 3911 1870
Size: 2041 4545
XmnI GAAnn'nnTTC
Cuts at: 3140 3224 3281 4715 3140
Size: 84 57 1434 5011
Fragments arranged by size:
5011 1434 84 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4394 276
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PsrI SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI