Restriction map for
pFN21AA0407
(Circular) (Six-base) MAPSORT of: pFN21AA0407.seq Check: 7490 from: 1 to: 11110
pFN21AA0407 11110 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3513 4952 6031 3513
Size: 1439 1079 8592
Fragments arranged by size:
8592 1439 1079
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8920 278
Size: 53 83 186 936 7384 2468
Fragments arranged by size:
7384 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3297 5250 5747 7091 8453 1937
Size: 1360 1953 497 1344 1362 4594
Fragments arranged by size:
4594 1953 1362 1360 1344 497
Acc65I G'GTAC_C
Cuts at: 8431 8431
Size: 11110
AclI AA'CG_TT
Cuts at: 1563 9237 9610 1563
Size: 7674 373 3063
Fragments arranged by size:
7674 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3449 4321 9173 10557 10825 1632
Size: 1817 872 4852 1384 268 1917
Fragments arranged by size:
4852 1917 1817 1384 872 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10913
AflIII A'CryG_T
Cuts at: 1203 10009 10986 1203
Size: 8806 977 1327
Fragments arranged by size:
8806 1327 977
AgeI A'CCGG_T
Cuts at: 9941 9941
Size: 11110
AhdI GACnn_n'nnGTC
Cuts at: 5456 8180 9839 5456
Size: 2724 1659 6727
Fragments arranged by size:
6727 2724 1659
AleI CACnn'nnGTG
Cuts at: 1166 3688 5429 1166
Size: 2522 1741 6847
Fragments arranged by size:
6847 2522 1741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2123 3372 4756 4838 1184
Size: 939 1249 1384 82 7456
Fragments arranged by size:
7456 1384 1249 939 82
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7796 7828 7796
Size: 32 11078
AlwNI CAG_nnn'CTG
Cuts at: 2116 2238 2395 3460 3948 3979 4209 4229 4418
Size: 122 157 1065 488 31 230 20 189
Cuts at: 4418 5097 5833 6337 6559 7046 7489 8384 8853
Size: 679 736 504 222 487 443 895 469
Cuts at: 8853 10425 2116
Size: 1572 2801
Fragments arranged by size:
2801 1572 1065 895 736 679 504 488
487 469 443 230 222 189 157 122
31 20
ApaI G_GGCC'C
Cuts at: 2021 2326 2569 3308 3563 4014 4646 2021
Size: 305 243 739 255 451 632 8485
Fragments arranged by size:
8485 739 632 451 305 255 243
ApaLI G'TGCA_C
Cuts at: 2345 3096 3982 9167 10323 2345
Size: 751 886 5185 1156 3132
Fragments arranged by size:
5185 3132 1156 886 751
ApoI r'AATT_y
Cuts at: 1493 6065 6632 8419 8645 8797 10906 1493
Size: 4572 567 1787 226 152 2109 1697
Fragments arranged by size:
4572 2109 1787 1697 567 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9664 160
Size: 872 8632 1606
Fragments arranged by size:
8632 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11110
AvaI C'yCGr_G
Cuts at: 1870 1943 2384 2562 2751 3030 3547 4424 4553
Size: 73 441 178 189 279 517 877 129
Cuts at: 4553 4697 4880 4899 5163 5875 6897 8249 8435
Size: 144 183 19 264 712 1022 1352 186
Cuts at: 8435 1870
Size: 4545
Fragments arranged by size:
4545 1352 1022 877 712 517 441 279
264 189 186 183 178 144 129 73
19
BamHI G'GATC_C
Cuts at: 5485 8440 8807 5485
Size: 2955 367 7788
Fragments arranged by size:
7788 2955 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2370 5258 5420 5437
Size: 325 217 627 351 232 2888 162 17
Cuts at: 5437 5534 6300 6345 6364 6491 6741 7443 7497
Size: 97 766 45 19 127 250 702 54
Cuts at: 7497 7656 7784 8431 9886 618
Size: 159 128 647 1455 1842
Fragments arranged by size:
2888 1842 1455 766 702 647 627 351
325 250 232 217 162 159 128 127
97 54 45 19 17
BanII G_rGCy'C
Cuts at: 1391 2021 2183 2318 2326 2496 2569 3308 3563
Size: 630 162 135 8 170 73 739 255
Cuts at: 3563 3840 4014 4037 4150 4281 4505 4646 4704
Size: 277 174 23 113 131 224 141 58
Cuts at: 4704 4769 4989 5282 6321 6449 8429 1391
Size: 65 220 293 1039 128 1980 4072
Fragments arranged by size:
4072 1980 1039 739 630 293 277 255
224 220 174 170 162 141 135 131
128 113 73 65 58 23 8
BbeI G_GCGC'C
Cuts at: 2374 5424 7501 2374
Size: 3050 2077 5983
Fragments arranged by size:
5983 3050 2077
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6293 7940 928
Size: 971 4394 1647 4098
Fragments arranged by size:
4394 4098 1647 971
BbvCI CC'TCA_GC
Cuts at: 3045 4345 5040 5184 3045
Size: 1300 695 144 8971
Fragments arranged by size:
8971 1300 695 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2284 2318 3003 3037 5866
Size: 34 50 34 263 34 685 34 2829
Cuts at: 5866 5900 9301 9335 1903
Size: 34 3401 34 3678
Fragments arranged by size:
3678 3401 2829 685 263 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2510 3832 9002 10212 2510
Size: 1322 5170 1210 3408
Fragments arranged by size:
5170 3408 1322 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11110
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2410 2621 6181
Size: 107 122 71 631 605 738 211 3560
Cuts at: 6181 6914 7693 7716 9721 136
Size: 733 779 23 2005 1525
Fragments arranged by size:
3560 2005 1525 779 738 733 631 605
211 122 107 71 23
BglII A'GATC_T
Cuts at: 7752 8404 11105 7752
Size: 652 2701 7757
Fragments arranged by size:
7757 2701 652
BlpI GC'TnA_GC
Cuts at: 2177 3453 4202 4961 8527 2177
Size: 1276 749 759 3566 4760
Fragments arranged by size:
4760 3566 1276 759 749
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2270 2326 2349 2569 3100 3308 3563
Size: 231 249 56 23 220 531 208 255
Cuts at: 3563 3770 3986 4014 4407 4449 4646 5207 5340
Size: 207 216 28 393 42 197 561 133
Cuts at: 5340 5537 6494 6744 7308 7446 7787 9171 10327
Size: 197 957 250 564 138 341 1384 1156
Cuts at: 10327 1790
Size: 2573
Fragments arranged by size:
2573 1384 1156 957 564 561 531 393
341 255 250 249 231 220 216 208
207 197 197 138 133 56 42 28
23
BmgBI CAC'GTC
Cuts at: 1318 2853 5453 5807 5839 7999 1318
Size: 1535 2600 354 32 2160 4429
Fragments arranged by size:
4429 2600 2160 1535 354 32
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3788 4924 5110
Size: 416 53 83 399 24 2371 1136 186
Cuts at: 5110 5194 5250 5580 6994 8841 9799 442
Size: 84 56 330 1414 1847 958 1753
Fragments arranged by size:
2371 1847 1753 1414 1136 958 416 399
330 186 84 83 56 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 4297 7400 8224 2777
Size: 1520 3103 824 5663
Fragments arranged by size:
5663 3103 1520 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2015 2048 2216 2973 3218 4892 4905 5352
Size: 776 33 168 757 245 1674 13 447
Cuts at: 5352 5356 5600 6611 6620 6893 7623 7643 8342
Size: 4 244 1011 9 273 730 20 699
Cuts at: 8342 8351 9770 1239
Size: 9 1419 2579
Fragments arranged by size:
2579 1674 1419 1011 776 757 730 699
447 273 245 244 168 33 20 13
9 9 4
Bpu10I CC'TnA_GC
Cuts at: 3045 3591 4345 5040 5184 3045
Size: 546 754 695 144 8971
Fragments arranged by size:
8971 754 695 546 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4658 6971 9235 10100 10398 4658
Size: 2313 2264 865 298 5370
Fragments arranged by size:
5370 2313 2264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3363 5374 5598 5651 5878 8238 9773
Size: 617 1864 2011 224 53 227 2360 1535
Cuts at: 9773 882
Size: 2219
Fragments arranged by size:
2360 2219 2011 1864 1535 617 227 224
53
BsaAI yAC'GTr
Cuts at: 493 1204 2103 2675 3820 6012 6427 6913 8901
Size: 711 899 572 1145 2192 415 486 1988
Cuts at: 8901 493
Size: 2702
Fragments arranged by size:
2702 2192 1988 1145 899 711 572 486
415
BsaBI GATnn'nnATC
Cuts at: 3126 7079 11104 3126
Size: 3953 4025 3132
Fragments arranged by size:
4025 3953 3132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2371 5421 5849 5914
Size: 53 83 186 936 838 3050 428 65
Cuts at: 5914 7498 8058 8917 9299 9934 275
Size: 1584 560 859 382 635 1451
Fragments arranged by size:
3050 1584 1451 936 859 838 635 560
428 382 186 83 65 53
BsaWI w'CCGG_w
Cuts at: 8240 9543 9941 10215 10362 10773 8240
Size: 1303 398 274 147 411 8577
Fragments arranged by size:
8577 1303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 3314 3344 4102
Size: 30 165 30 1362 30 519 30 758
Cuts at: 4102 4132 5354 5384 5621 5651 6012 6042 7109
Size: 30 1222 30 237 30 361 30 1067
Cuts at: 7109 7139 7319 7349 7527 7557 1178
Size: 30 180 30 178 30 4731
Fragments arranged by size:
4731 1362 1222 1067 758 519 361 237
180 178 165 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 2887 3659 3822 4289 4598 5423 5986
Size: 1435 266 772 163 467 309 825 563
Cuts at: 5986 5990 6260 8339 1186
Size: 4 270 2079 3957
Fragments arranged by size:
3957 2079 1435 825 772 563 467 309
270 266 163 4
BseYI C'CCAG_C
Cuts at: 2020 2277 2942 3152 3595 3948 4007 4073 4289
Size: 257 665 210 443 353 59 66 216
Cuts at: 4289 5206 5311 5528 5891 6000 6811 7389 7890
Size: 917 105 217 363 109 811 578 501
Cuts at: 7890 8083 8185 8353 10313 10809 2020
Size: 193 102 168 1960 496 2321
Fragments arranged by size:
2321 1960 917 811 665 578 501 496
443 363 353 257 217 216 210 193
168 109 105 102 66 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2889 3272 3547 4808 5321 6413 6449
Size: 573 1153 383 275 1261 513 1092 36
Cuts at: 6449 8047 1163
Size: 1598 4226
Fragments arranged by size:
4226 1598 1261 1153 1092 573 513 383
275 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5020 8560 9321 10349 1540
Size: 275 186 3019 3540 761 1028 2301
Fragments arranged by size:
3540 3019 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2349 2883 3100 3399 3422 3454 3516 3548 3840
Size: 534 217 299 23 32 62 32 292
Cuts at: 3840 3986 4989 7578 8031 9171 9256 10327 2349
Size: 146 1003 2589 453 1140 85 1071 3132
Fragments arranged by size:
3132 2589 1140 1071 1003 534 453 299
292 217 146 85 62 32 32 23
BsmI GAATG_Cn'
Cuts at: 2084 2544 5886 7264 8621 8714 2084
Size: 460 3342 1378 1357 93 4480
Fragments arranged by size:
4480 3342 1378 1357 460 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3641 5546 5924 3641
Size: 1905 378 8827
Fragments arranged by size:
8827 1905 378
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2183 2270 2318 2326 2349 2496
Size: 399 231 162 87 48 8 23 147
Cuts at: 2496 2569 2883 3100 3308 3399 3422 3454 3516
Size: 73 314 217 208 91 23 32 62
Cuts at: 3516 3548 3563 3770 3840 3986 4014 4037 4150
Size: 32 15 207 70 146 28 23 113
Cuts at: 4150 4281 4407 4449 4505 4646 4704 4769 4989
Size: 131 126 42 56 141 58 65 220
Cuts at: 4989 5207 5282 5340 5537 6321 6449 6494 6744
Size: 218 75 58 197 784 128 45 250
Cuts at: 6744 7308 7446 7578 7787 8031 8429 9171 9256
Size: 564 138 132 209 244 398 742 85
Cuts at: 9256 10327 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 784 742 564 399 398 314
250 244 231 220 218 217 209 208
207 197 162 147 146 141 138 132
131 128 126 113 91 87 85 75
73 70 65 62 58 58 56 48
45 42 32 32 28 23 23 23
15 8
BspEI T'CCGG_A
Cuts at: 8240 10773 8240
Size: 2533 8577
BspHI T'CATG_A
Cuts at: 3408 6645 8999 10729 3408
Size: 3237 2354 1730 3789
Fragments arranged by size:
3789 3237 2354 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2721 3513 3687 4214 4464 4830 4952 6031
Size: 1877 792 174 527 250 366 122 1079
Cuts at: 6031 6837 7881 8465 844
Size: 806 1044 584 3489
Fragments arranged by size:
3489 1877 1079 1044 806 792 584 527
366 250 174 122
BsrBI CCG'CTC
Cuts at: 1394 1823 2410 4795 5719 7609 7849 8997 1394
Size: 429 587 2385 924 1890 240 1148 3507
Fragments arranged by size:
3507 2385 1890 1148 924 587 429 240
BsrDI GCAATG_nn'
Cuts at: 65 1248 6240 8037 9605 9779 65
Size: 1183 4992 1797 1568 174 1396
Fragments arranged by size:
4992 1797 1568 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2213 2922 2994 4780 5015 5417 9754
Size: 110 403 709 72 1786 235 402 4337
Cuts at: 9754 9941 1700
Size: 187 2869
Fragments arranged by size:
4337 2869 1786 709 403 402 235 187
110 72
BsrGI T'GTAC_A
Cuts at: 96 6534 7125 96
Size: 6438 591 4081
Fragments arranged by size:
6438 4081 591
BssHII G'CGCG_C
Cuts at: 1925 7732 1925
Size: 5807 5303
BssSI C'ACGA_G
Cuts at: 3997 4531 5381 9170 10182 3997
Size: 534 850 3789 1012 4925
Fragments arranged by size:
4925 3789 1012 850 534
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2238 2395 5097 5308 6337 794
Size: 1444 157 2702 211 1029 5567
Fragments arranged by size:
5567 2702 1444 1029 211 157
BstBI TT'CG_AA
Cuts at: 9914 9914
Size: 11110
BstEII G'GTnAC_C
Cuts at: 1184 5863 1184
Size: 4679 6431
BstXI CCAn_nnnn'nTGG
Cuts at: 2863 3160 3570 3690 4105 5431 5903 7898 2863
Size: 297 410 120 415 1326 472 1995 6075
Fragments arranged by size:
6075 1995 1326 472 415 410 297 120
BstYI r'GATC_y
Cuts at: 1972 5485 6582 6726 7752 8404 8440 8807 9192
Size: 3513 1097 144 1026 652 36 367 385
Cuts at: 9192 9209 10661 10747 10759 11105 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3513 1977 1452 1097 1026 652 385 367
346 144 86 36 17 12
BstZ17I GTA'TAC
Cuts at: 5748 7092 5748
Size: 1344 9766
Bsu36I CC'TnA_GG
Cuts at: 2134 4356 6042 6159 6560 2134
Size: 2222 1686 117 401 6684
Fragments arranged by size:
6684 2222 1686 401 117
BtgI C'CryG_G
Cuts at: 513 1065 3159 3237 4014 4134 4217 4497 5073
Size: 552 2094 78 777 120 83 280 576
Cuts at: 5073 5515 5712 5821 5868 6207 6324 6341 6930
Size: 442 197 109 47 339 117 17 589
Cuts at: 6930 513
Size: 4693
Fragments arranged by size:
4693 2094 777 589 576 552 442 339
280 197 120 117 109 83 78 47
17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3241 3330 487
Size: 683 56 2015 89 8267
Fragments arranged by size:
8267 2015 683 89 56
BtsI GCAGTG_nn'
Cuts at: 2631 3366 3575 3929 4407 6613 6935 7508 8313
Size: 735 209 354 478 2206 322 573 805
Cuts at: 8313 8626 9420 9440 2631
Size: 313 794 20 4301
Fragments arranged by size:
4301 2206 805 794 735 573 478 354
322 313 209 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11075
DraI TTT'AAA
Cuts at: 8414 8414
Size: 11110
DraIII CAC_nnn'GTG
Cuts at: 2659 5156 5202 5227 7156 7663 2659
Size: 2497 46 25 1929 507 6106
Fragments arranged by size:
6106 2497 1929 507 46 25
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5913 6053 6083 7466 10117 809
Size: 507 4597 140 30 1383 2651 1802
Fragments arranged by size:
4597 2651 1802 1383 507 140 30
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2402 2573 2815
Size: 54 1425 50 275 18 572 171 242
Cuts at: 2815 2847 3162 3203 3319 3692 4107 5017 5193
Size: 32 315 41 116 373 415 910 176
Cuts at: 5193 5415 5824 7008 7394 7437 8070 8557 9446
Size: 222 409 1184 386 43 633 487 889
Cuts at: 9446 11075 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1184 910 889 633 572 487
415 409 386 373 315 275 242 222
176 171 116 54 50 43 43 41
32 18
EagI C'GGCC_G
Cuts at: 1537 1812 5017 8557 1537
Size: 275 3205 3540 4090
Fragments arranged by size:
4090 3540 3205 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 4437 4710 5440 6504 6539 6851 9040
Size: 678 2406 273 730 1064 35 312 2189
Cuts at: 9040 1353
Size: 3423
Fragments arranged by size:
3423 2406 2189 1064 730 678 312 273
35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3899 4574 9697 10071 10217 387
Size: 1064 138 2310 675 5123 374 146 1280
Fragments arranged by size:
5123 2310 1280 1064 675 374 146 138
EcoICRI GAG'CTC
Cuts at: 3838 4987 3838
Size: 1149 9961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2048 2216 2973 3218 3449 4321
Size: 393 383 33 168 757 245 231 872
Cuts at: 4321 4892 4905 5352 5356 5600 6611 6620 6893
Size: 571 13 447 4 244 1011 9 273
Cuts at: 6893 7623 7643 8342 8351 9173 9770 10557 10825
Size: 730 20 699 9 822 597 787 268
Cuts at: 10825 1239
Size: 1524
Fragments arranged by size:
1524 1011 872 822 787 757 730 699
597 571 447 393 383 273 268 245
244 231 168 33 20 13 9 9
4
EcoNI CCTnn'n_nnAGG
Cuts at: 2984 4062 4832 6041 6359 7099 7284 7814 8324
Size: 1078 770 1209 318 740 185 530 510
Cuts at: 8324 2984
Size: 5770
Fragments arranged by size:
5770 1209 1078 770 740 530 510 318
185
EcoO109I rG'GnC_Cy
Cuts at: 2053 2125 2322 2323 2566 2710 3172 3387 3490
Size: 72 197 1 243 144 462 215 103
Cuts at: 3490 3559 3790 4360 4642 4643 4876 6046 6163
Size: 69 231 570 282 1 233 1170 117
Cuts at: 6163 6556 6766 7277 7315 7525 2053
Size: 393 210 511 38 210 5638
Fragments arranged by size:
5638 1170 570 511 462 393 282 243
233 231 215 210 210 197 144 117
103 72 69 38 1 1
EcoRI G'AATT_C
Cuts at: 6632 8419 8797 6632
Size: 1787 378 8945
Fragments arranged by size:
8945 1787 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5767 7882 5767
Size: 2115 8995
FseI GG_CCGG'CC
Cuts at: 2926 5019 2926
Size: 2093 9017
FspI TGC'GCA
Cuts at: 3921 7034 9616 3921
Size: 3113 2582 5415
Fragments arranged by size:
5415 3113 2582
HaeII r_GCGC'y
Cuts at: 1678 2374 2688 3852 5213 5424 7501 10257 1678
Size: 696 314 1164 1361 211 2077 2756 2531
Fragments arranged by size:
2756 2531 2077 1361 1164 696 314 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 3240 3272 3455
Size: 32 135 32 545 32 1091 32 183
Cuts at: 3455 3487 3806 3838 4610 4642 5442 5474 5621
Size: 32 319 32 772 32 800 32 147
Cuts at: 5621 5653 6646 6678 7319 7351 7412 7444 8166
Size: 32 993 32 641 32 61 32 722
Cuts at: 8166 8198 9751 9783 9825 9857 1373
Size: 32 1553 32 42 32 2626
Fragments arranged by size:
2626 1553 1091 993 800 772 722 641
545 319 183 147 135 61 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5992 8454 8700 669
Size: 988 281 4054 2462 246 3079
Fragments arranged by size:
4054 3079 2462 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6947 748
Size: 6199 4911
HpaI GTT'AAC
Cuts at: 8700 8700
Size: 11110
KasI G'GCGC_C
Cuts at: 2370 5420 7497 2370
Size: 3050 2077 5983
Fragments arranged by size:
5983 3050 2077
KpnI G_GTAC'C
Cuts at: 8435 8435
Size: 11110
MfeI C'AATT_G
Cuts at: 8709 8709
Size: 11110
MluI A'CGCG_T
Cuts at: 10986 10986
Size: 11110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5307 5581 7932 10224 10408 1305
Size: 4002 274 2351 2292 184 2007
Fragments arranged by size:
4002 2351 2292 2007 274 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2849 3164 3205 3321 3694
Size: 54 1425 343 1017 315 41 116 373
Cuts at: 3694 4109 5195 5826 7010 10
Size: 415 1086 631 1184 4110
Fragments arranged by size:
4110 1425 1184 1086 1017 631 415 373
343 315 116 54 41
MslI CAynn'nnrTG
Cuts at: 518 1166 3402 3688 4775 5429 5591 9068 9427
Size: 648 2236 286 1087 654 162 3477 359
Cuts at: 9427 9586 518
Size: 159 2042
Fragments arranged by size:
3477 2236 2042 1087 654 648 359 286
162 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2194 2302 2368 2392 2843 3103 3178
Size: 570 18 108 66 24 451 260 75
Cuts at: 3178 3224 3286 3948 4316 4378 5097 5429 5517
Size: 46 62 662 368 62 719 332 88
Cuts at: 5517 6589 6621 6687 7393 8353 8381 8526 9203
Size: 1072 32 66 706 960 28 145 677
Cuts at: 9203 10351 10596 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1072 960 719 706 677 662
570 451 368 332 260 245 145 108
88 75 66 66 62 62 46 32
28 24 18
NaeI GCC'GGC
Cuts at: 2924 5017 5419 2924
Size: 2093 402 8615
Fragments arranged by size:
8615 2093 402
NarI GG'CG_CC
Cuts at: 2371 5421 7498 2371
Size: 3050 2077 5983
Fragments arranged by size:
5983 3050 2077
NcoI C'CATG_G
Cuts at: 513 1065 3159 3237 4014 4217 4497 513
Size: 552 2094 78 777 203 280 7126
Fragments arranged by size:
7126 2094 777 552 280 203 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11110
NgoMIV G'CCGG_C
Cuts at: 2922 5015 5417 2922
Size: 2093 402 8615
Fragments arranged by size:
8615 2093 402
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11110
NotI GC'GGCC_GC
Cuts at: 8557 8557
Size: 11110
NspI r_CATG'y
Cuts at: 1132 1229 5054 5300 5940 7132 7574 8468 10013
Size: 97 3825 246 640 1192 442 894 1545
Cuts at: 10013 1132
Size: 2229
Fragments arranged by size:
3825 2229 1545 1192 894 640 442 246
97
PasI CC'CwG_GG
Cuts at: 1348 2183 2318 2476 3308 4209 4490 6051 6418
Size: 835 135 158 832 901 281 1561 367
Cuts at: 6418 6449 6737 7297 1348
Size: 31 288 560 5161
Fragments arranged by size:
5161 1561 901 835 832 560 367 288
281 158 135 31
PciI A'CATG_T
Cuts at: 10009 10009
Size: 11110
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 3500 5227 5240 5833 6871 7006 8083
Size: 1374 1018 1727 13 593 1038 135 1077
Cuts at: 8083 1108
Size: 4135
Fragments arranged by size:
4135 1727 1374 1077 1038 1018 593 135
13
PfoI T'CCnGG_A
Cuts at: 2763 4833 5925 7578 8228 2763
Size: 2070 1092 1653 650 5645
Fragments arranged by size:
5645 2070 1653 1092 650
PmeI GTTT'AAAC
Cuts at: 8414 8414
Size: 11110
PmlI CAC'GTG
Cuts at: 2675 6012 6427 2675
Size: 3337 415 7358
Fragments arranged by size:
7358 3337 415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9179 9211 10692 10724 9179
Size: 32 1481 32 9565
Fragments arranged by size:
9565 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2125 2710 3172 3387 3490 4360 6046 6163
Size: 72 585 462 215 103 870 1686 117
Cuts at: 6163 6766 7315 7525 2053
Size: 603 549 210 5638
Fragments arranged by size:
5638 1686 870 603 585 549 462 215
210 117 103 72
PshAI GACnn'nnGTC
Cuts at: 1613 5870 1613
Size: 4257 6853
PsiI TTA'TAA
Cuts at: 7371 8680 7371
Size: 1309 9801
PspOMI G'GGCC_C
Cuts at: 2017 2322 2565 3304 3559 4010 4642 2017
Size: 305 243 739 255 451 632 8485
Fragments arranged by size:
8485 739 632 451 305 255 243
PspXI vC'TCGA_Gb
Cuts at: 2384 3547 4880 2384
Size: 1163 1333 8614
Fragments arranged by size:
8614 1333 1163
PstI C_TGCA'G
Cuts at: 830 2112 2193 2718 2842 3361 4414 4980 5093
Size: 1282 81 525 124 519 1053 566 113
Cuts at: 5093 5889 5960 6147 7515 7794 8462 830
Size: 796 71 187 1368 279 668 3478
Fragments arranged by size:
3478 1368 1282 1053 796 668 566 525
519 279 187 124 113 81 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11110
PvuII CAG'CTG
Cuts at: 2176 2194 2302 2392 2843 3103 3224 3286 6589
Size: 18 108 90 451 260 121 62 3303
Cuts at: 6589 8353 8381 2176
Size: 1764 28 4905
Fragments arranged by size:
4905 3303 1764 451 260 121 108 90
62 28 18
SacI G_AGCT'C
Cuts at: 3840 4989 3840
Size: 1149 9961
SacII CC_GC'GG
Cuts at: 5518 5518
Size: 11110
SalI G'TCGA_C
Cuts at: 1936 8452 1936
Size: 6516 4594
SanDI GG'GwC_CC
Cuts at: 2125 3172 3490 2125
Size: 1047 318 9745
Fragments arranged by size:
9745 1047 318
SapI GCTCTTCn'nnn_
Cuts at: 2031 4437 6539 6851 2031
Size: 2406 2102 312 6290
Fragments arranged by size:
6290 2406 2102 312
SbfI CC_TGCA'GG
Cuts at: 2718 8462 2718
Size: 5744 5366
ScaI AGT'ACT
Cuts at: 7862 8297 9358 11070 7862
Size: 435 1061 1712 7902
Fragments arranged by size:
7902 1712 1061 435
SexAI A'CCwGG_T
Cuts at: 6081 6990 7659 6081
Size: 909 669 9532
Fragments arranged by size:
9532 909 669
SfcI C'TryA_G
Cuts at: 826 1045 2108 2189 2714 2838 3357 4410 4976
Size: 219 1063 81 525 124 519 1053 566
Cuts at: 4976 5089 5247 5885 5956 6143 7511 7790 8458
Size: 113 158 638 71 187 1368 279 668
Cuts at: 8458 9593 10274 10465 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1368 1135 1063 1053 681 668 638
566 525 519 279 219 191 187 158
124 113 81 71
SfiI GGCCn_nnn'nGGCC
Cuts at: 2410 2410
Size: 11110
SfoI GGC'GCC
Cuts at: 2372 5422 7499 2372
Size: 3050 2077 5983
Fragments arranged by size:
5983 3050 2077
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11110
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11110
SmaI CCC'GGG
Cuts at: 1872 2564 3032 4699 4901 5165 8437 1872
Size: 692 468 1667 202 264 3272 4545
Fragments arranged by size:
4545 3272 1667 692 468 264 202
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 2751 3547 4637 4880 5875
Size: 197 926 441 367 796 1090 243 995
Cuts at: 5875 6950 9214 10115 10377 820
Size: 1075 2264 901 262 1553
Fragments arranged by size:
2264 1553 1090 1075 995 926 901 796
441 367 262 243 197
SnaBI TAC'GTA
Cuts at: 493 3820 8901 493
Size: 3327 5081 2702
Fragments arranged by size:
5081 3327 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11110
SphI G_CATG'C
Cuts at: 1132 8468 1132
Size: 7336 3774
SspI AAT'ATT
Cuts at: 5 52 9034 5
Size: 47 8982 2081
Fragments arranged by size:
8982 2081 47
StuI AGG'CCT
Cuts at: 1517 3932 3995 5171 6036 7696 1517
Size: 2415 63 1176 865 1660 4931
Fragments arranged by size:
4931 2415 1660 1176 865 63
StyI C'CwwG_G
Cuts at: 513 1065 3159 3237 3517 4014 4217 4497 4725
Size: 552 2094 78 280 497 203 280 228
Cuts at: 4725 5751 5944 6240 7737 8549 513
Size: 1026 193 296 1497 812 3074
Fragments arranged by size:
3074 2094 1497 1026 812 552 497 296
280 280 228 203 193 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2566 3071 3257 3339 3492 4001 4102
Size: 1255 986 505 186 82 153 509 101
Cuts at: 4102 5213 6500 6674 6761 7403 9137 9154 9307
Size: 1111 1287 174 87 642 1734 17 153
Cuts at: 9307 9492 9937 325
Size: 185 445 1498
Fragments arranged by size:
1734 1498 1287 1255 1111 986 642 509
505 445 186 185 174 153 153 101
87 82 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2643 6309 6534
Size: 274 80 33 51 1443 666 3666 225
Cuts at: 6534 7125 7860 8283 8295 9356 11068 96
Size: 591 735 423 12 1061 1712 138
Fragments arranged by size:
3666 1712 1443 1061 735 666 591 423
274 225 138 80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 3314 3327 3346 3359 4102 4134 4561
Size: 32 517 13 19 13 743 32 427
Cuts at: 4561 4593 6012 6044 7527 7559 11070 11102 2765
Size: 32 1419 32 1483 32 3511 32 2773
Fragments arranged by size:
3511 2773 1483 1419 743 517 427 32
32 32 32 32 32 19 13 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11110
XbaI T'CTAG_A
Cuts at: 2726 8446 2726
Size: 5720 5390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2180 2530 2623 3193 3198 3262 3501 4304
Size: 169 350 93 570 5 64 239 803
Cuts at: 4304 4447 5689 6247 6676 2011
Size: 143 1242 558 429 6445
Fragments arranged by size:
6445 1242 803 570 558 429 350 239
169 143 93 64 5
XhoI C'TCGA_G
Cuts at: 1943 2384 2751 3547 4880 5875 1943
Size: 441 367 796 1333 995 7178
Fragments arranged by size:
7178 1333 995 796 441 367
XmaI C'CCGG_G
Cuts at: 1870 2562 3030 4697 4899 5163 8435 1870
Size: 692 468 1667 202 264 3272 4545
Fragments arranged by size:
4545 3272 1667 692 468 264 202
XmnI GAAnn'nnTTC
Cuts at: 6632 7862 9239 6632
Size: 1230 1377 8503
Fragments arranged by size:
8503 1377 1230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8918 276
Size: 53 83 186 936 7384 2468
Fragments arranged by size:
7384 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI EcoRV FspAI
NruI NsiI PacI PsrI RsrII SrfI SwaI