Restriction map for
pFN21AA0159
(Circular) (Six-base) MAPSORT of: pFN21AA0159.seq Check: 8964 from: 1 to: 8908
pFN21AA0159 8908 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2688 4435 2688
Size: 1747 7161
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 6718 278
Size: 53 83 186 936 1705 3477 2468
Fragments arranged by size:
3477 2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 3758 6251 1937
Size: 1530 291 2493 4594
Fragments arranged by size:
4594 2493 1530 291
Acc65I G'GTAC_C
Cuts at: 4006 5976 6229 4006
Size: 1970 253 6685
Fragments arranged by size:
6685 1970 253
AclI AA'CG_TT
Cuts at: 1563 7035 7408 1563
Size: 5472 373 3063
Fragments arranged by size:
5472 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 2519 2871 3564 3634 5767 6971 8355
Size: 866 21 352 693 70 2133 1204 1384
Cuts at: 8355 8623 1632
Size: 268 1917
Fragments arranged by size:
2133 1917 1384 1204 866 693 352 268
70 21
AfeI AGC'GCT
Cuts at: 4316 4316
Size: 8908
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8711
AflIII A'CryG_T
Cuts at: 1203 2932 3203 7807 8784 1203
Size: 1729 271 4604 977 1327
Fragments arranged by size:
4604 1729 1327 977 271
AgeI A'CCGG_T
Cuts at: 7739 7739
Size: 8908
AhdI GACnn_n'nnGTC
Cuts at: 3785 5764 7637 3785
Size: 1979 1873 5056
Fragments arranged by size:
5056 1979 1873
AleI CACnn'nnGTG
Cuts at: 1166 4391 1166
Size: 3225 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4698 1184
Size: 3514 5394
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4769 4801 4769
Size: 32 8876
AlwNI CAG_nnn'CTG
Cuts at: 2746 3286 3413 3874 4018 4270 4696 4816 5035
Size: 540 127 461 144 252 426 120 219
Cuts at: 5035 5986 6651 8223 2746
Size: 951 665 1572 3431
Fragments arranged by size:
3431 1572 951 665 540 461 426 252
219 144 127 120
ApaI G_GGCC'C
Cuts at: 2163 3391 4649 2163
Size: 1228 1258 6422
Fragments arranged by size:
6422 1258 1228
ApaLI G'TGCA_C
Cuts at: 6965 8121 6965
Size: 1156 7752
ApoI r'AATT_y
Cuts at: 1493 2252 2582 3977 4215 5855 6217 6443 6595
Size: 759 330 1395 238 1640 362 226 152
Cuts at: 6595 8704 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1640 1395 759 362 330 238
226 152
AseI AT'TA_AT
Cuts at: 160 1032 2637 7462 160
Size: 872 1605 4825 1606
Fragments arranged by size:
4825 1606 1605 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8908
AvaI C'yCGr_G
Cuts at: 1870 1943 2661 3078 3612 3750 5426 5534 6233
Size: 73 718 417 534 138 1676 108 699
Cuts at: 6233 1870
Size: 4545
Fragments arranged by size:
4545 1676 718 699 534 417 138 108
73
BamHI G'GATC_C
Cuts at: 3814 4596 6238 6605 3814
Size: 782 1642 367 6117
Fragments arranged by size:
6117 1642 782 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2767 4006 4857 5976 6229
Size: 325 217 627 980 1239 851 1119 253
Cuts at: 6229 7684 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1239 1119 980 851 627 325
253 217
BanII G_rGCy'C
Cuts at: 1391 2155 2163 3391 4649 4851 4922 5472 6030
Size: 764 8 1228 1258 202 71 550 558
Cuts at: 6030 6227 1391
Size: 197 4072
Fragments arranged by size:
4072 1258 1228 764 558 550 202 197
71 8
BbeI G_GCGC'C
Cuts at: 4861 4861
Size: 8908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3985 5168 6088 6153 928
Size: 971 2086 1183 920 65 3683
Fragments arranged by size:
3683 2086 1183 971 920 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2195 2229 5852 5886 7099 7133 1903
Size: 34 258 34 3623 34 1213 34 3678
Fragments arranged by size:
3678 3623 1213 258 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2281 3097 6800 8010 2281
Size: 816 3703 1210 3179
Fragments arranged by size:
3703 3179 1210 816
BclI T'GATC_A
Cuts at: 1548 5142 1548
Size: 3594 5314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7519 136
Size: 107 122 71 631 605 5847 1525
Fragments arranged by size:
5847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6124 8903 6124
Size: 2779 6129
BlpI GC'TnA_GC
Cuts at: 5319 6325 5319
Size: 1006 7902
Bme1580I G_kGCm'C
Cuts at: 1790 2163 3391 4649 4666 6969 8125 1790
Size: 373 1228 1258 17 2303 1156 2573
Fragments arranged by size:
2573 2303 1258 1228 1156 373 17
BmgBI CAC'GTC
Cuts at: 1318 5819 1318
Size: 4501 4407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2497 5250 6639
Size: 416 53 83 399 24 1080 2753 1389
Cuts at: 6639 7597 442
Size: 958 1753
Fragments arranged by size:
2753 1753 1389 1080 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2642 4331 4718 7568 1239
Size: 1403 1689 387 2850 2579
Fragments arranged by size:
2850 2579 1689 1403 387
Bpu10I CC'TnA_GC
Cuts at: 2154 3516 2154
Size: 1362 7546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4229 5351 5933 6085 7033 7898 8196 4229
Size: 1122 582 152 948 865 298 4941
Fragments arranged by size:
4941 1122 948 865 582 298 152
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2393 2874 3084 3356 3674 4919 5670
Size: 617 894 481 210 272 318 1245 751
Cuts at: 5670 7571 882
Size: 1901 2219
Fragments arranged by size:
2219 1901 1245 894 751 617 481 318
272 210
BsaAI yAC'GTr
Cuts at: 493 1204 6699 493
Size: 711 5495 2702
Fragments arranged by size:
5495 2702 711
BsaBI GATnn'nnATC
Cuts at: 8902 8902
Size: 8908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3238 4858 6715 7097
Size: 53 83 186 936 1705 1620 1857 382
Cuts at: 7097 7732 275
Size: 635 1451
Fragments arranged by size:
1857 1705 1620 1451 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5217 7341 7739 8013 8160 8571 5217
Size: 2124 398 274 147 411 5554
Fragments arranged by size:
5554 2124 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2823 2853 3878 3908 1178
Size: 30 165 30 1420 30 1025 30 6178
Fragments arranged by size:
6178 1420 1025 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 3446 3449 3518 3521 3683 3692 4952
Size: 877 1383 3 69 3 162 9 1260
Cuts at: 4952 4955 5277 5573 1186
Size: 3 322 296 4521
Fragments arranged by size:
4521 1383 1260 877 322 296 162 69
9 3 3 3
BseYI C'CCAG_C
Cuts at: 2938 3721 4263 4440 4793 4945 5166 5539 6060
Size: 783 542 177 353 152 221 373 521
Cuts at: 6060 8111 8607 2938
Size: 2051 496 3239
Fragments arranged by size:
3239 2051 783 542 521 496 373 353
221 177 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3983 4253 1163
Size: 573 2247 270 5818
Fragments arranged by size:
5818 2247 573 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6358 7119 8147 1540
Size: 275 186 4357 761 1028 2301
Fragments arranged by size:
4357 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2155 4851 4887 4922 5472 6969 7054 8125 2155
Size: 2696 36 35 550 1497 85 1071 2938
Fragments arranged by size:
2938 2696 1497 1071 550 85 36 35
BsmI GAATG_Cn'
Cuts at: 2909 6419 6512 2909
Size: 3510 93 5305
Fragments arranged by size:
5305 3510 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2387 2387
Size: 8908
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2163 3391 4649 4666 4851 4887
Size: 399 365 8 1228 1258 17 185 36
Cuts at: 4887 4922 5472 6030 6227 6969 7054 8125 1391
Size: 35 550 558 197 742 85 1071 2174
Fragments arranged by size:
2174 1258 1228 1071 742 558 550 399
365 197 185 85 36 35 17 8
BspEI T'CCGG_A
Cuts at: 8571 8571
Size: 8908
BspHI T'CATG_A
Cuts at: 5571 6797 8527 5571
Size: 1226 1730 5952
Fragments arranged by size:
5952 1730 1226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 3034 4435 4788 5383 5918 6263 844
Size: 1844 346 1401 353 595 535 345 3489
Fragments arranged by size:
3489 1844 1401 595 535 353 346 345
BsrBI CCG'CTC
Cuts at: 1394 1823 4279 5665 6795 1394
Size: 429 2456 1386 1130 3507
Fragments arranged by size:
3507 2456 1386 1130 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4458 4758 7403 7577 65
Size: 1183 3210 300 2645 174 1396
Fragments arranged by size:
3210 2645 1396 1183 300 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4854 7552 7739 1700
Size: 110 3044 2698 187 2869
Fragments arranged by size:
3044 2869 2698 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8908
BssSI C'ACGA_G
Cuts at: 4350 6968 7980 4350
Size: 2618 1012 5278
Fragments arranged by size:
5278 2618 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3413 3859 5136 794
Size: 2619 446 1277 4566
Fragments arranged by size:
4566 2619 1277 446
BstBI TT'CG_AA
Cuts at: 2215 7712 2215
Size: 5497 3411
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8908
BstXI CCAn_nnnn'nTGG
Cuts at: 5272 5893 5272
Size: 621 8287
BstYI r'GATC_y
Cuts at: 1972 2224 3814 4596 4897 5245 6124 6203 6238
Size: 252 1590 782 301 348 879 79 35
Cuts at: 6238 6605 6990 7007 8459 8545 8557 8903 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1590 1452 879 782 385 367 348
346 301 252 86 79 35 17 12
Bsu36I CC'TnA_GG
Cuts at: 3819 4524 5832 3819
Size: 705 1308 6895
Fragments arranged by size:
6895 1308 705
BtgI C'CryG_G
Cuts at: 513 1065 2004 2403 2476 3024 3118 4932 4993
Size: 552 939 399 73 548 94 1814 61
Cuts at: 4993 513
Size: 4428
Fragments arranged by size:
4428 1814 939 552 548 399 94 73
61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8169
Fragments arranged by size:
8169 683 56
BtsI GCAGTG_nn'
Cuts at: 3222 3350 3423 4263 4623 4842 6424 7218 7238
Size: 128 73 840 360 219 1582 794 20
Cuts at: 7238 3222
Size: 4892
Fragments arranged by size:
4892 1582 840 794 360 219 128 73
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2406 2441 3939 3974 529
Size: 35 1842 35 1498 35 5463
Fragments arranged by size:
5463 1842 1498 35 35 35
DraI TTT'AAA
Cuts at: 6212 6212
Size: 8908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2859 7915 809
Size: 507 1543 5056 1802
Fragments arranged by size:
5056 1802 1543 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2406 3121 3288
Size: 54 1425 50 275 18 576 715 167
Cuts at: 3288 5250 5448 5920 6355 7244 8873 8
Size: 1962 198 472 435 889 1629 43
Fragments arranged by size:
1962 1629 1425 889 715 576 472 435
275 198 167 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6355 1537
Size: 275 4543 4090
Fragments arranged by size:
4543 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2490 2813 3170 4704 4709 5547 6838 1353
Size: 1137 323 357 1534 5 838 1291 3423
Fragments arranged by size:
3423 1534 1291 1137 838 357 323 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3044 7495 7869 8015 387
Size: 1064 138 1455 4451 374 146 1280
Fragments arranged by size:
4451 1455 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2153 4849 4920 5470 2153
Size: 2696 71 550 5591
Fragments arranged by size:
5591 2696 550 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2498 2519 2642 2871 3564 3634 4331
Size: 393 866 21 123 229 693 70 697
Cuts at: 4331 4718 5767 6971 7568 8355 8623 1239
Size: 387 1049 1204 597 787 268 1524
Fragments arranged by size:
1524 1204 1049 866 787 697 693 597
393 387 268 229 123 70 21
EcoO109I rG'GnC_Cy
Cuts at: 2159 2160 3387 4015 4645 4646 4693 4732 5311
Size: 1 1227 628 630 1 47 39 579
Cuts at: 5311 2159
Size: 5756
Fragments arranged by size:
5756 1227 630 628 579 47 39 1
1
EcoRI G'AATT_C
Cuts at: 2252 3977 6217 6595 2252
Size: 1725 2240 378 4565
Fragments arranged by size:
4565 2240 1725 378
EcoRV GAT'ATC
Cuts at: 5512 5512
Size: 8908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4100 5476 4100
Size: 1376 7532
FspI TGC'GCA
Cuts at: 7414 7414
Size: 8908
HaeII r_GCGC'y
Cuts at: 1678 4318 4861 5945 8055 1678
Size: 2640 543 1084 2110 2531
Fragments arranged by size:
2640 2531 2110 1084 543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 3119 3151 3449
Size: 32 135 32 512 32 1003 32 298
Cuts at: 3449 3481 3771 3803 4144 4176 4708 4740 4882
Size: 32 290 32 341 32 532 32 142
Cuts at: 4882 4914 5275 5307 5750 5782 7549 7581 7623
Size: 32 361 32 443 32 1767 32 42
Cuts at: 7623 7655 1373
Size: 32 2626
Fragments arranged by size:
2626 1767 1003 532 512 443 361 341
298 290 142 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3133 6252 6498 669
Size: 988 281 1195 3119 246 3079
Fragments arranged by size:
3119 3079 1195 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3323 5867 748
Size: 2575 2544 3789
Fragments arranged by size:
3789 2575 2544
HpaI GTT'AAC
Cuts at: 6498 6498
Size: 8908
KasI G'GCGC_C
Cuts at: 4857 4857
Size: 8908
KpnI G_GTAC'C
Cuts at: 4010 5980 6233 4010
Size: 1970 253 6685
Fragments arranged by size:
6685 1970 253
MfeI C'AATT_G
Cuts at: 6507 6507
Size: 8908
MluI A'CGCG_T
Cuts at: 8784 8784
Size: 8908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 3792 4998 8022 8206 1305
Size: 1215 1272 1206 3024 184 2007
Fragments arranged by size:
3024 2007 1272 1215 1206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 3123 3290 5252 5450
Size: 54 1425 343 576 715 167 1962 198
Cuts at: 5450 5922 10
Size: 472 2996
Fragments arranged by size:
2996 1962 1425 715 576 472 343 198
167 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2346 2741 2982 3123 4391 5295 5570
Size: 648 1180 395 241 141 1268 904 275
Cuts at: 5570 6866 7225 7384 518
Size: 1296 359 159 2042
Fragments arranged by size:
2042 1296 1268 1180 904 648 395 359
275 241 159 141
MspA1I CmG'CkG
Cuts at: 1606 3283 4267 4816 5111 5339 5641 5701 6324
Size: 1677 984 549 295 228 302 60 623
Cuts at: 6324 7001 8149 8394 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1677 1148 984 677 623 549 302
295 245 228 60
NaeI GCC'GGC
Cuts at: 4856 4856
Size: 8908
NarI GG'CG_CC
Cuts at: 4858 4858
Size: 8908
NcoI C'CATG_G
Cuts at: 513 1065 2004 2403 2476 3024 3118 4932 513
Size: 552 939 399 73 548 94 1814 4489
Fragments arranged by size:
4489 1814 939 552 548 399 94 73
NdeI CA'TA_TG
Cuts at: 387 2712 387
Size: 2325 6583
NgoMIV G'CCGG_C
Cuts at: 4854 4854
Size: 8908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8908
NotI GC'GGCC_GC
Cuts at: 6355 6355
Size: 8908
NspI r_CATG'y
Cuts at: 1132 1229 2962 6266 7811 1132
Size: 97 1733 3304 1545 2229
Fragments arranged by size:
3304 2229 1733 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3496 1348
Size: 2148 6760
PciI A'CATG_T
Cuts at: 7807 7807
Size: 8908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3100 3217 3286 5966 5986 1108
Size: 1992 117 69 2680 20 4030
Fragments arranged by size:
4030 2680 1992 117 69 20
PfoI T'CCnGG_A
Cuts at: 2301 2385 3633 3896 4071 4869 5190 5307 2301
Size: 84 1248 263 175 798 321 117 5902
Fragments arranged by size:
5902 1248 798 321 263 175 117 84
PmeI GTTT'AAAC
Cuts at: 6212 6212
Size: 8908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6977 7009 8490 8522 6977
Size: 32 1481 32 7363
Fragments arranged by size:
7363 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4732 5311 4732
Size: 579 8329
PshAI GACnn'nnGTC
Cuts at: 1613 2547 1613
Size: 934 7974
PsiI TTA'TAA
Cuts at: 2706 6478 2706
Size: 3772 5136
PspOMI G'GGCC_C
Cuts at: 2159 3387 4645 2159
Size: 1228 1258 6422
Fragments arranged by size:
6422 1258 1228
PstI C_TGCA'G
Cuts at: 830 2178 2751 3045 3357 3406 3418 3477 3879
