Restriction map for
pFN21AA0302
(Circular) (Six-base) MAPSORT of: pFN21AA0302.seq Check: 5379 from: 1 to: 11875
pFN21AA0302 11875 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7567 7567
Size: 11875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9685 278
Size: 53 83 186 936 8149 2468
Fragments arranged by size:
8149 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3153 8139 8600 9218 1937
Size: 1216 4986 461 618 4594
Fragments arranged by size:
4986 4594 1216 618 461
Acc65I G'GTAC_C
Cuts at: 9196 9196
Size: 11875
AclI AA'CG_TT
Cuts at: 1563 10002 10375 1563
Size: 8439 373 3063
Fragments arranged by size:
8439 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 4115 4781 5295 5745 5894 7061 7664
Size: 1076 1407 666 514 450 149 1167 603
Cuts at: 7664 8015 9036 9136 9938 11322 11590 1632
Size: 351 1021 100 802 1384 268 1917
Fragments arranged by size:
1917 1407 1384 1167 1076 1021 802 666
603 514 450 351 268 149 100
AfeI AGC'GCT
Cuts at: 3488 7418 8119 3488
Size: 3930 701 7244
Fragments arranged by size:
7244 3930 701
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11678
AflIII A'CryG_T
Cuts at: 1203 5913 8739 8884 10774 11751 1203
Size: 4710 2826 145 1890 977 1327
Fragments arranged by size:
4710 2826 1890 1327 977 145
AgeI A'CCGG_T
Cuts at: 4025 10706 4025
Size: 6681 5194
AhdI GACnn_n'nnGTC
Cuts at: 10604 10604
Size: 11875
AleI CACnn'nnGTG
Cuts at: 1166 3228 4733 1166
Size: 2062 1505 8308
Fragments arranged by size:
8308 2062 1505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6578 7812 1184
Size: 5394 1234 5247
Fragments arranged by size:
5394 5247 1234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6395 6427 7116 7148 6395
Size: 32 689 32 11122
Fragments arranged by size:
11122 689 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3622 3679 4231 4759 5443 6096 6286 6601 6901
Size: 57 552 528 684 653 190 315 300
Cuts at: 6901 7393 7711 8360 8458 8730 9618 11190 3622
Size: 492 318 649 98 272 888 1572 4307
Fragments arranged by size:
4307 1572 888 684 653 649 552 528
492 318 315 300 272 190 98 57
ApaI G_GGCC'C
Cuts at: 3910 4092 4378 5356 7675 8538 8570 8848 3910
Size: 182 286 978 2319 863 32 278 6937
Fragments arranged by size:
6937 2319 978 863 286 278 182 32
ApaLI G'TGCA_C
Cuts at: 2396 4903 9932 11088 2396
Size: 2507 5029 1156 3183
Fragments arranged by size:
5029 3183 2507 1156
ApoI r'AATT_y
Cuts at: 1493 7208 9184 9410 9562 11671 1493
Size: 5715 1976 226 152 2109 1697
Fragments arranged by size:
5715 2109 1976 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10429 160
Size: 872 9397 1606
Fragments arranged by size:
9397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11875
AvaI C'yCGr_G
Cuts at: 1870 1943 2220 2288 2299 4322 4466 4521 5043
Size: 73 277 68 11 2023 144 55 522
Cuts at: 5043 5061 5400 6391 7224 7812 7878 8538 8562
Size: 18 339 991 833 588 66 660 24
Cuts at: 8562 8634 9042 9054 9200 1870
Size: 72 408 12 146 4545
Fragments arranged by size:
4545 2023 991 833 660 588 522 408
339 277 146 144 73 72 68 66
55 24 18 12 11
AvrII C'CTAG_G
Cuts at: 4963 4963
Size: 11875
BamHI G'GATC_C
Cuts at: 2340 2767 5685 7131 9205 9572 2340
Size: 427 2918 1446 2074 367 4643
Fragments arranged by size:
4643 2918 2074 1446 427 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2559 3687 4028 4483 6450
Size: 325 217 627 772 1128 341 455 1967
Cuts at: 6450 7986 9028 9196 10651 618
Size: 1536 1042 168 1455 1842
Fragments arranged by size:
1967 1842 1536 1455 1128 1042 772 627
455 341 325 217 168
BanII G_rGCy'C
Cuts at: 1391 2417 3255 3910 4092 4378 5356 5383 5697
Size: 1026 838 655 182 286 978 27 314
Cuts at: 5697 6033 6630 6775 7287 7675 8448 8538 8570
Size: 336 597 145 512 388 773 90 32
Cuts at: 8570 8848 8957 9194 1391
Size: 278 109 237 4072
Fragments arranged by size:
4072 1026 978 838 773 655 597 512
388 336 314 286 278 237 182 145
109 90 32 27
BbeI G_GCGC'C
Cuts at: 6454 7990 6454
Size: 1536 10339
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2520 2576 2781 4940 5816 8881 8988
Size: 971 621 56 205 2159 876 3065 107
Cuts at: 8988 928
Size: 3815
Fragments arranged by size:
3815 3065 2159 971 876 621 205 107
56
BbvCI CC'TCA_GC
Cuts at: 2279 3963 4000 4870 5556 7559 7629 9093 2279
Size: 1684 37 870 686 2003 70 1464 5061
Fragments arranged by size:
5061 2003 1684 1464 870 686 70 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4202 4236 4814 4848 5966 6000 6446
Size: 34 2265 34 578 34 1118 34 446
Cuts at: 6446 6480 10066 10100 1903
Size: 34 3586 34 3678
Fragments arranged by size:
3678 3586 2265 1118 578 446 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9767 10977 9767
Size: 1210 10665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11875
BfrBI ATG'CAT
Cuts at: 2723 2723
Size: 11875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2909 3752 4550
Size: 107 122 71 631 605 1237 843 798
Cuts at: 4550 5315 5981 6800 7319 10486 136
Size: 765 666 819 519 3167 1525
Fragments arranged by size:
3167 1525 1237 843 819 798 765 666
631 605 519 122 107 71
BglII A'GATC_T
Cuts at: 7953 11870 7953
Size: 3917 7958
BlpI GC'TnA_GC
Cuts at: 3326 4577 5567 6159 6696 8121 9292 3326
Size: 1251 990 592 537 1425 1171 5909
Fragments arranged by size:
5909 1425 1251 1171 990 592 537
Bme1580I G_kGCm'C
Cuts at: 1790 2400 3910 4033 4092 4225 4335 4378 4488
Size: 610 1510 123 59 133 110 43 110
Cuts at: 4488 4907 5356 6187 6874 7435 7675 8538 8570
Size: 419 449 831 687 561 240 863 32
Cuts at: 8570 8848 9033 9936 11092 1790
Size: 278 185 903 1156 2573
Fragments arranged by size:
2573 1510 1156 903 863 831 687 610
561 449 419 278 240 185 133 123
110 110 59 43 32
BmgBI CAC'GTC
Cuts at: 1318 2779 5914 1318
Size: 1461 3135 7279
Fragments arranged by size:
7279 3135 1461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2110 2756 4180
Size: 416 53 83 399 24 693 646 1424
Cuts at: 4180 5413 8409 9068 9606 10564 442
Size: 1233 2996 659 538 958 1753
Fragments arranged by size:
2996 1753 1424 1233 958 693 659 646
538 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 6406 7972 8971 2314
Size: 4092 1566 999 5218
Fragments arranged by size:
5218 4092 1566 999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2638 3230 3293 3389 3617 3935 4127
Size: 1133 266 592 63 96 228 318 192
Cuts at: 4127 4400 4514 4676 4685 4715 5048 5465 5600
Size: 273 114 162 9 30 333 417 135
Cuts at: 5600 5666 5720 6128 6209 6221 6776 6923 7074
Size: 66 54 408 81 12 555 147 151
Cuts at: 7074 7586 7638 8240 8313 9027 10535 1239
Size: 512 52 602 73 714 1508 2579
Fragments arranged by size:
2579 1508 1133 714 602 592 555 512
417 408 333 318 273 266 228 192
162 151 147 135 114 96 81 73
66 63 54 52 30 12 9
Bpu10I CC'TnA_GC
Cuts at: 2279 3014 3963 4000 4870 4877 5097 5556 7559
Size: 735 949 37 870 7 220 459 2003
Cuts at: 7559 7629 8515 9093 2279
Size: 70 886 578 5061
Fragments arranged by size:
5061 2003 949 886 870 735 578 459
220 70 37 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 4441 6059 6254 7385 8498 10000 10865 11163
Size: 2075 1618 195 1131 1113 1502 865 298
Cuts at: 11163 2366
Size: 3078
Fragments arranged by size:
3078 2075 1618 1502 1131 1113 865 298
195
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2145 3311 4011 4682 4904 6075 6104
Size: 617 646 1166 700 671 222 1171 29
Cuts at: 6104 10538 882
Size: 4434 2219
Fragments arranged by size:
4434 2219 1171 1166 700 671 646 617
222 29
BsaAI yAC'GTr
Cuts at: 493 1204 2229 9666 493
Size: 711 1025 7437 2702
Fragments arranged by size:
7437 2702 1025 711
BsaBI GATnn'nnATC
Cuts at: 7001 11869 7001
Size: 4868 7007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3459 4416 4837 5170
Size: 53 83 186 936 1926 957 421 333
Cuts at: 5170 5670 6451 7987 9682 10064 10699 275
Size: 500 781 1536 1695 382 635 1451
Fragments arranged by size:
1926 1695 1536 1451 957 936 781 635
500 421 382 333 186 83 53
BsaWI w'CCGG_w
Cuts at: 4025 7477 10308 10706 10980 11127 11538 4025
Size: 3452 2831 398 274 147 411 4362
Fragments arranged by size:
4362 3452 2831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2429 2516 2546 4274
Size: 30 165 30 996 30 87 30 1728
Cuts at: 4274 4304 4328 4358 6395 6425 7877 7907 1178
Size: 30 24 30 2037 30 1452 30 5146
Fragments arranged by size:
5146 2037 1728 1452 996 165 87 30
30 30 30 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 3575 3578 4229 4397 4400 4523 4625
Size: 1090 1299 3 651 168 3 123 102
Cuts at: 4625 5405 5483 5791 6419 7646 7885 7964 8216
Size: 780 78 308 628 1227 239 79 252
Cuts at: 8216 8255 8288 8291 8294 1186
Size: 39 33 3 3 4767
Fragments arranged by size:
4767 1299 1227 1090 780 651 628 308
252 239 168 123 102 79 78 39
33 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2079 4084 4224 4327 4979 5142 5185 5463 5473
Size: 2005 140 103 652 163 43 278 10
Cuts at: 5473 6096 6216 6566 6774 7168 7292 7674 8125
Size: 623 120 350 208 394 124 382 451
Cuts at: 8125 8382 8461 8896 9032 11078 11574 2079
Size: 257 79 435 136 2046 496 2380
Fragments arranged by size:
2380 2046 2005 652 623 496 451 435
394 382 350 278 257 208 163 140
136 124 120 103 79 43 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 3467 3704 3770 4188 4493 4694
Size: 573 462 1269 237 66 418 305 201
Cuts at: 4694 5153 6071 6206 6390 6690 7290 7409 7475
Size: 459 918 135 184 300 600 119 66
Cuts at: 7475 7601 8416 1163
Size: 126 815 4622
Fragments arranged by size:
4622 1269 918 815 600 573 462 459
418 305 300 237 201 184 135 126
119 66 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2979 3298 3442 4081 7642 7947
Size: 275 186 978 319 144 639 3561 305
Cuts at: 7947 8139 9325 10086 11114 1540
Size: 192 1186 761 1028 2301
Fragments arranged by size:
3561 2301 1186 1028 978 761 639 319
305 275 192 186 144
BsiHKAI G_wGCw'C
Cuts at: 2298 2400 3225 3255 4907 5568 5663 6033 6664
Size: 102 825 30 1652 661 95 370 631
Cuts at: 6664 6981 6992 7083 8484 8957 9111 9936 10021
Size: 317 11 91 1401 473 154 825 85
Cuts at: 10021 11092 2298
Size: 1071 3081
Fragments arranged by size:
3081 1652 1401 1071 825 825 661 631
473 370 317 154 102 95 91 85
30 11
BsiWI C'GTAC_G
Cuts at: 7404 7404
Size: 11875
BsmI GAATG_Cn'
Cuts at: 2723 2725 5966 8980 9386 9479 2723
Size: 2 3241 3014 406 93 5119
Fragments arranged by size:
5119 3241 3014 406 93 2
BsmBI CGTCTCn'nnnn_
Cuts at: 3410 3410
Size: 11875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2400 2417 3225 3255 3910 4033
Size: 399 508 102 17 808 30 655 123
Cuts at: 4033 4092 4225 4335 4378 4488 4907 5356 5383
Size: 59 133 110 43 110 419 449 27
Cuts at: 5383 5568 5663 5697 6033 6187 6630 6664 6775
Size: 185 95 34 336 154 443 34 111
Cuts at: 6775 6874 6981 6992 7083 7287 7435 7675 8448
Size: 99 107 11 91 204 148 240 773
Cuts at: 8448 8484 8538 8570 8848 8957 9033 9111 9194
Size: 36 54 32 278 109 76 78 83
Cuts at: 9194 9936 10021 11092 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 808 773 742 655 508 449
443 419 399 336 278 240 204 185
154 148 133 123 111 110 110 109
107 102 99 95 91 85 83 78
76 59 54 43 36 34 34 32
30 27 17 11
BspEI T'CCGG_A
Cuts at: 7477 11538 7477
Size: 4061 7814
BspHI T'CATG_A
Cuts at: 5655 6582 9764 11494 5655
Size: 927 3182 1730 6036
Fragments arranged by size:
6036 3182 1730 927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2283 2567 2676 3630 3717 5946 6093 6570
Size: 1439 284 109 954 87 2229 147 477
Cuts at: 6570 6671 7368 7567 7794 8586 9230 844
Size: 101 697 199 227 792 644 3489
Fragments arranged by size:
3489 2229 1439 954 792 697 644 477
284 227 199 147 109 101 87
BsrBI CCG'CTC
Cuts at: 1394 1823 3205 3515 3580 3847 4077 4195 4498
Size: 429 1382 310 65 267 230 118 303
Cuts at: 4498 5020 5096 5350 6523 6652 7060 8245 8284
Size: 522 76 254 1173 129 408 1185 39
Cuts at: 8284 9762 1394
Size: 1478 3507
Fragments arranged by size:
3507 1478 1382 1185 1173 522 429 408
310 303 267 254 230 129 118 76
65 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 4743 7287 8589 8982 10370 10544 65
Size: 1183 3495 2544 1302 393 1388 174 1396
Fragments arranged by size:
3495 2544 1396 1388 1302 1183 393 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2658 2994 3171 3660 4025 4080 4401
Size: 110 848 336 177 489 365 55 321
Cuts at: 4401 5009 5084 5138 5284 5370 5493 6447 7034
Size: 608 75 54 146 86 123 954 587
Cuts at: 7034 7596 7701 9022 10519 10706 1700
Size: 562 105 1321 1497 187 2869
Fragments arranged by size:
2869 1497 1321 954 848 608 587 562
489 365 336 321 187 177 146 123
110 105 86 75 55 54
BsrGI T'GTAC_A
Cuts at: 96 2247 2596 96
Size: 2151 349 9375
Fragments arranged by size:
9375 2151 349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11875
BssSI C'ACGA_G
Cuts at: 3224 3344 3722 5867 6728 6974 7163 8168 8956
Size: 120 378 2145 861 246 189 1005 788
Cuts at: 8956 9935 10947 3224
Size: 979 1012 4152
Fragments arranged by size:
4152 2145 1012 1005 979 861 788 378
246 189 120
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2395 5854 6283 6898 7711 8984 794
Size: 1601 3459 429 615 813 1273 3685
Fragments arranged by size:
3685 3459 1601 1273 813 615 429
BstBI TT'CG_AA
Cuts at: 4393 10679 4393
Size: 6286 5589
BstEII G'GTnAC_C
Cuts at: 1184 4136 5395 1184
Size: 2952 1259 7664
Fragments arranged by size:
7664 2952 1259
BstXI CCAn_nnnn'nTGG
Cuts at: 2764 6349 6544 7273 2764
Size: 3585 195 729 7366
Fragments arranged by size:
7366 3585 729 195
BstYI r'GATC_y
Cuts at: 1972 2340 2487 2767 5685 7131 7831 7953 9205
Size: 368 147 280 2918 1446 700 122 1252
Cuts at: 9205 9572 9957 9974 11426 11512 11524 11870 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2918 1977 1452 1446 1252 700 385 368
367 346 280 147 122 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5153 5298 6644 5153
Size: 145 1346 10384
Fragments arranged by size:
10384 1346 145
BtgI C'CryG_G
Cuts at: 513 1065 2865 2904 3060 3462 4731 5518 5664
Size: 552 1800 39 156 402 1269 787 146
Cuts at: 5664 5689 6399 6531 6543 7317 7398 7656 7827
Size: 25 710 132 12 774 81 258 171
Cuts at: 7827 8387 8815 513
Size: 560 428 3573
Fragments arranged by size:
3573 1800 1269 787 774 710 560 552
428 402 258 171 156 146 132 81
39 25 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3493 3727 5020 6504 6804 487
Size: 683 56 2267 234 1293 1484 300 5558
Fragments arranged by size:
5558 2267 1484 1293 683 300 234 56
BtsI GCAGTG_nn'
Cuts at: 2344 2369 2966 3459 4301 4628 5109 8196 9391
Size: 25 597 493 842 327 481 3087 1195
Cuts at: 9391 10185 10205 2344
Size: 794 20 4014
Fragments arranged by size:
4014 3087 1195 842 794 597 493 481
327 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2014 2049 4169 4204 529
Size: 35 1450 35 2120 35 8200
Fragments arranged by size:
8200 2120 1450 35 35 35
DraI TTT'AAA
Cuts at: 9179 9179
Size: 11875
DraIII CAC_nnn'GTG
Cuts at: 2199 3529 6898 8417 2199
Size: 1330 3369 1519 5657
Fragments arranged by size:
5657 3369 1519 1330
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4667 4790 10882 809
Size: 507 3351 123 6092 1802
Fragments arranged by size:
6092 3351 1802 507 123
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2660 2862 3003
Size: 54 1425 50 275 18 830 202 141
Cuts at: 3003 3282 3439 3477 3564 3852 4078 4128 4209
Size: 279 157 38 87 288 226 50 81
Cuts at: 4209 4403 5007 5086 5106 5286 5707 5973 6534
Size: 194 604 79 20 180 421 266 561
Cuts at: 6534 6915 6948 7275 7320 7395 7489 7591 7639
Size: 381 33 327 45 75 94 102 48
Cuts at: 7639 7659 7926 7944 8347 8390 8838 9322 10211
Size: 20 267 18 403 43 448 484 889
Cuts at: 10211 11840 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 830 604 561 484 448
421 403 381 327 288 279 275 267
266 226 202 194 180 157 141 102
94 87 81 79 75 54 50 50
48 45 43 43 38 33 20 20
18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 4078 7639 7944 9322 1537
Size: 275 1627 639 3561 305 1378 4090
Fragments arranged by size:
4090 3561 1627 1378 639 305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3116 3642 5172 5432 8056 9012 9805 1353
Size: 1763 526 1530 260 2624 956 793 3423
Fragments arranged by size:
3423 2624 1763 1530 956 793 526 260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3233 3248 4025 6818 6995 7091
Size: 1064 138 1644 15 777 2793 177 96
Cuts at: 7091 8302 10462 10836 10982 387
Size: 1211 2160 374 146 1280
Fragments arranged by size:
2793 2160 1644 1280 1211 1064 777 374
177 146 138 96 15
EcoICRI GAG'CTC
Cuts at: 3253 6031 8955 3253
Size: 2778 2924 6173
Fragments arranged by size:
6173 2924 2778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2638 2708 3230 3293 3389 3617
Size: 393 740 266 70 522 63 96 228
Cuts at: 3617 3935 4115 4127 4400 4514 4676 4685 4715
Size: 318 180 12 273 114 162 9 30
Cuts at: 4715 4781 5048 5295 5465 5600 5666 5720 5745
Size: 66 267 247 170 135 66 54 25
Cuts at: 5745 5894 6128 6209 6221 6776 6923 7061 7074
Size: 149 234 81 12 555 147 138 13
Cuts at: 7074 7586 7638 7664 8015 8240 8313 9027 9036
Size: 512 52 26 351 225 73 714 9
Cuts at: 9036 9136 9938 10535 11322 11590 1239
Size: 100 802 597 787 268 1524
Fragments arranged by size:
1524 802 787 740 714 597 555 522
512 393 351 318 273 268 267 266
247 234 228 225 180 170 162 149
147 138 135 114 100 96 81 73
70 66 66 63 54 52 30 26
25 13 12 12 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3947 4109 4211 4382 5297 5387 5510 8514 3947
Size: 162 102 171 915 90 123 3004 7308
Fragments arranged by size:
7308 3004 915 171 162 123 102 90
EcoO109I rG'GnC_Cy
Cuts at: 2083 2242 2857 3619 3676 3706 4088 4089 4204
Size: 159 615 762 57 30 382 1 115
Cuts at: 4204 4375 4590 4660 4960 5023 5149 5308 5449
Size: 171 215 70 300 63 126 159 141
Cuts at: 5449 6244 6417 6559 7513 7555 8335 8507 8535
Size: 795 173 142 954 42 780 172 28
Cuts at: 8535 8566 8727 8844 9098 2083
Size: 31 161 117 254 4860
Fragments arranged by size:
4860 954 795 780 762 615 382 300
254 215 173 172 171 161 159 159
142 141 126 117 115 70 63 57
42 31 30 28 1
EcoRI G'AATT_C
Cuts at: 9184 9562 9184
Size: 378 11497
EcoRV GAT'ATC
Cuts at: 6999 6999
Size: 11875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 7753 8917 2206
Size: 5547 1164 5164
Fragments arranged by size:
5547 5164 1164
FseI GG_CCGG'CC
Cuts at: 5288 5288
Size: 11875
FspI TGC'GCA
Cuts at: 3245 5219 8114 10381 3245
Size: 1974 2895 2267 4739
Fragments arranged by size:
4739 2895 2267 1974
HaeII r_GCGC'y
Cuts at: 1678 2920 3343 3490 3780 4565 6097 6454 7072
Size: 1242 423 147 290 785 1532 357 618
Cuts at: 7072 7266 7420 7525 7990 8121 11022 1678
Size: 194 154 105 465 131 2901 2531
Fragments arranged by size:
2901 2531 1532 1242 785 618 465 423
357 290 194 154 147 131 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2472 2504 2516
Size: 32 135 32 530 32 338 32 12
Cuts at: 2516 2548 2666 2698 2789 2821 4328 4360 7808
Size: 32 118 32 91 32 1507 32 3448
Cuts at: 7808 7840 7877 7909 7952 7984 8545 8577 10516
Size: 32 37 32 43 32 561 32 1939
Cuts at: 10516 10548 10590 10622 1373
Size: 32 42 32 2626
Fragments arranged by size:
3448 2626 1939 1507 561 530 338 135
118 91 43 42 37 32 32 32
32 32 32 32 32 32 32 32
32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4297 4795 6259 7783 8140 9219
Size: 988 281 2359 498 1464 1524 357 1079
Cuts at: 9219 9465 669
Size: 246 3079
Fragments arranged by size:
3079 2359 1524 1464 1079 988 498 357
281 246
HindIII A'AGCT_T
Cuts at: 748 3128 8225 748
Size: 2380 5097 4398
Fragments arranged by size:
5097 4398 2380
HpaI GTT'AAC
Cuts at: 9465 9465
Size: 11875
KasI G'GCGC_C
Cuts at: 6450 7986 6450
Size: 1536 10339
KpnI G_GTAC'C
Cuts at: 9200 9200
Size: 11875
MfeI C'AATT_G
Cuts at: 9474 9474
Size: 11875
MluI A'CGCG_T
Cuts at: 11751 11751
Size: 11875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3822 6048 10989 11173 1305
Size: 2517 2226 4941 184 2007
Fragments arranged by size:
4941 2517 2226 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3005 3854 5108 6536 7277
Size: 54 1425 343 1173 849 1254 1428 741
Cuts at: 7277 7928 8840 10
Size: 651 912 3045
Fragments arranged by size:
3045 1428 1425 1254 1173 912 849 741
651 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 3228 3746 4733 5600 6228 6489
Size: 648 1335 727 518 987 867 628 261
Cuts at: 6489 7907 9065 9833 10192 10351 518
Size: 1418 1158 768 359 159 2042
Fragments arranged by size:
2042 1418 1335 1158 987 867 768 727
648 628 518 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 2079 2616 3034 3280 3380 3438 3718 3835
Size: 473 537 418 246 100 58 280 117
Cuts at: 3835 3896 4172 4228 4733 5716 6220 6943 7400
Size: 61 276 56 505 983 504 723 457
Cuts at: 7400 7708 7829 7975 8000 8035 9291 9968 11116
Size: 308 121 146 25 35 1256 677 1148
Cuts at: 11116 11361 1606
Size: 245 2120
Fragments arranged by size:
2120 1256 1148 983 723 677 537 505
504 473 457 418 308 280 276 246
245 146 121 117 100 61 58 56
35 25
NaeI GCC'GGC
Cuts at: 3173 3662 4082 5011 5086 5286 5372 5495 6449
Size: 489 420 929 75 200 86 123 954
Cuts at: 6449 7036 7703 3173
Size: 587 667 7345
Fragments arranged by size:
7345 954 929 667 587 489 420 200
123 86 75
NarI GG'CG_CC
Cuts at: 6451 7987 6451
Size: 1536 10339
NcoI C'CATG_G
Cuts at: 513 1065 2904 5664 6531 6543 513
Size: 552 1839 2760 867 12 5845
Fragments arranged by size:
5845 2760 1839 867 552 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11875
NgoMIV G'CCGG_C
Cuts at: 3171 3660 4080 5009 5084 5284 5370 5493 6447
Size: 489 420 929 75 200 86 123 954
Cuts at: 6447 7034 7701 3171
Size: 587 667 7345
Fragments arranged by size:
7345 954 929 667 587 489 420 200
123 86 75
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11875
NotI GC'GGCC_GC
Cuts at: 9322 9322
Size: 11875
NruI TCG'CGA
Cuts at: 6468 6468
Size: 11875
NsiI A_TGCA'T
Cuts at: 2725 2725
Size: 11875
NspI r_CATG'y
Cuts at: 1132 1229 2338 5327 7190 7651 8888 9233 10778
Size: 97 1109 2989 1863 461 1237 345 1545
Cuts at: 10778 1132
Size: 2229
Fragments arranged by size:
2989 2229 1863 1545 1237 1109 461 345
97
PasI CC'CwG_GG
Cuts at: 1348 3803 4142 4957 5383 5452 8848 1348
Size: 2455 339 815 426 69 3396 4375
Fragments arranged by size:
4375 3396 2455 815 426 339 69
PciI A'CATG_T
Cuts at: 8884 10774 8884
Size: 1890 9985
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 2616 2905 4123 4231 6733 6901 6943
Size: 1242 266 289 1218 108 2502 168 42
Cuts at: 6943 7168 7444 1108
Size: 225 276 5539
Fragments arranged by size:
5539 2502 1242 1218 289 276 266 225
168 108 42
PfoI T'CCnGG_A
Cuts at: 2259 3615 5697 7134 7752 2259
Size: 1356 2082 1437 618 6382
Fragments arranged by size:
6382 2082 1437 1356 618
PmeI GTTT'AAAC
Cuts at: 9179 9179
Size: 11875
PmlI CAC'GTG
Cuts at: 2229 2229
Size: 11875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6395 6427 7749 7781 9944 9976 11457 11489 6395
Size: 32 1322 32 2163 32 1481 32 6781
Fragments arranged by size:
6781 2163 1481 1322 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2242 3619 3676 3706 4660 5023 5149 6244
Size: 159 1377 57 30 954 363 126 1095
Cuts at: 6244 6559 8335 8507 8727 2083
Size: 315 1776 172 220 5231
Fragments arranged by size:
5231 1776 1377 1095 954 363 315 220
172 159 126 57 30
PshAI GACnn'nnGTC
Cuts at: 1613 4940 1613
Size: 3327 8548
PsiI TTA'TAA
Cuts at: 9445 9445
Size: 11875
PspOMI G'GGCC_C
Cuts at: 3906 4088 4374 5352 7671 8534 8566 8844 3906
Size: 182 286 978 2319 863 32 278 6937
Fragments arranged by size:
6937 2319 978 863 286 278 182 32
PspXI vC'TCGA_Gb
Cuts at: 2288 6391 8634 2288
Size: 4103 2243 5529
Fragments arranged by size:
5529 4103 2243
PstI C_TGCA'G
Cuts at: 830 2376 2578 2718 2961 3039 3472 3606 3669
Size: 1546 202 140 243 78 433 134 63
Cuts at: 3669 3717 4518 4998 5208 6213 6459 6606 6705
Size: 48 801 480 210 1005 246 147 99
Cuts at: 6705 7077 7416 7521 7791 7980 8096 9227 830
Size: 372 339 105 270 189 116 1131 3478
Fragments arranged by size:
3478 1546 1131 1005 801 480 433 372
339 270 246 243 210 202 189 147
140 134 116 105 99 78 63 48
PvuI CG_AT'CG
Cuts at: 2001 2979 2001
Size: 978 10897
PvuII CAG'CTG
Cuts at: 2616 3034 3280 3380 3718 3835 4172 5716 6220
Size: 418 246 100 338 117 337 1544 504
Cuts at: 6220 6943 7708 7975 8000 8035 2616
Size: 723 765 267 25 35 6456
Fragments arranged by size:
6456 1544 765 723 504 418 338 337
267 246 117 100 35 25
SacI G_AGCT'C
Cuts at: 3255 6033 8957 3255
Size: 2778 2924 6173
Fragments arranged by size:
6173 2924 2778
SacII CC_GC'GG
Cuts at: 4734 7401 7830 4734
Size: 2667 429 8779
Fragments arranged by size:
8779 2667 429
SalI G'TCGA_C
Cuts at: 1936 8138 9217 1936
Size: 6202 1079 4594
Fragments arranged by size:
6202 4594 1079
SapI GCTCTTCn'nnn_
Cuts at: 3116 9012 3116
Size: 5896 5979
SbfI CC_TGCA'GG
Cuts at: 6213 6459 7077 9227 6213
Size: 246 618 2150 8861
Fragments arranged by size:
8861 2150 618 246
ScaI AGT'ACT
Cuts at: 10123 11835 10123
Size: 1712 10163
SexAI A'CCwGG_T
Cuts at: 6894 8337 6894
Size: 1443 10432
SfcI C'TryA_G
Cuts at: 826 1045 2372 2574 2714 2957 3035 3433 3468
Size: 219 1327 202 140 243 78 398 35
Cuts at: 3468 3602 3665 3713 4514 4994 5204 6209 6455
Size: 134 63 48 801 480 210 1005 246
Cuts at: 6455 6602 6701 7073 7412 7517 7787 7976 8092
Size: 147 99 372 339 105 270 189 116
Cuts at: 8092 9223 10358 11039 11230 826
Size: 1131 1135 681 191 1471
Fragments arranged by size:
1471 1327 1135 1131 1005 801 681 480
398 372 339 270 246 243 219 210
202 191 189 147 140 134 116 105
99 78 63 48 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 5315 7319 5315
Size: 2004 9871
SfoI GGC'GCC
Cuts at: 6452 7988 6452
Size: 1536 10339
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11875
SmaI CCC'GGG
Cuts at: 1872 2222 4523 5045 5402 7226 7814 7880 8540
Size: 350 2301 522 357 1824 588 66 660
Cuts at: 8540 9044 9056 9202 1872
Size: 504 12 146 4545
Fragments arranged by size:
4545 2301 1824 660 588 522 504 357
350 146 66 12
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 2381 4420 5061 6038 6269
Size: 197 926 345 93 2039 641 977 231
Cuts at: 6269 6391 7364 8477 8634 9979 10880 11142 820
Size: 122 973 1113 157 1345 901 262 1553
Fragments arranged by size:
2039 1553 1345 1113 977 973 926 901
641 345 262 231 197 157 122 93
SnaBI TAC'GTA
Cuts at: 493 9666 493
Size: 9173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11875
SphI G_CATG'C
Cuts at: 1132 2338 9233 1132
Size: 1206 6895 3774
Fragments arranged by size:
6895 3774 1206
SrfI GCCC'GGGC
Cuts at: 4523 7880 4523
Size: 3357 8518
SspI AAT'ATT
Cuts at: 5 52 8921 9799 5
Size: 47 8869 878 2081
Fragments arranged by size:
8869 2081 878 47
StuI AGG'CCT
Cuts at: 1517 3968 4157 4286 4919 5240 6809 7349 7610
Size: 2451 189 129 633 321 1569 540 261
Cuts at: 7610 7667 8084 8174 8702 1517
Size: 57 417 90 528 4690
Fragments arranged by size:
4690 2451 1569 633 540 528 417 321
261 189 129 90 57
StyI C'CwwG_G
Cuts at: 513 1065 2452 2538 2904 4545 4963 5664 6126
Size: 552 1387 86 366 1641 418 701 462
Cuts at: 6126 6531 6543 6855 8787 8934 9314 513
Size: 405 12 312 1932 147 380 3074
Fragments arranged by size:
3074 1932 1641 1387 701 552 462 418
405 380 366 312 147 86 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 3944 3961 4594 4814 4970 6134
Size: 1255 458 1906 17 633 220 156 1164
Cuts at: 6134 6502 6996 7232 8428 9902 9919 10072 10257
Size: 368 494 236 1196 1474 17 153 185
Cuts at: 10257 10702 325
Size: 445 1498
Fragments arranged by size:
1906 1498 1474 1255 1196 1164 633 494
458 445 368 236 220 185 156 153
17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 2247 2596
Size: 274 80 33 51 1443 81 189 349
Cuts at: 2596 8742 10121 11833 96
Size: 6146 1379 1712 138
Fragments arranged by size:
6146 1712 1443 1379 349 274 189 138
81 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2361 2393 4590 4622 11835 11867 2361
Size: 32 2197 32 7213 32 2369
Fragments arranged by size:
7213 2369 2197 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11875
XbaI T'CTAG_A
Cuts at: 9211 9211
Size: 11875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3359 4324 5641 6940 3359
Size: 965 1317 1299 8294
Fragments arranged by size:
8294 1317 1299 965
XhoI C'TCGA_G
Cuts at: 1943 2288 5061 6391 8634 1943
Size: 345 2773 1330 2243 5184
Fragments arranged by size:
5184 2773 2243 1330 345
XmaI C'CCGG_G
Cuts at: 1870 2220 4521 5043 5400 7224 7812 7878 8538
Size: 350 2301 522 357 1824 588 66 660
Cuts at: 8538 9042 9054 9200 1870
Size: 504 12 146 4545
Fragments arranged by size:
4545 2301 1824 660 588 522 504 357
350 146 66 12
XmnI GAAnn'nnTTC
Cuts at: 2189 2723 3611 5583 10004 2189
Size: 534 888 1972 4421 4060
Fragments arranged by size:
4421 4060 1972 888 534
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9683 276
Size: 53 83 186 936 8149 2468
Fragments arranged by size:
8149 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BstZ17I FspAI PacI PsrI RsrII SanDI
SwaI