Restriction map for
pFN21AA0144
(Circular) (Six-base) MAPSORT of: pFN21AA0144.seq Check: 9445 from: 1 to: 7654
pFN21AA0144 7654 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3331 3331
Size: 7654
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5464 278
Size: 53 83 186 936 3928 2468
Fragments arranged by size:
3928 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3653 3737 4997 1937
Size: 1716 84 1260 4594
Fragments arranged by size:
4594 1716 1260 84
Acc65I G'GTAC_C
Cuts at: 3807 4975 3807
Size: 1168 6486
AclI AA'CG_TT
Cuts at: 1563 5781 6154 1563
Size: 4218 373 3063
Fragments arranged by size:
4218 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2763 2784 2973 3293 3399 3850 4338 4466
Size: 1131 21 189 320 106 451 488 128
Cuts at: 4466 5717 7101 7369 1632
Size: 1251 1384 268 1917
Fragments arranged by size:
1917 1384 1251 1131 488 451 320 268
189 128 106 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7457
AflIII A'CryG_T
Cuts at: 1203 6553 7530 1203
Size: 5350 977 1327
Fragments arranged by size:
5350 1327 977
AgeI A'CCGG_T
Cuts at: 6485 6485
Size: 7654
AhdI GACnn_n'nnGTC
Cuts at: 3424 6383 3424
Size: 2959 4695
AleI CACnn'nnGTG
Cuts at: 1166 4167 4169 1166
Size: 3001 2 4651
Fragments arranged by size:
4651 3001 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7654
AlwNI CAG_nnn'CTG
Cuts at: 2944 3457 3584 3926 4235 5397 6969 2944
Size: 513 127 342 309 1162 1572 3629
Fragments arranged by size:
3629 1572 1162 513 342 309 127
ApaI G_GGCC'C
Cuts at: 3773 3773
Size: 7654
ApaLI G'TGCA_C
Cuts at: 3245 5711 6867 3245
Size: 2466 1156 4032
Fragments arranged by size:
4032 2466 1156
ApoI r'AATT_y
Cuts at: 1493 4963 5189 5341 7450 1493
Size: 3470 226 152 2109 1697
Fragments arranged by size:
3470 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6208 160
Size: 872 5176 1606
Fragments arranged by size:
5176 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7654
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 4756 4979 1870
Size: 73 91 2722 223 4545
Fragments arranged by size:
4545 2722 223 91 73
AvrII C'CTAG_G
Cuts at: 4300 4300
Size: 7654
BamHI G'GATC_C
Cuts at: 2911 4984 5351 2911
Size: 2073 367 5214
Fragments arranged by size:
5214 2073 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2432 2480 3807 4975 6430
Size: 325 217 627 645 48 1327 1168 1455
Cuts at: 6430 618
Size: 1842
Fragments arranged by size:
1842 1455 1327 1168 645 627 325 217
48
BanII G_rGCy'C
Cuts at: 1391 2035 3185 3773 4973 1391
Size: 644 1150 588 1200 4072
Fragments arranged by size:
4072 1200 1150 644 588
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2790 2879 3354 928
Size: 971 891 89 475 5228
Fragments arranged by size:
5228 971 891 475 89
BbvCI CC'TCA_GC
Cuts at: 3438 4295 3438
Size: 857 6797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5845 5879 1903
Size: 34 3908 34 3678
Fragments arranged by size:
3908 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4889 5546 6756 4889
Size: 657 1210 5787
Fragments arranged by size:
5787 1210 657
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7654
BfrBI ATG'CAT
Cuts at: 4850 4850
Size: 7654
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6265 136
Size: 107 122 71 631 605 4593 1525
Fragments arranged by size:
4593 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2758 2775 7649 2758
Size: 17 4874 2763
Fragments arranged by size:
4874 2763 17
BlpI GC'TnA_GC
Cuts at: 3135 3447 3526 5071 3135
Size: 312 79 1545 5718
Fragments arranged by size:
5718 1545 312 79
Bme1580I G_kGCm'C
Cuts at: 1790 2024 3249 3773 5715 6871 1790
Size: 234 1225 524 1942 1156 2573
Fragments arranged by size:
2573 1942 1225 1156 524 234
BmgBI CAC'GTC
Cuts at: 1318 2405 1318
Size: 1087 6567
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 3079 4241
Size: 416 53 83 399 24 636 1026 1162
Cuts at: 4241 4554 5385 6343 442
Size: 313 831 958 1753
Fragments arranged by size:
1753 1162 1026 958 831 636 416 399
313 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4186 4249 4407 6314 1239
Size: 2947 63 158 1907 2579
Fragments arranged by size:
2947 2579 1907 158 63
Bpu10I CC'TnA_GC
Cuts at: 3438 3765 4295 3438
Size: 327 530 6797
Fragments arranged by size:
6797 530 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2568 4060 5779 6644 6942 2568
Size: 1492 1719 865 298 3280
Fragments arranged by size:
3280 1719 1492 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2370 2762 4893 6317 882
Size: 617 871 392 2131 1424 2219
Fragments arranged by size:
2219 2131 1424 871 617 392
BsaAI yAC'GTr
Cuts at: 493 1204 2667 5445 493
Size: 711 1463 2778 2702
Fragments arranged by size:
2778 2702 1463 711
BsaBI GATnn'nnATC
Cuts at: 7648 7648
Size: 7654
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5461 5843 6478 275
Size: 53 83 186 936 3928 382 635 1451
Fragments arranged by size:
3928 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6087 6485 6759 6906 7317 6087
Size: 398 274 147 411 6424
Fragments arranged by size:
6424 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3376 3406 3800 3830 4540
Size: 30 165 30 1973 30 394 30 710
Cuts at: 4540 4570 1178
Size: 30 4262
Fragments arranged by size:
4262 1973 710 394 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2580 2744 3157 3357 4067 4252 4356
Size: 985 409 164 413 200 710 185 104
Cuts at: 4356 4606 1186
Size: 250 4234
Fragments arranged by size:
4234 985 710 413 409 250 200 185
164 104
BseYI C'CCAG_C
Cuts at: 2605 2866 2937 3812 4621 4766 6857 7353 2605
Size: 261 71 875 809 145 2091 496 2906
Fragments arranged by size:
2906 2091 875 809 496 261 145 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3230 3330 3896 4221 1163
Size: 573 1494 100 566 325 4596
Fragments arranged by size:
4596 1494 573 566 325 100
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4603 5104 5865 6893 1540
Size: 275 186 2602 501 761 1028 2301
Fragments arranged by size:
2602 2301 1028 761 501 275 186
BsiHKAI G_wGCw'C
Cuts at: 3249 5715 5800 6871 3249
Size: 2466 85 1071 4032
Fragments arranged by size:
4032 2466 1071 85
BsmI GAATG_Cn'
Cuts at: 2284 3139 4018 4852 5165 5258 2284
Size: 855 879 834 313 93 4680
Fragments arranged by size:
4680 879 855 834 313 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2922 2922
Size: 7654
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2035 3185 3249 3773 4973 5715
Size: 399 234 11 1150 64 524 1200 742
Cuts at: 5715 5800 6871 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1200 1150 1071 742 524 399 234
85 64 11
BspEI T'CCGG_A
Cuts at: 7317 7317
Size: 7654
BspHI T'CATG_A
Cuts at: 5543 7273 5543
Size: 1730 5924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3331 3558 5009 844
Size: 2487 227 1451 3489
Fragments arranged by size:
3489 2487 1451 227
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 5541 1394
Size: 429 1900 1818 3507
Fragments arranged by size:
3507 1900 1818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2236 3563 6149 6323 65
Size: 1183 988 1327 2586 174 1396
Fragments arranged by size:
2586 1396 1327 1183 988 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3442 6298 6485 1700
Size: 110 1632 2856 187 2869
Fragments arranged by size:
2869 2856 1632 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7654
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7654
BssSI C'ACGA_G
Cuts at: 3616 5714 6726 3616
Size: 2098 1012 4544
Fragments arranged by size:
4544 2098 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7654
BstBI TT'CG_AA
Cuts at: 2960 6458 2960
Size: 3498 4156
BstEII G'GTnAC_C
Cuts at: 1184 4583 1184
Size: 3399 4255
BstXI CCAn_nnnn'nTGG
Cuts at: 2333 4639 2333
Size: 2306 5348
BstYI r'GATC_y
Cuts at: 1972 2758 2775 2911 3410 3788 4984 5351 5736
Size: 786 17 136 499 378 1196 367 385
Cuts at: 5736 5753 7205 7291 7303 7649 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1196 786 499 385 378 367
346 136 86 17 17 12
BstZ17I GTA'TAC
Cuts at: 3654 3654
Size: 7654
BtgI C'CryG_G
Cuts at: 513 1065 2440 2533 4603 513
Size: 552 1375 93 2070 3564
Fragments arranged by size:
3564 2070 1375 552 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6915
Fragments arranged by size:
6915 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2781 2832 3249 3333 3936 5170 5964 5984
Size: 684 51 417 84 603 1234 794 20
Cuts at: 5984 2097
Size: 3767
Fragments arranged by size:
3767 1234 794 684 603 417 84 51
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7654
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7619
DraI TTT'AAA
Cuts at: 4958 4958
Size: 7654
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6661 809
Size: 507 5345 1802
Fragments arranged by size:
5345 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2087 2338 2536
Size: 54 1425 50 275 18 257 251 198
Cuts at: 2536 2680 3663 4563 4600 4629 4854 5101 5990
Size: 144 983 900 37 29 225 247 889
Cuts at: 5990 7619 8
Size: 1629 43
Fragments arranged by size:
1629 1425 983 900 889 275 257 251
247 225 198 144 54 50 43 37
29 18
EagI C'GGCC_G
Cuts at: 1537 1812 4600 5101 1537
Size: 275 2788 501 4090
Fragments arranged by size:
4090 2788 501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2500 3631 3829 4819 5584 1353
Size: 1147 1131 198 990 765 3423
Fragments arranged by size:
3423 1147 1131 990 765 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6241 6615 6761 387
Size: 1064 138 4652 374 146 1280
Fragments arranged by size:
4652 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2763 2784 2973 3293 3399 3850 4186
Size: 393 1131 21 189 320 106 451 336
Cuts at: 4186 4249 4338 4407 4466 5717 6314 7101 7369
Size: 63 89 69 59 1251 597 787 268
Cuts at: 7369 1239
Size: 1524
Fragments arranged by size:
1524 1251 1131 787 597 451 393 336
320 268 189 106 89 69 63 59
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2988 3848 2988
Size: 860 6794
EcoO109I rG'GnC_Cy
Cuts at: 2495 3769 3770 3841 4387 4753 2495
Size: 1274 1 71 546 366 5396
Fragments arranged by size:
5396 1274 546 366 71 1
EcoRI G'AATT_C
Cuts at: 4963 5341 4963
Size: 378 7276
EcoRV GAT'ATC
Cuts at: 3535 3535
Size: 7654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3880 3880
Size: 7654
FspI TGC'GCA
Cuts at: 6160 6160
Size: 7654
HaeII r_GCGC'y
Cuts at: 1678 3268 3624 4709 6801 1678
Size: 1590 356 1085 2092 2531
Fragments arranged by size:
2531 2092 1590 1085 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3167 3199 3410 3442 3487
Size: 32 135 32 1595 32 211 32 45
Cuts at: 3487 3519 3587 3619 3997 4029 4138 4170 4601
Size: 32 68 32 378 32 109 32 431
Cuts at: 4601 4633 4654 4686 6295 6327 6369 6401 1373
Size: 32 21 32 1609 32 42 32 2626
Fragments arranged by size:
2626 1609 1595 431 378 211 135 109
68 45 42 32 32 32 32 32
32 32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 3738 4998 5244 669
Size: 988 281 304 1496 1260 246 3079
Fragments arranged by size:
3079 1496 1260 988 304 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7654
HpaI GTT'AAC
Cuts at: 5244 5244
Size: 7654
KpnI G_GTAC'C
Cuts at: 3811 4979 3811
Size: 1168 6486
MfeI C'AATT_G
Cuts at: 2171 5253 2171
Size: 3082 4572
MluI A'CGCG_T
Cuts at: 7530 7530
Size: 7654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4891 6768 6952 1305
Size: 3586 1877 184 2007
Fragments arranged by size:
3586 2007 1877 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2340 2682 4565 10
Size: 54 1425 343 508 342 1883 3099
Fragments arranged by size:
3099 1883 1425 508 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 4167 4169 5612 5971 6130 518
Size: 648 976 2025 2 1443 359 159 2042
Fragments arranged by size:
2042 2025 1443 976 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2086 2609 2658 3344 4540 5070 5747 6895
Size: 480 523 49 686 1196 530 677 1148
Cuts at: 6895 7140 1606
Size: 245 2120
Fragments arranged by size:
2120 1196 1148 686 677 530 523 480
245 49
NaeI GCC'GGC
Cuts at: 3444 3444
Size: 7654
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7654
NgoMIV G'CCGG_C
Cuts at: 3442 3442
Size: 7654
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7654
NotI GC'GGCC_GC
Cuts at: 5101 5101
Size: 7654
NsiI A_TGCA'T
Cuts at: 4852 4852
Size: 7654
NspI r_CATG'y
Cuts at: 1132 1229 3028 4439 5012 6557 1132
Size: 97 1799 1411 573 1545 2229
Fragments arranged by size:
2229 1799 1545 1411 573 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7654
PciI A'CATG_T
Cuts at: 6553 6553
Size: 7654
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2699 2944 3091 3118 1108
Size: 1591 245 147 27 5644
Fragments arranged by size:
5644 1591 245 147 27
PmeI GTTT'AAAC
Cuts at: 4958 4958
Size: 7654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5723 5755 7236 7268 5723
Size: 32 1481 32 6109
Fragments arranged by size:
6109 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3841 4387 4753 3841
Size: 546 366 6742
Fragments arranged by size:
6742 546 366
PshAI GACnn'nnGTC
Cuts at: 1613 2394 1613
Size: 781 6873
PsiI TTA'TAA
Cuts at: 5224 5224
Size: 7654
PspOMI G'GGCC_C
Cuts at: 3769 3769
Size: 7654
PstI C_TGCA'G
Cuts at: 830 2104 3328 3555 3931 3991 5006 830
Size: 1274 1224 227 376 60 1015 3478
Fragments arranged by size:
3478 1274 1224 1015 376 227 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7654
PvuII CAG'CTG
Cuts at: 2609 3344 2609
Size: 735 6919
SalI G'TCGA_C
Cuts at: 1936 3736 4996 1936
Size: 1800 1260 4594
Fragments arranged by size:
4594 1800 1260
SanDI GG'GwC_CC
Cuts at: 4387 4753 4387
Size: 366 7288
SbfI CC_TGCA'GG
Cuts at: 5006 5006
Size: 7654
ScaI AGT'ACT
Cuts at: 3073 5902 7614 3073
Size: 2829 1712 3113
Fragments arranged by size:
3113 2829 1712
SfcI C'TryA_G
Cuts at: 826 1045 2100 2392 3075 3324 3551 3918 3927
Size: 219 1055 292 683 249 227 367 9
Cuts at: 3927 3987 4507 4720 5002 6137 6818 7009 826
Size: 60 520 213 282 1135 681 191 1471
Fragments arranged by size:
1471 1135 1055 683 681 520 367 292
282 249 227 219 213 191 60 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7654
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7654
SmaI CCC'GGG
Cuts at: 1872 2036 4758 4981 1872
Size: 164 2722 223 4545
Fragments arranged by size:
4545 2722 223 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2583 4039 5758 6659 6921 820
Size: 197 926 640 1456 1719 901 262 1553
Fragments arranged by size:
1719 1553 1456 926 901 640 262 197
SnaBI TAC'GTA
Cuts at: 493 5445 493
Size: 4952 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7654
SphI G_CATG'C
Cuts at: 1132 5012 1132
Size: 3880 3774
SspI AAT'ATT
Cuts at: 5 52 5578 5
Size: 47 5526 2081
Fragments arranged by size:
5526 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7654
StyI C'CwwG_G
Cuts at: 513 1065 4029 4300 4638 5093 513
Size: 552 2964 271 338 455 3074
Fragments arranged by size:
3074 2964 552 455 338 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4022 4543 5681 5698 5851 6036 6481
Size: 1255 2442 521 1138 17 153 185 445
Cuts at: 6481 325
Size: 1498
Fragments arranged by size:
2442 1498 1255 1138 521 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3071 5900 7612
Size: 274 80 33 51 1443 1094 2829 1712
Cuts at: 7612 96
Size: 138
Fragments arranged by size:
2829 1712 1443 1094 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3376 3408 7614 7646 3376
Size: 32 4206 32 3384
Fragments arranged by size:
4206 3384 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7654
XbaI T'CTAG_A
Cuts at: 4990 4990
Size: 7654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 4874 2333
Size: 2541 5113
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7654
XmaI C'CCGG_G
Cuts at: 1870 2034 4756 4979 1870
Size: 164 2722 223 4545
Fragments arranged by size:
4545 2722 223 164
XmnI GAAnn'nnTTC
Cuts at: 2575 3863 5783 2575
Size: 1288 1920 4446
Fragments arranged by size:
4446 1920 1288
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5462 276
Size: 53 83 186 936 3928 2468
Fragments arranged by size:
3928 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BplI BsiWI Bsu36I
DraIII EcoICRI FseI FspAI KasI NarI NruI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI