Restriction map for
pFN21AA0261
(Circular) (Six-base) MAPSORT of: pFN21AA0261.seq Check: 1114 from: 1 to: 8530
pFN21AA0261 8530 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2139 2139
Size: 8530
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6340 278
Size: 53 83 186 936 4804 2468
Fragments arranged by size:
4804 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5873 1937
Size: 3936 4594
Acc65I G'GTAC_C
Cuts at: 5851 5851
Size: 8530
AclI AA'CG_TT
Cuts at: 1563 6657 7030 1563
Size: 5094 373 3063
Fragments arranged by size:
5094 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2574 2739 5141 5570 6593 7977 8245 1632
Size: 942 165 2402 429 1023 1384 268 1917
Fragments arranged by size:
2402 1917 1384 1023 942 429 268 165
AfeI AGC'GCT
Cuts at: 5142 5142
Size: 8530
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8333
AflIII A'CryG_T
Cuts at: 1203 4277 7429 8406 1203
Size: 3074 3152 977 1327
Fragments arranged by size:
3152 3074 1327 977
AgeI A'CCGG_T
Cuts at: 7361 7361
Size: 8530
AhdI GACnn_n'nnGTC
Cuts at: 3763 7259 3763
Size: 3496 5034
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4147 1184
Size: 2963 5567
AlwNI CAG_nnn'CTG
Cuts at: 4405 6273 7845 4405
Size: 1868 1572 5090
Fragments arranged by size:
5090 1868 1572
ApaI G_GGCC'C
Cuts at: 2018 2018
Size: 8530
ApaLI G'TGCA_C
Cuts at: 6587 7743 6587
Size: 1156 7374
ApoI r'AATT_y
Cuts at: 1493 2315 2430 2521 2770 3187 3539 4199 5741
Size: 822 115 91 249 417 352 660 1542
Cuts at: 5741 5839 6065 6217 8326 1493
Size: 98 226 152 2109 1697
Fragments arranged by size:
2109 1697 1542 822 660 417 352 249
226 152 115 98 91
AseI AT'TA_AT
Cuts at: 160 1032 2747 7084 160
Size: 872 1715 4337 1606
Fragments arranged by size:
4337 1715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8530
AvaI C'yCGr_G
Cuts at: 1870 1943 5855 1870
Size: 73 3912 4545
Fragments arranged by size:
4545 3912 73
BamHI G'GATC_C
Cuts at: 5860 6227 5860
Size: 367 8163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2058 3209 5851 7306 618
Size: 325 217 627 271 1151 2642 1455 1842
Fragments arranged by size:
2642 1842 1455 1151 627 325 271 217
BanII G_rGCy'C
Cuts at: 1391 2018 5849 1391
Size: 627 3831 4072
Fragments arranged by size:
4072 3831 627
BbeI G_GCGC'C
Cuts at: 2062 2062
Size: 8530
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 3012 4002 4149 928
Size: 971 741 372 990 147 5309
Fragments arranged by size:
5309 990 971 741 372 147
BbvCI CC'TCA_GC
Cuts at: 4962 4962
Size: 8530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6721 6755 1903
Size: 34 4784 34 3678
Fragments arranged by size:
4784 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3376 6422 7632 3376
Size: 3046 1210 4274
Fragments arranged by size:
4274 3046 1210
BclI T'GATC_A
Cuts at: 1548 5430 1548
Size: 3882 4648
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7141 136
Size: 107 122 71 631 605 5469 1525
Fragments arranged by size:
5469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4483 8525 4483
Size: 4042 4488
BlpI GC'TnA_GC
Cuts at: 4978 5947 4978
Size: 969 7561
Bme1580I G_kGCm'C
Cuts at: 1790 2018 6591 7747 1790
Size: 228 4573 1156 2573
Fragments arranged by size:
4573 2573 1156 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2893 4317 4641
Size: 416 53 83 399 24 1476 1424 324
Cuts at: 4641 6261 7219 442
Size: 1620 958 1753
Fragments arranged by size:
1753 1620 1476 1424 958 416 399 324
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4479 5408 1056
Size: 3423 929 4178
Fragments arranged by size:
4178 3423 929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2013 2487 7190 1239
Size: 774 474 4703 2579
Fragments arranged by size:
4703 2579 774 474
Bpu10I CC'TnA_GC
Cuts at: 2351 3223 4962 5212 2351
Size: 872 1739 250 5669
Fragments arranged by size:
5669 1739 872 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5402 6655 7520 7818 5402
Size: 1253 865 298 6114
Fragments arranged by size:
6114 1253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2363 5132 7193 882
Size: 617 864 2769 2061 2219
Fragments arranged by size:
2769 2219 2061 864 617
BsaAI yAC'GTr
Cuts at: 493 1204 4174 6321 493
Size: 711 2970 2147 2702
Fragments arranged by size:
2970 2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 3030 8524 3030
Size: 5494 3036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2059 6337 6719 7354
Size: 53 83 186 936 526 4278 382 635
Cuts at: 7354 275
Size: 1451
Fragments arranged by size:
4278 1451 936 635 526 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6963 7361 7635 7782 8193 6963
Size: 398 274 147 411 7300
Fragments arranged by size:
7300 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8305
Fragments arranged by size:
8305 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2250 5564 5567 1186
Size: 1064 3314 3 4149
Fragments arranged by size:
4149 3314 1064 3
BseYI C'CCAG_C
Cuts at: 4389 5206 5400 7733 8229 4389
Size: 817 194 2333 496 4690
Fragments arranged by size:
4690 2333 817 496 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4041 4107 5390 1163
Size: 573 2305 66 1283 4303
Fragments arranged by size:
4303 2305 1283 573 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5980 6741 7769 1540
Size: 275 186 3979 761 1028 2301
Fragments arranged by size:
3979 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2699 4262 4348 5248 5288 6591 6676 7747 2699
Size: 1563 86 900 40 1303 85 1071 3482
Fragments arranged by size:
3482 1563 1303 1071 900 86 85 40
BsmI GAATG_Cn'
Cuts at: 4338 6041 6134 4338
Size: 1703 93 6734
Fragments arranged by size:
6734 1703 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3455 3455
Size: 8530
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2699 4262 4348 5248 5288 5849
Size: 399 228 681 1563 86 900 40 561
Cuts at: 5849 6591 6676 7747 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1563 1071 900 742 681 561 399
228 86 85 40
BspEI T'CCGG_A
Cuts at: 8193 8193
Size: 8530
BspHI T'CATG_A
Cuts at: 2776 3559 6419 8149 2776
Size: 783 2860 1730 3157
Fragments arranged by size:
3157 2860 1730 783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 5885 844
Size: 1295 3746 3489
Fragments arranged by size:
3746 3489 1295
BsrBI CCG'CTC
Cuts at: 1394 1823 2064 2188 5392 6417 1394
Size: 429 241 124 3204 1025 3507
Fragments arranged by size:
3507 3204 1025 429 241 124
BsrDI GCAATG_nn'
Cuts at: 65 1248 3748 4368 5824 7025 7199 65
Size: 1183 2500 620 1456 1201 174 1396
Fragments arranged by size:
2500 1456 1396 1201 1183 620 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 2134 5594 7174 7361 1700
Size: 110 236 88 3460 1580 187 2869
Fragments arranged by size:
3460 2869 1580 236 187 110 88
BsrGI T'GTAC_A
Cuts at: 96 3109 4415 4594 96
Size: 3013 1306 179 4032
Fragments arranged by size:
4032 3013 1306 179
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8530
BssSI C'ACGA_G
Cuts at: 6590 7602 6590
Size: 1012 7518
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3470 794
Size: 2676 5854
BstBI TT'CG_AA
Cuts at: 4330 7334 4330
Size: 3004 5526
BstEII G'GTnAC_C
Cuts at: 1184 3903 1184
Size: 2719 5811
BstXI CCAn_nnnn'nTGG
Cuts at: 2380 2380
Size: 8530
BstYI r'GATC_y
Cuts at: 1972 2470 2948 4462 4483 5860 6227 6612 6629
Size: 498 478 1514 21 1377 367 385 17
Cuts at: 6629 8081 8167 8179 8525 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1514 1452 1377 498 478 385 367
346 86 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 5178 5178
Size: 8530
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2208 513
Size: 552 939 168 36 6835
Fragments arranged by size:
6835 939 552 168 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7791
Fragments arranged by size:
7791 683 56
BtsI GCAGTG_nn'
Cuts at: 3411 5040 6046 6840 6860 3411
Size: 1629 1006 794 20 5081
Fragments arranged by size:
5081 1629 1006 794 20
ClaI AT'CG_AT
Cuts at: 1554 3070 1554
Size: 1516 7014
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8495
DraI TTT'AAA
Cuts at: 3064 4372 4693 5834 3064
Size: 1308 321 1141 5760
Fragments arranged by size:
5760 1308 1141 321
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7537 809
Size: 507 6221 1802
Fragments arranged by size:
6221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 5785 5977
Size: 54 1425 50 275 18 381 3574 192
Cuts at: 5977 6866 8495 8
Size: 889 1629 43
Fragments arranged by size:
3574 1629 1425 889 381 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5977 1537
Size: 275 4165 4090
Fragments arranged by size:
4165 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2393 2574 4776 5526 6460 1353
Size: 1040 181 2202 750 934 3423
Fragments arranged by size:
3423 2202 1040 934 750 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 2212 7117 7491 7637 387
Size: 1064 138 476 147 4905 374 146 1280
Fragments arranged by size:
4905 1280 1064 476 374 147 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2487 2574 2739 5141 5570 6593
Size: 393 381 474 87 165 2402 429 1023
Cuts at: 6593 7190 7977 8245 1239
Size: 597 787 268 1524
Fragments arranged by size:
2402 1524 1023 787 597 474 429 393
381 268 165 87
EcoNI CCTnn'n_nnAGG
Cuts at: 3263 5177 3263
Size: 1914 6616
EcoO109I rG'GnC_Cy
Cuts at: 2014 2015 2014
Size: 1 8529
EcoRI G'AATT_C
Cuts at: 5839 6217 5839
Size: 378 8152
EcoRV GAT'ATC
Cuts at: 2422 2995 2422
Size: 573 7957
FspI TGC'GCA
Cuts at: 7036 7036
Size: 8530
HaeII r_GCGC'y
Cuts at: 1678 2062 5144 7677 1678
Size: 384 3082 2533 2531
Fragments arranged by size:
3082 2533 2531 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 2984 3016 3749
Size: 32 135 32 911 32 469 32 733
Cuts at: 3749 3781 4432 4464 4508 4540 7171 7203 7245
Size: 32 651 32 44 32 2631 32 42
Cuts at: 7245 7277 1373
Size: 32 2626
Fragments arranged by size:
2631 2626 911 733 651 469 135 44
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5269 5508 5874 6120 669
Size: 988 281 3331 239 366 246 3079
Fragments arranged by size:
3331 3079 988 366 281 246 239
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8530
HpaI GTT'AAC
Cuts at: 6120 6120
Size: 8530
KasI G'GCGC_C
Cuts at: 2058 2058
Size: 8530
KpnI G_GTAC'C
Cuts at: 5855 5855
Size: 8530
MfeI C'AATT_G
Cuts at: 6129 6129
Size: 8530
MluI A'CGCG_T
Cuts at: 8406 8406
Size: 8530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 2556 3295 5755 7644 7828 1305
Size: 1053 198 739 2460 1889 184 2007
Fragments arranged by size:
2460 2007 1889 1053 739 198 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 5787 10
Size: 54 1425 343 381 3574 2753
Fragments arranged by size:
3574 2753 1425 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 2814 4129 5007 5063 6488 6847
Size: 648 1212 436 1315 878 56 1425 359
Cuts at: 6847 7006 518
Size: 159 2042
Fragments arranged by size:
2042 1425 1315 1212 878 648 436 359
159 56
MspA1I CmG'CkG
Cuts at: 1606 2174 4421 5946 6623 7771 8016 1606
Size: 568 2247 1525 677 1148 245 2120
Fragments arranged by size:
2247 2120 1525 1148 677 568 245
NaeI GCC'GGC
Cuts at: 2136 5596 2136
Size: 3460 5070
NarI GG'CG_CC
Cuts at: 2059 2059
Size: 8530
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7039
Fragments arranged by size:
7039 939 552
NdeI CA'TA_TG
Cuts at: 387 4973 387
Size: 4586 3944
NgoMIV G'CCGG_C
Cuts at: 2134 5594 2134
Size: 3460 5070
NheI G'CTAG_C
Cuts at: 1052 4475 5404 1052
Size: 3423 929 4178
Fragments arranged by size:
4178 3423 929
NotI GC'GGCC_GC
Cuts at: 5977 5977
Size: 8530
NspI r_CATG'y
Cuts at: 1132 1229 5594 5888 7433 1132
Size: 97 4365 294 1545 2229
Fragments arranged by size:
4365 2229 1545 294 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8530
PciI A'CATG_T
Cuts at: 7429 7429
Size: 8530
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3936 1108
Size: 2828 5702
PmeI GTTT'AAAC
Cuts at: 5834 5834
Size: 8530
PmlI CAC'GTG
Cuts at: 4174 4174
Size: 8530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5556 5588 6599 6631 8112 8144 5556
Size: 32 1011 32 1481 32 5942
Fragments arranged by size:
5942 1481 1011 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8530
PsiI TTA'TAA
Cuts at: 6100 6100
Size: 8530
PspOMI G'GGCC_C
Cuts at: 2014 2014
Size: 8530
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3102 3134 3102
Size: 32 8498
PstI C_TGCA'G
Cuts at: 830 3430 5882 830
Size: 2600 2452 3478
Fragments arranged by size:
3478 2600 2452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8530
PvuII CAG'CTG
Cuts at: 4421 4421
Size: 8530
SacII CC_GC'GG
Cuts at: 2175 2175
Size: 8530
SalI G'TCGA_C
Cuts at: 1936 5872 1936
Size: 3936 4594
SbfI CC_TGCA'GG
Cuts at: 5882 5882
Size: 8530
ScaI AGT'ACT
Cuts at: 2464 2595 2830 4238 6778 8490 2464
Size: 131 235 1408 2540 1712 2504
Fragments arranged by size:
2540 2504 1712 1408 235 131
SfcI C'TryA_G
Cuts at: 826 1045 2853 3136 3148 3426 5305 5878 7013
Size: 219 1808 283 12 278 1879 573 1135
Cuts at: 7013 7694 7885 826
Size: 681 191 1471
Fragments arranged by size:
1879 1808 1471 1135 681 573 283 278
219 191 12
SfoI GGC'GCC
Cuts at: 2060 2060
Size: 8530
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8530
SgrAI Cr'CCGG_yG
Cuts at: 1700 2046 1700
Size: 346 8184
SmaI CCC'GGG
Cuts at: 1872 5857 1872
Size: 3985 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5381 6634 7535 7797 820
Size: 197 926 3438 1253 901 262 1553
Fragments arranged by size:
3438 1553 1253 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6321 493
Size: 5828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8530
SphI G_CATG'C
Cuts at: 1132 5594 5888 1132
Size: 4462 294 3774
Fragments arranged by size:
4462 3774 294
SspI AAT'ATT
Cuts at: 5 52 5816 6454 5
Size: 47 5764 638 2081
Fragments arranged by size:
5764 2081 638 47
StuI AGG'CCT
Cuts at: 1517 3221 1517
Size: 1704 6826
StyI C'CwwG_G
Cuts at: 513 1065 2004 3175 4377 5969 513
Size: 552 939 1171 1202 1592 3074
Fragments arranged by size:
3074 1592 1202 1171 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3922 4703 5491 5637 6557 6574 6727
Size: 1255 2342 781 788 146 920 17 153
Cuts at: 6727 6912 7357 325
Size: 185 445 1498
Fragments arranged by size:
2342 1498 1255 920 788 781 445 185
153 146 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2462 2593 2828
Size: 274 80 33 51 1443 485 131 235
Cuts at: 2828 3109 4236 4415 4594 4950 6776 8488 96
Size: 281 1127 179 179 356 1826 1712 138
Fragments arranged by size:
1826 1712 1443 1127 485 356 281 274
235 179 179 138 131 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5267 5299 8490 8522 5267
Size: 32 3191 32 5275
Fragments arranged by size:
5275 3191 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2639 1316
Size: 1323 7207
XbaI T'CTAG_A
Cuts at: 4615 5801 5866 4615
Size: 1186 65 7279
Fragments arranged by size:
7279 1186 65
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8530
XmaI C'CCGG_G
Cuts at: 1870 5855 1870
Size: 3985 4545
XmnI GAAnn'nnTTC
Cuts at: 4181 4941 6659 4181
Size: 760 1718 6052
Fragments arranged by size:
6052 1718 760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6338 276
Size: 53 83 186 936 4804 2468
Fragments arranged by size:
4804 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI
PfoI PpuMI PspXI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI