Restriction map for
pFN21AA0284
(Circular) (Six-base) MAPSORT of: pFN21AA0284.seq Check: 4882 from: 1 to: 9262
pFN21AA0284 9262 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2143 2546 2932 2143
Size: 403 386 8473
Fragments arranged by size:
8473 403 386
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7072 278
Size: 53 83 186 936 5536 2468
Fragments arranged by size:
5536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6605 1937
Size: 4668 4594
Acc65I G'GTAC_C
Cuts at: 6583 6583
Size: 9262
AclI AA'CG_TT
Cuts at: 1563 7389 7762 1563
Size: 5826 373 3063
Fragments arranged by size:
5826 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 2861 5181 5479 6269 6302 7325 8709
Size: 425 804 2320 298 790 33 1023 1384
Cuts at: 8709 8977 1632
Size: 268 1917
Fragments arranged by size:
2320 1917 1384 1023 804 790 425 298
268 33
AfeI AGC'GCT
Cuts at: 3047 3047
Size: 9262
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9065
AflIII A'CryG_T
Cuts at: 1203 2076 5565 8161 9138 1203
Size: 873 3489 2596 977 1327
Fragments arranged by size:
3489 2596 1327 977 873
AgeI A'CCGG_T
Cuts at: 8093 8093
Size: 9262
AhdI GACnn_n'nnGTC
Cuts at: 4642 7991 4642
Size: 3349 5913
AleI CACnn'nnGTG
Cuts at: 1166 3533 1166
Size: 2367 6895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3711 1184
Size: 2527 6735
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6098 6130 6098
Size: 32 9230
AlwNI CAG_nnn'CTG
Cuts at: 3316 4477 4729 5204 5267 5363 5791 7005 8577
Size: 1161 252 475 63 96 428 1214 1572
Cuts at: 8577 3316
Size: 4001
Fragments arranged by size:
4001 1572 1214 1161 475 428 252 96
63
ApaI G_GGCC'C
Cuts at: 3413 3938 4125 4159 4707 5182 5506 5689 3413
Size: 525 187 34 548 475 324 183 6986
Fragments arranged by size:
6986 548 525 475 324 187 183 34
ApaLI G'TGCA_C
Cuts at: 7319 8475 7319
Size: 1156 8106
ApoI r'AATT_y
Cuts at: 1493 6481 6571 6797 6949 9058 1493
Size: 4988 90 226 152 2109 1697
Fragments arranged by size:
4988 2109 1697 226 152 90
AseI AT'TA_AT
Cuts at: 160 1032 7816 160
Size: 872 6784 1606
Fragments arranged by size:
6784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9262
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 2686 2761 3169 3350 3817 4070
Size: 73 570 173 75 408 181 467 253
Cuts at: 4070 4160 4488 4621 4777 4905 5067 5351 5509
Size: 90 328 133 156 128 162 284 158
Cuts at: 5509 5650 5682 5697 5928 5954 6003 6587 1870
Size: 141 32 15 231 26 49 584 4545
Fragments arranged by size:
4545 584 570 467 408 328 284 253
231 181 173 162 158 156 141 133
128 90 75 73 49 32 26 15
AvrII C'CTAG_G
Cuts at: 4258 4258
Size: 9262
BamHI G'GATC_C
Cuts at: 4021 6592 6959 4021
Size: 2571 367 6324
Fragments arranged by size:
6324 2571 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2173 2518 2917 3276 3748
Size: 325 217 627 386 345 399 359 472
Cuts at: 3748 4784 5541 5980 6238 6583 8038 618
Size: 1036 757 439 258 345 1455 1842
Fragments arranged by size:
1842 1455 1036 757 627 472 439 399
386 359 345 345 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2364 2409 2463 3329 3413 3758 3938 4112
Size: 973 45 54 866 84 345 180 174
Cuts at: 4112 4125 4159 4707 5182 5250 5506 5558 5677
