Restriction map for
pFN21AA0370
(Circular) (Six-base) MAPSORT of: pFN21AA0370.seq Check: 7258 from: 1 to: 8080
pFN21AA0370 8080 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4684 4684
Size: 8080
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2362 5890 278
Size: 53 83 186 936 826 3528 2468
Fragments arranged by size:
3528 2468 936 826 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5172 5423 1937
Size: 3235 251 4594
Fragments arranged by size:
4594 3235 251
Acc65I G'GTAC_C
Cuts at: 5401 5401
Size: 8080
AclI AA'CG_TT
Cuts at: 1563 6207 6580 1563
Size: 4644 373 3063
Fragments arranged by size:
4644 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2448 2758 3536 3585 4319 4703 6143
Size: 402 414 310 778 49 734 384 1440
Cuts at: 6143 7527 7795 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1440 1384 778 734 414 402 384
310 268 49
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7883
AflIII A'CryG_T
Cuts at: 1203 4605 6979 7956 1203
Size: 3402 2374 977 1327
Fragments arranged by size:
3402 2374 1327 977
AgeI A'CCGG_T
Cuts at: 6911 6911
Size: 8080
AhdI GACnn_n'nnGTC
Cuts at: 6809 6809
Size: 8080
AleI CACnn'nnGTG
Cuts at: 1166 3371 3761 4064 1166
Size: 2205 390 303 5182
Fragments arranged by size:
5182 2205 390 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3934 1184
Size: 2750 5330
AlwNI CAG_nnn'CTG
Cuts at: 3877 4444 5038 5104 5343 5823 7395 3877
Size: 567 594 66 239 480 1572 4562
Fragments arranged by size:
4562 1572 594 567 480 239 66
ApaLI G'TGCA_C
Cuts at: 5306 6137 7293 5306
Size: 831 1156 6093
Fragments arranged by size:
6093 1156 831
ApoI r'AATT_y
Cuts at: 1493 2054 2987 5263 5389 5615 5767 7876 1493
Size: 561 933 2276 126 226 152 2109 1697
Fragments arranged by size:
2276 2109 1697 933 561 226 152 126
AseI AT'TA_AT
Cuts at: 160 1032 6634 160
Size: 872 5602 1606
Fragments arranged by size:
5602 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8080
AvaI C'yCGr_G
Cuts at: 1870 1943 2905 4281 4402 5405 1870
Size: 73 962 1376 121 1003 4545
Fragments arranged by size:
4545 1376 1003 962 121 73
BamHI G'GATC_C
Cuts at: 5410 5777 5410
Size: 367 7713
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5401 6856 618
Size: 325 217 627 3614 1455 1842
Fragments arranged by size:
3614 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3093 3513 3680 4180 4827 4929 5399 1391
Size: 1702 420 167 500 647 102 470 4072
Fragments arranged by size:
4072 1702 647 500 470 420 167 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2462 928
Size: 971 563 6546
Fragments arranged by size:
6546 971 563
BbvCI CC'TCA_GC
Cuts at: 4905 4905
Size: 8080
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4413 4447 6271 6305 1903
Size: 34 2476 34 1824 34 3678
Fragments arranged by size:
3678 2476 1824 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4782 5972 7182 4782
Size: 1190 1210 5680
Fragments arranged by size:
5680 1210 1190
BclI T'GATC_A
Cuts at: 1548 2217 2445 4083 1548
Size: 669 228 1638 5545
Fragments arranged by size:
5545 1638 669 228
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6691 136
Size: 107 122 71 631 605 5019 1525
Fragments arranged by size:
5019 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5052 8075 5052
Size: 3023 5057
BlpI GC'TnA_GC
Cuts at: 4520 5497 4520
Size: 977 7103
Bme1580I G_kGCm'C
Cuts at: 1790 5310 6141 7297 1790
Size: 3520 831 1156 2573
Fragments arranged by size:
3520 2573 1156 831
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8080
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2673 3169 4299
Size: 416 53 83 399 24 1256 496 1130
Cuts at: 4299 4924 5811 6769 442
Size: 625 887 958 1753
Fragments arranged by size:
1753 1256 1130 958 887 625 496 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8080
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3187 4417 4810 3187
Size: 1230 393 6457
Fragments arranged by size:
6457 1230 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2146 2158 3434 4873 4955 5081 6740 1239
Size: 907 12 1276 1439 82 126 1659 2579
Fragments arranged by size:
2579 1659 1439 1276 907 126 82 12
Bpu10I CC'TnA_GC
Cuts at: 4905 4905
Size: 8080
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 6205 7070 7368 3694
Size: 2511 865 298 4406
Fragments arranged by size:
4406 2511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4054 4468 6743 882
Size: 617 2555 414 2275 2219
Fragments arranged by size:
2555 2275 2219 617 414
BsaAI yAC'GTr
Cuts at: 493 1204 5871 493
Size: 711 4667 2702
Fragments arranged by size:
4667 2702 711
BsaBI GATnn'nnATC
Cuts at: 2261 3444 4088 5144 8074 2261
Size: 1183 644 1056 2930 2267
Fragments arranged by size:
2930 2267 1183 1056 644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2359 2873 5887 6269
Size: 53 83 186 936 826 514 3014 382
Cuts at: 6269 6904 275
Size: 635 1451
Fragments arranged by size:
3014 1451 936 826 635 514 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2322 4833 5058 6513 6911 7185 7332 7743 2322
Size: 2511 225 1455 398 274 147 411 2659
Fragments arranged by size:
2659 2511 1455 411 398 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2717 2747 3515
Size: 30 165 30 741 30 543 30 768
Cuts at: 3515 3545 3662 3692 4754 4784 1178
Size: 30 117 30 1062 30 4474
Fragments arranged by size:
4474 1062 768 741 543 165 117 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2528 3008 3431 4922 5150 1186
Size: 928 414 480 423 1491 228 4116
Fragments arranged by size:
4116 1491 928 480 423 414 228
BseYI C'CCAG_C
Cuts at: 2121 2926 3408 4739 5254 7283 7779 2121
Size: 805 482 1331 515 2029 496 2422
Fragments arranged by size:
2422 2029 1331 805 515 496 482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 3638 3720 4121 4257 1163
Size: 573 828 1074 82 401 136 4986
Fragments arranged by size:
4986 1074 828 573 401 136 82
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 5530 6291 7319 1540
Size: 275 186 922 2607 761 1028 2301
Fragments arranged by size:
2607 2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3093 3360 3513 3680 4827 5310 6141 6226 7297
Size: 267 153 167 1147 483 831 85 1071
Cuts at: 7297 3093
Size: 3876
Fragments arranged by size:
3876 1147 1071 831 483 267 167 153
85
BsmI GAATG_Cn'
Cuts at: 5591 5684 5591
Size: 93 7987
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3093 3360 3513 3680 4180 4827 4929
Size: 399 1303 267 153 167 500 647 102
Cuts at: 4929 5310 5399 6141 6226 7297 1391
Size: 381 89 742 85 1071 2174
Fragments arranged by size:
2174 1303 1071 742 647 500 399 381
267 167 153 102 89 85
BspEI T'CCGG_A
Cuts at: 2322 4833 5058 7743 2322
Size: 2511 225 2685 2659
Fragments arranged by size:
2685 2659 2511 225
BspHI T'CATG_A
Cuts at: 3235 5969 7699 3235
Size: 2734 1730 3616
Fragments arranged by size:
3616 2734 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4684 5292 5334 5435 844
Size: 3840 608 42 101 3489
Fragments arranged by size:
3840 3489 608 101 42
BsrBI CCG'CTC
Cuts at: 1394 1823 5967 1394
Size: 429 4144 3507
Fragments arranged by size:
4144 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4779 6575 6749 65
Size: 1183 3531 1796 174 1396
Fragments arranged by size:
3531 1796 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2922 3645 3933 6724 6911 1700
Size: 110 1112 723 288 2791 187 2869
Fragments arranged by size:
2869 2791 1112 723 288 187 110
BsrGI T'GTAC_A
Cuts at: 96 2069 2190 3810 96
Size: 1973 121 1620 4366
Fragments arranged by size:
4366 1973 1620 121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8080
BssSI C'ACGA_G
Cuts at: 4643 6140 7152 4643
Size: 1497 1012 5571
Fragments arranged by size:
5571 1497 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2551 4659 794
Size: 1757 2108 4215
Fragments arranged by size:
4215 2108 1757
BstBI TT'CG_AA
Cuts at: 6884 6884
Size: 8080
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8080
BstXI CCAn_nnnn'nTGG
Cuts at: 2869 3505 4879 2869
Size: 636 1374 6070
Fragments arranged by size:
6070 1374 636
BstYI r'GATC_y
Cuts at: 1972 3794 4686 5052 5410 5777 6162 6179 7631
Size: 1822 892 366 358 367 385 17 1452
Cuts at: 7631 7717 7729 8075 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1822 1452 892 385 367 366 358
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2635 2635
Size: 8080
BtgI C'CryG_G
Cuts at: 513 1065 2004 3693 3975 4202 513
Size: 552 939 1689 282 227 4391
Fragments arranged by size:
4391 1689 939 552 282 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4732 5131 487
Size: 683 56 3506 399 3436
Fragments arranged by size:
3506 3436 683 399 56
BtsI GCAGTG_nn'
Cuts at: 3655 3928 4104 4238 5596 6390 6410 3655
Size: 273 176 134 1358 794 20 5325
Fragments arranged by size:
5325 1358 794 273 176 134 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8080
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8045
DraI TTT'AAA
Cuts at: 4993 5384 4993
Size: 391 7689
DraIII CAC_nnn'GTG
Cuts at: 3070 3070
Size: 8080
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7087 809
Size: 507 5771 1802
Fragments arranged by size:
5771 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2920 2937 3495
Size: 54 1425 50 275 18 1090 17 558
Cuts at: 3495 3705 4056 4205 4881 5527 6416 8045 8
Size: 210 351 149 676 646 889 1629 43
Fragments arranged by size:
1629 1425 1090 889 676 646 558 351
275 210 149 54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2920 5527 1537
Size: 275 1108 2607 4090
Fragments arranged by size:
4090 2607 1108 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2463 2777 3068 3081 5009 6010 1353
Size: 1110 314 291 13 1928 1001 3423
Fragments arranged by size:
3423 1928 1110 1001 314 291 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3647 5275 6667 7041 7187 387
Size: 1064 138 2058 1628 1392 374 146 1280
Fragments arranged by size:
2058 1628 1392 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3091 3511 3678 4825 3091
Size: 420 167 1147 6346
Fragments arranged by size:
6346 1147 420 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2146 2158 2448 2758 3434 3536
Size: 393 402 112 12 290 310 676 102
Cuts at: 3536 3585 4319 4703 4873 4955 5081 6143 6740
Size: 49 734 384 170 82 126 1062 597
Cuts at: 6740 7527 7795 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1062 787 734 676 597 402 393
384 310 290 268 170 126 112 102
82 49 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2026 3686 4643 2026
Size: 1660 957 5463
Fragments arranged by size:
5463 1660 957
EcoO109I rG'GnC_Cy
Cuts at: 2239 2491 2932 5101 5281 2239
Size: 252 441 2169 180 5038
Fragments arranged by size:
5038 2169 441 252 180
EcoRI G'AATT_C
Cuts at: 5389 5767 5389
Size: 378 7702
EcoRV GAT'ATC
Cuts at: 3262 3262
Size: 8080
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5071 5071
Size: 8080
FspI TGC'GCA
Cuts at: 6586 6586
Size: 8080
HaeII r_GCGC'y
Cuts at: 1678 7227 1678
Size: 5549 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3662 3694 4828 4860 5273
Size: 32 135 32 2090 32 1134 32 413
Cuts at: 5273 5305 6721 6753 6795 6827 1373
Size: 32 1416 32 42 32 2626
Fragments arranged by size:
2626 2090 1416 1134 413 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2881 5173 5320 5424 5670 669
Size: 988 281 943 2292 147 104 246 3079
Fragments arranged by size:
3079 2292 988 943 281 246 147 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8080
HpaI GTT'AAC
Cuts at: 2881 5670 2881
Size: 2789 5291
KpnI G_GTAC'C
Cuts at: 5405 5405
Size: 8080
MfeI C'AATT_G
Cuts at: 5679 5679
Size: 8080
MluI A'CGCG_T
Cuts at: 7956 7956
Size: 8080
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2759 3402 7194 7378 1305
Size: 1454 643 3792 184 2007
Fragments arranged by size:
3792 2007 1454 643 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2939 3497 4058 4207 4883
Size: 54 1425 343 1107 558 561 149 676
Cuts at: 4883 10
Size: 3207
Fragments arranged by size:
3207 1425 1107 676 561 558 343 149
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3761 3860 4064 6038 6397 6556
Size: 648 2205 390 99 204 1974 359 159
Cuts at: 6556 518
Size: 2042
Fragments arranged by size:
2205 2042 1974 648 390 359 204 159
99
MspA1I CmG'CkG
Cuts at: 1606 3412 3466 5220 5496 6173 7321 7566 1606
Size: 1806 54 1754 276 677 1148 245 2120
Fragments arranged by size:
2120 1806 1754 1148 677 276 245 54
NaeI GCC'GGC
Cuts at: 2924 3647 2924
Size: 723 7357
NcoI C'CATG_G
Cuts at: 513 1065 2004 3975 513
Size: 552 939 1971 4618
Fragments arranged by size:
4618 1971 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8080
NgoMIV G'CCGG_C
Cuts at: 2922 3645 2922
Size: 723 7357
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8080
NotI GC'GGCC_GC
Cuts at: 5527 5527
Size: 8080
NspI r_CATG'y
Cuts at: 1132 1229 3869 4609 5438 6983 1132
Size: 97 2640 740 829 1545 2229
Fragments arranged by size:
2640 2229 1545 829 740 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8080
PciI A'CATG_T
Cuts at: 4605 6979 4605
Size: 2374 5706
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4045 4165 5104 1108
Size: 2937 120 939 4084
Fragments arranged by size:
4084 2937 939 120
PfoI T'CCnGG_A
Cuts at: 4704 4704
Size: 8080
PmeI GTTT'AAAC
Cuts at: 5384 5384
Size: 8080
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2697 2888 2920 4916 4948 6149 6181 7662
Size: 32 191 32 1996 32 1201 32 1481
Cuts at: 7662 7694 2665
Size: 32 3051
Fragments arranged by size:
3051 1996 1481 1201 191 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2239 2491 2932 5101 5281 2239
Size: 252 441 2169 180 5038
Fragments arranged by size:
5038 2169 441 252 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8080
PsiI TTA'TAA
Cuts at: 5650 5650
Size: 8080
PstI C_TGCA'G
Cuts at: 830 3039 3690 3891 4099 4449 4695 5133 5432
Size: 2209 651 201 208 350 246 438 299
Cuts at: 5432 830
Size: 3478
Fragments arranged by size:
3478 2209 651 438 350 299 246 208
201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8080
PvuII CAG'CTG
Cuts at: 3412 5220 3412
Size: 1808 6272
SacI G_AGCT'C
Cuts at: 3093 3513 3680 4827 3093
Size: 420 167 1147 6346
Fragments arranged by size:
6346 1147 420 167
SalI G'TCGA_C
Cuts at: 1936 5171 5422 1936
Size: 3235 251 4594
Fragments arranged by size:
4594 3235 251
SanDI GG'GwC_CC
Cuts at: 2239 2239
Size: 8080
SapI GCTCTTCn'nnn_
Cuts at: 2463 3068 3081 2463
Size: 605 13 7462
Fragments arranged by size:
7462 605 13
SbfI CC_TGCA'GG
Cuts at: 4695 5432 4695
Size: 737 7343
ScaI AGT'ACT
Cuts at: 3713 6328 8040 3713
Size: 2615 1712 3753
Fragments arranged by size:
3753 2615 1712
SexAI A'CCwGG_T
Cuts at: 2538 2538
Size: 8080
SfcI C'TryA_G
Cuts at: 826 1045 3035 3686 3887 4095 4445 4691 5129
Size: 219 1990 651 201 208 350 246 438
Cuts at: 5129 5335 5428 6563 7244 7435 826
Size: 206 93 1135 681 191 1471
Fragments arranged by size:
1990 1471 1135 681 651 438 350 246
219 208 206 201 191 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8080
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8080
SmaI CCC'GGG
Cuts at: 1872 4283 5407 1872
Size: 2411 1124 4545
Fragments arranged by size:
4545 2411 1124
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3673 6184 7085 7347 820
Size: 197 926 1730 2511 901 262 1553
Fragments arranged by size:
2511 1730 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5871 493
Size: 5378 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8080
SphI G_CATG'C
Cuts at: 1132 3869 5438 1132
Size: 2737 1569 3774
Fragments arranged by size:
3774 2737 1569
SspI AAT'ATT
Cuts at: 5 52 6004 5
Size: 47 5952 2081
Fragments arranged by size:
5952 2081 47
StuI AGG'CCT
Cuts at: 1517 4548 1517
Size: 3031 5049
StyI C'CwwG_G
Cuts at: 513 1065 2004 2437 3048 3975 4005 5519 513
Size: 552 939 433 611 927 30 1514 3074
Fragments arranged by size:
3074 1514 939 927 611 552 433 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4553 5312 6107 6124 6277 6462 6907
Size: 1255 2973 759 795 17 153 185 445
Cuts at: 6907 325
Size: 1498
Fragments arranged by size:
2973 1498 1255 795 759 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 2151 2190
Size: 274 80 33 51 1443 92 82 39
Cuts at: 2190 3213 3711 3810 4555 6326 8038 96
Size: 1023 498 99 745 1771 1712 138
Fragments arranged by size:
1771 1712 1443 1023 745 498 274 138
99 92 82 80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 8040 8072 3515
Size: 32 4493 32 3523
Fragments arranged by size:
4493 3523 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8080
XbaI T'CTAG_A
Cuts at: 5416 5416
Size: 8080
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 2719 5158 2287
Size: 432 2439 5209
Fragments arranged by size:
5209 2439 432
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8080
XmaI C'CCGG_G
Cuts at: 1870 4281 5405 1870
Size: 2411 1124 4545
Fragments arranged by size:
4545 2411 1124
XmnI GAAnn'nnTTC
Cuts at: 2058 3327 4109 4455 5054 6209 2058
Size: 1269 782 346 599 1155 3929
Fragments arranged by size:
3929 1269 1155 782 599 346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2360 5888 276
Size: 53 83 186 936 826 3528 2468
Fragments arranged by size:
3528 2468 936 826 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SfiI SfoI SrfI SwaI