Restriction map for
pFN21AA0347
(Circular) (Six-base) MAPSORT of: pFN21AA0347.seq Check: 4881 from: 1 to: 8470
pFN21AA0347 8470 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2434 3746 2434
Size: 1312 7158
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6280 278
Size: 53 83 186 936 4744 2468
Fragments arranged by size:
4744 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5813 1937
Size: 3876 4594
Acc65I G'GTAC_C
Cuts at: 5791 5791
Size: 8470
AclI AA'CG_TT
Cuts at: 1563 6597 6970 1563
Size: 5034 373 3063
Fragments arranged by size:
5034 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 2653 3833 5531 6533 7917 8185 1632
Size: 746 275 1180 1698 1002 1384 268 1917
Fragments arranged by size:
1917 1698 1384 1180 1002 746 275 268
AfeI AGC'GCT
Cuts at: 3849 3849
Size: 8470
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8273
AflIII A'CryG_T
Cuts at: 1203 3330 4509 7369 8346 1203
Size: 2127 1179 2860 977 1327
Fragments arranged by size:
2860 2127 1327 1179 977
AgeI A'CCGG_T
Cuts at: 7301 7301
Size: 8470
AhdI GACnn_n'nnGTC
Cuts at: 7199 7199
Size: 8470
AleI CACnn'nnGTG
Cuts at: 1166 2499 3120 1166
Size: 1333 621 6516
Fragments arranged by size:
6516 1333 621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4686 1184
Size: 3502 4968
AlwNI CAG_nnn'CTG
Cuts at: 2175 2476 3085 4595 4640 5054 5563 6213 7785
Size: 301 609 1510 45 414 509 650 1572
Cuts at: 7785 2175
Size: 2860
Fragments arranged by size:
2860 1572 1510 650 609 509 414 301
45
ApaI G_GGCC'C
Cuts at: 3317 4124 4452 3317
Size: 807 328 7335
Fragments arranged by size:
7335 807 328
ApaLI G'TGCA_C
Cuts at: 2954 6527 7683 2954
Size: 3573 1156 3741
Fragments arranged by size:
3741 3573 1156
ApoI r'AATT_y
Cuts at: 1493 2021 2979 2997 3549 5473 5779 6005 6157
Size: 528 958 18 552 1924 306 226 152
Cuts at: 6157 8266 1493
Size: 2109 1697
Fragments arranged by size:
2109 1924 1697 958 552 528 306 226
152 18
AseI AT'TA_AT
Cuts at: 160 1032 7024 160
Size: 872 5992 1606
Fragments arranged by size:
5992 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8470
AvaI C'yCGr_G
Cuts at: 1870 1943 3707 4416 4747 4899 5085 5209 5266
Size: 73 1764 709 331 152 186 124 57
Cuts at: 5266 5795 1870
Size: 529 4545
Fragments arranged by size:
4545 1764 709 529 331 186 152 124
73 57
BamHI G'GATC_C
Cuts at: 5404 5735 5800 6167 5404
Size: 331 65 367 7707
Fragments arranged by size:
7707 367 331 65
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2698 4612 4705 5104 5791
Size: 325 217 627 911 1914 93 399 687
Cuts at: 5791 7246 618
Size: 1455 1842
Fragments arranged by size:
1914 1842 1455 911 687 627 399 325
217 93
BanII G_rGCy'C
Cuts at: 1391 2058 2067 3317 3390 4124 4161 4452 5216
Size: 667 9 1250 73 734 37 291 764
Cuts at: 5216 5240 5789 1391
Size: 24 549 4072
Fragments arranged by size:
4072 1250 764 734 667 549 291 73
37 24 9
BbeI G_GCGC'C
Cuts at: 2702 4709 5108 2702
Size: 2007 399 6064
Fragments arranged by size:
6064 2007 399
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 5730 928
Size: 971 402 3429 3668
Fragments arranged by size:
3668 3429 971 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2564 2598 4012 4046 6661 6695 1903
Size: 34 627 34 1414 34 2615 34 3678
Fragments arranged by size:
3678 2615 1414 627 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2006 6362 7572 2006
Size: 4356 1210 2904
Fragments arranged by size:
4356 2904 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8470
BfrBI ATG'CAT
Cuts at: 2796 2796
Size: 8470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4119 4703 4901
Size: 107 122 71 631 605 2447 584 198
Cuts at: 4901 7081 136
Size: 2180 1525
Fragments arranged by size:
2447 2180 1525 631 605 584 198 122
107 71
BglII A'GATC_T
Cuts at: 8465 8465
Size: 8470
BlpI GC'TnA_GC
Cuts at: 5203 5887 5203
Size: 684 7786
Bme1580I G_kGCm'C
Cuts at: 1790 2517 2958 3317 4124 4452 4539 4650 6531
Size: 727 441 359 807 328 87 111 1881
Cuts at: 6531 7687 1790
Size: 1156 2573
Fragments arranged by size:
2573 1881 1156 807 727 441 359 328
111 87
BmgBI CAC'GTC
Cuts at: 1318 2512 3331 4512 5160 5617 1318
Size: 1194 819 1181 648 457 4171
Fragments arranged by size:
4171 1194 1181 819 648 457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2031 3092 4510
Size: 416 53 83 399 24 614 1061 1418
Cuts at: 4510 4544 4725 5258 6201 7159 442
Size: 34 181 533 943 958 1753
Fragments arranged by size:
1753 1418 1061 958 943 614 533 416
399 181 83 53 34 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8470
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3934 4846 3934
Size: 912 7558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 3424 3487 3624 3904 3921 4643 5012
Size: 862 1323 63 137 280 17 722 369
Cuts at: 5012 5037 5513 7130 1239
Size: 25 476 1617 2579
Fragments arranged by size:
2579 1617 1323 862 722 476 369 280
137 63 25 17
Bpu10I CC'TnA_GC
Cuts at: 3851 3851
Size: 8470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2772 6595 7460 7758 2772
Size: 3823 865 298 3484
Fragments arranged by size:
3823 3484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 4145 7133 882
Size: 617 624 2022 2988 2219
Fragments arranged by size:
2988 2219 2022 624 617
BsaAI yAC'GTr
Cuts at: 493 1204 6261 493
Size: 711 5057 2702
Fragments arranged by size:
5057 2702 711
BsaBI GATnn'nnATC
Cuts at: 8464 8464
Size: 8470
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2699 2879 3995 4706
Size: 53 83 186 936 1166 180 1116 711
Cuts at: 4706 5105 5148 5259 6277 6659 7294 275
Size: 399 43 111 1018 382 635 1451
Fragments arranged by size:
1451 1166 1116 1018 936 711 635 399
382 186 180 111 83 53 43
BsaWI w'CCGG_w
Cuts at: 2218 2832 4349 4436 6903 7301 7575 7722 8133
Size: 614 1517 87 2467 398 274 147 411
Cuts at: 8133 2218
Size: 2555
Fragments arranged by size:
2555 2467 1517 614 411 398 274 147
87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2496 2501 2526 2531 3422
Size: 30 165 30 1093 5 25 5 891
Cuts at: 3422 3452 5511 5541 1178
Size: 30 2059 30 4107
Fragments arranged by size:
4107 2059 1093 891 165 30 30 30
30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2546 2816 3371 3490 3553 3725 4229 5317
Size: 1360 270 555 119 63 172 504 1088
Cuts at: 5317 1186
Size: 4339
Fragments arranged by size:
4339 1360 1088 555 504 270 172 119
63
BseYI C'CCAG_C
Cuts at: 2030 2067 2072 2197 3374 3649 3832 3844 4171
Size: 37 5 125 1177 275 183 12 327
Cuts at: 4171 4528 4781 4853 7673 8169 2030
Size: 357 253 72 2820 496 2331
Fragments arranged by size:
2820 2331 1177 496 357 327 275 253
183 125 72 37 12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2785 3128 3334 3353 4033 4182 1163
Size: 573 1049 343 206 19 680 149 5451
Fragments arranged by size:
5451 1049 680 573 343 206 149 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5920 6681 7709 1540
Size: 275 186 3919 761 1028 2301
Fragments arranged by size:
3919 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 2958 3105 3390 3483 4436 6531 6616 7687
Size: 399 147 285 93 953 2095 85 1071
Cuts at: 7687 2559
Size: 3342
Fragments arranged by size:
3342 2095 1071 953 399 285 147 93
85
BsmI GAATG_Cn'
Cuts at: 2575 2798 3378 4718 5981 6074 2575
Size: 223 580 1340 1263 93 4971
Fragments arranged by size:
4971 1340 1263 580 223 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2067 2517 2559 2958 3105 3317
Size: 399 268 9 450 42 399 147 212
Cuts at: 3317 3390 3483 4124 4161 4436 4452 4539 4650
Size: 73 93 641 37 275 16 87 111
Cuts at: 4650 5216 5240 5789 6531 6616 7687 1391
Size: 566 24 549 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 641 566 549 450 399
399 275 268 212 147 111 93 87
85 73 42 37 24 16 9
BspEI T'CCGG_A
Cuts at: 2832 4349 8133 2832
Size: 1517 3784 3169
Fragments arranged by size:
3784 3169 1517
BspHI T'CATG_A
Cuts at: 3595 5445 6359 8089 3595
Size: 1850 914 1730 3976
Fragments arranged by size:
3976 1850 1730 914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 3746 4770 4989 5825 844
Size: 1590 1312 1024 219 836 3489
Fragments arranged by size:
3489 1590 1312 1024 836 219
BsrBI CCG'CTC
Cuts at: 1394 1823 4942 6357 1394
Size: 429 3119 1415 3507
Fragments arranged by size:
3507 3119 1415 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3802 6965 7139 65
Size: 1183 2554 3163 174 1396
Fragments arranged by size:
3163 2554 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3405 7114 7301 1700
Size: 110 1595 3709 187 2869
Fragments arranged by size:
3709 2869 1595 187 110
BsrGI T'GTAC_A
Cuts at: 96 2619 96
Size: 2523 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8470
BssSI C'ACGA_G
Cuts at: 2843 3104 3476 6530 7542 2843
Size: 261 372 3054 1012 3771
Fragments arranged by size:
3771 3054 1012 372 261
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5563 794
Size: 4769 3701
BstBI TT'CG_AA
Cuts at: 2312 7274 2312
Size: 4962 3508
BstEII G'GTnAC_C
Cuts at: 1184 2492 4497 1184
Size: 1308 2005 5157
Fragments arranged by size:
5157 2005 1308
BstXI CCAn_nnnn'nTGG
Cuts at: 2407 2686 5617 2407
Size: 279 2931 5260
Fragments arranged by size:
5260 2931 279
BstYI r'GATC_y
Cuts at: 1972 2613 3156 3399 4298 4439 5404 5735 5800
Size: 641 543 243 899 141 965 331 65
Cuts at: 5800 6167 6552 6569 8021 8107 8119 8465 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 965 899 641 543 385 367
346 331 243 141 86 65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2429 3078 5004 2429
Size: 649 1926 5895
Fragments arranged by size:
5895 1926 649
BtgI C'CryG_G
Cuts at: 513 1065 2058 2148 2526 3263 3675 4650 4920
Size: 552 993 90 378 737 412 975 270
Cuts at: 4920 5022 513
Size: 102 3961
Fragments arranged by size:
3961 993 975 737 552 412 378 270
102 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2688 487
Size: 683 56 1462 6269
Fragments arranged by size:
6269 1462 683 56
BtsI GCAGTG_nn'
Cuts at: 2084 2116 2176 2773 3968 4273 4568 5986 6780
Size: 32 60 597 1195 305 295 1418 794
Cuts at: 6780 6800 2084
Size: 20 3754
Fragments arranged by size:
3754 1418 1195 794 597 305 295 60
32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8435
DraI TTT'AAA
Cuts at: 5774 5774
Size: 8470
DraIII CAC_nnn'GTG
Cuts at: 2563 2710 2563
Size: 147 8323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5441 7477 809
Size: 507 4125 2036 1802
Fragments arranged by size:
4125 2036 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2409 2589 3141
Size: 54 1425 50 275 18 579 180 552
Cuts at: 3141 3517 4917 5917 6806 8435 8
Size: 376 1400 1000 889 1629 43
Fragments arranged by size:
1629 1425 1400 1000 889 579 552 376
275 180 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5917 1537
Size: 275 4105 4090
Fragments arranged by size:
4105 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 3809 5754 6400 1353
Size: 1101 1355 1945 646 3423
Fragments arranged by size:
3423 1945 1355 1101 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2016 4447 5078 7057 7431 7577
Size: 1064 138 427 2431 631 1979 374 146
Cuts at: 7577 387
Size: 1280
Fragments arranged by size:
2431 1979 1280 1064 631 427 374 146
138
EcoICRI GAG'CTC
Cuts at: 3388 3388
Size: 8470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2378 2653 3424 3487 3624 3833
Size: 393 469 277 275 771 63 137 209
Cuts at: 3833 3904 3921 4643 5012 5037 5513 5531 6533
Size: 71 17 722 369 25 476 18 1002
Cuts at: 6533 7130 7917 8185 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1002 787 771 722 597 476 469
393 369 277 275 268 209 137 71
63 25 18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2891 3077 4181 4766 2891
Size: 186 1104 585 6595
Fragments arranged by size:
6595 1104 585 186
EcoO109I rG'GnC_Cy
Cuts at: 2080 3082 3129 3314 3364 3471 4121 4448 4752
Size: 1002 47 185 50 107 650 327 304
Cuts at: 4752 4929 5194 2080
Size: 177 265 5356
Fragments arranged by size:
5356 1002 650 327 304 265 185 177
107 50 47
EcoRI G'AATT_C
Cuts at: 2979 5779 6157 2979
Size: 2800 378 5292
Fragments arranged by size:
5292 2800 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 4177 4362 2767
Size: 1410 185 6875
Fragments arranged by size:
6875 1410 185
FspI TGC'GCA
Cuts at: 6976 6976
Size: 8470
HaeII r_GCGC'y
Cuts at: 1678 2702 3851 4589 4709 5108 7617 1678
Size: 1024 1149 738 120 399 2509 2531
Fragments arranged by size:
2531 2509 1149 1024 738 399 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 2792 2824 3210
Size: 32 135 32 929 32 259 32 386
Cuts at: 3210 3242 5111 5143 5186 5218 7111 7143 7185
Size: 32 1869 32 43 32 1893 32 42
Cuts at: 7185 7217 1373
Size: 32 2626
Fragments arranged by size:
2626 1893 1869 929 386 259 135 43
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5814 6060 669
Size: 988 281 3876 246 3079
Fragments arranged by size:
3876 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2744 748
Size: 1996 6474
HpaI GTT'AAC
Cuts at: 6060 6060
Size: 8470
KasI G'GCGC_C
Cuts at: 2698 4705 5104 2698
Size: 2007 399 6064
Fragments arranged by size:
6064 2007 399
KpnI G_GTAC'C
Cuts at: 5795 5795
Size: 8470
MfeI C'AATT_G
Cuts at: 6069 6069
Size: 8470
MluI A'CGCG_T
Cuts at: 8346 8346
Size: 8470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3213 5691 7584 7768 1305
Size: 1908 2478 1893 184 2007
Fragments arranged by size:
2478 2007 1908 1893 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 3143 10
Size: 54 1425 343 579 732 5337
Fragments arranged by size:
5337 1425 732 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2499 2847 3120 5363 5615 6428 6787
Size: 648 1333 348 273 2243 252 813 359
Cuts at: 6787 6946 518
Size: 159 2042
Fragments arranged by size:
2243 2042 1333 813 648 359 348 273
252 159
MspA1I CmG'CkG
Cuts at: 1606 2473 3243 3435 3653 3759 4471 4524 4990
Size: 867 770 192 218 106 712 53 466
Cuts at: 4990 5054 5461 5886 6563 7711 7956 1606
Size: 64 407 425 677 1148 245 2120
Fragments arranged by size:
2120 1148 867 770 712 677 466 425
407 245 218 192 106 64 53
NarI GG'CG_CC
Cuts at: 2699 4706 5105 2699
Size: 2007 399 6064
Fragments arranged by size:
6064 2007 399
NcoI C'CATG_G
Cuts at: 513 1065 3263 3675 4920 5022 513
Size: 552 2198 412 1245 102 3961
Fragments arranged by size:
3961 2198 1245 552 412 102
NdeI CA'TA_TG
Cuts at: 387 5671 387
Size: 5284 3186
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8470
NotI GC'GGCC_GC
Cuts at: 5917 5917
Size: 8470
NsiI A_TGCA'T
Cuts at: 2798 2798
Size: 8470
NspI r_CATG'y
Cuts at: 1132 1229 2764 5828 7373 1132
Size: 97 1535 3064 1545 2229
Fragments arranged by size:
3064 2229 1545 1535 97
PasI CC'CwG_GG
Cuts at: 1348 3887 1348
Size: 2539 5931
PciI A'CATG_T
Cuts at: 7369 7369
Size: 8470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3040 3899 4494 1108
Size: 1932 859 595 5084
Fragments arranged by size:
5084 1932 859 595
PfoI T'CCnGG_A
Cuts at: 3033 3033
Size: 8470
PmeI GTTT'AAAC
Cuts at: 5774 5774
Size: 8470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6539 6571 8052 8084 6539
Size: 32 1481 32 6925
Fragments arranged by size:
6925 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 3082 3471 4752 5194 2080
Size: 1002 389 1281 442 5356
Fragments arranged by size:
5356 1281 1002 442 389
PshAI GACnn'nnGTC
Cuts at: 1613 5292 1613
Size: 3679 4791
PsiI TTA'TAA
Cuts at: 6040 6040
Size: 8470
PspOMI G'GGCC_C
Cuts at: 3313 4120 4448 3313
Size: 807 328 7335
Fragments arranged by size:
7335 807 328
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 8438
PstI C_TGCA'G
Cuts at: 830 2091 2297 2377 3165 3420 4233 4974 4989
Size: 1261 206 80 788 255 813 741 15
Cuts at: 4989 5403 5822 830
Size: 414 419 3478
Fragments arranged by size:
3478 1261 813 788 741 419 414 255
206 80 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8470
PvuII CAG'CTG
Cuts at: 2473 3243 3653 3759 4524 4990 5054 5461 2473
Size: 770 410 106 765 466 64 407 5482
Fragments arranged by size:
5482 770 765 466 410 407 106 64
SacI G_AGCT'C
Cuts at: 3390 3390
Size: 8470
SalI G'TCGA_C
Cuts at: 1936 5812 1936
Size: 3876 4594
SanDI GG'GwC_CC
Cuts at: 3471 4752 3471
Size: 1281 7189
SapI GCTCTTCn'nnn_
Cuts at: 5754 5754
Size: 8470
SbfI CC_TGCA'GG
Cuts at: 5403 5822 5403
Size: 419 8051
ScaI AGT'ACT
Cuts at: 6718 8430 6718
Size: 1712 6758
SexAI A'CCwGG_T
Cuts at: 2631 2886 2631
Size: 255 8215
SfcI C'TryA_G
Cuts at: 826 1045 2087 2293 2373 3161 3416 4018 4229
Size: 219 1042 206 80 788 255 602 211
Cuts at: 4229 4970 4985 5055 5399 5519 5818 6953 7634
Size: 741 15 70 344 120 299 1135 681
Cuts at: 7634 7825 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1042 788 741 681 602 344
299 255 219 211 206 191 120 80
70 15
SfoI GGC'GCC
Cuts at: 2700 4707 5106 2700
Size: 2007 399 6064
Fragments arranged by size:
6064 2007 399
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8470
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8470
SmaI CCC'GGG
Cuts at: 1872 4901 5797 1872
Size: 3029 896 4545
Fragments arranged by size:
4545 3029 896
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2787 4416 6574 7475 7737 820
Size: 197 926 844 1629 2158 901 262 1553
Fragments arranged by size:
2158 1629 1553 926 901 844 262 197
SnaBI TAC'GTA
Cuts at: 493 6261 493
Size: 5768 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8470
SphI G_CATG'C
Cuts at: 1132 5828 1132
Size: 4696 3774
SspI AAT'ATT
Cuts at: 5 52 5309 5659 6394 5
Size: 47 5257 350 735 2081
Fragments arranged by size:
5257 2081 735 350 47
StuI AGG'CCT
Cuts at: 1517 4772 1517
Size: 3255 5215
StyI C'CwwG_G
Cuts at: 513 1065 2049 2388 3263 3675 4182 4302 4364
Size: 552 984 339 875 412 507 120 62
Cuts at: 4364 4920 5022 5909 513
Size: 556 102 887 3074
Fragments arranged by size:
3074 984 887 875 556 552 507 412
339 120 102 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3026 6497 6514 6667 6852 7297 325
Size: 1255 1446 3471 17 153 185 445 1498
Fragments arranged by size:
3471 1498 1446 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 3222 6716
Size: 274 80 33 51 1443 642 603 3494
Cuts at: 6716 8428 96
Size: 1712 138
Fragments arranged by size:
3494 1712 1443 642 603 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 3422 3454 8430 8462 2496
Size: 32 894 32 4976 32 2504
Fragments arranged by size:
4976 2504 894 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3770 1316
Size: 2454 6016
XbaI T'CTAG_A
Cuts at: 5806 5806
Size: 8470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2056 4036 2056
Size: 1980 6490
XhoI C'TCGA_G
Cuts at: 1943 4416 1943
Size: 2473 5997
XmaI C'CCGG_G
Cuts at: 1870 4899 5795 1870
Size: 3029 896 4545
Fragments arranged by size:
4545 3029 896
XmnI GAAnn'nnTTC
Cuts at: 2810 4345 4357 5682 6599 2810
Size: 1535 12 1325 917 4681
Fragments arranged by size:
4681 1535 1325 917 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6278 276
Size: 53 83 186 936 4744 2468
Fragments arranged by size:
4744 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspXI RsrII SacII SfiI SrfI SwaI