Restriction map for
pFN21AA0300
(Circular) (Six-base) MAPSORT of: pFN21AA0300.seq Check: 9736 from: 1 to: 13222
pFN21AA0300 13222 bp
With 182 enzymes: SgfI *
December 26, 2011 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2046 4801 2046
Size: 2755 10467
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5046 11032 278
Size: 53 83 186 936 3510 5986 2468
Fragments arranged by size:
5986 3510 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 5766 10565 1937
Size: 1755 2074 4799 4594
Fragments arranged by size:
4799 4594 2074 1755
Acc65I G'GTAC_C
Cuts at: 4538 10543 4538
Size: 6005 7217
AclI AA'CG_TT
Cuts at: 1563 11349 11722 1563
Size: 9786 373 3063
Fragments arranged by size:
9786 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2718 6723 6960 7267 7679 8316 10088 10286
Size: 1086 4005 237 307 412 637 1772 198
Cuts at: 10286 10503 11285 12669 12937 1632
Size: 217 782 1384 268 1917
Fragments arranged by size:
4005 1917 1772 1384 1086 782 637 412
307 268 237 217 198
AfeI AGC'GCT
Cuts at: 2716 3071 7108 2716
Size: 355 4037 8830
Fragments arranged by size:
8830 4037 355
AflII C'TTAA_G
Cuts at: 820 1017 3703 820
Size: 197 2686 10339
Fragments arranged by size:
10339 2686 197
AflIII A'CryG_T
Cuts at: 1203 6815 12121 13098 1203
Size: 5612 5306 977 1327
Fragments arranged by size:
5612 5306 1327 977
AgeI A'CCGG_T
Cuts at: 12053 12053
Size: 13222
AhdI GACnn_n'nnGTC
Cuts at: 6691 6733 7629 11951 6691
Size: 42 896 4322 7962
Fragments arranged by size:
7962 4322 896 42
AleI CACnn'nnGTG
Cuts at: 1166 3098 9159 1166
Size: 1932 6061 5229
Fragments arranged by size:
6061 5229 1932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 9251 10116 10464 1184
Size: 8067 865 348 3942
Fragments arranged by size:
8067 3942 865 348
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5302 5334 8123 8155 5302
Size: 32 2789 32 10369
Fragments arranged by size:
10369 2789 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2059 2128 2249 3646 6155 7928 8312 9457 9621
Size: 69 121 1397 2509 1773 384 1145 164
Cuts at: 9621 9703 10965 12537 2059
Size: 82 1262 1572 2744
Fragments arranged by size:
2744 2509 1773 1572 1397 1262 1145 384
164 121 82 69
ApaI G_GGCC'C
Cuts at: 4167 4969 8621 10332 4167
Size: 802 3652 1711 7057
Fragments arranged by size:
7057 3652 1711 802
ApaLI G'TGCA_C
Cuts at: 11279 12435 11279
Size: 1156 12066
ApoI r'AATT_y
Cuts at: 1493 2883 3039 6580 9354 10401 10513 10531 10757
Size: 1390 156 3541 2774 1047 112 18 226
Cuts at: 10757 10909 13018 1493
Size: 152 2109 1697
Fragments arranged by size:
3541 2774 2109 1697 1390 1047 226 156
152 112 18
AseI AT'TA_AT
Cuts at: 160 1032 6525 7257 10413 10496 11776 160
Size: 872 5493 732 3156 83 1280 1606
Fragments arranged by size:
5493 3156 1606 1280 872 732 83
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13222
AvaI C'yCGr_G
Cuts at: 1870 1943 3612 4675 4972 5005 5473 5878 6235
Size: 73 1669 1063 297 33 468 405 357
Cuts at: 6235 6250 6430 6451 6838 8586 8623 8742 8780
Size: 15 180 21 387 1748 37 119 38
Cuts at: 8780 9599 10547 1870
Size: 819 948 4545
Fragments arranged by size:
4545 1748 1669 1063 948 819 468 405
387 357 297 180 119 73 38 37
33 21 15
BamHI G'GATC_C
Cuts at: 3591 10552 10919 3591
Size: 6961 367 5894
Fragments arranged by size:
6961 5894 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3113 4229 4440 4538 5098
Size: 325 217 627 1326 1116 211 98 560
Cuts at: 5098 6169 6473 8086 8593 9227 10031 10543 11998
Size: 1071 304 1613 507 634 804 512 1455
Cuts at: 11998 618
Size: 1842
Fragments arranged by size:
1842 1613 1455 1326 1116 1071 804 634
627 560 512 507 325 304 217 211
98
BanII G_rGCy'C
Cuts at: 1391 3189 4031 4167 4800 4969 5213 5315 5351
Size: 1798 842 136 633 169 244 102 36
Cuts at: 5351 6067 6272 6410 7022 8621 9098 9317 10332
Size: 716 205 138 612 1599 477 219 1015
Cuts at: 10332 10437 10541 1391
Size: 105 104 4072
Fragments arranged by size:
4072 1798 1599 1015 842 716 633 612
477 244 219 205 169 138 136 105
104 102 36
BbeI G_GCGC'C
Cuts at: 8090 10035 8090
Size: 1945 11277
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2954 3360 3898 4160 6324 6606 8798
Size: 971 1055 406 538 262 2164 282 2192
Cuts at: 8798 9114 10274 928
Size: 316 1160 3876
Fragments arranged by size:
3876 2192 2164 1160 1055 971 538 406
316 282 262
BbvCI CC'TCA_GC
Cuts at: 2375 3450 5403 6669 6883 7560 2375
Size: 1075 1953 1266 214 677 8037
Fragments arranged by size:
8037 1953 1266 1075 677 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5765 5799 8943 8977 9508
Size: 34 50 34 3744 34 3144 34 531
Cuts at: 9508 9542 11413 11447 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3744 3678 3144 1871 531 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5147 11114 12324 5147
Size: 5967 1210 6045
Fragments arranged by size:
6045 5967 1210
BclI T'GATC_A
Cuts at: 1548 2182 2523 1548
Size: 634 341 12247
Fragments arranged by size:
12247 634 341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2690 5096 7677
Size: 107 122 71 631 605 1018 2406 2581
Cuts at: 7677 8462 10040 11833 136
Size: 785 1578 1793 1525
Fragments arranged by size:
2581 2406 1793 1578 1525 1018 785 631
605 122 107 71
BglII A'GATC_T
Cuts at: 2205 3307 13217 2205
Size: 1102 9910 2210
Fragments arranged by size:
9910 2210 1102
BlpI GC'TnA_GC
Cuts at: 2490 7114 7667 10048 10639 2490
Size: 4624 553 2381 591 5073
Fragments arranged by size:
5073 4624 2381 591 553
Bme1580I G_kGCm'C
Cuts at: 1790 4167 4969 6172 6243 6478 8598 8621 10332
Size: 2377 802 1203 71 235 2120 23 1711
Cuts at: 10332 11283 12439 1790
Size: 951 1156 2573
Fragments arranged by size:
2573 2377 2120 1711 1203 1156 951 802
235 71 23
BmgBI CAC'GTC
Cuts at: 1318 4695 1318
Size: 3377 9845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4597 5310 5316
Size: 416 53 83 399 24 3180 713 6
Cuts at: 5316 5409 7861 8680 9660 10953 11911 442
Size: 93 2452 819 980 1293 958 1753
Fragments arranged by size:
3180 2452 1753 1293 980 958 819 713
416 399 93 83 53 24 6
BmtI G_CTAG'C
Cuts at: 1056 7113 1056
Size: 6057 7165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 4575 6053 6864 7036 9523 2449
Size: 2126 1478 811 172 2487 6148
Fragments arranged by size:
6148 2487 2126 1478 811 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2656 2774 3831 4272 4395 5335 5586 5829
Size: 1417 118 1057 441 123 940 251 243
Cuts at: 5829 6009 6051 6161 6417 9434 9566 9603 10229
Size: 180 42 110 256 3017 132 37 626
Cuts at: 10229 10413 11882 1239
Size: 184 1469 2579
Fragments arranged by size:
3017 2579 1469 1417 1057 940 626 441
256 251 243 184 180 132 123 118
110 42 37
Bpu10I CC'TnA_GC
Cuts at: 2375 3450 4319 4534 5403 6372 6669 6883 7560
Size: 1075 869 215 869 969 297 214 677
Cuts at: 7560 7983 9895 2375
Size: 423 1912 5702
Fragments arranged by size:
5702 1912 1075 969 869 869 677 423
297 215 214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2807 3081 3491 3560 4417 6148 7658 8677 9214
Size: 274 410 69 857 1731 1510 1019 537
Cuts at: 9214 9487 11347 12212 12510 2807
Size: 273 1860 865 298 3519
Fragments arranged by size:
3519 1860 1731 1510 1019 865 857 537
410 298 274 273 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3613 5847 6024 6330 9293 11885 882
Size: 617 2114 2234 177 306 2963 2592 2219
Fragments arranged by size:
2963 2592 2234 2219 2114 617 306 177
BsaAI yAC'GTr
Cuts at: 493 1204 6818 11013 493
Size: 711 5614 4195 2702
Fragments arranged by size:
5614 4195 2702 711
BsaBI GATnn'nnATC
Cuts at: 5926 9802 13216 5926
Size: 3876 3414 5932
Fragments arranged by size:
5932 3876 3414
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5043 8087 8507 10032
Size: 53 83 186 936 3510 3044 420 1525
Cuts at: 10032 11029 11411 12046 275
Size: 997 382 635 1451
Fragments arranged by size:
3510 3044 1525 1451 997 936 635 420
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3594 3984 11655 12053 12327 12474 12885 3594
Size: 390 7671 398 274 147 411 3931
Fragments arranged by size:
7671 3931 411 398 390 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2451 2481 2884 2914 3618
Size: 30 165 30 1048 30 403 30 704
Cuts at: 3618 3648 4842 4872 5302 5332 6593 6623 7299
Size: 30 1194 30 430 30 1261 30 676
Cuts at: 7299 7329 7791 7821 1178
Size: 30 462 30 6579
Fragments arranged by size:
6579 1261 1194 1048 704 676 462 430
403 165 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2892 3269 4715 4862 5144 5689 6436
Size: 850 856 377 1446 147 282 545 747
Cuts at: 6436 6469 6732 7462 7548 8503 8506 8509 1186
Size: 33 263 730 86 955 3 3 5899
Fragments arranged by size:
5899 1446 955 856 850 747 730 545
377 282 263 147 86 33 3 3
BseYI C'CCAG_C
Cuts at: 2233 2389 2617 2741 3034 4031 4718 5325 5665
Size: 156 228 124 293 997 687 607 340
Cuts at: 5665 5750 5798 6501 6993 7727 8810 10277 10282
Size: 85 48 703 492 734 1083 1467 5
Cuts at: 10282 12425 12921 2233
Size: 2143 496 2534
Fragments arranged by size:
2534 2143 1467 1083 997 734 703 687
607 496 492 340 293 228 156 124
85 48 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2018 3257 7101 10061 10076 10508 1163
Size: 573 282 1239 3844 2960 15 432 3877
Fragments arranged by size:
3877 3844 2960 1239 573 432 282 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4438 8251 10672 11433 12461 1540
Size: 275 186 2437 3813 2421 761 1028 2301
Fragments arranged by size:
3813 2437 2421 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5608 6067 6373 6410 7022 9098 9531 11283 11368
Size: 459 306 37 612 2076 433 1752 85
Cuts at: 11368 12439 5608
Size: 1071 6391
Fragments arranged by size:
6391 2076 1752 1071 612 459 433 306
85 37
BsmI GAATG_Cn'
Cuts at: 3825 7720 8607 10733 10826 3825
Size: 3895 887 2126 93 6221
Fragments arranged by size:
6221 3895 2126 887 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6648 8258 8906 9178 6648
Size: 1610 648 272 10692
Fragments arranged by size:
10692 1610 648 272
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3189 4031 4167 4800 4969 5213 5315
Size: 399 1399 842 136 633 169 244 102
Cuts at: 5315 5351 5608 6067 6172 6243 6272 6373 6410
Size: 36 257 459 105 71 29 101 37
Cuts at: 6410 6478 7022 8598 8621 9098 9317 9531 10332
Size: 68 544 1576 23 477 219 214 801
Cuts at: 10332 10437 10541 11283 11368 12439 1391
Size: 105 104 742 85 1071 2174
Fragments arranged by size:
2174 1576 1399 1071 842 801 742 633
544 477 459 399 257 244 219 214
169 136 105 105 104 102 101 85
71 68 37 36 29 23
BspEI T'CCGG_A
Cuts at: 3594 3984 12885 3594
Size: 390 8901 3931
Fragments arranged by size:
8901 3931 390
BspHI T'CATG_A
Cuts at: 6595 9264 11111 12841 6595
Size: 2669 1847 1730 6976
Fragments arranged by size:
6976 2669 1847 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 4801 5652 7224 7992 10577 844
Size: 1202 2755 851 1572 768 2585 3489
Fragments arranged by size:
3489 2755 2585 1572 1202 851 768
BsrBI CCG'CTC
Cuts at: 1394 1823 3119 3699 5738 5981 9441 11109 1394
Size: 429 1296 580 2039 243 3460 1668 3507
Fragments arranged by size:
3507 3460 2039 1668 1296 580 429 243
BsrDI GCAATG_nn'
Cuts at: 65 1248 2923 3417 11717 11891 65
Size: 1183 1675 494 8300 174 1396
Fragments arranged by size:
8300 1675 1396 1183 494 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4437 4652 8107 9051 9224 11866 12053
Size: 110 2627 215 3455 944 173 2642 187
Cuts at: 12053 1700
Size: 2869
Fragments arranged by size:
3455 2869 2642 2627 944 215 187 173
110
BsrGI T'GTAC_A
Cuts at: 96 3529 3771 10237 10350 96
Size: 3433 242 6466 113 2968
Fragments arranged by size:
6466 3433 2968 242 113
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13222
BssSI C'ACGA_G
Cuts at: 3194 4766 6711 7075 7823 7933 10106 11282 12294
Size: 1572 1945 364 748 110 2173 1176 1012
Cuts at: 12294 3194
Size: 4122
Fragments arranged by size:
4122 2173 1945 1572 1176 1012 748 364
110
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 13222
BstBI TT'CG_AA
Cuts at: 12026 12026
Size: 13222
BstEII G'GTnAC_C
Cuts at: 1184 2621 6071 8714 9564 1184
Size: 1437 3450 2643 850 4842
Fragments arranged by size:
4842 3450 2643 1437 850
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2485 3232 5038 5962 8185 10207 2233
Size: 252 747 1806 924 2223 2022 5248
Fragments arranged by size:
5248 2223 2022 1806 924 747 252
BstYI r'GATC_y
Cuts at: 1972 2205 2427 3307 3591 4070 5393 6772 9700
Size: 233 222 880 284 479 1323 1379 2928
Cuts at: 9700 10552 10919 11304 11321 12773 12859 12871 13217
Size: 852 367 385 17 1452 86 12 346
Cuts at: 13217 1972
Size: 1977
Fragments arranged by size:
2928 1977 1452 1379 1323 880 852 479
385 367 346 284 233 222 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3142 3281 5972 3142
Size: 139 2691 10392
Fragments arranged by size:
10392 2691 139
BtgI C'CryG_G
Cuts at: 513 1065 3225 4324 7119 8142 10054 513
Size: 552 2160 1099 2795 1023 1912 3681
Fragments arranged by size:
3681 2795 2160 1912 1099 1023 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5796 6678 487
Size: 683 56 4570 882 7031
Fragments arranged by size:
7031 4570 882 683 56
BtsI GCAGTG_nn'
Cuts at: 2501 3210 5383 6241 6373 7800 8307 8776 10738
Size: 709 2173 858 132 1427 507 469 1962
Cuts at: 10738 11532 11552 2501
Size: 794 20 4171
Fragments arranged by size:
4171 2173 1962 1427 858 794 709 507
469 132 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4078 4113 5398 5433 7976 8011 529
Size: 35 3514 35 1285 35 2543 35 5740
Fragments arranged by size:
5740 3514 2543 1285 35 35 35 35
DraI TTT'AAA
Cuts at: 7411 10526 7411
Size: 3115 10107
DraIII CAC_nnn'GTG
Cuts at: 3682 9961 3682
Size: 6279 6943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4178 4833 5477 8712 8753 12229 809
Size: 507 2862 655 644 3235 41 3476 1802
Fragments arranged by size:
3476 3235 2862 1802 655 644 507 41
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2785 2992
Size: 54 1425 50 275 18 285 670 207
Cuts at: 2992 3207 3249 3619 3743 4299 4435 4650 5367
Size: 215 42 370 124 556 136 215 717
Cuts at: 5367 5786 6226 6930 8175 8241 9039 9246 10669
Size: 419 440 704 1245 66 798 207 1423
Cuts at: 10669 11558 13187 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1423 1245 889 798 717 704
670 556 440 419 370 285 275 215
215 207 207 136 124 66 54 50
43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 4435 10669 1537
Size: 275 2623 6234 4090
Fragments arranged by size:
6234 4090 2623 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 2393 3369 3400 3658 4544 4805 4920
Size: 959 81 976 31 258 886 261 115
Cuts at: 4920 5032 5109 5719 6783 6994 7595 7655 8378
Size: 112 77 610 1064 211 601 60 723
Cuts at: 8378 9536 11152 1353
Size: 1158 1616 3423
Fragments arranged by size:
3423 1616 1158 1064 976 959 886 723
610 601 261 258 211 115 112 81
77 60 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3211 4063 4352 4865 7155 11809
Size: 1064 138 1622 852 289 513 2290 4654
Cuts at: 11809 12183 12329 387
Size: 374 146 1280
Fragments arranged by size:
4654 2290 1622 1280 1064 852 513 374
289 146 138
EcoICRI GAG'CTC
Cuts at: 6065 6408 7020 9096 6065
Size: 343 612 2076 10191
Fragments arranged by size:
10191 2076 612 343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2656 2718 2774 3831 4272 4395 5335
Size: 393 1024 62 56 1057 441 123 940
Cuts at: 5335 5586 5829 6009 6051 6161 6417 6723 6960
Size: 251 243 180 42 110 256 306 237
Cuts at: 6960 7267 7679 8316 9434 9566 9603 10088 10229
Size: 307 412 637 1118 132 37 485 141
Cuts at: 10229 10286 10413 10503 11285 11882 12669 12937 1239
Size: 57 127 90 782 597 787 268 1524
Fragments arranged by size:
1524 1118 1057 1024 940 787 782 637
597 485 441 412 393 307 306 268
256 251 243 237 180 141 132 127
123 110 90 62 57 56 42 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 4533 6140 6451 7986 9582 2225
Size: 2308 1607 311 1535 1596 5865
Fragments arranged by size:
5865 2308 1607 1596 1535 311
EcoO109I rG'GnC_Cy
Cuts at: 2336 2692 3274 3583 3790 4415 4855 4965 4966
Size: 356 582 309 207 625 440 110 1
Cuts at: 4966 4976 5197 5984 6459 7071 7588 7702 7828
Size: 10 221 787 475 612 517 114 126
Cuts at: 7828 7860 8617 8618 8667 8755 9618 10140 10329
Size: 32 757 1 49 88 863 522 189
Cuts at: 10329 10481 2336
Size: 152 5077
Fragments arranged by size:
5077 863 787 757 625 612 582 522
517 475 440 356 309 221 207 189
152 126 114 110 88 49 32 10
1 1
EcoRI G'AATT_C
Cuts at: 2883 3039 9354 10513 10531 10909 2883
Size: 156 6315 1159 18 378 5196
Fragments arranged by size:
6315 5196 1159 378 156 18
EcoRV GAT'ATC
Cuts at: 8896 8896
Size: 13222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2971 3484 3877 2971
Size: 513 393 12316
Fragments arranged by size:
12316 513 393
FspI TGC'GCA
Cuts at: 11728 11728
Size: 13222
HaeII r_GCGC'y
Cuts at: 1678 2718 3073 7110 8090 9525 10035 12369 1678
Size: 1040 355 4037 980 1435 510 2334 2531
Fragments arranged by size:
4037 2531 2334 1435 1040 980 510 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2451 2483 2884 2916 2945
Size: 32 135 32 879 32 401 32 29
Cuts at: 2945 2977 3320 3352 5147 5179 5456 5488 5528
Size: 32 343 32 1795 32 277 32 40
Cuts at: 5528 5560 5684 5696 5716 5728 6143 6175 6338
Size: 32 124 12 20 12 415 32 163
Cuts at: 6338 6370 6593 6625 6677 6709 6719 6751 7006
Size: 32 223 32 52 32 10 32 255
Cuts at: 7006 7038 7615 7647 9265 9297 9926 9958 11863
Size: 32 577 32 1618 32 629 32 1905
Cuts at: 11863 11895 11937 11969 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1905 1795 1618 879 629 577 415
401 343 277 255 223 163 135 124
52 42 40 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 29
20 12 12 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3288 4357 7693 10027 10566 10812
Size: 988 281 1350 1069 3336 2334 539 246
Cuts at: 10812 669
Size: 3079
Fragments arranged by size:
3336 3079 2334 1350 1069 988 539 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 13222
HpaI GTT'AAC
Cuts at: 3288 10812 3288
Size: 7524 5698
KasI G'GCGC_C
Cuts at: 8086 10031 8086
Size: 1945 11277
KpnI G_GTAC'C
Cuts at: 4542 10547 4542
Size: 6005 7217
MfeI C'AATT_G
Cuts at: 10821 10821
Size: 13222
MluI A'CGCG_T
Cuts at: 13098 13098
Size: 13222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2437 3582 4130 4195 4677 5321 12336 12520
Size: 1132 1145 548 65 482 644 7015 184
Cuts at: 12520 1305
Size: 2007
Fragments arranged by size:
7015 2007 1145 1132 644 548 482 184
65
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3209 3251 4301 5369 6228
Size: 54 1425 343 1377 42 1050 1068 859
Cuts at: 6228 6932 8177 8243 9041 9248 10
Size: 704 1245 66 798 207 3984
Fragments arranged by size:
3984 1425 1377 1245 1068 1050 859 798
704 343 207 66 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 3098 3126 3230 4061 5619 8141
Size: 648 1197 735 28 104 831 1558 2522
Cuts at: 8141 9159 10059 10098 10446 11180 11539 11698 518
Size: 1018 900 39 348 734 359 159 2042
Fragments arranged by size:
2522 2042 1558 1197 1018 900 831 735
734 648 359 348 159 104 39 28
MspA1I CmG'CkG
Cuts at: 1606 2101 2146 2449 3643 3878 4326 5589 5894
Size: 495 45 303 1194 235 448 1263 305
Cuts at: 5894 6011 7135 7265 7804 7850 8272 8333 8851
Size: 117 1124 130 539 46 422 61 518
Cuts at: 8851 9016 9755 10090 10638 11315 12463 12708 1606
Size: 165 739 335 548 677 1148 245 2120
Fragments arranged by size:
2120 1263 1194 1148 1124 739 677 548
539 518 495 448 422 335 305 303
245 235 165 130 117 61 46 45
NaeI GCC'GGC
Cuts at: 4439 9226 4439
Size: 4787 8435
NarI GG'CG_CC
Cuts at: 8087 10032 8087
Size: 1945 11277
NcoI C'CATG_G
Cuts at: 513 1065 7119 513
Size: 552 6054 6616
Fragments arranged by size:
6616 6054 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 13222
NgoMIV G'CCGG_C
Cuts at: 4437 9224 4437
Size: 4787 8435
NheI G'CTAG_C
Cuts at: 1052 7109 1052
Size: 6057 7165
NotI GC'GGCC_GC
Cuts at: 10669 10669
Size: 13222
NspI r_CATG'y
Cuts at: 1132 1229 6225 7996 8140 9475 10580 12125 1132
Size: 97 4996 1771 144 1335 1105 1545 2229
Fragments arranged by size:
4996 2229 1771 1545 1335 1105 144 97
PasI CC'CwG_GG
Cuts at: 1348 4144 4160 4961 5720 5817 8365 8557 1348
Size: 2796 16 801 759 97 2548 192 6013
Fragments arranged by size:
6013 2796 2548 801 759 192 97 16
PciI A'CATG_T
Cuts at: 12121 12121
Size: 13222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4214 1108
Size: 3106 10116
PfoI T'CCnGG_A
Cuts at: 3338 4288 4338 4411 8907 9140 9450 9625 3338
Size: 950 50 73 4496 233 310 175 6935
Fragments arranged by size:
6935 4496 950 310 233 175 73 50
PmeI GTTT'AAAC
Cuts at: 10526 10526
Size: 13222
PmlI CAC'GTG
Cuts at: 6818 6818
Size: 13222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5302 5334 9389 9421 11291 11323 12804 12836 5302
Size: 32 4055 32 1870 32 1481 32 5688
Fragments arranged by size:
5688 4055 1870 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2336 3274 3583 4415 4976 5984 7588 7828 7860
Size: 938 309 832 561 1008 1604 240 32
Cuts at: 7860 8667 8755 10481 2336
Size: 807 88 1726 5077
Fragments arranged by size:
5077 1726 1604 1008 938 832 807 561
309 240 88 32
PshAI GACnn'nnGTC
Cuts at: 1613 7949 1613
Size: 6336 6886
PsiI TTA'TAA
Cuts at: 2565 10792 2565
Size: 8227 4995
PspOMI G'GGCC_C
Cuts at: 4163 4965 8617 10328 4163
Size: 802 3652 1711 7057
Fragments arranged by size:
7057 3652 1711 802
PspXI vC'TCGA_Gb
Cuts at: 6838 6838
Size: 13222
PstI C_TGCA'G
Cuts at: 830 2064 2076 2133 3883 4691 5194 6319 9206
Size: 1234 12 57 1750 808 503 1125 2887
Cuts at: 9206 9497 10574 830
Size: 291 1077 3478
Fragments arranged by size:
3478 2887 1750 1234 1125 1077 808 503
291 57 12
PvuI CG_AT'CG
Cuts at: 2001 8251 2001
Size: 6250 6972
PvuII CAG'CTG
Cuts at: 2146 2449 3643 3878 5589 6011 7265 7850 8272
Size: 303 1194 235 1711 422 1254 585 422
Cuts at: 8272 8333 10090 2146
Size: 61 1757 5278
Fragments arranged by size:
5278 1757 1711 1254 1194 585 422 422
303 235 61
SacI G_AGCT'C
Cuts at: 6067 6410 7022 9098 6067
Size: 343 612 2076 10191
Fragments arranged by size:
10191 2076 612 343
SacII CC_GC'GG
Cuts at: 4327 4327
Size: 13222
SalI G'TCGA_C
Cuts at: 1936 10564 1936
Size: 8628 4594
SanDI GG'GwC_CC
Cuts at: 3583 4976 3583
Size: 1393 11829
SapI GCTCTTCn'nnn_
Cuts at: 3400 4805 9536 3400
Size: 1405 4731 7086
Fragments arranged by size:
7086 4731 1405
SbfI CC_TGCA'GG
Cuts at: 2076 10574 2076
Size: 8498 4724
ScaI AGT'ACT
Cuts at: 4607 11470 13182 4607
Size: 6863 1712 4647
Fragments arranged by size:
6863 4647 1712
SfcI C'TryA_G
Cuts at: 826 1045 2060 2072 2129 3879 4687 5190 5763
Size: 219 1015 12 57 1750 808 503 573
Cuts at: 5763 5908 6315 7364 9057 9202 9493 10570 11705
Size: 145 407 1049 1693 145 291 1077 1135
Cuts at: 11705 12386 12577 826
Size: 681 191 1471
Fragments arranged by size:
1750 1693 1471 1135 1077 1049 1015 808
681 573 503 407 291 219 191 145
145 57 12
SfoI GGC'GCC
Cuts at: 8088 10033 8088
Size: 1945 11277
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13222
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13222
SmaI CCC'GGG
Cuts at: 1872 4677 4974 5880 6252 6432 10549 1872
Size: 2805 297 906 372 180 4117 4545
Fragments arranged by size:
4545 4117 2805 906 372 297 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2822 3096 3506 3539 3703 4396
Size: 197 926 879 274 410 33 164 693
Cuts at: 4396 5473 6163 6838 7673 8692 9193 9466 11326
Size: 1077 690 675 835 1019 501 273 1860
Cuts at: 11326 12227 12489 820
Size: 901 262 1553
Fragments arranged by size:
1860 1553 1077 1019 926 901 879 835
693 690 675 501 410 274 273 262
197 164 33
SnaBI TAC'GTA
Cuts at: 493 11013 493
Size: 10520 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13222
SphI G_CATG'C
Cuts at: 1132 9475 10580 1132
Size: 8343 1105 3774
Fragments arranged by size:
8343 3774 1105
SspI AAT'ATT
Cuts at: 5 52 10459 11146 5
Size: 47 10407 687 2081
Fragments arranged by size:
10407 2081 687 47
StuI AGG'CCT
Cuts at: 1517 2677 4987 5343 6621 1517
Size: 1160 2310 356 1278 8118
Fragments arranged by size:
8118 2310 1278 1160 356
StyI C'CwwG_G
Cuts at: 513 1065 2226 2610 2706 3459 3728 3793 4167
Size: 552 1161 384 96 753 269 65 374
Cuts at: 4167 5031 5202 7119 7432 8651 10332 10661 513
Size: 864 171 1917 313 1219 1681 329 3074
Fragments arranged by size:
3074 1917 1681 1219 1161 864 753 552
384 374 329 313 269 171 96 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 2353 2609 2640 4069 5272 5303
Size: 1255 450 323 256 31 1429 1203 31
Cuts at: 5303 6396 6614 6875 9171 9679 11249 11266 11419
Size: 1093 218 261 2296 508 1570 17 153
Cuts at: 11419 11604 12049 325
Size: 185 445 1498
Fragments arranged by size:
2296 1570 1498 1429 1255 1203 1093 508
450 445 323 261 256 218 185 153
31 31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2210 3529
Size: 274 80 33 51 1443 159 74 1319
Cuts at: 3529 3771 4605 10237 10350 11468 13180 96
Size: 242 834 5632 113 1118 1712 138
Fragments arranged by size:
5632 1712 1443 1319 1118 834 274 242
159 138 113 80 74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4842 4874 5278 5310 7791 7823 8416 8448 9897
Size: 32 404 32 2481 32 593 32 1449
Cuts at: 9897 9929 13182 13214 4842
Size: 32 3253 32 4850
Fragments arranged by size:
4850 3253 2481 1449 593 404 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 10290 1316
Size: 8974 4248
XbaI T'CTAG_A
Cuts at: 2845 6575 10558 2845
Size: 3730 3983 5509
Fragments arranged by size:
5509 3983 3730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2749 3466 4622 4790 5686 7507 2749
Size: 717 1156 168 896 1821 8464
Fragments arranged by size:
8464 1821 1156 896 717 168
XhoI C'TCGA_G
Cuts at: 1943 5473 6838 1943
Size: 3530 1365 8327
Fragments arranged by size:
8327 3530 1365
XmaI C'CCGG_G
Cuts at: 1870 4675 4972 5878 6250 6430 10547 1870
Size: 2805 297 906 372 180 4117 4545
Fragments arranged by size:
4545 4117 2805 906 372 297 180
XmnI GAAnn'nnTTC
Cuts at: 3963 7617 11351 3963
Size: 3654 3734 5834
Fragments arranged by size:
5834 3734 3654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5044 11030 276
Size: 53 83 186 936 3510 5986 2468
Fragments arranged by size:
5986 3510 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI
NruI NsiI PacI PsrI RsrII SexAI SfiI SrfI
SwaI