Restriction map for
pFN21AA0354
(Circular) (Six-base) MAPSORT of: pFN21AA0354.seq Check: 6500 from: 1 to: 6736
pFN21AA0354 6736 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2328 3359 2328
Size: 1031 5705
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4546 278
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2258 4079 1937
Size: 321 1821 4594
Fragments arranged by size:
4594 1821 321
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2658 3468 3956 4799 6183 6451 1632
Size: 1026 810 488 843 1384 268 1917
Fragments arranged by size:
1917 1384 1026 843 810 488 268
AfeI AGC'GCT
Cuts at: 2429 2429
Size: 6736
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 5635 6612 1203
Size: 4432 977 1327
Fragments arranged by size:
4432 1327 977
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 3900 3912 1166
Size: 2734 12 3990
Fragments arranged by size:
3990 2734 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6736
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 2019
Size: 32 6704
AlwNI CAG_nnn'CTG
Cuts at: 2044 2449 3359 4479 6051 2044
Size: 405 910 1120 1572 2729
Fragments arranged by size:
2729 1572 1120 910 405
ApaI G_GGCC'C
Cuts at: 3378 3378
Size: 6736
ApaLI G'TGCA_C
Cuts at: 4793 5949 4793
Size: 1156 5580
ApoI r'AATT_y
Cuts at: 1493 3235 4045 4271 4423 6532 1493
Size: 1742 810 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 810 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5290 160
Size: 872 4258 1606
Fragments arranged by size:
4258 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 4061 1870
Size: 73 745 1373 4545
Fragments arranged by size:
4545 1373 745 73
BamHI G'GATC_C
Cuts at: 4066 4433 4066
Size: 367 6369
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 4057 5512 618
Size: 325 217 627 1031 1239 1455 1842
Fragments arranged by size:
1842 1455 1239 1031 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2158 2471 2522 3011 3378 3656 3835 4055
Size: 767 313 51 489 367 278 179 220
Cuts at: 4055 1391
Size: 4072
Fragments arranged by size:
4072 767 489 367 313 278 220 179
51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5765
BbvCI CC'TCA_GC
Cuts at: 3113 3113
Size: 6736
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 4927 4961 1903
Size: 34 2078 34 878 34 3678
Fragments arranged by size:
3678 2078 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 2785 2902 1548
Size: 1237 117 5382
Fragments arranged by size:
5382 1237 117
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2493 5347 136
Size: 107 122 71 631 605 821 2854 1525
Fragments arranged by size:
2854 1525 821 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6731 6731
Size: 6736
BlpI GC'TnA_GC
Cuts at: 2470 4153 2470
Size: 1683 5053
Bme1580I G_kGCm'C
Cuts at: 1790 2823 3378 4797 5953 1790
Size: 1033 555 1419 1156 2573
Fragments arranged by size:
2573 1419 1156 1033 555
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6736
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2385 3145 4467
Size: 416 53 83 399 24 968 760 1322
Cuts at: 4467 5425 442
Size: 958 1753
Fragments arranged by size:
1753 1322 968 958 760 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3344 3541 5396 1239
Size: 2105 197 1855 2579
Fragments arranged by size:
2579 2105 1855 197
Bpu10I CC'TnA_GC
Cuts at: 3015 3113 3015
Size: 98 6638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2787 4861 5726 6024 2787
Size: 2074 865 298 3499
Fragments arranged by size:
3499 2074 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5399 882
Size: 617 3900 2219
Fragments arranged by size:
3900 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4527 493
Size: 711 3323 2702
Fragments arranged by size:
3323 2702 711
BsaBI GATnn'nnATC
Cuts at: 6730 6730
Size: 6736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2569 4543 4925 5560
Size: 53 83 186 936 1036 1974 382 635
Cuts at: 5560 275
Size: 1451
Fragments arranged by size:
1974 1451 1036 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2659 5169 5567 5841 5988 6399 2659
Size: 2510 398 274 147 411 2996
Fragments arranged by size:
2996 2510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3075 3105 1178
Size: 30 165 30 1672 30 4809
Fragments arranged by size:
4809 1672 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2612 2708 3370 3513 3538 3600 1186
Size: 1327 99 96 662 143 25 62 4322
Fragments arranged by size:
4322 1327 662 143 99 96 62 25
BseYI C'CCAG_C
Cuts at: 2275 2449 2822 3359 3735 4022 5939 6435 2275
Size: 174 373 537 376 287 1917 496 2576
Fragments arranged by size:
2576 1917 537 496 376 373 287 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2223 2898 3497 1163
Size: 573 487 675 599 4402
Fragments arranged by size:
4402 675 599 573 487
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4186 4947 5975 1540
Size: 275 186 2185 761 1028 2301
Fragments arranged by size:
2301 2185 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2471 2522 2976 3011 3656 3810 3835 4797 4882
Size: 51 454 35 645 154 25 962 85
Cuts at: 4882 5953 2471
Size: 1071 3254
Fragments arranged by size:
3254 1071 962 645 454 154 85 51
35 25
BsmI GAATG_Cn'
Cuts at: 4247 4340 4247
Size: 93 6643
BsmBI CGTCTCn'nnnn_
Cuts at: 2560 2560
Size: 6736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2471 2522 2823 2976 3011 3378
Size: 399 368 313 51 301 153 35 367
Cuts at: 3378 3656 3810 3835 4055 4797 4882 5953 1391
Size: 278 154 25 220 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 367 313 301
278 220 154 153 85 51 35 25
BspEI T'CCGG_A
Cuts at: 6399 6399
Size: 6736
BspHI T'CATG_A
Cuts at: 4625 6355 4625
Size: 1730 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 3359 3430 4001 4091 844
Size: 1484 1031 71 571 90 3489
Fragments arranged by size:
3489 1484 1031 571 90 71
BsrBI CCG'CTC
Cuts at: 1394 1823 2121 2602 4623 1394
Size: 429 298 481 2021 3507
Fragments arranged by size:
3507 2021 481 429 298
BsrDI GCAATG_nn'
Cuts at: 65 1248 2293 5231 5405 65
Size: 1183 1045 2938 174 1396
Fragments arranged by size:
2938 1396 1183 1045 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5380 5567 1700
Size: 110 3570 187 2869
Fragments arranged by size:
3570 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 2874 4796 5808 2874
Size: 1922 1012 3802
Fragments arranged by size:
3802 1922 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3855 794
Size: 3061 3675
BstBI TT'CG_AA
Cuts at: 3233 5540 3233
Size: 2307 4429
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6736
BstYI r'GATC_y
Cuts at: 1972 3145 3660 4066 4433 4818 4835 6287 6373
Size: 1173 515 406 367 385 17 1452 86
Cuts at: 6373 6385 6731 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1173 515 406 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2259 2259
Size: 6736
Bsu36I CC'TnA_GG
Cuts at: 2949 3021 2949
Size: 72 6664
BtgI C'CryG_G
Cuts at: 513 1065 2004 2865 513
Size: 552 939 861 4384
Fragments arranged by size:
4384 939 861 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3051 487
Size: 683 56 1825 4172
Fragments arranged by size:
4172 1825 683 56
BtsI GCAGTG_nn'
Cuts at: 3133 4252 5046 5066 3133
Size: 1119 794 20 4803
Fragments arranged by size:
4803 1119 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6701
DraI TTT'AAA
Cuts at: 2110 4040 2110
Size: 1930 4806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5743 809
Size: 507 4427 1802
Fragments arranged by size:
4427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2065 3724 4183
Size: 54 1425 50 275 18 235 1659 459
Cuts at: 4183 5072 6701 8
Size: 889 1629 43
Fragments arranged by size:
1659 1629 1425 889 459 275 235 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4183 1537
Size: 275 2371 4090
Fragments arranged by size:
4090 2371 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2527 4666 1353
Size: 1174 2139 3423
Fragments arranged by size:
3423 2139 1174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5323 5697 5843 387
Size: 1064 138 3734 374 146 1280
Fragments arranged by size:
3734 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2469 2520 3009 3654 3833 2469
Size: 51 489 645 179 5372
Fragments arranged by size:
5372 645 489 179 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2658 3344 3468 3541 3956 4799 5396
Size: 393 1026 686 124 73 415 843 597
Cuts at: 5396 6183 6451 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1026 843 787 686 597 415 393
268 124 73
EcoNI CCTnn'n_nnAGG
Cuts at: 3556 3556
Size: 6736
EcoO109I rG'GnC_Cy
Cuts at: 3334 3375 3334
Size: 41 6695
EcoRI G'AATT_C
Cuts at: 4045 4423 4045
Size: 378 6358
EcoRV GAT'ATC
Cuts at: 3178 3178
Size: 6736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3814 3814
Size: 6736
FspI TGC'GCA
Cuts at: 2531 5242 2531
Size: 2711 4025
HaeII r_GCGC'y
Cuts at: 1678 2418 2431 2617 5883 1678
Size: 740 13 186 3266 2531
Fragments arranged by size:
3266 2531 740 186 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2445 2477 3029 3061 3075
Size: 32 135 32 873 32 552 32 14
Cuts at: 3075 3107 3137 3169 3200 3232 3764 3796 3940
Size: 32 30 32 31 32 532 32 144
Cuts at: 3940 3972 4000 4032 5377 5409 5451 5483 1373
Size: 32 28 32 1345 32 42 32 2626
Fragments arranged by size:
2626 1345 873 552 532 144 135 42
32 32 32 32 32 32 32 32
32 32 32 32 31 30 28 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3756 4080 4326 669
Size: 988 281 1818 324 246 3079
Fragments arranged by size:
3079 1818 988 324 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6736
HpaI GTT'AAC
Cuts at: 4326 4326
Size: 6736
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 4335 4335
Size: 6736
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2644 5850 6034 1305
Size: 1339 3206 184 2007
Fragments arranged by size:
3206 2007 1339 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2067 3726 10
Size: 54 1425 343 235 1659 3020
Fragments arranged by size:
3020 1659 1425 343 235 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2192 2270 2981 3900 3912 4694
Size: 648 924 102 78 711 919 12 782
Cuts at: 4694 5053 5212 518
Size: 359 159 2042
Fragments arranged by size:
2042 924 919 782 711 648 359 159
102 78 12
MspA1I CmG'CkG
Cuts at: 1606 2044 2203 2449 4152 4829 5977 6222 1606
Size: 438 159 246 1703 677 1148 245 2120
Fragments arranged by size:
2120 1703 1148 677 438 246 245 159
NcoI C'CATG_G
Cuts at: 513 1065 2004 2865 513
Size: 552 939 861 4384
Fragments arranged by size:
4384 939 861 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NspI r_CATG'y
Cuts at: 1132 1229 2139 2349 2425 4094 5639 1132
Size: 97 910 210 76 1669 1545 2229
Fragments arranged by size:
2229 1669 1545 910 210 97 76
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6736
PciI A'CATG_T
Cuts at: 5635 5635
Size: 6736
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3187 3359 1108
Size: 2079 172 4485
Fragments arranged by size:
4485 2079 172
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 6318 6350 4805
Size: 32 1481 32 5191
Fragments arranged by size:
5191 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 4306 4306
Size: 6736
PspOMI G'GGCC_C
Cuts at: 3374 3374
Size: 6736
PstI C_TGCA'G
Cuts at: 830 2596 3320 3370 3427 3772 4088 830
Size: 1766 724 50 57 345 316 3478
Fragments arranged by size:
3478 1766 724 345 316 57 50
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2044 2203 2449 2044
Size: 159 246 6331
Fragments arranged by size:
6331 246 159
SacI G_AGCT'C
Cuts at: 2471 2522 3011 3656 3835 2471
Size: 51 489 645 179 5372
Fragments arranged by size:
5372 645 489 179 51
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SapI GCTCTTCn'nnn_
Cuts at: 2527 2527
Size: 6736
SbfI CC_TGCA'GG
Cuts at: 3370 4088 3370
Size: 718 6018
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SfcI C'TryA_G
Cuts at: 826 1045 2441 2592 3316 3366 3423 3768 4084
Size: 219 1396 151 724 50 57 345 316
Cuts at: 4084 5219 5900 6091 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1396 1135 724 681 345 316 219
191 151 57 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 4063 1872
Size: 2191 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2802 4840 5741 6003 820
Size: 197 926 859 2038 901 262 1553
Fragments arranged by size:
2038 1553 926 901 859 262 197
SnaBI TAC'GTA
Cuts at: 493 4527 493
Size: 4034 2702
SpeI A'CTAG_T
Cuts at: 152 2894 3308 152
Size: 2742 414 3580
Fragments arranged by size:
3580 2742 414
SphI G_CATG'C
Cuts at: 1132 2139 2425 4094 1132
Size: 1007 286 1669 3774
Fragments arranged by size:
3774 1669 1007 286
SspI AAT'ATT
Cuts at: 5 52 3295 4660 5
Size: 47 3243 1365 2081
Fragments arranged by size:
3243 2081 1365 47
StuI AGG'CCT
Cuts at: 1517 2231 3445 3797 1517
Size: 714 1214 352 4456
Fragments arranged by size:
4456 1214 714 352
StyI C'CwwG_G
Cuts at: 513 1065 2004 2865 2959 3493 3578 4175 513
Size: 552 939 861 94 534 85 597 3074
Fragments arranged by size:
3074 939 861 597 552 534 94 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4763 4780 4933 5118 5563 325
Size: 1255 3183 17 153 185 445 1498
Fragments arranged by size:
3183 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4982 6694 96
Size: 274 80 33 51 1443 3005 1712 138
Fragments arranged by size:
3005 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6696 6728 6696
Size: 32 6704
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6736
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2060 2272 3532 2060
Size: 212 1260 5264
Fragments arranged by size:
5264 1260 212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6736
XmaI C'CCGG_G
Cuts at: 1870 4061 1870
Size: 2191 4545
XmnI GAAnn'nnTTC
Cuts at: 4865 4865
Size: 6736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4544 276
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI
DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI