Restriction map for
pFN21AA0156
(Circular) (Six-base) MAPSORT of: pFN21AA0156.seq Check: 6946 from: 1 to: 7594
pFN21AA0156 7594 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5404 278
Size: 53 83 186 936 3868 2468
Fragments arranged by size:
3868 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4937 1937
Size: 3000 4594
Acc65I G'GTAC_C
Cuts at: 3156 4915 3156
Size: 1759 5835
AclI AA'CG_TT
Cuts at: 1563 5721 6094 1563
Size: 4158 373 3063
Fragments arranged by size:
4158 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2016 2460 3392 4088 4204 4268 5657 7041
Size: 384 444 932 696 116 64 1389 1384
Cuts at: 7041 7309 1632
Size: 268 1917
Fragments arranged by size:
1917 1389 1384 932 696 444 384 268
116 64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7397
AflIII A'CryG_T
Cuts at: 1203 2508 3531 6493 7470 1203
Size: 1305 1023 2962 977 1327
Fragments arranged by size:
2962 1327 1305 1023 977
AgeI A'CCGG_T
Cuts at: 6425 6425
Size: 7594
AhdI GACnn_n'nnGTC
Cuts at: 6323 6323
Size: 7594
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3485 1184
Size: 2301 5293
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3658 3690 3658
Size: 32 7562
AlwNI CAG_nnn'CTG
Cuts at: 2878 4291 5337 6909 2878
Size: 1413 1046 1572 3563
Fragments arranged by size:
3563 1572 1413 1046
ApaI G_GGCC'C
Cuts at: 2064 4002 2064
Size: 1938 5656
ApaLI G'TGCA_C
Cuts at: 3613 5651 6807 3613
Size: 2038 1156 4400
Fragments arranged by size:
4400 2038 1156
ApoI r'AATT_y
Cuts at: 1493 4903 5129 5281 7390 1493
Size: 3410 226 152 2109 1697
Fragments arranged by size:
3410 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6148 160
Size: 872 5116 1606
Fragments arranged by size:
5116 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2243 3480 3816 4919 1870
Size: 73 96 204 1237 336 1103 4545
Fragments arranged by size:
4545 1237 1103 336 204 96 73
BamHI G'GATC_C
Cuts at: 3045 4924 5291 3045
Size: 1879 367 5348
Fragments arranged by size:
5348 1879 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 3156 4478 4678 4846
Size: 325 217 627 1136 233 1322 200 168
Cuts at: 4846 4915 6370 618
Size: 69 1455 1842
Fragments arranged by size:
1842 1455 1322 1136 627 325 233 217
200 168 69
BanII G_rGCy'C
Cuts at: 1391 2064 2240 2448 2644 3565 3817 4002 4035
Size: 673 176 208 196 921 252 185 33
Cuts at: 4035 4913 1391
Size: 878 4072
Fragments arranged by size:
4072 921 878 673 252 208 196 185
176 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2832 928
Size: 971 933 5690
Fragments arranged by size:
5690 971 933
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3461 3495 3925 3959 4230 4264 5785
Size: 34 1524 34 430 34 271 34 1521
Cuts at: 5785 5819 1903
Size: 34 3678
Fragments arranged by size:
3678 1524 1521 430 271 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5486 6696 5486
Size: 1210 6384
BclI T'GATC_A
Cuts at: 1548 2358 3187 1548
Size: 810 829 5955
Fragments arranged by size:
5955 829 810
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4286 4844 6205
Size: 107 122 71 631 605 2614 558 1361
Cuts at: 6205 136
Size: 1525
Fragments arranged by size:
2614 1525 1361 631 605 558 122 107
71
BglII A'GATC_T
Cuts at: 7589 7589
Size: 7594
BlpI GC'TnA_GC
Cuts at: 5011 5011
Size: 7594
Bme1580I G_kGCm'C
Cuts at: 1790 2064 3617 4002 4762 5655 6811 1790
Size: 274 1553 385 760 893 1156 2573
Fragments arranged by size:
2573 1553 1156 893 760 385 274
BmgBI CAC'GTC
Cuts at: 1318 3637 1318
Size: 2319 5275
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3445 5325 6283
Size: 416 53 83 399 24 2028 1880 958
Cuts at: 6283 442
Size: 1753
Fragments arranged by size:
2028 1880 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2327 2474 2900 3301 3350 3987 4313
Size: 1073 15 147 426 401 49 637 326
Cuts at: 4313 6254 1239
Size: 1941 2579
Fragments arranged by size:
2579 1941 1073 637 426 401 326 147
49 15
Bpu10I CC'TnA_GC
Cuts at: 2369 4169 2369
Size: 1800 5794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2756 5719 6584 6882 2633
Size: 123 2963 865 298 3345
Fragments arranged by size:
3345 2963 865 298 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 6257 882
Size: 617 3162 1596 2219
Fragments arranged by size:
3162 2219 1596 617
BsaAI yAC'GTr
Cuts at: 493 1204 2509 4255 4540 5385 493
Size: 711 1305 1746 285 845 2702
Fragments arranged by size:
2702 1746 1305 845 711 285
BsaBI GATnn'nnATC
Cuts at: 7588 7588
Size: 7594
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4565 5401 5783 6418
Size: 53 83 186 936 3032 836 382 635
Cuts at: 6418 275
Size: 1451
Fragments arranged by size:
3032 1451 936 836 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4302 6027 6425 6699 6846 7257 4302
Size: 1725 398 274 147 411 4639
Fragments arranged by size:
4639 1725 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2270 2300 2303 2330
Size: 30 165 30 837 30 30 3 27
Cuts at: 2330 2333 1178
Size: 3 6439
Fragments arranged by size:
6439 837 165 30 30 30 30 27
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2288 2291 2324 3304 3347 4629 4776
Size: 907 195 3 33 980 43 1282 147
Cuts at: 4776 1186
Size: 4004
Fragments arranged by size:
4004 1282 980 907 195 147 43 33
3
BseYI C'CCAG_C
Cuts at: 2057 3576 4004 4831 6797 7293 2057
Size: 1519 428 827 1966 496 2358
Fragments arranged by size:
2358 1966 1519 827 496 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3581 3797 4076 1163
Size: 573 1845 216 279 4681
Fragments arranged by size:
4681 1845 573 279 216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 5044 5805 6833 1540
Size: 275 186 18 3025 761 1028 2301
Fragments arranged by size:
3025 2301 1028 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2240 2448 2508 3565 3617 3636 3817 5655 5740
Size: 208 60 1057 52 19 181 1838 85
Cuts at: 5740 6811 2240
Size: 1071 3023
Fragments arranged by size:
3023 1838 1071 1057 208 181 85 60
52 19
BsmI GAATG_Cn'
Cuts at: 2988 3232 5105 5198 2988
Size: 244 1873 93 5384
Fragments arranged by size:
5384 1873 244 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2240 2448 2508 2644 3565 3617
Size: 399 274 176 208 60 136 921 52
Cuts at: 3617 3636 3817 4002 4035 4762 4913 5655 5740
Size: 19 181 185 33 727 151 742 85
Cuts at: 5740 6811 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 921 742 727 399 274 208
185 181 176 151 136 85 60 52
33 19
BspEI T'CCGG_A
Cuts at: 7257 7257
Size: 7594
BspHI T'CATG_A
Cuts at: 3501 5483 7213 3501
Size: 1982 1730 3882
Fragments arranged by size:
3882 1982 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4143 4949 844
Size: 3299 806 3489
Fragments arranged by size:
3489 3299 806
BsrBI CCG'CTC
Cuts at: 1394 1823 5481 1394
Size: 429 3658 3507
Fragments arranged by size:
3658 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6089 6263 65
Size: 1183 4841 174 1396
Fragments arranged by size:
4841 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3230 6238 6425 1700
Size: 110 1420 3008 187 2869
Fragments arranged by size:
3008 2869 1420 187 110
BsrGI T'GTAC_A
Cuts at: 96 3103 96
Size: 3007 4587
BssHII G'CGCG_C
Cuts at: 1925 3007 1925
Size: 1082 6512
BssSI C'ACGA_G
Cuts at: 3354 3726 4755 5654 6666 3354
Size: 372 1029 899 1012 4282
Fragments arranged by size:
4282 1029 1012 899 372
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7594
BstBI TT'CG_AA
Cuts at: 4040 6398 4040
Size: 2358 5236
BstEII G'GTnAC_C
Cuts at: 1184 4501 1184
Size: 3317 4277
BstXI CCAn_nnnn'nTGG
Cuts at: 3042 3042
Size: 7594
BstYI r'GATC_y
Cuts at: 1972 3045 4206 4924 5291 5676 5693 7145 7231
Size: 1073 1161 718 367 385 17 1452 86
Cuts at: 7231 7243 7589 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1161 1073 718 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3287 4493 4788 3287
Size: 1206 295 6093
Fragments arranged by size:
6093 1206 295
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2217 2229 3168 3657 4228
Size: 552 939 150 63 12 939 489 571
Cuts at: 4228 4809 513
Size: 581 3298
Fragments arranged by size:
3298 939 939 581 571 552 489 150
63 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2214 3921 487
Size: 683 56 988 1707 4160
Fragments arranged by size:
4160 1707 988 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 5110 5904 5924 3580
Size: 1530 794 20 5250
Fragments arranged by size:
5250 1530 794 20
ClaI AT'CG_AT
Cuts at: 1554 2955 1554
Size: 1401 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3385 3420 529
Size: 35 2821 35 4703
Fragments arranged by size:
4703 2821 35 35
DraI TTT'AAA
Cuts at: 4264 4898 4264
Size: 634 6960
DraIII CAC_nnn'GTG
Cuts at: 4685 4685
Size: 7594
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 6601 809
Size: 507 3425 1860 1802
Fragments arranged by size:
3425 1860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2133 2572
Size: 54 1425 50 275 18 186 117 439
Cuts at: 2572 3165 5041 5930 7559 8
Size: 593 1876 889 1629 43
Fragments arranged by size:
1876 1629 1425 889 593 439 275 186
117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 5041 1537
Size: 275 204 3025 4090
Fragments arranged by size:
4090 3025 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2436 3381 3912 4064 4184 4263 5524
Size: 912 171 945 531 152 120 79 1261
Cuts at: 5524 1353
Size: 3423
Fragments arranged by size:
3423 1261 945 912 531 171 152 120
79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6181 6555 6701 387
Size: 1064 138 4592 374 146 1280
Fragments arranged by size:
4592 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2238 2446 3563 3815 2238
Size: 208 1117 252 6017
Fragments arranged by size:
6017 1117 252 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 2312 2327 2460 2474 2900 3301
Size: 393 384 296 15 133 14 426 401
Cuts at: 3301 3350 3392 3987 4088 4204 4268 4313 5657
Size: 49 42 595 101 116 64 45 1344
Cuts at: 5657 6254 7041 7309 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1344 787 597 595 426 401 393
384 296 268 133 116 101 64 49
45 42 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2687 2687
Size: 7594
EcoO109I rG'GnC_Cy
Cuts at: 3998 4489 4528 3998
Size: 491 39 7064
Fragments arranged by size:
7064 491 39
EcoRI G'AATT_C
Cuts at: 4903 5281 4903
Size: 378 7216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2402 2627 3788 2402
Size: 225 1161 6208
Fragments arranged by size:
6208 1161 225
FspI TGC'GCA
Cuts at: 6100 6100
Size: 7594
HaeII r_GCGC'y
Cuts at: 1678 4771 6741 1678
Size: 3093 1970 2531
Fragments arranged by size:
3093 2531 1970
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2303 2335 2667
Size: 32 135 32 461 32 238 32 332
Cuts at: 2667 2699 3394 3426 3436 3468 6235 6267 6309
Size: 32 695 32 10 32 2767 32 42
Cuts at: 6309 6341 1373
Size: 32 2626
Fragments arranged by size:
2767 2626 695 461 332 238 135 42
32 32 32 32 32 32 32 32
32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 4938 5184 669
Size: 988 281 3000 246 3079
Fragments arranged by size:
3079 3000 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3219 748
Size: 2471 5123
HpaI GTT'AAC
Cuts at: 5184 5184
Size: 7594
KpnI G_GTAC'C
Cuts at: 3160 4919 3160
Size: 1759 5835
MfeI C'AATT_G
Cuts at: 5193 5193
Size: 7594
MluI A'CGCG_T
Cuts at: 7470 7470
Size: 7594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4234 6708 6892 1305
Size: 2929 2474 184 2007
Fragments arranged by size:
2929 2474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2574 3167 10
Size: 54 1425 343 742 593 4437
Fragments arranged by size:
4437 1425 742 593 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2511 4619 5552 5911 6070 518
Size: 648 1345 2108 933 359 159 2042
Fragments arranged by size:
2108 2042 1345 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2138 2231 2290 2878 3965 4004 4741 4811
Size: 532 93 59 588 1087 39 737 70
Cuts at: 4811 5010 5687 6835 7080 1606
Size: 199 677 1148 245 2120
Fragments arranged by size:
2120 1148 1087 737 677 588 532 245
199 93 70 59 39
NaeI GCC'GGC
Cuts at: 3232 3232
Size: 7594
NcoI C'CATG_G
Cuts at: 513 1065 2004 2154 2217 3168 3657 513
Size: 552 939 150 63 951 489 4450
Fragments arranged by size:
4450 951 939 552 489 150 63
NdeI CA'TA_TG
Cuts at: 387 2664 387
Size: 2277 5317
NgoMIV G'CCGG_C
Cuts at: 3230 3230
Size: 7594
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7594
NotI GC'GGCC_GC
Cuts at: 5041 5041
Size: 7594
NspI r_CATG'y
Cuts at: 1132 1229 3472 3535 4081 4150 4952 6497 1132
Size: 97 2243 63 546 69 802 1545 2229
Fragments arranged by size:
2243 2229 1545 802 546 97 69 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7594
PciI A'CATG_T
Cuts at: 3531 6493 3531
Size: 2962 4632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3534 1108
Size: 2426 5168
PmeI GTTT'AAAC
Cuts at: 4898 4898
Size: 7594
PmlI CAC'GTG
Cuts at: 2509 2509
Size: 7594
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5663 5695 7176 7208 5663
Size: 32 1481 32 6049
Fragments arranged by size:
6049 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4489 4489
Size: 7594
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7594
PsiI TTA'TAA
Cuts at: 5164 5164
Size: 7594
PspOMI G'GGCC_C
Cuts at: 2060 3998 2060
Size: 1938 5656
PstI C_TGCA'G
Cuts at: 830 3757 3964 4767 4783 4822 4946 830
Size: 2927 207 803 16 39 124 3478
Fragments arranged by size:
3478 2927 803 207 124 39 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7594
PvuII CAG'CTG
Cuts at: 2290 2878 3965 4741 2290
Size: 588 1087 776 5143
Fragments arranged by size:
5143 1087 776 588
SacI G_AGCT'C
Cuts at: 2240 2448 3565 3817 2240
Size: 208 1117 252 6017
Fragments arranged by size:
6017 1117 252 208
SacII CC_GC'GG
Cuts at: 2232 4812 2232
Size: 2580 5014
SalI G'TCGA_C
Cuts at: 1936 4936 1936
Size: 3000 4594
SapI GCTCTTCn'nnn_
Cuts at: 2436 3381 3912 2436
Size: 945 531 6118
Fragments arranged by size:
6118 945 531
SbfI CC_TGCA'GG
Cuts at: 4946 4946
Size: 7594
ScaI AGT'ACT
Cuts at: 2579 5842 7554 2579
Size: 3263 1712 2619
Fragments arranged by size:
3263 2619 1712
SexAI A'CCwGG_T
Cuts at: 4681 4681
Size: 7594
SfcI C'TryA_G
Cuts at: 826 1045 2870 3753 3960 4141 4763 4779 4818
Size: 219 1825 883 207 181 622 16 39
Cuts at: 4818 4942 6077 6758 6949 826
Size: 124 1135 681 191 1471
Fragments arranged by size:
1825 1471 1135 883 681 622 219 207
191 181 124 39 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7594
SmaI CCC'GGG
Cuts at: 1872 2245 4921 1872
Size: 373 2676 4545
Fragments arranged by size:
4545 2676 373
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2612 2735 5698 6599 6861 820
Size: 197 926 669 123 2963 901 262 1553
Fragments arranged by size:
2963 1553 926 901 669 262 197 123
SnaBI TAC'GTA
Cuts at: 493 5385 493
Size: 4892 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7594
SphI G_CATG'C
Cuts at: 1132 4150 4952 1132
Size: 3018 802 3774
Fragments arranged by size:
3774 3018 802
SspI AAT'ATT
Cuts at: 5 52 5518 5
Size: 47 5466 2081
Fragments arranged by size:
5466 2081 47
StuI AGG'CCT
Cuts at: 1517 4187 4425 1517
Size: 2670 238 4686
Fragments arranged by size:
4686 2670 238
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2064 2154 2217 2391 2927
Size: 552 939 48 12 90 63 174 536
Cuts at: 2927 3168 3360 3657 4083 5033 513
Size: 241 192 297 426 950 3074
Fragments arranged by size:
3074 950 939 552 536 426 297 241
192 174 90 63 48 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3590 4864 5621 5638 5791 5976 6421
Size: 1255 2010 1274 757 17 153 185 445
Cuts at: 6421 325
Size: 1498
Fragments arranged by size:
2010 1498 1274 1255 757 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 3103 4439
Size: 274 80 33 51 1443 600 526 1336
Cuts at: 4439 5840 7552 96
Size: 1401 1712 138
Fragments arranged by size:
1712 1443 1401 1336 600 526 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2240 2272 7554 7586 2240
Size: 32 5282 32 2248
Fragments arranged by size:
5282 2248 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7594
XbaI T'CTAG_A
Cuts at: 4930 4930
Size: 7594
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2582 3253 3325 2398
Size: 184 671 72 6667
Fragments arranged by size:
6667 671 184 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7594
XmaI C'CCGG_G
Cuts at: 1870 2243 4919 1870
Size: 373 2676 4545
Fragments arranged by size:
4545 2676 373
XmnI GAAnn'nnTTC
Cuts at: 2427 5723 2427
Size: 3296 4298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5402 276
Size: 53 83 186 936 3868 2468
Fragments arranged by size:
3868 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PfoI PspXI PsrI RsrII
SanDI SfiI SfoI SrfI SwaI