Restriction map for
pFN21AA0389
(Circular) (Six-base) MAPSORT of: pFN21AA0389.seq Check: 4065 from: 1 to: 8560
pFN21AA0389 8560 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6370 278
Size: 53 83 186 936 4834 2468
Fragments arranged by size:
4834 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5903 1937
Size: 3966 4594
Acc65I G'GTAC_C
Cuts at: 3229 5881 3229
Size: 2652 5908
AclI AA'CG_TT
Cuts at: 1563 6687 7060 1563
Size: 5124 373 3063
Fragments arranged by size:
5124 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2313 2793 3063 3116 3491 4055 4467 4974
Size: 681 480 270 53 375 564 412 507
Cuts at: 4974 5774 6623 8007 8275 1632
Size: 800 849 1384 268 1917
Fragments arranged by size:
1917 1384 849 800 681 564 507 480
412 375 270 268 53
AflII C'TTAA_G
Cuts at: 820 1017 2906 820
Size: 197 1889 6474
Fragments arranged by size:
6474 1889 197
AflIII A'CryG_T
Cuts at: 1203 4152 7459 8436 1203
Size: 2949 3307 977 1327
Fragments arranged by size:
3307 2949 1327 977
AgeI A'CCGG_T
Cuts at: 7391 7391
Size: 8560
AhdI GACnn_n'nnGTC
Cuts at: 7289 7289
Size: 8560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4478 1184
Size: 3294 5266
AlwNI CAG_nnn'CTG
Cuts at: 4380 5213 5851 6303 7875 4380
Size: 833 638 452 1572 5065
Fragments arranged by size:
5065 1572 833 638 452
ApaLI G'TGCA_C
Cuts at: 2965 6617 7773 2965
Size: 3652 1156 3752
Fragments arranged by size:
3752 3652 1156
ApoI r'AATT_y
Cuts at: 1493 2259 2489 2627 3958 4056 4298 4559 4629
Size: 766 230 138 1331 98 242 261 70
Cuts at: 4629 5168 5315 5869 6095 6247 8356 1493
Size: 539 147 554 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 766 554 539 261 242
230 226 152 147 138 98 70
AseI AT'TA_AT
Cuts at: 160 1032 7114 160
Size: 872 6082 1606
Fragments arranged by size:
6082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8560
AvaI C'yCGr_G
Cuts at: 1870 1943 4598 5885 1870
Size: 73 2655 1287 4545
Fragments arranged by size:
4545 2655 1287 73
BamHI G'GATC_C
Cuts at: 4192 5890 6257 4192
Size: 1698 367 6495
Fragments arranged by size:
6495 1698 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3206 3229 5881 7336
Size: 325 217 627 242 1177 23 2652 1455
Cuts at: 7336 618
Size: 1842
Fragments arranged by size:
2652 1842 1455 1177 627 325 242 217
23
BanII G_rGCy'C
Cuts at: 1391 2660 5073 5295 5879 1391
Size: 1269 2413 222 584 4072
Fragments arranged by size:
4072 2413 1269 584 222
BbeI G_GCGC'C
Cuts at: 2033 2033
Size: 8560
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2597 4836 928
Size: 971 698 2239 4652
Fragments arranged by size:
4652 2239 971 698
BbvCI CC'TCA_GC
Cuts at: 4781 5445 4781
Size: 664 7896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6751 6785 1903
Size: 34 4814 34 3678
Fragments arranged by size:
4814 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6452 7662 6452
Size: 1210 7350
BclI T'GATC_A
Cuts at: 1548 3301 3622 4303 1548
Size: 1753 321 681 5805
Fragments arranged by size:
5805 1753 681 321
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5099 7171 136
Size: 107 122 71 631 605 3427 2072 1525
Fragments arranged by size:
3427 2072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5733 8555 5733
Size: 2822 5738
BlpI GC'TnA_GC
Cuts at: 5977 5977
Size: 8560
Bme1580I G_kGCm'C
Cuts at: 1790 2969 3209 4538 6621 7777 1790
Size: 1179 240 1329 2083 1156 2573
Fragments arranged by size:
2573 2083 1329 1179 1156 240
BmgBI CAC'GTC
Cuts at: 1318 4823 1318
Size: 3505 5055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3149 3772 6291
Size: 416 53 83 399 24 1732 623 2519
Cuts at: 6291 7249 442
Size: 958 1753
Fragments arranged by size:
2519 1753 1732 958 623 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 4008 5033 7220 1239
Size: 1236 1533 1025 2187 2579
Fragments arranged by size:
2579 2187 1533 1236 1025
Bpu10I CC'TnA_GC
Cuts at: 4781 5445 4781
Size: 664 7896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 4898 5717 6685 7550 7848 2411
Size: 2487 819 968 865 298 3123
Fragments arranged by size:
3123 2487 968 865 819 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7223 882
Size: 617 5724 2219
Fragments arranged by size:
5724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5261 6351 493
Size: 711 4057 1090 2702
Fragments arranged by size:
4057 2702 1090 711
BsaBI GATnn'nnATC
Cuts at: 8554 8554
Size: 8560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 3033 6367 6749
Size: 53 83 186 936 497 1003 3334 382
Cuts at: 6749 7384 275
Size: 635 1451
Fragments arranged by size:
3334 1451 1003 936 635 497 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5140 6993 7391 7665 7812 8223 5140
Size: 1853 398 274 147 411 5477
Fragments arranged by size:
5477 1853 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2661 2673 2691 2703 1178
Size: 30 165 30 1258 12 18 12 7035
Fragments arranged by size:
7035 1258 165 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4510 4721 4871 5000 5714 1186
Size: 3324 211 150 129 714 4032
Fragments arranged by size:
4032 3324 714 211 150 129
BseYI C'CCAG_C
Cuts at: 3612 5455 7763 8259 3612
Size: 1843 2308 496 3913
Fragments arranged by size:
3913 2308 1843 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2821 3023 1163
Size: 573 1085 202 6700
Fragments arranged by size:
6700 1085 573 202
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6010 6771 7799 1540
Size: 275 186 4009 761 1028 2301
Fragments arranged by size:
4009 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 2660 2969 4054 4403 5073 5295 5395 6621
Size: 233 309 1085 349 670 222 100 1226
Cuts at: 6621 6706 7777 2427
Size: 85 1071 3210
Fragments arranged by size:
3210 1226 1085 1071 670 349 309 233
222 100 85
BsmI GAATG_Cn'
Cuts at: 4944 5800 6071 6164 4944
Size: 856 271 93 7340
Fragments arranged by size:
7340 856 271 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 8560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 2660 2969 3209 4054 4403 4538
Size: 399 637 233 309 240 845 349 135
Cuts at: 4538 5073 5295 5395 5879 6621 6706 7777 1391
Size: 535 222 100 484 742 85 1071 2174
Fragments arranged by size:
2174 1071 845 742 637 535 484 399
349 309 240 233 222 135 100 85
BspEI T'CCGG_A
Cuts at: 8223 8223
Size: 8560
BspHI T'CATG_A
Cuts at: 4138 4311 6449 8179 4138
Size: 173 2138 1730 4519
Fragments arranged by size:
4519 2138 1730 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 5915 844
Size: 2059 3012 3489
Fragments arranged by size:
3489 3012 2059
BsrBI CCG'CTC
Cuts at: 1394 1823 6447 1394
Size: 429 4624 3507
Fragments arranged by size:
4624 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3439 7055 7229 65
Size: 1183 2191 3616 174 1396
Fragments arranged by size:
3616 2191 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2468 7204 7391 1700
Size: 110 658 4736 187 2869
Fragments arranged by size:
4736 2869 658 187 110
BsrGI T'GTAC_A
Cuts at: 96 4155 96
Size: 4059 4501
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8560
BssSI C'ACGA_G
Cuts at: 3261 4125 6620 7632 3261
Size: 864 2495 1012 4189
Fragments arranged by size:
4189 2495 1012 864
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3257 794
Size: 2463 6097
BstBI TT'CG_AA
Cuts at: 3979 4477 7364 3979
Size: 498 2887 5175
Fragments arranged by size:
5175 2887 498
BstEII G'GTnAC_C
Cuts at: 1184 4115 1184
Size: 2931 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 3571 5597 3571
Size: 2026 6534
BstYI r'GATC_y
Cuts at: 1972 2706 2926 4192 5733 5890 6257 6642 6659
Size: 734 220 1266 1541 157 367 385 17
Cuts at: 6659 8111 8197 8209 8555 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1541 1452 1266 734 385 367 346
220 157 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5045 5045
Size: 8560
BtgI C'CryG_G
Cuts at: 513 1065 2004 2944 5603 513
Size: 552 939 940 2659 3470
Fragments arranged by size:
3470 2659 940 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7821
Fragments arranged by size:
7821 683 56
BtsI GCAGTG_nn'
Cuts at: 2219 2298 3765 6076 6870 6890 2219
Size: 79 1467 2311 794 20 3889
Fragments arranged by size:
3889 2311 1467 794 79 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8525
DraI TTT'AAA
Cuts at: 2208 2826 4222 5864 2208
Size: 618 1396 1642 4904
Fragments arranged by size:
4904 1642 1396 618
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7567 809
Size: 507 6251 1802
Fragments arranged by size:
6251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4650 5151 6007
Size: 54 1425 50 275 18 2820 501 856
Cuts at: 6007 6896 8525 8
Size: 889 1629 43
Fragments arranged by size:
2820 1629 1425 889 856 501 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6007 1537
Size: 275 4195 4090
Fragments arranged by size:
4195 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 3020 4758 4848 4905 5360 6490 1353
Size: 801 866 1738 90 57 455 1130 3423
Fragments arranged by size:
3423 1738 1130 866 801 455 90 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5522 7147 7521 7667 387
Size: 1064 138 3293 640 1625 374 146 1280
Fragments arranged by size:
3293 1625 1280 1064 640 374 146 138
EcoICRI GAG'CTC
Cuts at: 2658 5071 5293 2658
Size: 2413 222 5925
Fragments arranged by size:
5925 2413 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2313 2475 2793 3063 3116 3491 4008
Size: 393 681 162 318 270 53 375 517
Cuts at: 4008 4055 4467 4974 5033 5774 6623 7220 8007
Size: 47 412 507 59 741 849 597 787
Cuts at: 8007 8275 1239
Size: 268 1524
Fragments arranged by size:
1524 849 787 741 681 597 517 507
412 393 375 318 270 268 162 59
53 47
EcoO109I rG'GnC_Cy
Cuts at: 5183 5183
Size: 8560
EcoRI G'AATT_C
Cuts at: 5869 6247 5869
Size: 378 8182
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3023 3176 3023
Size: 153 8407
FspI TGC'GCA
Cuts at: 7066 7066
Size: 8560
HaeII r_GCGC'y
Cuts at: 1678 2033 5105 7707 1678
Size: 355 3072 2602 2531
Fragments arranged by size:
3072 2602 2531 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4606 4638 4976 5008 5167
Size: 32 135 32 3034 32 338 32 159
Cuts at: 5167 5199 7201 7233 7275 7307 1373
Size: 32 2002 32 42 32 2626
Fragments arranged by size:
3034 2626 2002 338 159 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4263 5904 6150 669
Size: 988 281 2325 1641 246 3079
Fragments arranged by size:
3079 2325 1641 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2403 3005 3994 4224 748
Size: 1655 602 989 230 5084
Fragments arranged by size:
5084 1655 989 602 230
HpaI GTT'AAC
Cuts at: 4263 6150 4263
Size: 1887 6673
KasI G'GCGC_C
Cuts at: 2029 2029
Size: 8560
KpnI G_GTAC'C
Cuts at: 3233 5885 3233
Size: 2652 5908
MfeI C'AATT_G
Cuts at: 2094 2391 6159 2094
Size: 297 3768 4495
Fragments arranged by size:
4495 3768 297
MluI A'CGCG_T
Cuts at: 8436 8436
Size: 8560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2647 7674 7858 1305
Size: 1342 5027 184 2007
Fragments arranged by size:
5027 2007 1342 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4652 5153 10
Size: 54 1425 343 2820 501 3417
Fragments arranged by size:
3417 2820 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 2646 2972 3929 4460 4670 5595
Size: 648 1254 226 326 957 531 210 925
Cuts at: 5595 5678 6518 6877 7036 518
Size: 83 840 359 159 2042
Fragments arranged by size:
2042 1254 957 925 840 648 531 359
326 226 210 159 83
MspA1I CmG'CkG
Cuts at: 1606 4969 5210 5482 5976 6653 7801 8046 1606
Size: 3363 241 272 494 677 1148 245 2120
Fragments arranged by size:
3363 2120 1148 677 494 272 245 241
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 8560
NarI GG'CG_CC
Cuts at: 2030 2030
Size: 8560
NcoI C'CATG_G
Cuts at: 513 1065 2004 2944 5603 513
Size: 552 939 940 2659 3470
Fragments arranged by size:
3470 2659 940 939 552
NdeI CA'TA_TG
Cuts at: 387 2269 3810 387
Size: 1882 1541 5137
Fragments arranged by size:
5137 1882 1541
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 8560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8560
NotI GC'GGCC_GC
Cuts at: 6007 6007
Size: 8560
NspI r_CATG'y
Cuts at: 1132 1229 4156 4374 5918 7463 1132
Size: 97 2927 218 1544 1545 2229
Fragments arranged by size:
2927 2229 1545 1544 218 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8560
PciI A'CATG_T
Cuts at: 4152 7459 4152
Size: 3307 5253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3784 1108
Size: 2676 5884
PmeI GTTT'AAAC
Cuts at: 5864 5864
Size: 8560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3471 3503 6629 6661 8142 8174 3471
Size: 32 3126 32 1481 32 3857
Fragments arranged by size:
3857 3126 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5183 5183
Size: 8560
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8560
PsiI TTA'TAA
Cuts at: 3224 6130 3224
Size: 2906 5654
PstI C_TGCA'G
Cuts at: 830 2158 3643 5847 5912 830
Size: 1328 1485 2204 65 3478
Fragments arranged by size:
3478 2204 1485 1328 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8560
PvuII CAG'CTG
Cuts at: 4969 5210 4969
Size: 241 8319
SacI G_AGCT'C
Cuts at: 2660 5073 5295 2660
Size: 2413 222 5925
Fragments arranged by size:
5925 2413 222
SalI G'TCGA_C
Cuts at: 1936 5902 1936
Size: 3966 4594
SbfI CC_TGCA'GG
Cuts at: 5912 5912
Size: 8560
ScaI AGT'ACT
Cuts at: 3389 3985 6808 8520 3389
Size: 596 2823 1712 3429
Fragments arranged by size:
3429 2823 1712 596
SfcI C'TryA_G
Cuts at: 826 1045 2040 2154 3639 5843 5908 7043 7724
Size: 219 995 114 1485 2204 65 1135 681
Cuts at: 7724 7915 826
Size: 191 1471
Fragments arranged by size:
2204 1485 1471 1135 995 681 219 191
114 65
SfoI GGC'GCC
Cuts at: 2031 2031
Size: 8560
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8560
SmaI CCC'GGG
Cuts at: 1872 5887 1872
Size: 4015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 2906 4877 5696 6664 7565
Size: 197 926 483 480 1971 819 968 901
Cuts at: 7565 7827 820
Size: 262 1553
Fragments arranged by size:
1971 1553 968 926 901 819 483 480
262 197
SnaBI TAC'GTA
Cuts at: 493 6351 493
Size: 5858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8560
SphI G_CATG'C
Cuts at: 1132 5918 1132
Size: 4786 3774
SspI AAT'ATT
Cuts at: 5 52 2065 3052 3125 6484 5
Size: 47 2013 987 73 3359 2081
Fragments arranged by size:
3359 2081 2013 987 73 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2944 3043 5603 5999 513
Size: 552 939 940 99 2560 396 3074
Fragments arranged by size:
3074 2560 940 939 552 396 99
SwaI ATTT'AAAT
Cuts at: 2208 2208
Size: 8560
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5659 6587 6604 6757 6942 7387 325
Size: 1255 4079 928 17 153 185 445 1498
Fragments arranged by size:
4079 1498 1255 928 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3387 3983 4155
Size: 274 80 33 51 1443 1410 596 172
Cuts at: 4155 5191 5547 6806 8518 96
Size: 1036 356 1259 1712 138
Fragments arranged by size:
1712 1443 1410 1259 1036 596 356 274
172 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2673 2705 5710 5742 8520 8552 2673
Size: 32 3005 32 2778 32 2681
Fragments arranged by size:
3005 2778 2681 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8560
XbaI T'CTAG_A
Cuts at: 5896 5896
Size: 8560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5653 5653
Size: 8560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8560
XmaI C'CCGG_G
Cuts at: 1870 5885 1870
Size: 4015 4545
XmnI GAAnn'nnTTC
Cuts at: 2790 3441 3465 5316 6689 2790
Size: 651 24 1851 1373 4661
Fragments arranged by size:
4661 1851 1373 651 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6368 276
Size: 53 83 186 936 4834 2468
Fragments arranged by size:
4834 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SrfI