Restriction map for
pFN21AA0196
(Circular) (Six-base) MAPSORT of: pFN21AA0196.seq Check: 7015 from: 1 to: 8182
pFN21AA0196 8182 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5992 278
Size: 53 83 186 936 4456 2468
Fragments arranged by size:
4456 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2325 2686 4471 5525 1937
Size: 388 361 1785 1054 4594
Fragments arranged by size:
4594 1785 1054 388 361
Acc65I G'GTAC_C
Cuts at: 5503 5503
Size: 8182
AclI AA'CG_TT
Cuts at: 1563 4460 6309 6682 1563
Size: 2897 1849 373 3063
Fragments arranged by size:
3063 2897 1849 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 4077 4325 5276 5454 6245 7629 7897
Size: 2288 157 248 951 178 791 1384 268
Cuts at: 7897 1632
Size: 1917
Fragments arranged by size:
2288 1917 1384 951 791 268 248 178
157
AfeI AGC'GCT
Cuts at: 5108 5108
Size: 8182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7985
AflIII A'CryG_T
Cuts at: 1203 4514 5440 7081 8058 1203
Size: 3311 926 1641 977 1327
Fragments arranged by size:
3311 1641 1327 977 926
AgeI A'CCGG_T
Cuts at: 7013 7013
Size: 8182
AhdI GACnn_n'nnGTC
Cuts at: 6911 6911
Size: 8182
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8182
AlwNI CAG_nnn'CTG
Cuts at: 3244 4120 4873 5290 5925 7497 3244
Size: 876 753 417 635 1572 3929
Fragments arranged by size:
3929 1572 876 753 635 417
ApaLI G'TGCA_C
Cuts at: 6239 7395 6239
Size: 1156 7026
ApoI r'AATT_y
Cuts at: 1493 2252 3533 4672 4777 4788 5355 5491 5717
Size: 759 1281 1139 105 11 567 136 226
Cuts at: 5717 5869 7978 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1281 1139 759 567 226 152
136 105 11
AseI AT'TA_AT
Cuts at: 160 1032 3804 6736 160
Size: 872 2772 2932 1606
Fragments arranged by size:
2932 2772 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8182
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 4194 4838 5507 1870
Size: 73 684 1567 644 669 4545
Fragments arranged by size:
4545 1567 684 669 644 73
BamHI G'GATC_C
Cuts at: 3228 4105 5512 5879 3228
Size: 877 1407 367 5531
Fragments arranged by size:
5531 1407 877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2603 5375 5503 6958 618
Size: 325 217 627 816 2772 128 1455 1842
Fragments arranged by size:
2772 1842 1455 816 627 325 217 128
BanII G_rGCy'C
Cuts at: 1391 5501 1391
Size: 4110 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7211
BbvCI CC'TCA_GC
Cuts at: 3986 3986
Size: 8182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6373 6407 1903
Size: 34 4436 34 3678
Fragments arranged by size:
4436 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3244 3874 6074 7284 2736
Size: 508 630 2200 1210 3634
Fragments arranged by size:
3634 2200 1210 630 508
BclI T'GATC_A
Cuts at: 1548 2143 3606 3639 1548
Size: 595 1463 33 6091
Fragments arranged by size:
6091 1463 595 33
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6793 136
Size: 107 122 71 631 605 5121 1525
Fragments arranged by size:
5121 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2693 3438 8177 2693
Size: 745 4739 2698
Fragments arranged by size:
4739 2698 745
BlpI GC'TnA_GC
Cuts at: 2223 3830 3999 5599 2223
Size: 1607 169 1600 4806
Fragments arranged by size:
4806 1607 1600 169
Bme1580I G_kGCm'C
Cuts at: 1790 5380 6243 7399 1790
Size: 3590 863 1156 2573
Fragments arranged by size:
3590 2573 1156 863
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8182
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5913 6871 442
Size: 416 53 83 399 24 4496 958 1753
Fragments arranged by size:
4496 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3873 4964 5075 5411 6842 1239
Size: 2634 1091 111 336 1431 2579
Fragments arranged by size:
2634 2579 1431 1091 336 111
Bpu10I CC'TnA_GC
Cuts at: 3986 3986
Size: 8182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3006 6307 7172 7470 3006
Size: 3301 865 298 3718
Fragments arranged by size:
3718 3301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2722 6845 882
Size: 617 1223 4123 2219
Fragments arranged by size:
4123 2219 1223 617
BsaAI yAC'GTr
Cuts at: 493 1204 2782 5973 493
Size: 711 1578 3191 2702
Fragments arranged by size:
3191 2702 1578 711
BsaBI GATnn'nnATC
Cuts at: 8176 8176
Size: 8182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5989 6371 7006 275
Size: 53 83 186 936 4456 382 635 1451
Fragments arranged by size:
4456 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6615 7013 7287 7434 7845 6615
Size: 398 274 147 411 6952
Fragments arranged by size:
6952 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3846 3876 4343 4373 1178
Size: 30 165 30 2443 30 467 30 4987
Fragments arranged by size:
4987 2443 467 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3074 3644 1186
Size: 1888 570 5724
Fragments arranged by size:
5724 1888 570
BseYI C'CCAG_C
Cuts at: 7385 7881 7385
Size: 496 7686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3047 3240 1163
Size: 573 1311 193 6105
Fragments arranged by size:
6105 1311 573 193
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5208 5632 6393 7421 1540
Size: 275 186 3207 424 761 1028 2301
Fragments arranged by size:
3207 2301 1028 761 424 275 186
BsiHKAI G_wGCw'C
Cuts at: 6243 6328 7399 6243
Size: 85 1071 7026
Fragments arranged by size:
7026 1071 85
BsmI GAATG_Cn'
Cuts at: 4750 5067 5693 5786 4750
Size: 317 626 93 7146
Fragments arranged by size:
7146 626 317 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5380 5501 6243 6328 7399 1391
Size: 399 3590 121 742 85 1071 2174
Fragments arranged by size:
3590 2174 1071 742 399 121 85
BspEI T'CCGG_A
Cuts at: 7845 7845
Size: 8182
BspHI T'CATG_A
Cuts at: 4740 6071 7801 4740
Size: 1331 1730 5121
Fragments arranged by size:
5121 1730 1331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3834 5537 844
Size: 2990 1703 3489
Fragments arranged by size:
3489 2990 1703
BsrBI CCG'CTC
Cuts at: 1394 1823 3349 6069 1394
Size: 429 1526 2720 3507
Fragments arranged by size:
3507 2720 1526 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2079 3118 4803 6677 6851 65
Size: 1183 831 1039 1685 1874 174 1396
Fragments arranged by size:
1874 1685 1396 1183 1039 831 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6826 7013 1700
Size: 110 5016 187 2869
Fragments arranged by size:
5016 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2308 3499 5333 96
Size: 2212 1191 1834 2945
Fragments arranged by size:
2945 2212 1834 1191
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8182
BssSI C'ACGA_G
Cuts at: 6242 7254 6242
Size: 1012 7170
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2939 794
Size: 2145 6037
BstBI TT'CG_AA
Cuts at: 2575 6986 2575
Size: 4411 3771
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8182
BstXI CCAn_nnnn'nTGG
Cuts at: 2202 2865 2202
Size: 663 7519
BstYI r'GATC_y
Cuts at: 1972 2693 3228 3438 4105 5512 5879 6264 6281
Size: 721 535 210 667 1407 367 385 17
Cuts at: 6281 7733 7819 7831 8177 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1407 721 667 535 385 367
346 210 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2051 2051
Size: 8182
BtgI C'CryG_G
Cuts at: 513 1065 3735 4245 5319 513
Size: 552 2670 510 1074 3376
Fragments arranged by size:
3376 2670 1074 552 510
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2726 487
Size: 683 56 1500 5943
Fragments arranged by size:
5943 1500 683 56
BtsI GCAGTG_nn'
Cuts at: 5336 5698 6492 6512 5336
Size: 362 794 20 7006
Fragments arranged by size:
7006 794 362 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8182
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2017 2052 529
Size: 35 1453 35 6659
Fragments arranged by size:
6659 1453 35 35
DraI TTT'AAA
Cuts at: 2946 3462 4731 5486 2946
Size: 516 1269 755 5642
Fragments arranged by size:
5642 1269 755 516
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7189 809
Size: 507 5873 1802
Fragments arranged by size:
5873 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 5222 5302
Size: 54 1425 50 275 18 208 3184 80
Cuts at: 5302 5629 6518 8147 8
Size: 327 889 1629 43
Fragments arranged by size:
3184 1629 1425 889 327 275 208 80
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5629 1537
Size: 275 3817 4090
Fragments arranged by size:
4090 3817 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3239 3516 3921 4589 6112 1353
Size: 1886 277 405 668 1523 3423
Fragments arranged by size:
3423 1886 1523 668 405 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4789 6769 7143 7289 387
Size: 1064 138 3200 1980 374 146 1280
Fragments arranged by size:
3200 1980 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3873 3920 4077 4325 4964 5075 5276
Size: 393 2241 47 157 248 639 111 201
Cuts at: 5276 5411 5454 6245 6842 7629 7897 1239
Size: 135 43 791 597 787 268 1524
Fragments arranged by size:
2241 1524 791 787 639 597 393 268
248 201 157 135 111 47 43
EcoO109I rG'GnC_Cy
Cuts at: 3661 4993 3661
Size: 1332 6850
EcoRI G'AATT_C
Cuts at: 3533 5491 5869 3533
Size: 1958 378 5846
Fragments arranged by size:
5846 1958 378
EcoRV GAT'ATC
Cuts at: 2164 2339 2164
Size: 175 8007
FspI TGC'GCA
Cuts at: 6688 6688
Size: 8182
HaeII r_GCGC'y
Cuts at: 1678 5110 5297 7329 1678
Size: 3432 187 2032 2531
Fragments arranged by size:
3432 2531 2032 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2637 2669 3848 3880 4819
Size: 32 135 32 1065 32 1179 32 939
Cuts at: 4819 4851 6823 6855 6897 6929 1373
Size: 32 1972 32 42 32 2626
Fragments arranged by size:
2626 1972 1179 1065 939 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2687 4291 4472 5526 5772 669
Size: 988 281 749 1604 181 1054 246 3079
Fragments arranged by size:
3079 1604 1054 988 749 281 246 181
HindIII A'AGCT_T
Cuts at: 748 3086 3423 3513 3767 3932 4028 748
Size: 2338 337 90 254 165 96 4902
Fragments arranged by size:
4902 2338 337 254 165 96 90
HpaI GTT'AAC
Cuts at: 5772 5772
Size: 8182
KpnI G_GTAC'C
Cuts at: 5507 5507
Size: 8182
MfeI C'AATT_G
Cuts at: 5124 5781 5124
Size: 657 7525
MluI A'CGCG_T
Cuts at: 8058 8058
Size: 8182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2643 3228 3525 7296 7480 1305
Size: 684 654 585 297 3771 184 2007
Fragments arranged by size:
3771 2007 684 654 585 297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2040 5224 5304 10
Size: 54 1425 343 208 3184 80 2888
Fragments arranged by size:
3184 2888 1425 343 208 80 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2672 4067 4100 6140 6499 6658 518
Size: 648 1506 1395 33 2040 359 159 2042
Fragments arranged by size:
2042 2040 1506 1395 648 359 159 33
MspA1I CmG'CkG
Cuts at: 1606 3241 4531 4937 5063 5598 6275 7423 7668
Size: 1635 1290 406 126 535 677 1148 245
Cuts at: 7668 1606
Size: 2120
Fragments arranged by size:
2120 1635 1290 1148 677 535 406 245
126
NcoI C'CATG_G
Cuts at: 513 1065 3735 4245 513
Size: 552 2670 510 4450
Fragments arranged by size:
4450 2670 552 510
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8182
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8182
NotI GC'GGCC_GC
Cuts at: 5629 5629
Size: 8182
NspI r_CATG'y
Cuts at: 1132 1229 3020 3907 4405 4518 5444 5540 7085
Size: 97 1791 887 498 113 926 96 1545
Cuts at: 7085 1132
Size: 2229
Fragments arranged by size:
2229 1791 1545 926 887 498 113 97
96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8182
PciI A'CATG_T
Cuts at: 4514 5440 7081 4514
Size: 926 1641 5615
Fragments arranged by size:
5615 1641 926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3126 3544 1108
Size: 2018 418 5746
Fragments arranged by size:
5746 2018 418
PfoI T'CCnGG_A
Cuts at: 5388 5388
Size: 8182
PmeI GTTT'AAAC
Cuts at: 5486 5486
Size: 8182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6251 6283 7764 7796 6251
Size: 32 1481 32 6637
Fragments arranged by size:
6637 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3661 4993 3661
Size: 1332 6850
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8182
PsiI TTA'TAA
Cuts at: 5752 5752
Size: 8182
PspXI vC'TCGA_Gb
Cuts at: 4194 4194
Size: 8182
PstI C_TGCA'G
Cuts at: 830 4481 5365 5534 830
Size: 3651 884 169 3478
Fragments arranged by size:
3651 3478 884 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8182
PvuII CAG'CTG
Cuts at: 3241 4531 4937 5063 3241
Size: 1290 406 126 6360
Fragments arranged by size:
6360 1290 406 126
SalI G'TCGA_C
Cuts at: 1936 2685 4470 5524 1936
Size: 749 1785 1054 4594
Fragments arranged by size:
4594 1785 1054 749
SbfI CC_TGCA'GG
Cuts at: 5534 5534
Size: 8182
ScaI AGT'ACT
Cuts at: 3845 6430 8142 3845
Size: 2585 1712 3885
Fragments arranged by size:
3885 2585 1712
SexAI A'CCwGG_T
Cuts at: 2599 2712 2599
Size: 113 8069
SfcI C'TryA_G
Cuts at: 826 1045 2234 4477 5361 5530 6665 7346 7537
Size: 219 1189 2243 884 169 1135 681 191
Cuts at: 7537 826
Size: 1471
Fragments arranged by size:
2243 1471 1189 1135 884 681 219 191
169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8182
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8182
SmaI CCC'GGG
Cuts at: 1872 5509 1872
Size: 3637 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3021 4194 4838 6286 7187 7449
Size: 197 926 1078 1173 644 1448 901 262
Cuts at: 7449 820
Size: 1553
Fragments arranged by size:
1553 1448 1173 1078 926 901 644 262
197
SnaBI TAC'GTA
Cuts at: 493 5973 493
Size: 5480 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8182
SphI G_CATG'C
Cuts at: 1132 3020 5540 1132
Size: 1888 2520 3774
Fragments arranged by size:
3774 2520 1888
SspI AAT'ATT
Cuts at: 5 52 3453 6106 5
Size: 47 3401 2653 2081
Fragments arranged by size:
3401 2653 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8182
StyI C'CwwG_G
Cuts at: 513 1065 3735 4245 4605 5621 513
Size: 552 2670 510 360 1016 3074
Fragments arranged by size:
3074 2670 1016 552 510 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2831 5222 5363 6209 6226 6379 6564
Size: 1255 1251 2391 141 846 17 153 185
Cuts at: 6564 7009 325
Size: 445 1498
Fragments arranged by size:
2391 1498 1255 1251 846 445 185 153
141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2308 2433 2579
Size: 274 80 33 51 1443 331 125 146
Cuts at: 2579 3499 3843 5333 6428 8140 96
Size: 920 344 1490 1095 1712 138
Fragments arranged by size:
1712 1490 1443 1095 920 344 331 274
146 138 125 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4343 4375 4405 4437 5217 5249 8142 8174 4343
Size: 32 30 32 780 32 2893 32 4351
Fragments arranged by size:
4351 2893 780 32 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 1316 4286 1316
Size: 2970 5212
XbaI T'CTAG_A
Cuts at: 2341 5518 2341
Size: 3177 5005
XhoI C'TCGA_G
Cuts at: 1943 4194 4838 1943
Size: 2251 644 5287
Fragments arranged by size:
5287 2251 644
XmaI C'CCGG_G
Cuts at: 1870 5507 1870
Size: 3637 4545
XmnI GAAnn'nnTTC
Cuts at: 2253 3417 3525 5261 6311 2253
Size: 1164 108 1736 1050 4124
Fragments arranged by size:
4124 1736 1164 1050 108
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5990 276
Size: 53 83 186 936 4456 2468
Fragments arranged by size:
4456 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI