Restriction map for
pFN21AA0322
(Circular) (Six-base) MAPSORT of: pFN21AA0322.seq Check: 1242 from: 1 to: 9523
pFN21AA0322 9523 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4955 4955
Size: 9523
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7333 278
Size: 53 83 186 936 5797 2468
Fragments arranged by size:
5797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6405 6866 1937
Size: 4468 461 4594
Fragments arranged by size:
4594 4468 461
Acc65I G'GTAC_C
Cuts at: 4249 6844 4249
Size: 2595 6928
AclI AA'CG_TT
Cuts at: 1563 7650 8023 1563
Size: 6087 373 3063
Fragments arranged by size:
6087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 3330 5147 7586 8970 9238 1632
Size: 1079 619 1817 2439 1384 268 1917
Fragments arranged by size:
2439 1917 1817 1384 1079 619 268
AfeI AGC'GCT
Cuts at: 2891 5230 6566 2891
Size: 2339 1336 5848
Fragments arranged by size:
5848 2339 1336
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9326
AflIII A'CryG_T
Cuts at: 1203 4026 5037 8422 9399 1203
Size: 2823 1011 3385 977 1327
Fragments arranged by size:
3385 2823 1327 1011 977
AgeI A'CCGG_T
Cuts at: 8354 8354
Size: 9523
AhdI GACnn_n'nnGTC
Cuts at: 4285 8252 4285
Size: 3967 5556
AleI CACnn'nnGTG
Cuts at: 1166 2834 6293 1166
Size: 1668 3459 4396
Fragments arranged by size:
4396 3459 1668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6739 1184
Size: 5555 3968
AlwNI CAG_nnn'CTG
Cuts at: 2290 3606 6586 7266 8838 2290
Size: 1316 2980 680 1572 2975
Fragments arranged by size:
2980 2975 1572 1316 680
ApaI G_GGCC'C
Cuts at: 2512 2512
Size: 9523
ApaLI G'TGCA_C
Cuts at: 7580 8736 7580
Size: 1156 8367
ApoI r'AATT_y
Cuts at: 1493 3093 5026 6161 6832 7058 7210 9319 1493
Size: 1600 1933 1135 671 226 152 2109 1697
Fragments arranged by size:
2109 1933 1697 1600 1135 671 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8077 160
Size: 872 7045 1606
Fragments arranged by size:
7045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9523
AvaI C'yCGr_G
Cuts at: 1870 1943 3662 3863 4886 5067 6409 6848 1870
Size: 73 1719 201 1023 181 1342 439 4545
Fragments arranged by size:
4545 1719 1342 1023 439 201 181 73
BamHI G'GATC_C
Cuts at: 6853 7220 6853
Size: 367 9156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3154 4249 6461 6844 8299
Size: 325 217 627 1367 1095 2212 383 1455
Cuts at: 8299 618
Size: 1842
Fragments arranged by size:
2212 1842 1455 1367 1095 627 383 325
217
BanII G_rGCy'C
Cuts at: 1391 2512 3327 3462 3804 3864 4271 5662 5829
Size: 1121 815 135 342 60 407 1391 167
Cuts at: 5829 5925 6842 1391
Size: 96 917 4072
Fragments arranged by size:
4072 1391 1121 917 815 407 342 167
135 96 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3432 4192 4924 5168 928
Size: 971 1533 760 732 244 5283
Fragments arranged by size:
5283 1533 971 760 732 244
BbvCI CC'TCA_GC
Cuts at: 3252 3252
Size: 9523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2088 2122 4123 4157 7714
Size: 34 50 34 67 34 2001 34 3557
Cuts at: 7714 7748 1903
Size: 34 3678
Fragments arranged by size:
3678 3557 2001 67 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6762 7415 8625 6762
Size: 653 1210 7660
Fragments arranged by size:
7660 1210 653
BclI T'GATC_A
Cuts at: 1548 2616 1548
Size: 1068 8455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 8134 136
Size: 107 122 71 631 605 397 6065 1525
Fragments arranged by size:
6065 1525 631 605 397 122 107 71
BglII A'GATC_T
Cuts at: 2475 4182 4795 9518 2475
Size: 1707 613 4723 2480
Fragments arranged by size:
4723 2480 1707 613
BlpI GC'TnA_GC
Cuts at: 3803 5419 6940 3803
Size: 1616 1521 6386
Fragments arranged by size:
6386 1616 1521
Bme1580I G_kGCm'C
Cuts at: 1790 2280 2512 4341 6627 6718 7584 8740 1790
Size: 490 232 1829 2286 91 866 1156 2573
Fragments arranged by size:
2573 2286 1829 1156 866 490 232 91
BmgBI CAC'GTC
Cuts at: 1318 2836 4074 1318
Size: 1518 1238 6767
Fragments arranged by size:
6767 1518 1238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2311 2523 3357
Size: 416 53 83 399 24 894 212 834
Cuts at: 3357 4516 4777 7254 8212 442
Size: 1159 261 2477 958 1753
Fragments arranged by size:
2477 1753 1159 958 894 834 416 399
261 212 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3997 3997
Size: 9523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2951 3569 3650 3861 3967 4806 5192 5253
Size: 1712 618 81 211 106 839 386 61
Cuts at: 5253 5813 6297 6464 8183 1239
Size: 560 484 167 1719 2579
Fragments arranged by size:
2579 1719 1712 839 618 560 484 386
211 167 106 81 61
Bpu10I CC'TnA_GC
Cuts at: 3252 4272 3252
Size: 1020 8503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3761 5504 6038 6839 7648 8513 8811 3761
Size: 1743 534 801 809 865 298 4473
Fragments arranged by size:
4473 1743 865 809 801 534 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2579 3276 3301 3659 8186 882
Size: 617 1080 697 25 358 4527 2219
Fragments arranged by size:
4527 2219 1080 697 617 358 25
BsaAI yAC'GTr
Cuts at: 493 1204 4342 4555 7314 493
Size: 711 3138 213 2759 2702
Fragments arranged by size:
3138 2759 2702 711 213
BsaBI GATnn'nnATC
Cuts at: 5505 6528 9517 5505
Size: 1023 2989 5511
Fragments arranged by size:
5511 2989 1023
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 2101 2184 7330
Size: 53 83 186 936 539 29 83 5146
Cuts at: 7330 7712 8347 275
Size: 382 635 1451
Fragments arranged by size:
5146 1451 936 635 539 382 186 83
83 53 29
BsaWI w'CCGG_w
Cuts at: 7956 8354 8628 8775 9186 7956
Size: 398 274 147 411 8293
Fragments arranged by size:
8293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2222 2252 3752 3782 4103
Size: 30 165 30 819 30 1500 30 321
Cuts at: 4103 4133 4387 4417 5190 5220 6257 6287 1178
Size: 30 254 30 773 30 1037 30 4414
Fragments arranged by size:
4414 1500 1037 819 773 321 254 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2770 3071 3416 3470 3482 3524 3539 3542
Size: 1584 301 345 54 12 42 15 3
Cuts at: 3542 3758 3761 3775 3776 3779 4048 4153 4195
Size: 216 3 14 1 3 269 105 42
Cuts at: 4195 4235 4238 4241 4755 5277 5879 6294 1186
Size: 40 3 3 514 522 602 415 4415
Fragments arranged by size:
4415 1584 602 522 514 415 345 301
269 216 105 54 42 42 40 15
14 12 3 3 3 3 3 1
BseYI C'CCAG_C
Cuts at: 2327 3190 3317 3439 3917 3959 4301 4428 4732
Size: 863 127 122 478 42 342 127 304
Cuts at: 4732 4861 5073 8726 9222 2327
Size: 129 212 3653 496 2628
Fragments arranged by size:
3653 2628 863 496 478 342 304 212
129 127 127 122 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1997 3387 3689 5999 1163
Size: 573 261 1390 302 2310 4687
Fragments arranged by size:
4687 2310 1390 573 302 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 5173 6040 6973 7734 8762
Size: 275 186 1213 1959 867 933 761 1028
Cuts at: 8762 1540
Size: 2301
Fragments arranged by size:
2301 1959 1213 1028 933 867 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 3327 4196 5829 5925 7584 7669 8740 3327
Size: 869 1633 96 1659 85 1071 4110
Fragments arranged by size:
4110 1659 1633 1071 869 96 85
BsmI GAATG_Cn'
Cuts at: 7034 7127 7034
Size: 93 9430
BsmBI CGTCTCn'nnnn_
Cuts at: 2079 3626 2079
Size: 1547 7976
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2512 3327 3462 3804 3864 4196
Size: 399 490 232 815 135 342 60 332
Cuts at: 4196 4271 4341 5662 5829 5925 6627 6718 6842
Size: 75 70 1321 167 96 702 91 124
Cuts at: 6842 7584 7669 8740 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1321 1071 815 742 702 490 399
342 332 232 167 135 124 96 91
85 75 70 60
BspEI T'CCGG_A
Cuts at: 9186 9186
Size: 9523
BspHI T'CATG_A
Cuts at: 7412 9142 7412
Size: 1730 7793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4370 4955 6878 844
Size: 3526 585 1923 3489
Fragments arranged by size:
3526 3489 1923 585
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 2199 2238 7410 1394
Size: 429 296 80 39 5172 3507
Fragments arranged by size:
5172 3507 429 296 80 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 4677 5333 6664 8018 8192 65
Size: 1183 3429 656 1331 1354 174 1396
Fragments arranged by size:
3429 1396 1354 1331 1183 656 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 8167 8354 1700
Size: 110 293 6064 187 2869
Fragments arranged by size:
6064 2869 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9523
BssSI C'ACGA_G
Cuts at: 4045 4060 4090 4105 6091 7583 8595 4045
Size: 15 30 15 1986 1492 1012 4973
Fragments arranged by size:
4973 1986 1492 1012 30 15 15
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3606 3712 4077 4092 794
Size: 2812 106 365 15 6225
Fragments arranged by size:
6225 2812 365 106 15
BstBI TT'CG_AA
Cuts at: 5311 8327 5311
Size: 3016 6507
BstEII G'GTnAC_C
Cuts at: 1184 2221 6515 1184
Size: 1037 4294 4192
Fragments arranged by size:
4294 4192 1037
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 4506 6442 2248
Size: 2258 1936 5329
Fragments arranged by size:
5329 2258 1936
BstYI r'GATC_y
Cuts at: 1972 2475 2759 4182 4795 5506 6739 6853 7220
Size: 503 284 1423 613 711 1233 114 367
Cuts at: 7220 7605 7622 9074 9160 9172 9518 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1423 1233 711 613 503 385
367 346 284 114 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2162 4454 2162
Size: 2292 7231
BtgI C'CryG_G
Cuts at: 513 1065 2067 2395 2494 2646 2743 3671 4167
Size: 552 1002 328 99 152 97 928 496
Cuts at: 4167 4307 4374 4528 6465 6649 513
Size: 140 67 154 1937 184 3387
Fragments arranged by size:
3387 1937 1002 928 552 496 328 184
154 152 140 99 97 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8784
Fragments arranged by size:
8784 683 56
BtsI GCAGTG_nn'
Cuts at: 3139 3208 3413 6185 7039 7833 7853 3139
Size: 69 205 2772 854 794 20 4809
Fragments arranged by size:
4809 2772 854 794 205 69 20
ClaI AT'CG_AT
Cuts at: 1554 2439 4485 1554
