Restriction map for
pFN21AA0327
(Circular) (Six-base) MAPSORT of: pFN21AA0327.seq Check: 1522 from: 1 to: 7165
pFN21AA0327 7165 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4975 278
Size: 53 83 186 936 3439 2468
Fragments arranged by size:
3439 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4050 4508 1937
Size: 2113 458 4594
Fragments arranged by size:
4594 2113 458
Acc65I G'GTAC_C
Cuts at: 4486 4486
Size: 7165
AclI AA'CG_TT
Cuts at: 1563 5292 5665 1563
Size: 3729 373 3063
Fragments arranged by size:
3729 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4058 5228 6612 6880 1632
Size: 2426 1170 1384 268 1917
Fragments arranged by size:
2426 1917 1384 1170 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6968
AflIII A'CryG_T
Cuts at: 1203 3161 3639 6064 7041 1203
Size: 1958 478 2425 977 1327
Fragments arranged by size:
2425 1958 1327 977 478
AgeI A'CCGG_T
Cuts at: 3536 5996 3536
Size: 2460 4705
AhdI GACnn_n'nnGTC
Cuts at: 5894 5894
Size: 7165
AleI CACnn'nnGTG
Cuts at: 1166 3725 1166
Size: 2559 4606
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2244 2801 1184
Size: 1060 557 5548
Fragments arranged by size:
5548 1060 557
AlwNI CAG_nnn'CTG
Cuts at: 2474 3595 4097 4908 6480 2474
Size: 1121 502 811 1572 3159
Fragments arranged by size:
3159 1572 1121 811 502
ApaLI G'TGCA_C
Cuts at: 5222 6378 5222
Size: 1156 6009
ApoI r'AATT_y
Cuts at: 1493 2399 2843 3079 4474 4700 4852 6961 1493
Size: 906 444 236 1395 226 152 2109 1697
Fragments arranged by size:
2109 1697 1395 906 444 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2856 5719 160
Size: 872 1824 2863 1606
Fragments arranged by size:
2863 1824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7165
AvaI C'yCGr_G
Cuts at: 1870 1943 3089 3683 3796 4490 1870
Size: 73 1146 594 113 694 4545
Fragments arranged by size:
4545 1146 694 594 113 73
BamHI G'GATC_C
Cuts at: 4495 4862 4495
Size: 367 6798
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3499 3736 4486 5941 618
Size: 325 217 627 1712 237 750 1455 1842
Fragments arranged by size:
1842 1712 1455 750 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2289 2303 3898 4146 4484 1391
Size: 898 14 1595 248 338 4072
Fragments arranged by size:
4072 1595 898 338 248 14
BbeI G_GCGC'C
Cuts at: 3503 3740 3503
Size: 237 6928
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4105 928
Size: 971 2206 3988
Fragments arranged by size:
3988 2206 971
BbvCI CC'TCA_GC
Cuts at: 3629 4151 3629
Size: 522 6643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5356 5390 1903
Size: 34 3419 34 3678
Fragments arranged by size:
3678 3419 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2698 5057 6267 2698
Size: 2359 1210 3596
Fragments arranged by size:
3596 2359 1210
BclI T'GATC_A
Cuts at: 1548 2256 3045 4417 1548
Size: 708 789 1372 4296
Fragments arranged by size:
4296 1372 789 708
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5776 136
Size: 107 122 71 631 605 4104 1525
Fragments arranged by size:
4104 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2413 3280 7160 2413
Size: 867 3880 2418
Fragments arranged by size:
3880 2418 867
BlpI GC'TnA_GC
Cuts at: 2519 4582 2519
Size: 2063 5102
Bme1580I G_kGCm'C
Cuts at: 1790 2983 5226 6382 1790
Size: 1193 2243 1156 2573
Fragments arranged by size:
2573 2243 1193 1156
BmgBI CAC'GTC
Cuts at: 1318 2548 1318
Size: 1230 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2907 3062 4896
Size: 416 53 83 399 24 1490 155 1834
Cuts at: 4896 5854 442
Size: 958 1753
Fragments arranged by size:
1834 1753 1490 958 416 399 155 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3932
Size: 7165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2191 2469 2489 2577 2612 4163 5825 1239
Size: 952 278 20 88 35 1551 1662 2579
Fragments arranged by size:
2579 1662 1551 952 278 88 35 20
Bpu10I CC'TnA_GC
Cuts at: 3629 4145 4151 3629
Size: 516 6 6643
Fragments arranged by size:
6643 516 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 3130 3815 3902 5290 6155 6453 2780
Size: 350 685 87 1388 865 298 3492
Fragments arranged by size:
3492 1388 865 685 350 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3576 5828 882
Size: 617 2077 2252 2219
Fragments arranged by size:
2252 2219 2077 617
BsaAI yAC'GTr
Cuts at: 493 1204 2692 3164 3328 4956 493
Size: 711 1488 472 164 1628 2702
Fragments arranged by size:
2702 1628 1488 711 472 164
BsaBI GATnn'nnATC
Cuts at: 7159 7159
Size: 7165
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3500 3737 4972 5354
Size: 53 83 186 936 1967 237 1235 382
Cuts at: 5354 5989 275
Size: 635 1451
Fragments arranged by size:
1967 1451 1235 936 635 382 237 186
83 53
BsaWI w'CCGG_w
Cuts at: 2847 3536 4187 5598 5996 6270 6417 6828 2847
Size: 689 651 1411 398 274 147 411 3184
Fragments arranged by size:
3184 1411 689 651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 2522 2552 4161
Size: 30 165 30 711 30 378 30 1609
Cuts at: 4161 4191 1178
Size: 30 4152
Fragments arranged by size:
4152 1609 711 378 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 3420 4250 1186
Size: 1111 1123 830 4101
Fragments arranged by size:
4101 1123 1111 830
BseYI C'CCAG_C
Cuts at: 2062 4317 6368 6864 2062
Size: 2255 2051 496 2363
Fragments arranged by size:
2363 2255 2051 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2570 3308 3414 3851 1163
Size: 573 834 738 106 437 4477
Fragments arranged by size:
4477 834 738 573 437 106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4050 4615 5376 6404 1540
Size: 275 186 2049 565 761 1028 2301
Fragments arranged by size:
2301 2049 1028 761 565 275 186
BsiHKAI G_wGCw'C
Cuts at: 2289 2455 5226 5311 6382 2289
Size: 166 2771 85 1071 3072
Fragments arranged by size:
3072 2771 1071 166 85
BsmI GAATG_Cn'
Cuts at: 3460 4676 4769 3460
Size: 1216 93 5856
Fragments arranged by size:
5856 1216 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2111 2209 3903 2111
Size: 98 1694 5373
Fragments arranged by size:
5373 1694 98
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 2303 2455 2983 3898 4146 4484
Size: 399 499 14 152 528 915 248 338
Cuts at: 4484 5226 5311 6382 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 915 742 528 499 399 338
248 152 85 14
BspEI T'CCGG_A
Cuts at: 2847 6828 2847
Size: 3981 3184
BspHI T'CATG_A
Cuts at: 4396 5054 6784 4396
Size: 658 1730 4777
Fragments arranged by size:
4777 1730 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3594 4520 844
Size: 2750 926 3489
Fragments arranged by size:
3489 2750 926
BsrBI CCG'CTC
Cuts at: 1394 1823 2012 3744 5052 1394
Size: 429 189 1732 1308 3507
Fragments arranged by size:
3507 1732 1308 429 189
BsrDI GCAATG_nn'
Cuts at: 65 1248 5660 5834 65
Size: 1183 4412 174 1396
Fragments arranged by size:
4412 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3536 5809 5996 1700
Size: 110 1726 2273 187 2869
Fragments arranged by size:
2869 2273 1726 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7165
BssHII G'CGCG_C
Cuts at: 1925 2055 2596 1925
Size: 130 541 6494
Fragments arranged by size:
6494 541 130
BssSI C'ACGA_G
Cuts at: 3929 4082 5225 6237 3929
Size: 153 1143 1012 4857
Fragments arranged by size:
4857 1143 1012 153
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4097 794
Size: 3303 3862
BstBI TT'CG_AA
Cuts at: 5969 5969
Size: 7165
BstEII G'GTnAC_C
Cuts at: 1184 3766 1184
Size: 2582 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 2158 3131 3727 3778 3950 2158
Size: 973 596 51 172 5373
Fragments arranged by size:
5373 973 596 172 51
BstYI r'GATC_y
Cuts at: 1972 2413 3280 3576 3687 4495 4862 5247 5264
Size: 441 867 296 111 808 367 385 17
Cuts at: 5264 6716 6802 6814 7160 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 867 808 441 385 367 346
296 111 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2262 2303 3949 3993 513
Size: 552 939 258 41 1646 44 3685
Fragments arranged by size:
3685 1646 939 552 258 44 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6426
Fragments arranged by size:
6426 683 56
BtsI GCAGTG_nn'
Cuts at: 4098 4681 5475 5495 4098
Size: 583 794 20 5768
Fragments arranged by size:
5768 794 583 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7130
DraI TTT'AAA
Cuts at: 3181 4469 3181
Size: 1288 5877
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3539 6172 809
Size: 507 2223 2633 1802
Fragments arranged by size:
2633 2223 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3936 3952
Size: 54 1425 50 275 18 177 1929 16
Cuts at: 3952 4612 5501 7130 8
Size: 660 889 1629 43
Fragments arranged by size:
1929 1629 1425 889 660 275 177 54
50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 4612 1537
Size: 275 2800 4090
Fragments arranged by size:
4090 2800 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2610 3090 3857 4292 5095 1353
Size: 756 501 480 767 435 803 3423
Fragments arranged by size:
3423 803 767 756 501 480 435
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3958 5752 6126 6272 387
Size: 1064 138 2369 1794 374 146 1280
Fragments arranged by size:
2369 1794 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2287 2287
Size: 7165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2469 2489 2577 2612 4058 4163
Size: 393 559 278 20 88 35 1446 105
Cuts at: 4163 5228 5825 6612 6880 1239
Size: 1065 597 787 268 1524
Fragments arranged by size:
1524 1446 1065 787 597 559 393 278
268 105 88 35 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 3830 4178 2333
Size: 1497 348 5320
Fragments arranged by size:
5320 1497 348
EcoO109I rG'GnC_Cy
Cuts at: 2155 3619 2155
Size: 1464 5701
EcoRI G'AATT_C
Cuts at: 4474 4852 4474
Size: 378 6787
FspI TGC'GCA
Cuts at: 2684 3884 5671 2684
Size: 1200 1787 4178
Fragments arranged by size:
4178 1787 1200
FspAI rTGC'GCAy
Cuts at: 2684 3884 2684
Size: 1200 5965
HaeII r_GCGC'y
Cuts at: 1678 3503 3740 4159 6312 1678
Size: 1825 237 419 2153 2531
Fragments arranged by size:
2531 2153 1825 419 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2184 2216 2739
Size: 32 135 32 542 32 38 32 523
Cuts at: 2739 2771 3113 3145 3231 3263 3399 3431 3779
Size: 32 342 32 86 32 136 32 348
Cuts at: 3779 3811 5806 5838 5880 5912 1373
Size: 32 1995 32 42 32 2626
Fragments arranged by size:
2626 1995 542 523 348 342 136 135
86 42 38 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2479 4051 4380 4509 4755 669
Size: 988 281 541 1572 329 129 246 3079
Fragments arranged by size:
3079 1572 988 541 329 281 246 129
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7165
HpaI GTT'AAC
Cuts at: 4380 4755 4380
Size: 375 6790
KasI G'GCGC_C
Cuts at: 3499 3736 3499
Size: 237 6928
KpnI G_GTAC'C
Cuts at: 4490 4490
Size: 7165
MfeI C'AATT_G
Cuts at: 4764 4764
Size: 7165
MluI A'CGCG_T
Cuts at: 7041 7041
Size: 7165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2682 6279 6463 1305
Size: 1377 3597 184 2007
Fragments arranged by size:
3597 2007 1377 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3954 10
Size: 54 1425 343 2122 3221
Fragments arranged by size:
3221 2122 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 3011 3269 3725 5123 5482 5641
Size: 648 1524 321 258 456 1398 359 159
Cuts at: 5641 518
Size: 2042
Fragments arranged by size:
2042 1524 1398 648 456 359 321 258
159
MspA1I CmG'CkG
Cuts at: 1606 2247 2264 2305 3615 4581 5258 6406 6651
Size: 641 17 41 1310 966 677 1148 245
Cuts at: 6651 1606
Size: 2120
Fragments arranged by size:
2120 1310 1148 966 677 641 245 41
17
NarI GG'CG_CC
Cuts at: 3500 3737 3500
Size: 237 6928
NcoI C'CATG_G
Cuts at: 513 1065 2004 3949 513
Size: 552 939 1945 3729
Fragments arranged by size:
3729 1945 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7165
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7165
NotI GC'GGCC_GC
Cuts at: 4612 4612
Size: 7165
NspI r_CATG'y
Cuts at: 1132 1229 2770 3643 4339 4523 6068 1132
Size: 97 1541 873 696 184 1545 2229
Fragments arranged by size:
2229 1545 1541 873 696 184 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7165
PciI A'CATG_T
Cuts at: 3639 6064 3639
Size: 2425 4740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7165
PfoI T'CCnGG_A
Cuts at: 2446 3942 4179 2446
Size: 1496 237 5432
Fragments arranged by size:
5432 1496 237
PmeI GTTT'AAAC
Cuts at: 4469 4469
Size: 7165
PmlI CAC'GTG
Cuts at: 2692 3164 3328 2692
Size: 472 164 6529
Fragments arranged by size:
6529 472 164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3280 3312 5234 5266 6747 6779 3280
Size: 32 1922 32 1481 32 3666
Fragments arranged by size:
3666 1922 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 3619 2155
Size: 1464 5701
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7165
PsiI TTA'TAA
Cuts at: 4735 4735
Size: 7165
PstI C_TGCA'G
Cuts at: 830 3495 3591 4257 4517 830
Size: 2665 96 666 260 3478
Fragments arranged by size:
3478 2665 666 260 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7165
SacI G_AGCT'C
Cuts at: 2289 2289
Size: 7165
SacII CC_GC'GG
Cuts at: 2265 2306 2265
Size: 41 7124
SalI G'TCGA_C
Cuts at: 1936 4049 4507 1936
Size: 2113 458 4594
Fragments arranged by size:
4594 2113 458
SanDI GG'GwC_CC
Cuts at: 3619 3619
Size: 7165
SbfI CC_TGCA'GG
Cuts at: 4517 4517
Size: 7165
ScaI AGT'ACT
Cuts at: 5413 7125 5413
Size: 1712 5453
SexAI A'CCwGG_T
Cuts at: 2631 3762 2631
Size: 1131 6034
SfcI C'TryA_G
Cuts at: 826 1045 3491 3587 4253 4513 5648 6329 6520
Size: 219 2446 96 666 260 1135 681 191
Cuts at: 6520 826
Size: 1471
Fragments arranged by size:
2446 1471 1135 681 666 260 219 191
96
SfoI GGC'GCC
Cuts at: 3501 3738 3501
Size: 237 6928
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7165
SgrAI Cr'CCGG_yG
Cuts at: 1700 3536 1700
Size: 1836 5329
SmaI CCC'GGG
Cuts at: 1872 3091 4492 1872
Size: 1219 1401 4545
Fragments arranged by size:
4545 1401 1219
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2759 3109 3830 3917 5269 6170
Size: 197 926 816 350 721 87 1352 901
Cuts at: 6170 6432 820
Size: 262 1553
Fragments arranged by size:
1553 1352 926 901 816 721 350 262
197 87
SnaBI TAC'GTA
Cuts at: 493 4956 493
Size: 4463 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7165
SphI G_CATG'C
Cuts at: 1132 4523 1132
Size: 3391 3774
SspI AAT'ATT
Cuts at: 5 52 2320 3230 5089 5
Size: 47 2268 910 1859 2081
Fragments arranged by size:
2268 2081 1859 910 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7165
StyI C'CwwG_G
Cuts at: 513 1065 2004 2151 3771 3949 4604 513
Size: 552 939 147 1620 178 655 3074
Fragments arranged by size:
3074 1620 939 655 552 178 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3252 3494 4036 4037 4217 5192 5209
Size: 1255 1672 242 542 1 180 975 17
Cuts at: 5209 5362 5547 5992 325
Size: 153 185 445 1498
Fragments arranged by size:
1672 1498 1255 975 542 445 242 185
180 153 17 1
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5411 7123 96
Size: 274 80 33 51 1443 3434 1712 138
Fragments arranged by size:
3434 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2522 2554 4161 4193 7125 7157 2522
Size: 32 1607 32 2932 32 2530
Fragments arranged by size:
2932 2530 1607 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7165
XbaI T'CTAG_A
Cuts at: 2416 2923 4501 2416
Size: 507 1578 5080
Fragments arranged by size:
5080 1578 507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4187 4187
Size: 7165
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7165
XmaI C'CCGG_G
Cuts at: 1870 3089 4490 1870
Size: 1219 1401 4545
Fragments arranged by size:
4545 1401 1219
XmnI GAAnn'nnTTC
Cuts at: 5294 5294
Size: 7165
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4973 276
Size: 53 83 186 936 3439 2468
Fragments arranged by size:
3439 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI NaeI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SapI SfiI SrfI SwaI