Restriction map for
pFN21AA0339
(Circular) (Six-base) MAPSORT of: pFN21AA0339.seq Check: 2085 from: 1 to: 9826
pFN21AA0339 9826 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4260 5422 4260
Size: 1162 8664
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4813 7636 278
Size: 53 83 186 936 3277 2823 2468
Fragments arranged by size:
3277 2823 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7169 1937
Size: 5232 4594
Acc65I G'GTAC_C
Cuts at: 6647 7147 6647
Size: 500 9326
AclI AA'CG_TT
Cuts at: 1563 7125 7953 8326 1563
Size: 5562 828 373 3063
Fragments arranged by size:
5562 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2128 2303 2354 2940 3512 4589 5211 5971
Size: 496 175 51 586 572 1077 622 760
Cuts at: 5971 7889 9273 9541 1632
Size: 1918 1384 268 1917
Fragments arranged by size:
1918 1917 1384 1077 760 622 586 572
496 268 175 51
AfeI AGC'GCT
Cuts at: 3215 3503 6842 3215
Size: 288 3339 6199
Fragments arranged by size:
6199 3339 288
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9629
AflIII A'CryG_T
Cuts at: 1203 2823 6540 8725 9702 1203
Size: 1620 3717 2185 977 1327
Fragments arranged by size:
3717 2185 1620 1327 977
AgeI A'CCGG_T
Cuts at: 8657 8657
Size: 9826
AhdI GACnn_n'nnGTC
Cuts at: 8555 8555
Size: 9826
AleI CACnn'nnGTG
Cuts at: 1166 2145 2702 1166
Size: 979 557 8290
Fragments arranged by size:
8290 979 557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3500 5925 1184
Size: 2316 2425 5085
Fragments arranged by size:
5085 2425 2316
AlwNI CAG_nnn'CTG
Cuts at: 2636 3005 3323 5276 6064 7569 9141 2636
Size: 369 318 1953 788 1505 1572 3321
Fragments arranged by size:
3321 1953 1572 1505 788 369 318
ApaI G_GGCC'C
Cuts at: 3351 3618 4666 5424 6462 3351
Size: 267 1048 758 1038 6715
Fragments arranged by size:
6715 1048 1038 758 267
ApaLI G'TGCA_C
Cuts at: 7883 9039 7883
Size: 1156 8670
ApoI r'AATT_y
Cuts at: 1493 4710 7135 7361 7513 9622 1493
Size: 3217 2425 226 152 2109 1697
Fragments arranged by size:
3217 2425 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8380 160
Size: 872 7348 1606
Fragments arranged by size:
7348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9826
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2439 2623 2829 3248 3690 4297
Size: 73 98 398 184 206 419 442 607
Cuts at: 4297 5604 5669 5682 6020 6301 6462 7151 1870
Size: 1307 65 13 338 281 161 689 4545
Fragments arranged by size:
4545 1307 689 607 442 419 398 338
281 206 184 161 98 73 65 13
AvrII C'CTAG_G
Cuts at: 5870 5870
Size: 9826
BamHI G'GATC_C
Cuts at: 2077 6732 7156 7523 2077
Size: 4655 424 367 4380
Fragments arranged by size:
4655 4380 424 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2444 2693 2723 3592 3658
Size: 325 217 627 657 249 30 869 66
Cuts at: 3658 4370 5284 5530 6164 6201 6647 7147 8602
Size: 712 914 246 634 37 446 500 1455
Cuts at: 8602 618
Size: 1842
Fragments arranged by size:
1842 1455 914 869 712 657 634 627
500 446 325 249 246 217 66 37
30
BanII G_rGCy'C
Cuts at: 1391 3190 3255 3351 3618 3999 4018 4278 4666
Size: 1799 65 96 267 381 19 260 388
Cuts at: 4666 5424 5547 6051 6462 7118 7145 1391
Size: 758 123 504 411 656 27 4072
Fragments arranged by size:
4072 1799 758 656 504 411 388 381
267 260 123 96 65 27 19
BbeI G_GCGC'C
Cuts at: 2448 6168 6205 2448
Size: 3720 37 6069
Fragments arranged by size:
6069 3720 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 4565 4776 5037 5835 7077 928
Size: 971 285 2381 211 261 798 1242 3677
Fragments arranged by size:
