Restriction map for
pFN21AA0382
(Circular) (Six-base) MAPSORT of: pFN21AA0382.seq Check: 9795 from: 1 to: 9337
pFN21AA0382 9337 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2704 5733 2704
Size: 3029 6308
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7147 278
Size: 53 83 186 936 5611 2468
Fragments arranged by size:
5611 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2021 6680 1937
Size: 84 4659 4594
Fragments arranged by size:
4659 4594 84
Acc65I G'GTAC_C
Cuts at: 6658 6658
Size: 9337
AclI AA'CG_TT
Cuts at: 1563 7464 7837 1563
Size: 5901 373 3063
Fragments arranged by size:
5901 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 3999 4442 4802 5021 7400 8784 9052
Size: 870 1497 443 360 219 2379 1384 268
Cuts at: 9052 1632
Size: 1917
Fragments arranged by size:
2379 1917 1497 1384 870 443 360 268
219
AfeI AGC'GCT
Cuts at: 6440 6440
Size: 9337
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9140
AflIII A'CryG_T
Cuts at: 1203 3554 5121 5226 8236 9213 1203
Size: 2351 1567 105 3010 977 1327
Fragments arranged by size:
3010 2351 1567 1327 977 105
AgeI A'CCGG_T
Cuts at: 8168 8168
Size: 9337
AhdI GACnn_n'nnGTC
Cuts at: 8066 8066
Size: 9337
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9337
AlwNI CAG_nnn'CTG
Cuts at: 2780 2858 3977 4091 5357 5519 5791 7080 8652
Size: 78 1119 114 1266 162 272 1289 1572
Cuts at: 8652 2780
Size: 3465
Fragments arranged by size:
3465 1572 1289 1266 1119 272 162 114
78
ApaLI G'TGCA_C
Cuts at: 7394 8550 7394
Size: 1156 8181
ApoI r'AATT_y
Cuts at: 1493 2625 2955 3694 4467 4494 5038 5767 6422
Size: 1132 330 739 773 27 544 729 655
Cuts at: 6422 6646 6872 7024 9133 1493
Size: 224 226 152 2109 1697
Fragments arranged by size:
2109 1697 1132 773 739 729 655 544
330 226 224 152 27
AscI GG'CGCG_CC
Cuts at: 3875 3875
Size: 9337
AseI AT'TA_AT
Cuts at: 160 1032 4380 7891 160
Size: 872 3348 3511 1606
Fragments arranged by size:
3511 3348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9337
AvaI C'yCGr_G
Cuts at: 1870 1943 6662 1870
Size: 73 4719 4545
Fragments arranged by size:
4719 4545 73
BamHI G'GATC_C
Cuts at: 6667 7034 6667
Size: 367 8970
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6034 6658 8113 618
Size: 325 217 627 4247 624 1455 1842
Fragments arranged by size:
4247 1842 1455 627 624 325 217
BanII G_rGCy'C
Cuts at: 1391 3369 3629 4408 5034 6656 1391
Size: 1978 260 779 626 1622 4072
Fragments arranged by size:
4072 1978 1622 779 626 260
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2168 2272 3364 3996 4766 5142 5838
Size: 971 269 104 1092 632 770 376 696
Cuts at: 5838 928
Size: 4427
Fragments arranged by size:
4427 1092 971 770 696 632 376 269
104
BbvCI CC'TCA_GC
Cuts at: 2745 2745
Size: 9337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7528 7562 1903
Size: 34 5591 34 3678
Fragments arranged by size:
5591 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 4465 7229 8439 3974
Size: 491 2764 1210 4872
Fragments arranged by size:
4872 2764 1210 491
BclI T'GATC_A
Cuts at: 1548 4432 4695 1548
Size: 2884 263 6190
Fragments arranged by size:
6190 2884 263
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 7948 136
Size: 107 122 71 631 605 646 5630 1525
Fragments arranged by size:
5630 1525 646 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3343 4911 9332 3343
Size: 1568 4421 3348
Fragments arranged by size:
4421 3348 1568
BlpI GC'TnA_GC
