Restriction map for
pFN21AA0384
(Circular) (Six-base) MAPSORT of: pFN21AA0384.seq Check: 3096 from: 1 to: 7522
pFN21AA0384 7522 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5332 278
Size: 53 83 186 936 3796 2468
Fragments arranged by size:
3796 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2027 2761 4865 1937
Size: 90 734 2104 4594
Fragments arranged by size:
4594 2104 734 90
Acc65I G'GTAC_C
Cuts at: 4843 4843
Size: 7522
AclI AA'CG_TT
Cuts at: 1563 5649 6022 1563
Size: 4086 373 3063
Fragments arranged by size:
4086 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3432 5585 6969 7237 1632
Size: 1800 2153 1384 268 1917
Fragments arranged by size:
2153 1917 1800 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 4019 820
Size: 197 3002 4323
Fragments arranged by size:
4323 3002 197
AflIII A'CryG_T
Cuts at: 1203 2131 6421 7398 1203
Size: 928 4290 977 1327
Fragments arranged by size:
4290 1327 977 928
AgeI A'CCGG_T
Cuts at: 6353 6353
Size: 7522
AhdI GACnn_n'nnGTC
Cuts at: 2538 4098 6251 2538
Size: 1560 2153 3809
Fragments arranged by size:
3809 2153 1560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7522
AlwNI CAG_nnn'CTG
Cuts at: 5265 6837 5265
Size: 1572 5950
ApaI G_GGCC'C
Cuts at: 2781 2781
Size: 7522
ApaLI G'TGCA_C
Cuts at: 3275 5579 6735 3275
Size: 2304 1156 4062
Fragments arranged by size:
4062 2304 1156
ApoI r'AATT_y
Cuts at: 1493 2240 4309 4665 4831 5057 5209 7318 1493
Size: 747 2069 356 166 226 152 2109 1697
Fragments arranged by size:
2109 2069 1697 747 356 226 166 152
AseI AT'TA_AT
Cuts at: 160 1032 4241 6076 160
Size: 872 3209 1835 1606
Fragments arranged by size:
3209 1835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7522
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2700 3963 4847 1870
Size: 73 142 615 1263 884 4545
Fragments arranged by size:
4545 1263 884 615 142 73
AvrII C'CTAG_G
Cuts at: 2612 2612
Size: 7522
BamHI G'GATC_C
Cuts at: 2350 3916 4852 5219 2350
Size: 1566 936 367 4653
Fragments arranged by size:
4653 1566 936 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2752 2952 2989 4843
Size: 325 217 627 319 646 200 37 1854
Cuts at: 4843 6298 618
Size: 1455 1842
Fragments arranged by size:
1854 1842 1455 646 627 325 319 217
200 37
BanII G_rGCy'C
Cuts at: 1391 2781 3016 3298 3978 4841 1391
Size: 1390 235 282 680 863 4072
Fragments arranged by size:
4072 1390 863 680 282 235
BbeI G_GCGC'C
Cuts at: 2110 2956 2110
Size: 846 6676
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2447 2976 3212 3552 928
Size: 971 548 529 236 340 4898
Fragments arranged by size:
4898 971 548 529 340 236
BbvCI CC'TCA_GC
Cuts at: 4054 4054
Size: 7522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5713 5747 1903
Size: 34 3776 34 3678
Fragments arranged by size:
3776 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5414 6624 5414
Size: 1210 6312
BclI T'GATC_A
Cuts at: 1548 3507 4425 1548
Size: 1959 918 4645
Fragments arranged by size:
4645 1959 918
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2845 6133 136
Size: 107 122 71 631 605 1173 3288 1525
Fragments arranged by size:
3288 1525 1173 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7517 7517
Size: 7522
BlpI GC'TnA_GC
Cuts at: 4939 4939
Size: 7522
Bme1580I G_kGCm'C
Cuts at: 1790 2755 2781 3279 5583 6739 1790
Size: 965 26 498 2304 1156 2573
Fragments arranged by size:
2573 2304 1156 965 498 26
BmgBI CAC'GTC
Cuts at: 1318 2113 3214 3320 1318
Size: 795 1101 106 5520
Fragments arranged by size:
5520 1101 795 106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2062 2937 3019
Size: 416 53 83 399 24 645 875 82
Cuts at: 3019 3230 3846 5253 6211 442
Size: 211 616 1407 958 1753
Fragments arranged by size:
1753 1407 958 875 645 616 416 399
211 83 82 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7522
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3651 3907 3978 4050 3651
Size: 256 71 72 7123
Fragments arranged by size:
7123 256 72 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 3877 4080 4218 6182 1239
Size: 875 1763 203 138 1964 2579
Fragments arranged by size:
2579 1964 1763 875 203 138
Bpu10I CC'TnA_GC
Cuts at: 4054 4054
Size: 7522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3209 3284 5647 6512 6810 3209
Size: 75 2363 865 298 3921
Fragments arranged by size:
3921 2363 865 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2146 2327 2378 2411 4731 6185 882
Size: 617 647 181 51 33 2320 1454 2219
Fragments arranged by size:
2320 2219 1454 647 617 181 51 33
BsaAI yAC'GTr
Cuts at: 493 1204 5313 493
Size: 711 4109 2702
Fragments arranged by size:
4109 2702 711
BsaBI GATnn'nnATC
Cuts at: 2664 2820 4007 7516 2664
Size: 156 1187 3509 2670
Fragments arranged by size:
3509 2670 1187 156
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2107 2953 5329 5711
Size: 53 83 186 936 574 846 2376 382
Cuts at: 5711 6346 275
Size: 635 1451
Fragments arranged by size:
2376 1451 936 846 635 574 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3422 4626 5955 6353 6627 6774 7185 3422
Size: 1204 1329 398 274 147 411 3759
Fragments arranged by size:
3759 1329 1204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7297
Fragments arranged by size:
7297 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2360 2732 2999 3640 4291 4749 1186
Size: 925 249 372 267 641 651 458 3959
Fragments arranged by size:
3959 925 651 641 458 372 267 249
BseYI C'CCAG_C
Cuts at: 2732 3024 4082 6725 7221 2732
Size: 292 1058 2643 496 3033
Fragments arranged by size:
3033 2643 1058 496 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6949
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 2206 4763 4972 5733 6761
Size: 275 186 30 175 2557 209 761 1028
Cuts at: 6761 1540
Size: 2301
Fragments arranged by size:
2557 2301 1028 761 275 209 186 175
30
BsiHKAI G_wGCw'C
Cuts at: 3279 3298 3585 3978 4204 5583 5668 6739 3279
Size: 19 287 393 226 1379 85 1071 4062
Fragments arranged by size:
4062 1379 1071 393 287 226 85 19
BsmI GAATG_Cn'
Cuts at: 5033 5126 5033
Size: 93 7429
BsmBI CGTCTCn'nnnn_
Cuts at: 2119 2119
Size: 7522
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2755 2781 3016 3279 3298 3585 3978
Size: 399 965 26 235 263 19 287 393
Cuts at: 3978 4204 4841 5583 5668 6739 1391
Size: 226 637 742 85 1071 2174
Fragments arranged by size:
2174 1071 965 742 637 399 393 287
263 235 226 85 26 19
BspEI T'CCGG_A
Cuts at: 7185 7185
Size: 7522
BspHI T'CATG_A
Cuts at: 2958 5411 7141 2958
Size: 2453 1730 3339
Fragments arranged by size:
3339 2453 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3180 4877 844
Size: 2336 1697 3489
Fragments arranged by size:
3489 2336 1697
BsrBI CCG'CTC
Cuts at: 1394 1823 2722 3532 4113 4446 5409 1394
Size: 429 899 810 581 333 963 3507
Fragments arranged by size:
3507 963 899 810 581 429 333
BsrDI GCAATG_nn'
Cuts at: 65 1248 2569 3199 6017 6191 65
Size: 1183 1321 630 2818 174 1396
Fragments arranged by size:
2818 1396 1321 1183 630 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2190 2205 6166 6353 1700
Size: 110 380 15 3961 187 2869
Fragments arranged by size:
3961 2869 380 187 110 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7522
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7522
BssSI C'ACGA_G
Cuts at: 3393 5582 6594 3393
Size: 2189 1012 4321
Fragments arranged by size:
4321 2189 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3488 794
Size: 2694 4828
BstBI TT'CG_AA
Cuts at: 3385 6326 3385
Size: 2941 4581
BstEII G'GTnAC_C
Cuts at: 1184 2704 1184
Size: 1520 6002
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2041
Size: 7522
BstYI r'GATC_y
Cuts at: 1972 2350 2815 3783 3916 4768 4852 5219 5604
Size: 378 465 968 133 852 84 367 385
Cuts at: 5604 5621 7073 7159 7171 7517 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 968 852 465 385 378 367
346 133 86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2355 3605 2355
Size: 1250 6272
BtgI C'CryG_G
Cuts at: 513 1065 2004 2421 513
Size: 552 939 417 5614
Fragments arranged by size:
5614 939 552 417
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2232 2807 487
Size: 683 56 1006 575 5202
Fragments arranged by size:
5202 1006 683 575 56
BtsI GCAGTG_nn'
Cuts at: 2598 2683 2913 3482 5038 5832 5852 2598
Size: 85 230 569 1556 794 20 4268
Fragments arranged by size:
4268 1556 794 569 230 85 20
ClaI AT'CG_AT
Cuts at: 1554 4759 1554
Size: 3205 4317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7487
DraI TTT'AAA
Cuts at: 4826 4826
Size: 7522
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6529 809
Size: 507 5213 1802
Fragments arranged by size:
5213 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2203 4969
Size: 54 1425 50 275 18 339 34 2766
Cuts at: 4969 5858 7487 8
Size: 889 1629 43
Fragments arranged by size:
2766 1629 1425 889 339 275 54 50
43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2203 4969 1537
Size: 275 391 2766 4090
Fragments arranged by size:
4090 2766 391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5452 1353
Size: 4099 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6109 6483 6629 387
Size: 1064 138 4520 374 146 1280
Fragments arranged by size:
4520 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3296 3976 3296
Size: 680 6842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 3432 3877 4080 4218 5585 6182
Size: 393 482 1318 445 203 138 1367 597
Cuts at: 6182 6969 7237 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1367 1318 787 597 482 445 393
268 203 138
EcoO109I rG'GnC_Cy
Cuts at: 2570 2778 3074 2570
Size: 208 296 7018
Fragments arranged by size:
7018 296 208
EcoRI G'AATT_C
Cuts at: 4831 5209 4831
Size: 378 7144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3296 3296
Size: 7522
FspI TGC'GCA
Cuts at: 3065 6028 3065
Size: 2963 4559
HaeII r_GCGC'y
Cuts at: 1678 2093 2110 2956 4090 6669 1678
Size: 415 17 846 1134 2579 2531
Fragments arranged by size:
2579 2531 1134 846 415 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2492 2524 2556
Size: 32 135 32 437 32 451 32 32
Cuts at: 2556 2978 3010 3499 3531 3560 3592 4084 4116
Size: 422 32 489 32 29 32 492 32
Cuts at: 4116 6163 6195 6237 6269 1373
Size: 2047 32 42 32 2626
Fragments arranged by size:
2626 2047 492 489 451 437 422 135
42 32 32 32 32 32 32 32
32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 4866 5112 669
Size: 988 281 90 2838 246 3079
Fragments arranged by size:
3079 2838 988 281 246 90
HindIII A'AGCT_T
Cuts at: 748 3049 3725 4388 748
Size: 2301 676 663 3882
Fragments arranged by size:
3882 2301 676 663
HpaI GTT'AAC
Cuts at: 5112 5112
Size: 7522
KasI G'GCGC_C
Cuts at: 2106 2952 2106
Size: 846 6676
KpnI G_GTAC'C
Cuts at: 4847 4847
Size: 7522
MfeI C'AATT_G
Cuts at: 5121 5121
Size: 7522
MluI A'CGCG_T
Cuts at: 2131 7398 2131
Size: 5267 2255
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2903 6636 6820 1305
Size: 1598 3733 184 2007
Fragments arranged by size:
3733 2007 1598 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2171 10
Size: 54 1425 343 339 5361
Fragments arranged by size:
5361 1425 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4596 5480 5839 5998 518
Size: 648 3430 884 359 159 2042
Fragments arranged by size:
3430 2042 884 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 3164 3596 4058 4938 5615 6763 7008
Size: 519 1039 432 462 880 677 1148 245
Cuts at: 7008 1606
Size: 2120
Fragments arranged by size:
2120 1148 1039 880 677 519 462 432
245
NaeI GCC'GGC
Cuts at: 2192 2192
Size: 7522
NarI GG'CG_CC
Cuts at: 2107 2953 2107
Size: 846 6676
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6031
Fragments arranged by size:
6031 939 552
NdeI CA'TA_TG
Cuts at: 387 4597 387
Size: 4210 3312
NgoMIV G'CCGG_C
Cuts at: 2190 2190
Size: 7522
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7522
NotI GC'GGCC_GC
Cuts at: 4969 4969