3677 2381 1242 971 798 285 261 211
BbvCI CC'TCA_GC
Cuts at: 3807 4111 4758 5230 6036 3807
Size: 304 647 472 806 7597
Fragments arranged by size:
7597 806 647 472 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6580 6614 8017 8051 1903
Size: 34 4643 34 1403 34 3678
Fragments arranged by size:
4643 3678 1403 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4981 7718 8928 4981
Size: 2737 1210 5879
Fragments arranged by size:
5879 2737 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2978 3259 3296
Size: 107 122 71 631 605 1306 281 37
Cuts at: 3296 5780 8437 136
Size: 2484 2657 1525
Fragments arranged by size:
2657 2484 1525 1306 631 605 281 122
107 71 37
BglII A'GATC_T
Cuts at: 3756 9821 3756
Size: 6065 3761
BlpI GC'TnA_GC
Cuts at: 3210 5739 5882 6481 6635 7243 3210
Size: 2529 143 599 154 608 5793
Fragments arranged by size:
5793 2529 608 599 154 143
Bme1580I G_kGCm'C
Cuts at: 1790 2586 2711 3351 3447 3618 4619 4666 5265
Size: 796 125 640 96 171 1001 47 599
Cuts at: 5265 5424 5970 6462 6548 7887 9043 1790
Size: 159 546 492 86 1339 1156 2573
Fragments arranged by size:
2573 1339 1156 1001 796 640 599 546
492 171 159 125 96 86 47
BmgBI CAC'GTC
Cuts at: 1318 2826 6541 1318
Size: 1508 3715 4603
Fragments arranged by size:
4603 3715 1508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2228 2598 4609
Size: 416 53 83 399 24 811 370 2011
Cuts at: 4609 5252 5309 5379 6406 6542 7557 8515 442
Size: 643 57 70 1027 136 1015 958 1753
Fragments arranged by size:
2011 1753 1027 1015 958 811 643 416
399 370 136 83 70 57 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3520 4939 5203 5473 5735 6148 3520
Size: 1419 264 270 262 413 7198
Fragments arranged by size:
7198 1419 413 270 264 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2737 2851 3204 3300 3762 4818 5331 5535
Size: 1498 114 353 96 462 1056 513 204
Cuts at: 5535 5724 5918 6032 6587 8486 1239
Size: 189 194 114 555 1899 2579
Fragments arranged by size:
2579 1899 1498 1056 555 513 462 353
204 194 189 114 114 96
Bpu10I CC'TnA_GC
Cuts at: 3807 4111 4758 5230 6036 3807
Size: 304 647 472 806 7597
Fragments arranged by size:
7597 806 647 472 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6677 7951 8816 9114 6677
Size: 1274 865 298 7389
Fragments arranged by size:
7389 1274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2318 3419 4422 8489 882
Size: 617 819 1101 1003 4067 2219
Fragments arranged by size:
4067 2219 1101 1003 819 617
BsaAI yAC'GTr
Cuts at: 493 1204 6342 6796 6847 7617 493
Size: 711 5138 454 51 770 2702
Fragments arranged by size:
5138 2702 770 711 454 51
BsaBI GATnn'nnATC
Cuts at: 4968 6278 6785 7004 9820 4968
Size: 1310 507 219 2816 4974
Fragments arranged by size:
4974 2816 1310 507 219
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2445 3800 3860 4810
Size: 53 83 186 936 912 1355 60 950
Cuts at: 4810 5342 6165 6202 7633 8015 8650 275
Size: 532 823 37 1431 382 635 1451
Fragments arranged by size:
1451 1431 1355 950 936 912 823 635
532 382 186 83 60 53 37
BsaWI w'CCGG_w
Cuts at: 6171 6699 8259 8657 8931 9078 9489 6171
Size: 528 1560 398 274 147 411 6508
Fragments arranged by size:
6508 1560 528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 3613 3643 6113
Size: 30 165 30 1059 30 1121 30 2470
Cuts at: 6113 6143 1178
Size: 30 4861
Fragments arranged by size:
4861 2470 1121 1059 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3019 3037 3055 3357 3551 3554 3834 3837
Size: 1833 18 18 302 194 3 280 3
Cuts at: 3837 3893 3942 4276 4279 4319 4511 4559 4912
Size: 56 49 334 3 40 192 48 353
Cuts at: 4912 4916 4952 5116 5125 5128 5131 5134 5149
Size: 4 36 164 9 3 3 3 15
Cuts at: 5149 5176 5222 5387 5687 5700 5747 6059 6095
Size: 27 46 165 300 13 47 312 36
Cuts at: 6095 6107 6134 6137 6140 7052 1186
Size: 12 27 3 3 912 3960
Fragments arranged by size:
3960 1833 912 353 334 312 302 300
280 194 192 165 164 56 49 48
47 46 40 36 36 27 27 18
18 15 13 12 9 4 3 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2505 2686 3103 3254 3350 3388 3415 3453 3637
Size: 181 417 151 96 38 27 38 184
Cuts at: 3637 5417 5584 5995 9029 9525 2505
Size: 1780 167 411 3034 496 2806
Fragments arranged by size:
3034 2806 1780 496 417 411 184 181
167 151 96 38 38 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2649 3604 6869 6934 1163
Size: 573 913 955 3265 65 4055
Fragments arranged by size:
4055 3265 955 913 573 65
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2646 3772 7276 8037 9065 1540
Size: 275 186 645 1126 3504 761 1028 2301
Fragments arranged by size:
3504 2301 1126 1028 761 645 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 3999 4389 5904 6471 7887 7972 9043 2427
Size: 1572 390 1515 567 1416 85 1071 3210
Fragments arranged by size:
3210 1572 1515 1416 1071 567 390 85
BsiWI C'GTAC_G
Cuts at: 2393 6342 2393
Size: 3949 5877
BsmI GAATG_Cn'
Cuts at: 7337 7430 7337
Size: 93 9733
BsmBI CGTCTCn'nnnn_
Cuts at: 2633 2832 3738 5349 2633
Size: 199 906 1611 7110
Fragments arranged by size:
7110 1611 906 199
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 2586 2711 3190 3255 3351 3447
Size: 399 637 159 125 479 65 96 96
Cuts at: 3447 3618 3999 4018 4278 4389 4619 4666 5265
Size: 171 381 19 260 111 230 47 599
Cuts at: 5265 5424 5547 5904 5970 6051 6462 6471 6548
Size: 159 123 357 66 81 411 9 77
Cuts at: 6548 7118 7145 7887 7972 9043 1391
Size: 570 27 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 637 599 570 479 411
399 381 357 260 230 171 159 159
125 123 111 96 96 85 81 77
66 65 47 27 19 9
BspEI T'CCGG_A
Cuts at: 6171 6699 9489 6171
Size: 528 2790 6508
Fragments arranged by size:
6508 2790 528
BspHI T'CATG_A
Cuts at: 7715 9445 7715
Size: 1730 8096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3249 3901 4260 5422 5640 6339 6681 7181
Size: 2405 652 359 1162 218 699 342 500
Cuts at: 7181 844
Size: 3489
Fragments arranged by size:
3489 2405 1162 699 652 500 359 342
218
BsrBI CCG'CTC
Cuts at: 1394 1823 2661 3405 3672 4611 5212 5617 6189
Size: 429 838 744 267 939 601 405 572
Cuts at: 6189 6349 7713 1394
Size: 160 1364 3507
Fragments arranged by size:
3507 1364 939 838 744 601 572 429
405 267 160
BsrDI GCAATG_nn'
Cuts at: 65 1248 6765 8321 8495 65
Size: 1183 5517 1556 174 1396
Fragments arranged by size:
5517 1556 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3258 3279 6612 8470 8657 1700
Size: 110 1448 21 3333 1858 187 2869
Fragments arranged by size:
3333 2869 1858 1448 187 110 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9826
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9826
BssSI C'ACGA_G
Cuts at: 4592 6737 7886 8898 4592
Size: 2145 1149 1012 5520
Fragments arranged by size:
5520 2145 1149 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2730 3005 5357 6064 794
Size: 1936 275 2352 707 4556
Fragments arranged by size:
4556 2352 1936 707 275
BstBI TT'CG_AA
Cuts at: 8630 8630
Size: 9826
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9826
BstXI CCAn_nnnn'nTGG
Cuts at: 2704 4264 5527 7030 2704
Size: 1560 1263 1503 5500
Fragments arranged by size:
5500 1560 1503 1263
BstYI r'GATC_y
Cuts at: 1972 2077 2376 3756 6732 7080 7156 7523 7908
Size: 105 299 1380 2976 348 76 367 385
Cuts at: 7908 7925 9377 9463 9475 9821 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2976 1977 1452 1380 385 367 348 346
299 105 86 76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2976 4374 5367 6756 513
Size: 552 939 972 1398 993 1389 3583
Fragments arranged by size:
3583 1398 1389 993 972 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2968 6168 487
Size: 683 56 1742 3200 4145
Fragments arranged by size:
4145 3200 1742 683 56
BtsI GCAGTG_nn'
Cuts at: 2697 2994 3003 6265 6943 7342 8136 8156 2697
Size: 297 9 3262 678 399 794 20 4367
Fragments arranged by size:
4367 3262 794 678 399 297 20 9
ClaI AT'CG_AT
Cuts at: 1554 6903 1554
Size: 5349 4477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9791
DraI TTT'AAA
Cuts at: 7130 7130
Size: 9826
DraIII CAC_nnn'GTG
Cuts at: 6260 6915 6260
Size: 655 9171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 5765 6782 8833 809
Size: 507 3695 754 1017 2051 1802
Fragments arranged by size:
3695 2051 1802 1017 754 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2643 2785 2979
Size: 54 1425 50 275 18 813 142 194
Cuts at: 2979 4524 5213 5772 5916 6721 6822 6927 7273
Size: 1545 689 559 144 805 101 105 346
Cuts at: 7273 8162 9791 8
Size: 889 1629 43
Fragments arranged by size:
1629 1545 1425 889 813 805 689 559
346 275 194 144 142 105 101 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2643 7273 1537
Size: 275 831 4630 4090
Fragments arranged by size:
4630 4090 831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2432 2668 3028 3064 3540 3742 4406
Size: 1008 71 236 360 36 476 202 664
Cuts at: 4406 4836 5122 5173 5193 5199 5464 5724 5990
Size: 430 286 51 20 6 265 260 266
Cuts at: 5990 6084 6099 6111 7756 1353
Size: 94 15 12 1645 3423
Fragments arranged by size:
3423 1645 1008 664 476 430 360 286
266 265 260 236 202 94 71 51
36 20 15 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 3007 3807 6056 6204 6643
Size: 1064 138 482 936 800 2249 148 439
Cuts at: 6643 8413 8787 8933 387
Size: 1770 374 146 1280
Fragments arranged by size:
2249 1770 1280 1064 936 800 482 439
374 148 146 138
EcoICRI GAG'CTC
Cuts at: 3997 3997
Size: 9826
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2303 2354 2737 2851 2940 3204
Size: 393 496 175 51 383 114 89 264
Cuts at: 3204 3300 3512 3762 4589 4818 5211 5331 5535
Size: 96 212 250 827 229 393 120 204
Cuts at: 5535 5724 5918 5971 6032 6587 7889 8486 9273
Size: 189 194 53 61 555 1302 597 787
Cuts at: 9273 9541 1239
Size: 268 1524
Fragments arranged by size:
1524 1302 827 787 597 555 496 393
393 383 268 264 250 229 212 204
194 189 175 120 114 96 89 61
53 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3327 5723 3327
Size: 2396 7430
EcoO109I rG'GnC_Cy
Cuts at: 2038 2599 3347 3615 4131 4662 4663 5363 5421
Size: 561 748 268 516 531 1 700 58
Cuts at: 5421 5874 6007 6016 6459 2038
Size: 453 133 9 443 5405
Fragments arranged by size:
5405 748 700 561 531 516 453 443
268 133 58 9 1
EcoRI G'AATT_C
Cuts at: 7135 7513 7135
Size: 378 9448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 9826
FspI TGC'GCA
Cuts at: 2102 8332 2102
Size: 6230 3596
HaeII r_GCGC'y
Cuts at: 1678 2448 3139 3217 3505 5869 6168 6205 6613
Size: 770 691 78 288 2364 299 37 408
Cuts at: 6613 6642 6844 8973 1678
Size: 29 202 2129 2531
Fragments arranged by size:
2531 2364 2129 770 691 408 299 288
202 78 37 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2791 3790 3822 4051
Size: 32 135 32 1187 32 999 32 229
Cuts at: 4051 4083 4726 4758 4892 4924 5078 5110 5633
Size: 32 643 32 134 32 154 32 523
Cuts at: 5633 5663 5665 5695 6113 6145 6599 6631 7072
Size: 30 2 30 418 32 454 32 441
Cuts at: 7072 7104 8467 8499 8541 8573 1373
Size: 32 1363 32 42 32 2626
Fragments arranged by size:
2626 1363 1187 999 643 523 454 441
418 229 154 135 134 42 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2152 4065 7170 7416 669
Size: 988 281 214 1913 3105 246 3079
Fragments arranged by size:
3105 3079 1913 988 281 246 214
HindIII A'AGCT_T
Cuts at: 748 2746 748
Size: 1998 7828
HpaI GTT'AAC
Cuts at: 4065 7416 4065
Size: 3351 6475
KasI G'GCGC_C
Cuts at: 2444 6164 6201 2444
Size: 3720 37 6069
Fragments arranged by size:
6069 3720 37
KpnI G_GTAC'C
Cuts at: 6651 7151 6651
Size: 500 9326
MfeI C'AATT_G
Cuts at: 7425 7425
Size: 9826
MluI A'CGCG_T
Cuts at: 9702 9702
Size: 9826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3786 4995 8940 9124 1305
Size: 2481 1209 3945 184 2007
Fragments arranged by size:
3945 2481 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2787 6929 10
Size: 54 1425 343 955 4142 2907
Fragments arranged by size:
4142 2907 1425 955 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2702 3482 3972 4262 5525 5907
Size: 648 979 557 780 490 290 1263 382
Cuts at: 5907 7028 7784 8143 8302 518
Size: 1121 756 359 159 2042
Fragments arranged by size:
2042 1263 1121 979 780 756 648 557
490 382 359 290 159
MspA1I CmG'CkG
Cuts at: 1606 2678 3209 4081 4115 5999 6076 6565 6625
Size: 1072 531 872 34 1884 77 489 60
Cuts at: 6625 7242 7919 9067 9312 1606
Size: 617 677 1148 245 2120
Fragments arranged by size:
2120 1884 1148 1072 872 677 617 531
489 245 77 60 34
NaeI GCC'GGC
Cuts at: 3260 3260
Size: 9826
NarI GG'CG_CC
Cuts at: 2445 6165 6202 2445
Size: 3720 37 6069
Fragments arranged by size:
6069 3720 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 6756 513
Size: 552 939 4752 3583
Fragments arranged by size:
4752 3583 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9826
NgoMIV G'CCGG_C
Cuts at: 3258 3258
Size: 9826
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9826
NotI GC'GGCC_GC
Cuts at: 7273 7273
Size: 9826
NspI r_CATG'y
Cuts at: 1132 1229 6832 7184 8729 1132
Size: 97 5603 352 1545 2229
Fragments arranged by size:
5603 2229 1545 352 97
PasI CC'CwG_GG
Cuts at: 1348 4323 4858 1348
Size: 2975 535 6316
Fragments arranged by size:
6316 2975 535
PciI A'CATG_T
Cuts at: 8725 8725
Size: 9826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3323 5417 1108
Size: 2215 2094 5517
Fragments arranged by size:
5517 2215 2094
PfoI T'CCnGG_A
Cuts at: 4401 5895 6009 4401
Size: 1494 114 8218
Fragments arranged by size:
8218 1494 114
PmeI GTTT'AAAC
Cuts at: 7130 7130
Size: 9826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3354 3386 7895 7927 9408 9440 3354
Size: 32 4509 32 1481 32 3740
Fragments arranged by size:
4509 3740 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6007 6007
Size: 9826
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9826
PsiI TTA'TAA
Cuts at: 7396 7396
Size: 9826
PspOMI G'GGCC_C
Cuts at: 3347 3614 4662 5420 6458 3347
Size: 267 1048 758 1038 6715
Fragments arranged by size:
6715 1048 1038 758 267
PstI C_TGCA'G
Cuts at: 830 3001 5362 6069 6360 7178 830
Size: 2171 2361 707 291 818 3478
Fragments arranged by size:
3478 2361 2171 818 707 291
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9826
PvuII CAG'CTG
Cuts at: 2678 3209 4081 4115 5999 6565 2678
Size: 531 872 34 1884 566 5939
Fragments arranged by size:
5939 1884 872 566 531 34
RsrII CG'GwC_CG
Cuts at: 6615 6615
Size: 9826
SacI G_AGCT'C
Cuts at: 3999 3999
Size: 9826
SalI G'TCGA_C
Cuts at: 1936 7168 1936
Size: 5232 4594
SapI GCTCTTCn'nnn_
Cuts at: 2432 2668 3742 4406 4836 5122 2432
Size: 236 1074 664 430 286 7136
Fragments arranged by size:
7136 1074 664 430 286 236
SbfI CC_TGCA'GG
Cuts at: 5362 7178 5362
Size: 1816 8010
ScaI AGT'ACT
Cuts at: 8074 9786 8074
Size: 1712 8114
SexAI A'CCwGG_T
Cuts at: 3322 3322
Size: 9826
SfcI C'TryA_G
Cuts at: 826 1045 2997 3663 5358 6065 6356 7174 8309
Size: 219 1952 666 1695 707 291 818 1135
Cuts at: 8309 8990 9181 826
Size: 681 191 1471
Fragments arranged by size:
1952 1695 1471 1135 818 707 681 666
291 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 5780 5780
Size: 9826
SfoI GGC'GCC
Cuts at: 2446 6166 6203 2446
Size: 3720 37 6069
Fragments arranged by size:
6069 3720 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9826
SmaI CCC'GGG
Cuts at: 1872 3692 5606 6464 7153 1872
Size: 1820 1914 858 689 4545
Fragments arranged by size:
4545 1914 1820 858 689
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6692 7930 8831 9093 820
Size: 197 926 4749 1238 901 262 1553
Fragments arranged by size:
4749 1553 1238 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6342 7617 493
Size: 5849 1275 2702
Fragments arranged by size:
5849 2702 1275
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9826
SphI G_CATG'C
Cuts at: 1132 7184 1132
Size: 6052 3774
SspI AAT'ATT
Cuts at: 5 52 7750 5
Size: 47 7698 2081
Fragments arranged by size:
7698 2081 47
StuI AGG'CCT
Cuts at: 1517 2107 4145 4244 4738 5356 1517
Size: 590 2038 99 494 618 5987
Fragments arranged by size:
5987 2038 618 590 494 99
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2448 2617 2809 3565 3702
Size: 552 939 81 363 169 192 756 137
Cuts at: 3702 3990 4539 5870 6756 6969 7265 513
Size: 288 549 1331 886 213 296 3074
Fragments arranged by size:
3074 1331 939 886 756 552 549 363
296 288 213 192 169 137 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3925 4020 4336 5500 7853 7870 8023
Size: 1255 2345 95 316 1164 2353 17 153
Cuts at: 8023 8208 8653 325
Size: 185 445 1498
Fragments arranged by size:
2353 2345 1498 1255 1164 445 316 185
153 95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 8072 9784 96
Size: 274 80 33 51 1443 6095 1712 138
Fragments arranged by size:
6095 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2462 2494 3613 3645 4859 4891 9786 9818 2462
Size: 32 1119 32 1214 32 4895 32 2470
Fragments arranged by size:
4895 2470 1214 1119 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9826
XbaI T'CTAG_A
Cuts at: 7162 7162
Size: 9826
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9826
XmaI C'CCGG_G
Cuts at: 1870 3690 5604 6462 7151 1870
Size: 1820 1914 858 689 4545
Fragments arranged by size:
4545 1914 1820 858 689
XmnI GAAnn'nnTTC
Cuts at: 3369 7955 3369
Size: 4586 5240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4811 7634 276
Size: 53 83 186 936 3277 2823 2468
Fragments arranged by size:
3277 2823 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BstZ17I Bsu36I EcoRV FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI SacII
SanDI SrfI SwaI XcmI