Restriction map for
pFN21AA0338
(Circular) (Six-base) MAPSORT of: pFN21AA0338.seq Check: 5312 from: 1 to: 7348
pFN21AA0338 7348 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5158 278
Size: 53 83 186 936 3622 2468
Fragments arranged by size:
3622 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2859 4593 4691 1937
Size: 922 1734 98 4594
Fragments arranged by size:
4594 1734 922 98
Acc65I G'GTAC_C
Cuts at: 4669 4669
Size: 7348
AclI AA'CG_TT
Cuts at: 1563 5475 5848 1563
Size: 3912 373 3063
Fragments arranged by size:
3912 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 3578 3806 5411 6795 7063 1632
Size: 1907 39 228 1605 1384 268 1917
Fragments arranged by size:
1917 1907 1605 1384 268 228 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7151
AflIII A'CryG_T
Cuts at: 1203 6247 7224 1203
Size: 5044 977 1327
Fragments arranged by size:
5044 1327 977
AgeI A'CCGG_T
Cuts at: 3075 6179 3075
Size: 3104 4244
AhdI GACnn_n'nnGTC
Cuts at: 6077 6077
Size: 7348
AleI CACnn'nnGTG
Cuts at: 1166 2096 1166
Size: 930 6418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7348
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 7316
AlwNI CAG_nnn'CTG
Cuts at: 2368 3353 3407 4528 5091 6663 2368
Size: 985 54 1121 563 1572 3053
Fragments arranged by size:
3053 1572 1121 985 563 54
ApaI G_GGCC'C
Cuts at: 3730 4000 3730
Size: 270 7078
ApaLI G'TGCA_C
Cuts at: 5405 6561 5405
Size: 1156 6192
ApoI r'AATT_y
Cuts at: 1493 2506 4657 4883 5035 7144 1493
Size: 1013 2151 226 152 2109 1697
Fragments arranged by size:
2151 2109 1697 1013 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5902 160
Size: 872 4870 1606
Fragments arranged by size:
4870 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7348
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2745 2800 3723 3978 4673 1870
Size: 73 126 676 55 923 255 695 4545
Fragments arranged by size:
4545 923 695 676 255 126 73 55
BamHI G'GATC_C
Cuts at: 4678 5045 4678
Size: 367 6981
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3650 4394 4669 6124 618
Size: 325 217 627 1863 744 275 1455 1842
Fragments arranged by size:
1863 1842 1455 744 627 325 275 217
BanII G_rGCy'C
Cuts at: 1391 2654 2724 3147 3176 3546 3567 3695 3730
Size: 1263 70 423 29 370 21 128 35
Cuts at: 3730 3933 4000 4086 4667 1391
Size: 203 67 86 581 4072
Fragments arranged by size:
4072 1263 581 423 370 203 128 86
70 67 35 29 21
BbeI G_GCGC'C
Cuts at: 4398 4398
Size: 7348
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 3912 928
Size: 971 666 1347 4364
Fragments arranged by size:
4364 1347 971 666
BbvCI CC'TCA_GC
Cuts at: 2691 3408 3683 3858 2691
Size: 717 275 175 6181
Fragments arranged by size:
6181 717 275 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2060 2094 3883 3917 4035 4069 5539
Size: 34 123 34 1789 34 118 34 1470
Cuts at: 5539 5573 1903
Size: 34 3678
Fragments arranged by size:
3678 1789 1470 123 118 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5240 6450 5240
Size: 1210 6138
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7348
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5959 136
Size: 107 122 71 631 605 4287 1525
Fragments arranged by size:
4287 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2982 7343 2982
Size: 4361 2987
BlpI GC'TnA_GC
Cuts at: 2670 4765 2670
Size: 2095 5253
Bme1580I G_kGCm'C
Cuts at: 1790 3653 3730 4000 5409 6565 1790
Size: 1863 77 270 1409 1156 2573
Fragments arranged by size:
2573 1863 1409 1156 270 77
BmgBI CAC'GTC
Cuts at: 1318 3719 1318
Size: 2401 4947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2657 3607 4069
Size: 416 53 83 399 24 1240 950 462
Cuts at: 4069 4506 5079 6037 442
Size: 437 573 958 1753
Fragments arranged by size:
1753 1240 958 950 573 462 437 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7348
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 7348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2774 3805 3872 6008 1239
Size: 1535 1031 67 2136 2579
Fragments arranged by size:
2579 2136 1535 1031 67
Bpu10I CC'TnA_GC
Cuts at: 2046 2691 3141 3408 3683 3858 3927 2046
Size: 645 450 267 275 175 69 5467
Fragments arranged by size:
5467 645 450 275 267 175 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2963 4268 5473 6338 6636 2963
Size: 1305 1205 865 298 3675
Fragments arranged by size:
3675 1305 1205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 6011 882
Size: 617 2748 1764 2219
Fragments arranged by size:
2748 2219 1764 617
BsaAI yAC'GTr
Cuts at: 493 1204 5139 493
Size: 711 3935 2702
Fragments arranged by size:
3935 2702 711
BsaBI GATnn'nnATC
Cuts at: 4512 7342 4512
Size: 2830 4518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4395 5155 5537 6172
Size: 53 83 186 936 2862 760 382 635
Cuts at: 6172 275
Size: 1451
Fragments arranged by size:
2862 1451 936 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2490 3075 3183 5781 6179 6453 6600 7011 2490
Size: 585 108 2598 398 274 147 411 2827
Fragments arranged by size:
2827 2598 585 411 398 274 147 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 1178
Size: 30 165 30 915 30 6178
Fragments arranged by size:
6178 915 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2210 2708 2771 3010 3425 3613 3713
Size: 880 144 498 63 239 415 188 100
Cuts at: 3713 4634 1186
Size: 921 3900
Fragments arranged by size:
3900 921 880 498 415 239 188 144
100 63
BseYI C'CCAG_C
Cuts at: 2154 2243 2547 2674 3319 4001 4185 6551 7047
Size: 89 304 127 645 682 184 2366 496
Cuts at: 7047 2154
Size: 2455
Fragments arranged by size:
2455 2366 682 645 496 304 184 127
89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 2849 3228 1163
Size: 573 948 165 379 5283
Fragments arranged by size:
5283 948 573 379 165
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3937 4798 5559 6587 1540
Size: 275 186 1936 861 761 1028 2301
Fragments arranged by size:
2301 1936 1028 861 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 3050 3546 3821 3933 5409 5494 6565 2724
Size: 326 496 275 112 1476 85 1071 3507
Fragments arranged by size:
3507 1476 1071 496 326 275 112 85
BsmI GAATG_Cn'
Cuts at: 2836 4859 4952 2836
Size: 2023 93 5232
Fragments arranged by size:
5232 2023 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2654 2724 3050 3147 3176 3546 3567
Size: 399 864 70 326 97 29 370 21
Cuts at: 3567 3653 3695 3730 3821 3933 4000 4086 4667
Size: 86 42 35 91 112 67 86 581
Cuts at: 4667 5409 5494 6565 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 864 742 581 399 370 326
112 97 91 86 86 85 70 67
42 35 29 21
BspEI T'CCGG_A
Cuts at: 2490 7011 2490
Size: 4521 2827
BspHI T'CATG_A
Cuts at: 5237 6967 5237
Size: 1730 5618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2871 4180 4703 844
Size: 2027 1309 523 3489
Fragments arranged by size:
3489 2027 1309 523
BsrBI CCG'CTC
Cuts at: 1394 1823 5235 1394
Size: 429 3412 3507
Fragments arranged by size:
3507 3412 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5843 6017 65
Size: 1183 4595 174 1396
Fragments arranged by size:
4595 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2613 2940 3075 3237 5992 6179 1700
Size: 110 803 327 135 162 2755 187 2869
Fragments arranged by size:
2869 2755 803 327 187 162 135 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7348
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7348
BssSI C'ACGA_G
Cuts at: 5408 6420 5408
Size: 1012 6336
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7348
BstBI TT'CG_AA
Cuts at: 3903 6152 3903
Size: 2249 5099
BstEII G'GTnAC_C
Cuts at: 1184 4576 1184
Size: 3392 3956
BstYI r'GATC_y
Cuts at: 1972 2487 2973 2982 3015 3982 4678 5045 5430
Size: 515 486 9 33 967 696 367 385
Cuts at: 5430 5447 6899 6985 6997 7343 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 967 696 515 486 385 367
346 86 33 17 12 9
BstZ17I GTA'TAC
Cuts at: 4594 4594
Size: 7348
BtgI C'CryG_G
Cuts at: 513 1065 2113 3889 4248 4269 4335 513
Size: 552 1048 1776 359 21 66 3526
Fragments arranged by size:
3526 1776 1048 552 359 66 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3762 487
Size: 683 56 2536 4073
Fragments arranged by size:
4073 2536 683 56
BtsI GCAGTG_nn'
Cuts at: 4398 4864 5658 5678 4398
Size: 466 794 20 6068
Fragments arranged by size:
6068 794 466 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7348
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7313
DraI TTT'AAA
Cuts at: 4652 4652
Size: 7348
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3872 6355 809
Size: 507 2556 2483 1802
Fragments arranged by size:
2556 2483 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2353 3801
Size: 54 1425 50 275 18 286 237 1448
Cuts at: 3801 4119 4332 4536 4795 5684 7313 8
Size: 318 213 204 259 889 1629 43
Fragments arranged by size:
1629 1448 1425 889 318 286 275 259
237 213 204 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4795 1537
Size: 275 2983 4090
Fragments arranged by size:
4090 2983 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5278 1353
Size: 3925 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5935 6309 6455 387
Size: 1064 138 4346 374 146 1280
Fragments arranged by size:
4346 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 3544 3931 2722
Size: 822 387 6139
Fragments arranged by size:
6139 822 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2774 3539 3578 3805 3806 3872 5411
Size: 393 1142 765 39 227 1 66 1539
Cuts at: 5411 6008 6795 7063 1239
Size: 597 787 268 1524
Fragments arranged by size:
1539 1524 1142 787 765 597 393 268
227 66 39 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2405 2992 2405
Size: 587 6761
EcoO109I rG'GnC_Cy
Cuts at: 2021 2056 3198 3350 3552 3727 3964 3996 3997
Size: 35 1142 152 202 175 237 32 1
Cuts at: 3997 4050 4525 2021
Size: 53 475 4844
Fragments arranged by size:
4844 1142 475 237 202 175 152 53
35 32 1
EcoRI G'AATT_C
Cuts at: 4657 5035 4657
Size: 378 6970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 7348
FspI TGC'GCA
Cuts at: 5854 5854
Size: 7348
HaeII r_GCGC'y
Cuts at: 1678 3228 3900 4398 6495 1678
Size: 1550 672 498 2097 2531
Fragments arranged by size:
2531 2097 1550 672 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3368 3400 4001 4033 4244
Size: 32 135 32 1796 32 601 32 211
Cuts at: 4244 4276 5989 6021 6063 6095 1373
Size: 32 1713 32 42 32 2626
Fragments arranged by size:
2626 1796 1713 601 211 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4692 4938 669
Size: 988 281 2754 246 3079
Fragments arranged by size:
3079 2754 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2840 748
Size: 2092 5256
HpaI GTT'AAC
Cuts at: 4938 4938
Size: 7348
KasI G'GCGC_C
Cuts at: 4394 4394
Size: 7348
KpnI G_GTAC'C
Cuts at: 4673 4673
Size: 7348
MfeI C'AATT_G
Cuts at: 3051 4947 3051
Size: 1896 5452
MluI A'CGCG_T
Cuts at: 7224 7224
Size: 7348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3455 6462 6646 1305
Size: 2150 3007 184 2007
Fragments arranged by size:
3007 2150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3803 4121 4334 4538 10
Size: 54 1425 343 1971 318 213 204 2820
Fragments arranged by size:
2820 1971 1425 343 318 213 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 5306 5665 5824 518
Size: 648 930 3210 359 159 2042
Fragments arranged by size:
3210 2042 930 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2551 2590 4304 4764 5441 6589 6834
Size: 478 467 39 1714 460 677 1148 245
Cuts at: 6834 1606
Size: 2120
Fragments arranged by size:
2120 1714 1148 677 478 467 460 245
39
NaeI GCC'GGC
Cuts at: 2615 2615
Size: 7348
NarI GG'CG_CC
Cuts at: 4395 4395
Size: 7348
NcoI C'CATG_G
Cuts at: 513 1065 3889 4269 513
Size: 552 2824 380 3592
Fragments arranged by size:
3592 2824 552 380
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7348
NgoMIV G'CCGG_C
Cuts at: 2613 2613
Size: 7348
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7348
NotI GC'GGCC_GC
Cuts at: 4795 4795
Size: 7348
NspI r_CATG'y
Cuts at: 1132 1229 2642 2702 4706 6251 1132
Size: 97 1413 60 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 1413 97 60
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7348
PciI A'CATG_T
Cuts at: 6247 6247
Size: 7348
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4528 1108
Size: 3420 3928
PfoI T'CCnGG_A
Cuts at: 4289 4289
Size: 7348
PmeI GTTT'AAAC
Cuts at: 4652 4652
Size: 7348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5417 5449 6930 6962 5417
Size: 32 1481 32 5803
Fragments arranged by size:
5803 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3198 3552 3198
Size: 354 6994
PshAI GACnn'nnGTC
Cuts at: 1613 4163 1613
Size: 2550 4798
PsiI TTA'TAA
Cuts at: 4918 4918
Size: 7348
PspOMI G'GGCC_C
Cuts at: 3726 3996 3726
Size: 270 7078
PstI C_TGCA'G
Cuts at: 830 2110 2589 4158 4560 4700 830
Size: 1280 479 1569 402 140 3478
Fragments arranged by size:
3478 1569 1280 479 402 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7348
PvuII CAG'CTG
Cuts at: 2551 2590 4304 2551
Size: 39 1714 5595
Fragments arranged by size:
5595 1714 39
SacI G_AGCT'C
Cuts at: 2724 3546 3933 2724
Size: 822 387 6139
Fragments arranged by size:
6139 822 387
SalI G'TCGA_C
Cuts at: 1936 4690 1936
Size: 2754 4594
SbfI CC_TGCA'GG
Cuts at: 2110 4700 2110
Size: 2590 4758
ScaI AGT'ACT
Cuts at: 5596 7308 5596
Size: 1712 5636
SexAI A'CCwGG_T
Cuts at: 2376 2376
Size: 7348
SfcI C'TryA_G
Cuts at: 826 1045 2106 2206 2585 4154 4556 4696 5831
Size: 219 1061 100 379 1569 402 140 1135
Cuts at: 5831 6512 6703 826
Size: 681 191 1471
Fragments arranged by size:
1569 1471 1135 1061 681 402 379 219
191 140 100
SfoI GGC'GCC
Cuts at: 4396 4396
Size: 7348
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7348
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7348
SmaI CCC'GGG
Cuts at: 1872 2747 2802 3725 3980 4675 1872
Size: 875 55 923 255 695 4545
Fragments arranged by size:
4545 923 875 695 255 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2978 4283 5452 6353 6615 820
Size: 197 926 1035 1305 1169 901 262 1553
Fragments arranged by size:
1553 1305 1169 1035 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5139 493
Size: 4646 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7348
SphI G_CATG'C
Cuts at: 1132 2642 4706 1132
Size: 1510 2064 3774
Fragments arranged by size:
3774 2064 1510
SspI AAT'ATT
Cuts at: 5 52 5272 5
Size: 47 5220 2081
Fragments arranged by size:
5220 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7348
StyI C'CwwG_G
Cuts at: 513 1065 2469 2874 3153 3645 3889 4269 4787
Size: 552 1404 405 279 492 244 380 518
Cuts at: 4787 513
Size: 3074
Fragments arranged by size:
3074 1404 552 518 492 405 380 279
244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2136 2666 3166 3316 4199 4445 5375
Size: 1255 556 530 500 150 883 246 930
Cuts at: 5375 5392 5545 5730 6175 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 930 883 556 530 500 445
246 185 153 150 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5594 7306 96
Size: 274 80 33 51 1443 3617 1712 138
Fragments arranged by size:
3617 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 7308 7340 2318
Size: 32 4958 32 2326
Fragments arranged by size:
4958 2326 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2378 1316
Size: 1062 6286
XbaI T'CTAG_A
Cuts at: 4684 4684
Size: 7348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3160 4193 3160
Size: 1033 6315
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7348
XmaI C'CCGG_G
Cuts at: 1870 2745 2800 3723 3978 4673 1870
Size: 875 55 923 255 695 4545
Fragments arranged by size:
4545 923 875 695 255 55
XmnI GAAnn'nnTTC
Cuts at: 5477 5477
Size: 7348
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5156 276
Size: 53 83 186 936 3622 2468
Fragments arranged by size:
3622 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI Bsu36I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SapI
SfiI SrfI SwaI