Restriction map for
pFN21AA0366
(Circular) (Six-base) MAPSORT of: pFN21AA0366.seq Check: 2009 from: 1 to: 8320
pFN21AA0366 8320 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5593 6130 278
Size: 53 83 186 936 4057 537 2468
Fragments arranged by size:
4057 2468 936 537 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2604 5663 1937
Size: 667 3059 4594
Fragments arranged by size:
4594 3059 667
Acc65I G'GTAC_C
Cuts at: 4185 5641 4185
Size: 1456 6864
AclI AA'CG_TT
Cuts at: 1563 6447 6820 1563
Size: 4884 373 3063
Fragments arranged by size:
4884 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 3823 4790 5310 6383 7767 8035 1632
Size: 411 1780 967 520 1073 1384 268 1917
Fragments arranged by size:
1917 1780 1384 1073 967 520 411 268
AflII C'TTAA_G
Cuts at: 820 1017 4190 820
Size: 197 3173 4950
Fragments arranged by size:
4950 3173 197
AflIII A'CryG_T
Cuts at: 1203 2220 2640 3701 4222 7219 8196 1203
Size: 1017 420 1061 521 2997 977 1327
Fragments arranged by size:
2997 1327 1061 1017 977 521 420
AgeI A'CCGG_T
Cuts at: 7151 7151
Size: 8320
AhdI GACnn_n'nnGTC
Cuts at: 7049 7049
Size: 8320
AleI CACnn'nnGTG
Cuts at: 1166 3733 3850 1166
Size: 2567 117 5636
Fragments arranged by size:
5636 2567 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2948 3126 4058 5128 1184
Size: 1764 178 932 1070 4376
Fragments arranged by size:
4376 1764 1070 932 178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3699 3731 4921 4953 3699
Size: 32 1190 32 7066
Fragments arranged by size:
7066 1190 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2496 2725 4659 5031 6063 7635 2496
Size: 229 1934 372 1032 1572 3181
Fragments arranged by size:
3181 1934 1572 1032 372 229
ApaLI G'TGCA_C
Cuts at: 3481 4838 6377 7533 3481
Size: 1357 1539 1156 4268
Fragments arranged by size:
4268 1539 1357 1156
ApoI r'AATT_y
Cuts at: 1493 2874 3680 5629 5855 6007 8116 1493
Size: 1381 806 1949 226 152 2109 1697
Fragments arranged by size:
2109 1949 1697 1381 806 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6874 160
Size: 872 5842 1606
Fragments arranged by size:
5842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
AvaI C'yCGr_G
Cuts at: 1870 1943 4868 5645 1870
Size: 73 2925 777 4545
Fragments arranged by size:
4545 2925 777 73
BamHI G'GATC_C
Cuts at: 5650 6017 5650
Size: 367 7953
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2350 4185 4823 5641 7096
Size: 325 217 627 563 1835 638 818 1455
Cuts at: 7096 618
Size: 1842
Fragments arranged by size:
1842 1835 1455 818 638 627 563 325
217
BanII G_rGCy'C
Cuts at: 1391 5639 1391
Size: 4248 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2217 4245 4379 4494 928
Size: 971 318 2028 134 115 4754
Fragments arranged by size:
4754 2028 971 318 134 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6511 6545 1903
Size: 34 4574 34 3678
Fragments arranged by size:
4574 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6212 7422 6212
Size: 1210 7110
BclI T'GATC_A
Cuts at: 1548 3388 1548
Size: 1840 6480
BfrBI ATG'CAT
Cuts at: 5345 5345
Size: 8320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6931 136
Size: 107 122 71 631 605 5259 1525
Fragments arranged by size:
5259 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3026 4339 5246 8315 3026
Size: 1313 907 3069 3031
Fragments arranged by size:
3069 3031 1313 907
BlpI GC'TnA_GC
Cuts at: 5406 5737 5406
Size: 331 7989
Bme1580I G_kGCm'C
Cuts at: 1790 3485 4842 4899 6381 7537 1790
Size: 1695 1357 57 1482 1156 2573
Fragments arranged by size:
2573 1695 1482 1357 1156 57
BmgBI CAC'GTC
Cuts at: 1318 2221 2833 1318
Size: 903 612 6805
Fragments arranged by size:
6805 903 612
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6051 7009 442
Size: 416 53 83 399 24 4634 958 1753
Fragments arranged by size:
4634 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8320
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4584 5421 4584
Size: 837 7483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 3099 3987 4948 6980 1239
Size: 888 972 888 961 2032 2579
Fragments arranged by size:
2579 2032 972 961 888 888
Bpu10I CC'TnA_GC
Cuts at: 2235 2235
Size: 8320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4422 6445 7310 7608 4422
Size: 2023 865 298 5134
Fragments arranged by size:
5134 2023 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4572 5282 6983 882
Size: 617 3073 710 1701 2219
Fragments arranged by size:
3073 2219 1701 710 617
BsaAI yAC'GTr
Cuts at: 493 1204 2403 6111 493
Size: 711 1199 3708 2702
Fragments arranged by size:
3708 2702 1199 711
BsaBI GATnn'nnATC
Cuts at: 2277 8314 2277
Size: 6037 2283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2743 3011 5590 6127
Size: 53 83 186 936 1210 268 2579 537
Cuts at: 6127 6509 7144 275
Size: 382 635 1451
Fragments arranged by size:
2579 1451 1210 936 635 537 382 268
186 83 53
BsaWI w'CCGG_w
Cuts at: 2412 6753 7151 7425 7572 7983 2412
Size: 4341 398 274 147 411 2749
Fragments arranged by size:
4341 2749 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2984 3014 4041 4071 4153
Size: 30 165 30 1581 30 1027 30 82
Cuts at: 4153 4183 4921 4951 1178
Size: 30 738 30 4547
Fragments arranged by size:
4547 1581 1027 738 165 82 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4062 4137 4242 5413 1186
Size: 2876 75 105 1171 4093
Fragments arranged by size:
4093 2876 1171 105 75
BseYI C'CCAG_C
Cuts at: 2788 2935 3006 4988 7523 8019 2788
Size: 147 71 1982 2535 496 3089
Fragments arranged by size:
3089 2535 1982 496 147 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4738 4890 1163
Size: 573 3002 152 4593
Fragments arranged by size:
4593 3002 573 152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5770 6531 7559 1540
Size: 275 186 3769 761 1028 2301
Fragments arranged by size:
3769 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2832 3485 3847 4842 5407 6381 6466 7537 2832
Size: 653 362 995 565 974 85 1071 3615
Fragments arranged by size:
3615 1071 995 974 653 565 362 85
BsmI GAATG_Cn'
Cuts at: 3105 3299 3640 3821 5831 5924 3105
Size: 194 341 181 2010 93 5501
Fragments arranged by size:
5501 2010 341 194 181 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2734 2734
Size: 8320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2832 3485 3847 4842 4899 5407 5639
Size: 399 1042 653 362 995 57 508 232
Cuts at: 5639 6381 6466 7537 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1042 995 742 653 508 399
362 232 85 57
BspEI T'CCGG_A
Cuts at: 2412 7983 2412
Size: 5571 2749
BspHI T'CATG_A
Cuts at: 3160 3391 5209 6209 7939 3160
Size: 231 1818 1000 1730 3541
Fragments arranged by size:
3541 1818 1730 1000 231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3100 4955 4963 5675 844
Size: 2256 1855 8 712 3489
Fragments arranged by size:
3489 2256 1855 712 8
BsrBI CCG'CTC
Cuts at: 1394 1823 2039 4455 4833 6207 1394
Size: 429 216 2416 378 1374 3507
Fragments arranged by size:
3507 2416 1374 429 378 216
BsrDI GCAATG_nn'
Cuts at: 65 1248 3452 3619 6815 6989 65
Size: 1183 2204 167 3196 174 1396
Fragments arranged by size:
3196 2204 1396 1183 174 167
BsrFI r'CCGG_y
Cuts at: 1700 1810 3122 4878 6964 7151 1700
Size: 110 1312 1756 2086 187 2869
Fragments arranged by size:
2869 2086 1756 1312 187 110
BsrGI T'GTAC_A
Cuts at: 96 4715 96
Size: 4619 3701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8320
BssSI C'ACGA_G
Cuts at: 6380 7392 6380
Size: 1012 7308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4851 5351 794
Size: 4057 500 3763
Fragments arranged by size:
4057 3763 500
BstBI TT'CG_AA
Cuts at: 7124 7124
Size: 8320
BstEII G'GTnAC_C
Cuts at: 1184 4859 5437 1184
Size: 3675 578 4067
Fragments arranged by size:
4067 3675 578
BstXI CCAn_nnnn'nTGG
Cuts at: 2989 5003 2989
Size: 2014 6306
BstYI r'GATC_y
Cuts at: 1972 3026 4024 4339 5246 5650 6017 6402 6419
Size: 1054 998 315 907 404 367 385 17
Cuts at: 6419 7871 7957 7969 8315 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1054 998 907 404 385 367
346 315 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2818 513
Size: 552 939 814 6015
Fragments arranged by size:
6015 939 814 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4634 487
Size: 683 56 3408 4173
Fragments arranged by size:
4173 3408 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 3857 4135 4750 5836 6630 6650 3334
Size: 523 278 615 1086 794 20 5004
Fragments arranged by size:
5004 1086 794 615 523 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3229 3264 4567 4602 4747 4782 529
Size: 35 2665 35 1303 35 145 35 4067
Fragments arranged by size:
4067 2665 1303 145 35 35 35 35
DraI TTT'AAA
Cuts at: 4299 5624 4299
Size: 1325 6995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2807 7327 809
Size: 507 1491 4520 1802
Fragments arranged by size:
4520 1802 1491 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5767 6656 8285
Size: 54 1425 50 275 18 3937 889 1629
Cuts at: 8285 8
Size: 43
Fragments arranged by size:
3937 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5767 1537
Size: 275 3955 4090
Fragments arranged by size:
4090 3955 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 4553 5329 6250 1353
Size: 1176 2024 776 921 3423
Fragments arranged by size:
3423 2024 1176 921 776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6907 7281 7427 387
Size: 1064 138 5318 374 146 1280
Fragments arranged by size:
5318 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2127 3099 3823 3987 4790 4948
Size: 393 411 84 972 724 164 803 158
Cuts at: 4948 5310 6383 6980 7767 8035 1239
Size: 362 1073 597 787 268 1524
Fragments arranged by size:
1524 1073 972 803 787 724 597 411
393 362 268 164 158 84
EcoO109I rG'GnC_Cy
Cuts at: 3566 4922 4975 3566
Size: 1356 53 6911
Fragments arranged by size:
6911 1356 53
EcoRI G'AATT_C
Cuts at: 5629 6007 5629
Size: 378 7942
FspI TGC'GCA
Cuts at: 2924 6826 2924
Size: 3902 4418
FspAI rTGC'GCAy
Cuts at: 2924 2924
Size: 8320
HaeII r_GCGC'y
Cuts at: 1678 7467 1678
Size: 5789 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2157 2189 3018 3050 4420
Size: 32 135 32 585 32 829 32 1370
Cuts at: 4420 4439 4452 4471 5207 5239 6961 6993 7035
Size: 19 13 19 736 32 1722 32 42
Cuts at: 7035 7067 1373
Size: 32 2626
Fragments arranged by size:
2626 1722 1370 829 736 585 135 42
32 32 32 32 32 32 32 19
19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 5036 5664 5910 669
Size: 988 281 3098 628 246 3079
Fragments arranged by size:
3098 3079 988 628 281 246
HindIII A'AGCT_T
Cuts at: 748 2986 4178 748
Size: 2238 1192 4890
Fragments arranged by size:
4890 2238 1192
HpaI GTT'AAC
Cuts at: 5910 5910
Size: 8320
KpnI G_GTAC'C
Cuts at: 4189 5645 4189
Size: 1456 6864
MfeI C'AATT_G
Cuts at: 3661 5919 3661
Size: 2258 6062
MluI A'CGCG_T
Cuts at: 8196 8196
Size: 8320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 2649 5618 7434 7618 1305
Size: 948 396 2969 1816 184 2007
Fragments arranged by size:
2969 2007 1816 948 396 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6498
Fragments arranged by size:
6498 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 2907 2930 3057 3125 3165 3360
Size: 648 1473 268 23 127 68 40 195
Cuts at: 3360 3733 3850 4040 4395 5001 5214 6278 6637
Size: 373 117 190 355 606 213 1064 359
Cuts at: 6637 6796 518
Size: 159 2042
Fragments arranged by size:
2042 1473 1064 648 606 373 359 355
268 213 195 190 159 127 117 68
40 23
MspA1I CmG'CkG
Cuts at: 1606 2063 2618 2725 3517 5736 6413 7561 7806
Size: 457 555 107 792 2219 677 1148 245
Cuts at: 7806 1606
Size: 2120
Fragments arranged by size:
2219 2120 1148 792 677 555 457 245
107
NaeI GCC'GGC
Cuts at: 4880 4880
Size: 8320
NcoI C'CATG_G
Cuts at: 513 1065 2004 2818 513
Size: 552 939 814 6015
Fragments arranged by size:
6015 939 814 552
NdeI CA'TA_TG
Cuts at: 387 3910 4037 5347 387
Size: 3523 127 1310 3360
Fragments arranged by size:
3523 3360 1310 127
NgoMIV G'CCGG_C
Cuts at: 4878 4878
Size: 8320
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8320
NotI GC'GGCC_GC
Cuts at: 5767 5767
Size: 8320
NsiI A_TGCA'T
Cuts at: 5347 5347
Size: 8320
NspI r_CATG'y
Cuts at: 1132 1229 2644 3130 3628 3705 4226 5678 7223
Size: 97 1415 486 498 77 521 1452 1545
Cuts at: 7223 1132
Size: 2229
Fragments arranged by size:
2229 1545 1452 1415 521 498 486 97
77
PasI CC'CwG_GG
Cuts at: 1348 4211 1348
Size: 2863 5457
PciI A'CATG_T
Cuts at: 2640 3701 4222 7219 2640
Size: 1061 521 2997 3741
Fragments arranged by size:
3741 2997 1061 521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3214 3578 1108
Size: 2106 364 5850
Fragments arranged by size:
5850 2106 364
PfoI T'CCnGG_A
Cuts at: 2470 3418 4524 2470
Size: 948 1106 6266
Fragments arranged by size:
6266 1106 948
PmeI GTTT'AAAC
Cuts at: 5624 5624
Size: 8320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2228 2260 4921 4953 6389 6421 7902 7934 2228
Size: 32 2661 32 1436 32 1481 32 2614
Fragments arranged by size:
2661 2614 1481 1436 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3566 4922 4975 3566
Size: 1356 53 6911
Fragments arranged by size:
6911 1356 53
PshAI GACnn'nnGTC
Cuts at: 1613 2144 1613
Size: 531 7789
PsiI TTA'TAA
Cuts at: 4362 5130 5890 4362
Size: 768 760 6792
Fragments arranged by size:
6792 768 760
PstI C_TGCA'G
Cuts at: 830 4985 5156 5672 830
Size: 4155 171 516 3478
Fragments arranged by size:
4155 3478 516 171
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8320
PvuII CAG'CTG
Cuts at: 2063 2618 2725 3517 2063
Size: 555 107 792 6866
Fragments arranged by size:
6866 792 555 107
SalI G'TCGA_C
Cuts at: 1936 5662 1936
Size: 3726 4594
SapI GCTCTTCn'nnn_
Cuts at: 4553 4553
Size: 8320
SbfI CC_TGCA'GG
Cuts at: 5672 5672
Size: 8320
ScaI AGT'ACT
Cuts at: 6568 8280 6568
Size: 1712 6608
SexAI A'CCwGG_T
Cuts at: 5122 5122
Size: 8320
SfcI C'TryA_G
Cuts at: 826 1045 2619 2659 4281 4981 5152 5668 6803
Size: 219 1574 40 1622 700 171 516 1135
Cuts at: 6803 7484 7675 826
Size: 681 191 1471
Fragments arranged by size:
1622 1574 1471 1135 700 681 516 219
191 171 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8320
SmaI CCC'GGG
Cuts at: 1872 5647 1872
Size: 3775 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4190 4437 6424 7325 7587 820
Size: 197 926 2247 247 1987 901 262 1553
Fragments arranged by size:
2247 1987 1553 926 901 262 247 197
SnaBI TAC'GTA
Cuts at: 493 2403 6111 493
Size: 1910 3708 2702
Fragments arranged by size:
3708 2702 1910
SpeI A'CTAG_T
Cuts at: 152 2305 152
Size: 2153 6167
SphI G_CATG'C
Cuts at: 1132 5678 1132
Size: 4546 3774
SspI AAT'ATT
Cuts at: 5 52 4705 6244 5
Size: 47 4653 1539 2081
Fragments arranged by size:
4653 2081 1539 47
StuI AGG'CCT
Cuts at: 1517 2682 1517
Size: 1165 7155
StyI C'CwwG_G
Cuts at: 513 1065 2004 2553 2818 3285 3957 5759 513
Size: 552 939 549 265 467 672 1802 3074
Fragments arranged by size:
3074 1802 939 672 552 549 467 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4847 5011 5473 6347 6364 6517 6702
Size: 1255 3267 164 462 874 17 153 185
Cuts at: 6702 7147 325
Size: 445 1498
Fragments arranged by size:
3267 1498 1255 874 462 445 185 164
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4473 4602 4715
Size: 274 80 33 51 1443 2496 129 113
Cuts at: 4715 6566 8278 96
Size: 1851 1712 138
Fragments arranged by size:
2496 1851 1712 1443 274 138 129 113
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 3574 3606 4041 4073 4950 4982 8280
Size: 32 558 32 435 32 877 32 3298
Cuts at: 8280 8312 2984
Size: 32 2992
Fragments arranged by size:
3298 2992 877 558 435 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8320
XbaI T'CTAG_A
Cuts at: 2158 5191 5656 2158
Size: 3033 465 4822
Fragments arranged by size:
4822 3033 465
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8320
XmaI C'CCGG_G
Cuts at: 1870 5645 1870
Size: 3775 4545
XmnI GAAnn'nnTTC
Cuts at: 4386 6449 4386
Size: 2063 6257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5591 6128 276
Size: 53 83 186 936 4057 537 2468
Fragments arranged by size:
4057 2468 936 537 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI KasI NarI NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI