Restriction map for
pFN21AA0143
(Circular) (Six-base) MAPSORT of: pFN21AA0143.seq Check: 4983 from: 1 to: 7168
pFN21AA0143 7168 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4978 278
Size: 53 83 186 936 3442 2468
Fragments arranged by size:
3442 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4511 1937
Size: 2574 4594
Acc65I G'GTAC_C
Cuts at: 4489 4489
Size: 7168
AclI AA'CG_TT
Cuts at: 1563 3633 5295 5668 1563
Size: 2070 1662 373 3063
Fragments arranged by size:
3063 2070 1662 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2100 3910 4194 5231 6615 6883 1632
Size: 468 1810 284 1037 1384 268 1917
Fragments arranged by size:
1917 1810 1384 1037 468 284 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6971
AflIII A'CryG_T
Cuts at: 1203 6067 7044 1203
Size: 4864 977 1327
Fragments arranged by size:
4864 1327 977
AgeI A'CCGG_T
Cuts at: 5999 5999
Size: 7168
AhdI GACnn_n'nnGTC
Cuts at: 3437 5897 3437
Size: 2460 4708
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2845 1184
Size: 1661 5507
AlwNI CAG_nnn'CTG
Cuts at: 2141 2956 4911 6483 2141
Size: 815 1955 1572 2826
Fragments arranged by size:
2826 1955 1572 815
ApaLI G'TGCA_C
Cuts at: 5225 6381 5225
Size: 1156 6012
ApoI r'AATT_y
Cuts at: 1493 2365 2378 2816 3053 3297 3546 3882 4265
Size: 872 13 438 237 244 249 336 383
Cuts at: 4265 4318 4477 4703 4855 6964 1493
Size: 53 159 226 152 2109 1697
Fragments arranged by size:
2109 1697 872 438 383 336 249 244
237 226 159 152 53 13
AseI AT'TA_AT
Cuts at: 160 1032 5722 160
Size: 872 4690 1606
Fragments arranged by size:
4690 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7168
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2928 4493 1870
Size: 73 70 915 1565 4545
Fragments arranged by size:
4545 1565 915 73 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3578 3611 3578
Size: 33 7135
BamHI G'GATC_C
Cuts at: 2806 3388 4498 4865 2806
Size: 582 1110 367 5109
Fragments arranged by size:
5109 1110 582 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4489 5944 618
Size: 325 217 627 2702 1455 1842
Fragments arranged by size:
2702 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4487 1391
Size: 3096 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 3007 3359 3633 928
Size: 971 504 604 352 274 4463
Fragments arranged by size:
4463 971 604 504 352 274
BbvCI CC'TCA_GC
Cuts at: 2577 2577
Size: 7168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2004 2021 2038 5359 5393 1903
Size: 34 50 17 17 17 3321 34 3678
Fragments arranged by size:
3678 3321 50 34 34 17 17 17
BciVI GTATCCnnnnn_n'
Cuts at: 4123 5060 6270 4123
Size: 937 1210 5021
Fragments arranged by size:
5021 1210 937
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7168
BfrBI ATG'CAT
Cuts at: 3454 3454
Size: 7168
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5779 136
Size: 107 122 71 631 605 4107 1525
Fragments arranged by size:
4107 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3317 4453 7163 3317
Size: 1136 2710 3322
Fragments arranged by size:
3322 2710 1136
BlpI GC'TnA_GC
Cuts at: 4585 4585
Size: 7168
Bme1580I G_kGCm'C
Cuts at: 1790 5229 6385 1790
Size: 3439 1156 2573
Fragments arranged by size:
3439 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4368 4424 4899
Size: 416 53 83 399 24 2951 56 475
Cuts at: 4899 5857 442
Size: 958 1753
Fragments arranged by size:
2951 1753 958 475 416 399 83 56
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 5828 1239
Size: 891 3698 2579
Fragments arranged by size:
3698 2579 891
Bpu10I CC'TnA_GC
Cuts at: 2577 2577
Size: 7168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5293 6158 6456 5293
Size: 865 298 6005
Fragments arranged by size:
6005 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5831 882
Size: 617 4332 2219
Fragments arranged by size:
4332 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4959 493
Size: 711 3755 2702
Fragments arranged by size:
3755 2702 711
BsaBI GATnn'nnATC
Cuts at: 4074 7162 4074
Size: 3088 4080
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4249 4975 5357 5992
Size: 53 83 186 936 2716 726 382 635
Cuts at: 5992 275
Size: 1451
Fragments arranged by size:
2716 1451 936 726 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3511 5601 5999 6273 6420 6831 3511
Size: 2090 398 274 147 411 3848
Fragments arranged by size:
3848 2090 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6943
Fragments arranged by size:
6943 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4359 1186
Size: 3173 3995
BseYI C'CCAG_C
Cuts at: 2728 6371 6867 2728
Size: 3643 496 3029
Fragments arranged by size:
3643 3029 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3158 3351 1163
Size: 573 1422 193 4980
Fragments arranged by size:
4980 1422 573 193
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4618 5379 6407 1540
Size: 275 186 2617 761 1028 2301
Fragments arranged by size:
2617 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5229 5314 6385 5229
Size: 85 1071 6012
Fragments arranged by size:
6012 1071 85
BsmI GAATG_Cn'
Cuts at: 3148 4679 4772 3148
Size: 1531 93 5544
Fragments arranged by size:
5544 1531 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3047 3047
Size: 7168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4487 5229 5314 6385 1391
Size: 399 2697 742 85 1071 2174
Fragments arranged by size:
2697 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6831 6831
Size: 7168
BspHI T'CATG_A
Cuts at: 3207 3472 3570 4327 5057 6787 3207
Size: 265 98 757 730 1730 3588
Fragments arranged by size:
3588 1730 757 730 265 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 4523 844
Size: 2086 1593 3489
Fragments arranged by size:
3489 2086 1593
BsrBI CCG'CTC
Cuts at: 1394 1823 5055 1394
Size: 429 3232 3507
Fragments arranged by size:
3507 3232 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2973 3729 5663 5837 65
Size: 1183 1725 756 1934 174 1396
Fragments arranged by size:
1934 1725 1396 1183 756 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5812 5999 1700
Size: 110 4002 187 2869
Fragments arranged by size:
4002 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7168
BssSI C'ACGA_G
Cuts at: 5228 6240 5228
Size: 1012 6156
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2141 3806 794
Size: 1347 1665 4156
Fragments arranged by size:
4156 1665 1347
BstBI TT'CG_AA
Cuts at: 3057 3886 5972 3057
Size: 829 2086 4253
Fragments arranged by size:
4253 2086 829
BstEII G'GTnAC_C
Cuts at: 1184 3331 1184
Size: 2147 5021
BstXI CCAn_nnnn'nTGG
Cuts at: 3788 4123 4222 3788
Size: 335 99 6734
Fragments arranged by size:
6734 335 99
BstYI r'GATC_y
Cuts at: 1972 2083 2806 3080 3317 3388 4453 4498 4865
Size: 111 723 274 237 71 1065 45 367
Cuts at: 4865 5250 5267 6719 6805 6817 7163 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1065 723 385 367 346 274
237 111 86 71 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6616
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6429
Fragments arranged by size:
6429 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3097 3368 4684 5478 5498 2827
Size: 270 271 1316 794 20 4497
Fragments arranged by size:
4497 1316 794 271 270 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7133
DraI TTT'AAA
Cuts at: 2836 4472 2836
Size: 1636 5532
DraIII CAC_nnn'GTG
Cuts at: 2878 4304 2878
Size: 1426 5742
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6175 809
Size: 507 4859 1802
Fragments arranged by size:
4859 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3726 4615 5504
Size: 54 1425 50 275 18 1896 889 889
Cuts at: 5504 7133 8
Size: 1629 43
Fragments arranged by size:
1896 1629 1425 889 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4615 1537
Size: 275 2803 4090
Fragments arranged by size:
4090 2803 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2679 5098 1353
Size: 1326 2419 3423
Fragments arranged by size:
3423 2419 1326
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5755 6129 6275 387
Size: 1064 138 4166 374 146 1280
Fragments arranged by size:
4166 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2130 3910 4194 5231 5828 6615
Size: 393 468 30 1780 284 1037 597 787
Cuts at: 6615 6883 1239
Size: 268 1524
Fragments arranged by size:
1780 1524 1037 787 597 468 393 284
268 30
EcoO109I rG'GnC_Cy
Cuts at: 2651 2651
Size: 7168
EcoRI G'AATT_C
Cuts at: 3053 3297 4477 4855 3053
Size: 244 1180 378 5366
Fragments arranged by size:
5366 1180 378 244
EcoRV GAT'ATC
Cuts at: 3265 3265
Size: 7168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2114 4097 2114
Size: 1983 5185
FspI TGC'GCA
Cuts at: 2781 5674 2781
Size: 2893 4275
HaeII r_GCGC'y
Cuts at: 1678 6315 1678
Size: 4637 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 3423 3455 4025
Size: 32 135 32 818 32 1001 32 570
Cuts at: 4025 4057 4097 4118 4129 4150 5809 5841 5883
Size: 32 40 21 11 21 1659 32 42
Cuts at: 5883 5915 1373
Size: 32 2626
Fragments arranged by size:
2626 1659 1001 818 570 135 42 40
32 32 32 32 32 32 32 21
21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2545 2638 3097 3272 4512 4758
Size: 988 281 607 93 459 175 1240 246
Cuts at: 4758 669
Size: 3079
Fragments arranged by size:
3079 1240 988 607 459 281 246 175
93
HindIII A'AGCT_T
Cuts at: 748 2299 2345 3488 748
Size: 1551 46 1143 4428
Fragments arranged by size:
4428 1551 1143 46
HpaI GTT'AAC
Cuts at: 4758 4758
Size: 7168
KpnI G_GTAC'C
Cuts at: 4493 4493
Size: 7168
MfeI C'AATT_G
Cuts at: 4206 4767 4206
Size: 561 6607
MluI A'CGCG_T
Cuts at: 7044 7044
Size: 7168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3023 4161 6282 6466 1305
Size: 1718 1138 2121 184 2007
Fragments arranged by size:
2121 2007 1718 1138 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3728 10
Size: 54 1425 343 1896 3450
Fragments arranged by size:
3450 1896 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2904 3786 4121 5126 5485 5644 518
Size: 648 1738 882 335 1005 359 159 2042
Fragments arranged by size:
2042 1738 1005 882 648 359 335 159
MspA1I CmG'CkG
Cuts at: 1606 3592 4584 5261 6409 6654 1606
Size: 1986 992 677 1148 245 2120
Fragments arranged by size:
2120 1986 1148 992 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6616
NdeI CA'TA_TG
Cuts at: 387 2310 2583 387
Size: 1923 273 4972
Fragments arranged by size:
4972 1923 273
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7168
NotI GC'GGCC_GC
Cuts at: 4615 4615
Size: 7168
NsiI A_TGCA'T
Cuts at: 3456 3456
Size: 7168
NspI r_CATG'y
Cuts at: 1132 1229 2620 3866 3943 4412 4526 6071 1132
Size: 97 1391 1246 77 469 114 1545 2229
Fragments arranged by size:
2229 1545 1391 1246 469 114 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7168
PciI A'CATG_T
Cuts at: 6067 6067
Size: 7168
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2093 2568 3259 4348 1108
Size: 985 475 691 1089 3928
Fragments arranged by size:
3928 1089 985 691 475
PfoI T'CCnGG_A
Cuts at: 2883 3384 2883
Size: 501 6667
PmeI GTTT'AAAC
Cuts at: 4472 4472
Size: 7168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5237 5269 6750 6782 5237
Size: 32 1481 32 5623
Fragments arranged by size:
5623 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7168
PsiI TTA'TAA
Cuts at: 2843 3677 4738 2843
Size: 834 1061 5273
Fragments arranged by size:
5273 1061 834
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4344 4376 4344
Size: 32 7136
PstI C_TGCA'G
Cuts at: 830 4520 830
Size: 3690 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7168
PvuII CAG'CTG
Cuts at: 3592 3592
Size: 7168
SalI G'TCGA_C
Cuts at: 1936 4510 1936
Size: 2574 4594
SbfI CC_TGCA'GG
Cuts at: 4520 4520
Size: 7168
ScaI AGT'ACT
Cuts at: 5416 7128 5416
Size: 1712 5456
SfcI C'TryA_G
Cuts at: 826 1045 3084 4516 5651 6332 6523 826
Size: 219 2039 1432 1135 681 191 1471
Fragments arranged by size:
2039 1471 1432 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7168
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7168
SmaI CCC'GGG
Cuts at: 1872 2930 4495 1872
Size: 1058 1565 4545
Fragments arranged by size:
4545 1565 1058
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5272 6173 6435 820
Size: 197 926 3329 901 262 1553
Fragments arranged by size:
3329 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4959 493
Size: 4466 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7168
SphI G_CATG'C
Cuts at: 1132 3943 4412 4526 1132
Size: 2811 469 114 3774
Fragments arranged by size:
3774 2811 469 114
SspI AAT'ATT
Cuts at: 5 52 2389 4347 5092 5
Size: 47 2337 1958 745 2081
Fragments arranged by size:
2337 2081 1958 745 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7168
StyI C'CwwG_G
Cuts at: 513 1065 4607 513
Size: 552 3542 3074
Fragments arranged by size:
3542 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5195 5212 5365 5550 5995 325
Size: 1255 3615 17 153 185 445 1498
Fragments arranged by size:
3615 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3978 5414 7126
Size: 274 80 33 51 1443 2001 1436 1712
Cuts at: 7126 96
Size: 138
Fragments arranged by size:
2001 1712 1443 1436 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4348 4380 7128 7160 4348
Size: 32 2748 32 4356
Fragments arranged by size:
4356 2748 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7168
XbaI T'CTAG_A
Cuts at: 4085 4504 4085
Size: 419 6749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7168
XmaI C'CCGG_G
Cuts at: 1870 2928 4493 1870
Size: 1058 1565 4545
Fragments arranged by size:
4545 1565 1058
XmnI GAAnn'nnTTC
Cuts at: 2167 2301 2365 5297 2167
Size: 134 64 2932 4038
Fragments arranged by size:
4038 2932 134 64
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4976 276
Size: 53 83 186 936 3442 2468
Fragments arranged by size:
3442 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI