Restriction map for
pFN21AA0219
(Circular) (Six-base) MAPSORT of: pFN21AA0219.seq Check: 5696 from: 1 to: 12718
pFN21AA0219 12718 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2300 8580 9985 2300
Size: 6280 1405 5033
Fragments arranged by size:
6280 5033 1405
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10528 278
Size: 53 83 186 936 8992 2468
Fragments arranged by size:
8992 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4647 6610 10061 1937
Size: 2710 1963 3451 4594
Fragments arranged by size:
4594 3451 2710 1963
Acc65I G'GTAC_C
Cuts at: 10039 10039
Size: 12718
AclI AA'CG_TT
Cuts at: 1563 10845 11218 1563
Size: 9282 373 3063
Fragments arranged by size:
9282 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2863 3170 3591 3848 4119 4454 4688 4808
Size: 1231 307 421 257 271 335 234 120
Cuts at: 4808 4979 5009 5166 5933 6263 8219 8384 9047
Size: 171 30 157 767 330 1956 165 663
Cuts at: 9047 9119 9399 9806 9980 10781 12165 12433 1632
Size: 72 280 407 174 801 1384 268 1917
Fragments arranged by size:
1956 1917 1384 1231 801 767 663 421
407 335 330 307 280 271 268 257
234 174 171 165 157 120 72 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12521
AflIII A'CryG_T
Cuts at: 1203 6927 7814 9423 11617 12594 1203
Size: 5724 887 1609 2194 977 1327
Fragments arranged by size:
5724 2194 1609 1327 977 887
AgeI A'CCGG_T
Cuts at: 11549 11549
Size: 12718
AhdI GACnn_n'nnGTC
Cuts at: 11447 11447
Size: 12718
AleI CACnn'nnGTG
Cuts at: 1166 2986 4760 4989 8279 1166
Size: 1820 1774 229 3290 5605
Fragments arranged by size:
5605 3290 1820 1774 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2228 3004 3048 4278 4326 8532 1184
Size: 1044 776 44 1230 48 4206 5370
Fragments arranged by size:
5370 4206 1230 1044 776 48 44
AlwNI CAG_nnn'CTG
Cuts at: 2446 3148 3391 3509 3543 4555 4913 5176 5351
Size: 702 243 118 34 1012 358 263 175
Cuts at: 5351 6157 6160 6799 7151 7574 8125 8306 9226
Size: 806 3 639 352 423 551 181 920
Cuts at: 9226 10461 12033 2446
Size: 1235 1572 3131
Fragments arranged by size:
3131 1572 1235 1012 920 806 702 639
551 423 358 352 263 243 181 175
118 34 3
ApaI G_GGCC'C
Cuts at: 7335 7335
Size: 12718
ApaLI G'TGCA_C
Cuts at: 10775 11931 10775
Size: 1156 11562
ApoI r'AATT_y
Cuts at: 1493 2774 3590 10027 10253 10405 12514 1493
Size: 1281 816 6437 226 152 2109 1697
Fragments arranged by size:
6437 2109 1697 1281 816 226 152
AseI AT'TA_AT
Cuts at: 160 1032 11272 160
Size: 872 10240 1606
Fragments arranged by size:
10240 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12718
AvaI C'yCGr_G
Cuts at: 1870 1943 2534 5075 7889 8277 8587 8984 10043
Size: 73 591 2541 2814 388 310 397 1059
Cuts at: 10043 1870
Size: 4545
Fragments arranged by size:
4545 2814 2541 1059 591 397 388 310
73
AvrII C'CTAG_G
Cuts at: 9352 9352
Size: 12718
BamHI G'GATC_C
Cuts at: 6763 9567 10048 10415 6763
Size: 2804 481 367 9066
Fragments arranged by size:
9066 2804 481 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4116 4818 8448 9348 10039
Size: 325 217 627 2329 702 3630 900 691
Cuts at: 10039 11494 618
Size: 1455 1842
Fragments arranged by size:
3630 2329 1842 1455 900 702 691 627
325 217
BanII G_rGCy'C
Cuts at: 1391 2154 2580 3709 3972 4190 5159 5978 6319
Size: 763 426 1129 263 218 969 819 341
Cuts at: 6319 7133 7335 7791 10037 1391
Size: 814 202 456 2246 4072
Fragments arranged by size:
4072 2246 1129 969 819 814 763 456
426 341 263 218 202
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 3836 4939 5123 6782 928
Size: 971 381 1556 1103 184 1659 6864
Fragments arranged by size:
6864 1659 1556 1103 971 381 184
BbvCI CC'TCA_GC
Cuts at: 2238 3522 5051 7167 9340 2238
Size: 1284 1529 2116 2173 5616
Fragments arranged by size:
5616 2173 2116 1529 1284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3455 3489 3749 3783 10909 10943 1903
Size: 34 1518 34 260 34 7126 34 3678
Fragments arranged by size:
7126 3678 1518 260 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7358 10610 11820 7358
Size: 3252 1210 8256
Fragments arranged by size:
8256 3252 1210
BclI T'GATC_A
Cuts at: 1548 4017 1548
Size: 2469 10249
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2216 2348
Size: 107 122 71 631 605 337 207 132
Cuts at: 2348 4738 6210 7049 9977 11329 136
Size: 2390 1472 839 2928 1352 1525
Fragments arranged by size:
2928 2390 1525 1472 1352 839 631 605
337 207 132 122 107 71
BglII A'GATC_T
Cuts at: 2125 7855 12713 2125
Size: 5730 4858 2130
Fragments arranged by size:
5730 4858 2130
BlpI GC'TnA_GC
Cuts at: 3165 3756 9299 10135 3165
Size: 591 5543 836 5748
Fragments arranged by size:
5748 5543 836 591
Bme1580I G_kGCm'C
Cuts at: 1790 2746 3270 4351 4757 6627 7335 8028 8250
Size: 956 524 1081 406 1870 708 693 222
Cuts at: 8250 8451 10779 11935 1790
Size: 201 2328 1156 2573
Fragments arranged by size:
2573 2328 1870 1156 1081 956 708 693
524 406 222 201
BmgBI CAC'GTC
Cuts at: 1318 2647 3742 5260 5506 9424 1318
Size: 1329 1095 1518 246 3918 4612
Fragments arranged by size:
4612 3918 1518 1329 1095 246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2313 4621 5639
Size: 416 53 83 399 24 896 2308 1018
Cuts at: 5639 5734 7563 7600 10449 11407 442
Size: 95 1829 37 2849 958 1753
Fragments arranged by size:
2849 2308 1829 1753 1018 958 896 416
399 95 83 53 37 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12718
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 7147 2108
Size: 5039 7679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 3906 4448 4589 5670 6147 6182 6542
Size: 1238 1429 542 141 1081 477 35 360
Cuts at: 6542 6723 6754 8078 8147 8417 8495 8599 8651
Size: 181 31 1324 69 270 78 104 52
Cuts at: 8651 11378 1239
Size: 2727 2579
Fragments arranged by size:
2727 2579 1429 1324 1238 1081 542 477
360 270 181 141 104 78 69 52
35 31
Bpu10I CC'TnA_GC
Cuts at: 2238 3522 3725 5051 6591 6689 7144 7167 8835
Size: 1284 203 1326 1540 98 455 23 1668
Cuts at: 8835 9009 9340 9545 2238
Size: 174 331 205 5411
Fragments arranged by size:
5411 1668 1540 1326 1284 455 331 205
203 174 98 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3956 4727 6493 7928 9848 10843 11708 12006 3956
Size: 771 1766 1435 1920 995 865 298 4668
Fragments arranged by size:
4668 1920 1766 1435 995 865 771 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7929 8590 11381 882
Size: 617 6430 661 2791 2219
Fragments arranged by size:
6430 2791 2219 661 617
BsaAI yAC'GTr
Cuts at: 493 1204 3121 3916 4771 6382 7815 10509 493
Size: 711 1917 795 855 1611 1433 2694 2702
Fragments arranged by size:
2702 2694 1917 1611 1433 855 795 711
BsaBI GATnn'nnATC
Cuts at: 4088 8459 8939 12712 4088
Size: 4371 480 3773 4094
Fragments arranged by size:
4371 4094 3773 480
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6980 7712 8895 10525
Size: 53 83 186 936 5447 732 1183 1630
Cuts at: 10525 10907 11542 275
Size: 382 635 1451
Fragments arranged by size:
5447 1630 1451 1183 936 732 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3956 4536 5427 5820 8451 9273 11151 11549 11823
Size: 580 891 393 2631 822 1878 398 274
Cuts at: 11823 11970 12381 3956
Size: 147 411 4293
Fragments arranged by size:
4293 2631 1878 891 822 580 411 398
393 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3097 3127 3929 3959 4154
Size: 30 165 30 1694 30 802 30 195
Cuts at: 4154 4184 4991 5021 5471 5501 5720 5750 6518
Size: 30 807 30 450 30 219 30 768
Cuts at: 6518 6548 6917 6947 7845 7875 1178
Size: 30 369 30 898 30 6021
Fragments arranged by size:
6021 1694 898 807 802 768 450 369
219 195 165 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2833 3836 4301 4454 4484 5009 5012 5015
Size: 1647 1003 465 153 30 525 3 3
Cuts at: 5015 5018 5264 5432 5435 5673 5918 6539 8075
Size: 3 246 168 3 238 245 621 1536
Cuts at: 8075 8183 8648 8861 9328 9398 9851 1186
Size: 108 465 213 467 70 453 4053
Fragments arranged by size:
4053 1647 1536 1003 621 525 467 465
465 453 246 245 238 213 168 153
108 70 30 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2244 2464 2602 4313 4350 4512 5046 6060 6349
Size: 220 138 1711 37 162 534 1014 289
Cuts at: 6349 6548 6971 7458 7692 7795 8344 8640 9042
Size: 199 423 487 234 103 549 296 402
Cuts at: 9042 9307 11921 12417 2244
Size: 265 2614 496 2545
Fragments arranged by size:
2614 2545 1711 1014 549 534 496 487
423 402 296 289 265 234 220 199
162 138 103 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 2608 2636 3010 4070 4248 4274
Size: 573 723 149 28 374 1060 178 26
Cuts at: 4274 4496 4850 6325 7137 7839 8693 1163
Size: 222 354 1475 812 702 854 5188
Fragments arranged by size:
5188 1475 1060 854 812 723 702 573
374 354 222 178 149 28 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5639 10168 10929 11957 1540
Size: 275 186 3638 4529 761 1028 2301
Fragments arranged by size:
4529 3638 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3723 5313 6481 6501 6589 7133 10779 10864 11935
Size: 1590 1168 20 88 544 3646 85 1071
Cuts at: 11935 3723
Size: 4506
Fragments arranged by size:
4506 3646 1590 1168 1071 544 88 85
20
BsmI GAATG_Cn'
Cuts at: 4183 8101 10229 10322 4183
Size: 3918 2128 93 6579
Fragments arranged by size:
6579 3918 2128 93
BsmBI CGTCTCn'nnnn_
Cuts at: 9292 9292
Size: 12718
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2580 2746 3270 3709 3723 3972
Size: 399 364 426 166 524 439 14 249
Cuts at: 3972 4190 4351 4757 5159 5313 5978 6319 6481
Size: 218 161 406 402 154 665 341 162
Cuts at: 6481 6501 6589 6627 7133 7335 7791 8028 8250
Size: 20 88 38 506 202 456 237 222
Cuts at: 8250 8451 10037 10779 10864 11935 1391
Size: 201 1586 742 85 1071 2174
Fragments arranged by size:
2174 1586 1071 742 665 524 506 456
439 426 406 402 399 364 341 249
237 222 218 202 201 166 162 161
154 88 85 38 20 14
BspEI T'CCGG_A
Cuts at: 4536 5427 5820 9273 12381 4536
Size: 891 393 3453 3108 4873
Fragments arranged by size:
4873 3453 3108 891 393
BspHI T'CATG_A
Cuts at: 3199 5515 7974 9609 10607 12337 3199
Size: 2316 2459 1635 998 1730 3580
Fragments arranged by size:
3580 2459 2316 1730 1635 998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2011 2300 2372 3351 3562 4522 5478 6055
Size: 1167 289 72 979 211 960 956 577
Cuts at: 6055 6975 7299 8580 9985 10073 844
Size: 920 324 1281 1405 88 3489
Fragments arranged by size:
3489 1405 1281 1167 979 960 956 920
577 324 289 211 88 72
BsrBI CCG'CTC
Cuts at: 1394 1823 7645 7683 8547 9585 10605 1394
Size: 429 5822 38 864 1038 1020 3507
Fragments arranged by size:
5822 3507 1038 1020 864 429 38
BsrDI GCAATG_nn'
Cuts at: 65 1248 6579 6732 7245 9573 11213 11387 65
Size: 1183 5331 153 513 2328 1640 174 1396
Fragments arranged by size:
5331 2328 1640 1396 1183 513 174 153
BsrFI r'CCGG_y
Cuts at: 1700 1810 5634 6303 7178 9509 9646 11362 11549
Size: 110 3824 669 875 2331 137 1716 187
Cuts at: 11549 1700
Size: 2869
Fragments arranged by size:
3824 2869 2331 1716 875 669 187 137
110
BsrGI T'GTAC_A
Cuts at: 96 7671 96
Size: 7575 5143
BssHII G'CGCG_C
Cuts at: 1925 7399 1925
Size: 5474 7244
BssSI C'ACGA_G
Cuts at: 2626 6101 8021 10778 11790 2626
Size: 3475 1920 2757 1012 3554
Fragments arranged by size:
3554 3475 2757 1920 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2446 4076 6157 6799 9412 794
Size: 1652 1630 2081 642 2613 4100
Fragments arranged by size:
4100 2613 2081 1652 1630 642
BstBI TT'CG_AA
Cuts at: 11522 11522
Size: 12718
BstEII G'GTnAC_C
Cuts at: 1184 2918 1184
Size: 1734 10984
BstXI CCAn_nnnn'nTGG
Cuts at: 4120 4891 5694 5992 6674 6920 7240 8623 9910
Size: 771 803 298 682 246 320 1383 1287
Cuts at: 9910 4120
Size: 6928
Fragments arranged by size:
6928 1383 1287 803 771 682 320 298
246
BstYI r'GATC_y
Cuts at: 1972 2125 2782 5028 5424 6723 6763 7525 7767
Size: 153 657 2246 396 1299 40 762 242
Cuts at: 7767 7855 8467 9567 9906 10048 10415 10800 10817
Size: 88 612 1100 339 142 367 385 17
Cuts at: 10817 12269 12355 12367 12713 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2246 1977 1452 1299 1100 762 657 612
396 385 367 346 339 242 153 142
88 86 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 7017 7919 8493 8863 8900 9227 7017
Size: 902 574 370 37 327 10508
Fragments arranged by size:
10508 902 574 370 327 37
BtgI C'CryG_G
Cuts at: 513 1065 2004 2487 2892 3006 3923 4182 6888
Size: 552 939 483 405 114 917 259 2706
Cuts at: 6888 7053 7097 8028 8910 9186 9474 9555 9822
Size: 165 44 931 882 276 288 81 267
Cuts at: 9822 9987 513
Size: 165 3244
Fragments arranged by size:
3244 2706 939 931 917 882 552 483
405 288 276 267 259 165 165 114
81 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6022 6981 7426 7950 487
Size: 683 56 4796 959 445 524 5255
Fragments arranged by size:
5255 4796 959 683 524 445 56
BtsI GCAGTG_nn'
Cuts at: 2148 2256 2335 2447 2966 2998 3353 4242 4265
Size: 108 79 112 519 32 355 889 23
Cuts at: 4265 5221 5568 7626 9413 9529 9556 9746 10234
Size: 956 347 2058 1787 116 27 190 488
Cuts at: 10234 11028 11048 2148
Size: 794 20 3818
Fragments arranged by size:
3818 2058 1787 956 889 794 519 488
355 347 190 116 112 108 79 32
27 23 20
ClaI AT'CG_AT
Cuts at: 1554 2182 4092 1554
Size: 628 1910 10180
Fragments arranged by size:
10180 1910 628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7765 7800 529
Size: 35 7201 35 5447
Fragments arranged by size:
7201 5447 35 35
DraI TTT'AAA
Cuts at: 10022 10022
Size: 12718
DraIII CAC_nnn'GTG
Cuts at: 2488 3124 3853 5202 6487 2488
Size: 636 729 1349 1285 8719
Fragments arranged by size:
8719 1349 1285 729 636
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2916 3138 6656 9200 11725 809
Size: 507 1600 222 3518 2544 2525 1802
Fragments arranged by size:
3518 2544 2525 1802 1600 507 222
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3003 4064 4602
Size: 54 1425 50 275 18 1173 1061 538
Cuts at: 4602 5118 5636 5982 6729 7176 7806 8113 8403
Size: 516 518 346 747 447 630 307 290
Cuts at: 8403 9648 9918 9969 10165 11054 12683 8
Size: 1245 270 51 196 889 1629 43
Fragments arranged by size:
1629 1425 1245 1173 1061 889 747 630
538 518 516 447 346 307 290 275
270 196 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5636 10165 1537
Size: 275 3824 4529 4090
Fragments arranged by size:
4529 4090 3824 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3004 4821 4841 7925 8609 8697 9161 9375
Size: 1651 1817 20 3084 684 88 464 214
Cuts at: 9375 9798 9876 10648 1353
Size: 423 78 772 3423
Fragments arranged by size:
3423 3084 1817 1651 772 684 464 423
214 88 78 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 5603 6290 6553 7663 8588
Size: 1064 138 438 3576 687 263 1110 925
Cuts at: 8588 8867 9434 9472 9575 9648 11305 11679 11825
Size: 279 567 38 103 73 1657 374 146
Cuts at: 11825 387
Size: 1280
Fragments arranged by size:
3576 1657 1280 1110 1064 925 687 567
438 374 279 263 146 138 103 73
38
EcoICRI GAG'CTC
Cuts at: 7131 7131
Size: 12718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 2863 3170 3591 3848 3906 4119
Size: 393 845 386 307 421 257 58 213
Cuts at: 4119 4448 4454 4589 4688 4808 4979 5009 5166
Size: 329 6 135 99 120 171 30 157
Cuts at: 5166 5670 5933 6147 6182 6263 6542 6723 6754
Size: 504 263 214 35 81 279 181 31
Cuts at: 6754 8078 8147 8219 8384 8417 8495 8599 8651
Size: 1324 69 72 165 33 78 104 52
Cuts at: 8651 9047 9119 9399 9806 9980 10781 11378 12165
Size: 396 72 280 407 174 801 597 787
Cuts at: 12165 12433 1239
Size: 268 1524
Fragments arranged by size:
1524 1324 845 801 787 597 504 421
407 396 393 386 329 307 280 279
268 263 257 214 213 181 174 171
165 157 135 120 104 99 81 78
72 72 69 58 52 35 33 31
30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2203 2815 3188 3935 6218 6524 2203
Size: 612 373 747 2283 306 8397
Fragments arranged by size:
8397 2283 747 612 373 306
EcoO109I rG'GnC_Cy
Cuts at: 2208 3145 3207 3388 3928 4216 5055 6223 7042
Size: 937 62 181 540 288 839 1168 819
Cuts at: 7042 7331 8379 8904 9193 9223 9742 9831 9954
Size: 289 1048 525 289 30 519 89 123
Cuts at: 9954 2208
Size: 4972
Fragments arranged by size:
4972 1168 1048 937 839 819 540 525
519 289 289 288 181 123 89 62
30
EcoRI G'AATT_C
Cuts at: 10027 10405 10027
Size: 378 12340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4720 5788 6313 7016 8058 9404 4720
Size: 1068 525 703 1042 1346 8034
Fragments arranged by size:
8034 1346 1068 1042 703 525
FspI TGC'GCA
Cuts at: 2378 11224 2378
Size: 8846 3872
FspAI rTGC'GCAy
Cuts at: 2378 2378
Size: 12718
HaeII r_GCGC'y
Cuts at: 1678 2670 4580 6689 7152 11865 1678
Size: 992 1910 2109 463 4713 2531
Fragments arranged by size:
4713 2531 2109 1910 992 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2183 2196 2215 3097
Size: 32 135 32 592 19 13 19 882
Cuts at: 3097 3129 5471 5503 5962 5994 6120 6152 6515
Size: 32 2342 32 459 32 126 32 363
Cuts at: 6515 6547 6821 6853 8159 8191 8754 8786 9606
Size: 32 274 32 1306 32 563 32 820
Cuts at: 9606 9638 11359 11391 11433 11465 1373
Size: 32 1721 32 42 32 2626
Fragments arranged by size:
2626 2342 1721 1306 882 820 592 563
459 363 274 135 126 42 32 32
32 32 32 32 32 32 32 32
32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4648 5881 8272 9856 9946 10062
Size: 988 281 2710 1233 2391 1584 90 116
Cuts at: 10062 10308 669
Size: 246 3079
Fragments arranged by size:
3079 2710 2391 1584 1233 988 281 246
116 90
HindIII A'AGCT_T
Cuts at: 748 3297 4395 6629 8372 748
Size: 2549 1098 2234 1743 5094
Fragments arranged by size:
5094 2549 2234 1743 1098
HpaI GTT'AAC
Cuts at: 9946 10308 9946
Size: 362 12356
KpnI G_GTAC'C
Cuts at: 10043 10043
Size: 12718
MfeI C'AATT_G
Cuts at: 8407 9849 10317 8407
Size: 1442 468 10808
Fragments arranged by size:
10808 1442 468
MluI A'CGCG_T
Cuts at: 12594 12594
Size: 12718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2712 2955 5814 7179 7635 9105 11832 12016
Size: 1407 243 2859 1365 456 1470 2727 184
Cuts at: 12016 1305
Size: 2007
Fragments arranged by size:
2859 2727 2007 1470 1407 1365 456 243
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4066 4604 5120 5984 6731
Size: 54 1425 343 2234 538 516 864 747
Cuts at: 6731 7808 8405 9920 9971 10
Size: 1077 597 1515 51 2757
Fragments arranged by size:
2757 2234 1515 1425 1077 864 747 597
538 516 343 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 2986 4760 4989 5814 7277 8279
Size: 648 1533 287 1774 229 825 1463 1002
Cuts at: 8279 9143 9578 10676 11035 11194 518
Size: 864 435 1098 359 159 2042
Fragments arranged by size:
2042 1774 1533 1463 1098 1002 864 825
648 435 359 287 229 159
MspA1I CmG'CkG
Cuts at: 1606 4480 5050 5578 6087 6157 6598 7411 7696
Size: 2874 570 528 509 70 441 813 285
Cuts at: 7696 8098 8158 8348 8395 8961 9046 10134 10811
Size: 402 60 190 47 566 85 1088 677
Cuts at: 10811 11959 12204 1606
Size: 1148 245 2120
Fragments arranged by size:
2874 2120 1148 1088 813 677 570 566
528 509 441 402 285 245 190 85
70 60 47
NaeI GCC'GGC
Cuts at: 6305 9511 9648 6305
Size: 3206 137 9375
Fragments arranged by size:
9375 3206 137
NcoI C'CATG_G
Cuts at: 513 1065 2004 2892 3006 4182 7053 9186 513
Size: 552 939 888 114 1176 2871 2133 4045
Fragments arranged by size:
4045 2871 2133 1176 939 888 552 114
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12718
NgoMIV G'CCGG_C
Cuts at: 6303 9509 9646 6303
Size: 3206 137 9375
Fragments arranged by size:
9375 3206 137
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12718
NotI GC'GGCC_GC
Cuts at: 10165 10165
Size: 12718
NspI r_CATG'y
Cuts at: 1132 1229 2602 3338 3355 5833 6931 7724 8756
Size: 97 1373 736 17 2478 1098 793 1032
Cuts at: 8756 9307 10076 11621 1132
Size: 551 769 1545 2229
Fragments arranged by size:
2478 2229 1545 1373 1098 1032 793 769
736 551 97 17
PasI CC'CwG_GG
Cuts at: 1348 7633 1348
Size: 6285 6433
PciI A'CATG_T
Cuts at: 6927 11617 6927
Size: 4690 8028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2233 3421 5563 5746 7699 8125 1108
Size: 1125 1188 2142 183 1953 426 5701
Fragments arranged by size:
5701 2142 1953 1188 1125 426 183
PfoI T'CCnGG_A
Cuts at: 4803 6069 6747 6770 6876 9909 4803
Size: 1266 678 23 106 3033 7612
Fragments arranged by size:
7612 3033 1266 678 106 23
PmeI GTTT'AAAC
Cuts at: 10022 10022
Size: 12718
PmlI CAC'GTG
Cuts at: 3121 4771 6382 7815 3121
Size: 1650 1611 1433 8024
Fragments arranged by size:
8024 1650 1611 1433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5867 5899 10787 10819 12300 12332 5867
Size: 32 4888 32 1481 32 6253
Fragments arranged by size:
6253 4888 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3145 3207 3928 4216 8379 9193 9742 9954 3145
Size: 62 721 288 4163 814 549 212 5909
Fragments arranged by size:
5909 4163 814 721 549 288 212 62
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12718
PsiI TTA'TAA
Cuts at: 10288 10288
Size: 12718
PspOMI G'GGCC_C
Cuts at: 7331 7331
Size: 12718
PstI C_TGCA'G
Cuts at: 830 2286 3348 3936 4509 4551 5475 6442 9051
Size: 1456 1062 588 573 42 924 967 2609
Cuts at: 9051 9075 9222 9684 10070 830
Size: 24 147 462 386 3478
Fragments arranged by size:
3478 2609 1456 1062 967 924 588 573
462 386 147 42 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12718
PvuII CAG'CTG
Cuts at: 4480 5050 6087 6157 6598 7696 8098 8158 8395
Size: 570 1037 70 441 1098 402 60 237
Cuts at: 8395 8961 9046 4480
Size: 566 85 8152
Fragments arranged by size:
8152 1098 1037 570 566 441 402 237
85 70 60
RsrII CG'GwC_CG
Cuts at: 4210 7026 4210
Size: 2816 9902
SacI G_AGCT'C
Cuts at: 7133 7133
Size: 12718
SalI G'TCGA_C
Cuts at: 1936 4646 10060 1936
Size: 2710 5414 4594
Fragments arranged by size:
5414 4594 2710
SanDI GG'GwC_CC
Cuts at: 8379 8379
Size: 12718
SapI GCTCTTCn'nnn_
Cuts at: 4821 7925 8697 9798 4821
Size: 3104 772 1101 7741
Fragments arranged by size:
7741 3104 1101 772
SbfI CC_TGCA'GG
Cuts at: 3936 9075 10070 3936
Size: 5139 995 6584
Fragments arranged by size:
6584 5139 995
ScaI AGT'ACT
Cuts at: 2552 10966 12678 2552
Size: 8414 1712 2592
Fragments arranged by size:
8414 2592 1712
SfcI C'TryA_G
Cuts at: 826 1045 2282 3344 3932 4505 4547 5471 6438
Size: 219 1237 1062 588 573 42 924 967
Cuts at: 6438 7227 9047 9071 9218 9401 9680 9835 10066
Size: 789 1820 24 147 183 279 155 231
Cuts at: 10066 11201 11882 12073 826
Size: 1135 681 191 1471
Fragments arranged by size:
1820 1471 1237 1135 1062 967 924 789
681 588 573 279 231 219 191 183
155 147 42 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 6210 7049 9977 6210
Size: 839 2928 8951
Fragments arranged by size:
8951 2928 839
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12718
SgrAI Cr'CCGG_yG
Cuts at: 1700 6303 1700
Size: 4603 8115
SmaI CCC'GGG
Cuts at: 1872 2536 5077 8279 10045 1872
Size: 664 2541 3202 1766 4545
Fragments arranged by size:
4545 3202 2541 1766 664
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3971 4742 6472 7907 8984 9863
Size: 197 926 2028 771 1730 1435 1077 879
Cuts at: 9863 10822 11723 11985 820
Size: 959 901 262 1553
Fragments arranged by size:
2028 1730 1553 1435 1077 959 926 901
879 771 262 197
SnaBI TAC'GTA
Cuts at: 493 10509 493
Size: 10016 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12718
SphI G_CATG'C
Cuts at: 1132 2602 3338 9307 10076 1132
Size: 1470 736 5969 769 3774
Fragments arranged by size:
5969 3774 1470 769 736
SspI AAT'ATT
Cuts at: 5 52 10642 5
Size: 47 10590 2081
Fragments arranged by size:
10590 2081 47
StuI AGG'CCT
Cuts at: 1517 3911 7473 7917 1517
Size: 2394 3562 444 6318
Fragments arranged by size:
6318 3562 2394 444
StyI C'CwwG_G
Cuts at: 513 1065 2004 2892 3006 3210 4182 4713 6453
Size: 552 939 888 114 204 972 531 1740
Cuts at: 6453 7045 7053 7519 8187 9186 9352 9807 10157
Size: 592 8 466 668 999 166 455 350
Cuts at: 10157 513
Size: 3074
Fragments arranged by size:
3074 1740 999 972 939 888 668 592
552 531 466 455 350 204 166 114
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 4047 4886 5131 5386 5820 5826
Size: 1255 876 1591 839 245 255 434 6
Cuts at: 5826 6662 7706 10745 10762 10915 11100 11545 325
Size: 836 1044 3039 17 153 185 445 1498
Fragments arranged by size:
3039 1591 1498 1255 1044 876 839 836
445 434 255 245 185 153 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2550 3623 4629
Size: 274 80 33 51 1443 573 1073 1006
Cuts at: 4629 6930 7671 10964 12676 96
Size: 2301 741 3293 1712 138
Fragments arranged by size:
3293 2301 1712 1443 1073 1006 741 573
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2426 2458 2912 2944 3436 3468 3605 3637 3929
Size: 32 454 32 492 32 137 32 292
Cuts at: 3929 3961 4019 4051 4138 4170 4991 5023 5690
Size: 32 58 32 87 32 821 32 667
Cuts at: 5690 5722 6518 6550 7242 7274 7845 7877 12678
Size: 32 796 32 692 32 571 32 4801
Cuts at: 12678 12710 2426
Size: 32 2434
Fragments arranged by size:
4801 2434 821 796 692 667 571 492
454 292 137 87 58 32 32 32
32 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2648 4214 1316
Size: 1332 1566 9820
Fragments arranged by size:
9820 1566 1332
XbaI T'CTAG_A
Cuts at: 10054 10054
Size: 12718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2591 4180 5710 7262 9994 2230
Size: 361 1589 1530 1552 2732 4954
Fragments arranged by size:
4954 2732 1589 1552 1530 361
XhoI C'TCGA_G
Cuts at: 1943 8984 1943
Size: 7041 5677
XmaI C'CCGG_G
Cuts at: 1870 2534 5075 8277 10043 1870
Size: 664 2541 3202 1766 4545
Fragments arranged by size:
4545 3202 2541 1766 664
XmnI GAAnn'nnTTC
Cuts at: 4703 6296 9032 10847 4703
Size: 1593 2736 1815 6574
Fragments arranged by size:
6574 2736 1815 1593
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10526 276
Size: 53 83 186 936 8992 2468
Fragments arranged by size:
8992 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FseI KasI NarI NruI NsiI PacI PspXI
PsrI SacII SexAI SfoI SrfI SwaI