Size: 13 34 548 475 68 256 52 119
Cuts at: 5677 5689 5861 6431 6581 1391
Size: 12 172 570 150 4072
Fragments arranged by size:
4072 973 866 570 548 475 345 256
180 174 172 150 119 84 68 54
52 45 34 13 12
BbeI G_GCGC'C
Cuts at: 2177 3280 5545 2177
Size: 1103 2265 5894
Fragments arranged by size:
5894 2265 1103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3212 6257 928
Size: 971 1313 3045 3933
Fragments arranged by size:
3933 3045 1313 971
BbvCI CC'TCA_GC
Cuts at: 3900 4034 5171 5888 6445 3900
Size: 134 1137 717 557 6717
Fragments arranged by size:
6717 1137 717 557 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2752 2786 4352 4386 7453
Size: 34 50 34 731 34 1566 34 3067
Cuts at: 7453 7487 1903
Size: 34 3678
Fragments arranged by size:
3678 3067 1566 731 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7154 8364 7154
Size: 1210 8052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2900 3408 3725
Size: 107 122 71 631 605 1228 508 317
Cuts at: 3725 3797 3933 4005 4559 4568 4873 5009 7873
Size: 72 136 72 554 9 305 136 2864
Cuts at: 7873 136
Size: 1525
Fragments arranged by size:
2864 1525 1228 631 605 554 508 317
305 136 136 122 107 72 72 71
9
BglII A'GATC_T
Cuts at: 9257 9257
Size: 9262
BlpI GC'TnA_GC
Cuts at: 2430 4879 5089 5268 5780 6679 2430
Size: 2449 210 179 512 899 5013
Fragments arranged by size:
5013 2449 899 512 210 179
Bme1580I G_kGCm'C
Cuts at: 1790 2922 3413 3938 4125 4159 4707 4738 4804
Size: 1132 491 525 187 34 548 31 66
Cuts at: 4804 5182 5506 5521 5689 6243 7323 8479 1790
Size: 378 324 15 168 554 1080 1156 2573
Fragments arranged by size:
2573 1156 1132 1080 554 548 525 491
378 324 187 168 66 34 31 15
BmgBI CAC'GTC
Cuts at: 1318 4660 5568 5803 1318
Size: 3342 908 235 4777
Fragments arranged by size:
4777 3342 908 235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3046 3126 3140
Size: 416 53 83 399 24 1629 80 14
Cuts at: 3140 3304 3617 3719 3903 4920 5864 6333 6993
Size: 164 313 102 184 1017 944 469 660
Cuts at: 6993 7951 442
Size: 958 1753
Fragments arranged by size:
1753 1629 1017 958 944 660 469 416
399 313 184 164 102 83 80 53
24 14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 3715 4831 5608 2290
Size: 1425 1116 777 5944
Fragments arranged by size:
5944 1425 1116 777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 3580 4224 4438 4586 5431 5650 7922
Size: 869 1472 644 214 148 845 219 2272
Cuts at: 7922 1239
Size: 2579
Fragments arranged by size:
2579 2272 1472 869 845 644 219 214
148
Bpu10I CC'TnA_GC
Cuts at: 3900 4034 5171 5244 5888 6445 3900
Size: 134 1137 73 644 557 6717
Fragments arranged by size:
6717 1137 644 557 134 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 3179 7387 8252 8550 2108
Size: 1071 4208 865 298 2820
Fragments arranged by size:
4208 2820 1071 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2248 2483 2720 3506 7925 882
Size: 617 749 235 237 786 4419 2219
Fragments arranged by size:
4419 2219 786 749 617 237 235
BsaAI yAC'GTr
Cuts at: 493 1204 4128 7053 493
Size: 711 2924 2925 2702
Fragments arranged by size:
2925 2924 2702 711
BsaBI GATnn'nnATC
Cuts at: 2639 6170 9256 2639
Size: 3531 3086 2645
Fragments arranged by size:
3531 3086 2645
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2174 2343 3277 4299
Size: 53 83 186 936 641 169 934 1022
Cuts at: 4299 4638 5542 6042 7069 7451 8086 275
Size: 339 904 500 1027 382 635 1451
Fragments arranged by size:
1451 1027 1022 936 934 904 641 635
500 382 339 186 169 83 53
BsaWI w'CCGG_w
Cuts at: 2250 3364 3970 7695 8093 8367 8514 8925 2250
Size: 1114 606 3725 398 274 147 411 2587
Fragments arranged by size:
3725 2587 1114 606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4123 4153 4496 4526 4854
Size: 30 165 30 2720 30 343 30 328
Cuts at: 4854 4884 5606 5636 5867 5897 1178
Size: 30 722 30 231 30 4543
Fragments arranged by size:
4543 2720 722 343 328 231 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2197 3485 3554 3967 4028 4568 4946
Size: 943 68 1288 69 413 61 540 378
Cuts at: 4946 5087 5594 5597 5732 5735 5851 5948 6092
Size: 141 507 3 135 3 116 97 144
Cuts at: 6092 1186
Size: 4356
Fragments arranged by size:
4356 1288 943 540 507 413 378 144
141 135 116 97 69 68 61 3
3
BseYI C'CCAG_C
Cuts at: 2346 2462 2912 3139 3220 3344 3444 3669 3786
Size: 116 450 227 81 124 100 225 117
Cuts at: 3786 3992 4272 4277 4285 4375 4426 4706 4722
Size: 206 280 5 8 90 51 280 16
Cuts at: 4722 4961 5059 5185 5837 5900 6117 6400 6512
Size: 239 98 126 652 63 217 283 112
Cuts at: 6512 8465 8961 2346
Size: 1953 496 2647
Fragments arranged by size:
2647 1953 652 496 450 283 280 280
239 227 225 217 206 126 124 117
116 112 100 98 90 81 63 51
16 8 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 2411 2603 2756 2771 4752 5768
Size: 573 381 294 192 153 15 1981 1016
Cuts at: 5768 5963 6320 6372 1163
Size: 195 357 52 4053
Fragments arranged by size:
4053 1981 1016 573 381 357 294 195
192 153 52 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2910 3117 3336 6712 7473 8501
Size: 275 186 909 207 219 3376 761 1028
Cuts at: 8501 1540
Size: 2301
Fragments arranged by size:
3376 2301 1028 909 761 275 219 207
186
BsiHKAI G_wGCw'C
Cuts at: 2409 3329 3735 6153 7323 7408 8479 2409
Size: 920 406 2418 1170 85 1071 3192
Fragments arranged by size:
3192 2418 1170 1071 920 406 85
BsmI GAATG_Cn'
Cuts at: 6773 6866 6773
Size: 93 9169
BsmBI CGTCTCn'nnnn_
Cuts at: 4535 4541 4535
Size: 6 9256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2409 2463 2922 3329 3413 3735
Size: 399 574 45 54 459 407 84 322
Cuts at: 3735 3758 3938 4112 4125 4159 4707 4738 4804
Size: 23 180 174 13 34 548 31 66
Cuts at: 4804 5182 5250 5506 5521 5558 5677 5689 5861
Size: 378 68 256 15 37 119 12 172
Cuts at: 5861 6153 6243 6431 6581 7323 7408 8479 1391
Size: 292 90 188 150 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 574 548 459 407 399
378 322 292 256 188 180 174 172
150 119 90 85 84 68 66 54
45 37 34 31 23 15 13 12
BspEI T'CCGG_A
Cuts at: 8925 8925
Size: 9262
BspHI T'CATG_A
Cuts at: 7151 8881 7151
Size: 1730 7532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2143 2546 2826 2932 6363 6617 844
Size: 1299 403 280 106 3431 254 3489
Fragments arranged by size:
3489 3431 1299 403 280 254 106
BsrBI CCG'CTC
Cuts at: 1394 1823 2570 3282 3377 4701 5127 5224 5508
Size: 429 747 712 95 1324 426 97 284
Cuts at: 5508 7149 1394
Size: 1641 3507
Fragments arranged by size:
3507 1641 1324 747 712 429 426 284
97 95
BsrDI GCAATG_nn'
Cuts at: 65 1248 7757 7931 65
Size: 1183 6509 174 1396
Fragments arranged by size:
6509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2521 3112 3185 4867 5256 5324 5544
Size: 110 711 591 73 1682 389 68 220
Cuts at: 5544 7906 8093 1700
Size: 2362 187 2869
Fragments arranged by size:
2869 2362 1682 711 591 389 220 187
110 73 68
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9262
BssSI C'ACGA_G
Cuts at: 2528 2855 5536 7322 8334 2528
Size: 327 2681 1786 1012 3456
Fragments arranged by size:
3456 2681 1786 1012 327
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5363 5762 794
Size: 4569 399 4294
Fragments arranged by size:
4569 4294 399
BstBI TT'CG_AA
Cuts at: 8066 8066
Size: 9262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9262
BstXI CCAn_nnnn'nTGG
Cuts at: 3406 3569 3406
Size: 163 9099
BstYI r'GATC_y
Cuts at: 1972 2475 4021 5823 6171 6592 6959 7344 7361
Size: 503 1546 1802 348 421 367 385 17
Cuts at: 7361 8813 8899 8911 9257 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1802 1546 1452 503 421 385 367
348 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2436 4998 6476 2436
Size: 2562 1478 5222
Fragments arranged by size:
5222 2562 1478
BtgI C'CryG_G
Cuts at: 513 1065 2410 3100 3391 3405 3435 4858 5250
Size: 552 1345 690 291 14 30 1423 392
Cuts at: 5250 5424 5475 5599 513
Size: 174 51 124 4176
Fragments arranged by size:
4176 1423 1345 690 552 392 291 174
124 51 30 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4053 487
Size: 683 56 2827 5696
Fragments arranged by size:
5696 2827 683 56
BtsI GCAGTG_nn'
Cuts at: 2192 5395 5521 6778 7572 7592 2192
Size: 3203 126 1257 794 20 3862
Fragments arranged by size:
3862 3203 1257 794 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3082 3117 529
Size: 35 2518 35 6674
Fragments arranged by size:
6674 2518 35 35
DraI TTT'AAA
Cuts at: 6566 6566
Size: 9262
DraIII CAC_nnn'GTG
Cuts at: 3713 5833 3713
Size: 2120 7142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2822 8269 809
Size: 507 1506 5447 1802
Fragments arranged by size:
5447 1802 1506 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2736 2892 2907
Size: 54 1425 50 275 18 906 156 15
Cuts at: 2907 3114 3333 3609 3704 4006 4479 4894 5258
Size: 207 219 276 95 302 473 415 364
Cuts at: 5258 5304 5383 5427 5805 6048 6378 6709 7598
Size: 46 79 44 378 243 330 331 889
Cuts at: 7598 9227 8
Size: 1629 43
Fragments arranged by size:
1629 1425 906 889 473 415 378 364
331 330 302 276 275 243 219 207
156 95 79 54 50 46 44 43
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2907 3114 3333 6709 1537
Size: 275 1095 207 219 3376 4090
Fragments arranged by size:
4090 3376 1095 275 219 207
EarI CTCTTCn'nnn_
Cuts at: 1353 4007 7192 1353
Size: 2654 3185 3423
Fragments arranged by size:
3423 3185 2654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2580 3743 7849 8223 8369 387
Size: 1064 138 991 1163 4106 374 146 1280
Fragments arranged by size:
4106 1280 1163 1064 991 374 146 138
EcoICRI GAG'CTC
Cuts at: 2407 3327 2407
Size: 920 8342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2108 2861 3580 4224 4438 4586
Size: 393 425 51 753 719 644 214 148
Cuts at: 4586 5181 5431 5479 5650 6269 6302 7325 7922
Size: 595 250 48 171 619 33 1023 597
Cuts at: 7922 8709 8977 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1023 787 753 719 644 619 597
595 425 393 268 250 214 171 148
51 48 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2894 3293 3827 4819 5497 5816 2894
Size: 399 534 992 678 319 6340
Fragments arranged by size:
6340 992 678 534 399 319
EcoO109I rG'GnC_Cy
Cuts at: 2384 2884 2971 3079 3410 3739 3867 4067 4121
Size: 500 87 108 331 329 128 200 54
Cuts at: 4121 4155 4156 4255 4474 4774 4902 4911 5229
Size: 34 1 99 219 300 128 9 318
Cuts at: 5229 5480 5502 5686 6205 6454 2384
Size: 251 22 184 519 249 5192
Fragments arranged by size:
5192 519 500 331 329 318 300 251
249 219 200 184 128 128 108 99
87 54 34 22 9 1
EcoRI G'AATT_C
Cuts at: 6571 6949 6571
Size: 378 8884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6193 6193
Size: 9262
FseI GG_CCGG'CC
Cuts at: 5260 5260
Size: 9262
FspI TGC'GCA
Cuts at: 2009 3881 7768 2009
Size: 1872 3887 3503
Fragments arranged by size:
3887 3503 1872
FspAI rTGC'GCAy
Cuts at: 2009 3881 2009
Size: 1872 7390
HaeII r_GCGC'y
Cuts at: 1678 2177 2353 2377 3049 3100 3280 3683 4147
Size: 499 176 24 672 51 180 403 464
Cuts at: 4147 4433 4879 5545 5883 8409 1678
Size: 286 446 666 338 2526 2531
Fragments arranged by size:
2531 2526 672 666 499 464 446 403
338 286 180 176 51 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2702 2734 3794 3826 4123
Size: 32 135 32 1130 32 1060 32 297
Cuts at: 4123 4155 4496 4528 4628 4660 4813 4845 5213
Size: 32 341 32 100 32 153 32 368
Cuts at: 5213 5245 5552 5584 6049 6081 7903 7935 7977
Size: 32 307 32 465 32 1822 32 42
Cuts at: 7977 8009 1373
Size: 32 2626
Fragments arranged by size:
2626 1822 1130 1060 465 368 341 307
297 153 135 100 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6606 6852 669
Size: 988 281 4668 246 3079
Fragments arranged by size:
4668 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9262
HpaI GTT'AAC
Cuts at: 6852 6852
Size: 9262
KasI G'GCGC_C
Cuts at: 2173 3276 5541 2173
Size: 1103 2265 5894
Fragments arranged by size:
5894 2265 1103
KpnI G_GTAC'C
Cuts at: 6587 6587
Size: 9262
MfeI C'AATT_G
Cuts at: 6861 6861
Size: 9262
MluI A'CGCG_T
Cuts at: 9138 9138
Size: 9262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 3558 3999 4032 5007 5154 5556 8376
Size: 792 1461 441 33 975 147 402 2820
Cuts at: 8376 8560 1305
Size: 184 2007
Fragments arranged by size:
2820 2007 1461 975 792 441 402 184
147 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2894 4896 5306 5807 6050
Size: 54 1425 343 1062 2002 410 501 243
Cuts at: 6050 6380 10
Size: 330 2892
Fragments arranged by size:
2892 2002 1425 1062 501 410 343 330
243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 3533 7220 7579 7738 518
Size: 648 915 1452 3687 359 159 2042
Fragments arranged by size:
3687 2042 1452 915 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 2677 2912 2934 3448 3673 3786 3904
Size: 543 528 235 22 514 225 113 118
Cuts at: 3904 4766 5042 5063 5264 6033 6121 6310 6493
Size: 862 276 21 201 769 88 189 183
Cuts at: 6493 6501 6678 7355 8503 8748 1606
Size: 8 177 677 1148 245 2120
Fragments arranged by size:
2120 1148 862 769 677 543 528 514
276 245 235 225 201 189 183 177
118 113 88 22 21 8
NaeI GCC'GGC
Cuts at: 2523 3114 3187 5258 5326 5546 2523
Size: 591 73 2071 68 220 6239
Fragments arranged by size:
6239 2071 591 220 73 68
NarI GG'CG_CC
Cuts at: 2174 3277 5542 2174
Size: 1103 2265 5894
Fragments arranged by size:
5894 2265 1103
NcoI C'CATG_G
Cuts at: 513 1065 3391 5424 513
Size: 552 2326 2033 4351
Fragments arranged by size:
4351 2326 2033 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9262
NgoMIV G'CCGG_C
Cuts at: 2521 3112 3185 5256 5324 5544 2521
Size: 591 73 2071 68 220 6239
Fragments arranged by size:
6239 2071 591 220 73 68
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9262
NotI GC'GGCC_GC
Cuts at: 6709 6709
Size: 9262
NspI r_CATG'y
Cuts at: 1132 1229 2080 3886 5899 6620 8165 1132
Size: 97 851 1806 2013 721 1545 2229
Fragments arranged by size:
2229 2013 1806 1545 851 721 97
PasI CC'CwG_GG
Cuts at: 1348 2786 3761 5233 1348
Size: 1438 975 1472 5377
Fragments arranged by size:
5377 1472 1438 975
PciI A'CATG_T
Cuts at: 2076 8161 2076
Size: 6085 3177
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3676 4247 4418 5791 5833 1108
Size: 2568 571 171 1373 42 4537
Fragments arranged by size:
4537 2568 1373 571 171 42
PfoI T'CCnGG_A
Cuts at: 2013 2109 5447 6519 2013
Size: 96 3338 1072 4756
Fragments arranged by size:
4756 3338 1072 96
PmeI GTTT'AAAC
Cuts at: 6566 6566
Size: 9262
PmlI CAC'GTG
Cuts at: 4128 4128
Size: 9262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7331 7363 8844 8876 7331
Size: 32 1481 32 7717
Fragments arranged by size:
7717 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2384 2884 2971 3079 3867 4474 4774 4911 5229
Size: 500 87 108 788 607 300 137 318
Cuts at: 5229 5480 6205 2384
Size: 251 725 5441
Fragments arranged by size:
5441 788 725 607 500 318 300 251
137 108 87
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9262
PsiI TTA'TAA
Cuts at: 6832 6832
Size: 9262
PspOMI G'GGCC_C
Cuts at: 3409 3934 4121 4155 4703 5178 5502 5685 3409
Size: 525 187 34 548 475 324 183 6986
Fragments arranged by size:
6986 548 525 475 324 187 183 34
PstI C_TGCA'G
Cuts at: 830 2154 2624 2955 3690 6614 830
Size: 1324 470 331 735 2924 3478
Fragments arranged by size:
3478 2924 1324 735 470 331
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9262
PvuII CAG'CTG
Cuts at: 2149 3448 3904 4766 5063 5264 6033 6121 6310
Size: 1299 456 862 297 201 769 88 189
Cuts at: 6310 6501 2149
Size: 191 4910
Fragments arranged by size:
4910 1299 862 769 456 297 201 191
189 88
SacI G_AGCT'C
Cuts at: 2409 3329 2409
Size: 920 8342
SalI G'TCGA_C
Cuts at: 1936 6604 1936
Size: 4668 4594
SanDI GG'GwC_CC
Cuts at: 2384 2971 3079 3867 4911 5229 5480 2384
Size: 587 108 788 1044 318 251 6166
Fragments arranged by size:
6166 1044 788 587 318 251 108
SbfI CC_TGCA'GG
Cuts at: 6614 6614
Size: 9262
ScaI AGT'ACT
Cuts at: 7510 9222 7510
Size: 1712 7550
SexAI A'CCwGG_T
Cuts at: 3561 3561
Size: 9262
SfcI C'TryA_G
Cuts at: 826 1045 2150 2620 2951 3686 4469 6610 7745
Size: 219 1105 470 331 735 783 2141 1135
Cuts at: 7745 8426 8617 826
Size: 681 191 1471
Fragments arranged by size:
2141 1471 1135 1105 783 735 681 470
331 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2900 4559 4568 2900
Size: 1659 9 7594
Fragments arranged by size:
7594 1659 9
SfoI GGC'GCC
Cuts at: 2175 3278 5543 2175
Size: 1103 2265 5894
Fragments arranged by size:
5894 2265 1103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9262
SmaI CCC'GGG
Cuts at: 1872 2688 3819 4162 4490 4779 5069 5684 5699
Size: 816 1131 343 328 289 290 615 15
Cuts at: 5699 5930 6589 1872
Size: 231 659 4545
Fragments arranged by size:
4545 1131 816 659 615 343 328 290
289 231 15
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2123 3194 7366 8267 8529 820
Size: 197 926 180 1071 4172 901 262 1553
Fragments arranged by size:
4172 1553 1071 926 901 262 197 180
SnaBI TAC'GTA
Cuts at: 493 7053 493
Size: 6560 2702
SpeI A'CTAG_T
Cuts at: 152 5342 152
Size: 5190 4072
SphI G_CATG'C
Cuts at: 1132 3886 6620 1132
Size: 2754 2734 3774
Fragments arranged by size:
3774 2754 2734
SspI AAT'ATT
Cuts at: 5 52 7186 5
Size: 47 7134 2081
Fragments arranged by size:
7134 2081 47
StuI AGG'CCT
Cuts at: 1517 2222 3002 5117 1517
Size: 705 780 2115 5662
Fragments arranged by size:
5662 2115 780 705
StyI C'CwwG_G
Cuts at: 513 1065 2379 2730 2895 2922 3084 3391 3453
Size: 552 1314 351 165 27 162 307 62
Cuts at: 3453 4151 4258 5424 6701 513
Size: 698 107 1166 1277 3074
Fragments arranged by size:
3074 1314 1277 1166 698 552 351 307
165 162 107 62 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3530 3701 3766 4538 4848 7289 7306
Size: 1255 1950 171 65 772 310 2441 17
Cuts at: 7306 7459 7644 8089 325
Size: 153 185 445 1498
Fragments arranged by size:
2441 1950 1498 1255 772 445 310 185
171 153 65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 7508 9220
Size: 274 80 33 51 1443 159 5372 1712
Cuts at: 9220 96
Size: 138
Fragments arranged by size:
5372 1712 1443 274 159 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 4586 4618 5464 5496 5606 5638 5867
Size: 32 1498 32 846 32 110 32 229
Cuts at: 5867 5899 9222 9254 3056
Size: 32 3323 32 3064
Fragments arranged by size:
3323 3064 1498 846 229 110 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3563 1316
Size: 2247 7015
XbaI T'CTAG_A
Cuts at: 6598 6598
Size: 9262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4321 4340 5834 6346 4321
Size: 19 1494 512 7237
Fragments arranged by size:
7237 1494 512 19
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9262
XmaI C'CCGG_G
Cuts at: 1870 2686 3817 4160 4488 4777 5067 5682 5697
Size: 816 1131 343 328 289 290 615 15
Cuts at: 5697 5928 6587 1870
Size: 231 659 4545
Fragments arranged by size:
4545 1131 816 659 615 343 328 290
289 231 15
XmnI GAAnn'nnTTC
Cuts at: 7391 7391
Size: 9262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7070 276
Size: 53 83 186 936 5536 2468
Fragments arranged by size:
5536 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I EcoRV NruI NsiI
PacI PspXI PsrI RsrII SacII SapI SrfI SwaI