Restriction map for
pFN21AA0148
(Circular) (Six-base) MAPSORT of: pFN21AA0148.seq Check: 9035 from: 1 to: 6748
pFN21AA0148 6748 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 3884 4558 278
Size: 53 83 186 936 1837 511 674 2468
Fragments arranged by size:
2468 1837 936 674 511 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4091 1937
Size: 2154 4594
Acc65I G'GTAC_C
Cuts at: 3637 4069 3637
Size: 432 6316
AclI AA'CG_TT
Cuts at: 1563 2087 4047 4875 5248 1563
Size: 524 1960 828 373 3063
Fragments arranged by size:
3063 1960 828 524 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 4811 6195 6463 1632
Size: 539 2640 1384 268 1917
Fragments arranged by size:
2640 1917 1384 539 268
AflII C'TTAA_G
Cuts at: 820 1017 3742 820
Size: 197 2725 3826
Fragments arranged by size:
3826 2725 197
AflIII A'CryG_T
Cuts at: 1203 3799 5647 6624 1203
Size: 2596 1848 977 1327
Fragments arranged by size:
2596 1848 1327 977
AgeI A'CCGG_T
Cuts at: 5579 5579
Size: 6748
AhdI GACnn_n'nnGTC
Cuts at: 2254 5477 2254
Size: 3223 3525
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6748
AlwNI CAG_nnn'CTG
Cuts at: 4491 6063 4491
Size: 1572 5176
ApaLI G'TGCA_C
Cuts at: 4805 5961 4805
Size: 1156 5592
ApoI r'AATT_y
Cuts at: 1493 2318 3274 4057 4283 4435 6544 1493
Size: 825 956 783 226 152 2109 1697
Fragments arranged by size:
2109 1697 956 825 783 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5302 160
Size: 872 4270 1606
Fragments arranged by size:
4270 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
AvaI C'yCGr_G
Cuts at: 1870 1943 3097 3381 4073 1870
Size: 73 1154 284 692 4545
Fragments arranged by size:
4545 1154 692 284 73
BamHI G'GATC_C
Cuts at: 2056 4078 4445 2056
Size: 2022 367 4359
Fragments arranged by size:
4359 2022 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2130 2637 3637 4069 5524
Size: 325 217 627 343 507 1000 432 1455
Cuts at: 5524 618
Size: 1842
Fragments arranged by size:
1842 1455 1000 627 507 432 343 325
217
BanII G_rGCy'C
Cuts at: 1391 3123 3741 4067 1391
Size: 1732 618 326 4072
Fragments arranged by size:
4072 1732 618 326
BbeI G_GCGC'C
Cuts at: 2134 2134
Size: 6748
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2278 3213 3713 3923 928
Size: 971 379 935 500 210 3753
Fragments arranged by size:
3753 971 935 500 379 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4939 4973 1903
Size: 34 3002 34 3678
Fragments arranged by size:
3678 3002 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3789 4640 5850 3789
Size: 851 1210 4687
Fragments arranged by size:
4687 1210 851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6748
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5359 136
Size: 107 122 71 631 605 3687 1525
Fragments arranged by size:
3687 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2398 6743 2398
Size: 4345 2403
BlpI GC'TnA_GC
Cuts at: 2774 4165 2774
Size: 1391 5357
Bme1580I G_kGCm'C
Cuts at: 1790 2640 4809 5965 1790
Size: 850 2169 1156 2573
Fragments arranged by size:
2573 2169 1156 850
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6748
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4479 5437 442
Size: 416 53 83 399 24 3062 958 1753
Fragments arranged by size:
3062 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3248 3467 3533 5408 1239
Size: 2009 219 66 1875 2579
Fragments arranged by size:
2579 2009 1875 219 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4873 5738 6036 4873
Size: 865 298 5585
Fragments arranged by size:
5585 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 3404 5411 882
Size: 617 693 1212 2007 2219
Fragments arranged by size:
2219 2007 1212 693 617
BsaAI yAC'GTr
Cuts at: 493 1204 3802 4539 493
Size: 711 2598 737 2702
Fragments arranged by size:
2702 2598 737 711
BsaBI GATnn'nnATC
Cuts at: 6742 6742
Size: 6748
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2131 3066 3370 3881
Size: 53 83 186 936 598 935 304 511
Cuts at: 3881 4555 4937 5572 275
Size: 674 382 635 1451
Fragments arranged by size:
1451 936 935 674 635 598 511 382
304 186 83 53
BsaWI w'CCGG_w
Cuts at: 2019 2962 3413 5181 5579 5853 6000 6411 2019
Size: 943 451 1768 398 274 147 411 2356
Fragments arranged by size:
2356 1768 943 451 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3420 3450 1178
Size: 30 165 30 2017 30 4476
Fragments arranged by size:
4476 2017 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3088 1186
Size: 1902 4846
BseYI C'CCAG_C
Cuts at: 5951 6447 5951
Size: 496 6252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6175
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2949 4198 4959 5987 1540
Size: 275 186 948 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 948 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2476 3123 3741 4809 4894 5965 2476
Size: 647 618 1068 85 1071 3259
Fragments arranged by size:
3259 1071 1068 647 618 85
BsiWI C'GTAC_G
Cuts at: 3987 3987
Size: 6748
BsmI GAATG_Cn'
Cuts at: 2236 4259 4352 2236
Size: 2023 93 4632
Fragments arranged by size:
4632 2023 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2882 3361 2882
Size: 479 6269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2476 2640 3123 3741 4067 4809 4894
Size: 399 686 164 483 618 326 742 85
Cuts at: 4894 5965 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 686 618 483 399 326
164 85
BspEI T'CCGG_A
Cuts at: 2019 6411 2019
Size: 4392 2356
BspHI T'CATG_A
Cuts at: 4637 6367 4637
Size: 1730 5018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3961 4103 844
Size: 3117 142 3489
Fragments arranged by size:
3489 3117 142
BsrBI CCG'CTC
Cuts at: 1394 1823 4635 1394
Size: 429 2812 3507
Fragments arranged by size:
3507 2812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5243 5417 65
Size: 1183 3995 174 1396
Fragments arranged by size:
3995 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3243 5392 5579 1700
Size: 110 1433 2149 187 2869
Fragments arranged by size:
2869 2149 1433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6748
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6748
BssSI C'ACGA_G
Cuts at: 2660 4808 5820 2660
Size: 2148 1012 3588
Fragments arranged by size:
3588 2148 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 2804 794
Size: 1448 562 4738
Fragments arranged by size:
4738 1448 562
BstBI TT'CG_AA
Cuts at: 3278 5552 3278
Size: 2274 4474
BstEII G'GTnAC_C
Cuts at: 1184 2932 4018 1184
Size: 1748 1086 3914
Fragments arranged by size:
3914 1748 1086
BstXI CCAn_nnnn'nTGG
Cuts at: 3565 4015 3565
Size: 450 6298
BstYI r'GATC_y
Cuts at: 1972 2056 2398 4078 4445 4830 4847 6299 6385
Size: 84 342 1680 367 385 17 1452 86
Cuts at: 6385 6397 6743 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1680 1452 385 367 346 342 86
84 17 12
Bsu36I CC'TnA_GG
Cuts at: 3284 3284
Size: 6748
BtgI C'CryG_G
Cuts at: 513 1065 2168 2856 513
Size: 552 1103 688 4405
Fragments arranged by size:
4405 1103 688 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2342 3979 487
Size: 683 56 1116 1637 3256
Fragments arranged by size:
3256 1637 1116 683 56
BtsI GCAGTG_nn'
Cuts at: 2116 2180 3225 4264 5058 5078 2116
Size: 64 1045 1039 794 20 3786
Fragments arranged by size:
3786 1045 1039 794 64 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6748
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6713
DraI TTT'AAA
Cuts at: 4052 4052
Size: 6748
DraIII CAC_nnn'GTG
Cuts at: 2169 2169
Size: 6748
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5755 809
Size: 507 4439 1802
Fragments arranged by size:
4439 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3620 4195 5084
Size: 54 1425 50 275 18 1790 575 889
Cuts at: 5084 6713 8
Size: 1629 43
Fragments arranged by size:
1790 1629 1425 889 575 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4195 1537
Size: 275 2383 4090
Fragments arranged by size:
4090 2383 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3361 3459 4678 1353
Size: 2008 98 1219 3423
Fragments arranged by size:
3423 2008 1219 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5335 5709 5855 387
Size: 1064 138 3746 374 146 1280
Fragments arranged by size:
3746 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3121 3739 3121
Size: 618 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 3248 3467 3533 4811 5408 6195
Size: 393 539 1077 219 66 1278 597 787
Cuts at: 6195 6463 1239
Size: 268 1524
Fragments arranged by size:
1524 1278 1077 787 597 539 393 268
219 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2499 2499
Size: 6748
EcoO109I rG'GnC_Cy
Cuts at: 3931 3931
Size: 6748
EcoRI G'AATT_C
Cuts at: 2318 4057 4435 2318
Size: 1739 378 4631
Fragments arranged by size:
4631 1739 378
FspI TGC'GCA
Cuts at: 5254 5254
Size: 6748
HaeII r_GCGC'y
Cuts at: 1678 2134 2659 2928 5895 1678
Size: 456 525 269 2967 2531
Fragments arranged by size:
2967 2531 525 456 269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 2966 2998 3401
Size: 32 135 32 668 32 694 32 403
Cuts at: 3401 3433 3923 3955 5389 5421 5463 5495 1373
Size: 32 490 32 1434 32 42 32 2626
Fragments arranged by size:
2626 1434 694 668 490 403 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 4092 4338 669
Size: 988 281 940 1214 246 3079
Fragments arranged by size:
3079 1214 988 940 281 246
HindIII A'AGCT_T
Cuts at: 748 3251 748
Size: 2503 4245
HpaI GTT'AAC
Cuts at: 4338 4338
Size: 6748
KasI G'GCGC_C
Cuts at: 2130 2130
Size: 6748
KpnI G_GTAC'C
Cuts at: 3641 4073 3641
Size: 432 6316
MfeI C'AATT_G
Cuts at: 4347 4347
Size: 6748
MluI A'CGCG_T
Cuts at: 6624 6624
Size: 6748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2201 3372 5862 6046 1305
Size: 896 1171 2490 184 2007
Fragments arranged by size:
2490 2007 1171 896 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3622 10
Size: 54 1425 343 1790 3136
Fragments arranged by size:
3136 1790 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 2861 3401 4706 5065 5224 518
Size: 648 1564 131 540 1305 359 159 2042
Fragments arranged by size:
2042 1564 1305 648 540 359 159 131
MspA1I CmG'CkG
Cuts at: 1606 2049 3958 4015 4164 4841 5989 6234 1606
Size: 443 1909 57 149 677 1148 245 2120
Fragments arranged by size:
2120 1909 1148 677 443 245 149 57
NarI GG'CG_CC
Cuts at: 2131 2131
Size: 6748
NcoI C'CATG_G
Cuts at: 513 1065 2856 513
Size: 552 1791 4405
Fragments arranged by size:
4405 1791 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6748
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6748
NotI GC'GGCC_GC
Cuts at: 4195 4195
Size: 6748
NspI r_CATG'y
Cuts at: 1132 1229 4106 5651 1132
Size: 97 2877 1545 2229
Fragments arranged by size:
2877 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3926 1348
Size: 2578 4170
PciI A'CATG_T
Cuts at: 5647 5647
Size: 6748
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 3629 1108
Size: 1539 982 4227
Fragments arranged by size:
4227 1539 982
PmeI GTTT'AAAC
Cuts at: 4052 4052
Size: 6748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4817 4849 6330 6362 4817
Size: 32 1481 32 5203
Fragments arranged by size:
5203 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3931 3931
Size: 6748
PshAI GACnn'nnGTC
Cuts at: 1613 2945 3373 3901 1613
Size: 1332 428 528 4460
Fragments arranged by size:
4460 1332 528 428
PsiI TTA'TAA
Cuts at: 2747 4318 2747
Size: 1571 5177
PstI C_TGCA'G
Cuts at: 830 2048 2111 3903 4100 830
Size: 1218 63 1792 197 3478
Fragments arranged by size:
3478 1792 1218 197 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6748
PvuII CAG'CTG
Cuts at: 3958 4015 3958
Size: 57 6691
SacI G_AGCT'C
Cuts at: 3123 3741 3123
Size: 618 6130
SalI G'TCGA_C
Cuts at: 1936 4090 1936
Size: 2154 4594
SanDI GG'GwC_CC
Cuts at: 3931 3931
Size: 6748
SbfI CC_TGCA'GG
Cuts at: 4100 4100
Size: 6748
ScaI AGT'ACT
Cuts at: 2978 4996 6708 2978
Size: 2018 1712 3018
Fragments arranged by size:
3018 2018 1712
SfcI C'TryA_G
Cuts at: 826 1045 2044 2107 3899 4096 5231 5912 6103
Size: 219 999 63 1792 197 1135 681 191
Cuts at: 6103 826
Size: 1471
Fragments arranged by size:
1792 1471 1135 999 681 219 197 191
63
SfoI GGC'GCC
Cuts at: 2132 2132
Size: 6748
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6748
SmaI CCC'GGG
Cuts at: 1872 3383 4075 1872
Size: 1511 692 4545
Fragments arranged by size:
4545 1511 692
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4852 5753 6015 820
Size: 197 926 1799 1110 901 262 1553
Fragments arranged by size:
1799 1553 1110 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4539 493
Size: 4046 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6748
SphI G_CATG'C
Cuts at: 1132 4106 1132
Size: 2974 3774
SspI AAT'ATT
Cuts at: 5 52 4672 5
Size: 47 4620 2081
Fragments arranged by size:
4620 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6748
StyI C'CwwG_G
Cuts at: 513 1065 2856 3999 4187 513
Size: 552 1791 1143 188 3074
Fragments arranged by size:
3074 1791 1143 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3958 4775 4792 4945 5130 5575 325
Size: 1255 2378 817 17 153 185 445 1498
Fragments arranged by size:
2378 1498 1255 817 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2299 2976 3333
Size: 274 80 33 51 1443 322 677 357
Cuts at: 3333 4994 6706 96
Size: 1661 1712 138
Fragments arranged by size:
1712 1661 1443 677 357 322 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6708 6740 6708
Size: 32 6716
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6748
XbaI T'CTAG_A
Cuts at: 4084 4084
Size: 6748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3850 3850
Size: 6748
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6748
XmaI C'CCGG_G
Cuts at: 1870 3381 4073 1870
Size: 1511 692 4545
Fragments arranged by size:
4545 1511 692
XmnI GAAnn'nnTTC
Cuts at: 4877 4877
Size: 6748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 3882 4556 276
Size: 53 83 186 936 1837 511 674 2468
Fragments arranged by size:
2468 1837 936 674 511 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BstZ17I EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI