Restriction map for
pFN21AA0240
(Circular) (Six-base) MAPSORT of: pFN21AA0240.seq Check: 634 from: 1 to: 7942
pFN21AA0240 7942 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4039 4039
Size: 7942
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5752 278
Size: 53 83 186 936 4216 2468
Fragments arranged by size:
4216 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 4449 5285 1937
Size: 1848 664 836 4594
Fragments arranged by size:
4594 1848 836 664
Acc65I G'GTAC_C
Cuts at: 5263 5263
Size: 7942
AclI AA'CG_TT
Cuts at: 1563 6069 6442 1563
Size: 4506 373 3063
Fragments arranged by size:
4506 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 3781 4260 4779 4808 6005 7389 7657
Size: 674 1475 479 519 29 1197 1384 268
Cuts at: 7657 1632
Size: 1917
Fragments arranged by size:
1917 1475 1384 1197 674 519 479 268
29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7745
AflIII A'CryG_T
Cuts at: 1203 2575 3078 4215 4904 6841 7818 1203
Size: 1372 503 1137 689 1937 977 1327
Fragments arranged by size:
1937 1372 1327 1137 977 689 503
AgeI A'CCGG_T
Cuts at: 4604 6773 4604
Size: 2169 5773
AhdI GACnn_n'nnGTC
Cuts at: 6671 6671
Size: 7942
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3453 3959 4577 1184
Size: 2269 506 618 4549
Fragments arranged by size:
4549 2269 618 506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2797 2798 2829 2830 2797
Size: 1 31 1 7909
Fragments arranged by size:
7909 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2491 4201 5685 7257 2491
Size: 1710 1484 1572 3176
Fragments arranged by size:
3176 1710 1572 1484
ApaI G_GGCC'C
Cuts at: 2220 2220
Size: 7942
ApaLI G'TGCA_C
Cuts at: 5999 7155 5999
Size: 1156 6786
ApoI r'AATT_y
Cuts at: 1493 2140 2860 2917 3000 4268 4718 4939 5251
Size: 647 720 57 83 1268 450 221 312
Cuts at: 5251 5477 5629 7738 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1268 720 647 450 312 226
221 152 83 57
AseI AT'TA_AT
Cuts at: 160 1032 2688 2937 6496 160
Size: 872 1656 249 3559 1606
Fragments arranged by size:
3559 1656 1606 872 249
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7942
AvaI C'yCGr_G
Cuts at: 1870 1943 2270 4425 5267 1870
Size: 73 327 2155 842 4545
Fragments arranged by size:
4545 2155 842 327 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3723 3756 3723
Size: 33 7909
BamHI G'GATC_C
Cuts at: 5272 5639 5272
Size: 367 7575
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4821 5263 6718 618
Size: 325 217 627 3034 442 1455 1842
Fragments arranged by size:
3034 1842 1455 627 442 325 217
BanII G_rGCy'C
Cuts at: 1391 2220 2763 3062 3930 4229 5261 1391
Size: 829 543 299 868 299 1032 4072
Fragments arranged by size:
4072 1032 868 829 543 299 299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2268 3574 3755 4251 928
Size: 971 369 1306 181 496 4619
Fragments arranged by size:
4619 1306 971 496 369 181
BbvCI CC'TCA_GC
Cuts at: 3480 3480
Size: 7942
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6133 6167 1903
Size: 34 4196 34 3678
Fragments arranged by size:
4196 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5240 5834 7044 5240
Size: 594 1210 6138
Fragments arranged by size:
6138 1210 594
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7942
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6553 136
Size: 107 122 71 631 605 4881 1525
Fragments arranged by size:
4881 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5002 7937 5002
Size: 2935 5007
BlpI GC'TnA_GC
Cuts at: 2421 3637 5359 2421
Size: 1216 1722 5004
Fragments arranged by size:
5004 1722 1216
Bme1580I G_kGCm'C
Cuts at: 1790 2220 4824 6003 7159 1790
Size: 430 2604 1179 1156 2573
Fragments arranged by size:
2604 2573 1179 1156 430
BmgBI CAC'GTC
Cuts at: 1318 3253 4755 4905 1318
Size: 1935 1502 150 4355
Fragments arranged by size:
4355 1935 1502 150
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2214 3156 5673
Size: 416 53 83 399 24 797 942 2517
Cuts at: 5673 6631 442
Size: 958 1753
Fragments arranged by size:
2517 1753 958 942 797 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7942
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4800 4959 4800
Size: 159 7783
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 3720 4103 4991 6602 1239
Size: 995 1486 383 888 1611 2579
Fragments arranged by size:
2579 1611 1486 995 888 383
Bpu10I CC'TnA_GC
Cuts at: 3359 3480 3359
Size: 121 7821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4270 6067 6932 7230 3952
Size: 318 1797 865 298 4664
Fragments arranged by size:
4664 1797 865 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2040 4584 4669 6605 882
Size: 617 541 2544 85 1936 2219
Fragments arranged by size:
2544 2219 1936 617 541 85
BsaAI yAC'GTr
Cuts at: 493 1204 3016 4216 5733 493
Size: 711 1812 1200 1517 2702
Fragments arranged by size:
2702 1812 1517 1200 711
BsaBI GATnn'nnATC
Cuts at: 7936 7936
Size: 7942
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5749 6131 6766 275
Size: 53 83 186 936 4216 382 635 1451
Fragments arranged by size:
4216 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4604 4824 6375 6773 7047 7194 7605 4604
Size: 220 1551 398 274 147 411 4941
Fragments arranged by size:
4941 1551 411 398 274 220 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4889 4919 1178
Size: 30 165 30 3486 30 4201
Fragments arranged by size:
4201 3486 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2271 3468 1186
Size: 1085 1197 5660
Fragments arranged by size:
5660 1197 1085
BseYI C'CCAG_C
Cuts at: 2594 3720 3822 4995 7145 7641 2594
Size: 1126 102 1173 2150 496 2895
Fragments arranged by size:
2895 2150 1173 1126 496 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4067 4760 1163
Size: 573 2331 693 4345
Fragments arranged by size:
4345 2331 693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5392 6153 7181 1540
Size: 275 186 3391 761 1028 2301
Fragments arranged by size:
3391 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3938 4714 4754 4805 6003 6088 7159 3938
Size: 776 40 51 1198 85 1071 4721
Fragments arranged by size:
4721 1198 1071 776 85 51 40
BsmI GAATG_Cn'
Cuts at: 4703 5453 5546 4703
Size: 750 93 7099
Fragments arranged by size:
7099 750 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2763 3062 3930 3938 4229 4714
Size: 399 430 543 299 868 8 291 485
Cuts at: 4714 4754 4805 4824 5261 6003 6088 7159 1391
Size: 40 51 19 437 742 85 1071 2174
Fragments arranged by size:
2174 1071 868 742 543 485 437 430
399 299 291 85 51 40 19 8
BspEI T'CCGG_A
Cuts at: 7605 7605
Size: 7942
BspHI T'CATG_A
Cuts at: 4507 4627 4974 5831 7561 4507
Size: 120 347 857 1730 4888
Fragments arranged by size:
4888 1730 857 347 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4039 5297 844
Size: 3195 1258 3489
Fragments arranged by size:
3489 3195 1258
BsrBI CCG'CTC
Cuts at: 1394 1823 5829 1394
Size: 429 4006 3507
Fragments arranged by size:
4006 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2104 6437 6611 65
Size: 1183 856 4333 174 1396
Fragments arranged by size:
4333 1396 1183 856 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4604 6586 6773 1700
Size: 110 2794 1982 187 2869
Fragments arranged by size:
2869 2794 1982 187 110
BsrGI T'GTAC_A
Cuts at: 96 2554 96
Size: 2458 5484
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7942
BssSI C'ACGA_G
Cuts at: 2023 6002 7014 2023
Size: 3979 1012 2951
Fragments arranged by size:
3979 2951 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2534 794
Size: 1740 6202
BstBI TT'CG_AA
Cuts at: 6746 6746
Size: 7942
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7942
BstYI r'GATC_y
Cuts at: 1972 2702 3027 3918 5002 5272 5639 6024 6041
Size: 730 325 891 1084 270 367 385 17
Cuts at: 6041 7493 7579 7591 7937 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1084 891 730 385 367 346
325 270 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3905 3985 4455 4815 3905
Size: 80 470 360 7032
Fragments arranged by size:
7032 470 360 80
BtgI C'CryG_G
Cuts at: 513 1065 2004 3174 5088 513
Size: 552 939 1170 1914 3367
Fragments arranged by size:
3367 1914 1170 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7203
Fragments arranged by size:
7203 683 56
BtsI GCAGTG_nn'
Cuts at: 3141 3645 3941 5458 6252 6272 3141
Size: 504 296 1517 794 20 4811
Fragments arranged by size:
4811 1517 794 504 296 20
ClaI AT'CG_AT
Cuts at: 1554 4386 1554
Size: 2832 5110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2040 2075 529
Size: 35 1476 35 6396
Fragments arranged by size:
6396 1476 35 35
DraI TTT'AAA
Cuts at: 5246 5246
Size: 7942
DraIII CAC_nnn'GTG
Cuts at: 3421 4150 4184 4660 3421
Size: 729 34 476 6703
Fragments arranged by size:
6703 729 476 34
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3604 6949 809
Size: 507 2288 3345 1802
Fragments arranged by size:
3345 2288 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3307 4033 4522
Size: 54 1425 50 275 18 1477 726 489
Cuts at: 4522 5389 6278 7907 8
Size: 867 889 1629 43
Fragments arranged by size:
1629 1477 1425 889 867 726 489 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5389 1537
Size: 275 3577 4090
Fragments arranged by size:
4090 3577 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 2364 3352 4755 5872 1353
Size: 936 75 988 1403 1117 3423
Fragments arranged by size:
3423 1403 1117 988 936 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6529 6903 7049 387
Size: 1064 138 4940 374 146 1280
Fragments arranged by size:
4940 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2306 3720 3781 4103 4260 4779
Size: 393 602 72 1414 61 322 157 519
Cuts at: 4779 4808 4991 6005 6602 7389 7657 1239
Size: 29 183 1014 597 787 268 1524
Fragments arranged by size:
1524 1414 1014 787 602 597 519 393
322 268 183 157 72 61 29
EcoNI CCTnn'n_nnAGG
Cuts at: 3813 3813
Size: 7942
EcoO109I rG'GnC_Cy
Cuts at: 2216 3611 4302 2216
Size: 1395 691 5856
Fragments arranged by size:
5856 1395 691
EcoRI G'AATT_C
Cuts at: 3000 4939 5251 5629 3000
Size: 1939 312 378 5313
Fragments arranged by size:
5313 1939 378 312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2441 4331 5062 2441
Size: 1890 731 5321
Fragments arranged by size:
5321 1890 731
FspI TGC'GCA
Cuts at: 6448 6448
Size: 7942
HaeII r_GCGC'y
Cuts at: 1678 7089 1678
Size: 5411 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2162 2194 2694
Size: 32 135 32 434 32 124 32 500
Cuts at: 2694 2726 3451 3483 4320 4352 6583 6615 6657
Size: 32 725 32 837 32 2231 32 42
Cuts at: 6657 6689 1373
Size: 32 2626
Fragments arranged by size:
2626 2231 837 725 500 434 135 124
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5286 5532 669
Size: 988 281 3348 246 3079
Fragments arranged by size:
3348 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7942
HpaI GTT'AAC
Cuts at: 5532 5532
Size: 7942
KpnI G_GTAC'C
Cuts at: 5267 5267
Size: 7942
MfeI C'AATT_G
Cuts at: 5541 5541
Size: 7942
MluI A'CGCG_T
Cuts at: 7818 7818
Size: 7942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2503 3261 3375 4000 7056 7240 1305
Size: 1198 758 114 625 3056 184 2007
Fragments arranged by size:
3056 2007 1198 758 625 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3309 4035 10
Size: 54 1425 343 1477 726 3917
Fragments arranged by size:
3917 1477 1425 726 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3083 5900 6259 6418 518
Size: 648 1917 2817 359 159 2042
Fragments arranged by size:
2817 2042 1917 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2727 3715 4043 5011 5219 5358 6035 7183
Size: 1121 988 328 968 208 139 677 1148
Cuts at: 7183 7428 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1121 988 968 677 328 245
208 139
NcoI C'CATG_G
Cuts at: 513 1065 2004 3174 513
Size: 552 939 1170 5281
Fragments arranged by size:
5281 1170 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7942
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7942
NotI GC'GGCC_GC
Cuts at: 5389 5389
Size: 7942
NspI r_CATG'y
Cuts at: 1132 1229 2516 2579 3082 3329 3358 3607 5300
Size: 97 1287 63 503 247 29 249 1693
Cuts at: 5300 6845 1132
Size: 1545 2229
Fragments arranged by size:
2229 1693 1545 1287 503 249 247 97
63 29
PasI CC'CwG_GG
Cuts at: 1348 3055 3923 1348
Size: 1707 868 5367
Fragments arranged by size:
5367 1707 868
PciI A'CATG_T
Cuts at: 2575 3078 6841 2575
Size: 503 3763 3676
Fragments arranged by size:
3763 3676 503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3380 1108
Size: 2272 5670
PfoI T'CCnGG_A
Cuts at: 4920 5169 4920
Size: 249 7693
PmeI GTTT'AAAC
Cuts at: 5246 5246
Size: 7942
PmlI CAC'GTG
Cuts at: 4216 4216
Size: 7942
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 6011 6043 7524 7556 3329
Size: 32 2650 32 1481 32 3715
Fragments arranged by size:
3715 2650 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3611 4302 3611
Size: 691 7251
PshAI GACnn'nnGTC
Cuts at: 1613 2025 4164 4929 1613
Size: 412 2139 765 4626
Fragments arranged by size:
4626 2139 765 412
PsiI TTA'TAA
Cuts at: 5512 5512
Size: 7942
PspOMI G'GGCC_C
Cuts at: 2216 2216
Size: 7942
PspXI vC'TCGA_Gb
Cuts at: 2270 2270
Size: 7942
PstI C_TGCA'G
Cuts at: 830 2131 2496 3673 4672 5294 830
Size: 1301 365 1177 999 622 3478
Fragments arranged by size:
3478 1301 1177 999 622 365
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7942
PvuII CAG'CTG
Cuts at: 3715 4043 5011 5219 3715
Size: 328 968 208 6438
Fragments arranged by size:
6438 968 328 208
SalI G'TCGA_C
Cuts at: 1936 5284 1936
Size: 3348 4594
SbfI CC_TGCA'GG
Cuts at: 5294 5294
Size: 7942
ScaI AGT'ACT
Cuts at: 6190 7902 6190
Size: 1712 6230
SfcI C'TryA_G
Cuts at: 826 1045 2127 2492 2499 3669 4668 5290 6425
Size: 219 1082 365 7 1170 999 622 1135
Cuts at: 6425 7106 7297 826
Size: 681 191 1471
Fragments arranged by size:
1471 1170 1135 1082 999 681 622 365
219 191 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7942
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7942
SmaI CCC'GGG
Cuts at: 1872 5269 1872
Size: 3397 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 3931 4249 6046 6947 7209
Size: 197 926 327 1661 318 1797 901 262
Cuts at: 7209 820
Size: 1553
Fragments arranged by size:
1797 1661 1553 926 901 327 318 262
197
SnaBI TAC'GTA
Cuts at: 493 5733 493
Size: 5240 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7942
SphI G_CATG'C
Cuts at: 1132 5300 1132
Size: 4168 3774
SspI AAT'ATT
Cuts at: 5 52 5866 5
Size: 47 5814 2081
Fragments arranged by size:
5814 2081 47
StuI AGG'CCT
Cuts at: 1517 4081 1517
Size: 2564 5378
StyI C'CwwG_G
Cuts at: 513 1065 2004 3174 3562 4575 4582 4702 5381
Size: 552 939 1170 388 1013 7 120 679
Cuts at: 5381 513
Size: 3074
Fragments arranged by size:
3074 1170 1013 939 679 552 388 120
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2540 3400 3479 4608 5969 5986 6139
Size: 1255 960 860 79 1129 1361 17 153
Cuts at: 6139 6324 6769 325
Size: 185 445 1498
Fragments arranged by size:
1498 1361 1255 1129 960 860 445 185
153 79 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2554 6188 7900
Size: 274 80 33 51 1443 577 3634 1712
Cuts at: 7900 96
Size: 138
Fragments arranged by size:
3634 1712 1443 577 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3441 3473 3637 3669 4889 4921 7902 7934 3441
Size: 32 164 32 1220 32 2981 32 3449
Fragments arranged by size:
3449 2981 1220 164 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3615 1316
Size: 2299 5643
XbaI T'CTAG_A
Cuts at: 5278 5278
Size: 7942
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3263 3377 3422 3569 3659 3263
Size: 114 45 147 90 7546
Fragments arranged by size:
7546 147 114 90 45
XhoI C'TCGA_G
Cuts at: 1943 2270 1943
Size: 327 7615
XmaI C'CCGG_G
Cuts at: 1870 5267 1870
Size: 3397 4545
XmnI GAAnn'nnTTC
Cuts at: 3346 4681 6071 3346
Size: 1335 1390 5217
Fragments arranged by size:
5217 1390 1335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5750 276
Size: 53 83 186 936 4216 2468
Fragments arranged by size:
4216 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI