Restriction map for
pFN21AA0325
(Circular) (Six-base) MAPSORT of: pFN21AA0325.seq Check: 4745 from: 1 to: 18643
pFN21AA0325 18643 bp
With 182 enzymes: SgfI *
May 20, 2014 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2112 5698 5953 6690 7915 13948 14268 2112
Size: 3586 255 737 1225 6033 320 6487
Fragments arranged by size:
6487 6033 3586 1225 737 320 255
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2268 16453 278
Size: 53 83 186 936 732 14185 2468
Fragments arranged by size:
14185 2468 936 732 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5478 6639 10656 11640 14868 15831 15986 1937
Size: 3541 1161 4017 984 3228 963 155 4594
Fragments arranged by size:
4594 4017 3541 3228 1161 984 963 155
Acc65I G'GTAC_C
Cuts at: 5013 15964 5013
Size: 10951 7692
AclI AA'CG_TT
Cuts at: 1563 16770 17143 1563
Size: 15207 373 3063
Fragments arranged by size:
15207 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2529 3278 3362 4070 4815 5153 5160 5819
Size: 897 749 84 708 745 338 7 659
Cuts at: 5819 5894 8211 8461 8570 8738 9805 9920 10589
Size: 75 2317 250 109 168 1067 115 669
Cuts at: 10589 10715 12461 12844 13343 13562 13700 14312 15056
Size: 126 1746 383 499 219 138 612 744
Cuts at: 15056 15260 15433 16706 18090 18358 1632
Size: 204 173 1273 1384 268 1917
Fragments arranged by size:
2317 1917 1746 1384 1273 1067 897 749
745 744 708 669 659 612 499 383
338 268 250 219 204 173 168 138
126 115 109 84 75 7
AfeI AGC'GCT
Cuts at: 2246 2930 4508 5588 9467 10431 2246
Size: 684 1578 1080 3879 964 10458
Fragments arranged by size:
10458 3879 1578 1080 964 684
AflII C'TTAA_G
Cuts at: 820 1017 4202 820
Size: 197 3185 15261
Fragments arranged by size:
15261 3185 197
AflIII A'CryG_T
Cuts at: 1203 2082 8493 8848 12117 12216 14022 14164 14664
Size: 879 6411 355 3269 99 1806 142 500
Cuts at: 14664 15528 17542 18519 1203
Size: 864 2014 977 1327
Fragments arranged by size:
6411 3269 2014 1806 1327 977 879 864
500 355 142 99
AgeI A'CCGG_T
Cuts at: 17474 17474
Size: 18643
AhdI GACnn_n'nnGTC
Cuts at: 3853 14641 15109 17372 3853
Size: 10788 468 2263 5124
Fragments arranged by size:
10788 5124 2263 468
AleI CACnn'nnGTG
Cuts at: 1166 3795 3918 5519 8520 8849 9572 14105 14602
Size: 2629 123 1601 3001 329 723 4533 497
Cuts at: 14602 1166
Size: 5207
Fragments arranged by size:
5207 4533 3001 2629 1601 723 497 329
123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 12266 14376 15949 1184
Size: 948 10134 2110 1573 3878
Fragments arranged by size:
10134 3878 2110 1573 948
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4391 4423 14804 14836 15215 15247 4391
Size: 32 10381 32 379 32 7787
Fragments arranged by size:
10381 7787 379 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2813 4048 4447 4520 5930 7388 7748 9698 10157
Size: 1235 399 73 1410 1458 360 1950 459
Cuts at: 10157 10629 11914 12259 13570 13783 14794 15083 15187
Size: 472 1285 345 1311 213 1011 289 104
Cuts at: 15187 16386 17958 2813
Size: 1199 1572 3498
Fragments arranged by size:
3498 1950 1572 1458 1410 1311 1285 1235
1199 1011 472 459 399 360 345 289
213 104 73
ApaI G_GGCC'C
Cuts at: 8679 10226 8679
Size: 1547 17096
ApaLI G'TGCA_C
Cuts at: 12879 15934 16700 17856 12879
Size: 3055 766 1156 13666
Fragments arranged by size:
13666 3055 1156 766
ApoI r'AATT_y
Cuts at: 1493 2294 2607 2769 3227 3407 5054 5393 5426
Size: 801 313 162 458 180 1647 339 33
Cuts at: 5426 6088 6386 7598 11327 12797 13214 13715 14621
Size: 662 298 1212 3729 1470 417 501 906
Cuts at: 14621 15952 16178 16330 18439 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
3729 2109 1697 1647 1470 1331 1212 906
801 662 501 458 417 339 313 298
226 180 162 152 33
AseI AT'TA_AT
Cuts at: 160 1032 17197 160
Size: 872 16165 1606
Fragments arranged by size:
16165 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18643
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 4144 4173 7038 8680 8735 10219
Size: 73 72 2129 29 2865 1642 55 1484
Cuts at: 10219 12995 13047 13086 13620 15968 1870
Size: 2776 52 39 534 2348 4545
Fragments arranged by size:
4545 2865 2776 2348 2129 1642 1484 534
73 72 55 52 39 29
BamHI G'GATC_C
Cuts at: 2760 4998 8926 13051 13773 15223 15895 15973 16340
Size: 2238 3928 4125 722 1450 672 78 367
Cuts at: 16340 2760
Size: 5063
Fragments arranged by size:
5063 4125 3928 2238 1450 722 672 367
78
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2146 5013 7859 8440
Size: 325 217 627 329 30 2867 2846 581
Cuts at: 8440 9658 10250 11053 13685 14404 15120 15409 15503
Size: 1218 592 803 2632 719 716 289 94
Cuts at: 15503 15653 15964 17419 618
Size: 150 311 1455 1842
Fragments arranged by size:
2867 2846 2632 1842 1455 1218 803 719
716 627 592 581 329 325 311 289
217 150 94 30
BanII G_rGCy'C
Cuts at: 1391 2016 2888 5571 8033 8679 8841 9374 10226
Size: 625 872 2683 2462 646 162 533 852
Cuts at: 10226 11154 11619 12237 13701 15621 15962 1391
Size: 928 465 618 1464 1920 341 4072
Fragments arranged by size:
4072 2683 2462 1920 1464 928 872 852
646 625 618 533 465 341 162
BbeI G_GCGC'C
Cuts at: 2150 15124 15507 15657 2150
Size: 12974 383 150 5136
Fragments arranged by size:
12974 5136 383 150
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2391 2436 2721 4516 5798 8264 8902
Size: 971 492 45 285 1795 1282 2466 638
Cuts at: 8902 8999 9908 10920 12674 15098 928
Size: 97 909 1012 1754 2424 4473
Fragments arranged by size:
4473 2466 2424 1795 1754 1282 1012 971
909 638 492 285 97 45
BbvCI CC'TCA_GC
Cuts at: 3528 10271 10763 14477 3528
Size: 6743 492 3714 7694
Fragments arranged by size:
7694 6743 3714 492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6925 6959 10544 10578 14159 14193 16834
Size: 34 4988 34 3585 34 3581 34 2641
Cuts at: 16834 16868 1903
Size: 34 3678
Fragments arranged by size:
4988 3678 3585 3581 2641 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10357 12771 16535 17745 10357
Size: 2414 3764 1210 11255
Fragments arranged by size:
11255 3764 2414 1210
BclI T'GATC_A
Cuts at: 1548 2640 4557 7479 9910 12768 12987 15021 15357
Size: 1092 1917 2922 2431 2858 219 2034 336
Cuts at: 15357 1548
Size: 4834
Fragments arranged by size:
4834 2922 2858 2431 2034 1917 1092 336
219
BfrBI ATG'CAT
Cuts at: 3211 3211
Size: 18643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3644 5909 7694
Size: 107 122 71 631 605 1972 2265 1785
Cuts at: 7694 8140 9833 12389 14962 15540 17254 136
Size: 446 1693 2556 2573 578 1714 1525
Fragments arranged by size:
2573 2556 2265 1972 1785 1714 1693 1525
631 605 578 446 122 107 71
BglII A'GATC_T
Cuts at: 4095 5227 5439 10242 10927 11845 13075 18638 4095
Size: 1132 212 4803 685 918 1230 5563 4100
Fragments arranged by size:
5563 4803 4100 1230 1132 918 685 212
BlpI GC'TnA_GC
Cuts at: 4510 7349 7520 9110 13817 15188 16060 4510
Size: 2839 171 1590 4707 1371 872 7093
Fragments arranged by size:
7093 4707 2839 1590 1371 872 171
Bme1580I G_kGCm'C
Cuts at: 1790 3136 3197 3632 8679 10226 10483 11116 12883
Size: 1346 61 435 5047 1547 257 633 1767
Cuts at: 12883 13688 13917 14102 14536 15938 16704 17860 1790
Size: 805 229 185 434 1402 766 1156 2573
Fragments arranged by size:
5047 2573 1767 1547 1402 1346 1156 805
766 633 435 434 257 229 185 61
BmgBI CAC'GTC
Cuts at: 1318 2083 8494 9288 11194 15111 1318
Size: 765 6411 794 1906 3917 4850
Fragments arranged by size:
6411 4850 3917 1906 794 765
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4117 5794 5940
Size: 416 53 83 399 24 2700 1677 146
Cuts at: 5940 7743 10646 14609 14743 16374 17332 442
Size: 1803 2903 3963 134 1631 958 1753
Fragments arranged by size:
3963 2903 2700 1803 1753 1677 1631 958
416 399 146 134 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 11489 1056
Size: 10433 8210
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2056 5246 5488 7030 8260 9017 12450 2056
Size: 3190 242 1542 1230 757 3433 8249
Fragments arranged by size:
8249 3433 3190 1542 1230 757 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2180 2192 2210 2766 3745 7002 7913
Size: 911 30 12 18 556 979 3257 911
Cuts at: 7913 8523 8612 9353 9482 9534 9856 9870 10133
Size: 610 89 741 129 52 322 14 263
Cuts at: 10133 10463 11039 11369 11606 11711 11987 13334 13475
Size: 330 576 330 237 105 276 1347 141
Cuts at: 13475 13945 14351 14831 15632 17303 1239
Size: 470 406 480 801 1671 2579
Fragments arranged by size:
3257 2579 1671 1347 979 911 911 801
741 610 576 556 480 470 406 330
330 322 276 263 237 141 129 105
89 52 30 18 14 12
Bpu10I CC'TnA_GC
Cuts at: 3528 7548 10271 10763 11618 14477 3528
Size: 4020 2723 492 855 2859 7694
Fragments arranged by size:
7694 4020 2859 2723 855 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3187 10776 11213 11689 13442 13750 15182 16768 17633
Size: 7589 437 476 1753 308 1432 1586 865
Cuts at: 17633 17931 3187
Size: 298 3899
Fragments arranged by size:
7589 3899 1753 1586 1432 865 476 437
308 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3680 6807 7286 9645 9866 10429 10439
Size: 617 2181 3127 479 2359 221 563 10
Cuts at: 10439 11066 12312 13382 17306 882
Size: 627 1246 1070 3924 2219
Fragments arranged by size:
3924 3127 2359 2219 2181 1246 1070 627
617 563 479 221 10
BsaAI yAC'GTr
Cuts at: 493 1204 4033 8851 10195 10759 14667 15529 15711
Size: 711 2829 4818 1344 564 3908 862 182
Cuts at: 15711 16434 493
Size: 723 2702
Fragments arranged by size:
4818 3908 2829 2702 1344 862 723 711
564 182
BsaBI GATnn'nnATC
Cuts at: 2796 8925 10926 11513 18637 2796
Size: 6129 2001 587 7124 2802
Fragments arranged by size:
7124 6129 2802 2001 587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2265 3415 4740
Size: 53 83 186 936 614 118 1150 1325
Cuts at: 4740 12087 12270 12855 14954 15121 15504 15654 16450
Size: 7347 183 585 2099 167 383 150 796
Cuts at: 16450 16832 17467 275
Size: 382 635 1451
Fragments arranged by size:
7347 2099 1451 1325 1150 936 796 635
614 585 383 382 186 183 167 150
118 83 53
BsaWI w'CCGG_w
Cuts at: 2611 4254 8112 12423 14707 17076 17474 17748 17895
Size: 1643 3858 4311 2284 2369 398 274 147
Cuts at: 17895 18306 2611
Size: 411 2948
Fragments arranged by size:
4311 3858 2948 2369 2284 1643 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4813 4843 4974 5004 7038
Size: 30 165 30 3410 30 131 30 2034
Cuts at: 7038 7043 7068 7073 8583 8613 11912 11942 13379
Size: 5 25 5 1510 30 3299 30 1437
Cuts at: 13379 13409 13469 13499 13880 13910 14324 14354 14804
Size: 30 60 30 381 30 414 30 450
Cuts at: 14804 14834 1178
Size: 30 4987
Fragments arranged by size:
4987 3410 3299 2034 1510 1437 450 414
381 165 131 60 30 30 30 30
30 30 30 30 30 30 30 25
5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 4406 7064 7910 8558 9236 10541 11162
Size: 1003 2217 2658 846 648 678 1305 621
Cuts at: 11162 11603 12089 13373 14927 15629 1186
Size: 441 486 1284 1554 702 4200
Fragments arranged by size:
4200 2658 2217 1554 1305 1284 1003 846
702 678 648 621 486 441
BseYI C'CCAG_C
Cuts at: 3822 4020 6471 6570 6699 7245 7702 7920 9544
Size: 198 2451 99 129 546 457 218 1624
Cuts at: 9544 11986 12515 13398 14965 15777 17846 18342 3822
Size: 2442 529 883 1567 812 2069 496 4123
Fragments arranged by size:
4123 2451 2442 2069 1624 1567 883 812
546 529 496 457 218 198 129 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 4142 4460 4658 4957 5543 5981
Size: 573 351 2055 318 198 299 586 438
Cuts at: 5981 6644 6687 7949 8322 9374 14712 14942 15128
Size: 663 43 1262 373 1052 5338 230 186
Cuts at: 15128 15152 15870 1163
Size: 24 718 3936
Fragments arranged by size:
5338 3936 2055 1262 1052 718 663 586
573 438 373 351 318 299 230 198
186 43 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 2064 2154 8140 9573 14002
Size: 275 186 45 18 90 5986 1433 4429
Cuts at: 14002 16093 16854 17882 1540
Size: 2091 761 1028 2301
Fragments arranged by size:
5986 4429 2301 2091 1433 1028 761 275
186 90 45 18
BsiHKAI G_wGCw'C
Cuts at: 5571 9116 9321 9339 10071 11154 12431 12883 14138
Size: 3545 205 18 732 1083 1277 452 1255
Cuts at: 14138 15621 15938 16704 16789 17860 5571
Size: 1483 317 766 85 1071 6354
Fragments arranged by size:
6354 3545 1483 1277 1255 1083 1071 766
732 452 317 205 85 18
BsmI GAATG_Cn'
Cuts at: 2354 2452 3739 5071 11482 11707 11890 14344 14493
Size: 98 1287 1332 6411 225 183 2454 149
Cuts at: 14493 16154 16247 2354
Size: 1661 93 4750
Fragments arranged by size:
6411 4750 2454 1661 1332 1287 225 183
149 98 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6520 8426 9671 10502 10939 11067 13355 14506 6520
Size: 1906 1245 831 437 128 2288 1151 10657
Fragments arranged by size:
10657 2288 1906 1245 1151 831 437 128
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2888 3136 3197 3632 5571 8033
Size: 399 226 872 248 61 435 1939 2462
Cuts at: 8033 8679 8841 9116 9321 9339 9374 10071 10226
Size: 646 162 275 205 18 35 697 155
Cuts at: 10226 10483 11116 11154 11619 12237 12431 12883 13688
Size: 257 633 38 465 618 194 452 805
Cuts at: 13688 13701 13917 14102 14138 14536 15621 15938 15962
Size: 13 216 185 36 398 1085 317 24
Cuts at: 15962 16704 16789 17860 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2462 2174 1939 1085 1071 872 805 742
697 646 633 618 465 452 435 399
398 317 275 257 248 226 216 205
194 185 162 155 85 61 38 36
35 24 18 13
BspEI T'CCGG_A
Cuts at: 2611 4254 8112 18306 2611
Size: 1643 3858 10194 2948
Fragments arranged by size:
10194 3858 2948 1643
BspHI T'CATG_A
Cuts at: 4882 6252 16532 18262 4882
Size: 1370 10280 1730 5263
Fragments arranged by size:
10280 5263 1730 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2212 4385 4765 5698 5727 5781 5953
Size: 1268 100 2173 380 933 29 54 172
Cuts at: 5953 6690 6831 7732 7915 11872 13948 14268 14646
Size: 737 141 901 183 3957 2076 320 378
Cuts at: 14646 15998 844
Size: 1352 3489
Fragments arranged by size:
3957 3489 2173 2076 1352 1268 933 901
737 380 378 320 183 172 141 100
54 29
BsrBI CCG'CTC
Cuts at: 1394 1823 3701 4396 6871 7900 10309 12021 13414
Size: 429 1878 695 2475 1029 2409 1712 1393
Cuts at: 13414 14077 15916 16530 1394
Size: 663 1839 614 3507
Fragments arranged by size:
3507 2475 2409 1878 1839 1712 1393 1029
695 663 614 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2473 4461 5584 7360 7830 8314 10608
Size: 1183 1225 1988 1123 1776 470 484 2294
Cuts at: 10608 12250 12510 12543 17138 17312 65
Size: 1642 260 33 4595 174 1396
Fragments arranged by size:
4595 2294 1988 1776 1642 1396 1225 1183
1123 484 470 260 174 33
BsrFI r'CCGG_y
Cuts at: 1700 1810 2149 8076 8139 9522 15416 17287 17474
Size: 110 339 5927 63 1383 5894 1871 187
Cuts at: 17474 1700
Size: 2869
Fragments arranged by size:
5927 5894 2869 1871 1383 339 187 110
63
BsrGI T'GTAC_A
Cuts at: 96 4501 5026 9747 10620 96
Size: 4405 525 4721 873 8119
Fragments arranged by size:
8119 4721 4405 873 525
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 18643
BssSI C'ACGA_G
Cuts at: 11144 11567 16703 17715 11144
Size: 423 5136 1012 12072
Fragments arranged by size:
12072 5136 1012 423
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6685 7345 11599 13912 14350 794
Size: 5891 660 4254 2313 438 5087
Fragments arranged by size:
5891 5087 4254 2313 660 438
BstBI TT'CG_AA
Cuts at: 15876 17447 15876
Size: 1571 17072
BstEII G'GTnAC_C
Cuts at: 1184 7363 9301 12893 13342 15817 1184
Size: 6179 1938 3592 449 2475 4010
Fragments arranged by size:
6179 4010 3592 2475 1938 449
BstXI CCAn_nnnn'nTGG
Cuts at: 2350 3052 5625 7772 9298 10130 12301 12847 13663
Size: 702 2573 2147 1526 832 2171 546 816
Cuts at: 13663 14604 2350
Size: 941 6389
Fragments arranged by size:
6389 2573 2171 2147 1526 941 832 816
702 546
BstYI r'GATC_y
Cuts at: 1972 2760 2797 3102 4095 4998 5227 5439 6834
Size: 788 37 305 993 903 229 212 1395
Cuts at: 6834 7833 8926 9246 10242 10927 11344 11845 12015
Size: 999 1093 320 996 685 417 501 170
Cuts at: 12015 13051 13075 13773 14715 15223 15378 15895 15973
Size: 1036 24 698 942 508 155 517 78
Cuts at: 15973 16340 16725 16742 18194 18280 18292 18638 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1395 1093 1036 999 996 993
942 903 788 698 685 517 508 501
417 385 367 346 320 305 229 212
170 155 86 78 37 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 3455 15555 3455
Size: 12100 6543
BtgI C'CryG_G
Cuts at: 513 1065 3045 3796 5751 6466 9644 10320 11931
Size: 552 1980 751 1955 715 3178 676 1611
Cuts at: 11931 13446 13689 14013 14385 14720 15207 15867 513
Size: 1515 243 324 372 335 487 660 3289
Fragments arranged by size:
3289 3178 1980 1955 1611 1515 751 715
676 660 552 487 372 335 324 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 8247 8934 12319 487
Size: 683 56 7021 687 3385 6811
Fragments arranged by size:
7021 6811 3385 687 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 3134 4662 5508 5953 6670 7937 7946 8184
Size: 656 1528 846 445 717 1267 9 238
Cuts at: 8184 9585 10311 11105 12429 14930 15140 15567 16159
Size: 1401 726 794 1324 2501 210 427 592
Cuts at: 16159 16953 16973 2478
Size: 794 20 4148
Fragments arranged by size:
4148 2501 1528 1401 1324 1267 846 794
794 726 717 656 592 445 427 238
210 20 9
ClaI AT'CG_AT
Cuts at: 1554 6033 9609 1554
Size: 4479 3576 10588
Fragments arranged by size:
10588 4479 3576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4834 4869 8433 8468 529
Size: 35 4270 35 3564 35 10704
Fragments arranged by size:
10704 4270 3564 35 35 35
DraI TTT'AAA
Cuts at: 7063 8224 15947 7063
Size: 1161 7723 9759
Fragments arranged by size:
9759 7723 1161
DraIII CAC_nnn'GTG
Cuts at: 2233 2426 7345 7952 9688 10394 11197 11917 14095
Size: 193 4919 607 1736 706 803 720 2178
Cuts at: 14095 15011 2233
Size: 916 5865
Fragments arranged by size:
5865 4919 2178 1736 916 803 720 706
607 193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2222 4583 7640 17650 809
Size: 507 906 2361 3057 10010 1802
Fragments arranged by size:
10010 3057 2361 1802 906 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2061 2151
Size: 54 1425 50 275 18 213 18 90
Cuts at: 2151 5484 5581 6298 6519 7143 7762 8040 8121
Size: 3333 97 717 221 624 619 278 81
Cuts at: 8121 9063 9141 9759 11040 11511 11934 13954 14010
Size: 942 78 618 1281 471 423 2020 56
Cuts at: 14010 14682 16090 16979 18608 8
Size: 672 1408 889 1629 43
Fragments arranged by size:
3333 2020 1629 1425 1408 1281 942 889
717 672 624 619 618 471 423 278
275 221 213 97 90 81 78 56
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2043 2061 2151 16090 1537
Size: 275 231 18 90 13939 4090
Fragments arranged by size:
13939 4090 275 231 90 18
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 3650 3864 4926 5043 5100 5820 5946
Size: 821 1476 214 1062 117 57 720 126
Cuts at: 5946 6488 6549 8251 8279 8309 8480 8687 8846
Size: 542 61 1702 28 30 171 207 159
Cuts at: 8846 9383 9824 10505 10659 11469 12437 12441 13319
Size: 537 441 681 154 810 968 4 878
Cuts at: 13319 13598 14381 16573 1353
Size: 279 783 2192 3423
Fragments arranged by size:
3423 2192 1702 1476 1062 968 878 821
810 783 720 681 542 537 441 279
214 207 171 159 154 126 117 61
57 30 28 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 7329 7435 9719 13814 17230
Size: 1064 138 504 5236 106 2284 4095 3416
Cuts at: 17230 17604 17750 387
Size: 374 146 1280
Fragments arranged by size:
5236 4095 3416 2284 1280 1064 504 374
146 138 106
EcoICRI GAG'CTC
Cuts at: 5569 11152 15619 5569
Size: 5583 4467 8593
Fragments arranged by size:
8593 5583 4467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2180 2192 2210 2529 2766 3278
Size: 393 518 30 12 18 319 237 512
Cuts at: 3278 3362 3745 4070 4815 5153 5160 5819 5894
Size: 84 383 325 745 338 7 659 75
Cuts at: 5894 7002 7913 8211 8461 8523 8570 8612 8738
Size: 1108 911 298 250 62 47 42 126
Cuts at: 8738 9353 9482 9534 9805 9856 9870 9920 10133
Size: 615 129 52 271 51 14 50 213
Cuts at: 10133 10463 10589 10715 11039 11369 11606 11711 11987
Size: 330 126 126 324 330 237 105 276
Cuts at: 11987 12461 12844 13334 13343 13475 13562 13700 13945
Size: 474 383 490 9 132 87 138 245
Cuts at: 13945 14312 14351 14831 15056 15260 15433 15632 16706
Size: 367 39 480 225 204 173 199 1074
Cuts at: 16706 17303 18090 18358 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1108 1074 911 787 745 659 615
597 518 512 490 480 474 393 383
383 367 338 330 330 325 324 319
298 276 271 268 250 245 237 237
225 213 204 199 173 138 132 129
126 126 126 105 87 84 75 62
52 51 50 47 42 39 30 18
14 12 9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2805 3936 4013 7004 7850 8540 8717 10458 11918
Size: 1131 77 2991 846 690 177 1741 1460
Cuts at: 11918 13595 13982 2805
Size: 1677 387 7466
Fragments arranged by size:
7466 2991 1741 1677 1460 1131 846 690
387 177 77
EcoO109I rG'GnC_Cy
Cuts at: 2413 3451 3499 4444 5986 7473 8675 8676 10029
Size: 1038 48 945 1542 1487 1202 1 1353
Cuts at: 10029 10223 13588 13780 14173 15292 2413
Size: 194 3365 192 393 1119 5764
Fragments arranged by size:
5764 3365 1542 1487 1353 1202 1119 1038
945 393 194 192 48 1
EcoRI G'AATT_C
Cuts at: 2294 5426 11327 12797 13715 15952 16330 2294
Size: 3132 5901 1470 918 2237 378 4607
Fragments arranged by size:
5901 4607 3132 2237 1470 918 378
EcoRV GAT'ATC
Cuts at: 2911 6673 9482 2911
Size: 3762 2809 12072
Fragments arranged by size:
12072 3762 2809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3593 4025 5371 5849 6475 6932 10733 11864 12504
Size: 432 1346 478 626 457 3801 1131 640
Cuts at: 12504 13609 3593
Size: 1105 8627
Fragments arranged by size:
8627 3801 1346 1131 1105 640 626 478
457 432
FspI TGC'GCA
Cuts at: 2108 2201 9152 15350 17149 2108
Size: 93 6951 6198 1799 3602
Fragments arranged by size:
6951 6198 3602 1799 93
HaeII r_GCGC'y
Cuts at: 1678 2150 2171 2187 2248 2932 3074 4510 5590
Size: 472 21 16 61 684 142 1436 1080
Cuts at: 5590 5915 8899 9001 9314 9469 9828 10433 11966
Size: 325 2984 102 313 155 359 605 1533
Cuts at: 11966 13570 14818 15124 15507 15657 17790 1678
Size: 1604 1248 306 383 150 2133 2531
Fragments arranged by size:
2984 2531 2133 1604 1533 1436 1248 1080
684 605 472 383 359 325 313 306
155 150 142 102 61 21 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2789 2821 3023 3055 3300
Size: 32 135 32 1217 32 202 32 245
Cuts at: 3300 3332 3426 3458 3685 3717 3839 3871 4990
Size: 32 94 32 227 32 122 32 1119
Cuts at: 4990 5022 6583 6615 6860 6892 7967 7999 8310
Size: 32 1561 32 245 32 1075 32 311
Cuts at: 8310 8342 9562 9594 9895 9927 10665 10697 11912
Size: 32 1220 32 301 32 738 32 1215
Cuts at: 11912 11944 12972 13004 13172 13204 13379 13411 13880
Size: 32 1028 32 168 32 175 32 469
Cuts at: 13880 13912 14364 14396 14627 14659 15095 15127 15370
Size: 32 452 32 231 32 436 32 243
Cuts at: 15370 15402 17284 17316 17358 17390 1373
Size: 32 1882 32 42 32 2626
Fragments arranged by size:
2626 1882 1561 1220 1217 1215 1119 1075
1028 738 469 452 436 311 301 245
245 243 231 227 202 175 168 135
122 94 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2752 4216 4705 4813 6316 10787
Size: 988 281 814 1464 489 108 1503 4471
Cuts at: 10787 11641 12061 14869 15634 15987 16233 669
Size: 854 420 2808 765 353 246 3079
Fragments arranged by size:
4471 3079 2808 1503 1464 988 854 814
765 489 420 353 281 246 108
HindIII A'AGCT_T
Cuts at: 748 4709 6077 6118 748
Size: 3961 1368 41 13273
Fragments arranged by size:
13273 3961 1368 41
HpaI GTT'AAC
Cuts at: 2752 4216 16233 2752
Size: 1464 12017 5162
Fragments arranged by size:
12017 5162 1464
KasI G'GCGC_C
Cuts at: 2146 15120 15503 15653 2146
Size: 12974 383 150 5136
Fragments arranged by size:
12974 5136 383 150
KpnI G_GTAC'C
Cuts at: 5017 15968 5017
Size: 10951 7692
MfeI C'AATT_G
Cuts at: 4926 7200 12192 14208 16242 4926
Size: 2274 4992 2016 2034 7327
Fragments arranged by size:
7327 4992 2274 2034 2016
MluI A'CGCG_T
Cuts at: 18519 18519
Size: 18643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 5442 7986 8469 9315 10517 13079 14395
Size: 684 3453 2544 483 846 1202 2562 1316
Cuts at: 14395 15030 15138 15183 15468 17757 17941 1305
Size: 635 108 45 285 2289 184 2007
Fragments arranged by size:
3453 2562 2544 2289 2007 1316 1202 846
684 635 483 285 184 108 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5583 6300 7145 7764 8042
Size: 54 1425 343 3751 717 845 619 278
Cuts at: 8042 8123 9065 9143 11042 11513 11936 13956 14012
Size: 81 942 78 1899 471 423 2020 56
Cuts at: 14012 10
Size: 4641
Fragments arranged by size:
4641 3751 2020 1899 1425 942 845 717
619 471 423 343 278 81 78 56
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3795 3918 5519 8054 8520 8849 9119
Size: 648 2629 123 1601 2535 466 329 270
Cuts at: 9119 9572 9851 11090 12029 13373 14105 14438 14509
Size: 453 279 1239 939 1344 732 333 71
Cuts at: 14509 14602 14888 15527 16601 16960 17119 518
Size: 93 286 639 1074 359 159 2042
Fragments arranged by size:
2629 2535 2042 1601 1344 1239 1074 939
732 648 639 466 453 359 333 329
286 279 270 159 123 93 71
MspA1I CmG'CkG
Cuts at: 1606 2122 2757 3826 3976 6112 6570 6703 7665
Size: 516 635 1069 150 2136 458 133 962
Cuts at: 7665 8470 9163 9190 9331 9460 9630 10729 11442
Size: 805 693 27 141 129 170 1099 713
Cuts at: 11442 11794 12404 12536 12766 13237 14264 14329 15469
Size: 352 610 132 230 471 1027 65 1140
Cuts at: 15469 15491 15603 15772 16059 16736 17884 18129 1606
Size: 22 112 169 287 677 1148 245 2120
Fragments arranged by size:
2136 2120 1148 1140 1099 1069 1027 962
805 713 693 677 635 610 516 471
458 352 287 245 230 170 169 150
141 133 132 129 112 65 27 22
NaeI GCC'GGC
Cuts at: 2151 9524 15418 2151
Size: 7373 5894 5376
Fragments arranged by size:
7373 5894 5376
NarI GG'CG_CC
Cuts at: 2147 15121 15504 15654 2147
Size: 12974 383 150 5136
Fragments arranged by size:
12974 5136 383 150
NcoI C'CATG_G
Cuts at: 513 1065 10320 11931 13446 14013 14385 513
Size: 552 9255 1611 1515 567 372 4771
Fragments arranged by size:
9255 4771 1611 1515 567 552 372
NdeI CA'TA_TG
Cuts at: 387 4498 8581 387
Size: 4111 4083 10449
Fragments arranged by size:
10449 4111 4083
NgoMIV G'CCGG_C
Cuts at: 2149 9522 15416 2149
Size: 7373 5894 5376
Fragments arranged by size:
7373 5894 5376
NheI G'CTAG_C
Cuts at: 1052 11485 1052
Size: 10433 8210
NotI GC'GGCC_GC
Cuts at: 16090 16090
Size: 18643
NruI TCG'CGA
Cuts at: 3810 3810
Size: 18643
NsiI A_TGCA'T
Cuts at: 3213 3213
Size: 18643
NspI r_CATG'y
Cuts at: 1132 1229 3215 3252 11383 12121 12220 13630 14026
Size: 97 1986 37 8131 738 99 1410 396
Cuts at: 14026 14168 14363 14476 14862 16001 17546 1132
Size: 142 195 113 386 1139 1545 2229
Fragments arranged by size:
8131 2229 1986 1545 1410 1139 738 396
386 195 142 113 99 97 37
PasI CC'CwG_GG
Cuts at: 1348 6054 14312 1348
Size: 4706 8258 5679
Fragments arranged by size:
8258 5679 4706
PciI A'CATG_T
Cuts at: 12117 12216 14022 14164 17542 12117
Size: 99 1806 142 3378 13218
Fragments arranged by size:
13218 3378 1806 142 99
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5461 7252 7825 8826 9501 11533 11689 12006
Size: 4353 1791 573 1001 675 2032 156 317
Cuts at: 12006 12916 14095 15603 1108
Size: 910 1179 1508 4148
Fragments arranged by size:
4353 4148 2032 1791 1508 1179 1001 910
675 573 317 156
PfoI T'CCnGG_A
Cuts at: 2868 5736 11016 11784 12846 12909 13500 14808 2868
Size: 2868 5280 768 1062 63 591 1308 6703
Fragments arranged by size:
6703 5280 2868 1308 1062 768 591 63
PmeI GTTT'AAAC
Cuts at: 15947 15947
Size: 18643
PmlI CAC'GTG
Cuts at: 4033 8851 10195 10759 14667 15529 15711 4033
Size: 4818 1344 564 3908 862 182 6965
Fragments arranged by size:
6965 4818 3908 1344 862 564 182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8936 8968 11201 11233 14804 14836 16712 16744 18225
Size: 32 2233 32 3571 32 1876 32 1481
Cuts at: 18225 18257 8936
Size: 32 9322
Fragments arranged by size:
9322 3571 2233 1876 1481 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2413 3451 3499 5986 7473 10029 13588 14173 15292
Size: 1038 48 2487 1487 2556 3559 585 1119
Cuts at: 15292 2413
Size: 5764
Fragments arranged by size:
5764 3559 2556 2487 1487 1119 1038 585
48
PshAI GACnn'nnGTC
Cuts at: 1613 2964 8268 14790 1613
Size: 1351 5304 6522 5466
Fragments arranged by size:
6522 5466 5304 1351
PsiI TTA'TAA
Cuts at: 10982 13580 16213 10982
Size: 2598 2633 13412
Fragments arranged by size:
13412 2633 2598
PspOMI G'GGCC_C
Cuts at: 8675 10222 8675
Size: 1547 17096
PspXI vC'TCGA_Gb
Cuts at: 8735 13620 8735
Size: 4885 13758
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 8051 8083 8051
Size: 32 18611
PstI C_TGCA'G
Cuts at: 830 2097 3384 3550 8892 9246 12073 12255 13339
Size: 1267 1287 166 5342 354 2827 182 1084
Cuts at: 13339 13430 14227 14355 15474 15995 830
Size: 91 797 128 1119 521 3478
Fragments arranged by size:
5342 3478 2827 1287 1267 1119 1084 797
521 354 182 166 128 91
PvuI CG_AT'CG
Cuts at: 2001 14002 2001
Size: 12001 6642
PvuII CAG'CTG
Cuts at: 3826 3976 6112 6570 9331 9460 11442 11794 12404
Size: 150 2136 458 2761 129 1982 352 610
Cuts at: 12404 12536 13237 14329 15469 15491 15603 15772 3826
Size: 132 701 1092 1140 22 112 169 6697
Fragments arranged by size:
6697 2761 2136 1982 1140 1092 701 610
458 352 169 150 132 129 112 22
SacI G_AGCT'C
Cuts at: 5571 11154 15621 5571
Size: 5583 4467 8593
Fragments arranged by size:
8593 5583 4467
SalI G'TCGA_C
Cuts at: 1936 11639 14867 15985 1936
Size: 9703 3228 1118 4594
Fragments arranged by size:
9703 4594 3228 1118
SanDI GG'GwC_CC
Cuts at: 10029 10029
Size: 18643
SapI GCTCTTCn'nnn_
Cuts at: 2174 5946 8480 8687 8846 9383 12437 12441 2174
Size: 3772 2534 207 159 537 3054 4 8376
Fragments arranged by size:
8376 3772 3054 2534 537 207 159 4
SbfI CC_TGCA'GG
Cuts at: 15995 15995
Size: 18643
ScaI AGT'ACT
Cuts at: 4171 13152 13484 16891 18603 4171
Size: 8981 332 3407 1712 4211
Fragments arranged by size:
8981 4211 3407 1712 332
SexAI A'CCwGG_T
Cuts at: 5016 11643 11913 12596 5016
Size: 6627 270 683 11063
Fragments arranged by size:
11063 6627 683 270
SfcI C'TryA_G
Cuts at: 826 1045 2093 3380 3546 8888 9242 12069 12251
Size: 219 1048 1287 166 5342 354 2827 182
Cuts at: 12251 12611 13335 13426 14223 14351 15470 15991 17126
Size: 360 724 91 797 128 1119 521 1135
Cuts at: 17126 17807 17998 826
Size: 681 191 1471
Fragments arranged by size:
5342 2827 1471 1287 1135 1119 1048 797
724 681 521 360 354 219 191 182
166 128 91
SfoI GGC'GCC
Cuts at: 2148 15122 15505 15655 2148
Size: 12974 383 150 5136
Fragments arranged by size:
12974 5136 383 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18643
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 18643
SmaI CCC'GGG
Cuts at: 1872 2017 10221 13049 13088 15970 1872
Size: 145 8204 2828 39 2882 4545
Fragments arranged by size:
8204 4545 2882 2828 145 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3166 4202 8735 10791 11228 11668
Size: 197 926 1223 1036 4533 2056 437 440
Cuts at: 11668 13457 13620 13729 15197 16747 17648 17910 820
Size: 1789 163 109 1468 1550 901 262 1553
Fragments arranged by size:
4533 2056 1789 1553 1550 1468 1223 1036
926 901 440 437 262 197 163 109
SnaBI TAC'GTA
Cuts at: 493 16434 493
Size: 15941 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 18643
SphI G_CATG'C
Cuts at: 1132 3252 13630 14363 14862 16001 1132
Size: 2120 10378 733 499 1139 3774
Fragments arranged by size:
10378 3774 2120 1139 733 499
SspI AAT'ATT
Cuts at: 5 52 2602 4734 10779 16567 5
Size: 47 2550 2132 6045 5788 2081
Fragments arranged by size:
6045 5788 2550 2132 2081 47
StuI AGG'CCT
Cuts at: 1517 3787 9398 10464 11127 1517
Size: 2270 5611 1066 663 9033
Fragments arranged by size:
9033 5611 2270 1066 663
StyI C'CwwG_G
Cuts at: 513 1065 4009 4593 5904 6295 7689 8772 9060
Size: 552 2944 584 1311 391 1394 1083 288
Cuts at: 9060 9306 9654 10320 11931 12273 13446 14013 14041
Size: 246 348 666 1611 342 1173 567 28
Cuts at: 14041 14385 14400 14685 15507 16082 513
Size: 344 15 285 822 575 3074
Fragments arranged by size:
3074 2944 1611 1394 1311 1173 1083 822
666 584 575 567 552 391 348 344
342 288 285 246 28 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5012 6890 7613 7847 8258 8660 9028
Size: 1255 3432 1878 723 234 411 402 368
Cuts at: 9028 9536 10093 10373 10399 13963 14272 14917 14963
Size: 508 557 280 26 3564 309 645 46
Cuts at: 14963 15427 15527 16670 16687 16840 17025 17470 325
Size: 464 100 1143 17 153 185 445 1498
Fragments arranged by size:
3564 3432 1878 1498 1255 1143 723 645
557 508 464 445 411 402 368 309
280 234 185 153 100 46 26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 4501 4981
Size: 274 80 33 51 1443 2192 332 480
Cuts at: 4981 5026 6064 6349 6793 8166 9747 9924 10620
Size: 45 1038 285 444 1373 1581 177 696
Cuts at: 10620 10902 13150 13482 14541 16889 18601 96
Size: 282 2248 332 1059 2348 1712 138
Fragments arranged by size:
2348 2248 2192 1712 1581 1443 1373 1059
1038 696 480 444 332 332 285 282
274 177 138 80 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2040 2072 3103 3135 4667 4699 4813 4845 8163
Size: 32 1031 32 1532 32 114 32 3318
Cuts at: 8163 8195 8918 8950 10167 10199 13036 13068 18603
Size: 32 723 32 1217 32 2837 32 5535
Cuts at: 18603 18635 2040
Size: 32 2048
Fragments arranged by size:
5535 3318 2837 2048 1532 1217 1031 723
114 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3314 7388 9725 13370 13393 1316
Size: 1998 4074 2337 3645 23 6566
Fragments arranged by size:
6566 4074 3645 2337 1998 23
XbaI T'CTAG_A
Cuts at: 15979 15979
Size: 18643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4411 5911 8654 14756 15010 15583 4411
Size: 1500 2743 6102 254 573 7471
Fragments arranged by size:
7471 6102 2743 1500 573 254
XhoI C'TCGA_G
Cuts at: 1943 8735 13620 1943
Size: 6792 4885 6966
Fragments arranged by size:
6966 6792 4885
XmaI C'CCGG_G
Cuts at: 1870 2015 10219 13047 13086 15968 1870
Size: 145 8204 2828 39 2882 4545
Fragments arranged by size:
8204 4545 2882 2828 145 39
XmnI GAAnn'nnTTC
Cuts at: 3017 3261 6119 6962 8108 8622 10574 12587 12749
Size: 244 2858 843 1146 514 1952 2013 162
Cuts at: 12749 16772 3017
Size: 4023 4888
Fragments arranged by size:
4888 4023 2858 2013 1952 1146 843 514
244 162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2266 16451 276
Size: 53 83 186 936 732 14185 2468
Fragments arranged by size:
14185 2468 936 732 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I FseI FspAI PacI
RsrII SacII SfiI SrfI SwaI