Restriction map for
pFN21AA0266
(Circular) (Six-base) MAPSORT of: pFN21AA0266.seq Check: 7706 from: 1 to: 7003
pFN21AA0266 7003 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4813 278
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4346 1937
Size: 2409 4594
Acc65I G'GTAC_C
Cuts at: 4324 4324
Size: 7003
AclI AA'CG_TT
Cuts at: 1563 5130 5503 1563
Size: 3567 373 3063
Fragments arranged by size:
3567 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 2468 2666 5066 6450 6718 1632
Size: 624 212 198 2400 1384 268 1917
Fragments arranged by size:
2400 1917 1384 624 268 212 198
AflII C'TTAA_G
Cuts at: 820 1017 2891 820
Size: 197 1874 4932
Fragments arranged by size:
4932 1874 197
AflIII A'CryG_T
Cuts at: 1203 4277 5902 6879 1203
Size: 3074 1625 977 1327
Fragments arranged by size:
3074 1625 1327 977
AgeI A'CCGG_T
Cuts at: 5834 5834
Size: 7003
AhdI GACnn_n'nnGTC
Cuts at: 5732 5732
Size: 7003
AleI CACnn'nnGTG
Cuts at: 1166 3053 1166
Size: 1887 5116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7003
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3054 3086 3054
Size: 32 6971
AlwNI CAG_nnn'CTG
Cuts at: 2532 3135 4746 6318 2532
Size: 603 1611 1572 3217
Fragments arranged by size:
3217 1611 1572 603
ApaLI G'TGCA_C
Cuts at: 5060 6216 5060
Size: 1156 5847
ApoI r'AATT_y
Cuts at: 1493 2562 3252 3266 3988 4312 4538 4690 6799
Size: 1069 690 14 722 324 226 152 2109
Cuts at: 6799 1493
Size: 1697
Fragments arranged by size:
2109 1697 1069 722 690 324 226 152
14
AseI AT'TA_AT
Cuts at: 160 1032 5557 160
Size: 872 4525 1606
Fragments arranged by size:
4525 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
AvaI C'yCGr_G
Cuts at: 1870 1943 2691 4328 1870
Size: 73 748 1637 4545
Fragments arranged by size:
4545 1637 748 73
BamHI G'GATC_C
Cuts at: 4112 4333 4700 4112
Size: 221 367 6415
Fragments arranged by size:
6415 367 221
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4324 5779 618
Size: 325 217 627 2537 1455 1842
Fragments arranged by size:
2537 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2280 2692 2698 3306 4322 1391
Size: 889 412 6 608 1016 4072
Fragments arranged by size:
4072 1016 889 608 412 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4215 928
Size: 971 2316 3716
Fragments arranged by size:
3716 2316 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3073 3107 5194 5228 1903
Size: 34 1136 34 2087 34 3678
Fragments arranged by size:
3678 2087 1136 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4895 6105 4895
Size: 1210 5793
BclI T'GATC_A
Cuts at: 1548 3672 1548
Size: 2124 4879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5614 136
Size: 107 122 71 631 605 3942 1525
Fragments arranged by size:
3942 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2266 3296 6998 2266
Size: 1030 3702 2271
Fragments arranged by size:
3702 2271 1030
BlpI GC'TnA_GC
Cuts at: 3318 4420 3318
Size: 1102 5901
Bme1580I G_kGCm'C
Cuts at: 1790 3385 5064 6220 1790
Size: 1595 1679 1156 2573
Fragments arranged by size:
2573 1679 1595 1156
BmgBI CAC'GTC
Cuts at: 1318 3871 4280 1318
Size: 2553 409 4041
Fragments arranged by size:
4041 2553 409
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4734 5692
Size: 416 53 83 399 24 2501 816 958
Cuts at: 5692 442
Size: 1753
Fragments arranged by size:
2501 1753 958 816 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2573 2972 2996 5663 1239
Size: 1334 399 24 2667 2579
Fragments arranged by size:
2667 2579 1334 399 24
Bpu10I CC'TnA_GC
Cuts at: 3177 3177
Size: 7003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2104 2294 4213 5128 5993 6291 2104
Size: 190 1919 915 865 298 2816
Fragments arranged by size:
2816 1919 915 865 298 190
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3762 5666 882
Size: 617 2263 1904 2219
Fragments arranged by size:
2263 2219 1904 617
BsaAI yAC'GTr
Cuts at: 493 1204 4794 493
Size: 711 3590 2702
Fragments arranged by size:
3590 2702 711
BsaBI GATnn'nnATC
Cuts at: 6997 6997
Size: 7003
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3940 4810 5192 5827
Size: 53 83 186 936 2407 870 382 635
Cuts at: 5827 275
Size: 1451
Fragments arranged by size:
2407 1451 936 870 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5436 5834 6108 6255 6666 5436
Size: 398 274 147 411 5773
Fragments arranged by size:
5773 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2994 3024 3353 3383 3688
Size: 30 165 30 1591 30 329 30 305
Cuts at: 3688 3718 1178
Size: 30 4463
Fragments arranged by size:
4463 1591 329 305 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 3053 3758 3776 1186
Size: 1378 489 705 18 4413
Fragments arranged by size:
4413 1378 705 489 18
BseYI C'CCAG_C
Cuts at: 2254 3547 3800 3891 6206 6702 2254
Size: 1293 253 91 2315 496 2555
Fragments arranged by size:
2555 2315 1293 496 253 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3113 3121 1163
Size: 573 1377 8 5045
Fragments arranged by size:
5045 1377 573 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4453 5214 6242 1540
Size: 275 186 2452 761 1028 2301
Fragments arranged by size:
2452 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3306 5064 5149 6220 3306
Size: 1758 85 1071 4089
Fragments arranged by size:
4089 1758 1071 85
BsmI GAATG_Cn'
Cuts at: 2399 4514 4607 2399
Size: 2115 93 4795
Fragments arranged by size:
4795 2115 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2692 2698 3306 3385 4322 5064
Size: 399 490 412 6 608 79 937 742
Cuts at: 5064 5149 6220 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 937 742 608 490 412 399
85 79 6
BspEI T'CCGG_A
Cuts at: 6666 6666
Size: 7003
BspHI T'CATG_A
Cuts at: 3202 4892 6622 3202
Size: 1690 1730 3583
Fragments arranged by size:
3583 1730 1690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3481 4248 4358 844
Size: 2637 767 110 3489
Fragments arranged by size:
3489 2637 767 110
BsrBI CCG'CTC
Cuts at: 1394 1823 4890 1394
Size: 429 3067 3507
Fragments arranged by size:
3507 3067 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2502 5498 5672 65
Size: 1183 1254 2996 174 1396
Fragments arranged by size:
2996 1396 1254 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5647 5834 1700
Size: 110 3837 187 2869
Fragments arranged by size:
3837 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7003
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7003
BssSI C'ACGA_G
Cuts at: 5063 6075 5063
Size: 1012 5991
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7003
BstBI TT'CG_AA
Cuts at: 5807 5807
Size: 7003
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7003
BstXI CCAn_nnnn'nTGG
Cuts at: 2650 2875 2906 2650
Size: 225 31 6747
Fragments arranged by size:
6747 225 31
BstYI r'GATC_y
Cuts at: 1972 2266 2382 2602 3296 4112 4333 4700 5085
Size: 294 116 220 694 816 221 367 385
Cuts at: 5085 5102 6554 6640 6652 6998 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 816 694 385 367 346 294
221 220 116 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3645 3963 3645
Size: 318 6685
BtgI C'CryG_G
Cuts at: 513 1065 2619 513
Size: 552 1554 4897
Fragments arranged by size:
4897 1554 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6264
Fragments arranged by size:
6264 683 56
BtsI GCAGTG_nn'
Cuts at: 4519 5313 5333 4519
Size: 794 20 6189
Fragments arranged by size:
6189 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7003
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2325 2360 3852 3887 529
Size: 35 1761 35 1492 35 3645
Fragments arranged by size:
3645 1761 1492 35 35 35
DraI TTT'AAA
Cuts at: 4307 4307
Size: 7003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3700 4244 6010 809
Size: 507 2384 544 1766 1802
Fragments arranged by size:
2384 1802 1766 544 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2937 2982
Size: 54 1425 50 275 18 471 636 45
Cuts at: 2982 4450 5339 6968 8
Size: 1468 889 1629 43
Fragments arranged by size:
1629 1468 1425 889 636 471 275 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4450 1537
Size: 275 2638 4090
Fragments arranged by size:
4090 2638 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2649 3030 3528 3537 3753 3830 4933 1353
Size: 1296 381 498 9 216 77 1103 3423
Fragments arranged by size:
3423 1296 1103 498 381 216 77 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2191 5590 5964 6110 387
Size: 1064 138 602 3399 374 146 1280
Fragments arranged by size:
3399 1280 1064 602 374 146 138
EcoICRI GAG'CTC
Cuts at: 3304 3304
Size: 7003
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2468 2573 2666 2972 2996 5066
Size: 393 624 212 105 93 306 24 2070
Cuts at: 5066 5663 6450 6718 1239
Size: 597 787 268 1524
Fragments arranged by size:
2070 1524 787 624 597 393 306 268
212 105 93 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2486 2555 2654 2486
Size: 69 99 6835
Fragments arranged by size:
6835 99 69
EcoO109I rG'GnC_Cy
Cuts at: 2419 2434 3172 3618 3968 2419
Size: 15 738 446 350 5454
Fragments arranged by size:
5454 738 446 350 15
EcoRI G'AATT_C
Cuts at: 4312 4690 4312
Size: 378 6625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2809 3083 2809
Size: 274 6729
FspI TGC'GCA
Cuts at: 5509 5509
Size: 7003
HaeII r_GCGC'y
Cuts at: 1678 6150 1678
Size: 4472 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 3288 3320 3353
Size: 32 135 32 541 32 1143 32 33
Cuts at: 3353 3385 5644 5676 5718 5750 1373
Size: 32 2259 32 42 32 2626
Fragments arranged by size:
2626 2259 1143 541 135 42 33 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4347 4593 669
Size: 988 281 2409 246 3079
Fragments arranged by size:
3079 2409 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2135 3792 748
Size: 1387 1657 3959
Fragments arranged by size:
3959 1657 1387
HpaI GTT'AAC
Cuts at: 4593 4593
Size: 7003
KpnI G_GTAC'C
Cuts at: 4328 4328
Size: 7003
MfeI C'AATT_G
Cuts at: 4602 4602
Size: 7003
MluI A'CGCG_T
Cuts at: 6879 6879
Size: 7003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2852 6117 6301 1305
Size: 1547 3265 184 2007
Fragments arranged by size:
3265 2007 1547 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 2939 2984 10
Size: 54 1425 343 471 636 45 4029
Fragments arranged by size:
4029 1425 636 471 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3053 4961 5320 5479 518
Size: 648 843 1044 1908 359 159 2042
Fragments arranged by size:
2042 1908 1044 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2470 4419 5096 6244 6489 1606
Size: 864 1949 677 1148 245 2120
Fragments arranged by size:
2120 1949 1148 864 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2619 513
Size: 552 1554 4897
Fragments arranged by size:
4897 1554 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7003
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7003
NotI GC'GGCC_GC
Cuts at: 4450 4450
Size: 7003
NspI r_CATG'y
Cuts at: 1132 1229 2522 4361 5906 1132
Size: 97 1293 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 1293 97
PasI CC'CwG_GG
Cuts at: 1348 2482 1348
Size: 1134 5869
PciI A'CATG_T
Cuts at: 5902 5902
Size: 7003
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7003
PfoI T'CCnGG_A
Cuts at: 3416 3416
Size: 7003
PmeI GTTT'AAAC
Cuts at: 4307 4307
Size: 7003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2508 2540 5072 5104 6585 6617 2508
Size: 32 2532 32 1481 32 2894
Fragments arranged by size:
2894 2532 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2419 2434 3172 3618 3968 2419
Size: 15 738 446 350 5454
Fragments arranged by size:
5454 738 446 350 15
PshAI GACnn'nnGTC
Cuts at: 1613 3607 1613
Size: 1994 5009
PsiI TTA'TAA
Cuts at: 4573 4573
Size: 7003
PstI C_TGCA'G
Cuts at: 830 2703 4355 830
Size: 1873 1652 3478
Fragments arranged by size:
3478 1873 1652
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7003
PvuII CAG'CTG
Cuts at: 2470 2470
Size: 7003
RsrII CG'GwC_CG
Cuts at: 3110 3110
Size: 7003
SacI G_AGCT'C
Cuts at: 3306 3306
Size: 7003
SalI G'TCGA_C
Cuts at: 1936 4345 1936
Size: 2409 4594
SanDI GG'GwC_CC
Cuts at: 2434 2434
Size: 7003
SapI GCTCTTCn'nnn_
Cuts at: 3528 3537 3528
Size: 9 6994
SbfI CC_TGCA'GG
Cuts at: 4355 4355
Size: 7003
ScaI AGT'ACT
Cuts at: 5251 6963 5251
Size: 1712 5291
SexAI A'CCwGG_T
Cuts at: 2016 2016
Size: 7003
SfcI C'TryA_G
Cuts at: 826 1045 2699 2813 3503 3680 4107 4351 5486
Size: 219 1654 114 690 177 427 244 1135
Cuts at: 5486 6167 6358 826
Size: 681 191 1471
Fragments arranged by size:
1654 1471 1135 690 681 427 244 219
191 177 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7003
SmaI CCC'GGG
Cuts at: 1872 4330 1872
Size: 2458 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2083 2273 2891 4228 5107 6008
Size: 197 926 140 190 618 1337 879 901
Cuts at: 6008 6270 820
Size: 262 1553
Fragments arranged by size:
1553 1337 926 901 879 618 262 197
190 140
SnaBI TAC'GTA
Cuts at: 493 4794 493
Size: 4301 2702
SpeI A'CTAG_T
Cuts at: 152 2056 152
Size: 1904 5099
SphI G_CATG'C
Cuts at: 1132 4361 1132
Size: 3229 3774
SspI AAT'ATT
Cuts at: 5 52 4927 5
Size: 47 4875 2081
Fragments arranged by size:
4875 2081 47
StuI AGG'CCT
Cuts at: 1517 2630 1517
Size: 1113 5890
StyI C'CwwG_G
Cuts at: 513 1065 2619 2718 4158 4442 513
Size: 552 1554 99 1440 284 3074
Fragments arranged by size:
3074 1554 1440 552 284 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3127 4142 5030 5047 5200 5385 5830
Size: 1255 1547 1015 888 17 153 185 445
Cuts at: 5830 325
Size: 1498
Fragments arranged by size:
1547 1498 1255 1015 888 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3520 5249 6961
Size: 274 80 33 51 1443 1543 1729 1712
Cuts at: 6961 96
Size: 138
Fragments arranged by size:
1729 1712 1543 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2994 3026 3688 3720 6963 6995 2994
Size: 32 662 32 3243 32 3002
Fragments arranged by size:
3243 3002 662 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7003
XbaI T'CTAG_A
Cuts at: 4339 4339
Size: 7003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2947 2947
Size: 7003
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7003
XmaI C'CCGG_G
Cuts at: 1870 4328 1870
Size: 2458 4545
XmnI GAAnn'nnTTC
Cuts at: 5132 5132
Size: 7003
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4811 276
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI SacII SfiI SfoI SrfI
SwaI