Restriction map for
pFN21AA0138
(Circular) (Six-base) MAPSORT of: pFN21AA0138.seq Check: 4290 from: 1 to: 7564
pFN21AA0138 7564 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5374 278
Size: 53 83 186 936 3838 2468
Fragments arranged by size:
3838 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3371 3528 4907 1937
Size: 1434 157 1379 4594
Fragments arranged by size:
4594 1434 1379 157
Acc65I G'GTAC_C
Cuts at: 4885 4885
Size: 7564
AclI AA'CG_TT
Cuts at: 1563 5691 6064 1563
Size: 4128 373 3063
Fragments arranged by size:
4128 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2150 2303 2436 3636 5627 7011 7279 1632
Size: 518 153 133 1200 1991 1384 268 1917
Fragments arranged by size:
1991 1917 1384 1200 518 268 153 133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7367
AflIII A'CryG_T
Cuts at: 1203 6463 7440 1203
Size: 5260 977 1327
Fragments arranged by size:
5260 1327 977
AgeI A'CCGG_T
Cuts at: 6395 6395
Size: 7564
AhdI GACnn_n'nnGTC
Cuts at: 3838 6293 3838
Size: 2455 5109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2739 2982 1184
Size: 1555 243 5766
Fragments arranged by size:
5766 1555 243
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3885 3917 3885
Size: 32 7532
AlwNI CAG_nnn'CTG
Cuts at: 4033 5307 6879 4033
Size: 1274 1572 4718
Fragments arranged by size:
4718 1572 1274
ApaI G_GGCC'C
Cuts at: 2042 4668 2042
Size: 2626 4938
ApaLI G'TGCA_C
Cuts at: 5621 6777 5621
Size: 1156 6408
ApoI r'AATT_y
Cuts at: 1493 4873 5099 5251 7360 1493
Size: 3380 226 152 2109 1697
Fragments arranged by size:
3380 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6118 160
Size: 872 5086 1606
Fragments arranged by size:
5086 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7564
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2035 2062 3337 3956 4013 4184
Size: 73 78 14 27 1275 619 57 171
Cuts at: 4184 4669 4889 1870
Size: 485 220 4545
Fragments arranged by size:
4545 1275 619 485 220 171 78 73
57 27 14
BamHI G'GATC_C
Cuts at: 4894 5261 4894
Size: 367 7197
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4143 4595 4753 4885 6340
Size: 325 217 627 2356 452 158 132 1455
Cuts at: 6340 618
Size: 1842
Fragments arranged by size:
2356 1842 1455 627 452 325 217 158
132
BanII G_rGCy'C
Cuts at: 1391 2042 2892 2982 3006 3152 3346 3747 4668
Size: 651 850 90 24 146 194 401 921
Cuts at: 4668 4883 1391
Size: 215 4072
Fragments arranged by size:
4072 921 850 651 401 215 194 146
90 24
BbeI G_GCGC'C
Cuts at: 4147 4147
Size: 7564
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 3063 3624 928
Size: 971 429 735 561 4868
Fragments arranged by size:
4868 971 735 561 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5755 5789 1903
Size: 34 3818 34 3678
Fragments arranged by size:
3818 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5456 6666 5456
Size: 1210 6354
BclI T'GATC_A
Cuts at: 1548 3136 1548
Size: 1588 5976
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2938 4672 6175
Size: 107 122 71 631 605 1266 1734 1503
Cuts at: 6175 136
Size: 1525
Fragments arranged by size:
1734 1525 1503 1266 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7559 7559
Size: 7564
BlpI GC'TnA_GC
Cuts at: 2093 2529 4981 2093
Size: 436 2452 4676
Fragments arranged by size:
4676 2452 436
Bme1580I G_kGCm'C
Cuts at: 1790 2042 4668 4683 5625 6781 1790
Size: 252 2626 15 942 1156 2573
Fragments arranged by size:
2626 2573 1156 942 252 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7564
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2088 4552 5295
Size: 416 53 83 399 24 671 2464 743
Cuts at: 5295 6253 442
Size: 958 1753
Fragments arranged by size:
2464 1753 958 743 671 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4620 1056
Size: 3564 4000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3805 3805
Size: 7564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2421 2732 4055 4085 6224 1239
Size: 1145 37 311 1323 30 2139 2579
Fragments arranged by size:
2579 2139 1323 1145 311 37 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3257 5689 6554 6852 2168
Size: 1089 2432 865 298 2880
Fragments arranged by size:
2880 2432 1089 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4311 4374 6227 882
Size: 617 2812 63 1853 2219
Fragments arranged by size:
2812 2219 1853 617 63
BsaAI yAC'GTr
Cuts at: 493 1204 2500 4750 5355 493
Size: 711 1296 2250 605 2702
Fragments arranged by size:
2702 2250 1296 711 605
BsaBI GATnn'nnATC
Cuts at: 7558 7558
Size: 7564
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4144 5371 5753 6388
Size: 53 83 186 936 2611 1227 382 635
Cuts at: 6388 275
Size: 1451
Fragments arranged by size:
2611 1451 1227 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2608 2797 5997 6395 6669 6816 7227 2608
Size: 189 3200 398 274 147 411 2945
Fragments arranged by size:
3200 2945 411 398 274 189 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3732 3762 1178
Size: 30 165 30 2329 30 4980
Fragments arranged by size:
4980 2329 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2306 3014 4148 4583 1186
Size: 1120 708 1134 435 4167
Fragments arranged by size:
4167 1134 1120 708 435
BseYI C'CCAG_C
Cuts at: 3467 4467 6767 7263 3467
Size: 1000 2300 496 3768
Fragments arranged by size:
3768 2300 1000 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2490 4290 1163
Size: 573 754 1800 4437
Fragments arranged by size:
4437 1800 754 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3720 4450 5014 5775 6803 1540
Size: 275 186 1719 730 564 761 1028 2301
Fragments arranged by size:
2301 1719 1028 761 730 564 275 186
BsiHKAI G_wGCw'C
Cuts at: 2437 2982 3006 3152 4554 4575 4629 5625 5710
Size: 545 24 146 1402 21 54 996 85
Cuts at: 5710 6781 2437
Size: 1071 3220
Fragments arranged by size:
3220 1402 1071 996 545 146 85 54
24 21
BsmI GAATG_Cn'
Cuts at: 5075 5168 5075
Size: 93 7471
BsmBI CGTCTCn'nnnn_
Cuts at: 2328 2328
Size: 7564
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2437 2892 2982 3006 3152 3346
Size: 399 252 395 455 90 24 146 194
Cuts at: 3346 3747 4554 4575 4629 4668 4683 4883 5625
Size: 401 807 21 54 39 15 200 742
Cuts at: 5625 5710 6781 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 807 742 455 401 399 395
252 200 194 146 90 85 54 39
24 21 15
BspEI T'CCGG_A
Cuts at: 2608 2797 7227 2608
Size: 189 4430 2945
Fragments arranged by size:
4430 2945 189
BspHI T'CATG_A
Cuts at: 5453 7183 5453
Size: 1730 5834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 3762 4919 844
Size: 1535 1383 1157 3489
Fragments arranged by size:
3489 1535 1383 1157
BsrBI CCG'CTC
Cuts at: 1394 1823 2179 3712 3943 3973 4039 4054 4060
Size: 429 356 1533 231 30 66 15 6
Cuts at: 4060 4177 5451 1394
Size: 117 1274 3507
Fragments arranged by size:
3507 1533 1274 429 356 231 117 66
30 15 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 4257 6059 6233 65
Size: 1183 3009 1802 174 1396
Fragments arranged by size:
3009 1802 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2947 6208 6395 1700
Size: 110 1137 3261 187 2869
Fragments arranged by size:
3261 2869 1137 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7564
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7564
BssSI C'ACGA_G
Cuts at: 5624 6636 5624
Size: 1012 6552
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7564
BstBI TT'CG_AA
Cuts at: 6368 6368
Size: 7564
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7564
BstXI CCAn_nnnn'nTGG
Cuts at: 4391 4391
Size: 7564
BstYI r'GATC_y
Cuts at: 1972 2212 3268 3900 4894 5261 5646 5663 7115
Size: 240 1056 632 994 367 385 17 1452
Cuts at: 7115 7201 7213 7559 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1056 994 632 385 367 346
240 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2892 3018 4417 4423 4444 4599
Size: 552 939 888 126 1399 6 21 155
Cuts at: 4599 4693 513
Size: 94 3384
Fragments arranged by size:
3384 1399 939 888 552 155 126 94
21 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2922 487
Size: 683 56 1696 5129
Fragments arranged by size:
5129 1696 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 5080 5874 5894 2437
Size: 2643 794 20 4107
Fragments arranged by size:
4107 2643 794 20
ClaI AT'CG_AT
Cuts at: 1554 2112 1554
Size: 558 7006
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2724 2759 529
Size: 35 2160 35 5334
Fragments arranged by size:
5334 2160 35 35
DraI TTT'AAA
Cuts at: 4868 4868
Size: 7564
DraIII CAC_nnn'GTG
Cuts at: 4730 4753 4730
Size: 23 7541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6571 809
Size: 507 5255 1802
Fragments arranged by size:
5255 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2820 4229 4283
Size: 54 1425 50 275 18 990 1409 54
Cuts at: 4283 4420 4447 4534 4785 5011 5900 7529 8
Size: 137 27 87 251 226 889 1629 43
Fragments arranged by size:
1629 1425 1409 990 889 275 251 226
137 87 54 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 4447 5011 1537
Size: 275 2635 564 4090
Fragments arranged by size:
4090 2635 564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2196 2766 2796 3576 5494 1353
Size: 843 570 30 780 1918 3423
Fragments arranged by size:
3423 1918 843 780 570 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2138 4204 4779 4794 6151
Size: 1064 138 435 114 2066 575 15 1357
Cuts at: 6151 6525 6671 387
Size: 374 146 1280
Fragments arranged by size:
2066 1357 1280 1064 575 435 374 146
138 114 15
EcoICRI GAG'CTC
Cuts at: 2980 3004 3150 2980
Size: 24 146 7394
Fragments arranged by size:
7394 146 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2303 2384 2421 2436 2732 3636
Size: 393 518 153 81 37 15 296 904
Cuts at: 3636 4055 4085 5627 6224 7011 7279 1239
Size: 419 30 1542 597 787 268 1524
Fragments arranged by size:
1542 1524 904 787 597 518 419 393
296 268 153 81 37 30 15
EcoO109I rG'GnC_Cy
Cuts at: 2039 2776 4194 4430 4654 4665 2039
Size: 737 1418 236 224 11 4938
Fragments arranged by size:
4938 1418 737 236 224 11
EcoRI G'AATT_C
Cuts at: 4873 5251 4873
Size: 378 7186
EcoRV GAT'ATC
Cuts at: 4265 4265
Size: 7564
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2642 2642
Size: 7564
FspI TGC'GCA
Cuts at: 6070 6070
Size: 7564
HaeII r_GCGC'y
Cuts at: 1678 2793 3135 3880 4027 4147 4444 6711 1678
Size: 1115 342 745 147 120 297 2267 2531
Fragments arranged by size:
2531 2267 1115 745 342 297 147 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3420 3452 3732 3764 3787
Size: 32 135 32 1848 32 280 32 23
Cuts at: 3787 3794 3819 3824 3826 3856 6205 6237 6279
Size: 7 25 5 2 30 2349 32 42
Cuts at: 6279 6311 1373
Size: 32 2626
Fragments arranged by size:
2626 2349 1848 280 135 42 32 32
32 32 32 32 30 25 23 7
5 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3372 3529 4908 5154 669
Size: 988 281 894 540 157 1379 246 3079
Fragments arranged by size:
3079 1379 988 894 540 281 246 157
HindIII A'AGCT_T
Cuts at: 748 2759 3476 748
Size: 2011 717 4836
Fragments arranged by size:
4836 2011 717
HpaI GTT'AAC
Cuts at: 5154 5154
Size: 7564
KasI G'GCGC_C
Cuts at: 4143 4143
Size: 7564
KpnI G_GTAC'C
Cuts at: 4889 4889
Size: 7564
MfeI C'AATT_G
Cuts at: 5163 5163
Size: 7564
MluI A'CGCG_T
Cuts at: 7440 7440
Size: 7564
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3203 3507 4509 6678 6862 1305
Size: 1898 304 1002 2169 184 2007
Fragments arranged by size:
2169 2007 1898 1002 304 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 4231 4422 4787 10
Size: 54 1425 343 990 1409 191 365 2787
Fragments arranged by size:
2787 1425 1409 990 365 343 191 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 3755 4082 4389 5522 5881 6040
Size: 648 1233 1356 327 307 1133 359 159
Cuts at: 6040 518
Size: 2042
Fragments arranged by size:
2042 1356 1233 1133 648 359 327 307
159
MspA1I CmG'CkG
Cuts at: 1606 2512 2894 3213 3240 3889 3937 3997 4030
Size: 906 382 319 27 649 48 60 33
Cuts at: 4030 4159 4561 4695 4980 5657 6805 7050 1606
Size: 129 402 134 285 677 1148 245 2120
Fragments arranged by size:
2120 1148 906 677 649 402 382 319
285 245 134 129 60 48 33 27
NaeI GCC'GGC
Cuts at: 2949 2949
Size: 7564
NarI GG'CG_CC
Cuts at: 4144 4144
Size: 7564
NcoI C'CATG_G
Cuts at: 513 1065 2004 4417 4599 513
Size: 552 939 2413 182 3478
Fragments arranged by size:
3478 2413 939 552 182
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7564
NgoMIV G'CCGG_C
Cuts at: 2947 2947
Size: 7564
NheI G'CTAG_C
Cuts at: 1052 4616 1052
Size: 3564 4000
NotI GC'GGCC_GC
Cuts at: 5011 5011
Size: 7564
NspI r_CATG'y
Cuts at: 1132 1229 2546 4081 4922 6467 1132
Size: 97 1317 1535 841 1545 2229
Fragments arranged by size:
2229 1545 1535 1317 841 97
PasI CC'CwG_GG
Cuts at: 1348 4527 4741 1348
Size: 3179 214 4171
Fragments arranged by size:
4171 3179 214
PciI A'CATG_T
Cuts at: 6463 6463
Size: 7564
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2444 2909 4033 1108
Size: 1336 465 1124 4639
Fragments arranged by size:
4639 1336 1124 465
PmeI GTTT'AAAC
Cuts at: 4868 4868
Size: 7564
PmlI CAC'GTG
Cuts at: 4750 4750
Size: 7564
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5633 5665 7146 7178 5633
Size: 32 1481 32 6019
Fragments arranged by size:
6019 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2776 4430 2776
Size: 1654 5910
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7564
PsiI TTA'TAA
Cuts at: 5134 5134
Size: 7564
PspOMI G'GGCC_C
Cuts at: 2038 4664 2038
Size: 2626 4938
PspXI vC'TCGA_Gb
Cuts at: 3956 4013 3956
Size: 57 7507
PstI C_TGCA'G
Cuts at: 830 2376 4011 4916 830
Size: 1546 1635 905 3478
Fragments arranged by size:
3478 1635 1546 905
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7564
PvuII CAG'CTG
Cuts at: 2512 4159 2512
Size: 1647 5917
SacI G_AGCT'C
Cuts at: 2982 3006 3152 2982
Size: 24 146 7394
Fragments arranged by size:
7394 146 24
SacII CC_GC'GG
Cuts at: 2895 4696 2895
Size: 1801 5763
SalI G'TCGA_C
Cuts at: 1936 3370 3527 4906 1936
Size: 1434 157 1379 4594
Fragments arranged by size:
4594 1434 1379 157
SbfI CC_TGCA'GG
Cuts at: 4916 4916
Size: 7564
ScaI AGT'ACT
Cuts at: 5812 7524 5812
Size: 1712 5852
SfcI C'TryA_G
Cuts at: 826 1045 2372 2934 3992 4007 4912 6047 6728
Size: 219 1327 562 1058 15 905 1135 681
Cuts at: 6728 6919 826
Size: 191 1471
Fragments arranged by size:
1471 1327 1135 1058 905 681 562 219
191 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 4672 4672
Size: 7564
SfoI GGC'GCC
Cuts at: 4145 4145
Size: 7564
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7564
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7564
SmaI CCC'GGG
Cuts at: 1872 2023 2064 3339 4186 4891 1872
Size: 151 41 1275 847 705 4545
Fragments arranged by size:
4545 1275 847 705 151 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 3272 3956 4013 5668 6569
Size: 197 926 240 1089 684 57 1655 901
Cuts at: 6569 6831 820
Size: 262 1553
Fragments arranged by size:
1655 1553 1089 926 901 684 262 240
197 57
SnaBI TAC'GTA
Cuts at: 493 5355 493
Size: 4862 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7564
SphI G_CATG'C
Cuts at: 1132 2546 4081 4922 1132
Size: 1414 1535 841 3774
Fragments arranged by size:
3774 1535 1414 841
SrfI GCCC'GGGC
Cuts at: 2064 4186 2064
Size: 2122 5442
SspI AAT'ATT
Cuts at: 5 52 5488 5
Size: 47 5436 2081
Fragments arranged by size:
5436 2081 47
StuI AGG'CCT
Cuts at: 1517 2990 3962 1517
Size: 1473 972 5119
Fragments arranged by size:
5119 1473 972
StyI C'CwwG_G
Cuts at: 513 1065 2004 4417 4599 5003 513
Size: 552 939 2413 182 404 3074
Fragments arranged by size:
3074 2413 939 552 404 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3031 3663 4224 4275 4833 5591 5608
Size: 1255 1451 632 561 51 558 758 17
Cuts at: 5608 5761 5946 6391 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1451 1255 758 632 561 558 445
185 153 51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5810 7522 96
Size: 274 80 33 51 1443 3833 1712 138
Fragments arranged by size:
3833 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2911 2943 4230 4262 7524 7556 2911
Size: 32 1287 32 3262 32 2919
Fragments arranged by size:
3262 2919 1287 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7564
XbaI T'CTAG_A
Cuts at: 3677 4900 3677
Size: 1223 6341
XhoI C'TCGA_G
Cuts at: 1943 3956 4013 1943
Size: 2013 57 5494
Fragments arranged by size:
5494 2013 57
XmaI C'CCGG_G
Cuts at: 1870 2021 2062 3337 4184 4889 1870
Size: 151 41 1275 847 705 4545
Fragments arranged by size:
4545 1275 847 705 151 41
XmnI GAAnn'nnTTC
Cuts at: 3281 5693 3281
Size: 2412 5152
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5372 276
Size: 53 83 186 936 3838 2468
Fragments arranged by size:
3838 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI NruI NsiI
PacI PfoI PsrI RsrII SanDI SapI SexAI SwaI
XcmI