Restriction map for
pFN21AA0175
(Circular) (Six-base) MAPSORT of: pFN21AA0175.seq Check: 1645 from: 1 to: 6658
pFN21AA0175 6658 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2728 3259 2728
Size: 531 6127
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4468 278
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4001 1937
Size: 2064 4594
Acc65I G'GTAC_C
Cuts at: 3979 3979
Size: 6658
AclI AA'CG_TT
Cuts at: 1563 4785 5158 1563
Size: 3222 373 3063
Fragments arranged by size:
3222 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3812 4721 6105 6373 1632
Size: 2180 909 1384 268 1917
Fragments arranged by size:
2180 1917 1384 909 268
AflII C'TTAA_G
Cuts at: 820 1017 3896 820
Size: 197 2879 3582
Fragments arranged by size:
3582 2879 197
AflIII A'CryG_T
Cuts at: 1203 5557 6534 1203
Size: 4354 977 1327
Fragments arranged by size:
4354 1327 977
AgeI A'CCGG_T
Cuts at: 5489 5489
Size: 6658
AhdI GACnn_n'nnGTC
Cuts at: 3055 5387 3055
Size: 2332 4326
AleI CACnn'nnGTG
Cuts at: 1166 3793 1166
Size: 2627 4031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6658
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2976 3008 2976
Size: 32 6626
AlwNI CAG_nnn'CTG
Cuts at: 2534 3158 3427 4401 5973 2534
Size: 624 269 974 1572 3219
Fragments arranged by size:
3219 1572 974 624 269
ApaI G_GGCC'C
Cuts at: 3666 3666
Size: 6658
ApaLI G'TGCA_C
Cuts at: 2372 4715 5871 2372
Size: 2343 1156 3159
Fragments arranged by size:
3159 2343 1156
ApoI r'AATT_y
Cuts at: 1493 2413 3457 3967 4193 4345 6454 1493
Size: 920 1044 510 226 152 2109 1697
Fragments arranged by size:
2109 1697 1044 920 510 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3116 5212 160
Size: 872 2084 2096 1606
Fragments arranged by size:
2096 2084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2979 3983 1870
Size: 73 388 648 1004 4545
Fragments arranged by size:
4545 1004 648 388 73
BamHI G'GATC_C
Cuts at: 3988 4355 3988
Size: 367 6291
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3510 3979 5434 618
Size: 325 217 627 1723 469 1455 1842
Fragments arranged by size:
1842 1723 1455 627 469 325 217
BanII G_rGCy'C
Cuts at: 1391 3666 3977 1391
Size: 2275 311 4072
Fragments arranged by size:
4072 2275 311
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2332 3676 3939 928
Size: 971 433 1344 263 3647
Fragments arranged by size:
3647 1344 971 433 263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4849 4883 1903
Size: 34 2912 34 3678
Fragments arranged by size:
3678 2912 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4550 5760 4550
Size: 1210 5448
BclI T'GATC_A
Cuts at: 1548 2935 1548
Size: 1387 5271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2975 5269 136
Size: 107 122 71 631 605 1303 2294 1525
Fragments arranged by size:
2294 1525 1303 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6653 6653
Size: 6658
BlpI GC'TnA_GC
Cuts at: 4075 4075
Size: 6658
Bme1580I G_kGCm'C
Cuts at: 1790 2376 3666 4719 5875 1790
Size: 586 1290 1053 1156 2573
Fragments arranged by size:
2573 1290 1156 1053 586
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6658
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2700 4389 5347
Size: 416 53 83 399 24 1283 1689 958
Cuts at: 5347 442
Size: 1753
Fragments arranged by size:
1753 1689 1283 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6658
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3392
Size: 6658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2124 2295 3216 3327 5318 1239
Size: 885 171 921 111 1991 2579
Fragments arranged by size:
2579 1991 921 885 171 111
Bpu10I CC'TnA_GC
Cuts at: 3330 3330
Size: 6658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2387 4783 5648 5946 2282
Size: 105 2396 865 298 2994
Fragments arranged by size:
2994 2396 865 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2321 2465 5321 882
Size: 617 822 144 2856 2219
Fragments arranged by size:
2856 2219 822 617 144
BsaAI yAC'GTr
Cuts at: 493 1204 4449 493
Size: 711 3245 2702
Fragments arranged by size:
3245 2702 711
BsaBI GATnn'nnATC
Cuts at: 2430 2493 6652 2430
Size: 63 4159 2436
Fragments arranged by size:
4159 2436 63
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4465 4847 5482 275
Size: 53 83 186 936 2932 382 635 1451
Fragments arranged by size:
2932 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5091 5489 5763 5910 6321 5091
Size: 398 274 147 411 5428
Fragments arranged by size:
5428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2097 2127 3564 3594 1178
Size: 30 165 30 694 30 1437 30 4242
Fragments arranged by size:
4242 1437 694 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2292 2339 3566 1186
Size: 1106 47 1227 4278
Fragments arranged by size:
4278 1227 1106 47
BseYI C'CCAG_C
Cuts at: 5861 6357 5861
Size: 496 6162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6085
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4108 4869 5897 1540
Size: 275 186 2107 761 1028 2301
Fragments arranged by size:
2301 2107 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2376 3627 4719 4804 5875 2376
Size: 1251 1092 85 1071 3159
Fragments arranged by size:
3159 1251 1092 1071 85
BsmI GAATG_Cn'
Cuts at: 2712 4169 4262 2712
Size: 1457 93 5108
Fragments arranged by size:
5108 1457 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3651 3651
Size: 6658
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2376 3627 3666 3977 4719 4804 5875
Size: 399 586 1251 39 311 742 85 1071
Cuts at: 5875 1391
Size: 2174
Fragments arranged by size:
2174 1251 1071 742 586 399 311 85
39
BspEI T'CCGG_A
Cuts at: 6321 6321
Size: 6658
BspHI T'CATG_A
Cuts at: 4547 6277 4547
Size: 1730 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2728 3259 4013 844
Size: 1884 531 754 3489
Fragments arranged by size:
3489 1884 754 531
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 4545 1394
Size: 429 1693 1029 3507
Fragments arranged by size:
3507 1693 1029 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5153 5327 65
Size: 1183 3905 174 1396
Fragments arranged by size:
3905 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5302 5489 1700
Size: 110 3492 187 2869
Fragments arranged by size:
3492 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2878 3772 96
Size: 2782 894 2982
Fragments arranged by size:
2982 2782 894
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6658
BssSI C'ACGA_G
Cuts at: 4718 5730 4718
Size: 1012 5646
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6658
BstBI TT'CG_AA
Cuts at: 5462 5462
Size: 6658
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6658
BstYI r'GATC_y
Cuts at: 1972 3529 3988 4355 4740 4757 6209 6295 6307
Size: 1557 459 367 385 17 1452 86 12
Cuts at: 6307 6653 1972
Size: 346 1977
Fragments arranged by size:
1977 1557 1452 459 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3918 487
Size: 683 56 2692 3227
Fragments arranged by size:
3227 2692 683 56
BtsI GCAGTG_nn'
Cuts at: 4174 4968 4988 4174
Size: 794 20 5844
Fragments arranged by size:
5844 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3192 3227 529
Size: 35 2628 35 3960
Fragments arranged by size:
3960 2628 35 35
DraI TTT'AAA
Cuts at: 3962 3962
Size: 6658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5665 809
Size: 507 4349 1802
Fragments arranged by size:
4349 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4105 4994 6623
Size: 54 1425 50 275 18 2275 889 1629
Cuts at: 6623 8
Size: 43
Fragments arranged by size:
2275 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4105 1537
Size: 275 2293 4090
Fragments arranged by size:
4090 2293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 3306 4588 1353
Size: 963 990 1282 3423
Fragments arranged by size:
3423 1282 990 963
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5245 5619 5765 387
Size: 1064 138 3656 374 146 1280
Fragments arranged by size:
3656 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2295 3216 3327 3812 4721 5318
Size: 393 492 171 921 111 485 909 597
Cuts at: 5318 6105 6373 1239
Size: 787 268 1524
Fragments arranged by size:
1524 921 909 787 597 492 485 393
268 171 111
EcoO109I rG'GnC_Cy
Cuts at: 2398 2398
Size: 6658
EcoRI G'AATT_C
Cuts at: 3967 4345 3967
Size: 378 6280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3024 3024
Size: 6658
FspI TGC'GCA
Cuts at: 5164 5164
Size: 6658
HaeII r_GCGC'y
Cuts at: 1678 5805 1678
Size: 4127 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 3041 3073 3131
Size: 32 135 32 728 32 709 32 58
Cuts at: 3131 3163 3926 3958 5299 5331 5373 5405 1373
Size: 32 763 32 1341 32 42 32 2626
Fragments arranged by size:
2626 1341 763 728 709 135 58 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2216 3766 4002 4248 669
Size: 988 281 278 1550 236 246 3079
Fragments arranged by size:
3079 1550 988 281 278 246 236
HindIII A'AGCT_T
Cuts at: 748 3677 748
Size: 2929 3729
HpaI GTT'AAC
Cuts at: 4248 4248
Size: 6658
KpnI G_GTAC'C
Cuts at: 3983 3983
Size: 6658
MfeI C'AATT_G
Cuts at: 4257 4257
Size: 6658
MluI A'CGCG_T
Cuts at: 6534 6534
Size: 6658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3585 5772 5956 1305
Size: 2280 2187 184 2007
Fragments arranged by size:
2280 2187 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4836
Fragments arranged by size:
4836 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2850 3687 3793 4616 4975 5134 518
Size: 648 1684 837 106 823 359 159 2042
Fragments arranged by size:
2042 1684 837 823 648 359 159 106
MspA1I CmG'CkG
Cuts at: 1606 3892 4074 4751 5899 6144 1606
Size: 2286 182 677 1148 245 2120
Fragments arranged by size:
2286 2120 1148 677 245 182
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6106
NdeI CA'TA_TG
Cuts at: 387 3546 387
Size: 3159 3499
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6658
NotI GC'GGCC_GC
Cuts at: 4105 4105
Size: 6658
NspI r_CATG'y
Cuts at: 1132 1229 2508 3764 4016 5561 1132
Size: 97 1279 1256 252 1545 2229
Fragments arranged by size:
2229 1545 1279 1256 252 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6658
PciI A'CATG_T
Cuts at: 5557 5557
Size: 6658
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3192 1108
Size: 2084 4574
PmeI GTTT'AAAC
Cuts at: 3962 3962
Size: 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4727 4759 6240 6272 4727
Size: 32 1481 32 5113
Fragments arranged by size:
5113 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2398 2398
Size: 6658
PshAI GACnn'nnGTC
Cuts at: 1613 2457 3488 1613
Size: 844 1031 4783
Fragments arranged by size:
4783 1031 844
PsiI TTA'TAA
Cuts at: 4228 4228
Size: 6658
PspOMI G'GGCC_C
Cuts at: 3662 3662
Size: 6658
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2862 2894 2862
Size: 32 6626
PstI C_TGCA'G
Cuts at: 830 2739 4010 830
Size: 1909 1271 3478
Fragments arranged by size:
3478 1909 1271
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6658
SalI G'TCGA_C
Cuts at: 1936 4000 1936
Size: 2064 4594
SbfI CC_TGCA'GG
Cuts at: 4010 4010
Size: 6658
ScaI AGT'ACT
Cuts at: 2267 3191 3317 4906 6618 2267
Size: 924 126 1589 1712 2307
Fragments arranged by size:
2307 1712 1589 924 126
SfcI C'TryA_G
Cuts at: 826 1045 2498 2735 4006 5141 5822 6013 826
Size: 219 1453 237 1271 1135 681 191 1471
Fragments arranged by size:
1471 1453 1271 1135 681 237 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6658
SmaI CCC'GGG
Cuts at: 1872 2333 3985 1872
Size: 461 1652 4545
Fragments arranged by size:
4545 1652 461
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 2402 3896 4762 5663 5925
Size: 197 926 318 141 1494 866 901 262
Cuts at: 5925 820
Size: 1553
Fragments arranged by size:
1553 1494 926 901 866 318 262 197
141
SnaBI TAC'GTA
Cuts at: 493 4449 493
Size: 3956 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6658
SphI G_CATG'C
Cuts at: 1132 4016 1132
Size: 2884 3774
SspI AAT'ATT
Cuts at: 5 52 2033 2250 4582 5
Size: 47 1981 217 2332 2081
Fragments arranged by size:
2332 2081 1981 217 47
StuI AGG'CCT
Cuts at: 1517 2183 1517
Size: 666 5992
StyI C'CwwG_G
Cuts at: 513 1065 2475 4097 513
Size: 552 1410 1622 3074
Fragments arranged by size:
3074 1622 1410 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4685 4702 4855 5040 5485 325
Size: 1255 3105 17 153 185 445 1498
Fragments arranged by size:
3105 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 2878 3189
Size: 274 80 33 51 1443 288 613 311
Cuts at: 3189 3315 3772 4904 6616 96
Size: 126 457 1132 1712 138
Fragments arranged by size:
1712 1443 1132 613 457 311 288 274
138 126 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3564 3596 6618 6650 3564
Size: 32 3022 32 3572
Fragments arranged by size:
3572 3022 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6658
XbaI T'CTAG_A
Cuts at: 3994 3994
Size: 6658
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 2099
Size: 6658
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6658
XmaI C'CCGG_G
Cuts at: 1870 2331 3983 1870
Size: 461 1652 4545
Fragments arranged by size:
4545 1652 461
XmnI GAAnn'nnTTC
Cuts at: 4787 4787
Size: 6658
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4466 276
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI