Restriction map for
pFN21AA0394
(Circular) (Six-base) MAPSORT of: pFN21AA0394.seq Check: 2284 from: 1 to: 5941
pFN21AA0394 5941 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 4004 5388 5656 1632
Size: 884 1488 1384 268 1917
Fragments arranged by size:
1917 1488 1384 884 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 3114 4840 5817 1203
Size: 1911 1726 977 1327
Fragments arranged by size:
1911 1726 1327 977
AgeI A'CCGG_T
Cuts at: 2270 4772 2270
Size: 2502 3439
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 2253 1166
Size: 1087 4854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2525 1184
Size: 1341 4600
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 2466 2498 2276
Size: 32 158 32 5719
Fragments arranged by size:
5719 158 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2243 2287 2386 2389 2881 3079 3684 5256 2243
Size: 44 99 3 492 198 605 1572 2928
Fragments arranged by size:
2928 1572 605 492 198 99 44 3
ApaI G_GGCC'C
Cuts at: 2641 2641
Size: 5941
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2528 3250 3476 3628 5737 1493
Size: 1035 722 226 152 2109 1697
Fragments arranged by size:
2109 1697 1035 722 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 3266 1870
Size: 73 1323 4545
Fragments arranged by size:
4545 1323 73
BamHI G'GATC_C
Cuts at: 2291 3271 3638 2291
Size: 980 367 4594
Fragments arranged by size:
4594 980 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3262 4717 618
Size: 325 217 627 1475 1455 1842
Fragments arranged by size:
1842 1475 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2183 2641 3141 3260 1391
Size: 792 458 500 119 4072
Fragments arranged by size:
4072 792 500 458 119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 2876 928
Size: 971 666 311 3993
Fragments arranged by size:
3993 971 666 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2779 2813 4132 4166 1903
Size: 34 842 34 1319 34 3678
Fragments arranged by size:
3678 1319 842 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2198 3833 5043 2198
Size: 1635 1210 3096
Fragments arranged by size:
3096 1635 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4552 136
Size: 107 122 71 631 605 2880 1525
Fragments arranged by size:
2880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 2580 2894 3358 2580
Size: 314 464 5163
Fragments arranged by size:
5163 464 314
Bme1580I G_kGCm'C
Cuts at: 1790 2641 4002 5158 1790
Size: 851 1361 1156 2573
Fragments arranged by size:
2573 1361 1156 851
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2986 3672 4630
Size: 416 53 83 399 24 1569 686 958
Cuts at: 4630 442
Size: 1753
Fragments arranged by size:
1753 1569 958 686 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2975 2635
Size: 340 5601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2468 2882 2903 2970 3065 4601 1239
Size: 981 248 414 21 67 95 1536 2579
Fragments arranged by size:
2579 1536 981 414 248 95 67 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2666 4066 4931 5229 2666
Size: 1400 865 298 3378
Fragments arranged by size:
3378 1400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 2850 4604 882
Size: 617 612 739 1754 2219
Fragments arranged by size:
2219 1754 739 617 612
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 2296 5935 2296
Size: 3639 2302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3748 4130 4765 275
Size: 53 83 186 936 2215 382 635 1451
Fragments arranged by size:
2215 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2270 4374 4772 5046 5193 5604 2270
Size: 2104 398 274 147 411 2607
Fragments arranged by size:
2607 2104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2466 2496 1178
Size: 30 165 30 1063 30 4623
Fragments arranged by size:
4623 1063 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2924 1186
Size: 1738 4203
BseYI C'CCAG_C
Cuts at: 2135 2224 2465 2874 5144 5640 2135
Size: 89 241 409 2270 496 2436
Fragments arranged by size:
2436 2270 496 409 241 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5368
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2183 3204 4002 4087 5158 2183
Size: 1021 798 85 1071 2966
Fragments arranged by size:
2966 1071 1021 798 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 2946 2946
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2641 3141 3204 3260 4002 4087
Size: 399 393 458 500 63 56 742 85
Cuts at: 4087 5158 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 500 458 399 393 85
63 56
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2848 3040 3828 1394
Size: 429 1025 192 788 3507
Fragments arranged by size:
3507 1025 788 429 192
BsrDI GCAATG_nn'
Cuts at: 65 1248 2775 4436 4610 65
Size: 1183 1527 1661 174 1396
Fragments arranged by size:
1661 1527 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2270 4585 4772 1700
Size: 110 275 185 2315 187 2869
Fragments arranged by size:
2869 2315 275 187 185 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 3157 4745 3157
Size: 1588 4353
BstEII G'GTnAC_C
Cuts at: 1184 3016 1184
Size: 1832 4109
BstXI CCAn_nnnn'nTGG
Cuts at: 2058 2403 3028 2058
Size: 345 625 4971
Fragments arranged by size:
4971 625 345
BstYI r'GATC_y
Cuts at: 1972 2291 2974 3271 3638 4023 4040 5492 5578
Size: 319 683 297 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 683 385 367 346 319 297
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2079 2079
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2004 3141 513
Size: 552 939 1137 3313
Fragments arranged by size:
3313 1137 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2283 487
Size: 683 56 1057 4145
Fragments arranged by size:
4145 1057 683 56
BtsI GCAGTG_nn'
Cuts at: 3457 4251 4271 3457
Size: 794 20 5127
Fragments arranged by size:
5127 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3171 3388 4277
Size: 54 1425 50 275 18 1341 217 889
Cuts at: 4277 5906 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1341 889 275 217 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 3003 3871 1353
Size: 1292 358 868 3423
Fragments arranged by size:
3423 1292 868 358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2007 2675 3209 4528 4902 5048
Size: 1064 138 418 668 534 1319 374 146
Cuts at: 5048 387
Size: 1280
Fragments arranged by size:
1319 1280 1064 668 534 418 374 146
138
EcoICRI GAG'CTC
Cuts at: 2181 2181
Size: 5941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2468 2516 2882 2903 2970 3065
Size: 393 588 248 48 366 21 67 95
Cuts at: 3065 4004 4601 5388 5656 1239
Size: 939 597 787 268 1524
Fragments arranged by size:
1524 939 787 597 588 393 366 268
248 95 67 48 21
EcoO109I rG'GnC_Cy
Cuts at: 2220 2440 2220
Size: 220 5721
EcoRI G'AATT_C
Cuts at: 2528 3250 3628 2528
Size: 722 378 4841
Fragments arranged by size:
4841 722 378
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 5088 1678
Size: 3410 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2341 2373 4582 4614 4656
Size: 32 135 32 769 32 2209 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 2209 769 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2101 3285 3531 669
Size: 988 281 163 1184 246 3079
Fragments arranged by size:
3079 1184 988 281 246 163
HindIII A'AGCT_T
Cuts at: 748 2696 748
Size: 1948 3993
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2216 5055 5239 1305
Size: 911 2839 184 2007
Fragments arranged by size:
2839 2007 911 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 3899 4258 4417 518
Size: 648 1087 1646 359 159 2042
Fragments arranged by size:
2042 1646 1087 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2386 2494 2881 3097 3151 3357 4034 5182
Size: 780 108 387 216 54 206 677 1148
Cuts at: 5182 5427 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 780 677 387 245 216 206
108 54
NaeI GCC'GGC
Cuts at: 2087 2087
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 2085 2085
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3118 3299 4844 1132
Size: 97 1889 181 1545 2229
Fragments arranged by size:
2229 1889 1545 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 3114 4840 3114
Size: 1726 4215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2287 2881 1108
Size: 1179 594 4168
Fragments arranged by size:
4168 1179 594
PfoI T'CCnGG_A
Cuts at: 2535 2535
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2466 2498 4010 4042 5523 5555 2466
Size: 32 1512 32 1481 32 2852
Fragments arranged by size:
2852 1512 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 2440 2220
Size: 220 5721
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2637 2637
Size: 5941
PstI C_TGCA'G
Cuts at: 830 3293 830
Size: 2463 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2386 2494 2881 3097 3151 2386
Size: 108 387 216 54 5176
Fragments arranged by size:
5176 387 216 108 54
SacI G_AGCT'C
Cuts at: 2183 2183
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2220 2220
Size: 5941
SapI GCTCTTCn'nnn_
Cuts at: 2645 2645
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 3289 4424 5105 5296 826
Size: 219 2244 1135 681 191 1471
Fragments arranged by size:
2244 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 2270 1700
Size: 570 5371
SmaI CCC'GGG
Cuts at: 1872 3268 1872
Size: 1396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2681 4045 4946 5208 820
Size: 197 926 738 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 901 738 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2446 2598 2622 3380 513
Size: 552 939 442 152 24 758 3074
Fragments arranged by size:
3074 939 758 552 442 152 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2376 3968 3985 4138 4323 4768 325
Size: 1255 796 1592 17 153 185 445 1498
Fragments arranged by size:
1592 1498 1255 796 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3087 4187 5899
Size: 274 80 33 51 1443 1110 1100 1712
Cuts at: 5899 96
Size: 138
Fragments arranged by size:
1712 1443 1110 1100 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 3266 1870
Size: 1396 4545
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI
XcmI