Size: 885 2046 6592
Fragments arranged by size:
6592 2046 885
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5125 5160 529
Size: 35 4561 35 4892
Fragments arranged by size:
4892 4561 35 35
DraI TTT'AAA
Cuts at: 2566 6827 2566
Size: 4261 5262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2604 8530 809
Size: 507 1288 5926 1802
Fragments arranged by size:
5926 1802 1288 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2974 3378
Size: 54 1425 50 275 18 231 913 404
Cuts at: 3378 3822 3895 3924 4323 5277 6970 7859 9488
Size: 444 73 29 399 954 1693 889 1629
Cuts at: 9488 8
Size: 43
Fragments arranged by size:
1693 1629 1425 954 913 889 444 404
399 275 231 73 54 50 43 29
18
EagI C'GGCC_G
Cuts at: 1537 1812 6970 1537
Size: 275 5158 4090
Fragments arranged by size:
5158 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3366 3501 3584 3840 3852 4500 5166 5532
Size: 2013 135 83 256 12 648 666 366
Cuts at: 5532 5671 5930 6231 7453 1353
Size: 139 259 301 1222 3423
Fragments arranged by size:
3423 2013 1222 666 648 366 301 259
256 139 135 83 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3709 4761 6505 8110 8484 8630
Size: 1064 138 2120 1052 1744 1605 374 146
Cuts at: 8630 387
Size: 1280
Fragments arranged by size:
2120 1744 1605 1280 1064 1052 374 146
138
EcoICRI GAG'CTC
Cuts at: 3325 5827 5923 3325
Size: 2502 96 6925
Fragments arranged by size:
6925 2502 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2711 2951 3330 3569 3650 3861 3967
Size: 393 1079 240 379 239 81 211 106
Cuts at: 3967 4806 5147 5192 5253 5813 6297 6464 7586
Size: 839 341 45 61 560 484 167 1122
Cuts at: 7586 8183 8970 9238 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1122 1079 839 787 597 560 484
393 379 341 268 240 239 211 167
106 81 61 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3332 3398 3551 3845 3332
Size: 66 153 294 9010
Fragments arranged by size:
9010 294 153 66
EcoO109I rG'GnC_Cy
Cuts at: 2287 2508 2509 3305 3603 3658 5869 6096 2287
Size: 221 1 796 298 55 2211 227 5714
Fragments arranged by size:
5714 2211 796 298 227 221 55 1
EcoRI G'AATT_C
Cuts at: 6161 6832 7210 6161
Size: 671 378 8474
Fragments arranged by size:
8474 671 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2497 2911 5679 2497
Size: 414 2768 6341
Fragments arranged by size:
6341 2768 414
FspI TGC'GCA
Cuts at: 8029 8029
Size: 9523
HaeII r_GCGC'y
Cuts at: 1678 2893 4104 4179 4302 5232 6380 6568 6673
Size: 1215 1211 75 123 930 1148 188 105
Cuts at: 6673 8670 1678
Size: 1997 2531
Fragments arranged by size:
2531 1997 1215 1211 1148 930 188 123
105 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 2351 2383 2432
Size: 32 135 32 650 32 97 32 49
Cuts at: 2432 2464 2744 2751 2776 2783 3012 3044 3068
Size: 32 280 7 25 7 229 32 24
Cuts at: 3068 3100 3392 3424 3752 3784 4271 4303 5190
Size: 32 292 32 328 32 487 32 887
Cuts at: 5190 5222 5477 5509 5531 5563 6257 6289 8164
Size: 32 255 32 22 32 694 32 1875
Cuts at: 8164 8196 8238 8270 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1875 887 694 650 487 328 292
280 255 229 135 97 49 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 25
24 22 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 6786 6867 7113 669
Size: 988 281 4848 81 246 3079
Fragments arranged by size:
4848 3079 988 281 246 81
HindIII A'AGCT_T
Cuts at: 748 5416 748
Size: 4668 4855
HpaI GTT'AAC
Cuts at: 6786 7113 6786
Size: 327 9196
KpnI G_GTAC'C
Cuts at: 4253 6848 4253
Size: 2595 6928
MfeI C'AATT_G
Cuts at: 7122 7122
Size: 9523
MluI A'CGCG_T
Cuts at: 4026 9399 4026
Size: 5373 4150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2588 3011 4607 4999 5763 5839 8637 8821
Size: 1283 423 1596 392 764 76 2798 184
Cuts at: 8821 1305
Size: 2007
Fragments arranged by size:
2798 2007 1596 1283 764 423 392 184
76
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3897 3926 5279 10
Size: 54 1425 343 2065 29 1353 4254
Fragments arranged by size:
4254 2065 1425 1353 343 54 29
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2834 3000 3048 5036 5325 6293
Size: 648 1080 588 166 48 1988 289 968
Cuts at: 6293 6721 7481 7840 7999 518
Size: 428 760 359 159 2042
Fragments arranged by size:
2042 1988 1080 968 760 648 588 428
359 289 166 159 48
MspA1I CmG'CkG
Cuts at: 1606 3010 3190 3251 3388 3574 3673 3827 4865
Size: 1404 180 61 137 186 99 154 1038
Cuts at: 4865 5601 6467 6543 6939 7616 8764 9009 1606
Size: 736 866 76 396 677 1148 245 2120
Fragments arranged by size:
2120 1404 1148 1038 866 736 677 396
245 186 180 154 137 99 76 61
NcoI C'CATG_G
Cuts at: 513 1065 2067 2494 2646 2743 513
Size: 552 1002 427 152 97 7293
Fragments arranged by size:
7293 1002 552 427 152 97
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9523
NotI GC'GGCC_GC
Cuts at: 6970 6970
Size: 9523
NspI r_CATG'y
Cuts at: 1132 1229 2347 3715 4612 4910 5041 6726 6881
Size: 97 1118 1368 897 298 131 1685 155
Cuts at: 6881 8426 1132
Size: 1545 2229
Fragments arranged by size:
2229 1685 1545 1368 1118 897 298 155
131 97
PasI CC'CwG_GG
Cuts at: 1348 3724 5579 6711 1348
Size: 2376 1855 1132 4160
Fragments arranged by size:
4160 2376 1855 1132
PciI A'CATG_T
Cuts at: 5037 8422 5037
Size: 3385 6138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2155 2647 3292 4636 1108
Size: 1047 492 645 1344 5995
Fragments arranged by size:
5995 1344 1047 645 492
PfoI T'CCnGG_A
Cuts at: 2928 2928
Size: 9523
PmeI GTTT'AAAC
Cuts at: 6827 6827
Size: 9523
PmlI CAC'GTG
Cuts at: 4342 4555 4342
Size: 213 9310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7592 7624 9105 9137 7592
Size: 32 1481 32 7978
Fragments arranged by size:
7978 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 3305 3658 2287
Size: 1018 353 8152
Fragments arranged by size:
8152 1018 353
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9523
PsiI TTA'TAA
Cuts at: 7093 7093
Size: 9523
PspOMI G'GGCC_C
Cuts at: 2508 2508
Size: 9523
PspXI vC'TCGA_Gb
Cuts at: 6409 6409
Size: 9523
PstI C_TGCA'G
Cuts at: 830 3573 4040 4857 5565 6600 6875 830
Size: 2743 467 817 708 1035 275 3478
Fragments arranged by size:
3478 2743 1035 817 708 467 275
PvuI CG_AT'CG
Cuts at: 2001 3214 2001
Size: 1213 8310
PvuII CAG'CTG
Cuts at: 3010 3190 3251 3388 3574 3010
Size: 180 61 137 186 8959
Fragments arranged by size:
8959 186 180 137 61
SacI G_AGCT'C
Cuts at: 3327 5829 5925 3327
Size: 2502 96 6925
Fragments arranged by size:
6925 2502 96
SacII CC_GC'GG
Cuts at: 3674 6468 3674
Size: 2794 6729
SalI G'TCGA_C
Cuts at: 1936 6865 1936
Size: 4929 4594
SanDI GG'GwC_CC
Cuts at: 3658 3658
Size: 9523
SapI GCTCTTCn'nnn_
Cuts at: 3366 3852 5930 3366
Size: 486 2078 6959
Fragments arranged by size:
6959 2078 486
SbfI CC_TGCA'GG
Cuts at: 5565 6875 5565
Size: 1310 8213
ScaI AGT'ACT
Cuts at: 5954 7771 9483 5954
Size: 1817 1712 5994
Fragments arranged by size:
5994 1817 1712
SexAI A'CCwGG_T
Cuts at: 2217 2289 2217
Size: 72 9451
SfcI C'TryA_G
Cuts at: 826 1045 3569 4036 4051 4066 4081 4096 4111
Size: 219 2524 467 15 15 15 15 15
Cuts at: 4111 4853 5561 5707 5878 6596 6871 8006 8687
Size: 742 708 146 171 718 275 1135 681
Cuts at: 8687 8878 826
Size: 191 1471
Fragments arranged by size:
2524 1471 1135 742 718 708 681 467
275 219 191 171 146 15 15 15
15 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9523
SgrAI Cr'CCGG_yG
Cuts at: 1700 2103 1700
Size: 403 9120
SmaI CCC'GGG
Cuts at: 1872 5069 6850 1872
Size: 3197 1781 4545
Fragments arranged by size:
4545 3197 1781
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3776 5483 6017 6409 6818 7627
Size: 197 926 1833 1707 534 392 409 809
Cuts at: 7627 8528 8790 820
Size: 901 262 1553
Fragments arranged by size:
1833 1707 1553 926 901 809 534 409
392 262 197
SnaBI TAC'GTA
Cuts at: 493 7314 493
Size: 6821 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9523
SphI G_CATG'C
Cuts at: 1132 3715 4612 6881 1132
Size: 2583 897 2269 3774
Fragments arranged by size:
3774 2583 2269 897
SspI AAT'ATT
Cuts at: 5 52 6149 7447 5
Size: 47 6097 1298 2081
Fragments arranged by size:
6097 2081 1298 47
StuI AGG'CCT
Cuts at: 1517 2058 4459 5240 1517
Size: 541 2401 781 5800
Fragments arranged by size:
5800 2401 781 541
StyI C'CwwG_G
Cuts at: 513 1065 2067 2494 2593 2646 2743 3450 3462
Size: 552 1002 427 99 53 97 707 12
Cuts at: 3462 6962 513
Size: 3500 3074
Fragments arranged by size:
3500 3074 1002 707 552 427 99 97
53 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3060 3135 3182 3386 6386 7550 7567
Size: 1255 1480 75 47 204 3000 1164 17
Cuts at: 7567 7720 7905 8350 325
Size: 153 185 445 1498
Fragments arranged by size:
3000 1498 1480 1255 1164 445 204 185
153 75 47 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5952 6318 7769
Size: 274 80 33 51 1443 3975 366 1451
Cuts at: 7769 9481 96
Size: 1712 138
Fragments arranged by size:
3975 1712 1451 1443 366 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3669 3701 4387 4419 9483 9515 3669
Size: 32 686 32 5064 32 3677
Fragments arranged by size:
5064 3677 686 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2219 2291 1316
Size: 903 72 8548
Fragments arranged by size:
8548 903 72
XbaI T'CTAG_A
Cuts at: 2081 5252 6859 2081
Size: 3171 1607 4745
Fragments arranged by size:
4745 3171 1607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 2836 3491 4633 2501
Size: 335 655 1142 7391
Fragments arranged by size:
7391 1142 655 335
XhoI C'TCGA_G
Cuts at: 1943 6409 1943
Size: 4466 5057
XmaI C'CCGG_G
Cuts at: 1870 5067 6848 1870
Size: 3197 1781 4545
Fragments arranged by size:
4545 3197 1781
XmnI GAAnn'nnTTC
Cuts at: 2376 7652 2376
Size: 5276 4247
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7331 276
Size: 53 83 186 936 5797 2468
Fragments arranged by size:
5797 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PsrI RsrII SfiI SfoI SrfI
SwaI