Restriction map for
pFN21AA0254
(Circular) (Six-base) MAPSORT of: pFN21AA0254.seq Check: 4818 from: 1 to: 7681
pFN21AA0254 7681 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2508 2508
Size: 7681
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5491 278
Size: 53 83 186 936 3955 2468
Fragments arranged by size:
3955 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2230 5024 1937
Size: 293 2794 4594
Fragments arranged by size:
4594 2794 293
Acc65I G'GTAC_C
Cuts at: 2355 5002 2355
Size: 2647 5034
AclI AA'CG_TT
Cuts at: 1563 5808 6181 1563
Size: 4245 373 3063
Fragments arranged by size:
4245 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 2646 3282 3535 4242 4628 5744 7128
Size: 576 438 636 253 707 386 1116 1384
Cuts at: 7128 7396 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1116 707 636 576 438 386
268 253
AfeI AGC'GCT
Cuts at: 4619 4619
Size: 7681
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7484
AflIII A'CryG_T
Cuts at: 1203 2145 2639 4384 6580 7557 1203
Size: 942 494 1745 2196 977 1327
Fragments arranged by size:
2196 1745 1327 977 942 494
AgeI A'CCGG_T
Cuts at: 6512 6512
Size: 7681
AhdI GACnn_n'nnGTC
Cuts at: 6410 6410
Size: 7681
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3731 3942 1184
Size: 2547 211 4923
Fragments arranged by size:
4923 2547 211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2235 2267 2235
Size: 32 7649
AlwNI CAG_nnn'CTG
Cuts at: 2774 3235 3275 3724 4714 4744 5424 6996 2774
Size: 461 40 449 990 30 680 1572 3459
Fragments arranged by size:
3459 1572 990 680 461 449 40 30
ApaLI G'TGCA_C
Cuts at: 2609 5738 6894 2609
Size: 3129 1156 3396
Fragments arranged by size:
3396 3129 1156
ApoI r'AATT_y
Cuts at: 1493 2653 3328 4197 4776 4886 4990 5216 5368
Size: 1160 675 869 579 110 104 226 152
Cuts at: 5368 7477 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1160 869 675 579 226 152
110 104
AseI AT'TA_AT
Cuts at: 160 1032 6235 160
Size: 872 5203 1606
Fragments arranged by size:
5203 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7681
AvaI C'yCGr_G
Cuts at: 1870 1943 3627 5006 1870
Size: 73 1684 1379 4545
Fragments arranged by size:
4545 1684 1379 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3589 3622 3589
Size: 33 7648
BamHI G'GATC_C
Cuts at: 2781 5011 5378 2781
Size: 2230 367 5084
Fragments arranged by size:
5084 2230 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2355 2674 3614 5002 6457
Size: 325 217 627 568 319 940 1388 1455
Cuts at: 6457 618
Size: 1842
Fragments arranged by size:
1842 1455 1388 940 627 568 325 319
217
BanII G_rGCy'C
Cuts at: 1391 2204 3482 3564 4103 5000 1391
Size: 813 1278 82 539 897 4072
Fragments arranged by size:
4072 1278 897 813 539 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 3117 4076 928
Size: 971 116 1102 959 4533
Fragments arranged by size:
4533 1102 971 959 116
BbvCI CC'TCA_GC
Cuts at: 2889 3276 4178 2889
Size: 387 902 6392
Fragments arranged by size:
6392 902 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5872 5906 1903
Size: 34 3935 34 3678
Fragments arranged by size:
3935 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5573 6783 5573
Size: 1210 6471
BclI T'GATC_A
Cuts at: 1548 2754 1548
Size: 1206 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6292 136
Size: 107 122 71 631 605 4620 1525
Fragments arranged by size:
4620 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3796 3847 4762 7676 3796
Size: 51 915 2914 3801
Fragments arranged by size:
3801 2914 915 51
BlpI GC'TnA_GC
Cuts at: 3541 4802 5098 3541
Size: 1261 296 6124
Fragments arranged by size:
6124 1261 296
Bme1580I G_kGCm'C
Cuts at: 1790 2613 2679 2848 2868 3217 5742 6898 1790
Size: 823 66 169 20 349 2525 1156 2573
Fragments arranged by size:
2573 2525 1156 823 349 169 66 20
BmgBI CAC'GTC
Cuts at: 1318 4385 1318
Size: 3067 4614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2613 2930 3152
Size: 416 53 83 399 24 1196 317 222
Cuts at: 3152 5412 6370 442
Size: 2260 958 1753
Fragments arranged by size:
2260 1753 1196 958 416 399 317 222
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4167 1056
Size: 3111 4570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2937 2937
Size: 7681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2025 2188 2235 2907 2967 3143 3156 3431
Size: 786 163 47 672 60 176 13 275
Cuts at: 3431 3803 4188 4235 4841 6341 1239
Size: 372 385 47 606 1500 2579
Fragments arranged by size:
2579 1500 786 672 606 385 372 275
176 163 60 47 47 13
Bpu10I CC'TnA_GC
Cuts at: 2889 3276 3566 4178 2889
Size: 387 290 612 6392
Fragments arranged by size:
6392 612 387 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5806 6671 6969 5806
Size: 865 298 6518
Fragments arranged by size:
6518 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3322 4027 4121 6344 882
Size: 617 1823 705 94 2223 2219
Fragments arranged by size:
2223 2219 1823 705 617 94
BsaAI yAC'GTr
Cuts at: 493 1204 5472 493
Size: 711 4268 2702
Fragments arranged by size:
4268 2702 711
BsaBI GATnn'nnATC
Cuts at: 7675 7675
Size: 7681
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2707 5488 5870 6505
Size: 53 83 186 936 1174 2781 382 635
Cuts at: 6505 275
Size: 1451
Fragments arranged by size:
2781 1451 1174 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2702 6114 6512 6786 6933 7344 2702
Size: 3412 398 274 147 411 3039
Fragments arranged by size:
3412 3039 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2254 2284 1178
Size: 30 165 30 851 30 6575
Fragments arranged by size:
6575 851 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3293 3508 3800 3968 4124 4478 4928 4966
Size: 2107 215 292 168 156 354 450 38
Cuts at: 4966 1186
Size: 3901
Fragments arranged by size:
3901 2107 450 354 292 215 168 156
38
BseYI C'CCAG_C
Cuts at: 2185 2197 2836 4430 4620 4806 6884 7380 2185
Size: 12 639 1594 190 186 2078 496 2486
Fragments arranged by size:
2486 2078 1594 639 496 190 186 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3980 4610 4646 1163
Size: 573 2244 630 36 4198
Fragments arranged by size:
4198 2244 630 573 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3443 5131 5892 6920 1540
Size: 275 186 1442 1688 761 1028 2301
Fragments arranged by size:
2301 1688 1442 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2613 3482 3636 5742 5827 6898 2613
Size: 869 154 2106 85 1071 3396
Fragments arranged by size:
3396 2106 1071 869 154 85
BsmI GAATG_Cn'
Cuts at: 5192 5285 5192
Size: 93 7588
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2613 2679 2848 2868 3217 3482
Size: 399 414 409 66 169 20 349 265
Cuts at: 3482 3564 3636 4103 5000 5742 5827 6898 1391
Size: 82 72 467 897 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 467 414 409 399
349 265 169 85 82 72 66 20
BspEI T'CCGG_A
Cuts at: 7344 7344
Size: 7681
BspHI T'CATG_A
Cuts at: 3165 5570 7300 3165
Size: 2405 1730 3546
Fragments arranged by size:
3546 2405 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2508 3437 4582 5036 844
Size: 1664 929 1145 454 3489
Fragments arranged by size:
3489 1664 1145 929 454
BsrBI CCG'CTC
Cuts at: 1394 1823 5568 1394
Size: 429 3745 3507
Fragments arranged by size:
3745 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2171 2743 6176 6350 65
Size: 1183 923 572 3433 174 1396
Fragments arranged by size:
3433 1396 1183 923 572 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3313 4797 6325 6512 1700
Size: 110 1503 1484 1528 187 2869
Fragments arranged by size:
2869 1528 1503 1484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7681
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7681
BssSI C'ACGA_G
Cuts at: 2795 5741 6753 2795
Size: 2946 1012 3723
Fragments arranged by size:
3723 2946 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7681
BstBI TT'CG_AA
Cuts at: 6485 6485
Size: 7681
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7681
BstXI CCAn_nnnn'nTGG
Cuts at: 4620 4620
Size: 7681
BstYI r'GATC_y
Cuts at: 1972 2770 2781 2821 3342 3460 3796 3847 4360
Size: 798 11 40 521 118 336 51 513
Cuts at: 4360 4480 4762 4894 5011 5378 5763 5780 7232
Size: 120 282 132 117 367 385 17 1452
Cuts at: 7232 7318 7330 7676 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 798 521 513 385 367 346
336 282 132 120 118 117 86 51
40 17 12 11
Bsu36I CC'TnA_GG
Cuts at: 2280 2280
Size: 7681
BtgI C'CryG_G
Cuts at: 513 1065 4685 513
Size: 552 3620 3509
Fragments arranged by size:
3620 3509 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4307 487
Size: 683 56 3081 3861
Fragments arranged by size:
3861 3081 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 4267 5197 5991 6011 2529
Size: 1738 930 794 20 4199
Fragments arranged by size:
4199 1738 930 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7681
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7646
DraI TTT'AAA
Cuts at: 4985 4985
Size: 7681
DraIII CAC_nnn'GTG
Cuts at: 3739 4214 3739
Size: 475 7206
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6688 809
Size: 507 5372 1802
Fragments arranged by size:
5372 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 3440 5128
Size: 54 1425 50 275 18 429 1181 1688
Cuts at: 5128 6017 7646 8
Size: 889 1629 43
Fragments arranged by size:
1688 1629 1425 1181 889 429 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3440 5128 1537
Size: 275 1628 1688 4090
Fragments arranged by size:
4090 1688 1628 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5611 1353
Size: 4258 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6268 6642 6788 387
Size: 1064 138 4679 374 146 1280
Fragments arranged by size:
4679 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3480 3480
Size: 7681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2025 2188 2208 2235 2646 2907 2967
Size: 393 393 163 20 27 411 261 60
Cuts at: 2967 3143 3156 3282 3431 3535 3803 4188 4235
Size: 176 13 126 149 104 268 385 47
Cuts at: 4235 4242 4628 4841 5744 6341 7128 7396 1239
Size: 7 386 213 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 597 411 393 393 386
385 268 268 261 213 176 163 149
126 104 60 47 27 20 13 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 4377 2570
Size: 1807 5874
EcoO109I rG'GnC_Cy
Cuts at: 2975 3092 3827 4563 4698 2975
Size: 117 735 736 135 5958
Fragments arranged by size:
5958 736 735 135 117
EcoRI G'AATT_C
Cuts at: 3328 4886 4990 5368 3328
Size: 1558 104 378 5641
Fragments arranged by size:
5641 1558 378 104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 7681
FspI TGC'GCA
Cuts at: 6187 6187
Size: 7681
HaeII r_GCGC'y
Cuts at: 1678 4621 6828 1678
Size: 2943 2207 2531
Fragments arranged by size:
2943 2531 2207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2440 2472 3110 3142 3464
Size: 32 135 32 868 32 638 32 322
Cuts at: 3464 3496 4265 4297 4795 4827 6322 6354 6396
Size: 32 769 32 498 32 1495 32 42
Cuts at: 6396 6428 1373
Size: 32 2626
Fragments arranged by size:
2626 1495 868 769 638 498 322 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 2231 5025 5271 669
Size: 988 281 205 88 2794 246 3079
Fragments arranged by size:
3079 2794 988 281 246 205 88
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7681
HpaI GTT'AAC
Cuts at: 5271 5271
Size: 7681
KpnI G_GTAC'C
Cuts at: 2359 5006 2359
Size: 2647 5034
MfeI C'AATT_G
Cuts at: 5280 5280
Size: 7681
MluI A'CGCG_T
Cuts at: 2639 7557 2639
Size: 4918 2763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6795 6979 1305
Size: 5490 184 2007
Fragments arranged by size:
5490 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 10
Size: 54 1425 343 429 5430
Fragments arranged by size:
5430 1425 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 3164 5639 5998 6157 518
Size: 648 1158 840 2475 359 159 2042
Fragments arranged by size:
2475 2042 1158 840 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2296 3133 3711 3724 5097 5774 6922
Size: 439 251 837 578 13 1373 677 1148
Cuts at: 6922 7167 1606
Size: 245 2120
Fragments arranged by size:
2120 1373 1148 837 677 578 439 251
245 13
NaeI GCC'GGC
Cuts at: 4799 4799
Size: 7681
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7129
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7681
NgoMIV G'CCGG_C
Cuts at: 4797 4797
Size: 7681
NheI G'CTAG_C
Cuts at: 1052 4163 1052
Size: 3111 4570
NotI GC'GGCC_GC
Cuts at: 5128 5128
Size: 7681
NspI r_CATG'y
Cuts at: 1132 1229 2736 4537 5039 6584 1132
Size: 97 1507 1801 502 1545 2229
Fragments arranged by size:
2229 1801 1545 1507 502 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7681
PciI A'CATG_T
Cuts at: 6580 6580
Size: 7681
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2716 3235 1108
Size: 1608 519 5554
Fragments arranged by size:
5554 1608 519
PfoI T'CCnGG_A
Cuts at: 2031 2421 4251 4444 4818 4881 2031
Size: 390 1830 193 374 63 4831
Fragments arranged by size:
4831 1830 390 374 193 63
PmeI GTTT'AAAC
Cuts at: 4985 4985
Size: 7681
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5750 5782 7263 7295 5750
Size: 32 1481 32 6136
Fragments arranged by size:
6136 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3092 3827 4563 4698 3092
Size: 735 736 135 6075
Fragments arranged by size:
6075 736 735 135
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7681
PsiI TTA'TAA
Cuts at: 5251 5251
Size: 7681
PstI C_TGCA'G
Cuts at: 830 2505 2536 2974 3720 3774 5033 830
Size: 1675 31 438 746 54 1259 3478
Fragments arranged by size:
3478 1675 1259 746 438 54 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7681
PvuII CAG'CTG
Cuts at: 2045 2296 3724 2045
Size: 251 1428 6002
Fragments arranged by size:
6002 1428 251
SacI G_AGCT'C
Cuts at: 3482 3482
Size: 7681
SalI G'TCGA_C
Cuts at: 1936 2229 5023 1936
Size: 293 2794 4594
Fragments arranged by size:
4594 2794 293
SanDI GG'GwC_CC
Cuts at: 4563 4563
Size: 7681
SbfI CC_TGCA'GG
Cuts at: 5033 5033
Size: 7681
ScaI AGT'ACT
Cuts at: 5929 7641 5929
Size: 1712 5969
SexAI A'CCwGG_T
Cuts at: 3526 3526
Size: 7681
SfcI C'TryA_G
Cuts at: 826 1045 2501 2532 2970 3009 3074 3716 3770
Size: 219 1456 31 438 39 65 642 54
Cuts at: 3770 5029 6164 6845 7036 826
Size: 1259 1135 681 191 1471
Fragments arranged by size:
1471 1456 1259 1135 681 642 438 219
191 65 54 39 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7681
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7681
SmaI CCC'GGG
Cuts at: 1872 5008 1872
Size: 3136 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5785 6686 6948 820
Size: 197 926 3842 901 262 1553
Fragments arranged by size:
3842 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5472 493
Size: 4979 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7681
SphI G_CATG'C
Cuts at: 1132 5039 1132
Size: 3907 3774
SspI AAT'ATT
Cuts at: 5 52 5605 5
Size: 47 5553 2081
Fragments arranged by size:
5553 2081 47
StuI AGG'CCT
Cuts at: 1517 3309 1517
Size: 1792 5889
StyI C'CwwG_G
Cuts at: 513 1065 2811 4867 5120 513
Size: 552 1746 2056 253 3074
Fragments arranged by size:
3074 2056 1746 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3014 3669 4145 4612 4958 5708 5725
Size: 1255 1434 655 476 467 346 750 17
Cuts at: 5725 5878 6063 6508 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1434 1255 750 655 476 467 445
346 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2791 5927 7639
Size: 274 80 33 51 1443 814 3136 1712
Cuts at: 7639 96
Size: 138
Fragments arranged by size:
3136 1712 1443 814 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2254 2286 3194 3226 7641 7673 2254
Size: 32 908 32 4415 32 2262
Fragments arranged by size:
4415 2262 908 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7681
XbaI T'CTAG_A
Cuts at: 4025 5017 4025
Size: 992 6689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2413 2686 4291 4384 2413
Size: 273 1605 93 5710
Fragments arranged by size:
5710 1605 273 93
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7681
XmaI C'CCGG_G
Cuts at: 1870 5006 1870
Size: 3136 4545
XmnI GAAnn'nnTTC
Cuts at: 5810 5810
Size: 7681
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5489 276
Size: 53 83 186 936 3955 2468
Fragments arranged by size:
3955 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI
SfoI SrfI SwaI