Restriction map for
pFN21AA0329
(Circular) (Six-base) MAPSORT of: pFN21AA0329.seq Check: 9527 from: 1 to: 8938
pFN21AA0329 8938 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4552 5853 4552
Size: 1301 7637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4256 6748 278
Size: 53 83 186 936 2720 2492 2468
Fragments arranged by size:
2720 2492 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6281 1937
Size: 4344 4594
Acc65I G'GTAC_C
Cuts at: 6259 6259
Size: 8938
AclI AA'CG_TT
Cuts at: 1563 3641 7065 7438 1563
Size: 2078 3424 373 3063
Fragments arranged by size:
3424 3063 2078 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2619 3090 5210 7001 8385 8653 1632
Size: 987 471 2120 1791 1384 268 1917
Fragments arranged by size:
2120 1917 1791 1384 987 471 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8741
AflIII A'CryG_T
Cuts at: 1203 4150 7837 8814 1203
Size: 2947 3687 977 1327
Fragments arranged by size:
3687 2947 1327 977
AgeI A'CCGG_T
Cuts at: 7769 7769
Size: 8938
AhdI GACnn_n'nnGTC
Cuts at: 3517 7667 3517
Size: 4150 4788
AleI CACnn'nnGTG
Cuts at: 1166 2246 1166
Size: 1080 7858
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2186 4529 1184
Size: 1002 2343 5593
Fragments arranged by size:
5593 2343 1002
AlwNI CAG_nnn'CTG
Cuts at: 2024 2297 3170 3322 3994 4351 4899 5360 5845
Size: 273 873 152 672 357 548 461 485
Cuts at: 5845 6089 6681 8253 2024
Size: 244 592 1572 2709
Fragments arranged by size:
2709 1572 873 672 592 548 485 461
357 273 244 152
ApaI G_GGCC'C
Cuts at: 3976 4870 4999 5983 6119 3976
Size: 894 129 984 136 6795
Fragments arranged by size:
6795 984 894 136 129
ApaLI G'TGCA_C
Cuts at: 3004 5873 6995 8151 3004
Size: 2869 1122 1156 3791
Fragments arranged by size:
3791 2869 1156 1122
ApoI r'AATT_y
Cuts at: 1493 2651 3054 3476 5291 6057 6247 6473 6625
Size: 1158 403 422 1815 766 190 226 152
Cuts at: 6625 8734 1493
Size: 2109 1697
Fragments arranged by size:
2109 1815 1697 1158 766 422 403 226
190 152
AseI AT'TA_AT
Cuts at: 160 1032 7492 160
Size: 872 6460 1606
Fragments arranged by size:
6460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8938
AvaI C'yCGr_G
Cuts at: 1870 1943 2717 4423 4973 5246 5278 6263 1870
Size: 73 774 1706 550 273 32 985 4545
Fragments arranged by size:
4545 1706 985 774 550 273 73 32
BamHI G'GATC_C
Cuts at: 2978 6268 6635 2978
Size: 3290 367 5281
Fragments arranged by size:
5281 3290 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2190 2865 4476 4520 5321
Size: 325 217 627 403 675 1611 44 801
Cuts at: 5321 6170 6259 7714 618
Size: 849 89 1455 1842
Fragments arranged by size:
1842 1611 1455 849 801 675 627 403
325 217 89 44
BanII G_rGCy'C
Cuts at: 1391 2414 2490 2724 3270 3285 3976 4035 4326
Size: 1023 76 234 546 15 691 59 291
Cuts at: 4326 4588 4870 4999 5983 6119 6257 1391
Size: 262 282 129 984 136 138 4072
Fragments arranged by size:
4072 1023 984 691 546 291 282 262
234 138 136 129 76 59 15
BbeI G_GCGC'C
Cuts at: 4524 4524
Size: 8938
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2234 3422 3894 928
Size: 971 335 1188 472 5972
Fragments arranged by size:
5972 1188 971 472 335
BbvCI CC'TCA_GC
Cuts at: 3687 3965 4082 4445 3687
Size: 278 117 363 8180
Fragments arranged by size:
8180 363 278 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2146 2180 4954 4988 7129 7163 1903
Size: 34 209 34 2774 34 2141 34 3678
Fragments arranged by size:
3678 2774 2141 209 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6830 8040 6830
Size: 1210 7728
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3280 5607 6123
Size: 107 122 71 631 605 1608 2327 516
Cuts at: 6123 7549 136
Size: 1426 1525
Fragments arranged by size:
2327 1608 1525 1426 631 605 516 122
107 71
BglII A'GATC_T
Cuts at: 8933 8933
Size: 8938
BlpI GC'TnA_GC
Cuts at: 2258 5960 6355 2258
Size: 3702 395 4841
Fragments arranged by size:
4841 3702 395
Bme1580I G_kGCm'C
Cuts at: 1790 3008 3976 4870 4999 5877 5983 6119 6999
Size: 1218 968 894 129 878 106 136 880
Cuts at: 6999 8155 1790
Size: 1156 2573
Fragments arranged by size:
2573 1218 1156 968 894 880 878 136
129 106
BmgBI CAC'GTC
Cuts at: 1318 5299 1318
Size: 3981 4957
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2655 3490 5742
Size: 416 53 83 399 24 1238 835 2252
Cuts at: 5742 6669 7627 442
Size: 927 958 1753
Fragments arranged by size:
2252 1753 1238 958 927 835 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4607
Size: 8938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2457 3122 3269 3558 3674 6236 7598 1239
Size: 1218 665 147 289 116 2562 1362 2579
Fragments arranged by size:
2579 2562 1362 1218 665 289 147 116
Bpu10I CC'TnA_GC
Cuts at: 3297 3687 3965 4082 4352 4445 4700 5304 3297
Size: 390 278 117 270 93 255 604 6931
Fragments arranged by size:
6931 604 390 278 270 255 117 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3514 4244 4370 7063 7928 8226 3514
Size: 730 126 2693 865 298 4226
Fragments arranged by size:
4226 2693 865 730 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2569 4192 4462 5991 7601 882
Size: 617 1070 1623 270 1529 1610 2219
Fragments arranged by size:
2219 1623 1610 1529 1070 617 270
BsaAI yAC'GTr
Cuts at: 493 1204 3133 5878 6729 493
Size: 711 1929 2745 851 2702
Fragments arranged by size:
2745 2702 1929 851 711
BsaBI GATnn'nnATC
Cuts at: 3079 8932 3079
Size: 5853 3085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4253 4374 4521 6018
Size: 53 83 186 936 2720 121 147 1497
Cuts at: 6018 6745 7127 7762 275
Size: 727 382 635 1451
Fragments arranged by size:
2720 1497 1451 936 727 635 382 186
147 121 83 53
BsaWI w'CCGG_w
Cuts at: 2981 4183 4903 7371 7769 8043 8190 8601 2981
Size: 1202 720 2468 398 274 147 411 3318
Fragments arranged by size:
3318 2468 1202 720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3245 3275 3427 3457 6209
Size: 30 165 30 1842 30 152 30 2752
Cuts at: 6209 6239 1178
Size: 30 3877
Fragments arranged by size:
3877 2752 1842 165 152 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2528 4280 5266 5912 6122 1186
Size: 865 477 1752 986 646 210 4002
Fragments arranged by size:
4002 1752 986 865 646 477 210
BseYI C'CCAG_C
Cuts at: 2499 2901 3301 3969 4034 4341 4360 5217 5637
Size: 402 400 668 65 307 19 857 420
Cuts at: 5637 8141 8637 2499
Size: 2504 496 2800
Fragments arranged by size:
2800 2504 857 668 496 420 402 400
307 65 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 2798 3155 3585 4386 5134 5470
Size: 573 816 246 357 430 801 748 336
Cuts at: 5470 5513 1163
Size: 43 4588
Fragments arranged by size:
4588 816 801 748 573 430 357 336
246 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6388 7149 8177 1540
Size: 275 186 4387 761 1028 2301
Fragments arranged by size:
4387 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3008 3440 4326 5877 5966 6999 7084 8155 3008
Size: 432 886 1551 89 1033 85 1071 3791
Fragments arranged by size:
3791 1551 1071 1033 886 432 89 85
BsmI GAATG_Cn'
Cuts at: 6449 6542 6449
Size: 93 8845
BsmBI CGTCTCn'nnnn_
Cuts at: 3167 4301 3167
Size: 1134 7804
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2414 2490 2724 3008 3270 3285 3440
Size: 399 624 76 234 284 262 15 155
Cuts at: 3440 3976 4035 4326 4588 4870 4999 5877 5966
Size: 536 59 291 262 282 129 878 89
Cuts at: 5966 5983 6119 6257 6999 7084 8155 1391
Size: 17 136 138 742 85 1071 2174
Fragments arranged by size:
2174 1071 878 742 624 536 399 291
284 282 262 262 234 155 138 136
129 89 85 76 59 17 15
BspEI T'CCGG_A
Cuts at: 4903 8601 4903
Size: 3698 5240
BspHI T'CATG_A
Cuts at: 6827 8557 6827
Size: 1730 7208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4552 5853 6293 844
Size: 3708 1301 440 3489
Fragments arranged by size:
3708 3489 1301 440
BsrBI CCG'CTC
Cuts at: 1394 1823 2100 6825 1394
Size: 429 277 4725 3507
Fragments arranged by size:
4725 3507 429 277
BsrDI GCAATG_nn'
Cuts at: 65 1248 2330 7433 7607 65
Size: 1183 1082 5103 174 1396
Fragments arranged by size:
5103 1396 1183 1082 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2187 4356 7582 7769 1700
Size: 110 377 2169 3226 187 2869
Fragments arranged by size:
3226 2869 2169 377 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8938
BssHII G'CGCG_C
Cuts at: 1925 5518 1925
Size: 3593 5345
BssSI C'ACGA_G
Cuts at: 6998 8010 6998
Size: 1012 7926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8938
BstBI TT'CG_AA
Cuts at: 7742 7742
Size: 8938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8938
BstXI CCAn_nnnn'nTGG
Cuts at: 3208 3734 3902 3208
Size: 526 168 8244
Fragments arranged by size:
8244 526 168
BstYI r'GATC_y
Cuts at: 1972 2082 2473 2978 5271 6268 6635 7020 7037
Size: 110 391 505 2293 997 367 385 17
Cuts at: 7037 8489 8575 8587 8933 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2293 1977 1452 997 505 391 385 367
346 110 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4579 6207 4579
Size: 1628 7310
BtgI C'CryG_G
Cuts at: 513 1065 2004 2746 3195 3207 4026 4235 4282
Size: 552 939 742 449 12 819 209 47
Cuts at: 4282 5008 5347 5487 5604 513
Size: 726 339 140 117 3847
Fragments arranged by size:
3847 939 819 742 726 552 449 339
209 140 117 47 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 4275 4719 487
Size: 683 56 905 2144 444 4706
Fragments arranged by size:
4706 2144 905 683 444 56
BtsI GCAGTG_nn'
Cuts at: 3784 4692 4922 6454 7248 7268 3784
Size: 908 230 1532 794 20 5454
Fragments arranged by size:
5454 1532 908 794 230 20
ClaI AT'CG_AT
Cuts at: 1554 2012 1554
Size: 458 8480
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8903
DraI TTT'AAA
Cuts at: 6242 6242
Size: 8938
DraIII CAC_nnn'GTG
Cuts at: 5152 5152
Size: 8938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4252 7945 809
Size: 507 2936 3693 1802
Fragments arranged by size:
3693 2936 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3198 3210 4075
Size: 54 1425 50 275 18 1368 12 865
Cuts at: 4075 5154 5566 6385 7274 8903 8
Size: 1079 412 819 889 1629 43
Fragments arranged by size:
1629 1425 1368 1079 889 865 819 412
275 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 6385 1537
Size: 275 4573 4090
Fragments arranged by size:
4573 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3196 6173 6868 1353
Size: 1843 2977 695 3423
Fragments arranged by size:
3423 2977 1843 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7525 7899 8045 387
Size: 1064 138 5936 374 146 1280
Fragments arranged by size:
5936 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4324 4324
Size: 8938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2457 2619 3090 3122 3269 3558 3674
Size: 393 825 162 471 32 147 289 116
Cuts at: 3674 5210 6236 7001 7598 8385 8653 1239
Size: 1536 1026 765 597 787 268 1524
Fragments arranged by size:
1536 1524 1026 825 787 765 597 471
393 289 268 162 147 116 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2299 4679 5567 2299
Size: 2380 888 5670
Fragments arranged by size:
5670 2380 888
EcoO109I rG'GnC_Cy
Cuts at: 3973 4348 4427 4738 4866 4867 4996 5443 6211
Size: 375 79 311 128 1 129 447 768
Cuts at: 6211 3973
Size: 6700
Fragments arranged by size:
6700 768 447 375 311 129 128 79
1
EcoRI G'AATT_C
Cuts at: 5291 6247 6625 5291
Size: 956 378 7604
Fragments arranged by size:
7604 956 378
EcoRV GAT'ATC
Cuts at: 2016 2016
Size: 8938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 4965 2233
Size: 2732 6206
FspI TGC'GCA
Cuts at: 5201 7444 5201
Size: 2243 6695
HaeII r_GCGC'y
Cuts at: 1678 4524 4853 5463 5555 5960 8085 1678
Size: 2846 329 610 92 405 2125 2531
Fragments arranged by size:
2846 2531 2125 610 405 329 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2352 2384 2504 2536 2978
Size: 32 135 32 780 32 120 32 442
Cuts at: 2978 3010 3082 3114 3245 3277 3427 3459 3503
Size: 32 72 32 131 32 150 32 44
Cuts at: 3503 3535 4753 4785 5722 5754 7579 7611 7653
Size: 32 1218 32 937 32 1825 32 42
Cuts at: 7653 7685 1373
Size: 32 2626
Fragments arranged by size:
2626 1825 1218 937 780 442 150 135
131 120 72 44 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2950 6282 6528 669
Size: 988 281 1012 3332 246 3079
Fragments arranged by size:
3332 3079 1012 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4947 5413 5662 748
Size: 4199 466 249 4024
Fragments arranged by size:
4199 4024 466 249
HpaI GTT'AAC
Cuts at: 6528 6528
Size: 8938
KasI G'GCGC_C
Cuts at: 4520 4520
Size: 8938
KpnI G_GTAC'C
Cuts at: 6263 6263
Size: 8938
MfeI C'AATT_G
Cuts at: 2961 6537 2961
Size: 3576 5362
MluI A'CGCG_T
Cuts at: 8814 8814
Size: 8938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2099 2490 2894 3919 6160 8052 8236 1305
Size: 794 391 404 1025 2241 1892 184 2007
Fragments arranged by size:
2241 2007 1892 1025 794 404 391 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3200 3212 4077 5156 10
Size: 54 1425 343 1368 12 865 1079 3792
Fragments arranged by size:
3792 1425 1368 1079 865 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2168 2246 3732 5609 6896 7255
Size: 648 984 18 78 1486 1877 1287 359
Cuts at: 7255 7414 518
Size: 159 2042
Fragments arranged by size:
2042 1877 1486 1287 984 648 359 159
78 18
MspA1I CmG'CkG
Cuts at: 1606 2503 2536 3258 3969 4177 4813 5262 5497
Size: 897 33 722 711 208 636 449 235
Cuts at: 5497 5953 5985 6354 7031 8179 8424 1606
Size: 456 32 369 677 1148 245 2120
Fragments arranged by size:
2120 1148 897 722 711 677 636 456
449 369 245 235 208 33 32
NaeI GCC'GGC
Cuts at: 2189 4358 2189
Size: 2169 6769
NarI GG'CG_CC
Cuts at: 4521 4521
Size: 8938
NcoI C'CATG_G
Cuts at: 513 1065 2004 3195 5008 513
Size: 552 939 1191 1813 4443
Fragments arranged by size:
4443 1813 1191 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8938
NgoMIV G'CCGG_C
Cuts at: 2187 4356 2187
Size: 2169 6769
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8938
NotI GC'GGCC_GC
Cuts at: 6385 6385
Size: 8938
NspI r_CATG'y
Cuts at: 1132 1229 2173 4103 5614 5673 5930 6296 7841
Size: 97 944 1930 1511 59 257 366 1545
Cuts at: 7841 1132
Size: 2229
Fragments arranged by size:
2229 1930 1545 1511 944 366 257 97
59
PasI CC'CwG_GG
Cuts at: 1348 3278 1348
Size: 1930 7008
PciI A'CATG_T
Cuts at: 7837 7837
Size: 8938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4564 1108
Size: 3456 5482
PfoI T'CCnGG_A
Cuts at: 3651 3651
Size: 8938
PmeI GTTT'AAAC
Cuts at: 6242 6242
Size: 8938
PmlI CAC'GTG
Cuts at: 3133 5878 3133
Size: 2745 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7007 7039 8520 8552 7007
Size: 32 1481 32 7393
Fragments arranged by size:
7393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4348 4427 4738 5443 6211 4348
Size: 79 311 705 768 7075
Fragments arranged by size:
7075 768 705 311 79
PshAI GACnn'nnGTC
Cuts at: 1613 4122 4184 1613
Size: 2509 62 6367
Fragments arranged by size:
6367 2509 62
PsiI TTA'TAA
Cuts at: 3050 6508 3050
Size: 3458 5480
PspOMI G'GGCC_C
Cuts at: 3972 4866 4995 5979 6115 3972
Size: 894 129 984 136 6795
Fragments arranged by size:
6795 984 894 136 129
PstI C_TGCA'G
Cuts at: 830 3817 4508 5395 6290 830
Size: 2987 691 887 895 3478
Fragments arranged by size:
3478 2987 895 887 691
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8938
PvuII CAG'CTG
Cuts at: 2503 2536 3969 4177 5497 5953 2503
Size: 33 1433 208 1320 456 5488
Fragments arranged by size:
5488 1433 1320 456 208 33
RsrII CG'GwC_CG
Cuts at: 5883 5883
Size: 8938
SacI G_AGCT'C
Cuts at: 4326 4326
Size: 8938
SalI G'TCGA_C
Cuts at: 1936 6280 1936
Size: 4344 4594
SanDI GG'GwC_CC
Cuts at: 4427 4427
Size: 8938
SapI GCTCTTCn'nnn_
Cuts at: 3196 6173 3196
Size: 2977 5961
SbfI CC_TGCA'GG
Cuts at: 6290 6290
Size: 8938
ScaI AGT'ACT
Cuts at: 2644 7186 8898 2644
Size: 4542 1712 2684
Fragments arranged by size:
4542 2684 1712
SexAI A'CCwGG_T
Cuts at: 2274 2274
Size: 8938
SfcI C'TryA_G
Cuts at: 826 1045 3813 4504 5391 6286 7421 8102 8293
Size: 219 2768 691 887 895 1135 681 191
Cuts at: 8293 826
Size: 1471
Fragments arranged by size:
2768 1471 1135 895 887 691 681 219
191
SfoI GGC'GCC
Cuts at: 4522 4522
Size: 8938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8938
SmaI CCC'GGG
Cuts at: 1872 2719 6265 1872
Size: 847 3546 4545
Fragments arranged by size:
4545 3546 847
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4223 4385 7042 7943 8205
Size: 197 926 1586 694 162 2657 901 262
Cuts at: 8205 820
Size: 1553
Fragments arranged by size:
2657 1586 1553 926 901 694 262 197
162
SnaBI TAC'GTA
Cuts at: 493 6729 493
Size: 6236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8938
SphI G_CATG'C
Cuts at: 1132 2173 4103 5614 6296 1132
Size: 1041 1930 1511 682 3774
Fragments arranged by size:
3774 1930 1511 1041 682
SrfI GCCC'GGGC
Cuts at: 2719 2719
Size: 8938
SspI AAT'ATT
Cuts at: 5 52 3021 6862 5
Size: 47 2969 3841 2081
Fragments arranged by size:
3841 2969 2081 47
StuI AGG'CCT
Cuts at: 1517 3067 4070 5945 1517
Size: 1550 1003 1875 4510
Fragments arranged by size:
4510 1875 1550 1003
StyI C'CwwG_G
Cuts at: 513 1065 2004 2633 3195 3221 4194 5008 5053
Size: 552 939 629 562 26 973 814 45
Cuts at: 5053 6377 513
Size: 1324 3074
Fragments arranged by size:
3074 1324 973 939 814 629 562 552
45 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2893 4352 5839 6188 6965 6982 7135
Size: 1255 1313 1459 1487 349 777 17 153
Cuts at: 7135 7320 7765 325
Size: 185 445 1498
Fragments arranged by size:
1498 1487 1459 1313 1255 777 445 349
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 2181 2211
Size: 274 80 33 51 1443 75 129 30
Cuts at: 2211 2642 7184 8896 96
Size: 431 4542 1712 138
Fragments arranged by size:
4542 1712 1443 431 274 138 129 80
75 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2641 2673 5531 5563 6209 6241 8898 8930 2641
Size: 32 2858 32 646 32 2657 32 2649
Fragments arranged by size:
2858 2657 2649 646 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2945 4931 6091 1316
Size: 1629 1986 1160 4163
Fragments arranged by size:
4163 1986 1629 1160
XbaI T'CTAG_A
Cuts at: 2476 6274 2476
Size: 3798 5140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 3298 5149 2533
Size: 765 1851 6322
Fragments arranged by size:
6322 1851 765
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8938
XmaI C'CCGG_G
Cuts at: 1870 2717 6263 1870
Size: 847 3546 4545
Fragments arranged by size:
4545 3546 847
XmnI GAAnn'nnTTC
Cuts at: 2093 2429 5291 7067 2093
Size: 336 2862 1776 3964
Fragments arranged by size:
3964 2862 1776 336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4254 6746 276
Size: 53 83 186 936 2720 2492 2468
Fragments arranged by size:
2720 2492 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
FseI FspAI NruI NsiI PacI PspXI PsrI SacII
SfiI SwaI