Size: 1348 573 294 312 49 12 59 402
Cuts at: 3879 4812 5110 6260 830
Size: 933 298 1150 3478
Fragments arranged by size:
3478 1348 1150 933 573 402 312 298
294 59 49 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8908
PvuII CAG'CTG
Cuts at: 3283 4267 4816 5111 5701 3283
Size: 984 549 295 590 6490
Fragments arranged by size:
6490 984 590 549 295
SacI G_AGCT'C
Cuts at: 2155 4851 4922 5472 2155
Size: 2696 71 550 5591
Fragments arranged by size:
5591 2696 550 71
SalI G'TCGA_C
Cuts at: 1936 6250 1936
Size: 4314 4594
SanDI GG'GwC_CC
Cuts at: 5311 5311
Size: 8908
SapI GCTCTTCn'nnn_
Cuts at: 2813 3170 5547 2813
Size: 357 2377 6174
Fragments arranged by size:
6174 2377 357
SbfI CC_TGCA'GG
Cuts at: 6260 6260
Size: 8908
ScaI AGT'ACT
Cuts at: 7156 8868 7156
Size: 1712 7196
SfcI C'TryA_G
Cuts at: 826 1045 2174 2194 2724 2747 3041 3353 3402
Size: 219 1129 20 530 23 294 312 49
Cuts at: 3402 3414 3473 3485 3759 3875 3886 3954 4429
Size: 12 59 12 274 116 11 68 475
Cuts at: 4429 4808 5086 5106 5994 6256 7391 8072 8263
Size: 379 278 20 888 262 1135 681 191
Cuts at: 8263 826
Size: 1471
Fragments arranged by size:
1471 1135 1129 888 681 530 475 379
312 294 278 274 262 219 191 116
68 59 49 23 20 20 12 12
11
SfoI GGC'GCC
Cuts at: 4859 4859
Size: 8908
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8908
SmaI CCC'GGG
Cuts at: 1872 3752 6235 1872
Size: 1880 2483 4545
Fragments arranged by size:
4545 2483 1880
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3612 4208 5366 5912 6100 7012
Size: 197 926 1669 596 1158 546 188 912
Cuts at: 7012 7913 8175 820
Size: 901 262 1553
Fragments arranged by size:
1669 1553 1158 926 912 901 596 546
262 197 188
SnaBI TAC'GTA
Cuts at: 493 6699 493
Size: 6206 2702
SpeI A'CTAG_T
Cuts at: 152 3844 152
Size: 3692 5216
SphI G_CATG'C
Cuts at: 1132 6266 1132
Size: 5134 3774
SspI AAT'ATT
Cuts at: 5 52 6832 5
Size: 47 6780 2081
Fragments arranged by size:
6780 2081 47
StuI AGG'CCT
Cuts at: 1517 5082 5721 1517
Size: 3565 639 4704
Fragments arranged by size:
4704 3565 639
StyI C'CwwG_G
Cuts at: 513 1065 2004 2403 2437 2466 2476 3024 3118
Size: 552 939 399 34 29 10 548 94
Cuts at: 3118 4137 4902 4932 5125 5598 6347 513
Size: 1019 765 30 193 473 749 3074
Fragments arranged by size:
3074 1019 939 765 749 552 548 473
399 193 94 34 30 29 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4596 6074 6172 6935 6952 7105 7290
Size: 1255 3016 1478 98 763 17 153 185
Cuts at: 7290 7735 325
Size: 445 1498
Fragments arranged by size:
3016 1498 1478 1255 763 445 185 153
98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2773 7154 8866
Size: 274 80 33 51 1443 796 4381 1712
Cuts at: 8866 96
Size: 138
Fragments arranged by size:
4381 1712 1443 796 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4581 4613 5618 5650 8868 8900 4581
Size: 32 1005 32 3218 32 4589
Fragments arranged by size:
4589 3218 1005 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6014 1316
Size: 4698 4210
XbaI T'CTAG_A
Cuts at: 6244 6244
Size: 8908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3031 3283 3435 4019 4590 5245 3031
Size: 252 152 584 571 655 6694
Fragments arranged by size:
6694 655 584 571 252 152
XhoI C'TCGA_G
Cuts at: 1943 3612 1943
Size: 1669 7239
XmaI C'CCGG_G
Cuts at: 1870 3750 6233 1870
Size: 1880 2483 4545
Fragments arranged by size:
4545 2483 1880
XmnI GAAnn'nnTTC
Cuts at: 4486 5459 7037 4486
Size: 973 1578 6357
Fragments arranged by size:
6357 1578 973
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 6716 276
Size: 53 83 186 936 1705 3477 2468
Fragments arranged by size:
3477 2468 1705 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I
DraIII EcoNI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI