Restriction map for
pFN21AA0343
(Circular) (Six-base) MAPSORT of: pFN21AA0343.seq Check: 6492 from: 1 to: 8245
pFN21AA0343 8245 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3331 5104 3331
Size: 1773 6472
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 6055 278
Size: 53 83 186 936 3103 1416 2468
Fragments arranged by size:
3103 2468 1416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5588 1937
Size: 3651 4594
Acc65I G'GTAC_C
Cuts at: 5566 5566
Size: 8245
AclI AA'CG_TT
Cuts at: 1563 6372 6745 1563
Size: 4809 373 3063
Fragments arranged by size:
4809 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3713 4062 4271 5340 5508 6308 7692 7960
Size: 2081 349 209 1069 168 800 1384 268
Cuts at: 7960 1632
Size: 1917
Fragments arranged by size:
2081 1917 1384 1069 800 349 268 209
168
AfeI AGC'GCT
Cuts at: 2036 3832 2036
Size: 1796 6449
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8048
AflIII A'CryG_T
Cuts at: 1203 3518 3789 3791 7144 8121 1203
Size: 2315 271 2 3353 977 1327
Fragments arranged by size:
3353 2315 1327 977 271 2
AgeI A'CCGG_T
Cuts at: 7076 7076
Size: 8245
AhdI GACnn_n'nnGTC
Cuts at: 6974 6974
Size: 8245
AleI CACnn'nnGTG
Cuts at: 1166 2440 2818 3794 4202 1166
Size: 1274 378 976 408 5209
Fragments arranged by size:
5209 1274 976 408 378
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8245
AlwNI CAG_nnn'CTG
Cuts at: 2951 4226 4417 5988 7560 2951
Size: 1275 191 1571 1572 3636
Fragments arranged by size:
3636 1572 1571 1275 191
ApaI G_GGCC'C
Cuts at: 4760 4760
Size: 8245
ApaLI G'TGCA_C
Cuts at: 2088 4538 6302 7458 2088
Size: 2450 1764 1156 2875
Fragments arranged by size:
2875 2450 1764 1156
ApoI r'AATT_y
Cuts at: 1493 3480 3983 4962 4996 5002 5533 5554 5780
Size: 1987 503 979 34 6 531 21 226
Cuts at: 5780 5932 8041 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1987 1697 979 531 503 226 152
34 21 6
AseI AT'TA_AT
Cuts at: 160 1032 6799 160
Size: 872 5767 1606
Fragments arranged by size:
5767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8245
AvaI C'yCGr_G
Cuts at: 1870 1943 2184 4439 5570 1870
Size: 73 241 2255 1131 4545
Fragments arranged by size:
4545 2255 1131 241 73
BamHI G'GATC_C
Cuts at: 4864 5575 5942 4864
Size: 711 367 7167
Fragments arranged by size:
7167 711 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4029 4305 5509 5566 7021
Size: 325 217 627 2242 276 1204 57 1455
Cuts at: 7021 618
Size: 1842
Fragments arranged by size:
2242 1842 1455 1204 627 325 276 217
57
BanII G_rGCy'C
Cuts at: 1391 4707 4760 5564 1391
Size: 3316 53 804 4072
Fragments arranged by size:
4072 3316 804 53
BbeI G_GCGC'C
Cuts at: 4309 4309
Size: 8245
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 2652 4479 4613 5325 928
Size: 971 231 522 1827 134 712 3848
Fragments arranged by size:
3848 1827 971 712 522 231 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6436 6470 1903
Size: 34 50 34 4415 34 3678
Fragments arranged by size:
4415 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4366 6137 7347 4366
Size: 1771 1210 5264
Fragments arranged by size:
5264 1771 1210
BclI T'GATC_A
Cuts at: 1548 3661 4401 1548
Size: 2113 740 5392
Fragments arranged by size:
5392 2113 740
BfrBI ATG'CAT
Cuts at: 2981 2981
Size: 8245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6856 136
Size: 107 122 71 631 605 5184 1525
Fragments arranged by size:
5184 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8240 8240
Size: 8245
BlpI GC'TnA_GC
Cuts at: 3300 3839 5662 3300
Size: 539 1823 5883
Fragments arranged by size:
5883 1823 539
Bme1580I G_kGCm'C
Cuts at: 1790 2058 2092 4542 4760 6306 7462 1790
Size: 268 34 2450 218 1546 1156 2573
Fragments arranged by size:
2573 2450 1546 1156 268 218 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2866 3708 4223
Size: 416 53 83 399 24 1449 842 515
Cuts at: 4223 4544 4552 4797 5976 6934 442
Size: 321 8 245 1179 958 1753
Fragments arranged by size:
1753 1449 1179 958 842 515 416 399
321 245 83 53 24 8
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2604 4493 5458 2604
Size: 1889 965 5391
Fragments arranged by size:
5391 1889 965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2748 2837 3849 4488 4773 6905 1239
Size: 1509 89 1012 639 285 2132 2579
Fragments arranged by size:
2579 2132 1509 1012 639 285 89
Bpu10I CC'TnA_GC
Cuts at: 4528 4528
Size: 8245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2574 6370 7235 7533 2574
Size: 3796 865 298 3286
Fragments arranged by size:
3796 3286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2342 3393 6908 882
Size: 617 843 1051 3515 2219
Fragments arranged by size:
3515 2219 1051 843 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 3792 4090 4245 6036 493
Size: 711 2315 273 298 155 1791 2702
Fragments arranged by size:
2702 2315 1791 711 298 273 155
BsaBI GATnn'nnATC
Cuts at: 8239 8239
Size: 8245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4151 4306 4636 6052
Size: 53 83 186 936 2618 155 330 1416
Cuts at: 6052 6434 7069 275
Size: 382 635 1451
Fragments arranged by size:
2618 1451 1416 936 635 382 330 186
155 83 53
BsaWI w'CCGG_w
Cuts at: 6678 7076 7350 7497 7908 6678
Size: 398 274 147 411 7015
Fragments arranged by size:
7015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2385 2415 3806 3836 3847
Size: 30 165 30 982 30 1391 30 11
Cuts at: 3847 3853 3877 3883 5400 5430 1178
Size: 6 24 6 1517 30 3993
Fragments arranged by size:
3993 1517 1391 982 165 30 30 30
30 30 24 11 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3381 1186
Size: 2195 6050
BseYI C'CCAG_C
Cuts at: 3170 4068 4142 4759 7448 7944 3170
Size: 898 74 617 2689 496 3471
Fragments arranged by size:
3471 2689 898 617 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3249 1163
Size: 573 1513 6159
Fragments arranged by size:
6159 1513 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5695 6456 7484 1540
Size: 275 186 3694 761 1028 2301
Fragments arranged by size:
3694 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2092 3436 4542 4810 6306 6391 7462 2092
Size: 1344 1106 268 1496 85 1071 2875
Fragments arranged by size:
2875 1496 1344 1106 1071 268 85
BsmI GAATG_Cn'
Cuts at: 2880 3650 5756 5849 2880
Size: 770 2106 93 5276
Fragments arranged by size:
5276 2106 770 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3826 3826
Size: 8245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2092 3436 4542 4707 4760 4810
Size: 399 268 34 1344 1106 165 53 50
Cuts at: 4810 5564 6306 6391 7462 1391
Size: 754 742 85 1071 2174
Fragments arranged by size:
2174 1344 1106 1071 754 742 399 268
165 85 53 50 34
BspEI T'CCGG_A
Cuts at: 7908 7908
Size: 8245
BspHI T'CATG_A
Cuts at: 2328 3439 6134 7864 2328
Size: 1111 2695 1730 2709
Fragments arranged by size:
2709 2695 1730 1111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3331 5104 5600 844
Size: 2487 1773 496 3489
Fragments arranged by size:
3489 2487 1773 496
BsrBI CCG'CTC
Cuts at: 1394 1823 6132 1394
Size: 429 4309 3507
Fragments arranged by size:
4309 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2779 2823 3250 3345 4014 6740 6914
Size: 1183 1531 44 427 95 669 2726 174
Cuts at: 6914 65
Size: 1396
Fragments arranged by size:
2726 1531 1396 1183 669 427 174 95
44
BsrFI r'CCGG_y
Cuts at: 1700 1810 5109 5482 6889 7076 1700
Size: 110 3299 373 1407 187 2869
Fragments arranged by size:
3299 2869 1407 373 187 110
BsrGI T'GTAC_A
Cuts at: 96 2372 96
Size: 2276 5969
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8245
BssSI C'ACGA_G
Cuts at: 6305 7317 6305
Size: 1012 7233
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2978 794
Size: 2184 6061
BstBI TT'CG_AA
Cuts at: 4266 7049 4266
Size: 2783 5462
BstEII G'GTnAC_C
Cuts at: 1184 2287 1184
Size: 1103 7142
BstXI CCAn_nnnn'nTGG
Cuts at: 4026 4204 4316 4883 4026
Size: 178 112 567 7388
Fragments arranged by size:
7388 567 178 112
BstYI r'GATC_y
Cuts at: 1972 4653 4864 5575 5942 6327 6344 7796 7882
Size: 2681 211 711 367 385 17 1452 86
Cuts at: 7882 7894 8240 1972
Size: 12 346 1977
Fragments arranged by size:
2681 1977 1452 711 385 367 346 211
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7693
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3972 5114 487
Size: 683 56 2746 1142 3618
Fragments arranged by size:
3618 2746 1142 683 56
BtsI GCAGTG_nn'
Cuts at: 4122 5761 6555 6575 4122
Size: 1639 794 20 5792
Fragments arranged by size:
5792 1639 794 20
ClaI AT'CG_AT
Cuts at: 1554 2271 1554
Size: 717 7528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8210
DraI TTT'AAA
Cuts at: 3415 4992 5001 5549 3415
Size: 1577 9 548 6111
Fragments arranged by size:
6111 1577 548 9
DraIII CAC_nnn'GTG
Cuts at: 2576 2576
Size: 8245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2285 7252 809
Size: 507 969 4967 1802
Fragments arranged by size:
4967 1802 969 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3798 5422 5692
Size: 54 1425 50 275 18 1968 1624 270
Cuts at: 5692 6581 8210 8
Size: 889 1629 43
Fragments arranged by size:
1968 1629 1624 1425 889 275 270 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5692 1537
Size: 275 3880 4090
Fragments arranged by size:
4090 3880 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 3441 4127 6175 1353
Size: 719 1369 686 2048 3423
Fragments arranged by size:
3423 2048 1369 719 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2411 3814 6832 7206 7352 387
Size: 1064 138 822 1403 3018 374 146 1280
Fragments arranged by size:
3018 1403 1280 1064 822 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2748 2837 3713 3849 4062 4271 4488
Size: 393 1116 89 876 136 213 209 217
Cuts at: 4488 4773 5340 5508 6308 6905 7692 7960 1239
Size: 285 567 168 800 597 787 268 1524
Fragments arranged by size:
1524 1116 876 800 787 597 567 393
285 268 217 213 209 168 136 89
EcoNI CCTnn'n_nnAGG
Cuts at: 2304 2630 2304
Size: 326 7919
EcoO109I rG'GnC_Cy
Cuts at: 2605 3081 3783 4414 4546 4756 4943 2605
Size: 476 702 631 132 210 187 5907
Fragments arranged by size:
5907 702 631 476 210 187 132
EcoRI G'AATT_C
Cuts at: 5533 5554 5932 5533
Size: 21 378 7846
Fragments arranged by size:
7846 378 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2120 2474 2053
Size: 67 354 7824
Fragments arranged by size:
7824 354 67
FspI TGC'GCA
Cuts at: 6751 6751
Size: 8245
HaeII r_GCGC'y
Cuts at: 1678 2038 3834 4309 7392 1678
Size: 360 1796 475 3083 2531
Fragments arranged by size:
3083 2531 1796 475 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3806 3838 4816 4848 6886
Size: 32 135 32 2234 32 978 32 2038
Cuts at: 6886 6918 6960 6992 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2234 2038 978 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3736 5401 5524 5589 5835
Size: 988 281 1194 604 1665 123 65 246
Cuts at: 5835 669
Size: 3079
Fragments arranged by size:
3079 1665 1194 988 604 281 246 123
65
HindIII A'AGCT_T
Cuts at: 748 2233 748
Size: 1485 6760
HpaI GTT'AAC
Cuts at: 3132 5835 3132
Size: 2703 5542
KasI G'GCGC_C
Cuts at: 4305 4305
Size: 8245
KpnI G_GTAC'C
Cuts at: 5570 5570
Size: 8245
MfeI C'AATT_G
Cuts at: 2514 5844 2514
Size: 3330 4915
MluI A'CGCG_T
Cuts at: 8121 8121
Size: 8245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2165 3884 4859 7359 7543 1305
Size: 860 1719 975 2500 184 2007
Fragments arranged by size:
2500 2007 1719 975 860 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3800 5424 10
Size: 54 1425 343 1968 1624 2831
Fragments arranged by size:
2831 1968 1624 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2440 2818 3465 3794 4202 4881 6203
Size: 648 1274 378 647 329 408 679 1322
Cuts at: 6203 6562 6721 518
Size: 359 159 2042
Fragments arranged by size:
2042 1322 1274 679 648 647 408 378
359 329 159
MspA1I CmG'CkG
Cuts at: 1606 3780 3940 4072 4202 4448 5661 6338 7486
Size: 2174 160 132 130 246 1213 677 1148
Cuts at: 7486 7731 1606
Size: 245 2120
Fragments arranged by size:
2174 2120 1213 1148 677 246 245 160
132 130
NaeI GCC'GGC
Cuts at: 5111 5484 5111
Size: 373 7872
NarI GG'CG_CC
Cuts at: 4306 4306
Size: 8245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7693
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8245
NgoMIV G'CCGG_C
Cuts at: 5109 5482 5109
Size: 373 7872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8245
NotI GC'GGCC_GC
Cuts at: 5692 5692
Size: 8245
NsiI A_TGCA'T
Cuts at: 2983 2983
Size: 8245
NspI r_CATG'y
Cuts at: 1132 1229 4690 5603 7148 1132
Size: 97 3461 913 1545 2229
Fragments arranged by size:
3461 2229 1545 913 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8245
PciI A'CATG_T
Cuts at: 7144 7144
Size: 8245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3408 1108
Size: 2300 5945
PfoI T'CCnGG_A
Cuts at: 2240 2240
Size: 8245
PmeI GTTT'AAAC
Cuts at: 5549 5549
Size: 8245
PmlI CAC'GTG
Cuts at: 3792 3792
Size: 8245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6314 6346 7827 7859 6314
Size: 32 1481 32 6700
Fragments arranged by size:
6700 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2605 3081 3783 4546 2605
Size: 476 702 763 6304
Fragments arranged by size:
6304 763 702 476
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8245
PsiI TTA'TAA
Cuts at: 2901 5815 2901
Size: 2914 5331
PspOMI G'GGCC_C
Cuts at: 4756 4756
Size: 8245
PstI C_TGCA'G
Cuts at: 830 2401 2507 3095 5597 830
Size: 1571 106 588 2502 3478
Fragments arranged by size:
3478 2502 1571 588 106
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8245
PvuII CAG'CTG
Cuts at: 3940 4072 4202 4448 3940
Size: 132 130 246 7737
Fragments arranged by size:
7737 246 132 130
SalI G'TCGA_C
Cuts at: 1936 5587 1936
Size: 3651 4594
SanDI GG'GwC_CC
Cuts at: 4546 4546
Size: 8245
SapI GCTCTTCn'nnn_
Cuts at: 3441 4127 3441
Size: 686 7559
SbfI CC_TGCA'GG
Cuts at: 5597 5597
Size: 8245
ScaI AGT'ACT
Cuts at: 2496 6493 8205 2496
Size: 3997 1712 2536
Fragments arranged by size:
3997 2536 1712
SexAI A'CCwGG_T
Cuts at: 3339 3339
Size: 8245
SfcI C'TryA_G
Cuts at: 826 1045 2397 2503 3091 4449 5593 6728 7409
Size: 219 1352 106 588 1358 1144 1135 681
Cuts at: 7409 7600 826
Size: 191 1471
Fragments arranged by size:
1471 1358 1352 1144 1135 681 588 219
191 106
SfoI GGC'GCC
Cuts at: 4307 4307
Size: 8245
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8245
SmaI CCC'GGG
Cuts at: 1872 5572 1872
Size: 3700 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2589 6349 7250 7512 820
Size: 197 926 646 3760 901 262 1553
Fragments arranged by size:
3760 1553 926 901 646 262 197
SnaBI TAC'GTA
Cuts at: 493 6036 493
Size: 5543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8245
SphI G_CATG'C
Cuts at: 1132 5603 1132
Size: 4471 3774
SspI AAT'ATT
Cuts at: 5 52 2194 2538 6169 5
Size: 47 2142 344 3631 2081
Fragments arranged by size:
3631 2142 2081 344 47
StuI AGG'CCT
Cuts at: 1517 3358 4284 1517
Size: 1841 926 5478
Fragments arranged by size:
5478 1841 926
StyI C'CwwG_G
Cuts at: 513 1065 4666 5684 513
Size: 552 3601 1018 3074
Fragments arranged by size:
3601 3074 1018 552
SwaI ATTT'AAAT
Cuts at: 5001 5001
Size: 8245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 6272 6289 6442 6627 7072 325
Size: 1255 585 4107 17 153 185 445 1498
Fragments arranged by size:
4107 1498 1255 585 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2372 2494 3503
Size: 274 80 33 51 1443 395 122 1009
Cuts at: 3503 4290 4899 6491 8203 96
Size: 787 609 1592 1712 138
Fragments arranged by size:
1712 1592 1443 1009 787 609 395 274
138 122 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3297 3329 3656 3688 4489 4521 4713 4745 8205
Size: 32 327 32 801 32 192 32 3460
Cuts at: 8205 8237 3297
Size: 32 3305
Fragments arranged by size:
3460 3305 801 327 192 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3818 4775 1316
Size: 2502 957 4786
Fragments arranged by size:
4786 2502 957
XbaI T'CTAG_A
Cuts at: 5581 5581
Size: 8245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2238 2853 3405 3808 2238
Size: 615 552 403 6675
Fragments arranged by size:
6675 615 552 403
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8245
XmaI C'CCGG_G
Cuts at: 1870 5570 1870
Size: 3700 4545
XmnI GAAnn'nnTTC
Cuts at: 4262 5353 6374 4262
Size: 1091 1021 6133
Fragments arranged by size:
6133 1091 1021
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 6053 276
Size: 53 83 186 936 3103 1416 2468
Fragments arranged by size:
3103 2468 1416 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BsiWI BstZ17I Bsu36I
EcoICRI EcoRV FseI FspAI NruI PacI PspXI PsrI
RsrII SacI SacII SfiI SrfI