Restriction map for
pFN21AA0374
(Circular) (Six-base) MAPSORT of: pFN21AA0374.seq Check: 5166 from: 1 to: 6319
pFN21AA0374 6319 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2161 3662 1937
Size: 224 1501 4594
Fragments arranged by size:
4594 1501 224
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2435 2486 2561 3186 4382 5766 6034 1632
Size: 803 51 75 625 1196 1384 268 1917
Fragments arranged by size:
1917 1384 1196 803 625 268 75 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 3163 5048 3163
Size: 1885 4434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3087 1184
Size: 1903 4416
AlwNI CAG_nnn'CTG
Cuts at: 2362 3161 4062 5634 2362
Size: 799 901 1572 3047
Fragments arranged by size:
3047 1572 901 799
ApaI G_GGCC'C
Cuts at: 2066 2990 3210 2066
Size: 924 220 5175
Fragments arranged by size:
5175 924 220
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 3644 1870
Size: 73 150 1551 4545
Fragments arranged by size:
4545 1551 150 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 2397 2948 3177 3302
Size: 325 217 627 438 172 551 229 125
Cuts at: 3302 3436 3480 3640 5095 618
Size: 134 44 160 1455 1842
Fragments arranged by size:
1842 1455 627 551 438 325 229 217
172 160 134 125 44
BanII G_rGCy'C
Cuts at: 1391 2066 2990 3210 3270 3387 3638 1391
Size: 675 924 220 60 117 251 4072
Fragments arranged by size:
4072 924 675 251 220 117 60
BbeI G_GCGC'C
Cuts at: 3484 3484
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2474 928
Size: 971 575 4773
Fragments arranged by size:
4773 971 575
BbvCI CC'TCA_GC
Cuts at: 2086 2847 2086
Size: 761 5558
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4510 4544 1903
Size: 34 50 34 2489 34 3678
Fragments arranged by size:
3678 2489 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2091 4211 5421 2091
Size: 2120 1210 2989
Fragments arranged by size:
2989 2120 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2534 3600 4930
Size: 107 122 71 631 605 862 1066 1330
Cuts at: 4930 136
Size: 1525
Fragments arranged by size:
1525 1330 1066 862 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 2237 3575 3736 2237
Size: 1338 161 4820
Fragments arranged by size:
4820 1338 161
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2990 3182 3210 3344 3450 4380 5536
Size: 276 924 192 28 134 106 930 1156
Cuts at: 5536 1790
Size: 2573
Fragments arranged by size:
2573 1156 930 924 276 192 134 106
28
BmgBI CAC'GTC
Cuts at: 1318 2037 1318
Size: 719 5600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2736 4050 5008
Size: 416 53 83 399 24 1319 1314 958
Cuts at: 5008 442
Size: 1753
Fragments arranged by size:
1753 1319 1314 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2687 2818 3143 3418 4979 1239
Size: 1181 267 131 325 275 1561 2579
Fragments arranged by size:
2579 1561 1181 325 275 267 131
Bpu10I CC'TnA_GC
Cuts at: 2086 2847 2086
Size: 761 5558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2555 4444 5309 5607 2555
Size: 1889 865 298 3267
Fragments arranged by size:
3267 1889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2951 3207 4982 882
Size: 617 1452 256 1775 2219
Fragments arranged by size:
2219 1775 1452 617 256
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 4126 4508 5143
Size: 53 83 186 936 1948 645 382 635
Cuts at: 5143 275
Size: 1451
Fragments arranged by size:
1948 1451 936 645 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3494 3524 1178
Size: 30 165 30 2091 30 3973
Fragments arranged by size:
3973 2091 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2519 2522 2969 3368 3371 3374 1186
Size: 888 445 3 447 399 3 3 4131
Fragments arranged by size:
4131 888 447 445 399 3 3 3
BseYI C'CCAG_C
Cuts at: 2021 2529 3474 3590 3595 3612 5522 6018 2021
Size: 508 945 116 5 17 1910 496 2322
Fragments arranged by size:
2322 1910 945 508 496 116 17 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2338 2663 2951 3068 3113 3514 1163
Size: 573 602 325 288 117 45 401 3968
Fragments arranged by size:
3968 602 573 401 325 288 117 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3444 3769 4530 5558 1540
Size: 275 186 1443 325 761 1028 2301
Fragments arranged by size:
2301 1443 1028 761 325 275 186
BsiHKAI G_wGCw'C
Cuts at: 4380 4465 5536 4380
Size: 85 1071 5163
Fragments arranged by size:
5163 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
BsmBI CGTCTCn'nnnn_
Cuts at: 2663 2663
Size: 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2990 3182 3210 3270 3344 3387
Size: 399 276 924 192 28 60 74 43
Cuts at: 3387 3450 3638 4380 4465 5536 1391
Size: 63 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 924 742 399 276 192 188
85 74 63 60 43 28
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2566 3674 844
Size: 1722 1108 3489
Fragments arranged by size:
3489 1722 1108
BsrBI CCG'CTC
Cuts at: 1394 1823 2757 3471 3570 4206 1394
Size: 429 934 714 99 636 3507
Fragments arranged by size:
3507 934 714 636 429 99
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2430 2738 3439 4963 5150 1700
Size: 110 620 308 701 1524 187 2869
Fragments arranged by size:
2869 1524 701 620 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6319
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 2810 1184
Size: 1626 4693
BstXI CCAn_nnnn'nTGG
Cuts at: 3149 3149
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 3418 3649 4016 4401 4418 5870 5956 5968
Size: 1446 231 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1446 385 367 346 231 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2329 3277 3345 3369 3450 513
Size: 552 1264 948 68 24 81 3382
Fragments arranged by size:
3382 1264 948 552 81 68 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2278 2326 3234 3835 4629 4649 2278
Size: 48 908 601 794 20 3948
Fragments arranged by size:
3948 908 794 601 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 2316 2521 2316
Size: 205 6114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2476 2801 5326 809
Size: 507 1160 325 2525 1802
Fragments arranged by size:
2525 1802 1160 507 325
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2709 3319
Size: 54 1425 50 275 18 309 570 610
Cuts at: 3319 3441 3766 4655 6284 8
Size: 122 325 889 1629 43
Fragments arranged by size:
1629 1425 889 610 570 325 309 275
122 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3441 3766 1537
Size: 275 1629 325 4090
Fragments arranged by size:
4090 1629 325 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2236 3345 4249 1353
Size: 883 1109 904 3423
Fragments arranged by size:
3423 1109 904 883
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2435 2486 2561 2687 2818 3143
Size: 393 788 15 51 75 126 131 325
Cuts at: 3143 3186 3418 4382 4979 5766 6034 1239
Size: 43 232 964 597 787 268 1524
Fragments arranged by size:
1524 964 788 787 597 393 325 268
232 131 126 75 51 43 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2543 3165 2543
Size: 622 5697
EcoO109I rG'GnC_Cy
Cuts at: 2063 2745 2987 3206 3259 2063
Size: 682 242 219 53 5123
Fragments arranged by size:
5123 682 242 219 53
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2299 3484 5466 1678
Size: 621 1185 1982 2531
Fragments arranged by size:
2531 1982 1185 621
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3149 3181 3206 3238 4960
Size: 32 135 32 1577 32 25 32 1722
Cuts at: 4960 4992 5034 5066 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1722 1577 135 42 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 3480 3480
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 2711 3321 10
Size: 54 1425 343 309 570 610 3008
Fragments arranged by size:
3008 1425 610 570 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2690 4277 4636 4795 518
Size: 648 975 549 1587 359 159 2042
Fragments arranged by size:
2042 1587 975 648 549 359 159
MspA1I CmG'CkG
Cuts at: 1606 2016 2476 2533 2838 3057 3295 3478 3564
Size: 410 460 57 305 219 238 183 86
Cuts at: 3564 3599 3735 4412 5560 5805 1606
Size: 35 136 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 460 410 305 245 238
219 183 136 86 57 35
NaeI GCC'GGC
Cuts at: 3441 3441
Size: 6319
NarI GG'CG_CC
Cuts at: 3481 3481
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2329 3345 513
Size: 552 1264 1016 3487
Fragments arranged by size:
3487 1264 1016 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 3439 3439
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2488 3064 3677 5222 1132
Size: 97 1259 576 613 1545 2229
Fragments arranged by size:
2229 1545 1259 613 576 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2521 1108
Size: 1413 4906
PfoI T'CCnGG_A
Cuts at: 2436 3120 2436
Size: 684 5635
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2745 2745
Size: 6319
PshAI GACnn'nnGTC
Cuts at: 1613 2280 1613
Size: 667 5652
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 2062 2986 3206 2062
Size: 924 220 5175
Fragments arranged by size:
5175 924 220
PstI C_TGCA'G
Cuts at: 830 2376 2628 3671 830
Size: 1546 252 1043 3478
Fragments arranged by size:
3478 1546 1043 252
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 2476 2533 3057 3564 2476
Size: 57 524 507 5231
Fragments arranged by size:
5231 524 507 57
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SanDI GG'GwC_CC
Cuts at: 2745 2745
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 2633 4567 6279 2633
Size: 1934 1712 2673
Fragments arranged by size:
2673 1934 1712
SfcI C'TryA_G
Cuts at: 826 1045 2372 2624 3667 4802 5483 5674 826
Size: 219 1327 252 1043 1135 681 191 1471
Fragments arranged by size:
1471 1327 1135 1043 681 252 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2534 2534
Size: 6319
SfoI GGC'GCC
Cuts at: 3482 3482
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 2095 3646 1872
Size: 223 1551 4545
Fragments arranged by size:
4545 1551 223
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2570 4423 5324 5586 820
Size: 197 926 627 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 627 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 2488 3064 3677 1132
Size: 1356 576 613 3774
Fragments arranged by size:
3774 1356 613 576
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2291 2329 2712 3345 3758 513
Size: 552 1226 38 383 633 413 3074
Fragments arranged by size:
3074 1226 633 552 413 383 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3233 4346 4363 4516 4701 5146 325
Size: 1255 1653 1113 17 153 185 445 1498
Fragments arranged by size:
1653 1498 1255 1113 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2631 4565 6277
Size: 274 80 33 51 1443 654 1934 1712
Cuts at: 6277 96
Size: 138
Fragments arranged by size:
1934 1712 1443 654 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 2588 1316
Size: 1272 5047
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3226 3226
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 2093 3644 1870
Size: 223 1551 4545
Fragments arranged by size:
4545 1551 223
XmnI GAAnn'nnTTC
Cuts at: 2633 4448 2633
Size: 1815 4504
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SexAI SrfI SwaI