Size: 7522
NspI r_CATG'y
Cuts at: 1132 1229 3491 3848 4256 4880 6425 1132
Size: 97 2262 357 408 624 1545 2229
Fragments arranged by size:
2262 2229 1545 624 408 357 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7522
PciI A'CATG_T
Cuts at: 6421 6421
Size: 7522
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2536 1108
Size: 1428 6094
PmeI GTTT'AAAC
Cuts at: 4826 4826
Size: 7522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5591 5623 7104 7136 5591
Size: 32 1481 32 5977
Fragments arranged by size:
5977 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2570 2570
Size: 7522
PshAI GACnn'nnGTC
Cuts at: 1613 2423 2946 1613
Size: 810 523 6189
Fragments arranged by size:
6189 810 523
PsiI TTA'TAA
Cuts at: 5092 5092
Size: 7522
PspOMI G'GGCC_C
Cuts at: 2777 2777
Size: 7522
PspXI vC'TCGA_Gb
Cuts at: 3963 3963
Size: 7522
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 7490
PstI C_TGCA'G
Cuts at: 830 4874 830
Size: 4044 3478
PvuI CG_AT'CG
Cuts at: 2001 4763 2001
Size: 2762 4760
PvuII CAG'CTG
Cuts at: 2125 3164 3596 4058 2125
Size: 1039 432 462 5589
Fragments arranged by size:
5589 1039 462 432
SacI G_AGCT'C
Cuts at: 3298 3978 3298
Size: 680 6842
SalI G'TCGA_C
Cuts at: 1936 2026 4864 1936
Size: 90 2838 4594
Fragments arranged by size:
4594 2838 90
SbfI CC_TGCA'GG
Cuts at: 4874 4874
Size: 7522
ScaI AGT'ACT
Cuts at: 3240 4609 5770 7482 3240
Size: 1369 1161 1712 3280
Fragments arranged by size:
3280 1712 1369 1161
SexAI A'CCwGG_T
Cuts at: 2572 2572
Size: 7522
SfcI C'TryA_G
Cuts at: 826 1045 2370 3909 4870 6005 6686 6877 826
Size: 219 1325 1539 961 1135 681 191 1471
Fragments arranged by size:
1539 1471 1325 1135 961 681 219 191
SfoI GGC'GCC
Cuts at: 2108 2954 2108
Size: 846 6676
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7522
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7522
SmaI CCC'GGG
Cuts at: 1872 2087 2702 4849 1872
Size: 215 615 2147 4545
Fragments arranged by size:
4545 2147 615 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 3299 3963 4019 5626 6527
Size: 197 926 1281 75 664 56 1607 901
Cuts at: 6527 6789 820
Size: 262 1553
Fragments arranged by size:
1607 1553 1281 926 901 664 262 197
75 56
SnaBI TAC'GTA
Cuts at: 493 5313 493
Size: 4820 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7522
SphI G_CATG'C
Cuts at: 1132 3491 4880 1132
Size: 2359 1389 3774
Fragments arranged by size:
3774 2359 1389
SspI AAT'ATT
Cuts at: 5 52 5446 5
Size: 47 5394 2081
Fragments arranged by size:
5394 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7522
StyI C'CwwG_G
Cuts at: 513 1065 2004 2612 3280 4961 513
Size: 552 939 608 668 1681 3074
Fragments arranged by size:
3074 1681 939 668 608 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5549 5566 5719 5904 6349 325
Size: 1255 3969 17 153 185 445 1498
Fragments arranged by size:
3969 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 3238 4607
Size: 274 80 33 51 1443 484 777 1369
Cuts at: 4607 5768 7480 96
Size: 1161 1712 138
Fragments arranged by size:
1712 1443 1369 1161 777 484 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7482 7514 7482
Size: 32 7490
Tth111I GACn'n_nGTC
Cuts at: 1316 2574 4325 1316
Size: 1258 1751 4513
Fragments arranged by size:
4513 1751 1258
XbaI T'CTAG_A
Cuts at: 4858 4858
Size: 7522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2164 2657 3087 2164
Size: 493 430 6599
Fragments arranged by size:
6599 493 430
XhoI C'TCGA_G
Cuts at: 1943 3963 1943
Size: 2020 5502
XmaI C'CCGG_G
Cuts at: 1870 2085 2700 4847 1870
Size: 215 615 2147 4545
Fragments arranged by size:
4545 2147 615 215
XmnI GAAnn'nnTTC
Cuts at: 4665 5651 4665
Size: 986 6536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5330 276
Size: 53 83 186 936 3796 2468
Fragments arranged by size:
3796 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I
DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI
PfoI PmlI RsrII SacII SanDI SapI SfiI SrfI
SwaI