Restriction map for
pFN21AA0164
(Circular) (Six-base) MAPSORT of: pFN21AA0164.seq Check: 7915 from: 1 to: 7465
pFN21AA0164 7465 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5275 278
Size: 53 83 186 936 3739 2468
Fragments arranged by size:
3739 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3578 4808 1937
Size: 1641 1230 4594
Fragments arranged by size:
4594 1641 1230
Acc65I G'GTAC_C
Cuts at: 4786 4786
Size: 7465
AclI AA'CG_TT
Cuts at: 1563 5592 5965 1563
Size: 4029 373 3063
Fragments arranged by size:
4029 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 3139 3568 3660 3792 3942 4251 5528
Size: 900 607 429 92 132 150 309 1277
Cuts at: 5528 6912 7180 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1277 900 607 429 309 268
150 132 92
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7268
AflIII A'CryG_T
Cuts at: 1203 6364 7341 1203
Size: 5161 977 1327
Fragments arranged by size:
5161 1327 977
AgeI A'CCGG_T
Cuts at: 6296 6296
Size: 7465
AhdI GACnn_n'nnGTC
Cuts at: 6194 6194
Size: 7465
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7465
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7465
AlwNI CAG_nnn'CTG
Cuts at: 5208 6780 5208
Size: 1572 5893
ApaLI G'TGCA_C
Cuts at: 5522 6678 5522
Size: 1156 6309
ApoI r'AATT_y
Cuts at: 1493 2606 3454 3755 4331 4774 5000 5152 7261
Size: 1113 848 301 576 443 226 152 2109
Cuts at: 7261 1493
Size: 1697
Fragments arranged by size:
2109 1697 1113 848 576 443 301 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6019 160
Size: 872 4987 1606
Fragments arranged by size:
4987 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7465
AvaI C'yCGr_G
Cuts at: 1870 1943 4293 4790 1870
Size: 73 2350 497 4545
Fragments arranged by size:
4545 2350 497 73
BamHI G'GATC_C
Cuts at: 4510 4795 5162 4510
Size: 285 367 6813
Fragments arranged by size:
6813 367 285
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3658 4786 6241 618
Size: 325 217 627 1871 1128 1455 1842
Fragments arranged by size:
1871 1842 1455 1128 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3950 4145 4784 1391
Size: 2559 195 639 4072
Fragments arranged by size:
4072 2559 639 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3453 928
Size: 971 1554 4940
Fragments arranged by size:
4940 1554 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5656 5690 1903
Size: 34 3719 34 3678
Fragments arranged by size:
3719 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5357 6567 5357
Size: 1210 6255
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7465
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6076 136
Size: 107 122 71 631 605 4404 1525
Fragments arranged by size:
4404 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2081 2387 2405 7460 2081
Size: 306 18 5055 2086
Fragments arranged by size:
5055 2086 306 18
BlpI GC'TnA_GC
Cuts at: 4882 4882
Size: 7465
Bme1580I G_kGCm'C
Cuts at: 1790 5526 6682 1790
Size: 3736 1156 2573
Fragments arranged by size:
3736 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7465
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 4281 5196
Size: 416 53 83 399 24 1595 1269 915
Cuts at: 5196 6154 442
Size: 958 1753
Fragments arranged by size:
1753 1595 1269 958 915 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7465
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3645 3645
Size: 7465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4129 6125 1239
Size: 2890 1996 2579
Fragments arranged by size:
2890 2579 1996
Bpu10I CC'TnA_GC
Cuts at: 3495 3495
Size: 7465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3296 5590 6455 6753 3296
Size: 2294 865 298 4008
Fragments arranged by size:
4008 2294 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2121 2145 2421 2490 4483 4706 6128
Size: 617 622 24 276 69 1993 223 1422
Cuts at: 6128 882
Size: 2219
Fragments arranged by size:
2219 1993 1422 622 617 276 223 69
24
BsaAI yAC'GTr
Cuts at: 493 1204 5256 493
Size: 711 4052 2702
Fragments arranged by size:
4052 2702 711
BsaBI GATnn'nnATC
Cuts at: 2074 2080 4258 7459 2074
Size: 6 2178 3201 2080
Fragments arranged by size:
3201 2178 2080 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5272 5654 6289 275
Size: 53 83 186 936 3739 382 635 1451
Fragments arranged by size:
3739 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2630 3288 5898 6296 6570 6717 7128 2630
Size: 658 2610 398 274 147 411 2967
Fragments arranged by size:
2967 2610 658 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7240
Fragments arranged by size:
7240 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2268 2271 3227 3314 4446 1186
Size: 1082 3 956 87 1132 4205
Fragments arranged by size:
4205 1132 1082 956 87 3
BseYI C'CCAG_C
Cuts at: 4442 6668 7164 4442
Size: 2226 496 4743
Fragments arranged by size:
4743 2226 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6892
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4915 5676 6704 1540
Size: 275 186 2914 761 1028 2301
Fragments arranged by size:
2914 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5526 5611 6682 5526
Size: 85 1071 6309
Fragments arranged by size:
6309 1071 85
BsmI GAATG_Cn'
Cuts at: 2785 2935 4976 5069 2785
Size: 150 2041 93 5181
Fragments arranged by size:
5181 2041 150 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2200 2200
Size: 7465
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3950 4145 4784 5526 5611 6682 1391
Size: 399 2160 195 639 742 85 1071 2174
Fragments arranged by size:
2174 2160 1071 742 639 399 195 85
BspEI T'CCGG_A
Cuts at: 7128 7128
Size: 7465
BspHI T'CATG_A
Cuts at: 4669 5354 7084 4669
Size: 685 1730 5050
Fragments arranged by size:
5050 1730 685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3490 3697 4820 844
Size: 2646 207 1123 3489
Fragments arranged by size:
3489 2646 1123 207
BsrBI CCG'CTC
Cuts at: 1394 1823 3895 5352 1394
Size: 429 2072 1457 3507
Fragments arranged by size:
3507 2072 1457 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3885 5960 6134 65
Size: 1183 2637 2075 174 1396
Fragments arranged by size:
2637 2075 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2394 6109 6296 1700
Size: 110 584 3715 187 2869
Fragments arranged by size:
3715 2869 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7465
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7465
BssSI C'ACGA_G
Cuts at: 3567 4389 5525 6537 3567
Size: 822 1136 1012 4495
Fragments arranged by size:
4495 1136 1012 822
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7465
BstBI TT'CG_AA
Cuts at: 4405 6269 4405
Size: 1864 5601
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7465
BstXI CCAn_nnnn'nTGG
Cuts at: 4688 4688
Size: 7465
BstYI r'GATC_y
Cuts at: 1972 2081 2357 2375 2387 2405 2843 4510 4795
Size: 109 276 18 12 18 438 1667 285
Cuts at: 4795 5162 5547 5564 7016 7102 7114 7460 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1667 1452 438 385 367 346 285
276 109 86 18 18 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 3989 3989
Size: 7465
BtgI C'CryG_G
Cuts at: 513 1065 2004 4716 513
Size: 552 939 2712 3262
Fragments arranged by size:
3262 2712 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6726
Fragments arranged by size:
6726 683 56
BtsI GCAGTG_nn'
Cuts at: 4981 5775 5795 4981
Size: 794 20 6651
Fragments arranged by size:
6651 794 20
ClaI AT'CG_AT
Cuts at: 1554 2601 1554
Size: 1047 6418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7430
DraI TTT'AAA
Cuts at: 4030 4336 4769 4030
Size: 306 433 6726
Fragments arranged by size:
6726 433 306
DraIII CAC_nnn'GTG
Cuts at: 3832 3832
Size: 7465
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6472 809
Size: 507 5156 1802
Fragments arranged by size:
5156 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4912 5801 7430
Size: 54 1425 50 275 18 3082 889 1629
Cuts at: 7430 8
Size: 43
Fragments arranged by size:
3082 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4912 1537
Size: 275 3100 4090
Fragments arranged by size:
4090 3100 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2437 2523 3027 4011 4270 4417 4494 4588
Size: 1084 86 504 984 259 147 77 94
Cuts at: 4588 4704 5395 1353
Size: 116 691 3423
Fragments arranged by size:
3423 1084 984 691 504 259 147 116
94 86 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6052 6426 6572 387
Size: 1064 138 4463 374 146 1280
Fragments arranged by size:
4463 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 3139 3568 3660 3792 3942 4129
Size: 393 900 607 429 92 132 150 187
Cuts at: 4129 4251 5528 6125 6912 7180 1239
Size: 122 1277 597 787 268 1524
Fragments arranged by size:
1524 1277 900 787 607 597 429 393
268 187 150 132 122 92
EcoNI CCTnn'n_nnAGG
Cuts at: 2322 2322
Size: 7465
EcoRI G'AATT_C
Cuts at: 3755 4774 5152 3755
Size: 1019 378 6068
Fragments arranged by size:
6068 1019 378
EcoRV GAT'ATC
Cuts at: 2267 2267
Size: 7465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3598 4558 4561 3598
Size: 960 3 6502
Fragments arranged by size:
6502 960 3
FspI TGC'GCA
Cuts at: 5971 5971
Size: 7465
HaeII r_GCGC'y
Cuts at: 1678 4630 6612 1678
Size: 2952 1982 2531
Fragments arranged by size:
2952 2531 1982
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3020 3052 3083 3115 3152
Size: 32 135 32 1448 32 31 32 37
Cuts at: 3152 3184 6106 6138 6180 6212 1373
Size: 32 2922 32 42 32 2626
Fragments arranged by size:
2922 2626 1448 135 42 37 32 32
32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 4809 5055 669
Size: 988 281 2871 246 3079
Fragments arranged by size:
3079 2871 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7465
HpaI GTT'AAC
Cuts at: 5055 5055
Size: 7465
KpnI G_GTAC'C
Cuts at: 4790 4790
Size: 7465
MfeI C'AATT_G
Cuts at: 5064 5064
Size: 7465
MluI A'CGCG_T
Cuts at: 7341 7341
Size: 7465
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2815 6579 6763 1305
Size: 1510 3764 184 2007
Fragments arranged by size:
3764 2007 1510 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5643
Fragments arranged by size:
5643 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 5423 5782 5941 518
Size: 648 1108 3149 359 159 2042
Fragments arranged by size:
3149 2042 1108 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3875 4881 5558 6706 6951 1606
Size: 2269 1006 677 1148 245 2120
Fragments arranged by size:
2269 2120 1148 1006 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4716 513
Size: 552 939 2712 3262
Fragments arranged by size:
3262 2712 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7465
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7465
NotI GC'GGCC_GC
Cuts at: 4912 4912
Size: 7465
NruI TCG'CGA
Cuts at: 2181 2181
Size: 7465
NspI r_CATG'y
Cuts at: 1132 1229 4823 6368 1132
Size: 97 3594 1545 2229
Fragments arranged by size:
3594 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7465
PciI A'CATG_T
Cuts at: 6364 6364
Size: 7465
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7465
PfoI T'CCnGG_A
Cuts at: 2339 2339
Size: 7465
PmeI GTTT'AAAC
Cuts at: 4769 4769
Size: 7465
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5534 5566 7047 7079 5534
Size: 32 1481 32 5920
Fragments arranged by size:
5920 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2482 1613
Size: 869 6596
PsiI TTA'TAA
Cuts at: 3381 5035 3381
Size: 1654 5811
PstI C_TGCA'G
Cuts at: 830 4817 830
Size: 3987 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7465
PvuII CAG'CTG
Cuts at: 3875 3875
Size: 7465
SalI G'TCGA_C
Cuts at: 1936 4807 1936
Size: 2871 4594
SapI GCTCTTCn'nnn_
Cuts at: 4417 4417
Size: 7465
SbfI CC_TGCA'GG
Cuts at: 4817 4817
Size: 7465
ScaI AGT'ACT
Cuts at: 3934 4541 5713 7425 3934
Size: 607 1172 1712 3974
Fragments arranged by size:
3974 1712 1172 607
SfcI C'TryA_G
Cuts at: 826 1045 4813 5948 6629 6820 826
Size: 219 3768 1135 681 191 1471
Fragments arranged by size:
3768 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7465
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7465
SmaI CCC'GGG
Cuts at: 1872 4792 1872
Size: 2920 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3311 4293 5569 6470 6732 820
Size: 197 926 1368 982 1276 901 262 1553
Fragments arranged by size:
1553 1368 1276 982 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5256 493
Size: 4763 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7465
SphI G_CATG'C
Cuts at: 1132 4823 1132
Size: 3691 3774
SspI AAT'ATT
Cuts at: 5 52 5389 5
Size: 47 5337 2081
Fragments arranged by size:
5337 2081 47
StuI AGG'CCT
Cuts at: 1517 3415 1517
Size: 1898 5567
StyI C'CwwG_G
Cuts at: 513 1065 2004 3837 4137 4681 4716 4904 513
Size: 552 939 1833 300 544 35 188 3074
Fragments arranged by size:
3074 1833 939 552 544 300 188 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4507 5492 5509 5662 5847 6292 325
Size: 1255 2927 985 17 153 185 445 1498
Fragments arranged by size:
2927 1498 1255 985 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2750 3315 3722
Size: 274 80 33 51 1443 773 565 407
Cuts at: 3722 3932 4539 5711 7423 96
Size: 210 607 1172 1712 138
Fragments arranged by size:
1712 1443 1172 773 607 565 407 274
210 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3390 3422 3818 3850 7425 7457 3390
Size: 32 396 32 3575 32 3398
Fragments arranged by size:
3575 3398 396 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2325 1316
Size: 1009 6456
XbaI T'CTAG_A
Cuts at: 2021 2048 2090 2165 3038 3145 4801 2021
Size: 27 42 75 873 107 1656 4685
Fragments arranged by size:
4685 1656 873 107 75 42 27
XhoI C'TCGA_G
Cuts at: 1943 4293 1943
Size: 2350 5115
XmaI C'CCGG_G
Cuts at: 1870 4790 1870
Size: 2920 4545
XmnI GAAnn'nnTTC
Cuts at: 2131 4541 5594 2131
Size: 2410 1053 4002
Fragments arranged by size:
4002 2410 1053
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5273 276
Size: 53 83 186 936 3739 2468
Fragments arranged by size:
3739 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstZ17I EcoICRI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI