Restriction map for
pFN21AA0365
(Circular) (Six-base) MAPSORT of: pFN21AA0365.seq Check: 4891 from: 1 to: 7951
pFN21AA0365 7951 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3544 4822 3544
Size: 1278 6673
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2175 5761 278
Size: 53 83 186 936 639 3586 2468
Fragments arranged by size:
3586 2468 936 639 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5294 1937
Size: 3357 4594
Acc65I G'GTAC_C
Cuts at: 4838 5272 4838
Size: 434 7517
AclI AA'CG_TT
Cuts at: 1563 6078 6451 1563
Size: 4515 373 3063
Fragments arranged by size:
4515 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2257 2936 3335 3617 3920 4323 6014
Size: 461 164 679 399 282 303 403 1691
Cuts at: 6014 7398 7666 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1691 1384 679 461 403 399 303
282 268 164
AflII C'TTAA_G
Cuts at: 820 1017 2131 820
Size: 197 1114 6640
Fragments arranged by size:
6640 1114 197
AflIII A'CryG_T
Cuts at: 1203 3790 6850 7827 1203
Size: 2587 3060 977 1327
Fragments arranged by size:
3060 2587 1327 977
AgeI A'CCGG_T
Cuts at: 6782 6782
Size: 7951
AhdI GACnn_n'nnGTC
Cuts at: 6680 6680
Size: 7951
AleI CACnn'nnGTG
Cuts at: 1166 4560 1166
Size: 3394 4557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7951
AlwNI CAG_nnn'CTG
Cuts at: 2704 5029 5059 5694 7266 2704
Size: 2325 30 635 1572 3389
Fragments arranged by size:
3389 2325 1572 635 30
ApaLI G'TGCA_C
Cuts at: 6008 7164 6008
Size: 1156 6795
ApoI r'AATT_y
Cuts at: 1493 2339 2414 3041 3161 3699 4487 5260 5486
Size: 846 75 627 120 538 788 773 226
Cuts at: 5486 5638 7747 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 846 788 773 627 538 226
152 120 75
AseI AT'TA_AT
Cuts at: 160 1032 6505 160
Size: 872 5473 1606
Fragments arranged by size:
5473 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7951
AvaI C'yCGr_G
Cuts at: 1870 1943 2358 3996 4968 5276 1870
Size: 73 415 1638 972 308 4545
Fragments arranged by size:
4545 1638 972 415 308 73
AvrII C'CTAG_G
Cuts at: 2680 2680
Size: 7951
BamHI G'GATC_C
Cuts at: 5281 5648 5281
Size: 367 7584
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3767 4124 4838 5272 6727
Size: 325 217 627 1980 357 714 434 1455
Cuts at: 6727 618
Size: 1842
Fragments arranged by size:
1980 1842 1455 714 627 434 357 325
217
BanII G_rGCy'C
Cuts at: 1391 2650 4050 4068 4595 4638 4667 5099 5270
Size: 1259 1400 18 527 43 29 432 171
Cuts at: 5270 1391
Size: 4072
Fragments arranged by size:
4072 1400 1259 527 432 171 43 29
18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3248 3380 3564 4358 4635 5193 928
Size: 971 1349 132 184 794 277 558 3686
Fragments arranged by size:
3686 1349 971 794 558 277 184 132
BbvCI CC'TCA_GC
Cuts at: 3961 3961
Size: 7951
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6142 6176 1903
Size: 34 4205 34 3678
Fragments arranged by size:
4205 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5843 7053 5843
Size: 1210 6741
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7951
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4779 6562 136
Size: 107 122 71 631 605 3107 1783 1525
Fragments arranged by size:
3107 1783 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2125 7946 2125
Size: 5821 2130
BlpI GC'TnA_GC
Cuts at: 4653 5368 4653
Size: 715 7236
Bme1580I G_kGCm'C
Cuts at: 1790 2807 3770 6012 7168 1790
Size: 1017 963 2242 1156 2573
Fragments arranged by size:
2573 2242 1156 1017 963
BmgBI CAC'GTC
Cuts at: 1318 2581 4536 1318
Size: 1263 1955 4733
Fragments arranged by size:
4733 1955 1263
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4220 5682 6640
Size: 416 53 83 399 24 2803 1462 958
Cuts at: 6640 442
Size: 1753
Fragments arranged by size:
2803 1753 1462 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7951
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 4931 2620
Size: 2311 5640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3614 3899 4140 4679 4752 4827 6611 1239
Size: 2375 285 241 539 73 75 1784 2579
Fragments arranged by size:
2579 2375 1784 539 285 241 75 73
Bpu10I CC'TnA_GC
Cuts at: 2612 2715 3961 4392 2612
Size: 103 1246 431 6171
Fragments arranged by size:
6171 1246 431 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2106 2448 3998 4044 6076 6941 7239 2106
Size: 342 1550 46 2032 865 298 2818
Fragments arranged by size:
2818 2032 1550 865 342 298 46
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6614 882
Size: 617 5115 2219
Fragments arranged by size:
5115 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5742 493
Size: 711 4538 2702
Fragments arranged by size:
4538 2702 711
BsaBI GATnn'nnATC
Cuts at: 7945 7945
Size: 7951
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2172 5758 6140 6775
Size: 53 83 186 936 639 3586 382 635
Cuts at: 6775 275
Size: 1451
Fragments arranged by size:
3586 1451 936 639 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2379 6384 6782 7056 7203 7614 2379
Size: 4005 398 274 147 411 2716
Fragments arranged by size:
4005 2716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4306 4336 4525 4555 1178
Size: 30 165 30 2903 30 189 30 4574
Fragments arranged by size:
4574 2903 189 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3680 3683 3954 4040 4099 4230 4697 4700
Size: 2494 3 271 86 59 131 467 3
Cuts at: 4700 4730 4822 1186
Size: 30 92 4315
Fragments arranged by size:
4315 2494 467 271 131 92 86 59
30 3 3
BseYI C'CCAG_C
Cuts at: 3741 4250 5019 7154 7650 3741
Size: 509 769 2135 496 4042
Fragments arranged by size:
4042 2135 769 509 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3993 1163
Size: 573 2257 5121
Fragments arranged by size:
5121 2257 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4147 5401 6162 7190 1540
Size: 275 186 2146 1254 761 1028 2301
Fragments arranged by size:
2301 2146 1254 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2650 2759 3135 4060 4813 5181 6012 6097 7168
Size: 109 376 925 753 368 831 85 1071
Cuts at: 7168 2650
Size: 3433
Fragments arranged by size:
3433 1071 925 831 753 376 368 109
85
BsmI GAATG_Cn'
Cuts at: 5462 5555 5462
Size: 93 7858
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2650 2759 2807 3135 3770 4050 4060
Size: 399 860 109 48 328 635 280 10
Cuts at: 4060 4068 4595 4638 4667 4813 5099 5181 5270
Size: 8 527 43 29 146 286 82 89
Cuts at: 5270 6012 6097 7168 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 860 742 635 527 399 328
286 280 146 109 89 85 82 48
43 29 10 8
BspEI T'CCGG_A
Cuts at: 7614 7614
Size: 7951
BspHI T'CATG_A
Cuts at: 5840 7570 5840
Size: 1730 6221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3544 4822 4850 5306 844
Size: 2700 1278 28 456 3489
Fragments arranged by size:
3489 2700 1278 456 28
BsrBI CCG'CTC
Cuts at: 1394 1823 4727 5838 1394
Size: 429 2904 1111 3507
Fragments arranged by size:
3507 2904 1111 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3352 6446 6620 65
Size: 1183 2104 3094 174 1396
Fragments arranged by size:
3094 2104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3306 4146 5122 6595 6782 1700
Size: 110 1496 840 976 1473 187 2869
Fragments arranged by size:
2869 1496 1473 976 840 187 110
BsrGI T'GTAC_A
Cuts at: 96 5226 96
Size: 5130 2821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7951
BssSI C'ACGA_G
Cuts at: 6011 7023 6011
Size: 1012 6939
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3971 794
Size: 3177 4774
BstBI TT'CG_AA
Cuts at: 3555 4948 6755 3555
Size: 1393 1807 4751
Fragments arranged by size:
4751 1807 1393
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7951
BstXI CCAn_nnnn'nTGG
Cuts at: 2183 2372 5008 2183
Size: 189 2636 5126
Fragments arranged by size:
5126 2636 189
BstYI r'GATC_y
Cuts at: 1972 2125 2147 2204 2332 2525 2895 2911 5281
Size: 153 22 57 128 193 370 16 2370
Cuts at: 5281 5648 6033 6050 7502 7588 7600 7946 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2370 1977 1452 385 370 367 346 193
153 128 86 57 22 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 4120 4242 4264 4680 4834 4120
Size: 122 22 416 154 7237
Fragments arranged by size:
7237 416 154 122 22
BtgI C'CryG_G
Cuts at: 513 1065 2004 2425 4031 4141 4575 4596 4643
Size: 552 939 421 1606 110 434 21 47
Cuts at: 4643 5086 513
Size: 443 3378
Fragments arranged by size:
3378 1606 939 552 443 434 421 110
47 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2196 487
Size: 683 56 970 6242
Fragments arranged by size:
6242 970 683 56
BtsI GCAGTG_nn'
Cuts at: 3981 5467 6261 6281 3981
Size: 1486 794 20 5651
Fragments arranged by size:
5651 1486 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7951
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2305 2340 3553 3588 529
Size: 35 1741 35 1213 35 4892
Fragments arranged by size:
4892 1741 1213 35 35 35
DraI TTT'AAA
Cuts at: 2116 3168 3213 5255 2116
Size: 1052 45 2042 4812
Fragments arranged by size:
4812 2042 1052 45
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6958 809
Size: 507 5642 1802
Fragments arranged by size:
5642 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2326 2374 2449
Size: 54 1425 50 275 18 496 48 75
Cuts at: 2449 4144 5398 6287 7916 8
Size: 1695 1254 889 1629 43
Fragments arranged by size:
1695 1629 1425 1254 889 496 275 75
54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4144 5398 1537
Size: 275 2332 1254 4090
Fragments arranged by size:
4090 2332 1254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2227 3311 3481 3834 4324 4850 5881
Size: 759 115 1084 170 353 490 526 1031
Cuts at: 5881 1353
Size: 3423
Fragments arranged by size:
3423 1084 1031 759 526 490 353 170
115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6538 6912 7058 387
Size: 1064 138 4949 374 146 1280
Fragments arranged by size:
4949 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2648 2648
Size: 7951
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2257 2936 3335 3614 3617 3899
Size: 393 461 164 679 399 279 3 282
Cuts at: 3899 3920 4140 4323 4679 4752 4827 6014 6611
Size: 21 220 183 356 73 75 1187 597
Cuts at: 6611 7398 7666 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1187 787 679 597 461 399 393
356 282 279 268 220 183 164 75
73 21 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2684 4166 4960 5105 2684
Size: 1482 794 145 5530
Fragments arranged by size:
5530 1482 794 145
EcoO109I rG'GnC_Cy
Cuts at: 2264 2701 3841 4101 4246 4720 2264
Size: 437 1140 260 145 474 5495
Fragments arranged by size:
5495 1140 474 437 260 145
EcoRI G'AATT_C
Cuts at: 5260 5638 5260
Size: 378 7573
EcoRV GAT'ATC
Cuts at: 2069 2875 2069
Size: 806 7145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2304 2965 3326 2304
Size: 661 361 6929
Fragments arranged by size:
6929 661 361
FspI TGC'GCA
Cuts at: 6457 6457
Size: 7951
HaeII r_GCGC'y
Cuts at: 1678 7098 1678
Size: 5420 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 2510 2542 3612
Size: 32 135 32 609 32 297 32 1070
Cuts at: 3612 3644 4281 4306 4313 4338 4392 4424 4882
Size: 32 637 25 7 25 54 32 458
Cuts at: 4882 4897 4914 4929 6592 6624 6666 6698 1373
Size: 15 17 15 1663 32 42 32 2626
Fragments arranged by size:
2626 1663 1070 637 609 458 297 135
54 42 32 32 32 32 32 32
32 32 25 25 17 15 15 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 5295 5541 669
Size: 988 281 724 2633 246 3079
Fragments arranged by size:
3079 2633 988 724 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7951
HpaI GTT'AAC
Cuts at: 5541 5541
Size: 7951
KpnI G_GTAC'C
Cuts at: 4842 5276 4842
Size: 434 7517
MfeI C'AATT_G
Cuts at: 5550 5550
Size: 7951
MluI A'CGCG_T
Cuts at: 7827 7827
Size: 7951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4210 5230 7065 7249 1305
Size: 2905 1020 1835 184 2007
Fragments arranged by size:
2905 2007 1835 1020 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2376 2451 10
Size: 54 1425 343 544 75 5510
Fragments arranged by size:
5510 1425 544 343 75 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2076 2181 3112 4346 4560 5909 6268
Size: 648 910 105 931 1234 214 1349 359
Cuts at: 6268 6427 518
Size: 159 2042
Fragments arranged by size:
2042 1349 1234 931 910 648 359 214
159 105
MspA1I CmG'CkG
Cuts at: 1606 3618 3919 4039 4645 5367 6044 7192 7437
Size: 2012 301 120 606 722 677 1148 245
Cuts at: 7437 1606
Size: 2120
Fragments arranged by size:
2120 2012 1148 722 677 606 301 245
120
NaeI GCC'GGC
Cuts at: 4148 4148
Size: 7951
NcoI C'CATG_G
Cuts at: 513 1065 2004 5086 513
Size: 552 939 3082 3378
Fragments arranged by size:
3378 3082 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7951
NgoMIV G'CCGG_C
Cuts at: 4146 4146
Size: 7951
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7951
NotI GC'GGCC_GC
Cuts at: 5398 5398
Size: 7951
NspI r_CATG'y
Cuts at: 1132 1229 3220 5309 6854 1132
Size: 97 1991 2089 1545 2229
Fragments arranged by size:
2229 2089 1991 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2848 2861 1348
Size: 1500 13 6438
Fragments arranged by size:
6438 1500 13
PciI A'CATG_T
Cuts at: 6850 6850
Size: 7951
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5092 1108
Size: 3984 3967
PfoI T'CCnGG_A
Cuts at: 4729 4920 4729
Size: 191 7760
PmeI GTTT'AAAC
Cuts at: 5255 5255
Size: 7951
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6020 6052 7533 7565 6020
Size: 32 1481 32 6406
Fragments arranged by size:
6406 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3841 4101 4246 3841
Size: 260 145 7546
Fragments arranged by size:
7546 260 145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7951
PsiI TTA'TAA
Cuts at: 5521 5521
Size: 7951
PstI C_TGCA'G
Cuts at: 830 2509 4369 5064 5303 830
Size: 1679 1860 695 239 3478
Fragments arranged by size:
3478 1860 1679 695 239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7951
SacI G_AGCT'C
Cuts at: 2650 2650
Size: 7951
SacII CC_GC'GG
Cuts at: 4646 4646
Size: 7951
SalI G'TCGA_C
Cuts at: 1936 5293 1936
Size: 3357 4594
SanDI GG'GwC_CC
Cuts at: 4101 4101
Size: 7951
SbfI CC_TGCA'GG
Cuts at: 5303 5303
Size: 7951
ScaI AGT'ACT
Cuts at: 6199 7911 6199
Size: 1712 6239
SexAI A'CCwGG_T
Cuts at: 2364 2364
Size: 7951
SfcI C'TryA_G
Cuts at: 826 1045 2208 2505 4365 5060 5299 6434 7115
Size: 219 1163 297 1860 695 239 1135 681
Cuts at: 7115 7306 826
Size: 191 1471
Fragments arranged by size:
1860 1471 1163 1135 695 681 297 239
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7951
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7951
SmaI CCC'GGG
Cuts at: 1872 3998 5278 1872
Size: 2126 1280 4545
Fragments arranged by size:
4545 2126 1280
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2121 2131 2463 4013 4059 6055
Size: 197 926 178 10 332 1550 46 1996
Cuts at: 6055 6956 7218 820
Size: 901 262 1553
Fragments arranged by size:
1996 1553 1550 926 901 332 262 197
178 46 10
SnaBI TAC'GTA
Cuts at: 493 5742 493
Size: 5249 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7951
SphI G_CATG'C
Cuts at: 1132 5309 1132
Size: 4177 3774
SspI AAT'ATT
Cuts at: 5 52 3890 5875 5
Size: 47 3838 1985 2081
Fragments arranged by size:
3838 2081 1985 47
StuI AGG'CCT
Cuts at: 1517 2241 3602 4161 4829 4966 1517
Size: 724 1361 559 668 137 4502
Fragments arranged by size:
4502 1361 724 668 559 137
StyI C'CwwG_G
Cuts at: 513 1065 2004 2680 3468 3708 4206 5001 5086
Size: 552 939 676 788 240 498 795 85
Cuts at: 5086 5390 513
Size: 304 3074
Fragments arranged by size:
3074 939 795 788 676 552 498 304
240 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3205 5048 5150 5978 5995 6148 6333
Size: 1255 1625 1843 102 828 17 153 185
Cuts at: 6333 6778 325
Size: 445 1498
Fragments arranged by size:
1843 1625 1498 1255 828 445 185 153
102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3285 3582 3990
Size: 274 80 33 51 1443 1308 297 408
Cuts at: 3990 5226 6197 7909 96
Size: 1236 971 1712 138
Fragments arranged by size:
1712 1443 1308 1236 971 408 297 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2818 2850 4525 4557 7911 7943 2818
Size: 32 1675 32 3354 32 2826
Fragments arranged by size:
3354 2826 1675 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2366 1316
Size: 1050 6901
XbaI T'CTAG_A
Cuts at: 2219 5287 2219
Size: 3068 4883
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7951
XmaI C'CCGG_G
Cuts at: 1870 3996 5276 1870
Size: 2126 1280 4545
Fragments arranged by size:
4545 2126 1280
XmnI GAAnn'nnTTC
Cuts at: 2106 4944 6080 2106
Size: 2838 1136 3977
Fragments arranged by size:
3977 2838 1136
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2173 5759 276
Size: 53 83 186 936 639 3586 2468
Fragments arranged by size:
3586 2468 936 639 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SapI SfiI SfoI SrfI SwaI XcmI