Cuts at: 6754 6754
Size: 9337
Bme1580I G_kGCm'C
Cuts at: 1790 7398 8554 1790
Size: 5608 1156 2573
Fragments arranged by size:
5608 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9337
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2879 2904 4349
Size: 416 53 83 399 24 1462 25 1445
Cuts at: 4349 6266 7068 8026 442
Size: 1917 802 958 1753
Fragments arranged by size:
1917 1753 1462 1445 958 802 416 399
83 53 25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2343 2408 2562 2794 2880 4113 4978 7997
Size: 1104 65 154 232 86 1233 865 3019
Cuts at: 7997 1239
Size: 2579
Fragments arranged by size:
3019 2579 1233 1104 865 232 154 86
65
Bpu10I CC'TnA_GC
Cuts at: 2745 2784 3363 3603 3885 5033 2745
Size: 39 579 240 282 1148 7049
Fragments arranged by size:
7049 1148 579 282 240 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3293 7462 8327 8625 3293
Size: 4169 865 298 4005
Fragments arranged by size:
4169 4005 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3814 4568 8000 882
Size: 617 2315 754 3432 2219
Fragments arranged by size:
3432 2315 2219 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 5621 7128 493
Size: 711 4417 1507 2702
Fragments arranged by size:
4417 2702 1507 711
BsaBI GATnn'nnATC
Cuts at: 3384 5554 6177 9331 3384
Size: 2170 623 3154 3390
Fragments arranged by size:
3390 3154 2170 623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2443 7144 7526 8161
Size: 53 83 186 936 910 4701 382 635
Cuts at: 8161 275
Size: 1451
Fragments arranged by size:
4701 1451 936 910 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7770 8168 8442 8589 9000 7770
Size: 398 274 147 411 8107
Fragments arranged by size:
8107 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2784 2814 3663 3693 1178
Size: 30 165 30 1381 30 849 30 6822
Fragments arranged by size:
6822 1381 849 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2143 2195 2520 4187 4649 5510 6311 6366
Size: 957 52 325 1667 462 861 801 55
Cuts at: 6366 6475 1186
Size: 109 4048
Fragments arranged by size:
4048 1667 957 861 801 462 325 109
55 52
BseYI C'CCAG_C
Cuts at: 3797 6441 8540 9036 3797
Size: 2644 2099 496 4098
Fragments arranged by size:
4098 2644 2099 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3130 3579 4187 6296 6513 1163
Size: 573 1394 449 608 2109 217 3987
Fragments arranged by size:
3987 2109 1394 608 573 449 217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6787 7548 8576 1540
Size: 275 186 4786 761 1028 2301
Fragments arranged by size:
4786 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2583 3369 3629 3705 4408 5034 6144 7398 7483
Size: 786 260 76 703 626 1110 1254 85
Cuts at: 7483 8554 2583
Size: 1071 3366
Fragments arranged by size:
3366 1254 1110 1071 786 703 626 260
85 76
BsmI GAATG_Cn'
Cuts at: 6848 6941 6848
Size: 93 9244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2583 3369 3629 3705 4408 5034 6144
Size: 399 793 786 260 76 703 626 1110
Cuts at: 6144 6656 7398 7483 8554 1391
Size: 512 742 85 1071 2174
Fragments arranged by size:
2174 1110 1071 793 786 742 703 626
512 399 260 85 76
BspEI T'CCGG_A
Cuts at: 9000 9000
Size: 9337
BspHI T'CATG_A
Cuts at: 2520 3832 5068 7226 8956 2520
Size: 1312 1236 2158 1730 2901
Fragments arranged by size:
2901 2158 1730 1312 1236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2704 3993 5733 6692 844
Size: 1860 1289 1740 959 3489
Fragments arranged by size:
3489 1860 1740 1289 959
BsrBI CCG'CTC
Cuts at: 1394 1823 3550 7224 1394
Size: 429 1727 3674 3507
Fragments arranged by size:
3674 3507 1727 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2118 5967 7832 8006 65
Size: 1183 870 3849 1865 174 1396
Fragments arranged by size:
3849 1865 1396 1183 870 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7981 8168 1700
Size: 110 6171 187 2869
Fragments arranged by size:
6171 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9337
BssHII G'CGCG_C
Cuts at: 1925 3875 1925
Size: 1950 7387
BssSI C'ACGA_G
Cuts at: 7397 8409 7397
Size: 1012 8325
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5756 794
Size: 4962 4375
BstBI TT'CG_AA
Cuts at: 4225 4414 4561 8141 4225
Size: 189 147 3580 5421
Fragments arranged by size:
5421 3580 189 147
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9337
BstXI CCAn_nnnn'nTGG
Cuts at: 3805 4308 5085 3805
Size: 503 777 8057
Fragments arranged by size:
8057 777 503
BstYI r'GATC_y
Cuts at: 1972 2189 2718 2854 3343 3379 4911 5476 6471
Size: 217 529 136 489 36 1532 565 995
Cuts at: 6471 6667 7034 7419 7436 8888 8974 8986 9332
Size: 196 367 385 17 1452 86 12 346
Cuts at: 9332 1972
Size: 1977
Fragments arranged by size:
1977 1532 1452 995 565 529 489 385
367 346 217 196 136 86 36 17
12
Bsu36I CC'TnA_GG
Cuts at: 3375 3924 3375
Size: 549 8788
BtgI C'CryG_G
Cuts at: 513 1065 3815 3858 5987 6014 6105 513
Size: 552 2750 43 2129 27 91 3745
Fragments arranged by size:
3745 2750 2129 552 91 43 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3371 487
Size: 683 56 2145 6453
Fragments arranged by size:
6453 2145 683 56
BtsI GCAGTG_nn'
Cuts at: 2896 6853 7647 7667 2896
Size: 3957 794 20 4566
Fragments arranged by size:
4566 3957 794 20
ClaI AT'CG_AT
Cuts at: 1554 3298 4587 1554
Size: 1744 1289 6304
Fragments arranged by size:
6304 1744 1289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4815 4850 529
Size: 35 4251 35 5016
Fragments arranged by size:
5016 4251 35 35
DraI TTT'AAA
Cuts at: 6641 6641
Size: 9337
DraIII CAC_nnn'GTG
Cuts at: 6170 6170
Size: 9337
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2276 3828 8344 809
Size: 507 960 1552 4516 1802
Fragments arranged by size:
4516 1802 1552 960 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4862 6784 7673
Size: 54 1425 50 275 18 3032 1922 889
Cuts at: 7673 9302 8
Size: 1629 43
Fragments arranged by size:
3032 1922 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6784 1537
Size: 275 4972 4090
Fragments arranged by size:
4972 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3781 5022 5813 6207 7267 1353
Size: 2428 1241 791 394 1060 3423
Fragments arranged by size:
3423 2428 1241 1060 791 394
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7924 8298 8444 387
Size: 1064 138 6335 374 146 1280
Fragments arranged by size:
6335 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3367 3627 4406 5032 3367
Size: 260 779 626 7672
Fragments arranged by size:
7672 779 626 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 2408 2502 2562 2794 2880 3999
Size: 393 711 65 94 60 232 86 1119
Cuts at: 3999 4113 4442 4802 4978 5021 7400 7997 8784
Size: 114 329 360 176 43 2379 597 787
Cuts at: 8784 9052 1239
Size: 268 1524
Fragments arranged by size:
2379 1524 1119 787 711 597 393 360
329 268 232 176 114 94 86 65
60 43
EcoNI CCTnn'n_nnAGG
Cuts at: 5454 5454
Size: 9337
EcoO109I rG'GnC_Cy
Cuts at: 3809 4020 5377 3809
Size: 211 1357 7769
Fragments arranged by size:
7769 1357 211
EcoRI G'AATT_C
Cuts at: 4467 6646 7024 4467
Size: 2179 378 6780
Fragments arranged by size:
6780 2179 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4535 5159 6140 4535
Size: 624 981 7732
Fragments arranged by size:
7732 981 624
FspI TGC'GCA
Cuts at: 7843 7843
Size: 9337
HaeII r_GCGC'y
Cuts at: 1678 4963 5325 6442 8484 1678
Size: 3285 362 1117 2042 2531
Fragments arranged by size:
3285 2531 2042 1117 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2174 2188 2206
Size: 32 135 32 566 32 4 14 18
Cuts at: 2206 2220 2323 2355 2515 2547 2747 2779 2944
Size: 14 103 32 160 32 200 32 165
Cuts at: 2944 2976 2996 3028 3320 3351 3352 3383 3586
Size: 32 20 32 292 31 1 31 203
Cuts at: 3586 3611 3618 3643 4046 4078 4687 4719 6167
Size: 25 7 25 403 32 609 32 1448
Cuts at: 6167 6199 6463 6495 6594 6608 6626 6640 7978
Size: 32 264 32 99 14 18 14 1338
Cuts at: 7978 8010 8052 8084 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1448 1338 609 566 403 292 264
203 200 165 160 135 103 99 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 31 31
25 25 20 18 18 14 14 14
14 7 4 1
HincII GTy'rAC
Cuts at: 669 1657 1938 5445 6205 6681 6927 669
Size: 988 281 3507 760 476 246 3079
Fragments arranged by size:
3507 3079 988 760 476 281 246
HindIII A'AGCT_T
Cuts at: 748 5289 748
Size: 4541 4796
HpaI GTT'AAC
Cuts at: 6205 6927 6205
Size: 722 8615
KpnI G_GTAC'C
Cuts at: 6662 6662
Size: 9337
MfeI C'AATT_G
Cuts at: 3846 6936 3846
Size: 3090 6247
MluI A'CGCG_T
Cuts at: 9213 9213
Size: 9337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3001 3695 4054 4815 5369 5668 8451 8635
Size: 1696 694 359 761 554 299 2783 184
Cuts at: 8635 1305
Size: 2007
Fragments arranged by size:
2783 2007 1696 761 694 554 359 299
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4864 10
Size: 54 1425 343 3032 4483
Fragments arranged by size:
4483 3032 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 3656 4802 5306 5667 5928 6083
Size: 648 1185 1305 1146 504 361 261 155
Cuts at: 6083 6171 6393 7295 7654 7813 518
Size: 88 222 902 359 159 2042
Fragments arranged by size:
2042 1305 1185 1146 902 648 504 361
359 261 222 159 155 88
MspA1I CmG'CkG
Cuts at: 1606 2749 3114 4623 5213 6753 7430 8578 8823
Size: 1143 365 1509 590 1540 677 1148 245
Cuts at: 8823 1606
Size: 2120
Fragments arranged by size:
2120 1540 1509 1148 1143 677 590 365
245
NcoI C'CATG_G
Cuts at: 513 1065 3858 6105 513
Size: 552 2793 2247 3745
Fragments arranged by size:
3745 2793 2247 552
NdeI CA'TA_TG
Cuts at: 387 2508 5940 387
Size: 2121 3432 3784
Fragments arranged by size:
3784 3432 2121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9337
NotI GC'GGCC_GC
Cuts at: 6784 6784
Size: 9337
NspI r_CATG'y
Cuts at: 1132 1229 3558 4652 6695 8240 1132
Size: 97 2329 1094 2043 1545 2229
Fragments arranged by size:
2329 2229 2043 1545 1094 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9337
PciI A'CATG_T
Cuts at: 3554 8236 3554
Size: 4682 4655
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6514 6595 1108
Size: 5406 81 3850
Fragments arranged by size:
5406 3850 81
PfoI T'CCnGG_A
Cuts at: 2857 2857
Size: 9337
PmeI GTTT'AAAC
Cuts at: 6641 6641
Size: 9337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4395 4427 5851 5883 7406 7438 8919 8951 4395
Size: 32 1424 32 1523 32 1481 32 4781
Fragments arranged by size:
4781 1523 1481 1424 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3809 5377 3809
Size: 1568 7769
PshAI GACnn'nnGTC
Cuts at: 1613 2509 1613
Size: 896 8441
PsiI TTA'TAA
Cuts at: 6907 6907
Size: 9337
PstI C_TGCA'G
Cuts at: 830 3343 4897 6324 6689 830
Size: 2513 1554 1427 365 3478
Fragments arranged by size:
3478 2513 1554 1427 365
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9337
PvuII CAG'CTG
Cuts at: 2749 3114 5213 2749
Size: 365 2099 6873
Fragments arranged by size:
6873 2099 365
SacI G_AGCT'C
Cuts at: 3369 3629 4408 5034 3369
Size: 260 779 626 7672
Fragments arranged by size:
7672 779 626 260
SalI G'TCGA_C
Cuts at: 1936 6679 1936
Size: 4743 4594
SanDI GG'GwC_CC
Cuts at: 3809 3809
Size: 9337
SapI GCTCTTCn'nnn_
Cuts at: 5022 5813 5022
Size: 791 8546
SbfI CC_TGCA'GG
Cuts at: 6689 6689
Size: 9337
ScaI AGT'ACT
Cuts at: 7585 9297 7585
Size: 1712 7625
SfcI C'TryA_G
Cuts at: 826 1045 2663 2796 2884 3339 3609 3866 4008
Size: 219 1618 133 88 455 270 257 142
Cuts at: 4008 4893 5208 6320 6685 7820 8501 8692 826
Size: 885 315 1112 365 1135 681 191 1471
Fragments arranged by size:
1618 1471 1135 1112 885 681 455 365
315 270 257 219 191 142 133 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9337
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9337
SmaI CCC'GGG
Cuts at: 1872 6664 1872
Size: 4792 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3272 7441 8342 8604 820
Size: 197 926 1329 4169 901 262 1553
Fragments arranged by size:
4169 1553 1329 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7128 493
Size: 6635 2702
SpeI A'CTAG_T
Cuts at: 152 2570 152
Size: 2418 6919
SphI G_CATG'C
Cuts at: 1132 6695 1132
Size: 5563 3774
SspI AAT'ATT
Cuts at: 5 52 4843 5911 7261 5
Size: 47 4791 1068 1350 2081
Fragments arranged by size:
4791 2081 1350 1068 47
StuI AGG'CCT
Cuts at: 1517 5918 1517
Size: 4401 4936
StyI C'CwwG_G
Cuts at: 513 1065 3480 3858 6105 6776 513
Size: 552 2415 378 2247 671 3074
Fragments arranged by size:
3074 2415 2247 671 552 378
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3543 4318 6371 7364 7381 7534 7719
Size: 1255 1963 775 2053 993 17 153 185
Cuts at: 7719 8164 325
Size: 445 1498
Fragments arranged by size:
2053 1963 1498 1255 993 775 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2326 2866 5252
Size: 274 80 33 51 1443 349 540 2386
Cuts at: 5252 6519 7583 9295 96
Size: 1267 1064 1712 138
Fragments arranged by size:
2386 1712 1443 1267 1064 540 349 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2352 2384 3663 3695 9297 9329 2352
Size: 32 1279 32 5602 32 2360
Fragments arranged by size:
5602 2360 1279 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2167 5888 1316
Size: 851 3721 4765
Fragments arranged by size:
4765 3721 851
XbaI T'CTAG_A
Cuts at: 2962 3292 3346 6673 2962
Size: 330 54 3327 5626
Fragments arranged by size:
5626 3327 330 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6596 6596
Size: 9337
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9337
XmaI C'CCGG_G
Cuts at: 1870 6662 1870
Size: 4792 4545
XmnI GAAnn'nnTTC
Cuts at: 2991 3450 4467 4515 5139 7466 2991
Size: 459 1017 48 624 2327 4862
Fragments arranged by size:
4862 2327 1017 624 459 48
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7145 276
Size: 53 83 186 936 5611 2468
Fragments arranged by size:
5611 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI