Restriction map for
pFN21AA0333
(Circular) (Six-base) MAPSORT of: pFN21AA0333.seq Check: 9241 from: 1 to: 8272
pFN21AA0333 8272 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3865 3865
Size: 8272
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4500 6082 278
Size: 53 83 186 936 2964 1582 2468
Fragments arranged by size:
2964 2468 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5615 1937
Size: 3678 4594
Acc65I G'GTAC_C
Cuts at: 3931 5593 3931
Size: 1662 6610
AclI AA'CG_TT
Cuts at: 1563 6399 6772 1563
Size: 4836 373 3063
Fragments arranged by size:
4836 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 3813 4220 4295 4486 4749 5094 6335
Size: 848 1333 407 75 191 263 345 1241
Cuts at: 6335 7719 7987 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1333 1241 848 407 345 268
263 191 75
AfeI AGC'GCT
Cuts at: 2356 2356
Size: 8272
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8075
AflIII A'CryG_T
Cuts at: 1203 7171 8148 1203
Size: 5968 977 1327
Fragments arranged by size:
5968 1327 977
AgeI A'CCGG_T
Cuts at: 7103 7103
Size: 8272
AhdI GACnn_n'nnGTC
Cuts at: 3610 7001 3610
Size: 3391 4881
AleI CACnn'nnGTG
Cuts at: 1166 2396 4937 5283 1166
Size: 1230 2541 346 4155
Fragments arranged by size:
4155 2541 1230 346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2984 1184
Size: 1800 6472
AlwNI CAG_nnn'CTG
Cuts at: 2173 2236 4634 6015 7587 2173
Size: 63 2398 1381 1572 2858
Fragments arranged by size:
2858 2398 1572 1381 63
ApaLI G'TGCA_C
Cuts at: 3401 6329 7485 3401
Size: 2928 1156 4188
Fragments arranged by size:
4188 2928 1156
ApoI r'AATT_y
Cuts at: 1493 4003 4011 4566 5207 5581 5807 5959 8068
Size: 2510 8 555 641 374 226 152 2109
Cuts at: 8068 1493
Size: 1697
Fragments arranged by size:
2510 2109 1697 641 555 374 226 152
8
AseI AT'TA_AT
Cuts at: 160 1032 6826 160
Size: 872 5794 1606
Fragments arranged by size:
5794 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8272
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2660 2910 3203 3479 4817 5597
Size: 73 80 637 250 293 276 1338 780
Cuts at: 5597 1870
Size: 4545
Fragments arranged by size:
4545 1338 780 637 293 276 250 80
73
BamHI G'GATC_C
Cuts at: 2553 5602 5969 2553
Size: 3049 367 4856
Fragments arranged by size:
4856 3049 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2250 3044 3175 3182
Size: 325 217 627 251 212 794 131 7
Cuts at: 3182 3779 3853 3931 5026 5593 7048 618
Size: 597 74 78 1095 567 1455 1842
Fragments arranged by size:
1842 1455 1095 794 627 597 567 325
251 217 212 131 78 74 7
BanII G_rGCy'C
Cuts at: 1391 2301 2532 2612 2661 4824 5355 5591 1391
Size: 910 231 80 49 2163 531 236 4072
Fragments arranged by size:
4072 2163 910 531 236 231 80 49
BbeI G_GCGC'C
Cuts at: 2254 3179 2254
Size: 925 7347
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2946 3096 3350 3609 3708 4382 4492
Size: 971 1047 150 254 259 99 674 110
Cuts at: 4492 4653 4842 5309 5328 928
Size: 161 189 467 19 3872
Fragments arranged by size:
3872 1047 971 674 467 259 254 189
161 150 110 99 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6463 6497 1903
Size: 34 4526 34 3678
Fragments arranged by size:
4526 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 5235 6164 7374 3266
Size: 1969 929 1210 4164
Fragments arranged by size:
4164 1969 1210 929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3842 3851 6883
Size: 107 122 71 631 605 2170 9 3032
Cuts at: 6883 136
Size: 1525
Fragments arranged by size:
3032 2170 1525 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 3153 4331 8267 3153
Size: 1178 3936 3158
Fragments arranged by size:
3936 3158 1178
BlpI GC'TnA_GC
Cuts at: 5046 5495 5689 5046
Size: 449 194 7629
Fragments arranged by size:
7629 449 194
Bme1580I G_kGCm'C
Cuts at: 1790 3405 3782 5031 6333 7489 1790
Size: 1615 377 1249 1302 1156 2573
Fragments arranged by size:
2573 1615 1302 1249 1156 377
BmgBI CAC'GTC
Cuts at: 1318 2304 5470 1318
Size: 986 3166 4120
Fragments arranged by size:
4120 3166 986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 2640 3348
Size: 416 53 83 399 24 957 266 708
Cuts at: 3348 6003 6961 442
Size: 2655 958 1753
Fragments arranged by size:
2655 1753 958 957 708 416 399 266
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 3432 4267 2350
Size: 1082 835 6355
Fragments arranged by size:
6355 1082 835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2303 2778 3051 3402 3645 4430 4785
Size: 929 135 475 273 351 243 785 355
Cuts at: 4785 5112 6932 1239
Size: 327 1820 2579
Fragments arranged by size:
2579 1820 929 785 475 355 351 327
273 243 135
Bpu10I CC'TnA_GC
Cuts at: 2733 3295 3375 2733
Size: 562 80 7630
Fragments arranged by size:
7630 562 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3134 5315 6397 7262 7560 3134
Size: 2181 1082 865 298 3846
Fragments arranged by size:
3846 2181 1082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 3019 3403 3425 6935 882
Size: 617 858 662 384 22 3510 2219
Fragments arranged by size:
3510 2219 858 662 617 384 22
BsaAI yAC'GTr
Cuts at: 493 1204 3217 4311 4460 6063 493
Size: 711 2013 1094 149 1603 2702
Fragments arranged by size:
2702 2013 1603 1094 711 149
BsaBI GATnn'nnATC
Cuts at: 4812 8266 4812
Size: 3454 4818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2251 2322 2637 3176
Size: 53 83 186 936 718 71 315 539
Cuts at: 3176 3967 4497 6079 6461 7096 275
Size: 791 530 1582 382 635 1451
Fragments arranged by size:
1582 1451 936 791 718 635 539 530
382 315 186 83 71 53
BsaWI w'CCGG_w
Cuts at: 4690 6705 7103 7377 7524 7935 4690
Size: 2015 398 274 147 411 5027
Fragments arranged by size:
5027 2015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3550 3580 3643 3673 1178
Size: 30 165 30 2147 30 63 30 5777
Fragments arranged by size:
5777 2147 165 63 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2278 2600 2765 3632 5405 1186
Size: 862 230 322 165 867 1773 4053
Fragments arranged by size:
4053 1773 867 862 322 230 165
BseYI C'CCAG_C
Cuts at: 2173 2690 4684 4852 7475 7971 2173
Size: 517 1994 168 2623 496 2474
Fragments arranged by size:
2623 2474 1994 517 496 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2944 2996 3672 3972 4343 4659 1163
Size: 573 1208 52 676 300 371 316 4776
Fragments arranged by size:
4776 1208 676 573 371 316 300 52
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2774 4900 5722 6483 7511 1540
Size: 275 186 773 2126 822 761 1028 2301
Fragments arranged by size:
2301 2126 1028 822 773 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3376 3405 4481 5355 6333 6418 7489 3376
Size: 29 1076 874 978 85 1071 4159
Fragments arranged by size:
4159 1076 1071 978 874 85 29
BsmI GAATG_Cn'
Cuts at: 5783 5876 5783
Size: 93 8179
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2532 2612 2661 3376 3405 3782
Size: 399 511 231 80 49 715 29 377
Cuts at: 3782 4481 4824 5031 5355 5591 6333 6418 7489
Size: 699 343 207 324 236 742 85 1071
Cuts at: 7489 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 715 699 511 399 377
343 324 236 231 207 85 80 49
29
BspEI T'CCGG_A
Cuts at: 4690 7935 4690
Size: 3245 5027
BspHI T'CATG_A
Cuts at: 4083 6161 7891 4083
Size: 2078 1730 4464
Fragments arranged by size:
4464 2078 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3371 3865 5627 844
Size: 2527 494 1762 3489
Fragments arranged by size:
3489 2527 1762 494
BsrBI CCG'CTC
Cuts at: 1394 1823 4869 4916 6159 1394
Size: 429 3046 47 1243 3507
Fragments arranged by size:
3507 3046 1243 429 47
BsrDI GCAATG_nn'
Cuts at: 65 1248 2035 2241 6767 6941 65
Size: 1183 787 206 4526 174 1396
Fragments arranged by size:
4526 1396 1183 787 206 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3850 5553 6916 7103 1700
Size: 110 2040 1703 1363 187 2869
Fragments arranged by size:
2869 2040 1703 1363 187 110
BsrGI T'GTAC_A
Cuts at: 96 3053 96
Size: 2957 5315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8272
BssSI C'ACGA_G
Cuts at: 3701 6332 7344 3701
Size: 2631 1012 4629
Fragments arranged by size:
4629 2631 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3872 4929 794
Size: 3078 1057 4137
Fragments arranged by size:
4137 3078 1057
BstBI TT'CG_AA
Cuts at: 2092 7076 2092
Size: 4984 3288
BstEII G'GTnAC_C
Cuts at: 1184 4876 1184
Size: 3692 4580
BstYI r'GATC_y
Cuts at: 1972 2553 3153 4331 5602 5969 6354 6371 7823
Size: 581 600 1178 1271 367 385 17 1452
Cuts at: 7823 7909 7921 8267 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1271 1178 600 581 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2043 3766 2043
Size: 1723 6549
BtgI C'CryG_G
Cuts at: 513 1065 2004 2254 3633 3669 3797 4938 5431
Size: 552 939 250 1379 36 128 1141 493
Cuts at: 5431 513
Size: 3354
Fragments arranged by size:
3354 1379 1141 939 552 493 250 128
36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2487 3978 4086 487
Size: 683 56 1261 1491 108 4673
Fragments arranged by size:
4673 1491 1261 683 108 56
BtsI GCAGTG_nn'
Cuts at: 2200 3456 5788 6582 6602 2200
Size: 1256 2332 794 20 3870
Fragments arranged by size:
3870 2332 1256 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8237
DraI TTT'AAA
Cuts at: 5576 5576
Size: 8272
DraIII CAC_nnn'GTG
Cuts at: 4978 4978
Size: 8272
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7279 809
Size: 507 5963 1802
Fragments arranged by size:
5963 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4810 4897 5434
Size: 54 1425 50 275 18 2980 87 537
Cuts at: 5434 5719 6608 8237 8
Size: 285 889 1629 43
Fragments arranged by size:
2980 1629 1425 889 537 285 275 87
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4897 5719 1537
Size: 275 3085 822 4090
Fragments arranged by size:
4090 3085 822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2422 3400 4229 4469 4553 5382 6202 1353
Size: 1069 978 829 240 84 829 820 3423
Fragments arranged by size:
3423 1069 978 829 829 820 240 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4410 6859 7233 7379 387
Size: 1064 138 2821 2449 374 146 1280
Fragments arranged by size:
2821 2449 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5353 5353
Size: 8272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2303 2480 2778 3051 3402 3645
Size: 393 536 135 177 298 273 351 243
Cuts at: 3645 3813 4220 4295 4430 4486 4749 4785 5094
Size: 168 407 75 135 56 263 36 309
Cuts at: 5094 5112 6335 6932 7719 7987 1239
Size: 18 1223 597 787 268 1524
Fragments arranged by size:
1524 1223 787 597 536 407 393 351
309 298 273 268 263 243 177 168
135 135 75 56 36 18
EcoNI CCTnn'n_nnAGG
Cuts at: 5255 5255
Size: 8272
EcoO109I rG'GnC_Cy
Cuts at: 2541 2591 4204 4903 5476 5506 2541
Size: 50 1613 699 573 30 5307
Fragments arranged by size:
5307 1613 699 573 50 30
EcoRI G'AATT_C
Cuts at: 5581 5959 5581
Size: 378 7894
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5026 5026
Size: 8272
FspI TGC'GCA
Cuts at: 6778 6778
Size: 8272
HaeII r_GCGC'y
Cuts at: 1678 2254 2358 2581 3179 4487 5502 5554 7419
Size: 576 104 223 598 1308 1015 52 1865
Cuts at: 7419 1678
Size: 2531
Fragments arranged by size:
2531 1865 1308 1015 598 576 223 104
52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 2441 2473 2545
Size: 32 135 32 716 32 121 32 72
Cuts at: 2545 2577 3065 3097 3550 3582 3596 3628 4162
Size: 32 488 32 453 32 14 32 534
Cuts at: 4162 4194 4640 4672 6913 6945 6987 7019 1373
Size: 32 446 32 2241 32 42 32 2626
Fragments arranged by size:
2626 2241 716 534 488 453 446 135
121 72 42 32 32 32 32 32
32 32 32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 5616 5862 669
Size: 988 281 3678 246 3079
Fragments arranged by size:
3678 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8272
HpaI GTT'AAC
Cuts at: 5862 5862
Size: 8272
KasI G'GCGC_C
Cuts at: 2250 3175 2250
Size: 925 7347
KpnI G_GTAC'C
Cuts at: 3935 5597 3935
Size: 1662 6610
MfeI C'AATT_G
Cuts at: 5871 5871
Size: 8272
MluI A'CGCG_T
Cuts at: 8148 8148
Size: 8272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2697 4410 7386 7570 1305
Size: 1392 1713 2976 184 2007
Fragments arranged by size:
2976 2007 1713 1392 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4812 10
Size: 54 1425 343 2980 3470
Fragments arranged by size:
3470 2980 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 4770 4937 4973 5283 6230 6589
Size: 648 1230 2374 167 36 310 947 359
Cuts at: 6589 6748 518
Size: 159 2042
Fragments arranged by size:
2374 2042 1230 947 648 359 310 167
159 36
MspA1I CmG'CkG
Cuts at: 1606 2173 2256 2396 2865 3671 3833 3863 3899
Size: 567 83 140 469 806 162 30 36
Cuts at: 3899 4700 4831 5494 5688 6365 7513 7758 1606
Size: 801 131 663 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 806 801 677 663 567 469
245 194 162 140 131 83 36 30
NaeI GCC'GGC
Cuts at: 5555 5555
Size: 8272
NarI GG'CG_CC
Cuts at: 2251 3176 2251
Size: 925 7347
NcoI C'CATG_G
Cuts at: 513 1065 2004 3633 3797 513
Size: 552 939 1629 164 4988
Fragments arranged by size:
4988 1629 939 552 164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8272
NgoMIV G'CCGG_C
Cuts at: 5553 5553
Size: 8272
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8272
NotI GC'GGCC_GC
Cuts at: 5719 5719
Size: 8272
NspI r_CATG'y
Cuts at: 1132 1229 2272 4513 5194 5630 7175 1132
Size: 97 1043 2241 681 436 1545 2229
Fragments arranged by size:
2241 2229 1545 1043 681 436 97
PasI CC'CwG_GG
Cuts at: 1348 4793 1348
Size: 3445 4827
PciI A'CATG_T
Cuts at: 7171 7171
Size: 8272
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3821 4808 4978 1108
Size: 2713 987 170 4402
Fragments arranged by size:
4402 2713 987 170
PfoI T'CCnGG_A
Cuts at: 4801 4801
Size: 8272
PmeI GTTT'AAAC
Cuts at: 5576 5576
Size: 8272
PmlI CAC'GTG
Cuts at: 3217 4311 4460 3217
Size: 1094 149 7029
Fragments arranged by size:
7029 1094 149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6341 6373 7854 7886 6341
Size: 32 1481 32 6727
Fragments arranged by size:
6727 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2591 4204 4903 5476 5506 2591
Size: 1613 699 573 30 5357
Fragments arranged by size:
5357 1613 699 573 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8272
PsiI TTA'TAA
Cuts at: 5842 5842
Size: 8272
PspXI vC'TCGA_Gb
Cuts at: 2910 2910
Size: 8272
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3924 3956 3924
Size: 32 8240
PstI C_TGCA'G
Cuts at: 830 3245 3862 5624 830
Size: 2415 617 1762 3478
Fragments arranged by size:
3478 2415 1762 617
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8272
PvuII CAG'CTG
Cuts at: 2173 2396 3833 4700 2173
Size: 223 1437 867 5745
Fragments arranged by size:
5745 1437 867 223
RsrII CG'GwC_CG
Cuts at: 3848 3848
Size: 8272
SacI G_AGCT'C
Cuts at: 5355 5355
Size: 8272
SacII CC_GC'GG
Cuts at: 2257 3672 2257
Size: 1415 6857
SalI G'TCGA_C
Cuts at: 1936 5614 1936
Size: 3678 4594
SanDI GG'GwC_CC
Cuts at: 2591 4903 2591
Size: 2312 5960
SapI GCTCTTCn'nnn_
Cuts at: 4469 4469
Size: 8272
SbfI CC_TGCA'GG
Cuts at: 5624 5624
Size: 8272
ScaI AGT'ACT
Cuts at: 6520 8232 6520
Size: 1712 6560
SexAI A'CCwGG_T
Cuts at: 5542 5542
Size: 8272
SfcI C'TryA_G
Cuts at: 826 1045 3241 3858 5620 6755 7436 7627 826
Size: 219 2196 617 1762 1135 681 191 1471
Fragments arranged by size:
2196 1762 1471 1135 681 617 219 191
SfoI GGC'GCC
Cuts at: 2252 3177 2252
Size: 925 7347
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8272
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8272
SmaI CCC'GGG
Cuts at: 1872 3205 5599 1872
Size: 1333 2394 4545
Fragments arranged by size:
4545 2394 1333
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2910 3149 5330 6376 7277 7539
Size: 197 926 967 239 2181 1046 901 262
Cuts at: 7539 820
Size: 1553
Fragments arranged by size:
2181 1553 1046 967 926 901 262 239
197
SnaBI TAC'GTA
Cuts at: 493 6063 493
Size: 5570 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8272
SphI G_CATG'C
Cuts at: 1132 2272 5630 1132
Size: 1140 3358 3774
Fragments arranged by size:
3774 3358 1140
SspI AAT'ATT
Cuts at: 5 52 6196 5
Size: 47 6144 2081
Fragments arranged by size:
6144 2081 47
StuI AGG'CCT
Cuts at: 1517 4749 1517
Size: 3232 5040
StyI C'CwwG_G
Cuts at: 513 1065 2004 3094 3633 3797 3970 5711 513
Size: 552 939 1090 539 164 173 1741 3074
Fragments arranged by size:
3074 1741 1090 939 552 539 173 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2788 4985 5226 5553 6299 6316 6469
Size: 1255 1208 2197 241 327 746 17 153
Cuts at: 6469 6654 7099 325
Size: 185 445 1498
Fragments arranged by size:
2197 1498 1255 1208 746 445 327 241
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3053 3237 6518
Size: 274 80 33 51 1443 1076 184 3281
Cuts at: 6518 8230 96
Size: 1712 138
Fragments arranged by size:
3281 1712 1443 1076 274 184 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4449 4481 8232 8264 4449
Size: 32 3751 32 4457
Fragments arranged by size:
4457 3751 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8272
XbaI T'CTAG_A
Cuts at: 5608 5608
Size: 8272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 4405 2626
Size: 1779 6493
XhoI C'TCGA_G
Cuts at: 1943 2910 1943
Size: 967 7305
XmaI C'CCGG_G
Cuts at: 1870 3203 5597 1870
Size: 1333 2394 4545
Fragments arranged by size:
4545 2394 1333
XmnI GAAnn'nnTTC
Cuts at: 2877 6401 2877
Size: 3524 4748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4498 6080 276
Size: 53 83 186 936 2964 1582 2468
Fragments arranged by size:
2964 2468 1582 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoRV FseI FspAI NruI NsiI
PacI PspOMI SfiI SrfI SwaI