Restriction map for
pFN21AA0234
(Circular) (Six-base) MAPSORT of: pFN21AA0234.seq Check: 1036 from: 1 to: 9151
pFN21AA0234 9151 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4555 4848 5359 4555
Size: 293 511 8347
Fragments arranged by size:
8347 511 293
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 6961 278
Size: 53 83 186 936 2305 3120 2468
Fragments arranged by size:
3120 2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2464 3841 5772 6494 1937
Size: 527 1377 1931 722 4594
Fragments arranged by size:
4594 1931 1377 722 527
Acc65I G'GTAC_C
Cuts at: 6472 6472
Size: 9151
AclI AA'CG_TT
Cuts at: 1563 6192 7278 7651 1563
Size: 4629 1086 373 3063
Fragments arranged by size:
4629 3063 1086 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2964 3660 4112 4223 4268 4541 4563 5612
Size: 1332 696 452 111 45 273 22 1049
Cuts at: 5612 5709 5763 6203 6372 7214 8598 8866 1632
Size: 97 54 440 169 842 1384 268 1917
Fragments arranged by size:
1917 1384 1332 1049 842 696 452 440
273 268 169 111 97 54 45 22
AfeI AGC'GCT
Cuts at: 4739 4739
Size: 9151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8954
AflIII A'CryG_T
Cuts at: 1203 2673 8050 9027 1203
Size: 1470 5377 977 1327
Fragments arranged by size:
5377 1470 1327 977
AgeI A'CCGG_T
Cuts at: 7982 7982
Size: 9151
AhdI GACnn_n'nnGTC
Cuts at: 7880 7880
Size: 9151
AleI CACnn'nnGTG
Cuts at: 1166 2041 2754 1166
Size: 875 713 7563
Fragments arranged by size:
7563 875 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3314 5682 1184
Size: 2130 2368 4653
Fragments arranged by size:
4653 2368 2130
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4341 4373 4341
Size: 32 9119
AlwNI CAG_nnn'CTG
Cuts at: 3797 4246 4525 4606 5117 5228 5596 5786 6042
Size: 449 279 81 511 111 368 190 256
Cuts at: 6042 6052 6894 8466 3797
Size: 10 842 1572 4482
Fragments arranged by size:
4482 1572 842 511 449 368 279 256
190 111 81 10
ApaLI G'TGCA_C
Cuts at: 3800 7208 8364 3800
Size: 3408 1156 4587
Fragments arranged by size:
4587 3408 1156
ApoI r'AATT_y
Cuts at: 1493 2072 2276 2286 3074 3146 5071 5516 6084
Size: 579 204 10 788 72 1925 445 568
Cuts at: 6084 6110 6460 6686 6838 8947 1493
Size: 26 350 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 788 579 568 445 350
226 204 152 72 26 10
AseI AT'TA_AT
Cuts at: 160 1032 7705 160
Size: 872 6673 1606
Fragments arranged by size:
6673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9151
AvaI C'yCGr_G
Cuts at: 1870 1943 4701 6476 1870
Size: 73 2758 1775 4545
Fragments arranged by size:
4545 2758 1775 73
BamHI G'GATC_C
Cuts at: 6481 6848 6481
Size: 367 8784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2904 3049 3298 4537 4664
Size: 325 217 627 1117 145 249 1239 127
Cuts at: 4664 5320 5371 6472 7927 618
Size: 656 51 1101 1455 1842
Fragments arranged by size:
1842 1455 1239 1117 1101 656 627 325
249 217 145 127 51
BanII G_rGCy'C
Cuts at: 1391 2715 3744 4071 4631 5900 5977 5991 6148
Size: 1324 1029 327 560 1269 77 14 157
Cuts at: 6148 6235 6242 6470 1391
Size: 87 7 228 4072
Fragments arranged by size:
4072 1324 1269 1029 560 327 228 157
87 77 14 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 3404 3718 928
Size: 971 1212 293 314 6361
Fragments arranged by size:
6361 1212 971 314 293
BbvCI CC'TCA_GC
Cuts at: 4449 4449
Size: 9151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5270 5304 7342 7376 1903
Size: 34 3333 34 2038 34 3678
Fragments arranged by size:
3678 3333 2038 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5285 7043 8253 5285
Size: 1758 1210 6183
Fragments arranged by size:
6183 1758 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9151
BfrBI ATG'CAT
Cuts at: 4396 4396
Size: 9151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3981 4984 5605
Size: 107 122 71 631 605 2309 1003 621
Cuts at: 5605 5675 7762 136
Size: 70 2087 1525
Fragments arranged by size:
2309 2087 1525 1003 631 621 605 122
107 71 70
BglII A'GATC_T
Cuts at: 2328 9146 2328
Size: 6818 2333
BlpI GC'TnA_GC
Cuts at: 3879 4707 5721 6568 3879
Size: 828 1014 847 6462
Fragments arranged by size:
6462 1014 847 828
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2761 2909 3303 3804 4192 5445 7212
Size: 258 713 148 394 501 388 1253 1767
Cuts at: 7212 8368 1790
Size: 1156 2573
Fragments arranged by size:
2573 1767 1253 1156 713 501 394 388
258 148
BmgBI CAC'GTC
Cuts at: 1318 3109 1318
Size: 1791 7360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2755 5304 5463
Size: 416 53 83 399 24 1338 2549 159
Cuts at: 5463 6882 7840 442
Size: 1419 958 1753
Fragments arranged by size:
2549 1753 1419 1338 958 416 399 159
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5495 1056
Size: 4439 4712
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5065 5782 6162 5065
Size: 717 380 8054
Fragments arranged by size:
8054 717 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2920 3100 3379 4214 4663 4787 5056
Size: 1010 671 180 279 835 449 124 269
Cuts at: 5056 5867 5871 5927 6002 6054 6119 6170 7811
Size: 811 4 56 75 52 65 51 1641
Cuts at: 7811 1239
Size: 2579
Fragments arranged by size:
2579 1641 1010 835 811 671 449 279
269 180 124 75 65 56 52 51
4
Bpu10I CC'TnA_GC
Cuts at: 2589 3465 4184 4449 6241 2589
Size: 876 719 265 1792 5499
Fragments arranged by size:
5499 1792 876 719 265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2722 4269 4385 5048 5111 6032 7276 8141
Size: 47 1547 116 663 63 921 1244 865
Cuts at: 8141 8439 2675
Size: 298 3387
Fragments arranged by size:
3387 1547 1244 921 865 663 298 116
63 47
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5211 7814 882
Size: 617 3712 2603 2219
Fragments arranged by size:
3712 2603 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3313 3942 6942 493
Size: 711 2109 629 3000 2702
Fragments arranged by size:
3000 2702 2109 711 629
BsaBI GATnn'nnATC
Cuts at: 3783 9145 3783
Size: 5362 3789
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 3838 3867 6958
Size: 53 83 186 936 2149 156 29 3091
Cuts at: 6958 7340 7975 275
Size: 382 635 1451
Fragments arranged by size:
3091 2149 1451 936 635 382 186 156
83 53 29
BsaWI w'CCGG_w
Cuts at: 2038 4044 7584 7982 8256 8403 8814 2038
Size: 2006 3540 398 274 147 411 2375
Fragments arranged by size:
3540 2375 2006 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3191 3221 4026 4056 5467
Size: 30 165 30 1788 30 805 30 1411
Cuts at: 5467 5497 5847 5877 5900 5930 6206 6236 1178
Size: 30 350 30 23 30 276 30 4093
Fragments arranged by size:
4093 1788 1411 805 350 276 165 30
30 30 30 30 30 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2193 2287 2519 3212 3455 3752 3845 4228
Size: 1007 94 232 693 243 297 93 383
Cuts at: 4228 5441 5774 5999 1186
Size: 1213 333 225 4338
Fragments arranged by size:
4338 1213 1007 693 383 333 297 243
232 225 94 93
BseYI C'CCAG_C
Cuts at: 2064 4525 4740 5161 5191 5378 8354 8850 2064
Size: 2461 215 421 30 187 2976 496 2365
Fragments arranged by size:
2976 2461 2365 496 421 215 187 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 2701 3591 5130 5187 1163
Size: 573 720 245 890 1539 57 5127
Fragments arranged by size:
5127 1539 890 720 573 245 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6601 7362 8390 1540
Size: 275 186 4600 761 1028 2301
Fragments arranged by size:
4600 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3744 3804 4025 4071 5991 6242 7212 7297 8368
Size: 60 221 46 1920 251 970 85 1071
Cuts at: 8368 3744
Size: 4527
Fragments arranged by size:
4527 1920 1071 970 251 221 85 60
46
BsmI GAATG_Cn'
Cuts at: 6662 6755 6662
Size: 93 9058
BsmBI CGTCTCn'nnnn_
Cuts at: 3764 3764
Size: 9151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2715 2761 2909 3303 3744 3804
Size: 399 258 667 46 148 394 441 60
Cuts at: 3804 4025 4071 4192 4631 5445 5900 5977 5991
Size: 221 46 121 439 814 455 77 14
Cuts at: 5991 6148 6235 6242 6470 7212 7297 8368 1391
Size: 157 87 7 228 742 85 1071 2174
Fragments arranged by size:
2174 1071 814 742 667 455 441 439
399 394 258 228 221 157 148 121
87 85 77 60 46 46 14 7
BspEI T'CCGG_A
Cuts at: 8814 8814
Size: 9151
BspHI T'CATG_A
Cuts at: 3513 5991 7040 8770 3513
Size: 2478 1049 1730 3894
Fragments arranged by size:
3894 2478 1730 1049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3682 4555 4848 4983 5005 5359 6015 6506
Size: 2838 873 293 135 22 354 656 491
Cuts at: 6506 844
Size: 3489
Fragments arranged by size:
3489 2838 873 656 491 354 293 135
22
BsrBI CCG'CTC
Cuts at: 1394 1823 2323 7038 1394
Size: 429 500 4715 3507
Fragments arranged by size:
4715 3507 500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2094 2363 2684 4586 4728 7646 7820
Size: 1183 846 269 321 1902 142 2918 174
Cuts at: 7820 65
Size: 1396
Fragments arranged by size:
2918 1902 1396 1183 846 321 269 174
142
BsrFI r'CCGG_y
Cuts at: 1700 1810 3705 5142 7795 7982 1700
Size: 110 1895 1437 2653 187 2869
Fragments arranged by size:
2869 2653 1895 1437 187 110
BsrGI T'GTAC_A
Cuts at: 96 3040 96
Size: 2944 6207
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9151
BssSI C'ACGA_G
Cuts at: 3597 3734 4206 4455 7211 8223 3597
Size: 137 472 249 2756 1012 4525
Fragments arranged by size:
4525 2756 1012 472 249 137
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3930 5596 6052 794
Size: 3136 1666 456 3893
Fragments arranged by size:
3893 3136 1666 456
BstBI TT'CG_AA
Cuts at: 2740 7955 2740
Size: 5215 3936
BstEII G'GTnAC_C
Cuts at: 1184 4919 6016 1184
Size: 3735 1097 4319
Fragments arranged by size:
4319 3735 1097
BstXI CCAn_nnnn'nTGG
Cuts at: 2569 5317 2569
Size: 2748 6403
BstYI r'GATC_y
Cuts at: 1972 2328 3778 4908 6481 6848 7233 7250 8702
Size: 356 1450 1130 1573 367 385 17 1452
Cuts at: 8702 8788 8800 9146 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1573 1452 1450 1130 385 367 356
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3195 5613 5961 3195
Size: 2418 348 6385
Fragments arranged by size:
6385 2418 348
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2797 3538 5422 513
Size: 552 939 147 646 741 1884 4242
Fragments arranged by size:
4242 1884 939 741 646 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8412
Fragments arranged by size:
8412 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 3160 4472 5084 5402 5435 5486 5784 5818
Size: 399 1312 612 318 33 51 298 34
Cuts at: 5818 6667 7461 7481 2761
Size: 849 794 20 4431
Fragments arranged by size:
4431 1312 849 794 612 399 318 298
51 34 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9116
DraI TTT'AAA
Cuts at: 2304 6455 2304
Size: 4151 5000
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8158 809
Size: 507 6842 1802
Fragments arranged by size:
6842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4159 4314 4467
Size: 54 1425 50 275 18 2329 155 153
Cuts at: 4467 5549 6598 7487 9116 8
Size: 1082 1049 889 1629 43
Fragments arranged by size:
2329 1629 1425 1082 1049 889 275 155
153 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6598 1537
Size: 275 4786 4090
Fragments arranged by size:
4786 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4173 4986 5344 6391 7081 1353
Size: 2820 813 358 1047 690 3423
Fragments arranged by size:
3423 2820 1047 813 690 358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2132 2939 7738 8112 8258 387
Size: 1064 138 543 807 4799 374 146 1280
Fragments arranged by size:
4799 1280 1064 807 543 374 146 138
EcoICRI GAG'CTC
Cuts at: 3742 4069 5989 6240 3742
Size: 327 1920 251 6653
Fragments arranged by size:
6653 1920 327 251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2920 2964 3100 3379 3660 4112
Size: 393 617 671 44 136 279 281 452
Cuts at: 4112 4214 4223 4268 4541 4563 4663 4787 5056
Size: 102 9 45 273 22 100 124 269
Cuts at: 5056 5612 5709 5763 5867 5871 5927 6002 6054
Size: 556 97 54 104 4 56 75 52
Cuts at: 6054 6119 6170 6203 6372 7214 7811 8598 8866
Size: 65 51 33 169 842 597 787 268
Cuts at: 8866 1239
Size: 1524
Fragments arranged by size:
1524 842 787 671 617 597 556 452
393 281 279 273 269 268 169 136
124 104 102 100 97 75 65 56
54 52 51 45 44 33 22 9
4
EcoNI CCTnn'n_nnAGG
Cuts at: 4262 4427 5408 6011 4262
Size: 165 981 603 7402
Fragments arranged by size:
7402 981 603 165
EcoO109I rG'GnC_Cy
Cuts at: 2558 3850 4357 4603 4951 4972 5209 5623 6004
Size: 1292 507 246 348 21 237 414 381
Cuts at: 6004 2558
Size: 5705
Fragments arranged by size:
5705 1292 507 414 381 348 246 237
21
EcoRI G'AATT_C
Cuts at: 2072 3074 5071 6084 6460 6838 2072
Size: 1002 1997 1013 376 378 4385
Fragments arranged by size:
4385 1997 1013 1002 378 376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2102 2716 2102
Size: 614 8537
FspI TGC'GCA
Cuts at: 7657 7657
Size: 9151
HaeII r_GCGC'y
Cuts at: 1678 3718 4741 5162 8298 1678
Size: 2040 1023 421 3136 2531
Fragments arranged by size:
3136 2531 2040 1023 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3763 3795 4026 4058 4312
Size: 32 135 32 2191 32 231 32 254
Cuts at: 4312 4344 5105 5137 5210 5242 5900 5932 6206
Size: 32 761 32 73 32 658 32 274
Cuts at: 6206 6238 6270 6368 6400 7792 7824 7866 7898
Size: 32 32 98 32 1392 32 42 32
Cuts at: 7898 1373
Size: 2626
Fragments arranged by size:
2626 2191 1392 761 658 274 254 231
135 98 73 42 32 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 669 1657 1938 2185 2465 3649 5937 6495 6741
Size: 988 281 247 280 1184 2288 558 246
Cuts at: 6741 669
Size: 3079
Fragments arranged by size:
3079 2288 1184 988 558 281 280 247
246
HindIII A'AGCT_T
Cuts at: 748 2816 2946 4884 748
Size: 2068 130 1938 5015
Fragments arranged by size:
5015 2068 1938 130
HpaI GTT'AAC
Cuts at: 6741 6741
Size: 9151
KpnI G_GTAC'C
Cuts at: 6476 6476
Size: 9151
MfeI C'AATT_G
Cuts at: 6750 6750
Size: 9151
MluI A'CGCG_T
Cuts at: 9027 9027
Size: 9151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3045 3203 3756 4488 5369 5931 6110 8265
Size: 1740 158 553 732 881 562 179 2155
Cuts at: 8265 8449 1305
Size: 184 2007
Fragments arranged by size:
2155 2007 1740 881 732 562 553 184
179 158
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4316 4469 10
Size: 54 1425 343 2484 153 4692
Fragments arranged by size:
4692 2484 1425 343 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2041 2754 3273 3296 3387 3512 3530
Size: 648 875 713 519 23 91 125 18
Cuts at: 3530 4005 7109 7468 7627 518
Size: 475 3104 359 159 2042
Fragments arranged by size:
3104 2042 875 713 648 519 475 359
159 125 91 23 18
MspA1I CmG'CkG
Cuts at: 1606 2153 4336 4525 5284 5378 5716 6049 6392
Size: 547 2183 189 759 94 338 333 343
Cuts at: 6392 6567 7244 8392 8637 1606
Size: 175 677 1148 245 2120
Fragments arranged by size:
2183 2120 1148 759 677 547 343 338
333 245 189 175 94
NaeI GCC'GGC
Cuts at: 3707 3707
Size: 9151
NcoI C'CATG_G
Cuts at: 513 1065 2004 3538 5422 513
Size: 552 939 1534 1884 4242
Fragments arranged by size:
4242 1884 1534 939 552
NdeI CA'TA_TG
Cuts at: 387 3045 387
Size: 2658 6493
NgoMIV G'CCGG_C
Cuts at: 3705 3705
Size: 9151
NheI G'CTAG_C
Cuts at: 1052 5491 1052
Size: 4439 4712
NotI GC'GGCC_GC
Cuts at: 6598 6598
Size: 9151
NsiI A_TGCA'T
Cuts at: 4398 4398
Size: 9151
NspI r_CATG'y
Cuts at: 1132 1229 2628 3037 6509 8054 1132
Size: 97 1399 409 3472 1545 2229
Fragments arranged by size:
3472 2229 1545 1399 409 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9151
PciI A'CATG_T
Cuts at: 8050 8050
Size: 9151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3222 5655 1108
Size: 2114 2433 4604
Fragments arranged by size:
4604 2433 2114
PfoI T'CCnGG_A
Cuts at: 4422 5033 5887 4422
Size: 611 854 7686
Fragments arranged by size:
7686 854 611
PmeI GTTT'AAAC
Cuts at: 6455 6455
Size: 9151
PmlI CAC'GTG
Cuts at: 3942 3942
Size: 9151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7220 7252 8733 8765 7220
Size: 32 1481 32 7606
Fragments arranged by size:
7606 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2558 4357 4972 5209 6004 2558
Size: 1799 615 237 795 5705
Fragments arranged by size:
5705 1799 795 615 237
PshAI GACnn'nnGTC
Cuts at: 1613 5406 1613
Size: 3793 5358
PsiI TTA'TAA
Cuts at: 6721 6721
Size: 9151
PstI C_TGCA'G
Cuts at: 830 5016 5283 5397 5592 6057 6503 830
Size: 4186 267 114 195 465 446 3478
Fragments arranged by size:
4186 3478 465 446 267 195 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9151
PvuII CAG'CTG
Cuts at: 4336 4525 5284 5378 5716 6049 6392 4336
Size: 189 759 94 338 333 343 7095
Fragments arranged by size:
7095 759 343 338 333 189 94
SacI G_AGCT'C
Cuts at: 3744 4071 5991 6242 3744
Size: 327 1920 251 6653
Fragments arranged by size:
6653 1920 327 251
SacII CC_GC'GG
Cuts at: 2154 2154
Size: 9151
SalI G'TCGA_C
Cuts at: 1936 2463 6493 1936
Size: 527 4030 4594
Fragments arranged by size:
4594 4030 527
SapI GCTCTTCn'nnn_
Cuts at: 4173 4986 6391 4173
Size: 813 1405 6933
Fragments arranged by size:
6933 1405 813
SbfI CC_TGCA'GG
Cuts at: 6503 6503
Size: 9151
ScaI AGT'ACT
Cuts at: 7399 9111 7399
Size: 1712 7439
SexAI A'CCwGG_T
Cuts at: 3401 3401
Size: 9151
SfcI C'TryA_G
Cuts at: 826 1045 2341 2458 2480 2499 3054 5012 5279
Size: 219 1296 117 22 19 555 1958 267
Cuts at: 5279 5393 5588 5738 6053 6499 7634 8315 8506
Size: 114 195 150 315 446 1135 681 191
Cuts at: 8506 826
Size: 1471
Fragments arranged by size:
1958 1471 1296 1135 681 555 446 315
267 219 195 191 150 117 114 22
19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9151
SmaI CCC'GGG
Cuts at: 1872 4703 6478 1872
Size: 2831 1775 4545
Fragments arranged by size:
4545 2831 1775
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2690 2701 4284 4364 5063 5090
Size: 197 926 747 11 1583 80 699 27
Cuts at: 5090 6011 7255 8156 8418 820
Size: 921 1244 901 262 1553
Fragments arranged by size:
1583 1553 1244 926 921 901 747 699
262 197 80 27 11
SnaBI TAC'GTA
Cuts at: 493 6942 493
Size: 6449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9151
SphI G_CATG'C
Cuts at: 1132 6509 1132
Size: 5377 3774
SrfI GCCC'GGGC
Cuts at: 4703 4703
Size: 9151
SspI AAT'ATT
Cuts at: 5 52 5824 7075 5
Size: 47 5772 1251 2081
Fragments arranged by size:
5772 2081 1251 47
StuI AGG'CCT
Cuts at: 1517 4439 5048 5765 1517
Size: 2922 609 717 4903
Fragments arranged by size:
4903 2922 717 609
StyI C'CwwG_G
Cuts at: 513 1065 2004 3538 3622 4059 4143 5422 5836
Size: 552 939 1534 84 437 84 1279 414
Cuts at: 5836 6590 513
Size: 754 3074
Fragments arranged by size:
3074 1534 1279 939 754 552 437 414
84 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4517 4652 7178 7195 7348 7533 7978
Size: 1255 2937 135 2526 17 153 185 445
Cuts at: 7978 325
Size: 1498
Fragments arranged by size:
2937 2526 1498 1255 445 185 153 135
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2976 3040 3656
Size: 274 80 33 51 1443 999 64 616
Cuts at: 3656 7397 9109 96
Size: 3741 1712 138
Fragments arranged by size:
3741 1712 1443 999 616 274 138 80
64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 5467 5499 5847 5879 9111 9143 2000
Size: 32 3435 32 348 32 3232 32 2008
Fragments arranged by size:
3435 3232 2008 348 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9151
XbaI T'CTAG_A
Cuts at: 4936 6487 4936
Size: 1551 7600
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2084 2154 4687 5493 5677 2084
Size: 70 2533 806 184 5558
Fragments arranged by size:
5558 2533 806 184 70
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9151
XmaI C'CCGG_G
Cuts at: 1870 4701 6476 1870
Size: 2831 1775 4545
Fragments arranged by size:
4545 2831 1775
XmnI GAAnn'nnTTC
Cuts at: 3074 7280 3074
Size: 4206 4945
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 6959 276
Size: 53 83 186 936 2305 3120 2468
Fragments arranged by size:
3120 2468 2305 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII
EcoRV FseI FspAI KasI NarI NruI PacI PspOMI
PspXI PsrI RsrII SanDI SfiI SfoI SwaI