Restriction map for
pFN21AA0231
(Circular) (Six-base) MAPSORT of: pFN21AA0231.seq Check: 3456 from: 1 to: 7114
pFN21AA0231 7114 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2082 4924 278
Size: 53 83 186 936 546 2842 2468
Fragments arranged by size:
2842 2468 936 546 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3435 4457 1937
Size: 1498 1022 4594
Fragments arranged by size:
4594 1498 1022
Acc65I G'GTAC_C
Cuts at: 4435 4435
Size: 7114
AclI AA'CG_TT
Cuts at: 1563 4387 5241 5614 1563
Size: 2824 854 373 3063
Fragments arranged by size:
3063 2824 854 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3050 3884 5177 6561 6829 1632
Size: 1418 834 1293 1384 268 1917
Fragments arranged by size:
1917 1418 1384 1293 834 268
AfeI AGC'GCT
Cuts at: 2906 2906
Size: 7114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6917
AflIII A'CryG_T
Cuts at: 1203 6013 6990 1203
Size: 4810 977 1327
Fragments arranged by size:
4810 1327 977
AgeI A'CCGG_T
Cuts at: 5945 5945
Size: 7114
AhdI GACnn_n'nnGTC
Cuts at: 5843 5843
Size: 7114
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4140 4199 1184
Size: 2956 59 4099
Fragments arranged by size:
4099 2956 59
AlwNI CAG_nnn'CTG
Cuts at: 2525 3700 4105 4857 6429 2525
Size: 1175 405 752 1572 3210
Fragments arranged by size:
3210 1572 1175 752 405
ApaLI G'TGCA_C
Cuts at: 5171 6327 5171
Size: 1156 5958
ApoI r'AATT_y
Cuts at: 1493 2186 2280 3466 3488 4219 4423 4649 4801
Size: 693 94 1186 22 731 204 226 152
Cuts at: 4801 6910 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1186 731 693 226 204 152
94 22
AseI AT'TA_AT
Cuts at: 160 1032 5668 160
Size: 872 4636 1606
Fragments arranged by size:
4636 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 2450 4439 1870
Size: 73 507 1989 4545
Fragments arranged by size:
4545 1989 507 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2419 2452 2419
Size: 33 7081
BamHI G'GATC_C
Cuts at: 3657 4444 4811 3657
Size: 787 367 5960
Fragments arranged by size:
5960 787 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4435 5890 618
Size: 325 217 627 2648 1455 1842
Fragments arranged by size:
2648 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2137 4269 4433 1391
Size: 746 2132 164 4072
Fragments arranged by size:
4072 2132 746 164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2074 2162 3290 928
Size: 971 175 88 1128 4752
Fragments arranged by size:
4752 1128 971 175 88
BbvCI CC'TCA_GC
Cuts at: 3017 3017
Size: 7114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5305 5339 1903
Size: 34 3368 34 3678
Fragments arranged by size:
3678 3368 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5006 6216 5006
Size: 1210 5904
BclI T'GATC_A
Cuts at: 1548 2103 3124 3780 1548
Size: 555 1021 656 4882
Fragments arranged by size:
4882 1021 656 555
BfrBI ATG'CAT
Cuts at: 2743 2743
Size: 7114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 5725 136
Size: 107 122 71 631 605 454 3599 1525
Fragments arranged by size:
3599 1525 631 605 454 122 107 71
BglII A'GATC_T
Cuts at: 7109 7109
Size: 7114
BlpI GC'TnA_GC
Cuts at: 2032 2526 3338 4531 2032
Size: 494 812 1193 4615
Fragments arranged by size:
4615 1193 812 494
Bme1580I G_kGCm'C
Cuts at: 1790 5175 6331 1790
Size: 3385 1156 2573
Fragments arranged by size:
3385 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4398 1318
Size: 3080 4034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2038 2427 4845
Size: 416 53 83 399 24 621 389 2418
Cuts at: 4845 5803 442
Size: 958 1753
Fragments arranged by size:
2418 1753 958 621 416 399 389 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4003 4003
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 3365 4283 5774 1239
Size: 884 1242 918 1491 2579
Fragments arranged by size:
2579 1491 1242 918 884
Bpu10I CC'TnA_GC
Cuts at: 3017 3017
Size: 7114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3383 3515 3524 5239 6104 6402 3383
Size: 132 9 1715 865 298 4095
Fragments arranged by size:
4095 1715 865 298 132 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5777 882
Size: 617 4278 2219
Fragments arranged by size:
4278 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4905 493
Size: 711 3701 2702
Fragments arranged by size:
3701 2702 711
BsaBI GATnn'nnATC
Cuts at: 7108 7108
Size: 7114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 4921 5303 5938
Size: 53 83 186 936 546 2842 382 635
Cuts at: 5938 275
Size: 1451
Fragments arranged by size:
2842 1451 936 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5547 5945 6219 6366 6777 5547
Size: 398 274 147 411 5884
Fragments arranged by size:
5884 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4279 4309 1178
Size: 30 165 30 2876 30 3983
Fragments arranged by size:
3983 2876 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3034 3476 4361 1186
Size: 1848 442 885 3939
Fragments arranged by size:
3939 1848 885 442
BseYI C'CCAG_C
Cuts at: 3516 6317 6813 3516
Size: 2801 496 3817
Fragments arranged by size:
3817 2801 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2903 3429 3936 1163
Size: 573 1167 526 507 4341
Fragments arranged by size:
4341 1167 573 526 507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4564 5325 6353 1540
Size: 275 186 2563 761 1028 2301
Fragments arranged by size:
2563 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2137 2382 2817 4269 5175 5260 6331 2137
Size: 245 435 1452 906 85 1071 2920
Fragments arranged by size:
2920 1452 1071 906 435 245 85
BsmI GAATG_Cn'
Cuts at: 4625 4718 4625
Size: 93 7021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2382 2817 4269 4433 5175 5260
Size: 399 347 245 435 1452 164 742 85
Cuts at: 5260 6331 1391
Size: 1071 2174
Fragments arranged by size:
2174 1452 1071 742 435 399 347 245
164 85
BspEI T'CCGG_A
Cuts at: 6777 6777
Size: 7114
BspHI T'CATG_A
Cuts at: 5003 6733 5003
Size: 1730 5384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4469 844
Size: 3625 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2269 5001 1394
Size: 429 446 2732 3507
Fragments arranged by size:
3507 2732 446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2618 3689 3936 5609 5783 65
Size: 1183 1370 1071 247 1673 174 1396
Fragments arranged by size:
1673 1396 1370 1183 1071 247 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5758 5945 1700
Size: 110 3948 187 2869
Fragments arranged by size:
3948 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7114
BssSI C'ACGA_G
Cuts at: 5174 6186 5174
Size: 1012 6102
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7114
BstBI TT'CG_AA
Cuts at: 5918 5918
Size: 7114
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7114
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 2680
Size: 7114
BstYI r'GATC_y
Cuts at: 1972 3657 4057 4444 4811 5196 5213 6665 6751
Size: 1685 400 387 367 385 17 1452 86
Cuts at: 6751 6763 7109 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1685 1452 400 387 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2581 3848 2581
Size: 1267 5847
BtgI C'CryG_G
Cuts at: 513 1065 2069 2492 513
Size: 552 1004 423 5135
Fragments arranged by size:
5135 1004 552 423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6375
Fragments arranged by size:
6375 683 56
BtsI GCAGTG_nn'
Cuts at: 4193 4630 5424 5444 4193
Size: 437 794 20 5863
Fragments arranged by size:
5863 794 437 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7079
DraI TTT'AAA
Cuts at: 3471 4418 3471
Size: 947 6167
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6121 809
Size: 507 4805 1802
Fragments arranged by size:
4805 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2127 2463
Size: 54 1425 50 275 18 288 9 336
Cuts at: 2463 4561 5450 7079 8
Size: 2098 889 1629 43
Fragments arranged by size:
2098 1629 1425 889 336 288 275 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4561 1537
Size: 275 2749 4090
Fragments arranged by size:
4090 2749 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2998 3635 3870 4371 5044 1353
Size: 1645 637 235 501 673 3423
Fragments arranged by size:
3423 1645 673 637 501 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3470 5701 6075 6221 387
Size: 1064 138 1881 2231 374 146 1280
Fragments arranged by size:
2231 1881 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2135 4267 2135
Size: 2132 4982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 3050 3365 3884 4283 5177 5774
Size: 393 491 927 315 519 399 894 597
Cuts at: 5774 6561 6829 1239
Size: 787 268 1524
Fragments arranged by size:
1524 927 894 787 597 519 491 399
393 315 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3170 3170
Size: 7114
EcoO109I rG'GnC_Cy
Cuts at: 2944 3627 2944
Size: 683 6431
EcoRI G'AATT_C
Cuts at: 2280 4423 4801 2280
Size: 2143 378 4593
Fragments arranged by size:
4593 2143 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2685 3115 2685
Size: 430 6684
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 2908 6261 1678
Size: 1230 3353 2531
Fragments arranged by size:
3353 2531 1230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 3745 3777 5755
Size: 32 135 32 458 32 1683 32 1978
Cuts at: 5755 5787 5829 5861 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1978 1683 458 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3394 4458 4704 669
Size: 988 281 1383 73 1064 246 3079
Fragments arranged by size:
3079 1383 1064 988 281 246 73
HindIII A'AGCT_T
Cuts at: 748 3242 748
Size: 2494 4620
HpaI GTT'AAC
Cuts at: 3321 4704 3321
Size: 1383 5731
KpnI G_GTAC'C
Cuts at: 4439 4439
Size: 7114
MfeI C'AATT_G
Cuts at: 4713 4713
Size: 7114
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3576 6228 6412 1305
Size: 2271 2652 184 2007
Fragments arranged by size:
2652 2271 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 10
Size: 54 1425 343 633 4659
Fragments arranged by size:
4659 1425 633 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 4236 5072 5431 5590 518
Size: 648 1578 1492 836 359 159 2042
Fragments arranged by size:
2042 1578 1492 836 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2594 2651 3388 3786 4530 5207 6355
Size: 537 451 57 737 398 744 677 1148
Cuts at: 6355 6600 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 744 737 677 537 451 398
245 57
NcoI C'CATG_G
Cuts at: 513 1065 2069 2492 513
Size: 552 1004 423 5135
Fragments arranged by size:
5135 1004 552 423
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7114
NotI GC'GGCC_GC
Cuts at: 4561 4561
Size: 7114
NsiI A_TGCA'T
Cuts at: 2745 2745
Size: 7114
NspI r_CATG'y
Cuts at: 1132 1229 2743 2747 4472 6017 1132
Size: 97 1514 4 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 1514 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7114
PciI A'CATG_T
Cuts at: 6013 6013
Size: 7114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7114
PfoI T'CCnGG_A
Cuts at: 2679 3653 2679
Size: 974 6140
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2228 2260 5183 5215 6696 6728 2228
Size: 32 2923 32 1481 32 2614
Fragments arranged by size:
2923 2614 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2944 3627 2944
Size: 683 6431
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7114
PsiI TTA'TAA
Cuts at: 4684 4684
Size: 7114
PspXI vC'TCGA_Gb
Cuts at: 2450 2450
Size: 7114
PstI C_TGCA'G
Cuts at: 830 2411 3379 4188 4466 830
Size: 1581 968 809 278 3478
Fragments arranged by size:
3478 1581 968 809 278
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7114
PvuII CAG'CTG
Cuts at: 2143 2594 2651 3388 3786 2143
Size: 451 57 737 398 5471
Fragments arranged by size:
5471 737 451 398 57
SacI G_AGCT'C
Cuts at: 2137 4269 2137
Size: 2132 4982
SalI G'TCGA_C
Cuts at: 1936 4456 1936
Size: 2520 4594
SapI GCTCTTCn'nnn_
Cuts at: 3635 3635
Size: 7114
SbfI CC_TGCA'GG
Cuts at: 4466 4466
Size: 7114
ScaI AGT'ACT
Cuts at: 2309 3586 4121 5362 7074 2309
Size: 1277 535 1241 1712 2349
Fragments arranged by size:
2349 1712 1277 1241 535
SfcI C'TryA_G
Cuts at: 826 1045 2407 2998 3070 3375 4184 4462 5597
Size: 219 1362 591 72 305 809 278 1135
Cuts at: 5597 6278 6469 826
Size: 681 191 1471
Fragments arranged by size:
1471 1362 1135 809 681 591 305 278
219 191 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 2126 2126
Size: 7114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7114
SmaI CCC'GGG
Cuts at: 1872 4441 1872
Size: 2569 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2450 3398 3530 3539 5218 6119
Size: 197 926 507 948 132 9 1679 901
Cuts at: 6119 6381 820
Size: 262 1553
Fragments arranged by size:
1679 1553 948 926 901 507 262 197
132 9
SnaBI TAC'GTA
Cuts at: 493 4905 493
Size: 4412 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 2743 4472 1132
Size: 1611 1729 3774
Fragments arranged by size:
3774 1729 1611
SspI AAT'ATT
Cuts at: 5 52 3038 4006 4144 5038 5
Size: 47 2986 968 138 894 2081
Fragments arranged by size:
2986 2081 968 894 138 47
StuI AGG'CCT
Cuts at: 1517 2542 1517
Size: 1025 6089
StyI C'CwwG_G
Cuts at: 513 1065 2069 2210 2492 2925 4553 513
Size: 552 1004 141 282 433 1628 3074
Fragments arranged by size:
3074 1628 1004 552 433 282 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4226 5141 5158 5311 5496 5941 325
Size: 1255 2646 915 17 153 185 445 1498
Fragments arranged by size:
2646 1498 1255 915 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 3584 3633
Size: 274 80 33 51 1443 330 1277 49
Cuts at: 3633 4119 5360 7072 96
Size: 486 1241 1712 138
Fragments arranged by size:
1712 1443 1277 1241 486 330 274 138
80 51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7074 7106 7074
Size: 32 7082
Tth111I GACn'n_nGTC
Cuts at: 1316 3308 1316
Size: 1992 5122
XbaI T'CTAG_A
Cuts at: 3457 4450 3457
Size: 993 6121
XhoI C'TCGA_G
Cuts at: 1943 2450 1943
Size: 507 6607
XmaI C'CCGG_G
Cuts at: 1870 4439 1870
Size: 2569 4545
XmnI GAAnn'nnTTC
Cuts at: 2732 4290 5243 2732
Size: 1558 953 4603
Fragments arranged by size:
4603 1558 953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2080 4922 276
Size: 53 83 186 936 546 2842 2468
Fragments arranged by size:
2842 2468 936 546 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacII
SanDI SexAI SfoI SrfI SwaI XcmI