Restriction map for
pFN21AA0185
(Circular) (Six-base) MAPSORT of: pFN21AA0185.seq Check: 1629 from: 1 to: 10318
pFN21AA0185 10318 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2371 4049 4906 6900 2371
Size: 1678 857 1994 5789
Fragments arranged by size:
5789 1994 1678 857
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7446 8128 278
Size: 53 83 186 936 5910 682 2468
Fragments arranged by size:
5910 2468 936 682 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7661 1937
Size: 5724 4594
Acc65I G'GTAC_C
Cuts at: 2579 3442 7639 2579
Size: 863 4197 5258
Fragments arranged by size:
5258 4197 863
AclI AA'CG_TT
Cuts at: 1563 8445 8818 1563
Size: 6882 373 3063
Fragments arranged by size:
6882 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 2423 2576 3182 3561 5270 5594 6141
Size: 504 287 153 606 379 1709 324 547
Cuts at: 6141 6591 6940 7178 8381 9765 10033 1632
Size: 450 349 238 1203 1384 268 1917
Fragments arranged by size:
1917 1709 1384 1203 606 547 504 450
379 349 324 287 268 238 153
AfeI AGC'GCT
Cuts at: 7514 7514
Size: 10318
AflII C'TTAA_G
Cuts at: 820 1017 6941 820
Size: 197 5924 4197
Fragments arranged by size:
5924 4197 197
AflIII A'CryG_T
Cuts at: 1203 6507 6981 7011 9217 10194 1203
Size: 5304 474 30 2206 977 1327
Fragments arranged by size:
5304 2206 1327 977 474 30
AgeI A'CCGG_T
Cuts at: 9149 9149
Size: 10318
AhdI GACnn_n'nnGTC
Cuts at: 2852 4928 9047 2852
Size: 2076 4119 4123
Fragments arranged by size:
4123 4119 2076
AleI CACnn'nnGTG
Cuts at: 1166 2921 5793 1166
Size: 1755 2872 5691
Fragments arranged by size:
5691 2872 1755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10318
AlwNI CAG_nnn'CTG
Cuts at: 2719 3706 4459 4522 5129 5611 5983 6275 8061
Size: 987 753 63 607 482 372 292 1786
Cuts at: 8061 9633 2719
Size: 1572 3404
Fragments arranged by size:
3404 1786 1572 987 753 607 482 372
292 63
ApaI G_GGCC'C
Cuts at: 6940 6940
Size: 10318
ApaLI G'TGCA_C
Cuts at: 3026 4721 8375 9531 3026
Size: 1695 3654 1156 3813
Fragments arranged by size:
3813 3654 1695 1156
ApoI r'AATT_y
Cuts at: 1493 4295 5535 7121 7627 7853 8005 10114 1493
Size: 2802 1240 1586 506 226 152 2109 1697
Fragments arranged by size:
2802 2109 1697 1586 1240 506 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8872 160
Size: 872 7840 1606
Fragments arranged by size:
7840 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10318
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 5274 7643 1870
Size: 73 88 3243 2369 4545
Fragments arranged by size:
4545 3243 2369 88 73
BamHI G'GATC_C
Cuts at: 2123 2944 4159 5062 6793 7648 8015 2123
Size: 821 1215 903 1731 855 367 4426
Fragments arranged by size:
4426 1731 1215 903 855 821 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 3442 3709 5228 7199
Size: 325 217 627 792 863 267 1519 1971
Cuts at: 7199 7639 9094 618
Size: 440 1455 1842
Fragments arranged by size:
1971 1842 1519 1455 863 792 627 440
325 267 217
BanII G_rGCy'C
Cuts at: 1391 3726 6674 6848 6940 7258 7604 7637 1391
Size: 2335 2948 174 92 318 346 33 4072
Fragments arranged by size:
4072 2948 2335 346 318 174 92 33
BbeI G_GCGC'C
Cuts at: 7203 7203
Size: 10318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2604 3211 3947 5267 5306 5829 6597
Size: 971 705 607 736 1320 39 523 768
Cuts at: 6597 928
Size: 4649
Fragments arranged by size:
4649 1320 971 768 736 705 607 523
39
BbvCI CC'TCA_GC
Cuts at: 2547 4769 6276 2547
Size: 2222 1507 6589
Fragments arranged by size:
6589 2222 1507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3066 3100 6535 6569 8509 8543 1903
Size: 34 1129 34 3435 34 1940 34 3678
Fragments arranged by size:
3678 3435 1940 1129 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2596 5201 8210 9420 2596
Size: 2605 3009 1210 3494
Fragments arranged by size:
3494 3009 2605 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2325 5615 8929
Size: 107 122 71 631 605 653 3290 3314
Cuts at: 8929 136
Size: 1525
Fragments arranged by size:
3314 3290 1525 653 631 605 122 107
71
BglII A'GATC_T
Cuts at: 10313 10313
Size: 10318
BlpI GC'TnA_GC
Cuts at: 3191 6842 7367 7735 3191
Size: 3651 525 368 5774
Fragments arranged by size:
5774 3651 525 368
Bme1580I G_kGCm'C
Cuts at: 1790 3030 3380 3714 4614 4725 5231 6940 8379
Size: 1240 350 334 900 111 506 1709 1439
Cuts at: 8379 9535 1790
Size: 1156 2573
Fragments arranged by size:
2573 1709 1439 1240 1156 900 506 350
334 111
BmgBI CAC'GTC
Cuts at: 1318 6217 6508 1318
Size: 4899 291 5128
Fragments arranged by size:
5128 4899 291
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6213 8049 9007
Size: 416 53 83 399 24 4796 1836 958
Cuts at: 9007 442
Size: 1753
Fragments arranged by size:
4796 1836 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10318
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5248 5968 5248
Size: 720 9598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 3977 4037 4481 5399 7025 7280 7601
Size: 1067 1671 60 444 918 1626 255 321
Cuts at: 7601 8978 1239
Size: 1377 2579
Fragments arranged by size:
2579 1671 1626 1377 1067 918 444 321
255 60
Bpu10I CC'TnA_GC
Cuts at: 2547 2862 3032 4769 6276 2547
Size: 315 170 1737 1507 6589
Fragments arranged by size:
6589 1737 1507 315 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4928 5522 5705 7334 8443 9308 9606 4928
Size: 594 183 1629 1109 865 298 5640
Fragments arranged by size:
5640 1629 1109 865 594 298 183
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4141 6279 8981 882
Size: 617 2642 2138 2702 2219
Fragments arranged by size:
2702 2642 2219 2138 617
BsaAI yAC'GTr
Cuts at: 493 1204 8109 493
Size: 711 6905 2702
Fragments arranged by size:
6905 2702 711
BsaBI GATnn'nnATC
Cuts at: 10312 10312
Size: 10318
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3064 5813 7200 7443
Size: 53 83 186 936 1531 2749 1387 243
Cuts at: 7443 8125 8507 9142 275
Size: 682 382 635 1451
Fragments arranged by size:
2749 1531 1451 1387 936 682 635 382
243 186 83 53
BsaWI w'CCGG_w
Cuts at: 3481 7149 8751 9149 9423 9570 9981 3481
Size: 3668 1602 398 274 147 411 3818
Fragments arranged by size:
3818 3668 1602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2745 2775 3476 3506 5087
Size: 30 165 30 1342 30 701 30 1581
Cuts at: 5087 5117 5789 5819 6998 7028 1178
Size: 30 672 30 1179 30 4468
Fragments arranged by size:
4468 1581 1342 1179 701 672 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2049 2769 4382 4430 4433 4478 6272 6293
Size: 863 720 1613 48 3 45 1794 21
Cuts at: 6293 6362 6500 6545 6698 6794 6977 7231 1186
Size: 69 138 45 153 96 183 254 4273
Fragments arranged by size:
4273 1794 1613 863 720 254 183 153
138 96 69 48 45 45 21 3
BseYI C'CCAG_C
Cuts at: 3143 6075 6362 7308 7324 9521 10017 3143
Size: 2932 287 946 16 2197 496 3444
Fragments arranged by size:
3444 2932 2197 946 496 287 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3719 4583 4820 7079 7577 1163
Size: 573 1983 864 237 2259 498 3904
Fragments arranged by size:
3904 2259 1983 864 573 498 237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7768 8529 9557 1540
Size: 275 186 5767 761 1028 2301
Fragments arranged by size:
5767 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3030 3726 4725 6674 6848 7373 7604 8379 8464
Size: 696 999 1949 174 525 231 775 85
Cuts at: 8464 9535 3030
Size: 1071 3813
Fragments arranged by size:
3813 1949 1071 999 775 696 525 231
174 85
BsmI GAATG_Cn'
Cuts at: 2259 2448 4197 7829 7922 2259
Size: 189 1749 3632 93 4655
Fragments arranged by size:
4655 3632 1749 189 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4835 5752 5804 5912 6536 4835
Size: 917 52 108 624 8617
Fragments arranged by size:
8617 917 624 108 52
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3030 3380 3714 3726 4614 4725 5231
Size: 399 1240 350 334 12 888 111 506
Cuts at: 5231 6674 6848 6940 7258 7373 7604 7637 8379
Size: 1443 174 92 318 115 231 33 742
Cuts at: 8379 8464 9535 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1443 1240 1071 888 742 506 399
350 334 318 231 174 115 111 92
85 33 12
BspEI T'CCGG_A
Cuts at: 3481 9981 3481
Size: 6500 3818
BspHI T'CATG_A
Cuts at: 3346 4284 8207 9937 3346
Size: 938 3923 1730 3727
Fragments arranged by size:
3923 3727 1730 938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2371 3614 4049 4906 6900 7673 844
Size: 1527 1243 435 857 1994 773 3489
Fragments arranged by size:
3489 1994 1527 1243 857 773 435
BsrBI CCG'CTC
Cuts at: 1394 1823 7339 7462 8205 1394
Size: 429 5516 123 743 3507
Fragments arranged by size:
5516 3507 743 429 123
BsrDI GCAATG_nn'
Cuts at: 65 1248 2799 4824 7293 8813 8987 65
Size: 1183 1551 2025 2469 1520 174 1396
Fragments arranged by size:
2469 2025 1551 1520 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4993 5447 6473 8962 9149 1700
Size: 110 3183 454 1026 2489 187 2869
Fragments arranged by size:
3183 2869 2489 1026 454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10318
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10318
BssSI C'ACGA_G
Cuts at: 8378 9390 8378
Size: 1012 9306
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3000 4381 4477 794
Size: 2206 1381 96 6635
Fragments arranged by size:
6635 2206 1381 96
BstBI TT'CG_AA
Cuts at: 5341 9122 5341
Size: 3781 6537
BstEII G'GTnAC_C
Cuts at: 1184 4351 1184
Size: 3167 7151
BstXI CCAn_nnnn'nTGG
Cuts at: 3095 3457 7133 7579 3095
Size: 362 3676 446 5834
Fragments arranged by size:
5834 3676 446 362
BstYI r'GATC_y
Cuts at: 1972 2049 2123 2944 4159 5062 5938 6793 7648
Size: 77 74 821 1215 903 876 855 855
Cuts at: 7648 8015 8400 8417 9869 9955 9967 10313 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1215 903 876 855 855 821
385 367 346 86 77 74 17 12
Bsu36I CC'TnA_GG
Cuts at: 3228 4252 5612 3228
Size: 1024 1360 7934
Fragments arranged by size:
7934 1360 1024
BtgI C'CryG_G
Cuts at: 513 1065 2004 2602 5421 5703 6816 6900 6924
Size: 552 939 598 2819 282 1113 84 24
Cuts at: 6924 513
Size: 3907
Fragments arranged by size:
3907 2819 1113 939 598 552 282 84
24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4628 487
Size: 683 56 3402 6177
Fragments arranged by size:
6177 3402 683 56
BtsI GCAGTG_nn'
Cuts at: 2651 2988 3108 3372 4391 5268 5391 5865 7834
Size: 337 120 264 1019 877 123 474 1969
Cuts at: 7834 8628 8648 2651
Size: 794 20 4321
Fragments arranged by size:
4321 1969 1019 877 794 474 337 264
123 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5608 5643 529
Size: 35 5044 35 5204
Fragments arranged by size:
5204 5044 35 35
DraI TTT'AAA
Cuts at: 7622 7622
Size: 10318
DraIII CAC_nnn'GTG
Cuts at: 6017 6019 7243 6017
Size: 2 1224 9092
Fragments arranged by size:
9092 1224 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 9325 809
Size: 507 3818 4191 1802
Fragments arranged by size:
4191 3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2796 3769 4153
Size: 54 1425 50 275 18 966 973 384
Cuts at: 4153 4524 5366 5386 5449 5602 5700 7765 8654
Size: 371 842 20 63 153 98 2065 889
Cuts at: 8654 10283 8
Size: 1629 43
Fragments arranged by size:
2065 1629 1425 973 966 889 842 384
371 275 153 98 63 54 50 43
20 18
EagI C'GGCC_G
Cuts at: 1537 1812 7765 1537
Size: 275 5953 4090
Fragments arranged by size:
5953 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2489 4208 6534 8248 1353
Size: 759 377 1719 2326 1714 3423
Fragments arranged by size:
3423 2326 1719 1714 759 377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8905 9279 9425 387
Size: 1064 138 7316 374 146 1280
Fragments arranged by size:
7316 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3724 6672 6846 7602 3724
Size: 2948 174 756 6440
Fragments arranged by size:
6440 2948 756 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2306 2423 2576 3182 3561 3977
Size: 393 504 170 117 153 606 379 416
Cuts at: 3977 4037 4481 5270 5399 5594 6141 6591 6940
Size: 60 444 789 129 195 547 450 349
Cuts at: 6940 7025 7178 7280 7601 8381 8978 9765 10033
Size: 85 153 102 321 780 597 787 268
Cuts at: 10033 1239
Size: 1524
Fragments arranged by size:
1524 789 787 780 606 597 547 504
450 444 416 393 379 349 321 268
195 170 153 153 129 117 102 85
60
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 4736 6482 7037 2246
Size: 2490 1746 555 5527
Fragments arranged by size:
5527 2490 1746 555
EcoO109I rG'GnC_Cy
Cuts at: 2407 2551 2988 3436 5608 5672 6145 6936 6937
Size: 144 437 448 2172 64 473 791 1
Cuts at: 6937 7576 2407
Size: 639 5149
Fragments arranged by size:
5149 2172 791 639 473 448 437 144
64 1
EcoRI G'AATT_C
Cuts at: 5535 7627 8005 5535
Size: 2092 378 7848
Fragments arranged by size:
7848 2092 378
EcoRV GAT'ATC
Cuts at: 3344 3344
Size: 10318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3235 4147 5668 5996 6260 7522 3235
Size: 912 1521 328 264 1262 6031
Fragments arranged by size:
6031 1521 1262 912 328 264
FseI GG_CCGG'CC
Cuts at: 5451 5451
Size: 10318
FspI TGC'GCA
Cuts at: 4481 8824 4481
Size: 4343 5975
HaeII r_GCGC'y
Cuts at: 1678 6590 6788 7203 7516 9465 1678
Size: 4912 198 415 313 1949 2531
Fragments arranged by size:
4912 2531 1949 415 313 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2838 2870 3054 3086 3463
Size: 32 135 32 1266 32 184 32 377
Cuts at: 3463 3495 4649 4681 4914 4946 5789 5821 5841
Size: 32 1154 32 233 32 843 32 20
Cuts at: 5841 5873 7382 7414 8959 8991 9033 9065 1373
Size: 32 1509 32 1545 32 42 32 2626
Fragments arranged by size:
2626 1545 1509 1266 1154 843 377 233
184 135 42 32 32 32 32 32
32 32 32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 2929 7662 7908 669
Size: 988 281 592 399 4733 246 3079
Fragments arranged by size:
4733 3079 988 592 399 281 246
HindIII A'AGCT_T
Cuts at: 748 2023 2067 5681 748
Size: 1275 44 3614 5385
Fragments arranged by size:
5385 3614 1275 44
HpaI GTT'AAC
Cuts at: 7908 7908
Size: 10318
KasI G'GCGC_C
Cuts at: 7199 7199
Size: 10318
KpnI G_GTAC'C
Cuts at: 2583 3446 7643 2583
Size: 863 4197 5258
Fragments arranged by size:
5258 4197 863
MfeI C'AATT_G
Cuts at: 7917 7917
Size: 10318
MluI A'CGCG_T
Cuts at: 10194 10194
Size: 10318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3993 4383 4554 4695 6720 9432 9616 1305
Size: 2688 390 171 141 2025 2712 184 2007
Fragments arranged by size:
2712 2688 2025 2007 390 184 171 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3771 4155 5388 5604 5702
Size: 54 1425 343 1939 384 1233 216 98
Cuts at: 5702 10
Size: 4626
Fragments arranged by size:
4626 1939 1425 1233 384 343 216 98
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3470 5793 7287 8276 8635 8794
Size: 648 1755 549 2323 1494 989 359 159
Cuts at: 8794 518
Size: 2042
Fragments arranged by size:
2323 2042 1755 1494 989 648 549 359
159
MspA1I CmG'CkG
Cuts at: 1606 4378 6601 6818 7324 7734 8411 9559 9804
Size: 2772 2223 217 506 410 677 1148 245
Cuts at: 9804 1606
Size: 2120
Fragments arranged by size:
2772 2223 2120 1148 677 506 410 245
217
NaeI GCC'GGC
Cuts at: 4995 5449 6475 4995
Size: 454 1026 8838
Fragments arranged by size:
8838 1026 454
NarI GG'CG_CC
Cuts at: 7200 7200
Size: 10318
NcoI C'CATG_G
Cuts at: 513 1065 2004 2602 5421 5703 513
Size: 552 939 598 2819 282 5128
Fragments arranged by size:
5128 2819 939 598 552 282
NdeI CA'TA_TG
Cuts at: 387 3400 387
Size: 3013 7305
NgoMIV G'CCGG_C
Cuts at: 4993 5447 6473 4993
Size: 454 1026 8838
Fragments arranged by size:
8838 1026 454
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10318
NotI GC'GGCC_GC
Cuts at: 7765 7765
Size: 10318
NspI r_CATG'y
Cuts at: 1132 1229 2569 7015 7286 7676 9221 1132
Size: 97 1340 4446 271 390 1545 2229
Fragments arranged by size:
4446 2229 1545 1340 390 271 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10318
PciI A'CATG_T
Cuts at: 7011 9217 7011
Size: 2206 8112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4522 4744 6867 1108
Size: 3414 222 2123 4559
Fragments arranged by size:
4559 3414 2123 222
PfoI T'CCnGG_A
Cuts at: 4186 5349 5958 7125 7446 4186
Size: 1163 609 1167 321 7058
Fragments arranged by size:
7058 1167 1163 609 321
PmeI GTTT'AAAC
Cuts at: 7622 7622
Size: 10318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8387 8419 9900 9932 8387
Size: 32 1481 32 8773
Fragments arranged by size:
8773 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2407 5672 6145 2407
Size: 3265 473 6580
Fragments arranged by size:
6580 3265 473
PshAI GACnn'nnGTC
Cuts at: 1613 4410 7040 1613
Size: 2797 2630 4891
Fragments arranged by size:
4891 2797 2630
PsiI TTA'TAA
Cuts at: 6105 7888 6105
Size: 1783 8535
PspOMI G'GGCC_C
Cuts at: 6936 6936
Size: 10318
PstI C_TGCA'G
Cuts at: 830 3636 3874 4473 5872 6600 6876 6897 7231
Size: 2806 238 599 1399 728 276 21 334
Cuts at: 7231 7251 7670 830
Size: 20 419 3478
Fragments arranged by size:
3478 2806 1399 728 599 419 334 276
238 21 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10318
PvuII CAG'CTG
Cuts at: 6601 7324 6601
Size: 723 9595
SacI G_AGCT'C
Cuts at: 3726 6674 6848 7604 3726
Size: 2948 174 756 6440
Fragments arranged by size:
6440 2948 756 174
SacII CC_GC'GG
Cuts at: 6819 6819
Size: 10318
SalI G'TCGA_C
Cuts at: 1936 7660 1936
Size: 5724 4594
SapI GCTCTTCn'nnn_
Cuts at: 4208 4208
Size: 10318
SbfI CC_TGCA'GG
Cuts at: 3874 6897 7670 3874
Size: 3023 773 6522
Fragments arranged by size:
6522 3023 773
ScaI AGT'ACT
Cuts at: 3730 8566 10278 3730
Size: 4836 1712 3770
Fragments arranged by size:
4836 3770 1712
SexAI A'CCwGG_T
Cuts at: 4617 6171 4617
Size: 1554 8764
SfcI C'TryA_G
Cuts at: 826 1045 3053 3274 3632 3870 4469 5076 5868
Size: 219 2008 221 358 238 599 607 792
Cuts at: 5868 6596 6872 6893 7227 7247 7666 8801 9482
Size: 728 276 21 334 20 419 1135 681
Cuts at: 9482 9673 826
Size: 191 1471
Fragments arranged by size:
2008 1471 1135 792 728 681 607 599
419 358 334 276 238 221 219 191
21 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 5615 5615
Size: 10318
SfoI GGC'GCC
Cuts at: 7201 7201
Size: 10318
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10318
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10318
SmaI CCC'GGG
Cuts at: 1872 7645 1872
Size: 5773 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4943 5501 5684 6941 7313 8422
Size: 197 926 3000 558 183 1257 372 1109
Cuts at: 8422 9323 9585 820
Size: 901 262 1553
Fragments arranged by size:
3000 1553 1257 1109 926 901 558 372
262 197 183
SnaBI TAC'GTA
Cuts at: 493 8109 493
Size: 7616 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10318
SphI G_CATG'C
Cuts at: 1132 2569 7676 1132
Size: 1437 5107 3774
Fragments arranged by size:
5107 3774 1437
SspI AAT'ATT
Cuts at: 5 52 8242 5
Size: 47 8190 2081
Fragments arranged by size:
8190 2081 47
StuI AGG'CCT
Cuts at: 1517 3233 1517
Size: 1716 8602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2602 3094 4131 4833 5421 5703
Size: 552 939 598 492 1037 702 588 282
Cuts at: 5703 6031 6231 6558 7038 7572 7757 513
Size: 328 200 327 480 534 185 3074
Fragments arranged by size:
3074 1037 939 702 598 588 552 534
492 480 328 327 282 200 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6846 6976 7390 8345 8362 8515 8700
Size: 1255 5266 130 414 955 17 153 185
Cuts at: 8700 9145 325
Size: 445 1498
Fragments arranged by size:
5266 1498 1255 955 445 414 185 153
130 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 3728 6510
Size: 274 80 33 51 1443 693 1058 2782
Cuts at: 6510 6876 7062 8564 10276 96
Size: 366 186 1502 1712 138
Fragments arranged by size:
2782 1712 1502 1443 1058 693 366 274
186 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5087 5119 6071 6103 10278 10310 5087
Size: 32 952 32 4175 32 5095
Fragments arranged by size:
5095 4175 952 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3879 4619 5117 5828 5887 1316
Size: 2563 740 498 711 59 5747
Fragments arranged by size:
5747 2563 740 711 498 59
XbaI T'CTAG_A
Cuts at: 5209 7654 5209
Size: 2445 7873
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3952 4831 5113 5710 2011
Size: 1941 879 282 597 6619
Fragments arranged by size:
6619 1941 879 597 282
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10318
XmaI C'CCGG_G
Cuts at: 1870 7643 1870
Size: 5773 4545
XmnI GAAnn'nnTTC
Cuts at: 2069 3218 5303 7163 7502 8447 2069
Size: 1149 2085 1860 339 945 3940
Fragments arranged by size:
3940 2085 1860 1149 945 339
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7444 8126 276
Size: 53 83 186 936 5910 682 2468
Fragments arranged by size:
5910 2468 936 682 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI
SrfI SwaI