Restriction map for
pFN21AA0247
(Circular) (Six-base) MAPSORT of: pFN21AA0247.seq Check: 1764 from: 1 to: 5614
pFN21AA0247 5614 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2584 2672 2584
Size: 88 5526
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2462 3677 5061 5329 1632
Size: 618 212 1215 1384 268 1917
Fragments arranged by size:
1917 1384 1215 618 268 212
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 2238 2267 4513 5490 1203
Size: 1035 29 2246 977 1327
Fragments arranged by size:
2246 1327 1035 977 29
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 2039 1166
Size: 873 4741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AlwNI CAG_nnn'CTG
Cuts at: 2750 3357 4929 2750
Size: 607 1572 3435
Fragments arranged by size:
3435 1572 607
ApaI G_GGCC'C
Cuts at: 2622 2622
Size: 5614
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2923 3149 3301 5410 1493
Size: 1430 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2736 2939 1870
Size: 73 793 203 4545
Fragments arranged by size:
4545 793 203 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 4390 618
Size: 325 217 627 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2622 2735 2933 1391
Size: 1231 113 198 4072
Fragments arranged by size:
4072 1231 198 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2194 2228 3805 3839 1903
Size: 34 257 34 1577 34 3678
Fragments arranged by size:
3678 1577 257 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4225 136
Size: 107 122 71 631 605 2553 1525
Fragments arranged by size:
2553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2622 3675 4831 1790
Size: 342 490 1053 1156 2573
Fragments arranged by size:
2573 1156 1053 490 342
BmgBI CAC'GTC
Cuts at: 1318 2268 2871 1318
Size: 950 603 4061
Fragments arranged by size:
4061 950 603
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2616 3345 4303
Size: 416 53 83 399 24 1199 729 958
Cuts at: 4303 442
Size: 1753
Fragments arranged by size:
1753 1199 958 729 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4274 1239
Size: 3035 2579
Bpu10I CC'TnA_GC
Cuts at: 2794 2794
Size: 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3739 4604 4902 3739
Size: 865 298 4451
Fragments arranged by size:
4451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2181 2497 4277 882
Size: 617 682 316 1780 2219
Fragments arranged by size:
2219 1780 682 617 316
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2140 4047 4445 4719 4866 5277 2140
Size: 1907 398 274 147 411 2477
Fragments arranged by size:
2477 1907 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2477 2502 2507 2532 1178
Size: 30 165 30 1074 25 5 25 4260
Fragments arranged by size:
4260 1074 165 30 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2396 2552 2644 2665 2891 1186
Size: 1207 3 156 92 21 226 3909
Fragments arranged by size:
3909 1207 226 156 92 21 3
BseYI C'CCAG_C
Cuts at: 4817 5313 4817
Size: 496 5118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5041
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 3675 3760 4831 2036
Size: 1639 85 1071 2819
Fragments arranged by size:
2819 1639 1071 85
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
BsmBI CGTCTCn'nnnn_
Cuts at: 2103 2318 2103
Size: 215 5399
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2132 2622 2735 2933 3675 3760
Size: 399 246 96 490 113 198 742 85
Cuts at: 3760 4831 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 246 198 113
96 85
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2584 2672 2969 844
Size: 1740 88 297 3489
Fragments arranged by size:
3489 1740 297 88
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4109 4283 65
Size: 1183 2861 174 1396
Fragments arranged by size:
2861 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2789 4258 4445 1700
Size: 110 979 1469 187 2869
Fragments arranged by size:
2869 1469 979 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 2408 3674 4686 2408
Size: 1266 1012 3336
Fragments arranged by size:
3336 1266 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2653 794
Size: 1859 3755
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstXI CCAn_nnnn'nTGG
Cuts at: 2389 2503 2389
Size: 114 5500
BstYI r'GATC_y
Cuts at: 1972 2944 3311 3696 3713 5165 5251 5263 5609
Size: 972 367 385 17 1452 86 12 346
Cuts at: 5609 1972
Size: 1977
Fragments arranged by size:
1977 1452 972 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2011 2049 2058 2295 2388 513
Size: 552 946 38 9 237 93 3739
Fragments arranged by size:
3739 946 552 237 93 38 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 2212 3130 3924 3944 2212
Size: 918 794 20 3882
Fragments arranged by size:
3882 918 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DraIII CAC_nnn'GTG
Cuts at: 2569 2569
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2787 3061 3950
Size: 54 1425 50 275 18 957 274 889
Cuts at: 3950 5579 8
Size: 1629 43
Fragments arranged by size:
1629 1425 957 889 275 274 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2782 3544 1353
Size: 1429 762 3423
Fragments arranged by size:
3423 1429 762
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2462 3677 4274 5061 5329 1239
Size: 393 618 212 1215 597 787 268 1524
Fragments arranged by size:
1524 1215 787 618 597 393 268 212
EcoO109I rG'GnC_Cy
Cuts at: 2350 2667 2698 2350
Size: 317 31 5266
Fragments arranged by size:
5266 317 31
EcoRI G'AATT_C
Cuts at: 2923 3301 2923
Size: 378 5236
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 2396 4761 1678
Size: 718 2365 2531
Fragments arranged by size:
2531 2365 718
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 2746 2778 4255
Size: 32 135 32 905 32 237 32 1477
Cuts at: 4255 4287 4329 4361 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1477 905 237 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3792
Fragments arranged by size:
3792 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2501 3572 3931 4090 518
Size: 648 873 462 1071 359 159 2042
Fragments arranged by size:
2042 1071 873 648 462 359 159
MspA1I CmG'CkG
Cuts at: 1606 2653 3030 3707 4855 5100 1606
Size: 1047 377 677 1148 245 2120
Fragments arranged by size:
2120 1148 1047 677 377 245
NaeI GCC'GGC
Cuts at: 2791 2791
Size: 5614
NcoI C'CATG_G
Cuts at: 513 1065 2011 2295 513
Size: 552 946 284 3832
Fragments arranged by size:
3832 946 552 284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NgoMIV G'CCGG_C
Cuts at: 2789 2789
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2242 2972 4517 1132
Size: 97 1013 730 1545 2229
Fragments arranged by size:
2229 1545 1013 730 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 2238 4513 2238
Size: 2275 3339
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2153 1108
Size: 1045 4569
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2350 2350
Size: 5614
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PspOMI G'GGCC_C
Cuts at: 2618 2618
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2186 2433 2683 2830 2966 830
Size: 1356 247 250 147 136 3478
Fragments arranged by size:
3478 1356 250 247 147 136
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
PvuII CAG'CTG
Cuts at: 2653 2653
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SanDI GG'GwC_CC
Cuts at: 2350 2350
Size: 5614
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SexAI A'CCwGG_T
Cuts at: 2487 2487
Size: 5614
SfcI C'TryA_G
Cuts at: 826 1045 2182 2364 2429 2679 2826 2962 4097
Size: 219 1137 182 65 250 147 136 1135
Cuts at: 4097 4778 4969 826
Size: 681 191 1471
Fragments arranged by size:
1471 1137 1135 681 250 219 191 182
147 136 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3718 4619 4881 820
Size: 197 926 1775 901 262 1553
Fragments arranged by size:
1775 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2011 2295 2639 2662 3053 513
Size: 552 946 284 344 23 391 3074
Fragments arranged by size:
3074 946 552 391 344 284 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2348 2773 3641 3658 3811 3996 4441
Size: 1255 768 425 868 17 153 185 445
Cuts at: 4441 325
Size: 1498
Fragments arranged by size:
1498 1255 868 768 445 425 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2590 2880 3860
Size: 274 80 33 51 1443 613 290 980
Cuts at: 3860 5572 96
Size: 1712 138
Fragments arranged by size:
1712 1443 980 613 290 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 2334 2366 5574 5606 2055
Size: 32 247 32 3208 32 2063
Fragments arranged by size:
3208 2063 247 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2489 1316
Size: 1173 4441
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2342 2477 2342
Size: 135 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 2447 3743 2447
Size: 1296 4318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI