Restriction map for
pFN21AA0288
(Circular) (Six-base) MAPSORT of: pFN21AA0288.seq Check: 9633 from: 1 to: 7957
pFN21AA0288 7957 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3664 4016 3664
Size: 352 7605
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5767 278
Size: 53 83 186 936 4231 2468
Fragments arranged by size:
4231 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 5300 1937
Size: 732 2631 4594
Fragments arranged by size:
4594 2631 732
Acc65I G'GTAC_C
Cuts at: 4866 5278 4866
Size: 412 7545
AclI AA'CG_TT
Cuts at: 1563 6084 6457 1563
Size: 4521 373 3063
Fragments arranged by size:
4521 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3757 4013 4394 4720 6020 7404 7672 1632
Size: 2125 256 381 326 1300 1384 268 1917
Fragments arranged by size:
2125 1917 1384 1300 381 326 268 256
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7760
AflIII A'CryG_T
Cuts at: 1203 4581 6856 7833 1203
Size: 3378 2275 977 1327
Fragments arranged by size:
3378 2275 1327 977
AgeI A'CCGG_T
Cuts at: 6788 6788
Size: 7957
AhdI GACnn_n'nnGTC
Cuts at: 3349 6686 3349
Size: 3337 4620
AleI CACnn'nnGTG
Cuts at: 1166 2513 1166
Size: 1347 6610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7957
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2156 2188 2156
Size: 32 7925
AlwNI CAG_nnn'CTG
Cuts at: 2215 2383 2431 2458 2741 3433 3442 3472 3508
Size: 168 48 27 283 692 9 30 36
Cuts at: 3508 3607 3766 4201 5700 7272 2215
Size: 99 159 435 1499 1572 2900
Fragments arranged by size:
2900 1572 1499 692 435 283 168 159
99 48 36 30 27 9
ApaI G_GGCC'C
Cuts at: 4656 4656
Size: 7957
ApaLI G'TGCA_C
Cuts at: 4003 4145 4298 4526 6014 7170 4003
Size: 142 153 228 1488 1156 4790
Fragments arranged by size:
4790 1488 1156 228 153 142
ApoI r'AATT_y
Cuts at: 1493 2534 5266 5492 5644 7753 1493
Size: 1041 2732 226 152 2109 1697
Fragments arranged by size:
2732 2109 1697 1041 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6511 160
Size: 872 5479 1606
Fragments arranged by size:
5479 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7957
AvaI C'yCGr_G
Cuts at: 1870 1943 3888 4031 4919 5282 1870
Size: 73 1945 143 888 363 4545
Fragments arranged by size:
4545 1945 888 363 143 73
BamHI G'GATC_C
Cuts at: 3807 4047 5287 5654 3807
Size: 240 1240 367 6110
Fragments arranged by size:
6110 1240 367 240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2412 3173 4866 5278 6733
Size: 325 217 627 625 761 1693 412 1455
Cuts at: 6733 618
Size: 1842
Fragments arranged by size:
1842 1693 1455 761 627 625 412 325
217
BanII G_rGCy'C
Cuts at: 1391 2012 2229 3591 3621 3690 3720 3942 4633
Size: 621 217 1362 30 69 30 222 691
Cuts at: 4633 4656 5235 5276 1391
Size: 23 579 41 4072
Fragments arranged by size:
4072 1362 691 621 579 222 217 69
41 30 30 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4342 928
Size: 971 2443 4543
Fragments arranged by size:
4543 2443 971
BbvCI CC'TCA_GC
Cuts at: 3818 3818
Size: 7957
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6148 6182 1903
Size: 34 4211 34 3678
Fragments arranged by size:
4211 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5849 7059 5849
Size: 1210 6747
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7957
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 3550 4089
Size: 107 122 71 631 605 466 1412 539
Cuts at: 4089 4322 4896 6568 136
Size: 233 574 1672 1525
Fragments arranged by size:
1672 1525 1412 631 605 574 539 466
233 122 107 71
BglII A'GATC_T
Cuts at: 7952 7952
Size: 7957
BlpI GC'TnA_GC
Cuts at: 5374 5374
Size: 7957
Bme1580I G_kGCm'C
Cuts at: 1790 2148 3104 4007 4149 4302 4530 4649 4656
Size: 358 956 903 142 153 228 119 7
Cuts at: 4656 6018 7174 1790
Size: 1362 1156 2573
Fragments arranged by size:
2573 1362 1156 956 903 358 228 153
142 119 7
BmgBI CAC'GTC
Cuts at: 1318 4525 1318
Size: 3207 4750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3394 3398 3747
Size: 416 53 83 399 24 1977 4 349
Cuts at: 3747 4528 5688 6646 442
Size: 781 1160 958 1753
Fragments arranged by size:
1977 1753 1160 958 781 416 399 349
83 53 24 4
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7957
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 4318 2320
Size: 1998 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2426 2453 3131 3284 3494 3815 4148 6617
Size: 1187 27 678 153 210 321 333 2469
Cuts at: 6617 1239
Size: 2579
Fragments arranged by size:
2579 2469 1187 678 333 321 210 153
27
Bpu10I CC'TnA_GC
Cuts at: 3194 3818 3194
Size: 624 7333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 2453 6082 6947 7245 2222
Size: 231 3629 865 298 2934
Fragments arranged by size:
3629 2934 865 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2037 5212 6620 882
Size: 617 538 3175 1408 2219
Fragments arranged by size:
3175 2219 1408 617 538
BsaAI yAC'GTr
Cuts at: 493 1204 5748 493
Size: 711 4544 2702
Fragments arranged by size:
4544 2702 711
BsaBI GATnn'nnATC
Cuts at: 2270 4508 7951 2270
Size: 2238 3443 2276
Fragments arranged by size:
3443 2276 2238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2706 3120 3833 4953
Size: 53 83 186 936 1173 414 713 1120
Cuts at: 4953 5764 6146 6781 275
Size: 811 382 635 1451
Fragments arranged by size:
1451 1173 1120 936 811 713 635 414
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2568 2897 6390 6788 7062 7209 7620 2568
Size: 329 3493 398 274 147 411 2905
Fragments arranged by size:
3493 2905 411 398 329 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2906 2936 3300 3330 4436
Size: 30 165 30 1503 30 364 30 1106
Cuts at: 4436 4466 4832 4862 1178
Size: 30 366 30 4273
Fragments arranged by size:
4273 1503 1106 366 364 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3587 3596 3599 4145 4433 5243 5258 1186
Size: 2401 9 3 546 288 810 15 3885
Fragments arranged by size:
3885 2401 810 546 288 15 9 3
BseYI C'CCAG_C
Cuts at: 2090 2903 2966 3070 4545 4568 4981 7160 7656
Size: 813 63 104 1475 23 413 2179 496
Cuts at: 7656 2090
Size: 2391
Fragments arranged by size:
2391 2179 1475 813 496 413 104 63
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2899 3360 3692 3953 1163
Size: 573 1163 461 332 261 5167
Fragments arranged by size:
5167 1163 573 461 332 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3024 4758 4903 5407 6168 7196
Size: 275 186 1023 1734 145 504 761 1028
Cuts at: 7196 1540
Size: 2301
Fragments arranged by size:
2301 1734 1028 1023 761 504 275 186
145
BsiHKAI G_wGCw'C
Cuts at: 2012 2229 2337 3200 3310 3591 3600 4007 4038
Size: 217 108 863 110 281 9 407 31
Cuts at: 4038 4149 4302 4427 4530 6018 6103 7174 2012
Size: 111 153 125 103 1488 85 1071 2795
Fragments arranged by size:
2795 1488 1071 863 407 281 217 153
125 111 110 108 103 85 31 9
BsmI GAATG_Cn'
Cuts at: 5468 5561 5468
Size: 93 7864
BsmBI CGTCTCn'nnnn_
Cuts at: 2975 3569 4070 4707 5159 2975
Size: 594 501 637 452 5773
Fragments arranged by size:
5773 637 594 501 452
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2148 2229 2337 3104 3200 3310
Size: 399 222 136 81 108 767 96 110
Cuts at: 3310 3591 3600 3621 3690 3720 3942 4007 4038
Size: 281 9 21 69 30 222 65 31
Cuts at: 4038 4149 4302 4427 4530 4633 4649 4656 5235
Size: 111 153 125 103 103 16 7 579
Cuts at: 5235 5276 6018 6103 7174 1391
Size: 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 767 742 579 399 281 222
222 153 136 125 111 110 108 103
103 96 85 81 69 65 41 31
30 21 16 9 7
BspEI T'CCGG_A
Cuts at: 2897 7620 2897
Size: 4723 3234
BspHI T'CATG_A
Cuts at: 5846 7576 5846
Size: 1730 6227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 3664 4016 5312 844
Size: 1319 1501 352 1296 3489
Fragments arranged by size:
3489 1501 1319 1296 352
BsrBI CCG'CTC
Cuts at: 1394 1823 2661 3026 3214 5844 1394
Size: 429 838 365 188 2630 3507
Fragments arranged by size:
3507 2630 838 429 365 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 6452 6626 65
Size: 1183 5204 174 1396
Fragments arranged by size:
5204 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2194 3086 3274 3630 3665 3848 4191
Size: 110 384 892 188 356 35 183 343
Cuts at: 4191 4688 6601 6788 1700
Size: 497 1913 187 2869
Fragments arranged by size:
2869 1913 892 497 384 356 343 188
187 183 110 35
BsrGI T'GTAC_A
Cuts at: 96 4578 96
Size: 4482 3475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7957
BssSI C'ACGA_G
Cuts at: 2294 2315 2330 3009 3971 6017 7029 2294
Size: 21 15 679 962 2046 1012 3222
Fragments arranged by size:
3222 2046 1012 962 679 21 15
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2215 2383 2458 3508 3766 794
Size: 1421 168 75 1050 258 4985
Fragments arranged by size:
4985 1421 1050 258 168 75
BstBI TT'CG_AA
Cuts at: 6761 6761
Size: 7957
BstEII G'GTnAC_C
Cuts at: 1184 5170 1184
Size: 3986 3971
BstXI CCAn_nnnn'nTGG
Cuts at: 2027 2098 2027
Size: 71 7886
BstYI r'GATC_y
Cuts at: 1972 2253 2265 3807 4047 4503 5070 5287 5654
Size: 281 12 1542 240 456 567 217 367
Cuts at: 5654 6039 6056 7508 7594 7606 7952 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1542 1452 567 456 385 367 346
281 240 217 86 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2109 2148 2499 3722 3837 4108 4431
Size: 552 1044 39 351 1223 115 271 323
Cuts at: 4431 4455 4461 4531 4707 4809 5052 5178 5193
Size: 24 6 70 176 102 243 126 15
Cuts at: 5193 5235 513
Size: 42 3235
Fragments arranged by size:
3235 1223 1044 552 351 323 271 243
176 126 115 102 70 42 39 24
15 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2257 3030 3714 487
Size: 683 56 1031 773 684 4730
Fragments arranged by size:
4730 1031 773 684 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 2177 2581 2835 3308 5473 6267 6287 2148
Size: 29 404 254 473 2165 794 20 3818
Fragments arranged by size:
3818 2165 794 473 404 254 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7957
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4813 4848 529
Size: 35 4249 35 3638
Fragments arranged by size:
4249 3638 35 35
DraI TTT'AAA
Cuts at: 5261 5261
Size: 7957
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4571 6964 809
Size: 507 3255 2393 1802
Fragments arranged by size:
3255 2393 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2106 2361
Size: 54 1425 50 275 18 208 68 255
Cuts at: 2361 2502 2784 3021 3871 4359 4458 4900 5404
Size: 141 282 237 850 488 99 442 504
Cuts at: 5404 6293 7922 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 850 504 488 442 282
275 255 237 208 141 99 68 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3021 4900 5404 1537
Size: 275 1209 1879 504 4090
Fragments arranged by size:
4090 1879 1209 504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3246 3702 3776 5235 5887 1353
Size: 1893 456 74 1459 652 3423
Fragments arranged by size:
3423 1893 1459 652 456 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2993 3409 4385 4840 6544 6918
Size: 1064 138 1404 416 976 455 1704 374
Cuts at: 6918 7064 387
Size: 146 1280
Fragments arranged by size:
1704 1404 1280 1064 976 455 416 374
146 138
EcoICRI GAG'CTC
Cuts at: 2010 2227 3589 2010
Size: 217 1362 6378
Fragments arranged by size:
6378 1362 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 2453 3131 3284 3494 3757 3815
Size: 393 794 27 678 153 210 263 58
Cuts at: 3815 4013 4148 4394 4720 6020 6617 7404 7672
Size: 198 135 246 326 1300 597 787 268
Cuts at: 7672 1239
Size: 1524
Fragments arranged by size:
1524 1300 794 787 678 597 393 326
268 263 246 210 198 153 135 58
27
EcoO109I rG'GnC_Cy
Cuts at: 4542 4652 4542
Size: 110 7847
EcoRI G'AATT_C
Cuts at: 5266 5644 5266
Size: 378 7579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4399 4399
Size: 7957
FspI TGC'GCA
Cuts at: 6463 6463
Size: 7957
HaeII r_GCGC'y
Cuts at: 1678 2117 2237 2561 2889 3748 5092 7104 1678
Size: 439 120 324 328 859 1344 2012 2531
Fragments arranged by size:
2531 2012 1344 859 439 328 324 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3335 3367 5122 5154 6598
Size: 32 135 32 1763 32 1755 32 1444
Cuts at: 6598 6630 6672 6704 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1763 1755 1444 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 4675 5301 5547 669
Size: 988 281 732 2005 626 246 3079
Fragments arranged by size:
3079 2005 988 732 626 281 246
HindIII A'AGCT_T
Cuts at: 748 4472 748
Size: 3724 4233
HpaI GTT'AAC
Cuts at: 5547 5547
Size: 7957
KpnI G_GTAC'C
Cuts at: 4870 5282 4870
Size: 412 7545
MfeI C'AATT_G
Cuts at: 5556 5556
Size: 7957
MluI A'CGCG_T
Cuts at: 7833 7833
Size: 7957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2838 7071 7255 1305
Size: 1533 4233 184 2007
Fragments arranged by size:
4233 2007 1533 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 2363 2504 4361 10
Size: 54 1425 343 276 255 141 1857 3606
Fragments arranged by size:
3606 1857 1425 343 276 255 141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2025 2513 3857 3986 4430 5177 5915
Size: 648 859 488 1344 129 444 747 738
Cuts at: 5915 6274 6433 518
Size: 359 159 2042
Fragments arranged by size:
2042 1344 859 747 738 648 488 444
359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2215 2398 3412 3805 3817 4110 4626 4882
Size: 609 183 1014 393 12 293 516 256
Cuts at: 4882 5117 5373 6050 7198 7443 1606
Size: 235 256 677 1148 245 2120
Fragments arranged by size:
2120 1148 1014 677 609 516 393 293
256 256 245 235 183 12
NaeI GCC'GGC
Cuts at: 2196 3276 3667 3850 2196
Size: 1080 391 183 6303
Fragments arranged by size:
6303 1080 391 183
NcoI C'CATG_G
Cuts at: 513 1065 2148 3837 4431 4531 4707 5052 5178
Size: 552 1083 1689 594 100 176 345 126
Cuts at: 5178 5235 513
Size: 57 3235
Fragments arranged by size:
3235 1689 1083 594 552 345 176 126
100 57
NdeI CA'TA_TG
Cuts at: 387 4286 387
Size: 3899 4058
NgoMIV G'CCGG_C
Cuts at: 2194 3274 3665 3848 2194
Size: 1080 391 183 6303
Fragments arranged by size:
6303 1080 391 183
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7957
NotI GC'GGCC_GC
Cuts at: 5404 5404
Size: 7957
NspI r_CATG'y
Cuts at: 1132 1229 2089 4585 4968 5315 6860 1132
Size: 97 860 2496 383 347 1545 2229
Fragments arranged by size:
2496 2229 1545 860 383 347 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7957
PciI A'CATG_T
Cuts at: 4581 6856 4581
Size: 2275 5682
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2053 1108
Size: 945 7012
PfoI T'CCnGG_A
Cuts at: 2924 2924
Size: 7957
PmeI GTTT'AAAC
Cuts at: 5261 5261
Size: 7957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6026 6058 7539 7571 6026
Size: 32 1481 32 6412
Fragments arranged by size:
6412 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4542 4542
Size: 7957
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7957
PsiI TTA'TAA
Cuts at: 5527 5527
Size: 7957
PspOMI G'GGCC_C
Cuts at: 4652 4652
Size: 7957
PspXI vC'TCGA_Gb
Cuts at: 4919 4919
Size: 7957
PstI C_TGCA'G
Cuts at: 830 2220 2463 3249 3438 3513 4074 4482 5100
Size: 1390 243 786 189 75 561 408 618
Cuts at: 5100 5309 830
Size: 209 3478
Fragments arranged by size:
3478 1390 786 618 561 408 243 209
189 75
PvuI CG_AT'CG
Cuts at: 2001 4758 2001
Size: 2757 5200
PvuII CAG'CTG
Cuts at: 2215 3817 4882 2215
Size: 1602 1065 5290
Fragments arranged by size:
5290 1602 1065
SacI G_AGCT'C
Cuts at: 2012 2229 3591 2012
Size: 217 1362 6378
Fragments arranged by size:
6378 1362 217
SacII CC_GC'GG
Cuts at: 4111 4111
Size: 7957
SalI G'TCGA_C
Cuts at: 1936 2668 5299 1936
Size: 732 2631 4594
Fragments arranged by size:
4594 2631 732
SanDI GG'GwC_CC
Cuts at: 4542 4542
Size: 7957
SapI GCTCTTCn'nnn_
Cuts at: 3776 3776
Size: 7957
SbfI CC_TGCA'GG
Cuts at: 4074 5309 4074
Size: 1235 6722
ScaI AGT'ACT
Cuts at: 4724 5084 6205 7917 4724
Size: 360 1121 1712 4764
Fragments arranged by size:
4764 1712 1121 360
SexAI A'CCwGG_T
Cuts at: 3441 3441
Size: 7957
SfcI C'TryA_G
Cuts at: 826 1045 2216 2459 3245 3434 3509 4070 4136
Size: 219 1171 243 786 189 75 561 66
Cuts at: 4136 4478 4558 5096 5305 6440 7121 7312 826
Size: 342 80 538 209 1135 681 191 1471
Fragments arranged by size:
1471 1171 1135 786 681 561 538 342
243 219 209 191 189 80 75 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7957
SgrAI Cr'CCGG_yG
Cuts at: 1700 4688 1700
Size: 2988 4969
SmaI CCC'GGG
Cuts at: 1872 3890 5284 1872
Size: 2018 1394 4545
Fragments arranged by size:
4545 2018 1394
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2237 2468 4919 6061 6962 7224
Size: 197 926 294 231 2451 1142 901 262
Cuts at: 7224 820
Size: 1553
Fragments arranged by size:
2451 1553 1142 926 901 294 262 231
197
SnaBI TAC'GTA
Cuts at: 493 5748 493
Size: 5255 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7957
SphI G_CATG'C
Cuts at: 1132 5315 1132
Size: 4183 3774
SspI AAT'ATT
Cuts at: 5 52 5881 5
Size: 47 5829 2081
Fragments arranged by size:
5829 2081 47
StuI AGG'CCT
Cuts at: 1517 3300 3880 3969 1517
Size: 1783 580 89 5505
Fragments arranged by size:
5505 1783 580 89
StyI C'CwwG_G
Cuts at: 513 1065 2148 3207 3330 3837 4431 4531 4707
Size: 552 1083 1059 123 507 594 100 176
Cuts at: 4707 5052 5178 5235 5396 513
Size: 345 126 57 161 3074
Fragments arranged by size:
3074 1083 1059 594 552 507 345 176
161 126 123 100 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2926 3191 5984 6001 6154 6339 6784
Size: 1255 1346 265 2793 17 153 185 445
Cuts at: 6784 325
Size: 1498
Fragments arranged by size:
2793 1498 1346 1255 445 265 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4578 4722 5082
Size: 274 80 33 51 1443 2601 144 360
Cuts at: 5082 6203 7915 96
Size: 1121 1712 138
Fragments arranged by size:
2601 1712 1443 1121 360 274 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2573 2605 3300 3311 3332 3343 7917 7949 2573
Size: 32 695 11 21 11 4574 32 2581
Fragments arranged by size:
4574 2581 695 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 3400 1316
Size: 2084 5873
XbaI T'CTAG_A
Cuts at: 5293 5293
Size: 7957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3067
Size: 7957
XhoI C'TCGA_G
Cuts at: 1943 4919 1943
Size: 2976 4981
XmaI C'CCGG_G
Cuts at: 1870 3888 5282 1870
Size: 2018 1394 4545
Fragments arranged by size:
4545 2018 1394
XmnI GAAnn'nnTTC
Cuts at: 4613 6086 4613
Size: 1473 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5765 276
Size: 53 83 186 936 4231 2468
Fragments arranged by size:
4231 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PsrI RsrII SfiI SfoI
SrfI SwaI