Restriction map for
pFN21AA0348
(Circular) (Six-base) MAPSORT of: pFN21AA0348.seq Check: 4400 from: 1 to: 9193
pFN21AA0348 9193 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2887 5182 6048 6127 6457 2887
Size: 2295 866 79 330 5623
Fragments arranged by size:
5623 2295 866 330 79
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3471 7003 278
Size: 53 83 186 936 1935 3532 2468
Fragments arranged by size:
3532 2468 1935 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3471 6536 1937
Size: 1534 3065 4594
Fragments arranged by size:
4594 3065 1534
Acc65I G'GTAC_C
Cuts at: 5998 6514 5998
Size: 516 8677
AclI AA'CG_TT
Cuts at: 1563 7320 7693 1563
Size: 5757 373 3063
Fragments arranged by size:
5757 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2570 2780 2897 3395 3821 5358 6357
Size: 677 261 210 117 498 426 1537 999
Cuts at: 6357 7256 8640 8908 1632
Size: 899 1384 268 1917
Fragments arranged by size:
1917 1537 1384 999 899 677 498 426
268 261 210 117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8996
AflIII A'CryG_T
Cuts at: 1203 2181 8092 9069 1203
Size: 978 5911 977 1327
Fragments arranged by size:
5911 1327 978 977
AgeI A'CCGG_T
Cuts at: 5348 8024 5348
Size: 2676 6517
AhdI GACnn_n'nnGTC
Cuts at: 3561 5029 6358 7922 3561
Size: 1468 1329 1564 4832
Fragments arranged by size:
4832 1564 1468 1329
AleI CACnn'nnGTG
Cuts at: 1166 2264 2664 1166
Size: 1098 400 7695
Fragments arranged by size:
7695 1098 400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2039 6135 1184
Size: 855 4096 4242
Fragments arranged by size:
4242 4096 855
AlwNI CAG_nnn'CTG
Cuts at: 2285 4041 4810 6049 6936 8508 2285
Size: 1756 769 1239 887 1572 2970
Fragments arranged by size:
2970 1756 1572 1239 887 769
ApaI G_GGCC'C
Cuts at: 4664 5805 4664
Size: 1141 8052
ApaLI G'TGCA_C
Cuts at: 7250 8406 7250
Size: 1156 8037
ApoI r'AATT_y
Cuts at: 1493 2274 3572 3580 4259 4296 5078 6502 6728
Size: 781 1298 8 679 37 782 1424 226
Cuts at: 6728 6880 8989 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1424 1298 782 781 679 226
152 37 8
AseI AT'TA_AT
Cuts at: 160 1032 7747 160
Size: 872 6715 1606
Fragments arranged by size:
6715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9193
AvaI C'yCGr_G
Cuts at: 1870 1943 2380 3209 3700 4623 5111 5847 6004
Size: 73 437 829 491 923 488 736 157
Cuts at: 6004 6518 1870
Size: 514 4545
Fragments arranged by size:
4545 923 829 736 514 491 488 437
157 73
BamHI G'GATC_C
Cuts at: 3540 3591 6523 6890 3540
Size: 51 2932 367 5843
Fragments arranged by size:
5843 2932 367 51
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 4001 4523 5396 5570
Size: 325 217 627 250 1964 522 873 174
Cuts at: 5570 5671 5977 5998 6111 6128 6191 6514 7969
Size: 101 306 21 113 17 63 323 1455
Cuts at: 7969 618
Size: 1842
Fragments arranged by size:
1964 1842 1455 873 627 522 325 323
306 250 217 174 113 101 63 21
17
BanII G_rGCy'C
Cuts at: 1391 3346 3388 4664 4749 5235 5637 5805 6305
Size: 1955 42 1276 85 486 402 168 500
Cuts at: 6305 6335 6512 1391
Size: 30 177 4072
Fragments arranged by size:
4072 1955 1276 500 486 402 177 168
85 42 30
BbeI G_GCGC'C
Cuts at: 2041 4005 5675 5981 2041
Size: 1964 1670 306 5253
Fragments arranged by size:
5253 1964 1670 306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3096 3974 4104 4210 4907 5058 5067
Size: 971 1197 878 130 106 697 151 9
Cuts at: 5067 6127 928
Size: 1060 3994
Fragments arranged by size:
3994 1197 1060 971 878 697 151 130
106 9
BbvCI CC'TCA_GC
Cuts at: 2204 2375 5451 2204
Size: 171 3076 5946
Fragments arranged by size:
5946 3076 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4601 4635 7384 7418 1903
Size: 34 2664 34 2749 34 3678
Fragments arranged by size:
3678 2749 2664 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7085 8295 7085
Size: 1210 7983
BclI T'GATC_A
Cuts at: 1548 3000 4264 4564 1548
Size: 1452 1264 300 6177
Fragments arranged by size:
6177 1452 1264 300
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2129 2515 2985
Size: 107 122 71 631 605 457 386 470
Cuts at: 2985 5528 7804 136
Size: 2543 2276 1525
Fragments arranged by size:
2543 2276 1525 631 605 470 457 386
122 107 71
BglII A'GATC_T
Cuts at: 9188 9188
Size: 9193
BlpI GC'TnA_GC
Cuts at: 4360 6610 4360
Size: 2250 6943
Bme1580I G_kGCm'C
Cuts at: 1790 2511 4528 4664 5805 5994 6114 6131 7254
Size: 721 2017 136 1141 189 120 17 1123
Cuts at: 7254 8410 1790
Size: 1156 2573
Fragments arranged by size:
2573 2017 1156 1141 1123 721 189 136
120 17
BmgBI CAC'GTC
Cuts at: 1318 2226 1318
Size: 908 8285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3094 6350 6436
Size: 416 53 83 399 24 1677 3256 86
Cuts at: 6436 6924 7882 442
Size: 488 958 1753
Fragments arranged by size:
3256 1753 1677 958 488 416 399 86
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5141 5304 5141
Size: 163 9030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2118 4476 5015 5993 6056 7853 1239
Size: 854 25 2358 539 978 63 1797 2579
Fragments arranged by size:
2579 2358 1797 978 854 539 63 25
Bpu10I CC'TnA_GC
Cuts at: 2012 2204 2375 3317 3653 5451 2012
Size: 192 171 942 336 1798 5754
Fragments arranged by size:
5754 1798 942 336 192 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2533 3082 3215 5219 5752 7318 8183 8481 2533
Size: 549 133 2004 533 1566 865 298 3245
Fragments arranged by size:
3245 2004 1566 865 549 533 298 133
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2421 3047 3303 4521 5101 5292 5326
Size: 617 922 626 256 1218 580 191 34
Cuts at: 5326 5654 7856 882
Size: 328 2202 2219
Fragments arranged by size:
2219 2202 1218 922 626 617 580 328
256 191 34
BsaAI yAC'GTr
Cuts at: 493 1204 2506 6984 493
Size: 711 1302 4478 2702
Fragments arranged by size:
4478 2702 1302 711
BsaBI GATnn'nnATC
Cuts at: 9187 9187
Size: 9193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2038 3468 3897 4002
Size: 53 83 186 936 505 1430 429 105
Cuts at: 4002 5672 5978 7000 7382 8017 275
Size: 1670 306 1022 382 635 1451
Fragments arranged by size:
1670 1451 1430 1022 936 635 505 429
382 306 186 105 83 53
BsaWI w'CCGG_w
Cuts at: 3594 3639 5348 5544 5758 7626 8024 8298 8445
Size: 45 1709 196 214 1868 398 274 147
Cuts at: 8445 8856 3594
Size: 411 3931
Fragments arranged by size:
3931 1868 1709 411 398 274 214 196
147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2116 2146 2922 2952 4501
Size: 30 165 30 713 30 776 30 1549
Cuts at: 4501 4531 5943 5973 1178
Size: 30 1412 30 4398
Fragments arranged by size:
4398 1549 1412 776 713 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 2948 3494 4946 5148 5380 5580 6022
Size: 1171 591 546 1452 202 232 200 442
Cuts at: 6022 1186
Size: 4357
Fragments arranged by size:
4357 1452 1171 591 546 442 232 202
200
BseYI C'CCAG_C
Cuts at: 2032 3693 3733 5162 8396 8892 2032
Size: 1661 40 1429 3234 496 2333
Fragments arranged by size:
3234 2333 1661 1429 496 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2242 3065 3212 1163
Size: 573 506 823 147 7144
Fragments arranged by size:
7144 823 573 506 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2086 4068 4587 6643 7404 8432
Size: 275 186 85 1982 519 2056 761 1028
Cuts at: 8432 1540
Size: 2301
Fragments arranged by size:
2301 2056 1982 1028 761 519 275 186
85
BsiHKAI G_wGCw'C
Cuts at: 2943 3654 4624 4749 5235 5449 6305 7254 7339
Size: 711 970 125 486 214 856 949 85
Cuts at: 7339 8410 2943
Size: 1071 3726
Fragments arranged by size:
3726 1071 970 949 856 711 486 214
125 85
BsiWI C'GTAC_G
Cuts at: 2184 2184
Size: 9193
BsmI GAATG_Cn'
Cuts at: 6704 6797 6704
Size: 93 9100
BsmBI CGTCTCn'nnnn_
Cuts at: 2232 2626 5112 2232
Size: 394 2486 6313
Fragments arranged by size:
6313 2486 394
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2511 2943 3346 3388 3654 4528 4624
Size: 399 721 432 403 42 266 874 96
Cuts at: 4624 4664 4749 5235 5449 5637 5805 5994 6114
Size: 40 85 486 214 188 168 189 120
Cuts at: 6114 6131 6305 6335 6512 7254 7339 8410 1391
Size: 17 174 30 177 742 85 1071 2174
Fragments arranged by size:
2174 1071 874 742 721 486 432 403
399 266 214 189 188 177 174 168
120 96 85 85 42 40 30 17
BspEI T'CCGG_A
Cuts at: 3594 3639 8856 3594
Size: 45 5217 3931
Fragments arranged by size:
5217 3931 45
BspHI T'CATG_A
Cuts at: 4156 6403 7082 8812 4156
Size: 2247 679 1730 4537
Fragments arranged by size:
4537 2247 1730 679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2887 4743 5182 5274 6034 6048 6127 6457
Size: 2043 1856 439 92 760 14 79 330
Cuts at: 6457 6548 844
Size: 91 3489
Fragments arranged by size:
3489 2043 1856 760 439 330 92 91
79 14
BsrBI CCG'CTC
Cuts at: 1394 1823 4090 7080 1394
Size: 429 2267 2990 3507
Fragments arranged by size:
3507 2990 2267 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7688 7862 65
Size: 1183 6440 174 1396
Fragments arranged by size:
6440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 5304 5348 7837 8024 1700
Size: 110 301 3193 44 2489 187 2869
Fragments arranged by size:
3193 2869 2489 301 187 110 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9193
BssHII G'CGCG_C
Cuts at: 1925 2078 1925
Size: 153 9040
BssSI C'ACGA_G
Cuts at: 7253 8265 7253
Size: 1012 8181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2869 6049 794
Size: 2075 3180 3938
Fragments arranged by size:
3938 3180 2075
BstBI TT'CG_AA
Cuts at: 7997 7997
Size: 9193
BstEII G'GTnAC_C
Cuts at: 1184 2569 1184
Size: 1385 7808
BstXI CCAn_nnnn'nTGG
Cuts at: 2666 2701 5152 2666
Size: 35 2451 6707
Fragments arranged by size:
6707 2451 35
BstYI r'GATC_y
Cuts at: 1972 3540 3591 6343 6523 6890 7275 7292 8744
Size: 1568 51 2752 180 367 385 17 1452
Cuts at: 8744 8830 8842 9188 1972
Size: 86 12 346 1977
Fragments arranged by size:
2752 1977 1568 1452 385 367 346 180
86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 6418 6418
Size: 9193
BtgI C'CryG_G
Cuts at: 513 1065 2004 2665 5401 5595 6123 513
Size: 552 939 661 2736 194 528 3583
Fragments arranged by size:
3583 2736 939 661 552 528 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3187 6201 6215 487
Size: 683 56 1961 3014 14 3465
Fragments arranged by size:
3465 3014 1961 683 56 14
BtsI GCAGTG_nn'
Cuts at: 2301 4890 4974 6100 6461 6709 7503 7523 2301
Size: 2589 84 1126 361 248 794 20 3971
Fragments arranged by size:
3971 2589 1126 794 361 248 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9158
DraI TTT'AAA
Cuts at: 6497 6497
Size: 9193
DraIII CAC_nnn'GTG
Cuts at: 2527 2869 6430 2527
Size: 342 3561 5290
Fragments arranged by size:
5290 3561 342
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2432 4499 8200 809
Size: 507 1116 2067 3701 1802
Fragments arranged by size:
3701 2067 1802 1116 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2121 2402
Size: 54 1425 50 275 18 253 38 281
Cuts at: 2402 2683 3307 4065 5312 6640 7529 9158 8
Size: 281 624 758 1247 1328 889 1629 43
Fragments arranged by size:
1629 1425 1328 1247 889 758 624 281
281 275 253 54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2083 4065 6640 1537
Size: 275 271 1982 2575 4090
Fragments arranged by size:
4090 2575 1982 275 271
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 2328 3756 4704 7123 1353
Size: 972 3 1428 948 2419 3423
Fragments arranged by size:
3423 2419 1428 972 948 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2943 3677 5255 7780 8154 8300
Size: 1064 138 1354 734 1578 2525 374 146
Cuts at: 8300 387
Size: 1280
Fragments arranged by size:
2525 1578 1354 1280 1064 734 374 146
138
EcoICRI GAG'CTC
Cuts at: 4747 5233 6303 4747
Size: 486 1070 7637
Fragments arranged by size:
7637 1070 486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2118 2309 2570 2780 2897 3395
Size: 393 461 25 191 261 210 117 498
Cuts at: 3395 3821 4476 5015 5358 5993 6056 6357 7256
Size: 426 655 539 343 635 63 301 899
Cuts at: 7256 7853 8640 8908 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 899 787 655 635 597 539 498
461 426 393 343 301 268 261 210
191 117 63 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3320 5734 5946 3320
Size: 2414 212 6567
Fragments arranged by size:
6567 2414 212
EcoO109I rG'GnC_Cy
Cuts at: 3214 3609 4660 4661 4732 5608 5802 5824 6046
Size: 395 1051 1 71 876 194 22 222
Cuts at: 6046 6076 6241 3214
Size: 30 165 6166
Fragments arranged by size:
6166 1051 876 395 222 194 165 71
30 22 1
EcoRI G'AATT_C
Cuts at: 2274 3572 5078 6502 6880 2274
Size: 1298 1506 1424 378 4587
Fragments arranged by size:
4587 1506 1424 1298 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5505 5746 5505
Size: 241 8952
FspI TGC'GCA
Cuts at: 7699 7699
Size: 9193
HaeII r_GCGC'y
Cuts at: 1678 2041 3524 4005 5675 5981 6372 8340 1678
Size: 363 1483 481 1670 306 391 1968 2531
Fragments arranged by size:
2531 1968 1670 1483 481 391 363 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2447 2479 2723 2755 3145
Size: 32 135 32 875 32 244 32 390
Cuts at: 3145 3177 3547 3551 3579 3583 4343 4375 4817
Size: 32 370 4 28 4 760 32 442
Cuts at: 4817 4849 4920 4952 5015 5047 5111 5143 5830
Size: 32 71 32 63 32 64 32 687
Cuts at: 5830 5862 6089 6121 6344 6376 7834 7866 7908
Size: 32 227 32 223 32 1458 32 42
Cuts at: 7908 7940 1373
Size: 32 2626
Fragments arranged by size:
2626 1458 875 760 687 442 390 370
244 227 223 135 71 64 63 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 28
4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3265 3876 4492 6537 6783 669
Size: 988 281 1327 611 616 2045 246 3079
Fragments arranged by size:
3079 2045 1327 988 616 611 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9193
HpaI GTT'AAC
Cuts at: 6783 6783
Size: 9193
KasI G'GCGC_C
Cuts at: 2037 4001 5671 5977 2037
Size: 1964 1670 306 5253
Fragments arranged by size:
5253 1964 1670 306
KpnI G_GTAC'C
Cuts at: 6002 6518 6002
Size: 516 8677
MfeI C'AATT_G
Cuts at: 4776 6792 4776
Size: 2016 7177
MluI A'CGCG_T
Cuts at: 2181 9069 2181
Size: 6888 2305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2718 3438 5225 5369 8307 8491 1305
Size: 1413 720 1787 144 2938 184 2007
Fragments arranged by size:
2938 2007 1787 1413 720 184 144
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 2404 3309 10
Size: 54 1425 343 291 281 905 5894
Fragments arranged by size:
5894 1425 905 343 291 281 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2664 2996 4161 7151 7510 7669
Size: 648 1098 400 332 1165 2990 359 159
Cuts at: 7669 518
Size: 2042
Fragments arranged by size:
2990 2042 1165 1098 648 400 359 332
159
MspA1I CmG'CkG
Cuts at: 1606 2497 2773 3737 3889 4454 6125 6365 6377
Size: 891 276 964 152 565 1671 240 12
Cuts at: 6377 6609 7286 8434 8679 1606
Size: 232 677 1148 245 2120
Fragments arranged by size:
2120 1671 1148 964 891 677 565 276
245 240 232 152 12
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 9193
NarI GG'CG_CC
Cuts at: 2038 4002 5672 5978 2038
Size: 1964 1670 306 5253
Fragments arranged by size:
5253 1964 1670 306
NcoI C'CATG_G
Cuts at: 513 1065 2004 5401 513
Size: 552 939 3397 4305
Fragments arranged by size:
4305 3397 939 552
NdeI CA'TA_TG
Cuts at: 387 4049 387
Size: 3662 5531
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 9193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9193
NotI GC'GGCC_GC
Cuts at: 2083 6640 2083
Size: 4557 4636
NspI r_CATG'y
Cuts at: 1132 1229 3505 6551 8096 1132
Size: 97 2276 3046 1545 2229
Fragments arranged by size:
3046 2276 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3238 1348
Size: 1890 7303
PciI A'CATG_T
Cuts at: 8092 8092
Size: 9193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2411 4911 6227 1108
Size: 1303 2500 1316 4074
Fragments arranged by size:
4074 2500 1316 1303
PfoI T'CCnGG_A
Cuts at: 4638 4848 5553 4638
Size: 210 705 8278
Fragments arranged by size:
8278 705 210
PmeI GTTT'AAAC
Cuts at: 6497 6497
Size: 9193
PmlI CAC'GTG
Cuts at: 2506 2506
Size: 9193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2751 2783 7262 7294 8775 8807 2751
Size: 32 4479 32 1481 32 3137
Fragments arranged by size:
4479 3137 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3214 3609 4732 5608 6046 6241 3214
Size: 395 1123 876 438 195 6166
Fragments arranged by size:
6166 1123 876 438 395 195
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9193
PsiI TTA'TAA
Cuts at: 5493 6763 5493
Size: 1270 7923
PspOMI G'GGCC_C
Cuts at: 4660 5801 4660
Size: 1141 8052
PspXI vC'TCGA_Gb
Cuts at: 3209 5847 3209
Size: 2638 6555
PstI C_TGCA'G
Cuts at: 830 3228 4533 5899 6045 6054 6468 6545 830
Size: 2398 1305 1366 146 9 414 77 3478
Fragments arranged by size:
3478 2398 1366 1305 414 146 77 9
PvuI CG_AT'CG
Cuts at: 2001 4587 2001
Size: 2586 6607
PvuII CAG'CTG
Cuts at: 2497 3737 3889 4454 6365 6377 2497
Size: 1240 152 565 1911 12 5313
Fragments arranged by size:
5313 1911 1240 565 152 12
SacI G_AGCT'C
Cuts at: 4749 5235 6305 4749
Size: 486 1070 7637
Fragments arranged by size:
7637 1070 486
SacII CC_GC'GG
Cuts at: 6126 6126
Size: 9193
SalI G'TCGA_C
Cuts at: 1936 6535 1936
Size: 4599 4594
SbfI CC_TGCA'GG
Cuts at: 6045 6545 6045
Size: 500 8693
ScaI AGT'ACT
Cuts at: 3805 7441 9153 3805
Size: 3636 1712 3845
Fragments arranged by size:
3845 3636 1712
SexAI A'CCwGG_T
Cuts at: 2523 4511 5223 2523
Size: 1988 712 6493
Fragments arranged by size:
6493 1988 712
SfcI C'TryA_G
Cuts at: 826 1045 2059 3224 4271 4277 4529 5895 6041
Size: 219 1014 1165 1047 6 252 1366 146
Cuts at: 6041 6050 6464 6541 7676 8357 8548 826
Size: 9 414 77 1135 681 191 1471
Fragments arranged by size:
1471 1366 1165 1135 1047 1014 681 414
252 219 191 146 77 9 6
SfoI GGC'GCC
Cuts at: 2039 4003 5673 5979 2039
Size: 1964 1670 306 5253
Fragments arranged by size:
5253 1964 1670 306
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9193
SmaI CCC'GGG
Cuts at: 1872 6520 1872
Size: 4648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2512 3061 3209 3230 5234 5731
Size: 197 926 569 549 148 21 2004 497
Cuts at: 5731 5847 7297 8198 8460 820
Size: 116 1450 901 262 1553
Fragments arranged by size:
2004 1553 1450 926 901 569 549 497
262 197 148 116 21
SnaBI TAC'GTA
Cuts at: 493 6984 493
Size: 6491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9193
SphI G_CATG'C
Cuts at: 1132 3505 6551 1132
Size: 2373 3046 3774
Fragments arranged by size:
3774 3046 2373
SspI AAT'ATT
Cuts at: 5 52 3790 7117 5
Size: 47 3738 3327 2081
Fragments arranged by size:
3738 3327 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 2832 1517
Size: 1095 220 7878
Fragments arranged by size:
7878 1095 220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2607 3360 4758 4798 4905
Size: 552 939 333 270 753 1398 40 107
Cuts at: 4905 5401 5463 5603 5994 6381 6632 513
Size: 496 62 140 391 387 251 3074
Fragments arranged by size:
3074 1398 939 753 552 496 391 387
333 270 251 140 107 62 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3192 4130 4607 4912 5284 5414 5716
Size: 1255 1612 938 477 305 372 130 302
Cuts at: 5716 6129 6418 7220 7237 7390 7575 8020 325
Size: 413 289 802 17 153 185 445 1498
Fragments arranged by size:
1612 1498 1255 938 802 477 445 413
372 305 302 289 185 153 130 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 7439 9151
Size: 274 80 33 51 1443 1826 3636 1712
Cuts at: 9151 96
Size: 138
Fragments arranged by size:
3636 1826 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2116 2148 2922 2954 4501 4533 5943 5975 9153
Size: 32 774 32 1547 32 1410 32 3178
Cuts at: 9153 9185 2116
Size: 32 2124
Fragments arranged by size:
3178 2124 1547 1410 774 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9193
XbaI T'CTAG_A
Cuts at: 4471 4483 6529 4471
Size: 12 2046 7135
Fragments arranged by size:
7135 2046 12
XhoI C'TCGA_G
Cuts at: 1943 3209 5847 1943
Size: 1266 2638 5289
Fragments arranged by size:
5289 2638 1266
XmaI C'CCGG_G
Cuts at: 1870 6518 1870
Size: 4648 4545
XmnI GAAnn'nnTTC
Cuts at: 2480 3834 7322 2480
Size: 1354 3488 4351
Fragments arranged by size:
4351 3488 1354
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3469 7001 276
Size: 53 83 186 936 1935 3532 2468
Fragments arranged by size:
3532 2468 1935 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PsrI RsrII SanDI
SapI SfiI SrfI SwaI XcmI