(Circular) (Six-base) MAPSORT of: pFN21AA0233.seq Check: 3060 from: 1 to: 12268 pFN21AA0233 12268 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4312 4402 4809 5335 4312 Size: 90 407 526 11245 Fragments arranged by size: 11245 526 407 90 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4398 7884 10078 278 Size: 53 83 186 936 2862 3486 2194 2468 Fragments arranged by size: 3486 2862 2468 2194 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2103 2670 3801 3813 3855 3936 7615 7914 Size: 166 567 1131 12 42 81 3679 299 Cuts at: 7914 9611 1937 Size: 1697 4594 Fragments arranged by size: 4594 3679 1697 1131 567 299 166 81 42 12 Acc65I G'GTAC_C Cuts at: 8002 9589 8002 Size: 1587 10681 AclI AA'CG_TT Cuts at: 1563 10395 10768 1563 Size: 8832 373 3063 Fragments arranged by size: 8832 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2068 2360 2722 3641 4350 4505 5498 5749 Size: 436 292 362 919 709 155 993 251 Cuts at: 5749 6324 9364 10331 11715 11983 1632 Size: 575 3040 967 1384 268 1917 Fragments arranged by size: 3040 1917 1384 993 967 919 709 575 436 362 292 268 251 155 AfeI AGC'GCT Cuts at: 6644 7187 6644 Size: 543 11725 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 12071 AflIII A'CryG_T Cuts at: 1203 3450 3816 4143 4405 9336 11167 12144 1203 Size: 2247 366 327 262 4931 1831 977 1327 Fragments arranged by size: 4931 2247 1831 1327 977 366 327 262 AgeI A'CCGG_T Cuts at: 11099 11099 Size: 12268 AhdI GACnn_n'nnGTC Cuts at: 7978 10997 7978 Size: 3019 9249 AleI CACnn'nnGTG Cuts at: 1166 4322 1166 Size: 3156 9112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4076 4706 5627 8099 9272 9383 1184 Size: 2892 630 921 2472 1173 111 4069 Fragments arranged by size: 4069 2892 2472 1173 921 630 111 AlwNI CAG_nnn'CTG Cuts at: 2275 2295 2748 2824 3694 3706 4659 4717 4927 Size: 20 453 76 870 12 953 58 210 Cuts at: 4927 4939 5164 5874 6544 6925 7405 8758 10011 Size: 12 225 710 670 381 480 1353 1253 Cuts at: 10011 11583 2275 Size: 1572 2960 Fragments arranged by size: 2960 1572 1353 1253 953 870 710 670 480 453 381 225 210 76 58 20 12 12 ApaI G_GGCC'C Cuts at: 3497 4734 5284 5635 6143 6171 6482 7646 9102 Size: 1237 550 351 508 28 311 1164 1456 Cuts at: 9102 3497 Size: 6663 Fragments arranged by size: 6663 1456 1237 1164 550 508 351 311 28 ApaLI G'TGCA_C Cuts at: 3158 6683 10325 11481 3158 Size: 3525 3642 1156 3945 Fragments arranged by size: 3945 3642 3525 1156 ApoI r'AATT_y Cuts at: 1493 4982 9577 9803 9955 12064 1493 Size: 3489 4595 226 152 2109 1697 Fragments arranged by size: 4595 3489 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 10822 160 Size: 872 9790 1606 Fragments arranged by size: 9790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12268 AvaI C'yCGr_G Cuts at: 1870 1943 2730 3054 3221 6310 6482 6887 7171 Size: 73 787 324 167 3089 172 405 284 Cuts at: 7171 8006 9029 9474 9593 1870 Size: 835 1023 445 119 4545 Fragments arranged by size: 4545 3089 1023 835 787 445 405 324 284 172 167 119 73 BamHI G'GATC_C Cuts at: 9598 9965 9598 Size: 367 11901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2424 2771 3118 3554 4060 Size: 325 217 627 637 347 347 436 506 Cuts at: 4060 4324 4845 4913 5091 6606 6614 6905 6960 Size: 264 521 68 178 1515 8 291 55 Cuts at: 6960 7501 8002 8338 9035 9589 11044 618 Size: 541 501 336 697 554 1455 1842 Fragments arranged by size: 1842 1515 1455 697 637 627 554 541 521 506 501 436 347 347 336 325 291 264 217 178 68 55 8 BanII G_rGCy'C Cuts at: 1391 2453 3182 3222 3497 3678 4044 4180 4671 Size: 1062 729 40 275 181 366 136 491 Cuts at: 4671 4734 5284 5430 5635 6143 6171 6188 6482 Size: 63 550 146 205 508 28 17 294 Cuts at: 6482 6666 7587 7646 8880 9102 9587 1391 Size: 184 921 59 1234 222 485 4072 Fragments arranged by size: 4072 1234 1062 921 729 550 508 491 485 366 294 275 222 205 184 181 146 136 63 59 40 28 17 BbeI G_GCGC'C Cuts at: 2428 2775 4849 6964 2428 Size: 347 2074 2115 7732 Fragments arranged by size: 7732 2115 2074 347 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5482 5710 5899 6312 7138 7201 7343 Size: 971 3583 228 189 413 826 63 142 Cuts at: 7343 7527 928 Size: 184 5669 Fragments arranged by size: 5669 3583 971 826 413 228 189 184 142 63 BbvCI CC'TCA_GC Cuts at: 2201 6380 7677 2201 Size: 4179 1297 6792 Fragments arranged by size: 6792 4179 1297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3751 3785 7868 7902 8206 8240 10459 Size: 34 1814 34 4083 34 304 34 2219 Cuts at: 10459 10493 1903 Size: 34 3678 Fragments arranged by size: 4083 3678 2219 1814 304 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6083 9173 10160 11370 6083 Size: 3090 987 1210 6981 Fragments arranged by size: 6981 3090 1210 987 BclI T'GATC_A Cuts at: 1548 9543 1548 Size: 7995 4273 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3018 3202 6202 Size: 107 122 71 631 605 1346 184 3000 Cuts at: 6202 7831 8494 10879 136 Size: 1629 663 2385 1525 Fragments arranged by size: 3000 2385 1629 1525 1346 663 631 605 184 122 107 71 BglII A'GATC_T Cuts at: 2238 4995 9387 12263 2238 Size: 2757 4392 2876 2243 Fragments arranged by size: 4392 2876 2757 2243 BlpI GC'TnA_GC Cuts at: 3216 4359 5358 6527 9685 3216 Size: 1143 999 1169 3158 5799 Fragments arranged by size: 5799 3158 1169 1143 999 Bme1580I G_kGCm'C Cuts at: 1790 2259 3123 3162 3497 4065 4734 5284 5442 Size: 469 864 39 335 568 669 550 158 Cuts at: 5442 5635 6143 6171 6482 6687 7646 7817 8863 Size: 193 508 28 311 205 959 171 1046 Cuts at: 8863 9038 9091 9102 10329 11485 1790 Size: 175 53 11 1227 1156 2573 Fragments arranged by size: 2573 1227 1156 1046 959 864 669 568 550 508 469 335 311 205 193 175 171 158 53 39 28 11 BmgBI CAC'GTC Cuts at: 1318 5932 6628 7980 1318 Size: 4614 696 1352 5606 Fragments arranged by size: 5606 4614 1352 696 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 8383 9999 10957 Size: 416 53 83 399 24 6966 1616 958 Cuts at: 10957 442 Size: 1753 Fragments arranged by size: 6966 1753 1616 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3071 5966 1056 Size: 2015 2895 7358 Fragments arranged by size: 7358 2895 2015 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2323 4063 4291 7453 8839 9055 2323 Size: 1740 228 3162 1386 216 5536 Fragments arranged by size: 5536 3162 1740 1386 228 216 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2647 2878 3083 3542 3621 4033 4040 Size: 980 428 231 205 459 79 412 7 Cuts at: 4040 4261 4391 4454 5012 5236 5429 5930 5953 Size: 221 130 63 558 224 193 501 23 Cuts at: 5953 6179 7001 7352 7773 9503 9509 10928 1239 Size: 226 822 351 421 1730 6 1419 2579 Fragments arranged by size: 2579 1730 1419 980 822 558 501 459 428 421 412 351 231 226 224 221 205 193 130 79 63 23 7 6 Bpu10I CC'TnA_GC Cuts at: 2201 3474 5912 6380 6836 7586 7677 2201 Size: 1273 2438 468 456 750 91 6792 Fragments arranged by size: 6792 2438 1273 750 468 456 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2849 3866 4610 4937 5987 8630 9422 10393 11258 Size: 1017 744 327 1050 2643 792 971 865 Cuts at: 11258 11556 2849 Size: 298 3561 Fragments arranged by size: 3561 2643 1050 1017 971 865 792 744 327 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2294 2401 2867 5814 9530 10931 882 Size: 617 795 107 466 2947 3716 1401 2219 Fragments arranged by size: 3716 2947 2219 1401 795 617 466 107 BsaAI yAC'GTr Cuts at: 493 1204 2017 3115 3451 4144 7276 9337 10059 Size: 711 813 1098 336 693 3132 2061 722 Cuts at: 10059 493 Size: 2702 Fragments arranged by size: 3132 2702 2061 1098 813 722 711 693 336 BsaBI GATnn'nnATC Cuts at: 12262 12262 Size: 12268 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2425 2772 2955 3230 Size: 53 83 186 936 892 347 183 275 Cuts at: 3230 4395 4846 5391 6961 7881 10075 10457 11092 Size: 1165 451 545 1570 920 2194 382 635 Cuts at: 11092 275 Size: 1451 Fragments arranged by size: 2194 1570 1451 1165 936 920 892 635 545 451 382 347 275 186 183 83 53 BsaWI w'CCGG_w Cuts at: 9532 10701 11099 11373 11520 11931 9532 Size: 1169 398 274 147 411 9869 Fragments arranged by size: 9869 1169 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2999 3029 4130 4160 6648 Size: 30 165 30 1596 30 1101 30 2488 Cuts at: 6648 6678 7746 7776 8937 8967 9404 9434 9482 Size: 30 1068 30 1161 30 437 30 48 Cuts at: 9482 9485 9512 9515 1178 Size: 3 27 3 3931 Fragments arranged by size: 3931 2488 1596 1161 1101 1068 437 165 48 30 30 30 30 30 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2389 4235 4268 4271 4274 4277 4280 4283 Size: 1203 1846 33 3 3 3 3 3 Cuts at: 4283 5384 5861 6047 6178 6302 6305 6500 6641 Size: 1101 477 186 131 124 3 195 141 Cuts at: 6641 6809 6884 6998 7445 7484 7487 7502 8741 Size: 168 75 114 447 39 3 15 1239 Cuts at: 8741 9152 9425 9506 9509 1186 Size: 411 273 81 3 3945 Fragments arranged by size: 3945 1846 1239 1203 1101 477 447 411 273 195 186 168 141 131 124 114 81 75 39 33 15 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2194 2282 2295 2600 2857 3514 4003 4173 4291 Size: 88 13 305 257 657 489 170 118 Cuts at: 4291 5334 5563 5748 6048 6452 6553 6784 7178 Size: 1043 229 185 300 404 101 231 394 Cuts at: 7178 7398 7645 8086 8193 8650 8685 8751 8852 Size: 220 247 441 107 457 35 66 101 Cuts at: 8852 11471 11967 2194 Size: 2619 496 2495 Fragments arranged by size: 2619 2495 1043 657 496 489 457 441 404 394 305 300 257 247 231 229 220 185 170 118 107 101 101 88 66 35 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2680 4082 4430 4645 5039 5912 6536 Size: 573 944 1402 348 215 394 873 624 Cuts at: 6536 7388 7838 8988 1163 Size: 852 450 1150 4443 Fragments arranged by size: 4443 1402 1150 944 873 852 624 573 450 394 348 215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3790 5404 7773 7826 8895 9718 Size: 275 186 1789 1614 2369 53 1069 823 Cuts at: 9718 10479 11507 1540 Size: 761 1028 2301 Fragments arranged by size: 2369 2301 1789 1614 1069 1028 823 761 275 186 53 BsiHKAI G_wGCw'C Cuts at: 2022 3162 3182 3404 3528 4044 4143 4857 6533 Size: 1140 20 222 124 516 99 714 1676 Cuts at: 6533 6666 6687 6842 7143 7587 8880 10329 10414 Size: 133 21 155 301 444 1293 1449 85 Cuts at: 10414 11485 2022 Size: 1071 2805 Fragments arranged by size: 2805 1676 1449 1293 1140 1071 714 516 444 301 222 155 133 124 99 85 21 20 BsmI GAATG_Cn' Cuts at: 9779 9872 9779 Size: 93 12175 BsmBI CGTCTCn'nnnn_ Cuts at: 3237 3237 Size: 12268 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2259 2453 3123 3162 3182 3222 Size: 399 232 237 194 670 39 20 40 Cuts at: 3222 3404 3497 3528 3678 4044 4065 4143 4180 Size: 182 93 31 150 366 21 78 37 Cuts at: 4180 4671 4734 4857 5284 5430 5442 5635 6143 Size: 491 63 123 427 146 12 193 508 Cuts at: 6143 6171 6188 6482 6533 6666 6687 6842 7143 Size: 28 17 294 51 133 21 155 301 Cuts at: 7143 7587 7646 7817 8863 8880 9038 9091 9102 Size: 444 59 171 1046 17 158 53 11 Cuts at: 9102 9587 10329 10414 11485 1391 Size: 485 742 85 1071 2174 Fragments arranged by size: 2174 1071 1046 742 670 508 491 485 444 427 399 366 301 294 237 232 194 193 182 171 158 155 150 146 133 123 93 85 78 63 59 53 51 40 39 37 31 28 21 21 20 17 17 12 11 BspEI T'CCGG_A Cuts at: 11931 11931 Size: 12268 BspHI T'CATG_A Cuts at: 10157 11887 10157 Size: 1730 10538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 2682 3474 4312 4402 4515 4598 4809 Size: 1271 567 792 838 90 113 83 211 Cuts at: 4809 5172 5271 5335 5448 5580 5598 5934 8079 Size: 363 99 64 113 132 18 336 2145 Cuts at: 8079 9261 9623 844 Size: 1182 362 3489 Fragments arranged by size: 3489 2145 1271 1182 838 792 567 363 362 336 211 132 113 113 99 90 83 64 18 BsrBI CCG'CTC Cuts at: 1394 1823 2561 3238 4363 7395 7464 7661 7749 Size: 429 738 677 1125 3032 69 197 88 Cuts at: 7749 9528 10155 1394 Size: 1779 627 3507 Fragments arranged by size: 3507 3032 1779 1125 738 677 627 429 197 88 69 BsrDI GCAATG_nn' Cuts at: 65 1248 2189 3288 5103 10763 10937 65 Size: 1183 941 1099 1815 5660 174 1396 Fragments arranged by size: 5660 1815 1396 1183 1099 941 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2774 2837 2921 3170 3785 4382 4842 Size: 110 964 63 84 249 615 597 460 Cuts at: 4842 4863 5738 6842 7118 9197 10912 11099 1700 Size: 21 875 1104 276 2079 1715 187 2869 Fragments arranged by size: 2869 2079 1715 1104 964 875 615 597 460 276 249 187 110 84 63 21 BsrGI T'GTAC_A Cuts at: 96 4464 5664 6708 96 Size: 4368 1200 1044 5656 Fragments arranged by size: 5656 4368 1200 1044 BssHII G'CGCG_C Cuts at: 1925 2055 3282 1925 Size: 130 1227 10911 Fragments arranged by size: 10911 1227 130 BssSI C'ACGA_G Cuts at: 3161 3794 3899 5639 9555 10328 11340 3161 Size: 633 105 1740 3916 773 1012 4089 Fragments arranged by size: 4089 3916 1740 1012 773 633 105 BstAPI GCAn_nnn'nTGC Cuts at: 794 2254 2748 3118 4810 6329 6523 794 Size: 1460 494 370 1692 1519 194 6539 Fragments arranged by size: 6539 1692 1519 1460 494 370 194 BstBI TT'CG_AA Cuts at: 11072 11072 Size: 12268 BstEII G'GTnAC_C Cuts at: 1184 3655 5047 6358 1184 Size: 2471 1392 1311 7094 Fragments arranged by size: 7094 2471 1392 1311 BstXI CCAn_nnnn'nTGG Cuts at: 2365 3490 4057 6784 9349 2365 Size: 1125 567 2727 2565 5284 Fragments arranged by size: 5284 2727 2565 1125 567 BstYI r'GATC_y Cuts at: 1972 2238 3744 4936 4995 8244 9387 9598 9965 Size: 266 1506 1192 59 3249 1143 211 367 Cuts at: 9965 10350 10367 11819 11905 11917 12263 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 3249 1977 1506 1452 1192 1143 385 367 346 266 211 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 8790 8790 Size: 12268 BtgI C'CryG_G Cuts at: 513 1065 2004 2459 2754 2979 3459 3660 4560 Size: 552 939 455 295 225 480 201 900 Cuts at: 4560 4698 4734 6197 6610 7554 7722 8046 8601 Size: 138 36 1463 413 944 168 324 555 Cuts at: 8601 9261 513 Size: 660 3520 Fragments arranged by size: 3520 1463 944 939 900 660 555 552 480 455 413 324 295 225 201 168 138 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2626 3913 8539 487 Size: 683 56 1400 1287 4626 4216 Fragments arranged by size: 4626 4216 1400 1287 683 56 BtsI GCAGTG_nn' Cuts at: 2621 4200 4208 5345 5452 6212 6865 6871 8370 Size: 1579 8 1137 107 760 653 6 1499 Cuts at: 8370 9000 9784 10578 10598 2621 Size: 630 784 794 20 4291 Fragments arranged by size: 4291 1579 1499 1137 794 784 760 653 630 107 20 8 6 ClaI AT'CG_AT Cuts at: 1554 8628 1554 Size: 7074 5194 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12233 DraI TTT'AAA Cuts at: 9572 9572 Size: 12268 DraIII CAC_nnn'GTG Cuts at: 2703 3118 4584 6329 7279 2703 Size: 415 1466 1745 950 7692 Fragments arranged by size: 7692 1745 1466 950 415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 11275 809 Size: 507 9959 1802 Fragments arranged by size: 9959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2556 2613 2776 Size: 54 1425 50 275 18 726 57 163 Cuts at: 2776 3019 3172 3390 3787 4299 5010 5073 5484 Size: 243 153 218 397 512 711 63 411 Cuts at: 5484 6936 7053 7200 7509 7770 7823 7965 8121 Size: 1452 117 147 309 261 53 142 156 Cuts at: 8121 8760 8892 9018 9715 10604 12233 8 Size: 639 132 126 697 889 1629 43 Fragments arranged by size: 1629 1452 1425 889 726 711 697 639 512 411 397 309 275 261 243 218 163 156 153 147 142 132 126 117 63 57 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3787 7770 7823 8892 9715 1537 Size: 275 1975 3983 53 1069 823 4090 Fragments arranged by size: 4090 3983 1975 1069 823 275 53 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2216 2687 3830 3851 4049 5138 5603 Size: 764 99 471 1143 21 198 1089 465 Cuts at: 5603 5768 6279 7689 7701 7730 7753 8318 8324 Size: 165 511 1410 12 29 23 565 6 Cuts at: 8324 8552 8585 9074 10198 1353 Size: 228 33 489 1124 3423 Fragments arranged by size: 3423 1410 1143 1124 1089 764 565 511 489 471 465 228 198 165 99 33 29 23 21 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3886 7105 8224 10855 11229 11375 Size: 1064 138 2297 3219 1119 2631 374 146 Cuts at: 11375 387 Size: 1280 Fragments arranged by size: 3219 2631 2297 1280 1119 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3180 4042 6664 7585 8878 3180 Size: 862 2622 921 1293 6570 Fragments arranged by size: 6570 2622 1293 921 862 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2219 2360 2647 2722 2878 3083 Size: 393 436 151 141 287 75 156 205 Cuts at: 3083 3542 3621 3641 4033 4040 4261 4350 4391 Size: 459 79 20 392 7 221 89 41 Cuts at: 4391 4454 4505 5012 5236 5429 5498 5749 5930 Size: 63 51 507 224 193 69 251 181 Cuts at: 5930 5953 6179 6324 7001 7352 7773 9364 9503 Size: 23 226 145 677 351 421 1591 139 Cuts at: 9503 9509 10331 10928 11715 11983 1239 Size: 6 822 597 787 268 1524 Fragments arranged by size: 1591 1524 822 787 677 597 507 459 436 421 393 392 351 287 268 251 226 224 221 205 193 181 156 151 145 141 139 89 79 75 69 63 51 41 23 20 7 6 EcoNI CCTnn'n_nnAGG Cuts at: 3848 4487 6656 7808 8321 8939 9029 3848 Size: 639 2169 1152 513 618 90 7087 Fragments arranged by size: 7087 2169 1152 639 618 513 90 EcoO109I rG'GnC_Cy Cuts at: 2279 2608 2879 3494 4009 4113 4203 4348 4730 Size: 329 271 615 515 104 90 145 382 Cuts at: 4730 5281 5460 6140 6148 6167 6478 6479 6740 Size: 551 179 680 8 19 311 1 261 Cuts at: 6740 6751 7563 7590 7642 7717 8932 2279 Size: 11 812 27 52 75 1215 5615 Fragments arranged by size: 5615 1215 812 680 615 551 515 382 329 311 271 261 179 145 104 90 75 52 27 19 11 8 1 EcoRI G'AATT_C Cuts at: 9577 9955 9577 Size: 378 11890 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 7324 8116 9532 7324 Size: 792 1416 10060 Fragments arranged by size: 10060 1416 792 FspI TGC'GCA Cuts at: 2993 3374 6968 8075 10774 2993 Size: 381 3594 1107 2699 4487 Fragments arranged by size: 4487 3594 2699 1107 381 HaeII r_GCGC'y Cuts at: 1678 2428 2775 4849 5144 5215 5902 6566 6646 Size: 750 347 2074 295 71 687 664 80 Cuts at: 6646 6964 7022 7189 7291 7837 8114 8685 8834 Size: 318 58 167 102 546 277 571 149 Cuts at: 8834 11415 1678 Size: 2581 2531 Fragments arranged by size: 2581 2531 2074 750 687 664 571 546 347 318 295 277 167 149 102 80 71 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2142 2174 2440 2472 4130 Size: 32 135 32 570 32 266 32 1658 Cuts at: 4130 4162 4385 4417 6023 6055 7427 7459 7964 Size: 32 223 32 1606 32 1372 32 505 Cuts at: 7964 7996 8717 8749 10909 10941 10983 11015 1373 Size: 32 721 32 2160 32 42 32 2626 Fragments arranged by size: 2626 2160 1658 1606 1372 721 570 505 266 223 135 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4636 5395 7915 8056 8617 9612 Size: 988 281 2698 759 2520 141 561 995 Cuts at: 9612 9858 669 Size: 246 3079 Fragments arranged by size: 3079 2698 2520 995 988 759 561 281 246 141 HindIII A'AGCT_T Cuts at: 748 748 Size: 12268 HpaI GTT'AAC Cuts at: 9858 9858 Size: 12268 KasI G'GCGC_C Cuts at: 2424 2771 4845 6960 2424 Size: 347 2074 2115 7732 Fragments arranged by size: 7732 2115 2074 347 KpnI G_GTAC'C Cuts at: 8006 9593 8006 Size: 1587 10681 MfeI C'AATT_G Cuts at: 9867 9867 Size: 12268 MluI A'CGCG_T Cuts at: 4405 12144 4405 Size: 7739 4529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6297 7528 11382 11566 1305 Size: 4992 1231 3854 184 2007 Fragments arranged by size: 4992 3854 2007 1231 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3392 4301 5075 8123 8762 Size: 54 1425 343 1560 909 774 3048 639 Cuts at: 8762 9020 10 Size: 258 3258 Fragments arranged by size: 3258 3048 1560 1425 909 774 639 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 3293 4058 4133 4322 4601 7532 Size: 648 1224 903 765 75 189 279 2931 Cuts at: 7532 10226 10585 10744 518 Size: 2694 359 159 2042 Fragments arranged by size: 2931 2694 2042 1224 903 765 648 359 279 189 159 75 MspA1I CmG'CkG Cuts at: 1606 2088 2286 2295 2756 3324 3514 3641 4003 Size: 482 198 9 461 568 190 127 362 Cuts at: 4003 4096 4876 4885 4911 4927 5357 5416 6052 Size: 93 780 9 26 16 430 59 636 Cuts at: 6052 6340 6494 6706 7402 8250 8409 8575 8668 Size: 288 154 212 696 848 159 166 93 Cuts at: 8668 8758 9016 9124 9169 9684 10361 11509 11754 Size: 90 258 108 45 515 677 1148 245 Cuts at: 11754 1606 Size: 2120 Fragments arranged by size: 2120 1148 848 780 696 677 636 568 515 482 461 430 362 288 258 245 212 198 190 166 159 154 127 108 93 93 90 59 45 26 16 9 9 NaeI GCC'GGC Cuts at: 2776 2839 3787 4384 4865 7120 2776 Size: 63 948 597 481 2255 7924 Fragments arranged by size: 7924 2255 948 597 481 63 NarI GG'CG_CC Cuts at: 2425 2772 4846 6961 2425 Size: 347 2074 2115 7732 Fragments arranged by size: 7732 2115 2074 347 NcoI C'CATG_G Cuts at: 513 1065 2004 3459 4560 8046 9261 513 Size: 552 939 1455 1101 3486 1215 3520 Fragments arranged by size: 3520 3486 1455 1215 1101 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 12268 NgoMIV G'CCGG_C Cuts at: 2774 2837 3785 4382 4863 7118 2774 Size: 63 948 597 481 2255 7924 Fragments arranged by size: 7924 2255 948 597 481 63 NheI G'CTAG_C Cuts at: 1052 3067 5962 1052 Size: 2015 2895 7358 Fragments arranged by size: 7358 2895 2015 NotI GC'GGCC_GC Cuts at: 9715 9715 Size: 12268 NruI TCG'CGA Cuts at: 2311 2311 Size: 12268 NspI r_CATG'y Cuts at: 1132 1229 3766 3820 5476 5698 8980 9626 11171 Size: 97 2537 54 1656 222 3282 646 1545 Cuts at: 11171 1132 Size: 2229 Fragments arranged by size: 3282 2537 2229 1656 1545 646 222 97 54 PasI CC'CwG_GG Cuts at: 1348 3490 6136 7810 1348 Size: 2142 2646 1674 5806 Fragments arranged by size: 5806 2646 2142 1674 PciI A'CATG_T Cuts at: 3816 11167 3816 Size: 7351 4917 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 2908 3115 3247 3388 3741 4584 4927 Size: 1167 633 207 132 141 353 843 343 Cuts at: 4927 6463 7051 8185 8565 8758 9052 1108 Size: 1536 588 1134 380 193 294 4324 Fragments arranged by size: 4324 1536 1167 1134 843 633 588 380 353 343 294 207 193 141 132 PfoI T'CCnGG_A Cuts at: 3951 6951 3951 Size: 3000 9268 PmeI GTTT'AAAC Cuts at: 9572 9572 Size: 12268 PmlI CAC'GTG Cuts at: 2017 3115 4144 7276 2017 Size: 1098 1029 3132 7009 Fragments arranged by size: 7009 3132 1098 1029 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8156 8188 8654 8686 10337 10369 11850 11882 8156 Size: 32 466 32 1651 32 1481 32 8542 Fragments arranged by size: 8542 1651 1481 466 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2279 2608 2879 4009 4203 5460 6148 7563 8932 Size: 329 271 1130 194 1257 688 1415 1369 Cuts at: 8932 2279 Size: 5615 Fragments arranged by size: 5615 1415 1369 1257 1130 688 329 271 194 PshAI GACnn'nnGTC Cuts at: 1613 3653 4592 6117 1613 Size: 2040 939 1525 7764 Fragments arranged by size: 7764 2040 1525 939 PsiI TTA'TAA Cuts at: 9838 9838 Size: 12268 PspOMI G'GGCC_C Cuts at: 3493 4730 5280 5631 6139 6167 6478 7642 9098 Size: 1237 550 351 508 28 311 1164 1456 Cuts at: 9098 3493 Size: 6663 Fragments arranged by size: 6663 1456 1237 1164 550 508 351 311 28 PspXI vC'TCGA_Gb Cuts at: 9029 9029 Size: 12268 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 7289 7321 7289 Size: 32 12236 PstI C_TGCA'G Cuts at: 830 2291 2517 4095 5459 5619 5898 6594 6672 Size: 1461 226 1578 1364 160 279 696 78 Cuts at: 6672 7788 9129 9231 9620 830 Size: 1116 1341 102 389 3478 Fragments arranged by size: 3478 1578 1461 1364 1341 1116 696 389 279 226 160 102 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12268 PvuII CAG'CTG Cuts at: 2286 3324 3514 3641 4003 4096 4876 4927 5357 Size: 1038 190 127 362 93 780 51 430 Cuts at: 5357 6052 6340 6706 7402 8409 9016 9124 9169 Size: 695 288 366 696 1007 607 108 45 Cuts at: 9169 2286 Size: 5385 Fragments arranged by size: 5385 1038 1007 780 696 695 607 430 366 362 288 190 127 108 93 51 45 RsrII CG'GwC_CG Cuts at: 9240 9240 Size: 12268 SacI G_AGCT'C Cuts at: 3182 4044 6666 7587 8880 3182 Size: 862 2622 921 1293 6570 Fragments arranged by size: 6570 2622 1293 921 862 SacII CC_GC'GG Cuts at: 2757 2757 Size: 12268 SalI G'TCGA_C Cuts at: 1936 7913 9610 1936 Size: 5977 1697 4594 Fragments arranged by size: 5977 4594 1697 SanDI GG'GwC_CC Cuts at: 2279 2879 4203 7563 2279 Size: 600 1324 3360 6984 Fragments arranged by size: 6984 3360 1324 600 SapI GCTCTTCn'nnn_ Cuts at: 2117 2216 2687 4049 5603 5768 8585 2117 Size: 99 471 1362 1554 165 2817 5800 Fragments arranged by size: 5800 2817 1554 1362 471 165 99 SbfI CC_TGCA'GG Cuts at: 6672 9620 6672 Size: 2948 9320 ScaI AGT'ACT Cuts at: 3740 4958 10516 12228 3740 Size: 1218 5558 1712 3780 Fragments arranged by size: 5558 3780 1712 1218 SexAI A'CCwGG_T Cuts at: 2699 7362 7998 2699 Size: 4663 636 6969 Fragments arranged by size: 6969 4663 636 SfcI C'TryA_G Cuts at: 826 1045 2287 2513 3859 4091 5455 5615 5894 Size: 219 1242 226 1346 232 1364 160 279 Cuts at: 5894 6590 6668 7784 9125 9227 9616 10751 11432 Size: 696 78 1116 1341 102 389 1135 681 Cuts at: 11432 11623 826 Size: 191 1471 Fragments arranged by size: 1471 1364 1346 1341 1242 1135 1116 696 681 389 279 232 226 219 191 160 102 78 SfiI GGCCn_nnn'nGGCC Cuts at: 3202 3202 Size: 12268 SfoI GGC'GCC Cuts at: 2426 2773 4847 6962 2426 Size: 347 2074 2115 7732 Fragments arranged by size: 7732 2115 2074 347 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12268 SmaI CCC'GGG Cuts at: 1872 3223 6889 9595 1872 Size: 1351 3666 2706 4545 Fragments arranged by size: 4545 3666 2706 1351 SmlI C'TyrA_G Cuts at: 820 1017 1943 2864 3881 4625 4952 6002 8645 Size: 197 926 921 1017 744 327 1050 2643 Cuts at: 8645 9029 9437 10372 11273 11535 820 Size: 384 408 935 901 262 1553 Fragments arranged by size: 2643 1553 1050 1017 935 926 921 901 744 408 384 327 262 197 SnaBI TAC'GTA Cuts at: 493 10059 493 Size: 9566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12268 SphI G_CATG'C Cuts at: 1132 9626 1132 Size: 8494 3774 SspI AAT'ATT Cuts at: 5 52 10192 5 Size: 47 10140 2081 Fragments arranged by size: 10140 2081 47 StuI AGG'CCT Cuts at: 1517 3887 8720 1517 Size: 2370 4833 5065 Fragments arranged by size: 5065 4833 2370 StyI C'CwwG_G Cuts at: 513 1065 2004 2161 2811 3459 3550 4560 5802 Size: 552 939 157 650 648 91 1010 1242 Cuts at: 5802 6144 7446 8046 9261 9707 513 Size: 342 1302 600 1215 446 3074 Fragments arranged by size: 3074 1302 1242 1215 1010 939 650 648 600 552 446 342 157 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4465 6346 8365 8519 9257 10295 10312 Size: 1255 2885 1881 2019 154 738 1038 17 Cuts at: 10312 10465 10650 11095 325 Size: 153 185 445 1498 Fragments arranged by size: 2885 2019 1881 1498 1255 1038 738 445 185 154 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2034 3738 4464 Size: 274 80 33 51 1443 57 1704 726 Cuts at: 4464 4956 5664 6708 8295 8781 9078 10514 12226 Size: 492 708 1044 1587 486 297 1436 1712 Cuts at: 12226 96 Size: 138 Fragments arranged by size: 1712 1704 1587 1443 1436 1044 726 708 492 486 297 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5444 5476 6648 6680 8363 8395 8937 8969 9404 Size: 32 1172 32 1683 32 542 32 435 Cuts at: 9404 9436 12228 12260 5444 Size: 32 2792 32 5452 Fragments arranged by size: 5452 2792 1683 1172 542 435 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5870 8798 9281 1316 Size: 4554 2928 483 4303 Fragments arranged by size: 4554 4303 2928 483 XbaI T'CTAG_A Cuts at: 9604 9604 Size: 12268 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2365 4348 5809 6204 6460 6857 2365 Size: 1983 1461 395 256 397 7776 Fragments arranged by size: 7776 1983 1461 397 395 256 XhoI C'TCGA_G Cuts at: 1943 9029 1943 Size: 7086 5182 XmaI C'CCGG_G Cuts at: 1870 3221 6887 9593 1870 Size: 1351 3666 2706 4545 Fragments arranged by size: 4545 3666 2706 1351 XmnI GAAnn'nnTTC Cuts at: 4769 10397 4769 Size: 5628 6640 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4396 7882 10076 276 Size: 53 83 186 936 2862 3486 2194 2468 Fragments arranged by size: 3486 2862 2468 2194 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NsiI PacI SrfI SwaI